AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021127

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021127.t1 MTR_4g063975 98.450 258 4 0 1 258 1 258 0.0 525
MS.gene021127.t1 MTR_2g097030 87.209 258 33 0 1 258 3 260 7.05e-175 482
MS.gene021127.t1 MTR_5g079950 82.661 248 43 0 11 258 12 259 2.63e-161 447
MS.gene021127.t1 MTR_7g061720 82.171 258 45 1 2 258 1 258 5.96e-161 447
MS.gene021127.t1 MTR_5g041700 66.800 250 81 1 8 257 1 248 1.62e-124 354
MS.gene021127.t1 MTR_4g081950 65.217 253 81 3 2 253 34 280 1.34e-122 350
MS.gene021127.t1 MTR_3g094060 64.314 255 86 3 5 256 1 253 5.56e-122 348
MS.gene021127.t1 MTR_3g094040 64.173 254 86 3 5 256 3 253 7.22e-122 347
MS.gene021127.t1 MTR_5g075320 63.780 254 87 3 5 256 1 251 4.22e-120 343
MS.gene021127.t1 MTR_3g435430 70.222 225 65 1 32 256 25 247 9.52e-120 342
MS.gene021127.t1 MTR_3g466760 63.281 256 92 1 1 256 7 260 3.80e-117 336
MS.gene021127.t1 MTR_3g466790 63.424 257 90 3 1 256 1 254 2.03e-116 334
MS.gene021127.t1 MTR_7g011060 63.265 245 80 2 12 256 6 240 9.97e-115 329
MS.gene021127.t1 MTR_7g111010 57.312 253 100 4 2 251 1 248 7.82e-107 310
MS.gene021127.t1 MTR_1g085460 61.157 242 86 4 16 254 18 254 1.30e-106 309
MS.gene021127.t1 MTR_6g012520 62.698 252 84 2 5 256 1 242 3.74e-105 305
MS.gene021127.t1 MTR_1g026020 57.422 256 103 3 1 254 1 252 7.24e-105 305
MS.gene021127.t1 MTR_7g110990 62.780 223 79 3 32 251 12 233 2.22e-103 300
MS.gene021127.t1 MTR_4g124340 59.471 227 89 2 30 255 59 283 7.91e-103 300
MS.gene021127.t1 MTR_8g464350 55.061 247 106 2 5 251 7 248 1.70e-100 293
MS.gene021127.t1 MTR_7g013150 55.738 244 105 2 11 254 10 250 5.15e-98 287
MS.gene021127.t1 MTR_8g058990 57.207 222 93 1 32 253 30 249 7.77e-95 279
MS.gene021127.t1 MTR_7g013140 57.399 223 93 1 32 254 28 248 4.86e-94 277
MS.gene021127.t1 MTR_7g013120 52.653 245 111 3 11 254 10 250 1.36e-93 276
MS.gene021127.t1 MTR_5g011210 50.394 254 123 3 6 257 11 263 1.59e-92 273
MS.gene021127.t1 MTR_4g102450 50.394 254 123 3 6 257 10 262 1.16e-91 271
MS.gene021127.t1 MTR_0040s0140 47.287 258 131 2 1 256 2 256 1.17e-88 263
MS.gene021127.t1 MTR_5g076950 55.310 226 100 1 31 256 40 264 7.48e-88 262
MS.gene021127.t1 MTR_2g090255 46.341 246 131 1 12 256 7 252 5.53e-83 249
MS.gene021127.t1 MTR_3g064510 52.340 235 103 5 31 257 52 285 1.41e-79 241
MS.gene021127.t1 MTR_0008s0120 59.091 110 45 0 144 253 86 195 3.96e-42 143
MS.gene021127.t1 MTR_0008s0120 56.471 85 33 2 5 88 7 88 2.92e-24 96.7
MS.gene021127.t1 MTR_6g012500 42.857 175 84 4 98 256 106 280 1.93e-40 141
MS.gene021127.t1 MTR_2g090270 30.041 243 144 9 24 254 27 255 5.82e-25 100
MS.gene021127.t1 MTR_2g090265 28.947 266 160 11 4 254 2 253 1.93e-23 95.9
MS.gene021127.t1 MTR_0122s0020 31.780 236 135 11 31 254 40 261 9.38e-23 94.4
MS.gene021127.t1 MTR_4g028640 29.237 236 132 11 32 251 42 258 4.14e-18 81.6
MS.gene021127.t1 MTR_0118s0070 28.571 231 135 12 37 251 53 269 1.86e-16 77.4
MS.gene021127.t1 MTR_0809s0010 28.512 242 137 13 27 251 42 264 2.42e-15 73.9
MS.gene021127.t1 MTR_0122s0020 33.838 198 97 12 21 198 21 204 7.79e-15 71.6
MS.gene021127.t1 MTR_5g013440 25.991 227 151 9 35 254 32 248 1.84e-13 68.6
MS.gene021127.t1 MTR_4g099370 25.751 233 152 10 28 251 24 244 3.81e-13 67.8
MS.gene021127.t1 MTR_1g085365 53.571 56 26 0 201 256 4 59 4.19e-13 63.2
MS.gene021127.t1 MTR_4g075790 28.378 222 124 9 43 248 32 234 4.79e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021127.t1 AT2G39700 82.879 257 44 0 2 258 1 257 3.97e-164 454
