AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29965


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29965.t1 MTR_8g104890 97.647 170 3 1 1 170 1 169 5.38e-123 343
MS.gene29965.t1 MTR_5g017650 60.227 176 50 3 1 156 1 176 4.81e-68 205
MS.gene29965.t1 MTR_8g104870 63.190 163 42 3 12 156 11 173 1.40e-65 199
MS.gene29965.t1 MTR_4g086330 50.602 166 71 2 14 170 34 197 2.85e-53 168
MS.gene29965.t1 MTR_8g104830 50.714 140 66 3 30 167 4 142 6.17e-43 140
MS.gene29965.t1 MTR_4g086320 56.667 120 51 1 25 144 42 160 1.45e-42 139
MS.gene29965.t1 MTR_2g101660 33.728 169 109 1 5 170 3 171 4.68e-31 110
MS.gene29965.t1 MTR_6g084940 43.066 137 74 3 34 170 17 149 1.39e-29 106
MS.gene29965.t1 MTR_1g075550 53.509 114 52 1 34 147 11 123 4.45e-26 97.1
MS.gene29965.t1 MTR_5g008240 39.416 137 68 4 21 142 27 163 4.01e-24 93.2
MS.gene29965.t1 MTR_5g008240 37.956 137 70 3 21 142 69 205 7.79e-24 93.6
MS.gene29965.t1 MTR_5g008240 37.956 137 70 3 21 142 93 229 9.69e-24 94.0
MS.gene29965.t1 MTR_2g101660 36.585 123 75 1 51 170 1 123 1.04e-23 90.5
MS.gene29965.t1 MTR_7g044840 31.655 139 65 3 28 137 53 190 5.10e-19 81.3
MS.gene29965.t1 MTR_0172s0050 32.414 145 64 4 29 140 57 200 1.02e-15 72.4
MS.gene29965.t1 MTR_3g408350 27.972 143 64 2 29 137 53 190 9.55e-15 69.7
MS.gene29965.t1 MTR_5g008240 40.741 108 49 4 50 142 38 145 1.34e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29965.t1 AT1G14870 64.626 147 49 3 26 170 7 152 4.68e-63 191
MS.gene29965.t1 AT5G35525 64.029 139 48 2 29 166 10 147 9.47e-60 183
MS.gene29965.t1 AT1G14870 68.067 119 38 0 26 144 7 125 2.07e-57 176
MS.gene29965.t1 AT1G14880 59.864 147 56 3 26 170 6 151 3.62e-55 171
MS.gene29965.t1 AT1G49030 48.428 159 71 4 5 160 66 216 1.10e-45 149
MS.gene29965.t1 AT1G68610 56.934 137 56 2 33 166 17 153 5.91e-45 145
MS.gene29965.t1 AT3G18470 50.388 129 59 1 32 160 2 125 1.06e-42 139
MS.gene29965.t1 AT3G18460 50.685 146 63 4 18 162 42 179 1.24e-42 140
MS.gene29965.t1 AT3G18450 46.358 151 74 3 10 160 33 176 2.10e-42 140
MS.gene29965.t1 AT1G58320 50.413 121 56 3 34 153 16 133 1.15e-36 124
MS.gene29965.t1 AT1G52200 39.535 172 94 4 9 170 19 190 6.11e-34 118
MS.gene29965.t1 AT1G52200 39.583 144 79 2 9 144 19 162 2.17e-28 104
MS.gene29965.t1 AT1G68630 38.298 141 82 2 26 162 16 155 3.10e-28 103
