AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35396

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35396.t1 MTR_1g093095 77.707 157 32 1 132 285 99 255 7.04e-94 280
MS.gene35396.t1 MTR_4g059870 38.129 139 77 4 142 279 61 191 3.20e-20 91.3
MS.gene35396.t1 MTR_2g093960 35.616 146 85 4 135 279 48 185 1.04e-19 89.4
MS.gene35396.t1 MTR_1g016010 36.170 141 81 4 140 279 62 194 5.18e-19 87.4
MS.gene35396.t1 MTR_2g090745 34.932 146 85 5 135 279 23 159 3.04e-18 85.1
MS.gene35396.t1 MTR_1g112270 34.694 147 84 5 135 279 60 196 3.08e-18 85.5
MS.gene35396.t1 MTR_1g112270 34.694 147 84 5 135 279 71 207 3.62e-18 85.1
MS.gene35396.t1 MTR_1g094115 34.694 147 84 5 135 279 63 199 4.74e-18 84.7
MS.gene35396.t1 MTR_8g017210 35.714 140 78 6 142 279 85 214 5.82e-18 84.3
MS.gene35396.t1 MTR_1g094115 36.496 137 75 5 145 279 56 182 7.35e-18 84.3
MS.gene35396.t1 MTR_2g099990 34.694 147 84 6 135 279 62 198 1.54e-17 83.2
MS.gene35396.t1 MTR_2g099990 36.296 135 74 6 147 279 5 129 1.11e-16 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35396.t1 AT1G08290 57.910 335 95 12 10 301 5 336 9.59e-119 345
MS.gene35396.t1 AT1G51220 52.412 311 109 6 19 307 43 336 1.52e-103 306
MS.gene35396.t1 AT3G20880 63.158 228 68 2 84 295 176 403 5.64e-102 305
MS.gene35396.t1 AT3G57670 66.995 203 55 3 95 297 175 365 5.72e-100 299
MS.gene35396.t1 AT1G34790 59.729 221 85 2 84 304 83 299 1.02e-93 280
MS.gene35396.t1 AT1G13290 64.532 203 53 4 84 284 54 239 1.81e-92 277
MS.gene35396.t1 AT1G34370 39.241 158 84 5 142 290 28 182 2.29e-28 111
MS.gene35396.t1 AT1G34370 39.241 158 84 5 142 290 238 392 8.36e-28 112
MS.gene35396.t1 AT1G34370 39.241 158 84 5 142 290 238 392 8.36e-28 112
MS.gene35396.t1 AT5G22890 39.073 151 84 4 142 285 211 360 1.41e-24 102
MS.gene35396.t1 AT5G03150 36.691 139 79 4 142 279 76 206 2.93e-20 91.3
MS.gene35396.t1 AT1G55110 35.915 142 80 5 140 279 84 216 8.73e-19 86.7
MS.gene35396.t1 AT1G55110 35.915 142 80 5 140 279 84 216 8.73e-19 86.7
MS.gene35396.t1 AT1G55110 35.915 142 80 5 140 279 84 216 8.73e-19 86.7
MS.gene35396.t1 AT5G66730 35.915 142 79 5 140 279 53 184 1.28e-18 86.7
MS.gene35396.t1 AT5G60470 37.410 139 78 4 142 279 58 188 1.39e-18 86.3
MS.gene35396.t1 AT5G60470 37.410 139 78 4 142 279 63 193 1.60e-18 85.9
MS.gene35396.t1 AT5G60470 37.410 139 78 4 142 279 67 197 1.61e-18 85.9
MS.gene35396.t1 AT2G02080 35.461 141 81 4 140 279 75 206 1.61e-18 86.3
MS.gene35396.t1 AT2G02080 35.461 141 81 4 140 279 75 206 1.61e-18 86.3
MS.gene35396.t1 AT2G02080 35.461 141 81 4 140 279 75 206 1.61e-18 86.3
MS.gene35396.t1 AT2G02080 35.461 141 81 4 140 279 75 206 1.61e-18 86.3
MS.gene35396.t1 AT4G02670 35.616 146 85 4 135 279 71 208 1.94e-18 85.5
MS.gene35396.t1 AT4G02670 35.616 146 85 4 135 279 69 206 2.11e-18 85.5
MS.gene35396.t1 AT3G45260 36.691 139 79 4 142 279 62 192 2.60e-18 85.5
MS.gene35396.t1 AT3G45260 36.691 139 79 4 142 279 62 192 2.60e-18 85.5
MS.gene35396.t1 AT2G02070 34.932 146 85 4 135 279 68 204 3.56e-18 85.5
MS.gene35396.t1 AT2G02070 34.932 146 85 4 135 279 68 204 3.56e-18 85.5
MS.gene35396.t1 AT2G02080 36.567 134 75 4 147 279 5 129 5.43e-18 84.3
MS.gene35396.t1 AT2G02080 36.567 134 75 4 147 279 5 129 5.43e-18 84.3
MS.gene35396.t1 AT1G14580 34.247 146 86 4 135 279 69 205 5.51e-18 84.3