MS.gene021127.t1 AT2G28950 82.490 257 45 0 2 258 1 257 4.14e-156 434
MS.gene021127.t1 AT2G37640 79.447 253 52 0 6 258 10 262 3.81e-154 429
MS.gene021127.t1 AT3G55500 78.346 254 55 0 5 258 7 260 5.09e-154 429
MS.gene021127.t1 AT5G02260 74.419 258 66 0 1 258 1 258 8.36e-152 424
MS.gene021127.t1 AT2G37640 82.381 210 37 0 49 258 28 237 1.97e-132 374
MS.gene021127.t1 AT1G69530 66.798 253 81 2 5 257 1 250 6.81e-125 355
MS.gene021127.t1 AT1G69530 66.798 253 81 2 5 257 1 250 6.81e-125 355
MS.gene021127.t1 AT1G69530 66.403 253 82 2 5 257 1 250 2.60e-123 352
MS.gene021127.t1 AT2G03090 66.935 248 79 2 11 257 8 253 4.62e-122 348
MS.gene021127.t1 AT5G56320 64.844 256 83 3 5 257 1 252 1.07e-120 345
MS.gene021127.t1 AT5G56320 64.844 256 83 3 5 257 1 252 1.07e-120 345
MS.gene021127.t1 AT5G56320 64.844 256 83 3 5 257 1 252 6.84e-120 344
MS.gene021127.t1 AT1G26770 66.403 253 81 2 5 257 1 249 9.40e-118 337
MS.gene021127.t1 AT1G26770 66.142 254 82 2 4 257 10 259 1.29e-117 337
MS.gene021127.t1 AT5G05290 62.109 256 93 3 2 256 3 255 4.97e-115 330
MS.gene021127.t1 AT2G40610 62.698 252 88 3 6 256 7 253 3.22e-114 328
MS.gene021127.t1 AT3G29030 65.929 226 68 2 32 257 38 254 3.93e-110 318
MS.gene021127.t1 AT1G69530 61.660 253 64 3 5 257 1 220 4.74e-109 314
MS.gene021127.t1 AT1G69530 61.265 253 65 3 5 257 1 220 1.26e-107 311
MS.gene021127.t1 AT4G01630 61.066 244 87 5 11 251 11 249 5.74e-106 307
MS.gene021127.t1 AT1G20190 61.538 221 82 2 34 252 28 247 1.56e-97 286
MS.gene021127.t1 AT5G39290 50.000 278 111 5 1 254 3 276 1.30e-95 282
MS.gene021127.t1 AT5G39270 50.000 278 111 4 1 254 3 276 4.23e-95 281
MS.gene021127.t1 AT1G62980 52.800 250 116 2 6 254 5 253 9.64e-93 274
MS.gene021127.t1 AT5G39280 55.157 223 96 2 32 254 54 272 1.31e-89 266
MS.gene021127.t1 AT1G12560 51.807 249 116 4 11 257 16 262 1.86e-89 266
MS.gene021127.t1 AT5G39300 47.636 275 119 5 1 254 3 273 7.35e-89 265
MS.gene021127.t1 AT5G39310 54.911 224 96 3 32 254 90 309 2.30e-87 262
MS.gene021127.t1 AT3G03220 53.509 228 105 1 31 258 39 265 3.89e-86 257
MS.gene021127.t1 AT4G38210 45.565 248 133 1 12 257 8 255 1.14e-80 243
MS.gene021127.t1 AT5G39260 47.191 267 120 6 1 255 3 260 1.81e-76 233
MS.gene021127.t1 AT1G20190 57.979 188 76 2 67 252 2 188 2.90e-76 229
MS.gene021127.t1 AT3G15370 46.512 258 123 7 1 254 1 247 9.12e-76 231
MS.gene021127.t1 AT3G15370 41.667 288 123 8 1 254 1 277 3.30e-69 215
MS.gene021127.t1 AT4G28250 30.705 241 138 11 28 254 32 257 9.76e-24 97.1
MS.gene021127.t1 AT2G20750 29.114 237 144 9 31 254 39 264 7.88e-22 92.0
MS.gene021127.t1 AT1G65680 30.126 239 138 11 28 251 43 267 4.08e-20 87.4
MS.gene021127.t1 AT4G28250 29.583 240 136 11 28 254 32 251 5.68e-20 86.7
MS.gene021127.t1 AT2G20750 32.386 176 97 7 31 195 39 203 3.50e-16 75.5
MS.gene021127.t1 AT4G28250 32.240 183 95 8 28 200 32 195 1.16e-15 73.9
MS.gene021127.t1 AT4G28250 32.584 178 91 8 28 195 32 190 7.20e-15 71.6
MS.gene021127.t1 AT3G60570 23.774 265 172 9 9 258 10 259 3.02e-13 68.2
MS.gene021127.t1 AT4G17030 25.000 212 138 7 3 209 4 199 5.62e-13 67.4
MS.gene021127.t1 AT3G45970 25.000 212 137 9 51 251 15 215 5.68e-12 64.3

Find 70 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCAATGTAGAGCATTT+TGG 0.215132 4.4:-42003087 None:intergenic
TCACTTGGTTGAGCAAAATT+AGG 0.253485 4.4:-42003674 None:intergenic
GCTCCTCCTAGTTGGCAATT+TGG 0.267981 4.4:+42005651 MS.gene021127:CDS