MS.gene29965.t1 AT2G40935 34.028 144 73 4 22 144 34 176 4.90e-22 88.2
MS.gene29965.t1 AT2G40935 34.028 144 73 4 22 144 34 176 4.90e-22 88.2
MS.gene29965.t1 AT2G40935 34.028 144 73 4 22 144 34 176 4.90e-22 88.2
MS.gene29965.t1 AT2G40935 34.028 144 73 4 22 144 34 176 4.90e-22 88.2
MS.gene29965.t1 AT2G40935 34.028 144 73 4 22 144 34 176 4.90e-22 88.2
MS.gene29965.t1 AT2G45010 33.103 145 66 4 28 142 9 152 9.41e-20 82.4
MS.gene29965.t1 AT2G45010 33.103 145 66 4 28 142 55 198 1.27e-19 82.8
MS.gene29965.t1 AT5G51400 30.921 152 73 5 19 140 47 196 1.41e-17 77.4
MS.gene29965.t1 AT2G40935 31.250 144 66 5 22 144 34 165 5.73e-16 72.0
MS.gene29965.t1 AT2G40935 35.345 116 53 4 50 144 38 152 1.32e-14 68.2
MS.gene29965.t1 AT2G40935 35.345 116 53 4 50 144 38 152 1.32e-14 68.2
MS.gene29965.t1 AT2G40935 35.345 116 53 4 50 144 38 152 1.32e-14 68.2

Find 48 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCATTCCTGGTTGGCATT+AGG 0.310976 4.3:+1623883 None:intergenic
GCCACCAGCCACCGGTTTCC+CGG 0.332845 4.3:-1623926 MS.gene29965:CDS
CCTGGTTGGCATTAGGTTGT+TGG 0.349397 4.3:+1623890 None:intergenic
ATAGACCCGTGGACCATTCC+TGG 0.365914 4.3:+1623872 None:intergenic
CGTGGATTTGACATGGAACT+TGG 0.398558 4.3:-1623365 MS.gene29965:intron
GCCTACGTTCACCGGGAAAC+CGG 0.412709 4.3:+1623915 None:intergenic
ATTTGTTGTGTGACGGGTTG+TGG 0.418139 4.3:-1623524 MS.gene29965:CDS
TGTTGGTGGCCTACGTTCAC+CGG 0.423504 4.3:+1623907 None:intergenic
TGGTGTCCATGCATCACCTT+TGG 0.426284 4.3:-1623706 MS.gene29965:CDS
TTGCAGCTTGTGCTGTAAGC+GGG 0.457897 4.3:-1623562 MS.gene29965:intron
TACATTAATTTGTTGTGTGA+CGG 0.462544 4.3:-1623531 MS.gene29965:CDS
TGCTGGAGCTGTTGGTGCCA+TGG 0.464678 4.3:+1622948 None:intergenic
GTGGCTGATTGTACGGTTGT+TGG 0.466033 4.3:+1623944 None:intergenic
TGTTCAACTGCTGGAGCTGT+TGG 0.477230 4.3:+1622940 None:intergenic
CGAGTCATGTGTTCAACTGC+TGG 0.481564 4.3:+1622931 None:intergenic
ACATTAATTTGTTGTGTGAC+GGG 0.489462 4.3:-1623530 MS.gene29965:CDS
AAATGTGGCACAAGGGAACC+AGG 0.489547 4.3:-1622977 MS.gene29965:CDS
GGTTGGCATTAGGTTGTTGG+TGG 0.517094 4.3:+1623893 None:intergenic
GTGGCTGGTGGCTGATTGTA+CGG 0.517478 4.3:+1623937 None:intergenic
TTGGTGCCATGGCTACTCCC+TGG 0.530182 4.3:+1622959 None:intergenic
AACCTAATGCCAACCAGGAA+TGG 0.530457 4.3:-1623885 MS.gene29965:CDS
GCCAACCAGGAATGGTCCAC+GGG 0.546652 4.3:-1623877 MS.gene29965:CDS
