AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034468

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034468.t1 MTR_1g093095 75.000 116 26 1 132 244 99 214 3.66e-63 201
MS.gene034468.t1 MTR_4g059870 35.922 103 59 2 142 244 61 156 4.40e-12 66.2
MS.gene034468.t1 MTR_2g093960 32.727 110 67 2 135 244 48 150 1.71e-11 64.3
MS.gene034468.t1 MTR_1g016010 34.286 105 62 2 140 244 62 159 5.27e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034468.t1 AT1G08290 52.878 278 87 11 8 244 3 277 2.50e-80 246
MS.gene034468.t1 AT1G51220 50.000 248 86 6 19 244 43 274 1.61e-72 226
MS.gene034468.t1 AT3G20880 60.452 177 54 2 84 244 176 352 5.16e-71 224
MS.gene034468.t1 AT3G57670 66.000 150 40 2 95 244 175 313 7.27e-69 218
MS.gene034468.t1 AT1G34790 58.385 161 65 1 84 244 83 241 1.82e-63 201
MS.gene034468.t1 AT1G13290 59.509 163 47 4 84 244 54 199 6.91e-63 200
MS.gene034468.t1 AT1G34370 34.014 147 78 5 142 276 28 167 2.87e-15 74.7
MS.gene034468.t1 AT1G34370 36.364 110 60 3 142 243 238 345 4.11e-15 75.5
MS.gene034468.t1 AT1G34370 36.364 110 60 3 142 243 238 345 4.11e-15 75.5
MS.gene034468.t1 AT5G22890 35.185 108 64 2 142 243 211 318 1.40e-12 67.4
MS.gene034468.t1 AT5G03150 33.654 104 62 2 141 244 75 171 1.02e-11 65.1
MS.gene034468.t1 AT3G13810 32.727 110 67 2 135 244 87 189 6.46e-11 62.8
MS.gene034468.t1 AT3G13810 32.727 110 67 2 135 244 86 188 6.57e-11 62.8
MS.gene034468.t1 AT3G13810 32.727 110 67 2 135 244 86 188 6.57e-11 62.8
MS.gene034468.t1 AT3G13810 32.727 110 67 2 135 244 81 183 6.91e-11 62.8
MS.gene034468.t1 AT3G13810 32.727 110 67 2 135 244 73 175 7.18e-11 62.8
MS.gene034468.t1 AT4G02670 33.636 110 66 2 135 244 69 171 7.39e-11 62.4
MS.gene034468.t1 AT4G02670 33.636 110 66 2 135 244 71 173 7.42e-11 62.4
MS.gene034468.t1 AT3G13810 32.727 110 67 2 135 244 67 169 8.04e-11 62.4
MS.gene034468.t1 AT5G60470 35.294 102 59 2 142 243 58 152 8.18e-11 62.4
MS.gene034468.t1 AT5G60470 35.294 102 59 2 142 243 67 161 8.76e-11 62.4
MS.gene034468.t1 AT5G60470 35.294 102 59 2 142 243 63 157 9.64e-11 62.4

Find 48 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAAAGCATTTGCTGTTAA+AGG 0.122625 2.3:+12657598 MS.gene034468:CDS
TTGAGCCATTCAATGAAATT+TGG 0.134972 2.3:-12654733 None:intergenic
CATCCCATGGTTGTCCTCAT+AGG 0.237924 2.3:-12654863 None:intergenic
TTGCTAAGCCATGAAATTAA+TGG 0.248141 2.3:+12658242 MS.gene034468:CDS
ACAATTCTCCATTAATTTCA+TGG 0.264188 2.3:-12658250 None:intergenic
AGTTTGTGTTGATGAGTTAA+AGG 0.287140 2.3:-12654793 None:intergenic
TGAGCCATTCAATGAAATTT+GGG 0.289191 2.3:-12654732 None:intergenic
GATGATGAGAGTGAGGAATT+AGG 0.300962 2.3:-12654760 None:intergenic
AAAGGACCAGACTCACTTAA+AGG 0.304674 2.3:+12657412 MS.gene034468:CDS
AAACCCCAAATTTCATTGAA+TGG 0.361241 2.3:+12654728 MS.gene034468:CDS
ATTGAAAGTCTTACTGCATA+TGG 0.368036 2.3:-12655130 None:intergenic
TGAACATGATGAGAAGAATA+AGG 0.376871 2.3:+12654981 MS.gene034468:CDS
AATGGAGAATTGTTTGCTAC+TGG 0.377265 2.3:+12658260 MS.gene034468:CDS
ACTCCTGCTCAAATTCTTGT+TGG 0.396733 2.3:+12655087 MS.gene034468:CDS
TTAAGGAAGAGAGAAGGTTT+TGG 0.404432 2.3:+12655058 MS.gene034468:CDS
TGTGTTCCTTTAAGTGAGTC+TGG 0.407901 2.3:-12657418 None:intergenic