MS.gene35396.t1 AT1G14580 34.247 146 86 4 135 279 69 205 5.51e-18 84.3
MS.gene35396.t1 AT1G14580 34.247 146 86 4 135 279 83 219 6.12e-18 84.3
MS.gene35396.t1 AT1G03840 34.932 146 85 5 135 279 55 191 1.28e-17 83.6
MS.gene35396.t1 AT1G03840 34.932 146 85 5 135 279 57 193 1.41e-17 83.6
MS.gene35396.t1 AT5G44160 33.813 139 82 4 142 279 60 189 1.56e-17 83.2
MS.gene35396.t1 AT5G44160 32.877 146 88 4 135 279 14 150 1.73e-17 82.8
MS.gene35396.t1 AT3G13810 34.247 146 87 4 135 279 86 223 2.06e-17 82.8
MS.gene35396.t1 AT3G13810 34.247 146 87 4 135 279 86 223 2.06e-17 82.8
MS.gene35396.t1 AT3G13810 34.247 146 87 4 135 279 81 218 2.17e-17 82.8
MS.gene35396.t1 AT3G13810 34.247 146 87 4 135 279 67 204 2.26e-17 82.8
MS.gene35396.t1 AT3G13810 34.247 146 87 4 135 279 87 224 2.48e-17 82.8
MS.gene35396.t1 AT3G13810 34.247 146 87 4 135 279 73 210 2.70e-17 82.4
MS.gene35396.t1 AT3G50700 34.247 146 86 4 135 279 50 186 2.80e-17 82.4
MS.gene35396.t1 AT1G68130 31.655 139 84 5 140 275 62 192 1.40e-12 68.2
MS.gene35396.t1 AT1G25250 29.710 138 89 4 140 275 31 162 1.86e-12 67.8
MS.gene35396.t1 AT1G25250 29.710 138 89 4 140 275 54 185 2.49e-12 67.4
MS.gene35396.t1 AT2G01940 30.216 139 86 5 140 275 65 195 4.94e-11 63.5

Find 53 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAAAGCATTTGCTGTTAA+AGG 0.122625 2.1:+15829332 MS.gene35396:CDS
TTGAGCCATTCAATGAAATT+TGG 0.134972 2.1:-15826464 None:intergenic
CATCCCATGGTTGTCCTCAT+AGG 0.237924 2.1:-15826594 None:intergenic
TCCCCACTTCCTTCTCTTGA+AGG 0.285807 2.1:+15829470 MS.gene35396:CDS
AGTTTGTGTTGATGAGTTAA+AGG 0.287140 2.1:-15826524 None:intergenic
TGAGCCATTCAATGAAATTT+GGG 0.289191 2.1:-15826463 None:intergenic
GATGATGAGAGTGAGGAATT+AGG 0.300962 2.1:-15826491 None:intergenic
AAAGGACCAGACTCACTTAA+AGG 0.304674 2.1:+15829146 MS.gene35396:CDS
GAGAAAGAATGCAGCAATAC+TGG 0.341279 2.1:+15829503 MS.gene35396:CDS
AAACCCCAAATTTCATTGAA+TGG 0.361241 2.1:+15826459 MS.gene35396:CDS
ATTGAAAGTCTTACTGCATA+TGG 0.368036 2.1:-15826861 None:intergenic
TGAACATGATGAGAAGAATA+AGG 0.376871 2.1:+15826712 MS.gene35396:CDS
CATTTGCTGTTAAAGGTGAT+TGG 0.376909 2.1:+15829339 MS.gene35396:CDS
ACTCCTGCTCAAATTCTTGT+TGG 0.396733 2.1:+15826818 MS.gene35396:CDS
TTAAGGAAGAGAGAAGGTTT+TGG 0.404432 2.1:+15826789 MS.gene35396:CDS
TGTGTTCCTTTAAGTGAGTC+TGG 0.407901 2.1:-15829152 None:intergenic
AAAGATCATGTTAGATCCTT+CGG 0.408333 2.1:+15829437 MS.gene35396:CDS
GATTCATGACCTCCTATGTT+AGG 0.421115 2.1:-15826686 None:intergenic
TTGGTATTGCACTTGTGGTT+CGG 0.434745 2.1:+15829391 MS.gene35396:CDS
CAATGAAATTTGGGGTTTGA+AGG 0.444233 2.1:-15826454 None:intergenic
ATCCCATGGTTGTCCTCATA+GGG 0.446171 2.1:-15826593 None:intergenic
CACAACCAGCAGCAATGTTA+AGG 0.447930 2.1:+15829171 MS.gene35396:CDS
CTAGAATTAAGATGGGTTTG+AGG 0.448542 2.1:+15826399 None:intergenic
TAGAATTAAGATGGGTTTGA+GGG 0.453608 2.1:+15826400 None:intergenic
TCCAAGAGCAAAACCATTGA+AGG 0.462319 2.1:+15829241 MS.gene35396:CDS
AGAGTTCTGAAGTCCTTCAA+TGG 0.479319 2.1:-15829254 None:intergenic
CATGTTAGATCCTTCGGAAA+AGG 0.486223 2.1:+15829443 MS.gene35396:CDS
CCATGTTATTGTTGTGCTCA+CGG 0.500524 2.1:+15829197 MS.gene35396:CDS
GGGCTTAATTCCACTAGAGA+TGG 0.505824 2.1:-15826573 None:intergenic
TGAAGTAAAAGAAGAGAAGG+TGG 0.512589 2.1:+15826631 MS.gene35396:CDS