GTTGGTGGCCTACGTTCACC+GGG 0.550874 4.3:+1623908 None:intergenic
TTCACCGGGAAACCGGTGGC+TGG 0.551343 4.3:+1623922 None:intergenic
ACCGGGAAACCGGTGGCTGG+TGG 0.560600 4.3:+1623925 None:intergenic
TACAATCAGCCACCAGCCAC+CGG 0.567328 4.3:-1623934 MS.gene29965:CDS
ATTGCAGCTTGTGCTGTAAG+CGG 0.567979 4.3:-1623563 MS.gene29965:intron
AAATTGGCTATAGGGTGGCA+TGG 0.569669 4.3:-1623000 MS.gene29965:intron
CCAACAACCTAATGCCAACC+AGG 0.574274 4.3:-1623890 MS.gene29965:CDS
TGGCATGGAAATGTGGCACA+AGG 0.574561 4.3:-1622985 MS.gene29965:CDS
TGCCAACCAGGAATGGTCCA+CGG 0.582618 4.3:-1623878 MS.gene29965:CDS
TGCAGCTTGTGCTGTAAGCG+GGG 0.590429 4.3:-1623561 MS.gene29965:intron
AATCTCAGCAATTCGTCCAA+AGG 0.597233 4.3:+1623690 None:intergenic
ATTCGTCCAAAGGTGATGCA+TGG 0.598061 4.3:+1623700 None:intergenic
TGAAAACCGTGGATTTGACA+TGG 0.599587 4.3:-1623372 MS.gene29965:CDS
ACCCGTGGACCATTCCTGGT+TGG 0.600890 4.3:+1623876 None:intergenic
AATTGCTGAGATTGTTGACA+AGG 0.603304 4.3:-1623680 MS.gene29965:CDS
TATAGGGTGGCATGGAAATG+TGG 0.605383 4.3:-1622992 MS.gene29965:intron
AGGGAACCAGGGAGTAGCCA+TGG 0.614002 4.3:-1622965 MS.gene29965:CDS
CAAGTTCCATGTCAAATCCA+CGG 0.615456 4.3:+1623366 None:intergenic
TACGTTCACCGGGAAACCGG+TGG 0.615540 4.3:+1623918 None:intergenic
AATGTGGCACAAGGGAACCA+GGG 0.650557 4.3:-1622976 MS.gene29965:CDS
GGCATGGAAATGTGGCACAA+GGG 0.652763 4.3:-1622984 MS.gene29965:CDS
TGAACTAAGCAATCACAACA+AGG 0.663046 4.3:+1623440 None:intergenic
ATTGCTGAGATTGTTGACAA+GGG 0.678367 4.3:-1623679 MS.gene29965:CDS
ACCGGTTTCCCGGTGAACGT+AGG 0.680377 4.3:-1623916 MS.gene29965:CDS
TATCGTGAGCTTGAAAACCG+TGG 0.692905 4.3:-1623383 MS.gene29965:CDS
GAAGCAGTCAAATAGACCCG+TGG 0.701710 4.3:+1623861 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATTAAAATTTAGAAG+AGG + chr4.3:1623125-1623144 None:intergenic 10.0%
!! TAGTTATATATCATTATTAA+TGG + chr4.3:1623270-1623289 None:intergenic 10.0%
!!! TAAATTTCATTTCATTTTTT+AGG - chr4.3:1623152-1623171 MS.gene29965:intron 10.0%
!!! TCTCTTAAATTTTTTATTTA+AGG - chr4.3:1623671-1623690 MS.gene29965:CDS 10.0%
!!! ATCTTAATTCTTTTATCTTT+TGG - chr4.3:1623586-1623605 MS.gene29965:intron 15.0%
!! CTTGTAAGATATTCATTAAT+TGG - chr4.3:1623243-1623262 MS.gene29965:intron 20.0%
!! TTAGAAGAGGAATTAATTAA+TGG + chr4.3:1623112-1623131 None:intergenic 20.0%
