AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020496


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020496.t1 MTR_8g104890 96.471 170 5 1 1 170 1 169 1.78e-121 339
MS.gene020496.t1 MTR_5g017650 60.795 176 49 4 1 156 1 176 4.17e-68 205
MS.gene020496.t1 MTR_8g104870 63.804 163 41 3 12 156 11 173 4.30e-66 200
MS.gene020496.t1 MTR_4g086330 51.205 166 70 2 14 170 34 197 7.70e-54 169
MS.gene020496.t1 MTR_8g104830 50.714 140 66 3 30 167 4 142 7.35e-43 140
MS.gene020496.t1 MTR_4g086320 56.667 120 51 1 25 144 42 160 1.18e-42 140
MS.gene020496.t1 MTR_2g101660 33.728 169 109 1 5 170 3 171 3.94e-31 110
MS.gene020496.t1 MTR_6g084940 43.066 137 74 3 34 170 17 149 1.37e-29 106
MS.gene020496.t1 MTR_1g075550 53.509 114 52 1 34 147 11 123 4.08e-26 97.1
MS.gene020496.t1 MTR_5g008240 39.416 137 68 4 21 142 27 163 4.81e-24 93.2
MS.gene020496.t1 MTR_5g008240 37.956 137 70 3 21 142 69 205 1.02e-23 93.2
MS.gene020496.t1 MTR_2g101660 36.585 123 75 1 51 170 1 123 1.11e-23 90.5
MS.gene020496.t1 MTR_5g008240 37.956 137 70 3 21 142 93 229 1.32e-23 93.6
MS.gene020496.t1 MTR_7g044840 31.655 139 65 3 28 137 53 190 4.94e-19 81.3
MS.gene020496.t1 MTR_0172s0050 32.414 145 64 4 29 140 57 200 8.80e-16 72.8
MS.gene020496.t1 MTR_3g408350 28.105 153 70 3 19 137 44 190 6.01e-15 70.1
MS.gene020496.t1 MTR_5g008240 40.741 108 49 4 50 142 38 145 1.30e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020496.t1 AT1G14870 63.946 147 50 3 26 170 7 152 3.74e-62 189
MS.gene020496.t1 AT5G35525 64.029 139 48 2 29 166 10 147 1.14e-59 182
MS.gene020496.t1 AT1G14870 67.227 119 39 0 26 144 7 125 1.97e-56 174
MS.gene020496.t1 AT1G14880 59.184 147 57 3 26 170 6 151 3.15e-54 169
MS.gene020496.t1 AT1G68610 56.934 137 56 2 33 166 17 153 6.31e-45 145
MS.gene020496.t1 AT1G49030 47.799 159 72 4 5 160 66 216 1.09e-44 147
MS.gene020496.t1 AT3G18450 47.682 151 72 4 10 160 33 176 4.34e-43 142
MS.gene020496.t1 AT3G18470 50.388 129 59 1 32 160 2 125 1.02e-42 139
MS.gene020496.t1 AT3G18460 50.345 145 65 3 18 162 42 179 1.34e-42 140
MS.gene020496.t1 AT1G58320 50.413 121 56 3 34 153 16 133 9.95e-37 124
MS.gene020496.t1 AT1G52200 40.116 172 93 4 9 170 19 190 1.36e-34 120
MS.gene020496.t1 AT1G52200 40.278 144 78 2 9 144 19 162 4.70e-29 106
MS.gene020496.t1 AT1G68630 38.849 139 80 2 28 162 18 155 2.70e-28 103
MS.gene020496.t1 AT2G40935 34.028 144 73 4 22 144 34 176 1.16e-21 87.0
MS.gene020496.t1 AT2G40935 34.028 144 73 4 22 144 34 176 1.16e-21 87.0
MS.gene020496.t1 AT2G40935 34.028 144 73 4 22 144 34 176 1.16e-21 87.0
MS.gene020496.t1 AT2G40935 34.028 144 73 4 22 144 34 176 1.16e-21 87.0
MS.gene020496.t1 AT2G40935 34.028 144 73 4 22 144 34 176 1.16e-21 87.0