TCTTCAAAGCCTTCAAGAGA+AGG 0.517348 2.1:-15829479 None:intergenic
TGATGAGTTAAAGGCGATGA+AGG 0.525454 2.1:-15826515 None:intergenic
TGAAGGAGATGATGAGAGTG+AGG 0.539316 2.1:-15826498 None:intergenic
ACAAGAATTTGAGCAGGAGT+TGG 0.541700 2.1:-15826815 None:intergenic
CACATTGGATTGCCTAACAT+AGG 0.547171 2.1:+15826674 MS.gene35396:CDS
GATGAGAGTGAGGAATTAGG+TGG 0.553504 2.1:-15826488 None:intergenic
ACTCATTACAAAAGAAAGCA+TGG 0.557148 2.1:+15829281 MS.gene35396:CDS
TAGTGGAATTAAGCCCTATG+AGG 0.559838 2.1:+15826580 MS.gene35396:CDS
CAATAGATACAACAATATGC+AGG 0.564922 2.1:+15826880 MS.gene35396:CDS
AGCCTTCAAGAGAAGGAAGT+GGG 0.568332 2.1:-15829472 None:intergenic
GGTAGCCTTAACATTGCTGC+TGG 0.572110 2.1:-15829176 None:intergenic
GGCTTAATTCCACTAGAGAT+GGG 0.587730 2.1:-15826572 None:intergenic
GGTCCAACAAGAATTTGAGC+AGG 0.591899 2.1:-15826821 None:intergenic
GTGACTGTGACTTTGCACAT+TGG 0.592096 2.1:+15826659 MS.gene35396:CDS
AAGCCTTCAAGAGAAGGAAG+TGG 0.595192 2.1:-15829473 None:intergenic
AAGCCCTATGAGGACAACCA+TGG 0.608837 2.1:+15826590 MS.gene35396:CDS
ATGGAGCAAGCAAACTGCAT+AGG 0.613273 2.1:-15826842 None:intergenic
GAGCCATTCAATGAAATTTG+GGG 0.617863 2.1:-15826462 None:intergenic
ATTGGATTGCCTAACATAGG+AGG 0.621227 2.1:+15826677 MS.gene35396:CDS
AGCCCTATGAGGACAACCAT+GGG 0.622008 2.1:+15826591 MS.gene35396:CDS
CCGTGAGCACAACAATAACA+TGG 0.635068 2.1:-15829197 None:intergenic
CATGGATCAGAATTTAGGAA+AGG 0.639859 2.1:+15829128 MS.gene35396:CDS
GCCTTCAAGAGAAGGAAGTG+GGG 0.663895 2.1:-15829471 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAATTTAATATGACTA+AGG + chr2.1:15827923-15827942 MS.gene35396:intron 10.0%
!! TTAGTCATATTAAATTATAT+TGG - chr2.1:15827924-15827943 None:intergenic 10.0%
!!! ATTTTATCTCTATATTATTT+GGG + chr2.1:15828947-15828966 MS.gene35396:intron 10.0%
!!! TATTTTATCTCTATATTATT+TGG + chr2.1:15828946-15828965 MS.gene35396:intron 10.0%
!! AATGTATAATACAACTATTA+GGG + chr2.1:15828212-15828231 MS.gene35396:intron 15.0%
!! ATAATAGTGACATTTATTTA+AGG + chr2.1:15828556-15828575 MS.gene35396:intron 15.0%
!! TAATAGTGACATTTATTTAA+GGG + chr2.1:15828557-15828576 MS.gene35396:intron 15.0%
!!! ATAAATTTGAAATAAAGTGA+CGG - chr2.1:15827632-15827651 None:intergenic 15.0%
!! AAAACCTAAGTACAAATTAA+GGG - chr2.1:15828245-15828264 None:intergenic 20.0%
!! AATGTTTGATCTAATTCATA+AGG - chr2.1:15828387-15828406 None:intergenic 20.0%
!! AATTCATATTTAGTAGTCTT+AGG + chr2.1:15827098-15827117 MS.gene35396:intron 20.0%
!! ACAAAATTCTGAATATTTAC+AGG - chr2.1:15828601-15828620 None:intergenic 20.0%
!! ACTATTGAAGTGATAAAATA+TGG - chr2.1:15827419-15827438 None:intergenic 20.0%
!! CAAAATTCTGAATATTTACA+GGG - chr2.1:15828600-15828619 None:intergenic 20.0%
!! CAATGTATAATACAACTATT+AGG + chr2.1:15828211-15828230 MS.gene35396:intron 20.0%
!! TAAAACCTAAGTACAAATTA+AGG - chr2.1:15828246-15828265 None:intergenic 20.0%
!! TAAGTATAATTATGTGACTT+TGG - chr2.1:15827818-15827837 None:intergenic 20.0%
!! TAAGTCAAGATTATAAAGAT+AGG + chr2.1:15827261-15827280 MS.gene35396:intron 20.0%
!! TTAAGGGTTAATTAAGTAAA+TGG + chr2.1:15828573-15828592 MS.gene35396:intron 20.0%
!!! AAAAATTTCATCAACACTTT+TGG + chr2.1:15828728-15828747 MS.gene35396:intron 20.0%
!!! AAAATTTTATAGGGTCTAAA+AGG - chr2.1:15828651-15828670 None:intergenic 20.0%
!!! AAGGTGTGATTTTATTTTAT+AGG - chr2.1:15828632-15828651 None:intergenic 20.0%