!!! ATTTATTTGAAATTGGCTAT+AGG - chr4.3:1623885-1623904 MS.gene29965:CDS 20.0%
!!! TTTATTTGAAATTGGCTATA+GGG - chr4.3:1623886-1623905 MS.gene29965:CDS 20.0%
! AAGAGGAATTAATTAATGGA+CGG + chr4.3:1623108-1623127 None:intergenic 25.0%
! GTTCTAATTACCACTTAATT+TGG - chr4.3:1623081-1623100 MS.gene29965:intron 25.0%
!! ATTGTTGTCTCATTTTGTTA+CGG + chr4.3:1623417-1623436 None:intergenic 25.0%
!!! TACATTAATTTGTTGTGTGA+CGG - chr4.3:1623363-1623382 MS.gene29965:intron 25.0%
! TAACATACAAGTTTTGCAGT+CGG + chr4.3:1623060-1623079 None:intergenic 30.0%
! TCGACAATTCAATGTTTCTA+TGG - chr4.3:1623629-1623648 MS.gene29965:intron 30.0%
!! ACATTAATTTGTTGTGTGAC+GGG - chr4.3:1623364-1623383 MS.gene29965:intron 30.0%
AATTGCTGAGATTGTTGACA+AGG - chr4.3:1623214-1623233 MS.gene29965:intron 35.0%
ATTGCTGAGATTGTTGACAA+GGG - chr4.3:1623215-1623234 MS.gene29965:intron 35.0%
TGAACTAAGCAATCACAACA+AGG + chr4.3:1623457-1623476 None:intergenic 35.0%
! ATTTGAAATTGGCTATAGGG+TGG - chr4.3:1623889-1623908 MS.gene29965:CDS 35.0%
! GGTTGTTGGAATTTTGAACT+TGG + chr4.3:1622939-1622958 None:intergenic 35.0%
! TGTTGGAATTTTGAACTTGG+AGG + chr4.3:1622936-1622955 None:intergenic 35.0%
!!! TTTTAGGTTGCATAACGTTC+TGG - chr4.3:1623168-1623187 MS.gene29965:intron 35.0%
AATCTCAGCAATTCGTCCAA+AGG + chr4.3:1623207-1623226 None:intergenic 40.0%
AATGGACGGTCCAAATTAAG+TGG + chr4.3:1623094-1623113 None:intergenic 40.0%
ATTTGTACCATTGTCGCATC+TGG - chr4.3:1623819-1623838 MS.gene29965:intron 40.0%
CAAGTTCCATGTCAAATCCA+CGG + chr4.3:1623531-1623550 None:intergenic 40.0%
TGAAAACCGTGGATTTGACA+TGG - chr4.3:1623522-1623541 MS.gene29965:CDS 40.0%
AAATTGGCTATAGGGTGGCA+TGG - chr4.3:1623894-1623913 MS.gene29965:CDS 45.0%
AACCTAATGCCAACCAGGAA+TGG - chr4.3:1623009-1623028 MS.gene29965:intron 45.0%
AACTCATCCAGATGCGACAA+TGG + chr4.3:1623829-1623848 None:intergenic 45.0%
ATTCGTCCAAAGGTGATGCA+TGG + chr4.3:1623197-1623216 None:intergenic 45.0%
ATTGCAGCTTGTGCTGTAAG+CGG - chr4.3:1623331-1623350 MS.gene29965:intron 45.0%
CGTGGATTTGACATGGAACT+TGG - chr4.3:1623529-1623548 MS.gene29965:CDS 45.0%
TATAGGGTGGCATGGAAATG+TGG - chr4.3:1623902-1623921 MS.gene29965:CDS 45.0%
TATCGTGAGCTTGAAAACCG+TGG - chr4.3:1623511-1623530 MS.gene29965:CDS 45.0%
!! ATTTGTTGTGTGACGGGTTG+TGG - chr4.3:1623370-1623389 MS.gene29965:CDS 45.0%