GACAAAGAGTGGTGTCATTC+TGG 0.278401 4.4:+42003611 MS.gene021127:CDS
GATGCTCATGCTACTTTCTA+TGG 0.312345 4.4:+42003179 MS.gene021127:CDS
TTTGCTCAACCAAGTGATAA+TGG 0.336925 4.4:+42003680 MS.gene021127:CDS
TATGGTGGATCTGATGCTTC+TGG 0.339901 4.4:+42003197 MS.gene021127:CDS
GCCAGTTCTGACCCCAGTTT+CGG 0.341334 4.4:-42005545 None:intergenic
GGATGCCACTGAGCCGAAAC+TGG 0.348922 4.4:+42005532 MS.gene021127:CDS
ATTGTTGAAGCTAGAATTCC+TGG 0.372131 4.4:+42003131 MS.gene021127:CDS
CAATTTGGTCAAACTTTCAC+TGG 0.389947 4.4:+42005666 MS.gene021127:CDS
ACTAACCTGCGGTAAGCAAC+AGG 0.390868 4.4:-42003788 None:intergenic
CAGTGGCATCCACTCGGTTC+GGG 0.402414 4.4:-42005520 None:intergenic
GCAATCCAACGCGGTGTTAG+TGG 0.422657 4.4:+42005566 MS.gene021127:CDS
TCAGTGGCATCCACTCGGTT+CGG 0.423453 4.4:-42005521 None:intergenic
ACCGTAGATCTTCAACATCT+TGG 0.445386 4.4:+42005622 MS.gene021127:CDS
GCCGAAACTGGGGTCAGAAC+TGG 0.448238 4.4:+42005544 MS.gene021127:CDS
CAACGCGGTGTTAGTGGGCC+AGG 0.450660 4.4:+42005572 MS.gene021127:CDS
CACCAACCACCATTATCACT+TGG 0.453463 4.4:-42003689 None:intergenic
CGGATTCCGTTACTTTAACC+TGG 0.456362 4.4:+42005440 MS.gene021127:CDS
GTTTGACCAAATTGCCAACT+AGG 0.460335 4.4:-42005657 None:intergenic
AGAATTCCTGGTGTTTACTC+TGG 0.487043 4.4:+42003143 MS.gene021127:CDS
GGAACATTGCTCCTCCTAGT+TGG 0.491170 4.4:+42005643 MS.gene021127:CDS
GTAGCAGTAATGAAAATTGA+AGG 0.495390 4.4:-42003638 None:intergenic
TCTGATGCTTCTGGCACCAT+GGG 0.503667 4.4:+42003206 MS.gene021127:CDS
TTAGGAGGAGCATGTGGGTA+CGG 0.517583 4.4:+42003482 MS.gene021127:intron
GTGTTTACTCTGGTGGTCAA+TGG 0.519210 4.4:+42003153 MS.gene021127:CDS
ATTCCTGGTGTTTACTCTGG+TGG 0.519949 4.4:+42003146 MS.gene021127:CDS
TTGTCCCTGTTGCTTACCGC+AGG 0.522475 4.4:+42003783 MS.gene021127:CDS
TCAGCAATCTTAAGAAACAT+AGG 0.524078 4.4:-42003746 None:intergenic
AATTTGTACAGTCAAGGTTA+CGG 0.526954 4.4:+42003506 MS.gene021127:CDS
ATTAGCAACGTAGCAGGTGC+TGG 0.528629 4.4:+42005468 MS.gene021127:CDS
ATCTGATGCTTCTGGCACCA+TGG 0.529188 4.4:+42003205 MS.gene021127:CDS
GTGCTAATTAGCAACGTAGC+AGG 0.532373 4.4:+42005462 MS.gene021127:CDS
CTACGTTGCTAATTAGCACC+AGG 0.535801 4.4:-42005458 None:intergenic
AAAGTAACGGAATCCGTTCA+CGG 0.539938 4.4:-42005433 None:intergenic
TCCAAGATGTTGAAGATCTA+CGG 0.544252 4.4:-42005623 None:intergenic
GATGCCACTGAGCCGAAACT+GGG 0.544292 4.4:+42005533 MS.gene021127:CDS
CAATCCAACGCGGTGTTAGT+GGG 0.545691 4.4:+42005567 MS.gene021127:CDS
GGAATAAGATTCACCGTGAA+CGG 0.547347 4.4:+42005420 MS.gene021127:CDS
ATCGTGCGCGCCTACGTGAA+AGG 0.554876 4.4:+42005495 MS.gene021127:CDS
TACGGAAATTTGTACAGTCA+AGG 0.556701 4.4:+42003500 MS.gene021127:CDS
ATTCCACCACGCTTTCTGCA+TGG 0.558295 4.4:-42005402 None:intergenic
AAGATTGCTGAGTATCGTGC+TGG 0.570660 4.4:+42003758 MS.gene021127:CDS
AGACACGAAAGGACAAAGCC+TGG 0.571645 4.4:-42005590 None:intergenic
AGCACTGCTTTGTTCAACAA+TGG 0.578791 4.4:+42003548 MS.gene021127:CDS
GGTTCGGGTTCCTTTCACGT+AGG 0.581009 4.4:-42005505 None:intergenic
TCGGCTCAGTGGCATCCACT+CGG 0.585195 4.4:-42005526 None:intergenic
TTCAACAATGGTTTAAGCTG+TGG 0.599006 4.4:+42003560 MS.gene021127:CDS
CTTGGTTGAGCAAAATTAGG+AGG 0.603163 4.4:-42003671 None:intergenic
TTAGCACCAGGTTAAAGTAA+CGG 0.608652 4.4:-42005446 None:intergenic
GGCATAGCAAGATCAAAATG+AGG 0.611060 4.4:-42003725 None:intergenic
TGACCACCAGAGTAAACACC+AGG 0.616969 4.4:-42003149 None:intergenic
GCTCATGCTACTTTCTATGG+TGG 0.618802 4.4:+42003182 MS.gene021127:CDS
CTGGCCCACTAACACCGCGT+TGG 0.620293 4.4:-42005571 None:intergenic
TGACCCCAGTTTCGGCTCAG+TGG 0.625730 4.4:-42005537 None:intergenic