GATTCATGACCTCCTATGTT+AGG 0.421115 2.3:-12654955 None:intergenic
GGCTTAATTCCACTAGAGAT+TGG 0.432908 2.3:-12654841 None:intergenic
CAATGAAATTTGGGGTTTGA+AGG 0.444233 2.3:-12654723 None:intergenic
TCCAAGAGCAAAACCATTGA+AGG 0.445743 2.3:+12657507 MS.gene034468:CDS
ATCCCATGGTTGTCCTCATA+GGG 0.446171 2.3:-12654862 None:intergenic
CTAGAATTAAGATGGGTTTG+AGG 0.448542 2.3:+12654668 None:intergenic
TAGAATTAAGATGGGTTTGA+GGG 0.453608 2.3:+12654669 None:intergenic
AGAGTTCTGAAATCCTTCAA+TGG 0.482663 2.3:-12657520 None:intergenic
ATGGAGAATTGTTTGCTACT+GGG 0.497730 2.3:+12658261 MS.gene034468:CDS
CCATGTTATTGTTGTGCTCA+CGG 0.500524 2.3:+12657463 MS.gene034468:CDS
ATCAGTGATGATAAGCTAGC+AGG 0.503324 2.3:+12658293 MS.gene034468:CDS
TGAAGTAAAAGAAGAGAAGG+TGG 0.512589 2.3:+12654900 MS.gene034468:CDS
TGATGAGTTAAAGGCGATGA+AGG 0.525454 2.3:-12654784 None:intergenic
TGAAGGAGATGATGAGAGTG+AGG 0.539316 2.3:-12654767 None:intergenic
ACAAGAATTTGAGCAGGAGT+TGG 0.541700 2.3:-12655084 None:intergenic
CACATTGGATTGCCTAACAT+AGG 0.547171 2.3:+12654943 MS.gene034468:CDS
CACAACCAGCAGCAATGCTA+AGG 0.552239 2.3:+12657437 MS.gene034468:CDS
GATGAGAGTGAGGAATTAGG+TGG 0.553504 2.3:-12654757 None:intergenic
ACTCATTACAAAAGAAAGCA+TGG 0.557148 2.3:+12657547 MS.gene034468:CDS
TAGTGGAATTAAGCCCTATG+AGG 0.559838 2.3:+12654849 MS.gene034468:CDS
CAATAGATACAACAATATGC+AGG 0.564922 2.3:+12655149 MS.gene034468:CDS
GGTAGCCTTAGCATTGCTGC+TGG 0.575071 2.3:-12657442 None:intergenic
TCAGTGATGATAAGCTAGCA+GGG 0.587456 2.3:+12658294 MS.gene034468:CDS
GGTCCAACAAGAATTTGAGC+AGG 0.591899 2.3:-12655090 None:intergenic
GTGACTGTGACTTTGCACAT+TGG 0.592096 2.3:+12654928 MS.gene034468:CDS
AAGCCCTATGAGGACAACCA+TGG 0.608837 2.3:+12654859 MS.gene034468:CDS
ATGGAGCAAGCAAACTGCAT+AGG 0.613273 2.3:-12655111 None:intergenic
GAGCCATTCAATGAAATTTG+GGG 0.617863 2.3:-12654731 None:intergenic
ATTGGATTGCCTAACATAGG+AGG 0.621227 2.3:+12654946 MS.gene034468:CDS
AGCCCTATGAGGACAACCAT+GGG 0.622008 2.3:+12654860 MS.gene034468:CDS
CCGTGAGCACAACAATAACA+TGG 0.635068 2.3:-12657463 None:intergenic
CATGGATCAGAATTTAGGAA+AGG 0.639859 2.3:+12657394 MS.gene034468:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAATTTAATATGACTA+AGG + chr2.3:12656192-12656211 MS.gene034468:intron 10.0%
!! TTAGTCATATTAAATTATAT+TGG - chr2.3:12656193-12656212 None:intergenic 10.0%
!!! ATTTTATCTCTATATTATTT+GGG + chr2.3:12657213-12657232 MS.gene034468:intron 10.0%
!!! TATTTTATCTCTATATTATT+TGG + chr2.3:12657212-12657231 MS.gene034468:intron 10.0%
!! AATGTATAATACAACTATTA+GGG + chr2.3:12656481-12656500 MS.gene034468:intron 15.0%
!! ATAATAGTGACATTTATTTA+AGG + chr2.3:12656822-12656841 MS.gene034468:intron 15.0%
!! GATATAAAAATTAGTTACAT+AGG - chr2.3:12658040-12658059 None:intergenic 15.0%
!! TAATAGTGACATTTATTTAA+GGG + chr2.3:12656823-12656842 MS.gene034468:intron 15.0%
!! TATTATATATATAGCATGTT+AGG - chr2.3:12658112-12658131 None:intergenic 15.0%
!!! AAAATTTTATAGAGTCTAAA+AGG - chr2.3:12656917-12656936 None:intergenic 15.0%
!!! ATAAATTTGAAATAAAGTGA+CGG - chr2.3:12655902-12655921 None:intergenic 15.0%
!!! ATATTAATGGTTTTAAATTG+CGG - chr2.3:12658085-12658104 None:intergenic 15.0%