!!! TTAATTTATTTATTTGAAAT+TGG - chr4.3:1623878-1623897 MS.gene29965:CDS 5.0%
AAATGTGGCACAAGGGAACC+AGG - chr4.3:1623917-1623936 MS.gene29965:CDS 50.0%
AATGTGGCACAAGGGAACCA+GGG - chr4.3:1623918-1623937 MS.gene29965:CDS 50.0%
CCAACAACCTAATGCCAACC+AGG - chr4.3:1623004-1623023 MS.gene29965:intron 50.0%
CGAGTCATGTGTTCAACTGC+TGG + chr4.3:1623966-1623985 None:intergenic 50.0%
GAAGCAGTCAAATAGACCCG+TGG + chr4.3:1623036-1623055 None:intergenic 50.0%
GACCATTCCTGGTTGGCATT+AGG + chr4.3:1623014-1623033 None:intergenic 50.0%
GGCATGGAAATGTGGCACAA+GGG - chr4.3:1623910-1623929 MS.gene29965:CDS 50.0%
GTGGCTGATTGTACGGTTGT+TGG + chr4.3:1622953-1622972 None:intergenic 50.0%
TGGCATGGAAATGTGGCACA+AGG - chr4.3:1623909-1623928 MS.gene29965:CDS 50.0%
TGGTGTCCATGCATCACCTT+TGG - chr4.3:1623188-1623207 MS.gene29965:intron 50.0%
TGTTCAACTGCTGGAGCTGT+TGG + chr4.3:1623957-1623976 None:intergenic 50.0%
TTGCAGCTTGTGCTGTAAGC+GGG - chr4.3:1623332-1623351 MS.gene29965:intron 50.0%
! CCTGGTTGGCATTAGGTTGT+TGG + chr4.3:1623007-1623026 None:intergenic 50.0%
! GGTTGGCATTAGGTTGTTGG+TGG + chr4.3:1623004-1623023 None:intergenic 50.0%
ATAGACCCGTGGACCATTCC+TGG + chr4.3:1623025-1623044 None:intergenic 55.0%
GTGGCTGGTGGCTGATTGTA+CGG + chr4.3:1622960-1622979 None:intergenic 55.0%
TACAATCAGCCACCAGCCAC+CGG - chr4.3:1622960-1622979 MS.gene29965:CDS 55.0%
TGCAGCTTGTGCTGTAAGCG+GGG - chr4.3:1623333-1623352 MS.gene29965:intron 55.0%
TGCCAACCAGGAATGGTCCA+CGG - chr4.3:1623016-1623035 MS.gene29965:intron 55.0%
!! TGTTGGTGGCCTACGTTCAC+CGG + chr4.3:1622990-1623009 None:intergenic 55.0%
ACCCGTGGACCATTCCTGGT+TGG + chr4.3:1623021-1623040 None:intergenic 60.0%
AGGGAACCAGGGAGTAGCCA+TGG - chr4.3:1623929-1623948 MS.gene29965:CDS 60.0%
GCCAACCAGGAATGGTCCAC+GGG - chr4.3:1623017-1623036 MS.gene29965:intron 60.0%
GCCTACGTTCACCGGGAAAC+CGG + chr4.3:1622982-1623001 None:intergenic 60.0%
TACGTTCACCGGGAAACCGG+TGG + chr4.3:1622979-1622998 None:intergenic 60.0%
! ACCGGTTTCCCGGTGAACGT+AGG - chr4.3:1622978-1622997 MS.gene29965:CDS 60.0%
!! GTTGGTGGCCTACGTTCACC+GGG + chr4.3:1622989-1623008 None:intergenic 60.0%
!! TGCTGGAGCTGTTGGTGCCA+TGG + chr4.3:1623949-1623968 None:intergenic 60.0%
!! TTGGTGCCATGGCTACTCCC+TGG + chr4.3:1623938-1623957 None:intergenic 60.0%
TTCACCGGGAAACCGGTGGC+TGG + chr4.3:1622975-1622994 None:intergenic 65.0%