GCAAGATCAAAATGAGGACG+AGG 0.630560 4.4:-42003719 None:intergenic
TGACCAAATTGCCAACTAGG+AGG 0.638092 4.4:-42005654 None:intergenic
AGGGTGCCATGCAGAAAGCG+TGG 0.647035 4.4:+42005396 MS.gene021127:intron
AACCAAGTGATAATGGTGGT+TGG 0.649043 4.4:+42003687 MS.gene021127:CDS
AGATCAAAATGAGGACGAGG+AGG 0.649689 4.4:-42003716 None:intergenic
TGAAAGGAACCCGAACCGAG+TGG 0.653524 4.4:+42005511 MS.gene021127:CDS
GATCAAAATGAGGACGAGGA+GGG 0.663864 4.4:-42003715 None:intergenic
GTCACTGCTTGAGACACGAA+AGG 0.668358 4.4:-42005601 None:intergenic
GTGCCATGCAGAAAGCGTGG+TGG 0.676430 4.4:+42005399 MS.gene021127:CDS
ATGCCACTGAGCCGAAACTG+GGG 0.678002 4.4:+42005534 MS.gene021127:CDS
AGTGTGCAAATGACAAAGAG+TGG 0.680419 4.4:+42003600 MS.gene021127:CDS
CTAACCTGCGGTAAGCAACA+GGG 0.690792 4.4:-42003787 None:intergenic
GCTCAACCAAGTGATAATGG+TGG 0.719451 4.4:+42003683 MS.gene021127:CDS
TCAGAACTGGCAATCCAACG+CGG 0.760606 4.4:+42005557 MS.gene021127:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATACTACTAAAAAAAATTA+AGG - chr4.4:42004287-42004306 None:intergenic 10.0%
!!! AAGTTTATTTTATTTTACTT+TGG + chr4.4:42004026-42004045 MS.gene021127:intron 10.0%
!!! ATTTTATCTTTAAGTTATTT+AGG + chr4.4:42003998-42004017 MS.gene021127:intron 10.0%
!!! TTAATTATCCAATTTTTATA+TGG - chr4.4:42005224-42005243 None:intergenic 10.0%
!! AAATTATCCTTATTAACTAT+TGG - chr4.4:42004898-42004917 None:intergenic 15.0%
!! AATAGTTAATAAGGATAATT+TGG + chr4.4:42004897-42004916 MS.gene021127:intron 15.0%
!! AGAAAAAATTATTATTGCAT+TGG - chr4.4:42004811-42004830 None:intergenic 15.0%
!! CTATCTATATTAAATAGAAA+TGG - chr4.4:42005185-42005204 None:intergenic 15.0%
!! GAAAAGAAAAAAAAAATTAG+AGG + chr4.4:42004209-42004228 MS.gene021127:intron 15.0%
!!! ATGTTTTTTTAGGTTTAATT+AGG - chr4.4:42003420-42003439 None:intergenic 15.0%
!!! TTATATTATTGACTTTTCTT+TGG + chr4.4:42004340-42004359 MS.gene021127:intron 15.0%
!!! TTTATGTTAATTTTTTGTGT+TGG + chr4.4:42003454-42003473 MS.gene021127:intron 15.0%
!!! TTTTCTTTTTCATTAATTGA+GGG - chr4.4:42004315-42004334 None:intergenic 15.0%
!! AAAATGGAATTAATTATGGT+AGG + chr4.4:42005277-42005296 MS.gene021127:intron 20.0%
!! AAACTAACCAATAGTTAATA+AGG + chr4.4:42004888-42004907 MS.gene021127:intron 20.0%
!! AAATGGAATTAATTATGGTA+GGG + chr4.4:42005278-42005297 MS.gene021127:intron 20.0%
!! GTAGAAAATGGAATTAATTA+TGG + chr4.4:42005273-42005292 MS.gene021127:intron 20.0%
!! TATAGTTAAGAAATTAGTTG+TGG + chr4.4:42004374-42004393 MS.gene021127:intron 20.0%
!! TTAAGGTTAAATTATATTCC+TGG - chr4.4:42004270-42004289 None:intergenic 20.0%
!!! AAATAATAGTATAAAGTACC+AGG + chr4.4:42004249-42004268 MS.gene021127:intron 20.0%
!!! ATTTTGTTGATATTTACACA+GGG + chr4.4:42005377-42005396 MS.gene021127:intron 20.0%
!!! CTTTTCTTTTTCATTAATTG+AGG - chr4.4:42004316-42004335 None:intergenic 20.0%
!!! GTAACATTTCATGTTTTTTT+AGG - chr4.4:42003430-42003449 None:intergenic 20.0%
!!! TAGTTTTAAAAGTGATACTT+TGG - chr4.4:42004874-42004893 None:intergenic 20.0%
!!! TATTTTGTTGATATTTACAC+AGG + chr4.4:42005376-42005395 MS.gene021127:intron 20.0%
! AATATTTAGGTCTCTTAACA+AGG + chr4.4:42004076-42004095 MS.gene021127:intron 25.0%
! AATCACTTTGACATATAGTT+AGG + chr4.4:42003295-42003314 MS.gene021127:intron 25.0%
! AGAAATGAATGAAGTAACTA+GGG - chr4.4:42004456-42004475 None:intergenic 25.0%
! ATCACTTTGACATATAGTTA+GGG + chr4.4:42003296-42003315 MS.gene021127:intron 25.0%
! ATGGAAAGAATGAAAAAGAA+AGG - chr4.4:42005022-42005041 None:intergenic 25.0%