!! AAAACCTAAGTACAAATTAA+GGG - chr2.3:12656514-12656533 None:intergenic 20.0%
!! AATGTTTGATCTAATTCATA+AGG - chr2.3:12656656-12656675 None:intergenic 20.0%
!! AATTCATATTTAGTAGTCTT+AGG + chr2.3:12655367-12655386 MS.gene034468:intron 20.0%
!! ACTATTGAAGTGATAAAATA+TGG - chr2.3:12655688-12655707 None:intergenic 20.0%
!! CAATGTATAATACAACTATT+AGG + chr2.3:12656480-12656499 MS.gene034468:intron 20.0%
!! TAAAACCTAAGTACAAATTA+AGG - chr2.3:12656515-12656534 None:intergenic 20.0%
!! TAAGTATAATTATGTGACTT+TGG - chr2.3:12656088-12656107 None:intergenic 20.0%
!! TAAGTCAAGATTATAAAGAT+AGG + chr2.3:12655530-12655549 MS.gene034468:intron 20.0%
!! TATATTGTGATAAAATGCAA+CGG + chr2.3:12657870-12657889 MS.gene034468:intron 20.0%
!!! AAAAAAATTGTGGAAAGTTT+TGG + chr2.3:12657638-12657657 MS.gene034468:intron 20.0%
!!! AAGGTGTGATTTTATTTTAT+AGG - chr2.3:12656898-12656917 None:intergenic 20.0%
!!! AAGTGTGATTTTATTTTGTA+TGG + chr2.3:12657055-12657074 MS.gene034468:intron 20.0%
!!! AGGTGTGATTTTATTTTATA+GGG - chr2.3:12656897-12656916 None:intergenic 20.0%
!!! ATTTTTTCCTAAAAATCGAA+GGG - chr2.3:12655285-12655304 None:intergenic 20.0%
!!! CATGATATGATTTTAAATTG+AGG + chr2.3:12657824-12657843 MS.gene034468:intron 20.0%
!!! GTTTTATCAAGAATTATTCA+AGG + chr2.3:12655341-12655360 MS.gene034468:intron 20.0%
!!! TAAATTTTCATCAACACTTT+TGG + chr2.3:12656994-12657013 MS.gene034468:intron 20.0%
!!! TATTTTTTCCTAAAAATCGA+AGG - chr2.3:12655286-12655305 None:intergenic 20.0%
!!! TCACATTTTTTAAAGTGTAA+AGG - chr2.3:12656325-12656344 None:intergenic 20.0%
!!! TTTGGTTTTTCTTATGTAAT+TGG - chr2.3:12656766-12656785 None:intergenic 20.0%
! AAAAATGTGAGCAAAATGAA+TGG + chr2.3:12656335-12656354 MS.gene034468:intron 25.0%
! AAATAGCATAGTCTATAGTT+GGG - chr2.3:12655770-12655789 None:intergenic 25.0%
! ACAATTCTCCATTAATTTCA+TGG - chr2.3:12658253-12658272 None:intergenic 25.0%
! AGAACTCATGAAAAAAATTG+TGG + chr2.3:12657628-12657647 MS.gene034468:intron 25.0%
! AGGTGTTTAACATTATGATT+AGG + chr2.3:12656212-12656231 MS.gene034468:intron 25.0%
! CAAAAGAAAAGAAAACCTAA+GGG - chr2.3:12655403-12655422 None:intergenic 25.0%
! CAAAATTCTGAATATTTACG+AGG - chr2.3:12656866-12656885 None:intergenic 25.0%
! CAAGACTGTGAATATTTATA+GGG + chr2.3:12657086-12657105 MS.gene034468:intron 25.0%
! CAATAGTTCAATTAATTGCA+TGG + chr2.3:12655701-12655720 MS.gene034468:intron 25.0%
! CTATTATATCATCATCATCA+TGG - chr2.3:12656809-12656828 None:intergenic 25.0%
! GAAAGCAAAAATAAATGAGA+GGG - chr2.3:12656303-12656322 None:intergenic 25.0%
! GAAAGCAAATTTCTCTTATA+GGG + chr2.3:12655422-12655441 MS.gene034468:intron 25.0%
! GATAAAAAAGATATCGTTCA+AGG + chr2.3:12656551-12656570 MS.gene034468:intron 25.0%
! TAAAAACTGGAAAGTTTCTT+GGG - chr2.3:12654821-12654840 None:intergenic 25.0%
! TAAATAGCATAGTCTATAGT+TGG - chr2.3:12655771-12655790 None:intergenic 25.0%
! TAAGAAGTTATGTGTATGAA+TGG - chr2.3:12655664-12655683 None:intergenic 25.0%
! TCAAAAGAAAAGAAAACCTA+AGG - chr2.3:12655404-12655423 None:intergenic 25.0%
! TCAAGACTGTGAATATTTAT+AGG + chr2.3:12657085-12657104 MS.gene034468:intron 25.0%
! TCTAATAGTAATTACGATTG+CGG + chr2.3:12658058-12658077 MS.gene034468:intron 25.0%
! TGAAAGCAAATTTCTCTTAT+AGG + chr2.3:12655421-12655440 MS.gene034468:intron 25.0%