MS.gene020496.t1 AT2G45010 33.103 145 66 4 28 142 9 152 7.62e-20 82.4
MS.gene020496.t1 AT2G45010 33.103 145 66 4 28 142 55 198 8.90e-20 83.2
MS.gene020496.t1 AT5G51400 30.921 152 73 5 19 140 47 196 2.36e-18 79.3
MS.gene020496.t1 AT2G40935 31.250 144 66 5 22 144 34 165 1.21e-15 71.2
MS.gene020496.t1 AT2G40935 35.345 116 53 4 50 144 38 152 1.28e-14 68.2
MS.gene020496.t1 AT2G40935 35.345 116 53 4 50 144 38 152 1.28e-14 68.2
MS.gene020496.t1 AT2G40935 35.345 116 53 4 50 144 38 152 1.28e-14 68.2

Find 47 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCATTCCTGGTTGGCATT+AGG 0.310976 4.1:+1932976 None:intergenic
GCCACCAGCCACCGGTTTCC+CGG 0.332845 4.1:-1933019 MS.gene020496:CDS
CCTGGTTGGCATTAGGTTGT+TGG 0.355979 4.1:+1932983 None:intergenic
ATAGACCCGTGGACCATTCC+TGG 0.365914 4.1:+1932965 None:intergenic
CGTGGATTTGACATGGAACT+TGG 0.398558 4.1:-1932458 MS.gene020496:intron
GGGTCTATTTGACTGCTTCT+CGG 0.401120 4.1:-1932950 MS.gene020496:CDS
ATTTGTTGTGTGACGGGTTG+TGG 0.418139 4.1:-1932617 MS.gene020496:CDS
TGGTGTCCATGCATCACCTT+TGG 0.426284 4.1:-1932799 MS.gene020496:CDS
TGTTGGTAGCCGACGTTCAC+CGG 0.431018 4.1:+1933000 None:intergenic
GCCGACGTTCACCGGGAAAC+CGG 0.448178 4.1:+1933008 None:intergenic
TTGCAGCTTGTGCTGTAAGC+GGG 0.457897 4.1:-1932655 MS.gene020496:intron
TACATTAATTTGTTGTGTGA+CGG 0.462544 4.1:-1932624 MS.gene020496:CDS
TGCTGGAGCTGTTGGTGCCA+TGG 0.464678 4.1:+1932041 None:intergenic
TGTTCAACTGCTGGAGCTGT+TGG 0.477230 4.1:+1932033 None:intergenic
CGAGTCATGTGTTCAACTGC+TGG 0.481564 4.1:+1932024 None:intergenic
ACATTAATTTGTTGTGTGAC+GGG 0.489462 4.1:-1932623 MS.gene020496:CDS
AAATGTGGCACAAGGGAACC+AGG 0.489547 4.1:-1932070 MS.gene020496:CDS
GTGGCTGGTGGCTGATTGTA+CGG 0.517478 4.1:+1933030 None:intergenic
TTGGTGCCATGGCTACTCCC+TGG 0.530182 4.1:+1932052 None:intergenic
AACCTAATGCCAACCAGGAA+TGG 0.530457 4.1:-1932978 MS.gene020496:CDS
TTCACCGGGAAACCGGTGGC+TGG 0.538277 4.1:+1933015 None:intergenic
GTTGGTAGCCGACGTTCACC+GGG 0.542435 4.1:+1933001 None:intergenic
GCCAACCAGGAATGGTCCAC+GGG 0.546652 4.1:-1932970 MS.gene020496:CDS
ACCGGGAAACCGGTGGCTGG+TGG 0.560600 4.1:+1933018 None:intergenic
TACAATCAGCCACCAGCCAC+CGG 0.567328 4.1:-1933027 MS.gene020496:CDS
ATTGCAGCTTGTGCTGTAAG+CGG 0.567979 4.1:-1932656 MS.gene020496:intron
AAATTGGCTATAGGGTGGCA+TGG 0.569669 4.1:-1932093 MS.gene020496:intron
CCAACAACCTAATGCCAACC+AGG 0.571791 4.1:-1932983 MS.gene020496:CDS
TGGCATGGAAATGTGGCACA+AGG 0.574561 4.1:-1932078 MS.gene020496:CDS
TGCCAACCAGGAATGGTCCA+CGG 0.582618 4.1:-1932971 MS.gene020496:CDS
TGCAGCTTGTGCTGTAAGCG+GGG 0.590429 4.1:-1932654 MS.gene020496:intron
AATCTCAGCAATTCGTCCAA+AGG 0.597233 4.1:+1932783 None:intergenic
ATTCGTCCAAAGGTGATGCA+TGG 0.598061 4.1:+1932793 None:intergenic