! GATACAAGTGGTAAATATTT+AGG + chr4.4:42004063-42004082 MS.gene021127:intron 25.0%
! TAAAATATGAGACACTAACA+TGG - chr4.4:42005041-42005060 None:intergenic 25.0%
! TATCCACATGTTTGTATTTA+CGG - chr4.4:42003371-42003390 None:intergenic 25.0%
!! AGTGTCTCATATTTTACTTT+TGG + chr4.4:42005045-42005064 MS.gene021127:intron 25.0%
!! TTATCCAATTTTTATATGGC+AGG - chr4.4:42005220-42005239 None:intergenic 25.0%
!! TTTCACTGGAAAGAATTTTA+GGG + chr4.4:42005680-42005699 MS.gene021127:CDS 25.0%
!! TTTTCTATCAAATAACTGTC+TGG + chr4.4:42003834-42003853 MS.gene021127:intron 25.0%
!!! AAACGACACTCATTTTAAAA+CGG + chr4.4:42004980-42004999 MS.gene021127:intron 25.0%
!!! TTTTTTGTGTTGGTGTTTTT+AGG + chr4.4:42003464-42003483 MS.gene021127:intron 25.0%
AATTTGTACAGTCAAGGTTA+CGG + chr4.4:42003506-42003525 MS.gene021127:CDS 30.0%
AGAAAAAAATGCGCAAAAGA+AGG - chr4.4:42003820-42003839 None:intergenic 30.0%
AGAAAATATGAGGGCAAAAA+GGG - chr4.4:42003253-42003272 None:intergenic 30.0%
CAGAAATGAATGAAGTAACT+AGG - chr4.4:42004457-42004476 None:intergenic 30.0%
GACATAATCTCGATGTATTA+GGG - chr4.4:42003341-42003360 None:intergenic 30.0%
GTAGCAGTAATGAAAATTGA+AGG - chr4.4:42003641-42003660 None:intergenic 30.0%
GTTAAGAAATTAGTTGTGGT+AGG + chr4.4:42004378-42004397 MS.gene021127:intron 30.0%
TCAACCTGCCATATAAAAAT+TGG + chr4.4:42005213-42005232 MS.gene021127:intron 30.0%
TCAGCAATCTTAAGAAACAT+AGG - chr4.4:42003749-42003768 None:intergenic 30.0%
TCTTAAGTTTGTGATACAAG+TGG + chr4.4:42004051-42004070 MS.gene021127:intron 30.0%
TGACATAATCTCGATGTATT+AGG - chr4.4:42003342-42003361 None:intergenic 30.0%
TGTACGGAAAAAGTAGAAAA+TGG + chr4.4:42005261-42005280 MS.gene021127:intron 30.0%
! CAATTTTTGTACGAAGAACA+AGG - chr4.4:42004650-42004669 None:intergenic 30.0%
! CTTTCACTGGAAAGAATTTT+AGG + chr4.4:42005679-42005698 MS.gene021127:CDS 30.0%
!! AAGCGACATTCATTTTGAAA+CGG - chr4.4:42004745-42004764 None:intergenic 30.0%
AAATTTAGGTACGTTGACGT+AGG - chr4.4:42005114-42005133 None:intergenic 35.0%
AGACATGCATTCTTATATGC+AGG - chr4.4:42003874-42003893 None:intergenic 35.0%
ATTGTTGAAGCTAGAATTCC+TGG + chr4.4:42003131-42003150 MS.gene021127:CDS 35.0%
CAATTTGGTCAAACTTTCAC+TGG + chr4.4:42005666-42005685 MS.gene021127:CDS 35.0%
CAGAAAATATGAGGGCAAAA+AGG - chr4.4:42003254-42003273 None:intergenic 35.0%
CCTCAATCCAGAAAATATGA+GGG - chr4.4:42003262-42003281 None:intergenic 35.0%
GTGAAGTGATATAGACTGTA+CGG + chr4.4:42005245-42005264 MS.gene021127:intron 35.0%
TACGGAAATTTGTACAGTCA+AGG + chr4.4:42003500-42003519 MS.gene021127:CDS 35.0%
TCACCGTAAATACAAACATG+TGG + chr4.4:42003365-42003384 MS.gene021127:intron 35.0%
TCACTTGGTTGAGCAAAATT+AGG - chr4.4:42003677-42003696 None:intergenic 35.0%
TCCAAGATGTTGAAGATCTA+CGG - chr4.4:42005626-42005645 None:intergenic 35.0%
TGCATTCTTATATGCAGGTT+CGG - chr4.4:42003869-42003888 None:intergenic 35.0%
TTAGCACCAGGTTAAAGTAA+CGG - chr4.4:42005449-42005468 None:intergenic 35.0%
TTCAACAATGGTTTAAGCTG+TGG + chr4.4:42003560-42003579 MS.gene021127:CDS 35.0%
TTCATTAACTGTGATGAGAC+TGG - chr4.4:42004407-42004426 None:intergenic 35.0%
TTTGCTCAACCAAGTGATAA+TGG + chr4.4:42003680-42003699 MS.gene021127:CDS 35.0%
! AGATGGTGTTTTCGACTATT+TGG + chr4.4:42004502-42004521 MS.gene021127:intron 35.0%
! CCTCATATTTTCTGGATTGA+GGG + chr4.4:42003260-42003279 MS.gene021127:intron 35.0%
! CTTTTTGCCCTCATATTTTC+TGG + chr4.4:42003252-42003271 MS.gene021127:intron 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG - chr4.4:42004849-42004868 None:intergenic 35.0%