!!! AAGTGTGATTTTATTTTGTA+TGG + chr2.1:15828789-15828808 MS.gene35396:intron 20.0%
!!! AGGTGTGATTTTATTTTATA+GGG - chr2.1:15828631-15828650 None:intergenic 20.0%
!!! ATTTTTTCCTAAAAATCGAA+GGG - chr2.1:15827016-15827035 None:intergenic 20.0%
!!! GTTTTATCAAGAATTATTCA+AGG + chr2.1:15827072-15827091 MS.gene35396:intron 20.0%
!!! TATTTTTTCCTAAAAATCGA+AGG - chr2.1:15827017-15827036 None:intergenic 20.0%
!!! TCACATTTTTTAAAGTGTAA+AGG - chr2.1:15828056-15828075 None:intergenic 20.0%
!!! TCTTAACCCTTATATTTTTA+GGG + chr2.1:15826945-15826964 MS.gene35396:intron 20.0%
!!! TTCTTAACCCTTATATTTTT+AGG + chr2.1:15826944-15826963 MS.gene35396:intron 20.0%
!!! TTTGGTTTTTCTTATGTAAT+TGG - chr2.1:15828497-15828516 None:intergenic 20.0%
! AAAAATGTGAGCAAAATGAA+TGG + chr2.1:15828066-15828085 MS.gene35396:intron 25.0%
! AAATAGCATAGTCTATAGTT+GGG - chr2.1:15827501-15827520 None:intergenic 25.0%
! AGGTGTTTAACATTATGATT+AGG + chr2.1:15827943-15827962 MS.gene35396:intron 25.0%
! CAAAAGAAAAGAAAACCTAA+GGG - chr2.1:15827134-15827153 None:intergenic 25.0%
! CAAGACTGTGAATATTTATA+GGG + chr2.1:15828820-15828839 MS.gene35396:intron 25.0%
! GAAAGCAAAAATAAATGAGA+GGG - chr2.1:15828034-15828053 None:intergenic 25.0%
! GAAAGCAAATTTCTCTTATA+GGG + chr2.1:15827153-15827172 MS.gene35396:intron 25.0%
! GATAAAAAAGATATCGTTCA+AGG + chr2.1:15828282-15828301 MS.gene35396:intron 25.0%
! TAAAAACTGGAAAGTTTCTT+GGG - chr2.1:15826552-15826571 None:intergenic 25.0%
! TAAATAGCATAGTCTATAGT+TGG - chr2.1:15827502-15827521 None:intergenic 25.0%
! TAAGAAGTTATGTGTATGAA+TGG - chr2.1:15827395-15827414 None:intergenic 25.0%
! TCAAAAGAAAAGAAAACCTA+AGG - chr2.1:15827135-15827154 None:intergenic 25.0%
! TCAAGACTGTGAATATTTAT+AGG + chr2.1:15828819-15828838 MS.gene35396:intron 25.0%
! TGAAAGCAAATTTCTCTTAT+AGG + chr2.1:15827152-15827171 MS.gene35396:intron 25.0%
! TGTTTAACATTATGATTAGG+AGG + chr2.1:15827946-15827965 MS.gene35396:intron 25.0%
! TTATATCATCATCATCATCA+TGG - chr2.1:15828540-15828559 None:intergenic 25.0%
!! AAAAAAACTGTGGAAAGTTT+TGG + chr2.1:15829372-15829391 MS.gene35396:CDS 25.0%
!! AAGATATGGTCTTTTACTAT+AGG - chr2.1:15828153-15828172 None:intergenic 25.0%
!! AATGATTGTTTTCTTCTTGA+TGG - chr2.1:15827982-15828001 None:intergenic 25.0%
!! ACATTTTCCTTAAGCATTTT+AGG - chr2.1:15828756-15828775 None:intergenic 25.0%
!! ATGCTGATGGAAAATTTTAT+AGG - chr2.1:15828661-15828680 None:intergenic 25.0%
!! CATTTTCCTTAAGCATTTTA+GGG - chr2.1:15828755-15828774 None:intergenic 25.0%
!! CTTTTCCCCTAAAAATATAA+GGG - chr2.1:15826954-15826973 None:intergenic 25.0%
!! GCTGATGGAAAAATTTTATA+GGG - chr2.1:15828691-15828710 None:intergenic 25.0%
!! TCTTTTCCCCTAAAAATATA+AGG - chr2.1:15826955-15826974 None:intergenic 25.0%
!! TGCTGATGGAAAAATTTTAT+AGG - chr2.1:15828692-15828711 None:intergenic 25.0%
!! TGCTGATGGAAAATTTTATA+GGG - chr2.1:15828660-15828679 None:intergenic 25.0%
!!! CTTAACCCTTATATTTTTAG+GGG + chr2.1:15826946-15826965 MS.gene35396:intron 25.0%
!!! TAAGGTTTTTGATTGTGTTA+AGG + chr2.1:15826730-15826749 MS.gene35396:CDS 25.0%
!!! TTTTGAAGTAAAAGAAGAGA+AGG + chr2.1:15826628-15826647 MS.gene35396:CDS 25.0%
!!! TTTTTTTGACAAAACAGCAT+AGG - chr2.1:15827694-15827713 None:intergenic 25.0%
AAACCCCAAATTTCATTGAA+TGG + chr2.1:15826459-15826478 MS.gene35396:CDS 30.0%