TGAAAACCGTGGATTTGACA+TGG 0.599587 4.1:-1932465 MS.gene020496:CDS
ACCCGTGGACCATTCCTGGT+TGG 0.600890 4.1:+1932969 None:intergenic
AATTGCTGAGATTGTTGACA+AGG 0.603304 4.1:-1932773 MS.gene020496:CDS
TATAGGGTGGCATGGAAATG+TGG 0.605383 4.1:-1932085 MS.gene020496:intron
ACCGGTTTCCCGGTGAACGT+CGG 0.612556 4.1:-1933009 MS.gene020496:CDS
AGGGAACCAGGGAGTAGCCA+TGG 0.614002 4.1:-1932058 MS.gene020496:CDS
CAAGTTCCATGTCAAATCCA+CGG 0.615456 4.1:+1932459 None:intergenic
AATGTGGCACAAGGGAACCA+GGG 0.650557 4.1:-1932069 MS.gene020496:CDS
GGCATGGAAATGTGGCACAA+GGG 0.652763 4.1:-1932077 MS.gene020496:CDS
GACGTTCACCGGGAAACCGG+TGG 0.653777 4.1:+1933011 None:intergenic
TGAACTAAGCAATCACAACA+AGG 0.663046 4.1:+1932533 None:intergenic
ATTGCTGAGATTGTTGACAA+GGG 0.678367 4.1:-1932772 MS.gene020496:CDS
TATCGTGAGCTTGAAAACCG+TGG 0.692905 4.1:-1932476 MS.gene020496:CDS
GAAGCAGTCAAATAGACCCG+TGG 0.701710 4.1:+1932954 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATTAAAATTTAGAAG+AGG + chr4.1:1932218-1932237 None:intergenic 10.0%
!!! TAAATTTCATTTCTTTTTTT+AGG - chr4.1:1932245-1932264 MS.gene020496:intron 10.0%
!!! TCTCTTAAATTTTTTATTTA+AGG - chr4.1:1932764-1932783 MS.gene020496:CDS 10.0%
!!! ATCTTAATTCTTTTATCTTT+TGG - chr4.1:1932679-1932698 MS.gene020496:intron 15.0%
!! AAGAGGAATTAATTAATGAA+CGG + chr4.1:1932201-1932220 None:intergenic 20.0%
!! CTTGTAAGATATTCATTAAT+TGG - chr4.1:1932336-1932355 MS.gene020496:intron 20.0%
!!! ATTTATTTGAAATTGGCTAT+AGG - chr4.1:1932978-1932997 MS.gene020496:CDS 20.0%
!!! TTTATTTGAAATTGGCTATA+GGG - chr4.1:1932979-1932998 MS.gene020496:CDS 20.0%
! GTTCTAATTACCACTTAATT+TGG - chr4.1:1932174-1932193 MS.gene020496:intron 25.0%
!! ATTGTTGTCTCATTTTGTTA+CGG + chr4.1:1932510-1932529 None:intergenic 25.0%
!!! TACATTAATTTGTTGTGTGA+CGG - chr4.1:1932456-1932475 MS.gene020496:intron 25.0%
! GGTTGTTGAAATTTTGAACT+TGG + chr4.1:1932032-1932051 None:intergenic 30.0%
! TCGACAATTCAATGTTTCTA+TGG - chr4.1:1932722-1932741 MS.gene020496:intron 30.0%
! TGTTGAAATTTTGAACTTGG+AGG + chr4.1:1932029-1932048 None:intergenic 30.0%
!! ACATTAATTTGTTGTGTGAC+GGG - chr4.1:1932457-1932476 MS.gene020496:intron 30.0%
AATGAACGGTCCAAATTAAG+TGG + chr4.1:1932187-1932206 None:intergenic 35.0%
AATTGCTGAGATTGTTGACA+AGG - chr4.1:1932307-1932326 MS.gene020496:intron 35.0%
ATTGCTGAGATTGTTGACAA+GGG - chr4.1:1932308-1932327 MS.gene020496:intron 35.0%
TGAACTAAGCAATCACAACA+AGG + chr4.1:1932550-1932569 None:intergenic 35.0%
! ATTTGAAATTGGCTATAGGG+TGG - chr4.1:1932982-1933001 MS.gene020496:CDS 35.0%
!!! TTTTAGGTTGCATAACGTTC+TGG - chr4.1:1932261-1932280 MS.gene020496:intron 35.0%
AATCTCAGCAATTCGTCCAA+AGG + chr4.1:1932300-1932319 None:intergenic 40.0%
ATTTGTACCATTGTCGCATC+TGG - chr4.1:1932912-1932931 MS.gene020496:intron 40.0%