! TGTGCAGAGTATTAATTGGA+GGG - chr4.4:42004845-42004864 None:intergenic 35.0%
!! GAAAGCAATGTAGAGCATTT+TGG - chr4.4:42003090-42003109 None:intergenic 35.0%
!! GGTAATTTGTTACATACCCA+TGG - chr4.4:42003225-42003244 None:intergenic 35.0%
!!! GACACTCATTTTAAAACGGA+GGG + chr4.4:42004984-42005003 MS.gene021127:intron 35.0%
!!! TTTGTGTTGGTGTTTTTAGG+AGG + chr4.4:42003467-42003486 MS.gene021127:intron 35.0%
ACCGTAGATCTTCAACATCT+TGG + chr4.4:42005622-42005641 MS.gene021127:CDS 40.0%
AGAATTCCTGGTGTTTACTC+TGG + chr4.4:42003143-42003162 MS.gene021127:CDS 40.0%
AGTGTGCAAATGACAAAGAG+TGG + chr4.4:42003600-42003619 MS.gene021127:CDS 40.0%
ATGAGGGCAAAAAGGGAAAT+GGG - chr4.4:42003246-42003265 None:intergenic 40.0%
CCCTCAATCCAGAAAATATG+AGG - chr4.4:42003263-42003282 None:intergenic 40.0%
CTTGGTTGAGCAAAATTAGG+AGG - chr4.4:42003674-42003693 None:intergenic 40.0%
GAAGGAAAGTAACTAACCTG+CGG - chr4.4:42003802-42003821 None:intergenic 40.0%
GCATTCTTATATGCAGGTTC+GGG - chr4.4:42003868-42003887 None:intergenic 40.0%
GGAATAAGATTCACCGTGAA+CGG + chr4.4:42005420-42005439 MS.gene021127:CDS 40.0%
GGCATAGCAAGATCAAAATG+AGG - chr4.4:42003728-42003747 None:intergenic 40.0%
GTGTCTCTGATTCATGAAGT+GGG + chr4.4:42004557-42004576 MS.gene021127:intron 40.0%
GTTTGACCAAATTGCCAACT+AGG - chr4.4:42005660-42005679 None:intergenic 40.0%
TATGAGGGCAAAAAGGGAAA+TGG - chr4.4:42003247-42003266 None:intergenic 40.0%
TGCTCTAGTCAAATGTGAGA+TGG + chr4.4:42004485-42004504 MS.gene021127:intron 40.0%
TGTCTCTGATTCATGAAGTG+GGG + chr4.4:42004558-42004577 MS.gene021127:intron 40.0%
TGTGTCTCTGATTCATGAAG+TGG + chr4.4:42004556-42004575 MS.gene021127:intron 40.0%
! AAAGTAACGGAATCCGTTCA+CGG - chr4.4:42005436-42005455 None:intergenic 40.0%
! AGCACTGCTTTGTTCAACAA+TGG + chr4.4:42003548-42003567 MS.gene021127:CDS 40.0%
! CCCTCATATTTTCTGGATTG+AGG + chr4.4:42003259-42003278 MS.gene021127:intron 40.0%
! GTGTGCAGAGTATTAATTGG+AGG - chr4.4:42004846-42004865 None:intergenic 40.0%
!! AACCAAGTGATAATGGTGGT+TGG + chr4.4:42003687-42003706 MS.gene021127:CDS 40.0%
!! GATGCTCATGCTACTTTCTA+TGG + chr4.4:42003179-42003198 MS.gene021127:CDS 40.0%
!!! CGACACTCATTTTAAAACGG+AGG + chr4.4:42004983-42005002 MS.gene021127:intron 40.0%
!!! TGTTTTTAGGAGGAGCATGT+GGG + chr4.4:42003477-42003496 MS.gene021127:intron 40.0%
AGATCAAAATGAGGACGAGG+AGG - chr4.4:42003719-42003738 None:intergenic 45.0%
ATTCCTGGTGTTTACTCTGG+TGG + chr4.4:42003146-42003165 MS.gene021127:CDS 45.0%
CACCAACCACCATTATCACT+TGG - chr4.4:42003692-42003711 None:intergenic 45.0%
CTACGTTGCTAATTAGCACC+AGG - chr4.4:42005461-42005480 None:intergenic 45.0%
GATCAAAATGAGGACGAGGA+GGG - chr4.4:42003718-42003737 None:intergenic 45.0%
GCAAGATCAAAATGAGGACG+AGG - chr4.4:42003722-42003741 None:intergenic 45.0%
GTGCTAATTAGCAACGTAGC+AGG + chr4.4:42005462-42005481 MS.gene021127:CDS 45.0%
GTGTTTACTCTGGTGGTCAA+TGG + chr4.4:42003153-42003172 MS.gene021127:CDS 45.0%
TGACCAAATTGCCAACTAGG+AGG - chr4.4:42005657-42005676 None:intergenic 45.0%
! AAGATTGCTGAGTATCGTGC+TGG + chr4.4:42003758-42003777 MS.gene021127:CDS 45.0%
! CGGATTCCGTTACTTTAACC+TGG + chr4.4:42005440-42005459 MS.gene021127:CDS 45.0%
!! AAGTGGGGTAGTGTTACTTC+GGG + chr4.4:42004573-42004592 MS.gene021127:intron 45.0%
!! GAAGTGGGGTAGTGTTACTT+CGG + chr4.4:42004572-42004591 MS.gene021127:intron 45.0%
!! GACAAAGAGTGGTGTCATTC+TGG + chr4.4:42003611-42003630 MS.gene021127:CDS 45.0%
!! GCTCAACCAAGTGATAATGG+TGG + chr4.4:42003683-42003702 MS.gene021127:CDS 45.0%