! TGTTTAACATTATGATTAGG+AGG + chr2.3:12656215-12656234 MS.gene034468:intron 25.0%
!! AAGATATGGTCTTTTACTAT+AGG - chr2.3:12656422-12656441 None:intergenic 25.0%
!! AATGATTGTTTTCTTCTTGA+TGG - chr2.3:12656251-12656270 None:intergenic 25.0%
!! ACATTTTCCTTAAGCATTTT+AGG - chr2.3:12657022-12657041 None:intergenic 25.0%
!! CATTTTCCTTAAGCATTTTA+GGG - chr2.3:12657021-12657040 None:intergenic 25.0%
!! GCTGATGGAAAAATTTTATA+GGG - chr2.3:12656957-12656976 None:intergenic 25.0%
!! TGCTGATGGAAAAATTTTAT+AGG - chr2.3:12656958-12656977 None:intergenic 25.0%
!!! TAAGGTTTTTGATTGTGTTA+AGG + chr2.3:12654999-12655018 MS.gene034468:CDS 25.0%
!!! TCTTAACCCTTCTATTTTTA+GGG + chr2.3:12655214-12655233 MS.gene034468:intron 25.0%
!!! TTCTTAACCCTTCTATTTTT+AGG + chr2.3:12655213-12655232 MS.gene034468:intron 25.0%
!!! TTTTGAAGTAAAAGAAGAGA+AGG + chr2.3:12654897-12654916 MS.gene034468:CDS 25.0%
!!! TTTTTTTGACAAAACAGCAT+AGG - chr2.3:12655964-12655983 None:intergenic 25.0%
AAACCCCAAATTTCATTGAA+TGG + chr2.3:12654728-12654747 MS.gene034468:CDS 30.0%
AAAGATCATGTTAGATCCTT+CGG + chr2.3:12657703-12657722 MS.gene034468:intron 30.0%
ACAATATGCAGGTTAGTAAT+TGG + chr2.3:12655160-12655179 MS.gene034468:intron 30.0%
ACTCATTACAAAAGAAAGCA+TGG + chr2.3:12657547-12657566 MS.gene034468:CDS 30.0%
ATAGGGACAAAAAATGATGA+TGG - chr2.3:12656940-12656959 None:intergenic 30.0%
ATAGTAACGAAGGATATAGA+GGG - chr2.3:12656047-12656066 None:intergenic 30.0%
ATATCGGAAAGGAAATACAA+AGG - chr2.3:12656449-12656468 None:intergenic 30.0%
ATTGAAAGTCTTACTGCATA+TGG - chr2.3:12655133-12655152 None:intergenic 30.0%
ATTTCCTTTCCGATATCAAA+GGG + chr2.3:12656453-12656472 MS.gene034468:intron 30.0%
ATTTGTCATCATAGTAACGA+AGG - chr2.3:12656057-12656076 None:intergenic 30.0%
CAATAGATACAACAATATGC+AGG + chr2.3:12655149-12655168 MS.gene034468:CDS 30.0%
CATGTTAGGCATGATATTAA+TGG - chr2.3:12658098-12658117 None:intergenic 30.0%
GGAAAGCAAAAATAAATGAG+AGG - chr2.3:12656304-12656323 None:intergenic 30.0%
GTAAAAACTGGAAAGTTTCT+TGG - chr2.3:12654822-12654841 None:intergenic 30.0%
TATCATCATCATCATGGATA+TGG - chr2.3:12656803-12656822 None:intergenic 30.0%
TATTTCCTTTCCGATATCAA+AGG + chr2.3:12656452-12656471 MS.gene034468:intron 30.0%
TGAACATGATGAGAAGAATA+AGG + chr2.3:12654981-12655000 MS.gene034468:CDS 30.0%
TGAGCCATTCAATGAAATTT+GGG - chr2.3:12654735-12654754 None:intergenic 30.0%
TTAGGTCAATAATTTCCCTT+AGG + chr2.3:12655385-12655404 MS.gene034468:intron 30.0%
TTCTAATCAGATAAGAGAGA+CGG + chr2.3:12655564-12655583 MS.gene034468:intron 30.0%
TTGAGCCATTCAATGAAATT+TGG - chr2.3:12654736-12654755 None:intergenic 30.0%
TTGCTAAGCCATGAAATTAA+TGG + chr2.3:12658242-12658261 MS.gene034468:CDS 30.0%
! AAGAGCCTTTTACTAAAAGT+TGG - chr2.3:12656389-12656408 None:intergenic 30.0%
! AATACAAAGGCATGAAGATA+TGG - chr2.3:12656436-12656455 None:intergenic 30.0%
! AGAGCCTTTTACTAAAAGTT+GGG - chr2.3:12656388-12656407 None:intergenic 30.0%
! AGTTTGTGTTGATGAGTTAA+AGG - chr2.3:12654796-12654815 None:intergenic 30.0%
! CTCTATATTATTTGGGACTT+AGG + chr2.3:12657220-12657239 MS.gene034468:intron 30.0%
! CTTTTCCCCTAAAAATAGAA+GGG - chr2.3:12655223-12655242 None:intergenic 30.0%