CAAGTTCCATGTCAAATCCA+CGG + chr4.1:1932624-1932643 None:intergenic 40.0%
TGAAAACCGTGGATTTGACA+TGG - chr4.1:1932615-1932634 MS.gene020496:CDS 40.0%
AAATTGGCTATAGGGTGGCA+TGG - chr4.1:1932987-1933006 MS.gene020496:CDS 45.0%
AACCTAATGCCAACCAGGAA+TGG - chr4.1:1932102-1932121 MS.gene020496:intron 45.0%
AACTCATCCAGATGCGACAA+TGG + chr4.1:1932922-1932941 None:intergenic 45.0%
ATTCGTCCAAAGGTGATGCA+TGG + chr4.1:1932290-1932309 None:intergenic 45.0%
ATTGCAGCTTGTGCTGTAAG+CGG - chr4.1:1932424-1932443 MS.gene020496:intron 45.0%
CGTGGATTTGACATGGAACT+TGG - chr4.1:1932622-1932641 MS.gene020496:CDS 45.0%
GGGTCTATTTGACTGCTTCT+CGG - chr4.1:1932130-1932149 MS.gene020496:intron 45.0%
TATAGGGTGGCATGGAAATG+TGG - chr4.1:1932995-1933014 MS.gene020496:CDS 45.0%
TATCGTGAGCTTGAAAACCG+TGG - chr4.1:1932604-1932623 MS.gene020496:CDS 45.0%
!! ATTTGTTGTGTGACGGGTTG+TGG - chr4.1:1932463-1932482 MS.gene020496:CDS 45.0%
!!! TTAATTTATTTATTTGAAAT+TGG - chr4.1:1932971-1932990 MS.gene020496:CDS 5.0%
AAATGTGGCACAAGGGAACC+AGG - chr4.1:1933010-1933029 MS.gene020496:CDS 50.0%
AATGTGGCACAAGGGAACCA+GGG - chr4.1:1933011-1933030 MS.gene020496:CDS 50.0%
CCAACAACCTAATGCCAACC+AGG - chr4.1:1932097-1932116 MS.gene020496:intron 50.0%
CGAGTCATGTGTTCAACTGC+TGG + chr4.1:1933059-1933078 None:intergenic 50.0%
GAAGCAGTCAAATAGACCCG+TGG + chr4.1:1932129-1932148 None:intergenic 50.0%
GACCATTCCTGGTTGGCATT+AGG + chr4.1:1932107-1932126 None:intergenic 50.0%
GGCATGGAAATGTGGCACAA+GGG - chr4.1:1933003-1933022 MS.gene020496:CDS 50.0%
TGGCATGGAAATGTGGCACA+AGG - chr4.1:1933002-1933021 MS.gene020496:CDS 50.0%
TGGTGTCCATGCATCACCTT+TGG - chr4.1:1932281-1932300 MS.gene020496:intron 50.0%
TGTTCAACTGCTGGAGCTGT+TGG + chr4.1:1933050-1933069 None:intergenic 50.0%
TTGCAGCTTGTGCTGTAAGC+GGG - chr4.1:1932425-1932444 MS.gene020496:intron 50.0%
! CCTGGTTGGCATTAGGTTGT+TGG + chr4.1:1932100-1932119 None:intergenic 50.0%
ATAGACCCGTGGACCATTCC+TGG + chr4.1:1932118-1932137 None:intergenic 55.0%
GTGGCTGGTGGCTGATTGTA+CGG + chr4.1:1932053-1932072 None:intergenic 55.0%
TACAATCAGCCACCAGCCAC+CGG - chr4.1:1932053-1932072 MS.gene020496:CDS 55.0%
TGCAGCTTGTGCTGTAAGCG+GGG - chr4.1:1932426-1932445 MS.gene020496:intron 55.0%
TGCCAACCAGGAATGGTCCA+CGG - chr4.1:1932109-1932128 MS.gene020496:intron 55.0%
!! TGTTGGTAGCCGACGTTCAC+CGG + chr4.1:1932083-1932102 None:intergenic 55.0%
ACCCGTGGACCATTCCTGGT+TGG + chr4.1:1932114-1932133 None:intergenic 60.0%
AGGGAACCAGGGAGTAGCCA+TGG - chr4.1:1933022-1933041 MS.gene020496:CDS 60.0%
GCCAACCAGGAATGGTCCAC+GGG - chr4.1:1932110-1932129 MS.gene020496:intron 60.0%
! ACCGGTTTCCCGGTGAACGT+CGG - chr4.1:1932071-1932090 MS.gene020496:CDS 60.0%
!! GTTGGTAGCCGACGTTCACC+GGG + chr4.1:1932082-1932101 None:intergenic 60.0%