AAAGATCATGTTAGATCCTT+CGG + chr2.1:15829437-15829456 MS.gene35396:CDS 30.0%
ACAAAGACTATAAATGCTGA+TGG - chr2.1:15828706-15828725 None:intergenic 30.0%
ACAATATGCAGGTTAGTAAT+TGG + chr2.1:15826891-15826910 MS.gene35396:intron 30.0%
ACTCATTACAAAAGAAAGCA+TGG + chr2.1:15829281-15829300 MS.gene35396:CDS 30.0%
AGAACTCATGAAAAAAACTG+TGG + chr2.1:15829362-15829381 MS.gene35396:CDS 30.0%
ATAGTAACGAAGGATATAGA+GGG - chr2.1:15827777-15827796 None:intergenic 30.0%
ATATCGGAAAGGAAATACAA+AGG - chr2.1:15828180-15828199 None:intergenic 30.0%
ATTGAAAGTCTTACTGCATA+TGG - chr2.1:15826864-15826883 None:intergenic 30.0%
ATTTCCTTTCCGATATCAAA+GGG + chr2.1:15828184-15828203 MS.gene35396:intron 30.0%
ATTTGTCATCATAGTAACGA+AGG - chr2.1:15827787-15827806 None:intergenic 30.0%
CAATAGATACAACAATATGC+AGG + chr2.1:15826880-15826899 MS.gene35396:CDS 30.0%
GGAAAGCAAAAATAAATGAG+AGG - chr2.1:15828035-15828054 None:intergenic 30.0%
GTAAAAACTGGAAAGTTTCT+TGG - chr2.1:15826553-15826572 None:intergenic 30.0%
TATTTCCTTTCCGATATCAA+AGG + chr2.1:15828183-15828202 MS.gene35396:intron 30.0%
TGAACATGATGAGAAGAATA+AGG + chr2.1:15826712-15826731 MS.gene35396:CDS 30.0%
TGAGCCATTCAATGAAATTT+GGG - chr2.1:15826466-15826485 None:intergenic 30.0%
TTAGGTCAATAATTTCCCTT+AGG + chr2.1:15827116-15827135 MS.gene35396:intron 30.0%
TTCTAATCAGATAAGAGAGA+CGG + chr2.1:15827295-15827314 MS.gene35396:intron 30.0%
TTGAGCCATTCAATGAAATT+TGG - chr2.1:15826467-15826486 None:intergenic 30.0%
! AAGAGCCTTTTACTAAAAGT+TGG - chr2.1:15828120-15828139 None:intergenic 30.0%
! AATACAAAGGCATGAAGATA+TGG - chr2.1:15828167-15828186 None:intergenic 30.0%
! AGAGCCTTTTACTAAAAGTT+GGG - chr2.1:15828119-15828138 None:intergenic 30.0%
! AGTTTGTGTTGATGAGTTAA+AGG - chr2.1:15826527-15826546 None:intergenic 30.0%
! CTCTATATTATTTGGGACTT+AGG + chr2.1:15828954-15828973 MS.gene35396:intron 30.0%
! GAACATTTTCTGCAGATAAA+TGG - chr2.1:15829314-15829333 None:intergenic 30.0%
! GTATCCCAACTTTTAGTAAA+AGG + chr2.1:15828112-15828131 MS.gene35396:intron 30.0%
! TTCTTAACCCTTCGATTTTT+AGG + chr2.1:15827006-15827025 MS.gene35396:intron 30.0%
!! ATCACCCTTAATTTGTACTT+AGG + chr2.1:15828238-15828257 MS.gene35396:intron 30.0%
!! GAGTAAAACCACTTAGTTTT+TGG - chr2.1:15828905-15828924 None:intergenic 30.0%
!! TCTAAAGCATTTGCTGTTAA+AGG + chr2.1:15829332-15829351 MS.gene35396:CDS 30.0%
!!! GTTTTTTTCGGAATGTACAA+CGG + chr2.1:15828976-15828995 MS.gene35396:intron 30.0%
!!! TTTGGGACTTAGGTTTTTTT+CGG + chr2.1:15828964-15828983 MS.gene35396:intron 30.0%
!!! TTTGTTTTAAGGAAGAGAGA+AGG + chr2.1:15826783-15826802 MS.gene35396:CDS 30.0%
ATAGGGACTAAAAATGCTGA+TGG - chr2.1:15828674-15828693 None:intergenic 35.0%
ATGCTTAAGGAAAATGTGAC+AGG + chr2.1:15828759-15828778 MS.gene35396:intron 35.0%
ATTAGTTGTGATCGAATACG+TGG - chr2.1:15829002-15829021 None:intergenic 35.0%
CATAGTAACGAAGGATATAG+AGG - chr2.1:15827778-15827797 None:intergenic 35.0%
CATCATCATCATCATGGATA+TGG - chr2.1:15828534-15828553 None:intergenic 35.0%
CATGGATCAGAATTTAGGAA+AGG + chr2.1:15829128-15829147 MS.gene35396:CDS 35.0%
GAATTGAAGAAAAACGTGCA+TGG + chr2.1:15826758-15826777 MS.gene35396:CDS 35.0%
GAGCCATTCAATGAAATTTG+GGG - chr2.1:15826465-15826484 None:intergenic 35.0%
TCATGTTAAACTAGCATAGG+AGG - chr2.1:15827728-15827747 None:intergenic 35.0%
TGAAGTAAAAGAAGAGAAGG+TGG + chr2.1:15826631-15826650 MS.gene35396:CDS 35.0%