! GAACATTTTCTGCAGATAAA+TGG - chr2.3:12657580-12657599 None:intergenic 30.0%
! GTATCCCAACTTTTAGTAAA+AGG + chr2.3:12656381-12656400 MS.gene034468:intron 30.0%
! TCTTTTCCCCTAAAAATAGA+AGG - chr2.3:12655224-12655243 None:intergenic 30.0%
! TTCTTAACCCTTCGATTTTT+AGG + chr2.3:12655275-12655294 MS.gene034468:intron 30.0%
!! AAAACCACTTAGTTTTTGGA+AGG - chr2.3:12657167-12657186 None:intergenic 30.0%
!! AAACCACTTAGTTTTTGGAA+GGG - chr2.3:12657166-12657185 None:intergenic 30.0%
!! ATCACCCTTAATTTGTACTT+AGG + chr2.3:12656507-12656526 MS.gene034468:intron 30.0%
!! GAGTAAAACCACTTAGTTTT+TGG - chr2.3:12657171-12657190 None:intergenic 30.0%
!! TCTAAAGCATTTGCTGTTAA+AGG + chr2.3:12657598-12657617 MS.gene034468:CDS 30.0%
!!! CTTAACCCTTCTATTTTTAG+GGG + chr2.3:12655215-12655234 MS.gene034468:intron 30.0%
!!! TTTGTTTTAAGGAAGAGAGA+AGG + chr2.3:12655052-12655071 MS.gene034468:CDS 30.0%
ACAGAGACTATAAATGCTGA+TGG - chr2.3:12656972-12656991 None:intergenic 35.0%
ATGCTTAAGGAAAATGTGAC+AGG + chr2.3:12657025-12657044 MS.gene034468:intron 35.0%
CACTAGAGATTGGTAAAAAC+TGG - chr2.3:12654834-12654853 None:intergenic 35.0%
CATAGTAACGAAGGATATAG+AGG - chr2.3:12656048-12656067 None:intergenic 35.0%
CATGGATCAGAATTTAGGAA+AGG + chr2.3:12657394-12657413 MS.gene034468:CDS 35.0%
GAATTGAAGAAAAACGTGCA+TGG + chr2.3:12655027-12655046 MS.gene034468:CDS 35.0%
GAGCCATTCAATGAAATTTG+GGG - chr2.3:12654734-12654753 None:intergenic 35.0%
TCATGTTAAACTAGCATAGG+AGG - chr2.3:12655998-12656017 None:intergenic 35.0%
TGAAGTAAAAGAAGAGAAGG+TGG + chr2.3:12654900-12654919 MS.gene034468:CDS 35.0%
TGCTTAAGGAAAATGTGACA+GGG + chr2.3:12657026-12657045 MS.gene034468:intron 35.0%
TTGCGTATGAAGTATGAACT+AGG - chr2.3:12655630-12655649 None:intergenic 35.0%
TTTGGTCCCTAAAATGCTTA+AGG + chr2.3:12657012-12657031 MS.gene034468:intron 35.0%
! AATCTTCATTTTGCATCCCA+TGG - chr2.3:12654879-12654898 None:intergenic 35.0%
! AATGGAGAATTGTTTGCTAC+TGG + chr2.3:12658260-12658279 MS.gene034468:CDS 35.0%
! AGAGTTGATCATCATGAGAT+TGG + chr2.3:12656704-12656723 MS.gene034468:intron 35.0%
! ATGGAGAATTGTTTGCTACT+GGG + chr2.3:12658261-12658280 MS.gene034468:CDS 35.0%
! ATGGTTCTTGATTAGCTCTT+TGG - chr2.3:12656784-12656803 None:intergenic 35.0%
! ATTTTGCAGATGCATATGTG+GGG + chr2.3:12657370-12657389 MS.gene034468:intron 35.0%
! CAATGAAATTTGGGGTTTGA+AGG - chr2.3:12654726-12654745 None:intergenic 35.0%
! CAGTTTTTACCAATCTCTAG+TGG + chr2.3:12654832-12654851 MS.gene034468:CDS 35.0%
! CATTTTGCAGATGCATATGT+GGG + chr2.3:12657369-12657388 MS.gene034468:intron 35.0%
! GAGTTGATCATCATGAGATT+GGG + chr2.3:12656705-12656724 MS.gene034468:intron 35.0%
! TAAAATGCAACGGATTTGTG+CGG + chr2.3:12657880-12657899 MS.gene034468:intron 35.0%
! TTAAGGAAGAGAGAAGGTTT+TGG + chr2.3:12655058-12655077 MS.gene034468:CDS 35.0%
! TTTTGACAAAACAGCATAGG+AGG - chr2.3:12655961-12655980 None:intergenic 35.0%
!! AAGTTTTGGTATTGCACTTG+TGG + chr2.3:12657652-12657671 MS.gene034468:intron 35.0%
!! AGAGTTCTGAAATCCTTCAA+TGG - chr2.3:12657523-12657542 None:intergenic 35.0%
!! CATTTGCTGTTAAAGGTGAT+TGG + chr2.3:12657605-12657624 MS.gene034468:intron 35.0%
!!! CGTGCATGGTTTTTGTTTTA+AGG + chr2.3:12655041-12655060 MS.gene034468:CDS 35.0%