!! TGCTGGAGCTGTTGGTGCCA+TGG + chr4.1:1933042-1933061 None:intergenic 60.0%
!! TTGGTGCCATGGCTACTCCC+TGG + chr4.1:1933031-1933050 None:intergenic 60.0%
GACGTTCACCGGGAAACCGG+TGG + chr4.1:1932072-1932091 None:intergenic 65.0%
GCCGACGTTCACCGGGAAAC+CGG + chr4.1:1932075-1932094 None:intergenic 65.0%
TTCACCGGGAAACCGGTGGC+TGG + chr4.1:1932068-1932087 None:intergenic 65.0%
ACCGGGAAACCGGTGGCTGG+TGG + chr4.1:1932065-1932084 None:intergenic 70.0%
!! GCCACCAGCCACCGGTTTCC+CGG - chr4.1:1932061-1932080 MS.gene020496:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 1932020 1933082 1932020 ID=MS.gene020496
chr4.1 mRNA 1932020 1933082 1932020 ID=MS.gene020496.t1;Parent=MS.gene020496
chr4.1 exon 1932938 1933082 1932938 ID=MS.gene020496.t1.exon1;Parent=MS.gene020496.t1
chr4.1 CDS 1932938 1933082 1932938 ID=cds.MS.gene020496.t1;Parent=MS.gene020496.t1
chr4.1 exon 1932764 1932835 1932764 ID=MS.gene020496.t1.exon2;Parent=MS.gene020496.t1
chr4.1 CDS 1932764 1932835 1932764 ID=cds.MS.gene020496.t1;Parent=MS.gene020496.t1
chr4.1 exon 1932459 1932671 1932459 ID=MS.gene020496.t1.exon3;Parent=MS.gene020496.t1
chr4.1 CDS 1932459 1932671 1932459 ID=cds.MS.gene020496.t1;Parent=MS.gene020496.t1
chr4.1 exon 1932020 1932102 1932020 ID=MS.gene020496.t1.exon4;Parent=MS.gene020496.t1
chr4.1 CDS 1932020 1932102 1932020 ID=cds.MS.gene020496.t1;Parent=MS.gene020496.t1
Gene Sequence

>MS.gene020496

ATGGATCCTCCAAGTTCAAAATTTCAACAACCGTACAATCAGCCACCAGCCACCGGTTTCCCGGTGAACGTCGGCTACCAACAACCTAATGCCAACCAGGAATGGTCCACGGGTCTATTTGACTGCTTCTCGGACTGCAAAACTTGTATGTTATGTTCTAATTACCACTTAATTTGGACCGTTCATTAATTAATTCCTCTTCTAAATTTTAATAATTATATATACTAAATTTCATTTCTTTTTTTAGGTTGCATAACGTTCTGGTGTCCATGCATCACCTTTGGACGAATTGCTGAGATTGTTGACAAGGGATCAACTTGTAAGATATTCATTAATTGGTCTATTAATAATGATGTATAACTATTATATTATACATGTATTAATTATTATATTGTTTGAATTATATTGCAGCTTGTGCTGTAAGCGGGGCTCTATATACATTAATTTGTTGTGTGACGGGTTGTGGTTGTTTATATTCATGCATCTACCGTAACAAAATGAGACAACAATACATGCTCAAAGACACTCCTTGTTGTGATTGCTTAGTTCATTGTTGTTGTGAATCATGTGCATTGTGTCAAGAATATCGTGAGCTTGAAAACCGTGGATTTGACATGGAACTTGGTATGTATATAGTACTATTATTTTGCTCTTCAATAATCTTAATTCTTTTATCTTTTGGTATTCAGTTTTATTCTTTAGTCGACAATTCAATGTTTCTATGGTGCTCTACTTTTAAACGATTCTCTTAAATTTTTTATTTAAGGTCATAGAAATTAAATCACTTTGAAATATATTAGTATTGTACTAGTCAACTTTAATCTTTGAAATTTTCAACGAGAGAGCGATGAGATAAACATGAGTAGAAATAGTTTAAGACACATATTATTTAATTTGTACCATTGTCGCATCTGGATGAGTTTTTTTAATTTTTTTATTACTTGAATCTGTTTAATTTATTTATTTGAAATTGGCTATAGGGTGGCATGGAAATGTGGCACAAGGGAACCAGGGAGTAGCCATGGCACCAACAGCTCCAGCAGTTGAACACATGACTCGTTGA

Protein sequence

>MS.gene020496.t1

MDPPSSKFQQPYNQPPATGFPVNVGYQQPNANQEWSTGLFDCFSDCKTCCITFWCPCITFGRIAEIVDKGSTSCAVSGALYTLICCVTGCGCLYSCIYRNKMRQQYMLKDTPCCDCLVHCCCESCALCQEYRELENRGFDMELGWHGNVAQGNQGVAMAPTAPAVEHMTR