TGCTTAAGGAAAATGTGACA+GGG + chr2.1:15828760-15828779 MS.gene35396:intron 35.0%
TTGCGTATGAAGTATGAACT+AGG - chr2.1:15827361-15827380 None:intergenic 35.0%
TTTGGTCCCTAAAATGCTTA+AGG + chr2.1:15828746-15828765 MS.gene35396:intron 35.0%
! AATCTTCATTTTGCATCCCA+TGG - chr2.1:15826610-15826629 None:intergenic 35.0%
! AGAGTTGATCATCATGAGAT+TGG + chr2.1:15828435-15828454 MS.gene35396:intron 35.0%
! ATGGTTCTTGATTAGCTCTT+TGG - chr2.1:15828515-15828534 None:intergenic 35.0%
! ATTTTGCAGATGCATATGTG+GGG + chr2.1:15829104-15829123 MS.gene35396:intron 35.0%
! CAATGAAATTTGGGGTTTGA+AGG - chr2.1:15826457-15826476 None:intergenic 35.0%
! CATTTTGCAGATGCATATGT+GGG + chr2.1:15829103-15829122 MS.gene35396:intron 35.0%
! GAGTTGATCATCATGAGATT+GGG + chr2.1:15828436-15828455 MS.gene35396:intron 35.0%
! TCATTTTGCAGATGCATATG+TGG + chr2.1:15829102-15829121 MS.gene35396:intron 35.0%
! TTAAGGAAGAGAGAAGGTTT+TGG + chr2.1:15826789-15826808 MS.gene35396:CDS 35.0%
! TTTTGACAAAACAGCATAGG+AGG - chr2.1:15827691-15827710 None:intergenic 35.0%
!! AAGTTTTGGTATTGCACTTG+TGG + chr2.1:15829386-15829405 MS.gene35396:CDS 35.0%
!! CATTTGCTGTTAAAGGTGAT+TGG + chr2.1:15829339-15829358 MS.gene35396:CDS 35.0%
!!! CGTGCATGGTTTTTGTTTTA+AGG + chr2.1:15826772-15826791 MS.gene35396:CDS 35.0%
AAAGGACCAGACTCACTTAA+AGG + chr2.1:15829146-15829165 MS.gene35396:CDS 40.0%
ACAAGAATTTGAGCAGGAGT+TGG - chr2.1:15826818-15826837 None:intergenic 40.0%
ACTCCTGCTCAAATTCTTGT+TGG + chr2.1:15826818-15826837 MS.gene35396:CDS 40.0%
ATTGGATTGCCTAACATAGG+AGG + chr2.1:15826677-15826696 MS.gene35396:CDS 40.0%
CACATTGGATTGCCTAACAT+AGG + chr2.1:15826674-15826693 MS.gene35396:CDS 40.0%
CACTAGAGATGGGTAAAAAC+TGG - chr2.1:15826565-15826584 None:intergenic 40.0%
CATGTTAGATCCTTCGGAAA+AGG + chr2.1:15829443-15829462 MS.gene35396:CDS 40.0%
CATTGCACACCCTTTGATAT+CGG - chr2.1:15828196-15828215 None:intergenic 40.0%
CCATGTTATTGTTGTGCTCA+CGG + chr2.1:15829197-15829216 MS.gene35396:CDS 40.0%
CTTAATTGTGCGTCTTGTGT+CGG + chr2.1:15827654-15827673 MS.gene35396:intron 40.0%
GAGAAAGAATGCAGCAATAC+TGG + chr2.1:15829503-15829522 MS.gene35396:CDS 40.0%
GATGATGAGAGTGAGGAATT+AGG - chr2.1:15826494-15826513 None:intergenic 40.0%
GATTCATGACCTCCTATGTT+AGG - chr2.1:15826689-15826708 None:intergenic 40.0%
GGCTTAATTCCACTAGAGAT+GGG - chr2.1:15826575-15826594 None:intergenic 40.0%
GGGTCATGTTAAACTAGCAT+AGG - chr2.1:15827731-15827750 None:intergenic 40.0%
TAAAAGTTGGGATACCCTAG+CGG - chr2.1:15828107-15828126 None:intergenic 40.0%
TAGTGGAATTAAGCCCTATG+AGG + chr2.1:15826580-15826599 MS.gene35396:CDS 40.0%
TCACGCTTCCAAAAACTAAG+TGG + chr2.1:15828894-15828913 MS.gene35396:intron 40.0%
TCCAAGAGCAAAACCATTGA+AGG + chr2.1:15829241-15829260 MS.gene35396:CDS 40.0%
TCTTCAAAGCCTTCAAGAGA+AGG - chr2.1:15829482-15829501 None:intergenic 40.0%
TGATGAGTTAAAGGCGATGA+AGG - chr2.1:15826518-15826537 None:intergenic 40.0%
TGTGTTCCTTTAAGTGAGTC+TGG - chr2.1:15829155-15829174 None:intergenic 40.0%
TTGGTATTGCACTTGTGGTT+CGG + chr2.1:15829391-15829410 MS.gene35396:CDS 40.0%
! CAGTTTTTACCCATCTCTAG+TGG + chr2.1:15826563-15826582 MS.gene35396:CDS 40.0%
! TTTTGCAGATGCATATGTGG+GGG + chr2.1:15829105-15829124 MS.gene35396:intron 40.0%
!! AGAGTTCTGAAGTCCTTCAA+TGG - chr2.1:15829257-15829276 None:intergenic 40.0%