ACCGGGAAACCGGTGGCTGG+TGG + chr4.3:1622972-1622991 None:intergenic 70.0%
!! GCCACCAGCCACCGGTTTCC+CGG - chr4.3:1622968-1622987 MS.gene29965:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 1622927 1623989 1622927 ID=MS.gene29965
chr4.3 mRNA 1622927 1623989 1622927 ID=MS.gene29965.t1;Parent=MS.gene29965
chr4.3 exon 1623845 1623989 1623845 ID=MS.gene29965.t1.exon1;Parent=MS.gene29965.t1
chr4.3 CDS 1623845 1623989 1623845 ID=cds.MS.gene29965.t1;Parent=MS.gene29965.t1
chr4.3 exon 1623671 1623742 1623671 ID=MS.gene29965.t1.exon2;Parent=MS.gene29965.t1
chr4.3 CDS 1623671 1623742 1623671 ID=cds.MS.gene29965.t1;Parent=MS.gene29965.t1
chr4.3 exon 1623366 1623578 1623366 ID=MS.gene29965.t1.exon3;Parent=MS.gene29965.t1
chr4.3 CDS 1623366 1623578 1623366 ID=cds.MS.gene29965.t1;Parent=MS.gene29965.t1
chr4.3 exon 1622927 1623009 1622927 ID=MS.gene29965.t1.exon4;Parent=MS.gene29965.t1
chr4.3 CDS 1622927 1623009 1622927 ID=cds.MS.gene29965.t1;Parent=MS.gene29965.t1
Gene Sequence

>MS.gene29965

ATGAATCCTCCAAGTTCAAAATTCCAACAACCGTACAATCAGCCACCAGCCACCGGTTTCCCGGTGAACGTAGGCCACCAACAACCTAATGCCAACCAGGAATGGTCCACGGGTCTATTTGACTGCTTCTCCGACTGCAAAACTTGTATGTTATGTTCTAATTACCACTTAATTTGGACCGTCCATTAATTAATTCCTCTTCTAAATTTTAATAATTATATATACTAAATTTCATTTCATTTTTTAGGTTGCATAACGTTCTGGTGTCCATGCATCACCTTTGGACGAATTGCTGAGATTGTTGACAAGGGATCAACTTGTAAGATATTCATTAATTGGTCCATTAATAATGATATATAACTATTATATTATACATGTATTAATTATTATATTGTTTGAATTATATTGCAGCTTGTGCTGTAAGCGGGGCTCTATATACATTAATTTGTTGTGTGACGGGTTGTGGTTGTTTATACTCGTGCATCTACCGTAACAAAATGAGACAACAATACATGCTCAAAGACACTCCTTGTTGTGATTGCTTAGTTCATTGTTGTTGTGAATCATGTGCATTATGTCAAGAATATCGTGAGCTTGAAAACCGTGGATTTGACATGGAACTTGGTATGTATATAGTACTATTATTTTGCTCTTCAATAATCTTAATTCTTTTATCTTTTGGTATTCAGTTTTATTCTTTAGTCGACAATTCAATGTTTCTATGGTGCTCTACTTTTAAACGATTCTCTTAAATTTTTTATTTAAGGTCATAGAAATTAAATCACTTTGAAATATATTAGTATTGTACTAGTCAACTTTAATCTTTGAAATTTTCAACGAGAGAGCGATGAGATAAACATGAGTAGAAATAGTTTAAGACACATATTATTTAATTTGTACCATTGTCGCATCTGGATGAGTTTTTTTAATTTTTTTATTACTTGAATCTGTTTAATTTATTTATTTGAAATTGGCTATAGGGTGGCATGGAAATGTGGCACAAGGGAACCAGGGAGTAGCCATGGCACCAACAGCTCCAGCAGTTGAACACATGACTCGTTGA

Protein sequence

>MS.gene29965.t1

MNPPSSKFQQPYNQPPATGFPVNVGHQQPNANQEWSTGLFDCFSDCKTCCITFWCPCITFGRIAEIVDKGSTSCAVSGALYTLICCVTGCGCLYSCIYRNKMRQQYMLKDTPCCDCLVHCCCESCALCQEYRELENRGFDMELGWHGNVAQGNQGVAMAPTAPAVEHMTR