!!! GCTTTTTTCGGAATGTACAA+CGG + chr2.3:12657242-12657261 MS.gene034468:intron 35.0%
!!! TTTGGGACTTAGGCTTTTTT+CGG + chr2.3:12657230-12657249 MS.gene034468:intron 35.0%
AAAGGACCAGACTCACTTAA+AGG + chr2.3:12657412-12657431 MS.gene034468:CDS 40.0%
ACAAGAATTTGAGCAGGAGT+TGG - chr2.3:12655087-12655106 None:intergenic 40.0%
ACTCCTGCTCAAATTCTTGT+TGG + chr2.3:12655087-12655106 MS.gene034468:CDS 40.0%
ATCAGTGATGATAAGCTAGC+AGG + chr2.3:12658293-12658312 MS.gene034468:CDS 40.0%
ATTAGCTGTGATCGAATACG+TGG - chr2.3:12657268-12657287 None:intergenic 40.0%
ATTGGATTGCCTAACATAGG+AGG + chr2.3:12654946-12654965 MS.gene034468:CDS 40.0%
CACATTGGATTGCCTAACAT+AGG + chr2.3:12654943-12654962 MS.gene034468:CDS 40.0%
CATGTTAGATCCTTCGGAAA+AGG + chr2.3:12657709-12657728 MS.gene034468:intron 40.0%
CATTGCACACCCTTTGATAT+CGG - chr2.3:12656465-12656484 None:intergenic 40.0%
CCACATATGCATCTGCAAAA+TGG - chr2.3:12657371-12657390 None:intergenic 40.0%
CCATGTTATTGTTGTGCTCA+CGG + chr2.3:12657463-12657482 MS.gene034468:CDS 40.0%
CTTAATTGTGCGTCTTGTGT+CGG + chr2.3:12655924-12655943 MS.gene034468:intron 40.0%
GAGAAAGAATGCAGCAATAC+TGG + chr2.3:12657769-12657788 MS.gene034468:intron 40.0%
GATGATGAGAGTGAGGAATT+AGG - chr2.3:12654763-12654782 None:intergenic 40.0%
GATTCATGACCTCCTATGTT+AGG - chr2.3:12654958-12654977 None:intergenic 40.0%
GATTTGTGCGGTTGAAATTG+AGG + chr2.3:12657892-12657911 MS.gene034468:intron 40.0%
GGCTTAATTCCACTAGAGAT+TGG - chr2.3:12654844-12654863 None:intergenic 40.0%
GGGTCATGTTAAACTAGCAT+AGG - chr2.3:12656001-12656020 None:intergenic 40.0%
TAAAAGTTGGGATACCCTAG+CGG - chr2.3:12656376-12656395 None:intergenic 40.0%
TAGTGGAATTAAGCCCTATG+AGG + chr2.3:12654849-12654868 MS.gene034468:CDS 40.0%
TCACCCTTCCAAAAACTAAG+TGG + chr2.3:12657160-12657179 MS.gene034468:intron 40.0%
TCAGTGATGATAAGCTAGCA+GGG + chr2.3:12658294-12658313 MS.gene034468:CDS 40.0%
TCCAAGAGCAAAACCATTGA+AGG + chr2.3:12657507-12657526 MS.gene034468:CDS 40.0%
TCTTCAAAGCCTTCAAGAGA+AGG - chr2.3:12657748-12657767 None:intergenic 40.0%
TGATGAGTTAAAGGCGATGA+AGG - chr2.3:12654787-12654806 None:intergenic 40.0%
TGTGTTCCTTTAAGTGAGTC+TGG - chr2.3:12657421-12657440 None:intergenic 40.0%
! CCATTTTGCAGATGCATATG+TGG + chr2.3:12657368-12657387 MS.gene034468:intron 40.0%
! TTTTGCAGATGCATATGTGG+GGG + chr2.3:12657371-12657390 MS.gene034468:intron 40.0%
!! TCCTTCAATGGTTTTGCTCT+TGG - chr2.3:12657511-12657530 None:intergenic 40.0%
AAAAGTTGGGATACCCTAGC+GGG - chr2.3:12656375-12656394 None:intergenic 45.0%
AAGCCTTCAAGAGAAGGAAG+TGG - chr2.3:12657742-12657761 None:intergenic 45.0%
AGCCTTCAAGAGAAGGAAGT+GGG - chr2.3:12657741-12657760 None:intergenic 45.0%
ATCCCATGGTTGTCCTCATA+GGG - chr2.3:12654865-12654884 None:intergenic 45.0%
ATGGAGCAAGCAAACTGCAT+AGG - chr2.3:12655114-12655133 None:intergenic 45.0%
CACACCCTTTGATATCGGAA+AGG - chr2.3:12656460-12656479 None:intergenic 45.0%
CCGTGAGCACAACAATAACA+TGG - chr2.3:12657466-12657485 None:intergenic 45.0%
GATGAGAGTGAGGAATTAGG+TGG - chr2.3:12654760-12654779 None:intergenic 45.0%
GGTCCAACAAGAATTTGAGC+AGG - chr2.3:12655093-12655112 None:intergenic 45.0%
GTGACTGTGACTTTGCACAT+TGG + chr2.3:12654928-12654947 MS.gene034468:CDS 45.0%