!! TCCTTCAATGGTTTTGCTCT+TGG - chr2.1:15829245-15829264 None:intergenic 40.0%
AAAAGTTGGGATACCCTAGC+GGG - chr2.1:15828106-15828125 None:intergenic 45.0%
AAGCCTTCAAGAGAAGGAAG+TGG - chr2.1:15829476-15829495 None:intergenic 45.0%
AGCCTTCAAGAGAAGGAAGT+GGG - chr2.1:15829475-15829494 None:intergenic 45.0%
ATCCCATGGTTGTCCTCATA+GGG - chr2.1:15826596-15826615 None:intergenic 45.0%
ATGGAGCAAGCAAACTGCAT+AGG - chr2.1:15826845-15826864 None:intergenic 45.0%
CACAACCAGCAGCAATGTTA+AGG + chr2.1:15829171-15829190 MS.gene35396:CDS 45.0%
CACACCCTTTGATATCGGAA+AGG - chr2.1:15828191-15828210 None:intergenic 45.0%
CCGTGAGCACAACAATAACA+TGG - chr2.1:15829200-15829219 None:intergenic 45.0%
GATGAGAGTGAGGAATTAGG+TGG - chr2.1:15826491-15826510 None:intergenic 45.0%
GGGCTTAATTCCACTAGAGA+TGG - chr2.1:15826576-15826595 None:intergenic 45.0%
GGTCCAACAAGAATTTGAGC+AGG - chr2.1:15826824-15826843 None:intergenic 45.0%
GTGACTGTGACTTTGCACAT+TGG + chr2.1:15826659-15826678 MS.gene35396:CDS 45.0%
TGAAGGAGATGATGAGAGTG+AGG - chr2.1:15826501-15826520 None:intergenic 45.0%
!! ATGTAAAAATTATTAAATAA+AGG - chr2.1:15828864-15828883 None:intergenic 5.0%
!! TGTAAAAATTATTAAATAAA+GGG - chr2.1:15828863-15828882 None:intergenic 5.0%
!!! AAATTATTTTATTTTTTTGA+AGG + chr2.1:15827528-15827547 MS.gene35396:intron 5.0%
!!! AATAATTAAATTTTATACAT+GGG - chr2.1:15827751-15827770 None:intergenic 5.0%
!!! TAATAATTAAATTTTATACA+TGG - chr2.1:15827752-15827771 None:intergenic 5.0%
AAGCCCTATGAGGACAACCA+TGG + chr2.1:15826590-15826609 MS.gene35396:CDS 50.0%
AGCCCTATGAGGACAACCAT+GGG + chr2.1:15826591-15826610 MS.gene35396:CDS 50.0%
CATCCCATGGTTGTCCTCAT+AGG - chr2.1:15826597-15826616 None:intergenic 50.0%
GCCTTCAAGAGAAGGAAGTG+GGG - chr2.1:15829474-15829493 None:intergenic 50.0%
TCCCCACTTCCTTCTCTTGA+AGG + chr2.1:15829470-15829489 MS.gene35396:CDS 50.0%
! GGGGGCATGGATCAGAATTT+AGG + chr2.1:15829123-15829142 MS.gene35396:CDS 50.0%
! GGTAGCCTTAACATTGCTGC+TGG - chr2.1:15829179-15829198 None:intergenic 50.0%
! TGGGGAATGACCTTTTCCGA+AGG - chr2.1:15829456-15829475 None:intergenic 50.0%
CAGATGCATATGTGGGGGCA+TGG + chr2.1:15829110-15829129 MS.gene35396:intron 55.0%
TTGGGATACCCTAGCGGGAG+CGG - chr2.1:15828101-15828120 None:intergenic 60.0%
TTGTTGTCCCGCTCCCGCTA+GGG + chr2.1:15828090-15828109 MS.gene35396:intron 60.0%
TGGGATACCCTAGCGGGAGC+GGG - chr2.1:15828100-15828119 None:intergenic 65.0%
! CTTGTTGTCCCGCTCCCGCT+AGG + chr2.1:15828089-15828108 MS.gene35396:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 15826410 15829556 15826410 ID=MS.gene35396
chr2.1 mRNA 15826410 15829556 15826410 ID=MS.gene35396.t1;Parent=MS.gene35396
chr2.1 exon 15826410 15826901 15826410 ID=MS.gene35396.t1.exon1;Parent=MS.gene35396.t1
chr2.1 CDS 15826410 15826901 15826410 ID=cds.MS.gene35396.t1;Parent=MS.gene35396.t1
chr2.1 exon 15829113 15829556 15829113 ID=MS.gene35396.t1.exon2;Parent=MS.gene35396.t1
chr2.1 CDS 15829113 15829556 15829113 ID=cds.MS.gene35396.t1;Parent=MS.gene35396.t1
Gene Sequence

>MS.gene35396