!! GCTCATGCTACTTTCTATGG+TGG + chr4.4:42003182-42003201 MS.gene021127:CDS 45.0%
!! GGTAGTGTTACTTCGGGTAA+AGG + chr4.4:42004579-42004598 MS.gene021127:intron 45.0%
!! TATGGTGGATCTGATGCTTC+TGG + chr4.4:42003197-42003216 MS.gene021127:CDS 45.0%
!!! GTGTTTTTAGGAGGAGCATG+TGG + chr4.4:42003476-42003495 MS.gene021127:intron 45.0%
!! AAAAAAAAAAATGAAAATTT+AGG - chr4.4:42005128-42005147 None:intergenic 5.0%
!!! ATTATTTTTAGATTTTATTT+TGG - chr4.4:42004236-42004255 None:intergenic 5.0%
!!! TTCATTTTTTTTTTATTTTT+TGG + chr4.4:42003935-42003954 MS.gene021127:intron 5.0%
ACTAACCTGCGGTAAGCAAC+AGG - chr4.4:42003791-42003810 None:intergenic 50.0%
AGACACGAAAGGACAAAGCC+TGG - chr4.4:42005593-42005612 None:intergenic 50.0%
ATCTGATGCTTCTGGCACCA+TGG + chr4.4:42003205-42003224 MS.gene021127:CDS 50.0%
ATTAGCAACGTAGCAGGTGC+TGG + chr4.4:42005468-42005487 MS.gene021127:CDS 50.0%
ATTCCACCACGCTTTCTGCA+TGG - chr4.4:42005405-42005424 None:intergenic 50.0%
CTAACCTGCGGTAAGCAACA+GGG - chr4.4:42003790-42003809 None:intergenic 50.0%
GCTCCTCCTAGTTGGCAATT+TGG + chr4.4:42005651-42005670 MS.gene021127:CDS 50.0%
GGAACATTGCTCCTCCTAGT+TGG + chr4.4:42005643-42005662 MS.gene021127:CDS 50.0%
GTCACTGCTTGAGACACGAA+AGG - chr4.4:42005604-42005623 None:intergenic 50.0%
TCAGAACTGGCAATCCAACG+CGG + chr4.4:42005557-42005576 MS.gene021127:CDS 50.0%
TCCAATGAGCTTTGCTCACG+AGG + chr4.4:42003896-42003915 MS.gene021127:intron 50.0%
TCCTCGTGAGCAAAGCTCAT+TGG - chr4.4:42003900-42003919 None:intergenic 50.0%
TCTGATGCTTCTGGCACCAT+GGG + chr4.4:42003206-42003225 MS.gene021127:CDS 50.0%
TGACCACCAGAGTAAACACC+AGG - chr4.4:42003152-42003171 None:intergenic 50.0%
! CAATCCAACGCGGTGTTAGT+GGG + chr4.4:42005567-42005586 MS.gene021127:CDS 50.0%
! TTAGGAGGAGCATGTGGGTA+CGG + chr4.4:42003482-42003501 MS.gene021127:intron 50.0%
ATGCCACTGAGCCGAAACTG+GGG + chr4.4:42005534-42005553 MS.gene021127:CDS 55.0%
GATGCCACTGAGCCGAAACT+GGG + chr4.4:42005533-42005552 MS.gene021127:CDS 55.0%
TCAGTGGCATCCACTCGGTT+CGG - chr4.4:42005524-42005543 None:intergenic 55.0%
TGAAAGGAACCCGAACCGAG+TGG + chr4.4:42005511-42005530 MS.gene021127:CDS 55.0%
! GCAATCCAACGCGGTGTTAG+TGG + chr4.4:42005566-42005585 MS.gene021127:CDS 55.0%
! GCCAGTTCTGACCCCAGTTT+CGG - chr4.4:42005548-42005567 None:intergenic 55.0%
! GGTTCGGGTTCCTTTCACGT+AGG - chr4.4:42005508-42005527 None:intergenic 55.0%
! TTGTCCCTGTTGCTTACCGC+AGG + chr4.4:42003783-42003802 MS.gene021127:CDS 55.0%
ATCGTGCGCGCCTACGTGAA+AGG + chr4.4:42005495-42005514 MS.gene021127:CDS 60.0%
CAGTGGCATCCACTCGGTTC+GGG - chr4.4:42005523-42005542 None:intergenic 60.0%
GCCGAAACTGGGGTCAGAAC+TGG + chr4.4:42005544-42005563 MS.gene021127:CDS 60.0%
GGATGCCACTGAGCCGAAAC+TGG + chr4.4:42005532-42005551 MS.gene021127:CDS 60.0%
TCGGCTCAGTGGCATCCACT+CGG - chr4.4:42005529-42005548 None:intergenic 60.0%
TGACCCCAGTTTCGGCTCAG+TGG - chr4.4:42005540-42005559 None:intergenic 60.0%
! AGGGTGCCATGCAGAAAGCG+TGG + chr4.4:42005396-42005415 MS.gene021127:intron 60.0%
! GTGCCATGCAGAAAGCGTGG+TGG + chr4.4:42005399-42005418 MS.gene021127:CDS 60.0%
CTGGCCCACTAACACCGCGT+TGG - chr4.4:42005574-42005593 None:intergenic 65.0%
! CAACGCGGTGTTAGTGGGCC+AGG + chr4.4:42005572-42005591 MS.gene021127:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 42003080 42005707 42003080 ID=MS.gene021127
chr4.4 mRNA 42003080 42005707 42003080 ID=MS.gene021127.t1;Parent=MS.gene021127
chr4.4 exon 42003080 42003227 42003080 ID=MS.gene021127.t1.exon1;Parent=MS.gene021127.t1