TGAAGGAGATGATGAGAGTG+AGG - chr2.3:12654770-12654789 None:intergenic 45.0%
!! ATGTAAAAATTATTAAATAA+AGG - chr2.3:12657130-12657149 None:intergenic 5.0%
!! TGTAAAAATTATTAAATAAA+GGG - chr2.3:12657129-12657148 None:intergenic 5.0%
!!! AAATTATTTTTTTTTTTTGA+AGG + chr2.3:12655797-12655816 MS.gene034468:intron 5.0%
!!! AATAATTAAATTTTATACAT+GGG - chr2.3:12656021-12656040 None:intergenic 5.0%
!!! TAATAATTAAATTTTATACA+TGG - chr2.3:12656022-12656041 None:intergenic 5.0%
AAGCCCTATGAGGACAACCA+TGG + chr2.3:12654859-12654878 MS.gene034468:CDS 50.0%
AGCCCTATGAGGACAACCAT+GGG + chr2.3:12654860-12654879 MS.gene034468:CDS 50.0%
CACAACCAGCAGCAATGCTA+AGG + chr2.3:12657437-12657456 MS.gene034468:CDS 50.0%
CATCCCATGGTTGTCCTCAT+AGG - chr2.3:12654866-12654885 None:intergenic 50.0%
GCCTTCAAGAGAAGGAAGTG+GGG - chr2.3:12657740-12657759 None:intergenic 50.0%
TCCCCACTTCCTTCTCTTGA+AGG + chr2.3:12657736-12657755 MS.gene034468:intron 50.0%
! GGGGGCATGGATCAGAATTT+AGG + chr2.3:12657389-12657408 MS.gene034468:CDS 50.0%
! TGGGGAATGACCTTTTCCGA+AGG - chr2.3:12657722-12657741 None:intergenic 50.0%
CAGATGCATATGTGGGGGCA+TGG + chr2.3:12657376-12657395 MS.gene034468:intron 55.0%
! GGTAGCCTTAGCATTGCTGC+TGG - chr2.3:12657445-12657464 None:intergenic 55.0%
TTGTTGTCCCGCACCCGCTA+GGG + chr2.3:12656359-12656378 MS.gene034468:intron 60.0%
! TTGGGATACCCTAGCGGGTG+CGG - chr2.3:12656370-12656389 None:intergenic 60.0%
! CTTGTTGTCCCGCACCCGCT+AGG + chr2.3:12656358-12656377 MS.gene034468:intron 65.0%
! TGGGATACCCTAGCGGGTGC+GGG - chr2.3:12656369-12656388 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 12654679 12658322 12654679 ID=MS.gene034468
chr2.3 mRNA 12654679 12658322 12654679 ID=MS.gene034468.t1;Parent=MS.gene034468
chr2.3 exon 12654679 12655170 12654679 ID=MS.gene034468.t1.exon1;Parent=MS.gene034468.t1
chr2.3 CDS 12654679 12655170 12654679 ID=cds.MS.gene034468.t1;Parent=MS.gene034468.t1
chr2.3 exon 12657379 12657619 12657379 ID=MS.gene034468.t1.exon2;Parent=MS.gene034468.t1
chr2.3 CDS 12657379 12657619 12657379 ID=cds.MS.gene034468.t1;Parent=MS.gene034468.t1
chr2.3 exon 12658222 12658322 12658222 ID=MS.gene034468.t1.exon3;Parent=MS.gene034468.t1
chr2.3 CDS 12658222 12658322 12658222 ID=cds.MS.gene034468.t1;Parent=MS.gene034468.t1
Gene Sequence

>MS.gene034468

ATGGGTTTGAGGGATCAGTGCAATGTAGAAGACAAGAATCTCTACCTTCAAACCCCAAATTTCATTGAATGGCTCAAACCACCTAATTCCTCACTCTCATCATCTCCTTCATCGCCTTTAACTCATCAACACAAACTATCCCAAGAAACTTTCCAGTTTTTACCAATCTCTAGTGGAATTAAGCCCTATGAGGACAACCATGGGATGCAAAATGAAGATTTTGAAGTAAAAGAAGAGAAGGTGGAACATGTGACTGTGACTTTGCACATTGGATTGCCTAACATAGGAGGTCATGAATCTGATGAACATGATGAGAAGAATAAGGTTTTTGATTGTGTTAAGGAAGAAGAATTGAAGAAAAACGTGCATGGTTTTTGTTTTAAGGAAGAGAGAAGGTTTTGGATACCAACTCCTGCTCAAATTCTTGTTGGACCTATGCAGTTTGCTTGCTCCATATGCAGTAAGACTTTCAATAGATACAACAATATGCAGGTTAGTAATTGGTTCTCAATTTCTCATATTGATATATATTTTTTCTTAACCCTTCTATTTTTAGGGGAAAAGATTTTGATAATTTAAACTTCATATATTTTTTCTTCTTAACCCTTCGATTTTTAGGAAAAAATATTTTGATAATTTAAGTTCAATTGAGACGAAGTAAAGTTTTATCAAGAATTATTCAAGGCATAATTCATATTTAGTAGTCTTAGGTCAATAATTTCCCTTAGGTTTTCTTTTCTTTTGAAAGCAAATTTCTCTTATAGGGATAAAAGTTAATTAGAATTTCTGTTACAGTATATTATTTACTCATAATTTAACATATGTGCAAAGAACAAATATACACTTATTTTTAAGTCAAGATTATAAAGATAGGATCAATAATCATTCTAATCAGATAAGAGAGACGGAGATATTGAAAATTAAGCAATCATGCATGTTTTTTCATATCCTAGTTCATACTTCATACGCAACTTTTTTCTTTCCATTCATACACATAACTTCTTATCCATATTTTATCACTTCAATAGTTCAATTAATTGCATGGTAATTAAGTACTTAATTGATGAAATAAATCGATCATGTCTTCACCCAACTATAGACTATGCTATTTAAGTTCAAAATTATTTTTTTTTTTTGAAGGAAAGTTCAAAATTATTGATATTATTTTTTAAAAACAATCTATTGATTAAGTACATGAATAAGTTTTTCTTGAACATATACCGTCACTTTATTTCAAATTTATTTCTTAATTGTGCGTCTTGTGTCGGTTAATAGTTCTCCTCCTATGCTGTTTTGTCAAAAAAACAAAATGTTATCCTCCTATGCTAGTTTAACATGACCCATGTATAAAATTTAATTATTAAACCCTCTATATCCTTCGTTACTATGATGACAAATTTCTGCGTCCAAAGTCACATAATTATACTTACTAATAATGCACATCTCTATTTTTTTTTTTTTTTGTGACATGAATGTGCATTTAGAACTTGAATTATTTACTCTATATATATCCAATATAATTTAATATGACTAAGGTGTTTAACATTATGATTAGGAGGACGAAGATAACCATCAAGAAGAAAACAATCATTCAATTAAATGACTAATCTTTGATGCATGACCCTCTCATTTATTTTTGCTTTCCTTTACACTTTAAAAAATGTGAGCAAAATGAATGGCTTGTTGTCCCGCACCCGCTAGGGTATCCCAACTTTTAGTAAAAGGCTCTTTCAGTTTTATCCTATAGTAAAAGACCATATCTTCATGCCTTTGTATTTCCTTTCCGATATCAAAGGGTGTGCAATGTATAATACAACTATTAGGGTATATCACCCTTAATTTGTACTTAGGTTTTAATGTACTTCTTTTGTAGATAAAAAAGATATCGTTCAAGGTTTCTTGTATTAGTCATAGCTTTGAGAATAATTTGACTATTTTTTTGACAGAATAATTTGACTAGTTAATTAGTTAGATCCTTATGAATTAGATCAAACATTTTTTTATATGAAATAGTTATACTAAGACAGAGTTGATCATCATGAGATTGGGATATCATCATGAGACAGAGTTCAAGAATACTCAATCCAATTACATAAGAAAAACCAAAGAGCTAATCAAGAACCATATCCATGATGATGATGATATAATAGTGACATTTATTTAAGGGTTAATTAAGTAAATAGTCCTCGTAAATATTCAGAATTTTGTTTTTAGTCCCTATAAAATAAAATCACACCTTTTAGACTCTATAAAATTTTCCATCATCATTTTTTGTCCCTATAAAATTTTTCCATCAGCATTTATAGTCTCTGTCATAAATTTTCATCAACACTTTTGGTCCCTAAAATGCTTAAGGAAAATGTGACAGGGACTAAAAAGTGTGATTTTATTTTGTATGGACTAAAATCAAGACTGTGAATATTTATAGGGACTAAAAACTTAATTAACCCTTTATTTAATAATTTTTACATTTGATTTAACTCACCCTTCCAAAAACTAAGTGGTTTTACTCATTATAAACTTAATTATTTCATATTTTATCTCTATATTATTTGGGACTTAGGCTTTTTTCGGAATGTACAACGGCCACGTATTCGATCACAGCTAATTTTGAATTTGACTTAAAAACTATGAAATCAGAATTCAATGAACGTGTTAGCTCAAAAACTCAAACTAATCTTAAATTTGACCATTTTGCAGATGCATATGTGGGGGCATGGATCAGAATTTAGGAAAGGACCAGACTCACTTAAAGGAACACAACCAGCAGCAATGCTAAGGCTACCATGTTATTGTTGTGCTCACGGTTGCAAGAACAACATCAATCATCCAAGAGCAAAACCATTGAAGGATTTCAGAACTCTTCAAACTCATTACAAAAGAAAGCATGGAACAAAACCATTTATCTGCAGAAAATGTTCTAAAGCATTTGCTGTTAAAGGTGATTGGAGAACTCATGAAAAAAATTGTGGAAAGTTTTGGTATTGCACTTGTGGTTCTGATTTTAAACACAAAAGATCTCTTAAAGATCATGTTAGATCCTTCGGAAAAGGTCATTCCCCACTTCCTTCTCTTGAAGGCTTTGAAGATGAGAAAGAATGCAGCAATACTGGATCTGATGATGACGAAGTTGCTCATGCATGATCATGATATGATTTTAAATTGAGGTTGCAGTCATAGTTACAAACTTGTATATTGTGATAAAATGCAACGGATTTGTGCGGTTGAAATTGAGGTTGCGATGTTGTTATAAAGACATCGTAATCTTCTATATTGCACTCGTAACTATTAGATGTAAAAGTTAATTACACTGATTAGTTAGTTACACTACTTAGTTATAACTAAGTAGTGTAACTGACCTATGTAACTAATTTTTATATCTAATAGTAATTACGATTGCGGACCGCAATTTAAAACCATTAATATCATGCCTAACATGCTATATATATAATACATCTATCATATACTATATAAAGAGTAGTAGTTTATTTAGTAATAACTAGAAATTGTAGCAAAGCTTGATCATTGTGTGCTTGTGCTCAGTAACAAAAAATTCTCAACAATTGCTAAGCCATGAAATTAATGGAGAATTGTTTGCTACTGGGCATTTGCTGATCAGTGATGATAAGCTAGCAGGGAATTAA
Protein sequence

>MS.gene034468.t1

MGLRDQCNVEDKNLYLQTPNFIEWLKPPNSSLSSSPSSPLTHQHKLSQETFQFLPISSGIKPYEDNHGMQNEDFEVKEEKVEHVTVTLHIGLPNIGGHESDEHDEKNKVFDCVKEEELKKNVHGFCFKEERRFWIPTPAQILVGPMQFACSICSKTFNRYNNMQMHMWGHGSEFRKGPDSLKGTQPAAMLRLPCYCCAHGCKNNINHPRAKPLKDFRTLQTHYKRKHGTKPFICRKCSKAFAVKVTKNSQQLLSHEINGELFATGHLLISDDKLAGN