ATGGGTTTGAGGGATCAGTGCAATGTAGAAGACAAGAATCTCTACCTTCAAACCCCAAATTTCATTGAATGGCTCAAACCACCTAATTCCTCACTCTCATCATCTCCTTCATCGCCTTTAACTCATCAACACAAACTATCCCAAGAAACTTTCCAGTTTTTACCCATCTCTAGTGGAATTAAGCCCTATGAGGACAACCATGGGATGCAAAATGAAGATTTTGAAGTAAAAGAAGAGAAGGTGGAACATGTGACTGTGACTTTGCACATTGGATTGCCTAACATAGGAGGTCATGAATCTGATGAACATGATGAGAAGAATAAGGTTTTTGATTGTGTTAAGGAAGAAGAATTGAAGAAAAACGTGCATGGTTTTTGTTTTAAGGAAGAGAGAAGGTTTTGGATACCAACTCCTGCTCAAATTCTTGTTGGACCTATGCAGTTTGCTTGCTCCATATGCAGTAAGACTTTCAATAGATACAACAATATGCAGGTTAGTAATTGGTTCTCAATTTCTCATATTGATATATATTTTTTCTTAACCCTTATATTTTTAGGGGAAAAGATTTTGATAATTTAAACTTCATATATTTTTTCTTCTTAACCCTTCGATTTTTAGGAAAAAATATTTTGATAATTTAAGTTCAATTGAGACGAAGTAAAGTTTTATCAAGAATTATTCAAGGCATAATTCATATTTAGTAGTCTTAGGTCAATAATTTCCCTTAGGTTTTCTTTTCTTTTGAAAGCAAATTTCTCTTATAGGGATAAAAGTTAATTAGAATTTCTGTTACAGTATATTATTTACTCATAATTTAACTTGTGTGCAAAGAACAAATATACACTTATTTTTAAGTCAAGATTATAAAGATAGGATCAATAATCATTCTAATCAGATAAGAGAGACGGAGATATTGAAAATTAAGCAATCATGCATGTTTTTTCATATCCTAGTTCATACTTCATACGCAACTTTTTTCTTTCCATTCATACACATAACTTCTTATCCATATTTTATCACTTCAATAGTTCAATTAATTGCATGATAATTAAGTACTTAATTGATGAAATAAATCGATCATGTCTTCACCCAACTATAGACTATGCTATTTAAGTTCAAAATTATTTTATTTTTTTGAAGGAAAGTTCAAAATTATTGATATTATTTTTTAAAAAAATCTATTGATTGAGTACATGAATAAGTTTTTCTTGAACATATACCGTCACTTTATTTCAAATTTATTTCTTAATTGTGCGTCTTGTGTCGGTTAATAGTTCTCCTCCTATGCTGTTTTGTCAAAAAAACAAAATGTTATCCTCCTATGCTAGTTTAACATGACCCATGTATAAAATTTAATTATTAAACCCTCTATATCCTTCGTTACTATGATGACAAATTTCTGCGTCCAAAGTCACATAATTATACTTACTAATAATGCACATCTCTATTTTTTTTTTTTTTTTGTGACATGAATGTGCATTTAGAACTTGAATTATTTACTCTATATATATCCAATATAATTTAATATGACTAAGGTGTTTAACATTATGATTAGGAGGACGAAGATAACCATCAAGAAGAAAACAATCATTCAATTAAATGACTAATATTTGATGCATGACCCTCTCATTTATTTTTGCTTTCCTTTACACTTTAAAAAATGTGAGCAAAATGAATGGCTTGTTGTCCCGCTCCCGCTAGGGTATCCCAACTTTTAGTAAAAGGCTCTTTTAGTTTTATCCTATAGTAAAAGACCATATCTTCATGCCTTTGTATTTCCTTTCCGATATCAAAGGGTGTGCAATGTATAATACAACTATTAGGGTATATCACCCTTAATTTGTACTTAGGTTTTAATGTACTTCTTTTGTAGATAAAAAAGATATCGTTCAAGGTTTCTTGTATTAGTCATAGCTTTGAGAATAATTTGACTATTTTTTTGACAGAATAATTTGACTAGTTAATTAGTTAGATCCTTATGAATTAGATCAAACATTTTTTTATATGAAATAGTTATACTAAGACAGAGTTGATCATCATGAGATTGGGATATCATCATGAGACAGAGTTCAAGAATACTCAATCCAATTACATAAGAAAAACCAAAGAGCTAATCAAGAACCATATCCATGATGATGATGATGATATAATAGTGACATTTATTTAAGGGTTAATTAAGTAAATGGTCCCTGTAAATATTCAGAATTTTGTTTTTAGTCCCTATAAAATAAAATCACACCTTTTAGACCCTATAAAATTTTCCATCAGCATTTTTAGTCCCTATAAAATTTTTCCATCAGCATTTATAGTCTTTGTCAAAAAATTTCATCAACACTTTTGGTCCCTAAAATGCTTAAGGAAAATGTGACAGGGACTAAAAAGTGTGATTTTATTTTGTATGGACTAAAATCAAGACTGTGAATATTTATAGGGACTAAAAACTTAATTAACCCTTTATTTAATAATTTTTACATTTGATTTAACTCACGCTTCCAAAAACTAAGTGGTTTTACTCATTATAAACTTAATTATTTCATATTTTATCTCTATATTATTTGGGACTTAGGTTTTTTTCGGAATGTACAACGGCCACGTATTCGATCACAACTAATTTTGAATTTGACTTAAAAACTATGAAATCAGAATTCAATGAACGTGTTAGCTCAAAAACTCAAACTAATCTTAAATTTGATCATTTTGCAGATGCATATGTGGGGGCATGGATCAGAATTTAGGAAAGGACCAGACTCACTTAAAGGAACACAACCAGCAGCAATGTTAAGGCTACCATGTTATTGTTGTGCTCACGGTTGCAAGAACAACATCAATCATCCAAGAGCAAAACCATTGAAGGACTTCAGAACTCTTCAAACTCATTACAAAAGAAAGCATGGAACAAAACCATTTATCTGCAGAAAATGTTCTAAAGCATTTGCTGTTAAAGGTGATTGGAGAACTCATGAAAAAAACTGTGGAAAGTTTTGGTATTGCACTTGTGGTTCGGATTTTAAACACAAAAGATCTCTTAAAGATCATGTTAGATCCTTCGGAAAAGGTCATTCCCCACTTCCTTCTCTTGAAGGCTTTGAAGATGAGAAAGAATGCAGCAATACTGGATCTGATGATGACGAAGTAGCTCATGCATGA
Protein sequence

>MS.gene35396.t1

MGLRDQCNVEDKNLYLQTPNFIEWLKPPNSSLSSSPSSPLTHQHKLSQETFQFLPISSGIKPYEDNHGMQNEDFEVKEEKVEHVTVTLHIGLPNIGGHESDEHDEKNKVFDCVKEEELKKNVHGFCFKEERRFWIPTPAQILVGPMQFACSICSKTFNRYNNMQMHMWGHGSEFRKGPDSLKGTQPAAMLRLPCYCCAHGCKNNINHPRAKPLKDFRTLQTHYKRKHGTKPFICRKCSKAFAVKGDWRTHEKNCGKFWYCTCGSDFKHKRSLKDHVRSFGKGHSPLPSLEGFEDEKECSNTGSDDDEVAHA