chr4.4 CDS 42003080 42003227 42003080 ID=cds.MS.gene021127.t1;Parent=MS.gene021127.t1
chr4.4 exon 42003486 42003804 42003486 ID=MS.gene021127.t1.exon2;Parent=MS.gene021127.t1
chr4.4 CDS 42003486 42003804 42003486 ID=cds.MS.gene021127.t1;Parent=MS.gene021127.t1
chr4.4 exon 42005398 42005707 42005398 ID=MS.gene021127.t1.exon3;Parent=MS.gene021127.t1
chr4.4 CDS 42005398 42005707 42005398 ID=cds.MS.gene021127.t1;Parent=MS.gene021127.t1
Gene Sequence

>MS.gene021127

ATGGTGGCCAAAATGCTCTACATTGCTTTCATAATTATCATTTCAATGTACATTGTTGAAGCTAGAATTCCTGGTGTTTACTCTGGTGGTCAATGGCAAGATGCTCATGCTACTTTCTATGGTGGATCTGATGCTTCTGGCACCATGGGTATGTAACAAATTACCCATTTCCCTTTTTGCCCTCATATTTTCTGGATTGAGGGTGAGCTTGACAAAATCACTTTGACATATAGTTAGGGTTACATGTATAGCTTAAAACCCTAATACATCGAGATTATGTCAAATTCACCGTAAATACAAACATGTGGATAAAATACATACAAATAATTACAATGTACCTAATTAAACCTAAAAAAACATGAAATGTTACTATGTTTATGTTAATTTTTTGTGTTGGTGTTTTTAGGAGGAGCATGTGGGTACGGAAATTTGTACAGTCAAGGTTACGGAGTGAACACAGCAGCATTAAGCACTGCTTTGTTCAACAATGGTTTAAGCTGTGGTGCTTGTTTTGAGATAAAGTGTGCAAATGACAAAGAGTGGTGTCATTCTGGAAGTCCTTCAATTTTCATTACTGCTACTAACTTTTGTCCTCCTAATTTTGCTCAACCAAGTGATAATGGTGGTTGGTGTAACCCTCCTCGTCCTCATTTTGATCTTGCTATGCCTATGTTTCTTAAGATTGCTGAGTATCGTGCTGGTATTGTCCCTGTTGCTTACCGCAGGTTAGTTACTTTCCTTCTTTTGCGCATTTTTTTCTATCAAATAACTGTCTGGTGTTCGAACCCGAACCTGCATATAAGAATGCATGTCTCTTCCAATGAGCTTTGCTCACGAGGACTGCTCTTTTACTACTTCATTTTTTTTTTATTTTTTGGTATAAACTTCATTTTTAATTTTAGATTAATTAAGTTATACATTTTATCTTTAAGTTATTTAGGTCTCTAAGTTTATTTTATTTTACTTTGGTGTCTTAAGTTTGTGATACAAGTGGTAAATATTTAGGTCTCTTAACAAGGAAAAACATTTTGTTTCAATTTTTAAATAAAGTCTATAATGCTGTTAGAACAAAAAACAATTTATAGAGACAAAGTGTATAGTACTTGTCTGTCACTTAAATAACTAAAATGAAAAGAAAAAAAAAATTAGAGGACCAAAATAAAATCTAAAAATAATAGTATAAAGTACCAGGAATATAATTTAACCTTAATTTTTTTTAGTAGTATAGTACTCCCTCAATTAATGAAAAAGAAAAGTAGTTTATATTATTGACTTTTCTTTGGAAAATGATGTTTATAGTTAAGAAATTAGTTGTGGTAGGAGTCCAGTCTCATCACAGTTAATGAAAAAAGAAAAGTAGCTTATATTATTGACCCTAGTTACTTCATTCATTTCTGTATATAGTTGCTCTAGTCAAATGTGAGATGGTGTTTTCGACTATTTGGCTATGTAAATTTCTGCACTTCTCACATGATCTGTGTCTCTGATTCATGAAGTGGGGTAGTGTTACTTCGGGTAAAGGATTCTCTTACACACGTATCTTTTTTCTACTGATCACTCACTCTTTCCTTGTTCTTCGTACAAAAATTGTTCGTACAAATATAGCATTGAGCTGTTTAGACGAAATTAAGTTATACGTATACTTGAAAAAAATTACTCTCTCCGTTTCAAAATGAATGTCGCTTTAGCTAAAAAAATTTGTTTCAAAATGAGTGTTGCTTTCAGTTTCCAATGCAATAATAATTTTTTCTTTTCAATTGTACCCTCCAATTAATACTCTGCACACTACTTCCAAAGTATCACTTTTAAAACTAACCAATAGTTAATAAGGATAATTTGGTAAAATAATCATTCTCTTTCTCTTAATCATTGCATTTCTTAATATGTGTGTAAACATCTAAAACGACACTCATTTTAAAACGGAGGGAGTATATATGTTCCTTTCTTTTTCATTCTTTCCATGTTAGTGTCTCATATTTTACTTTTGGAATCATTTTTCTTTTACTTTTTTAGTCACTTTCAGTGAAATTACCTACGTCAACGTACCTAAATTTTCATTTTTTTTTTTATAATTTATTTATTTTTCTTAACTATATATATGTCCATTTCTATTTAATATAGATAGATATCTACTCAACCTGCCATATAAAAATTGGATAATTAAAGTGAAGTGATATAGACTGTACGGAAAAAGTAGAAAATGGAATTAATTATGGTAGGGTATAGCTAGCATGTGTAAAAATAAAAAAGATAAGTGAAAAAGTTAATAAAATGTGAAAAGTCAAAAACAGTGACGTATTTTGTTGATATTTACACAGGGTGCCATGCAGAAAGCGTGGTGGAATAAGATTCACCGTGAACGGATTCCGTTACTTTAACCTGGTGCTAATTAGCAACGTAGCAGGTGCTGGTGATATCGTGCGCGCCTACGTGAAAGGAACCCGAACCGAGTGGATGCCACTGAGCCGAAACTGGGGTCAGAACTGGCAATCCAACGCGGTGTTAGTGGGCCAGGCTTTGTCCTTTCGTGTCTCAAGCAGTGACCGTAGATCTTCAACATCTTGGAACATTGCTCCTCCTAGTTGGCAATTTGGTCAAACTTTCACTGGAAAGAATTTTAGGGTTTAA
Protein sequence

>MS.gene021127.t1

MVAKMLYIAFIIIISMYIVEARIPGVYSGGQWQDAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNNGLSCGACFEIKCANDKEWCHSGSPSIFITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKIAEYRAGIVPVAYRRVPCRKRGGIRFTVNGFRYFNLVLISNVAGAGDIVRAYVKGTRTEWMPLSRNWGQNWQSNAVLVGQALSFRVSSSDRRSSTSWNIAPPSWQFGQTFTGKNFRV