Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022341.t1 | XP_003613127.2 | 100 | 147 | 0 | 0 | 1 | 147 | 1 | 147 | 9.30E-52 | 213 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022341.t1 | P49637 | 77.6 | 147 | 32 | 1 | 1 | 147 | 1 | 146 | 6.1e-44 | 178.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022341.t1 | G7JYX5 | 100.0 | 147 | 0 | 0 | 1 | 147 | 1 | 147 | 6.7e-52 | 213.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene022341 | MS.gene44297 | PPI |
| MS.gene022341 | MS.gene74837 | PPI |
| MS.gene022341 | MS.gene26005 | PPI |
| MS.gene26005 | MS.gene022341 | PPI |
| MS.gene70668 | MS.gene022341 | PPI |
| MS.gene022341 | MS.gene86214 | PPI |
| MS.gene022341 | MS.gene027718 | PPI |
| MS.gene022341 | MS.gene29174 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022341.t1 | MTR_5g033090 | 100.000 | 147 | 0 | 0 | 1 | 147 | 1 | 147 | 2.98e-104 | 294 |
| MS.gene022341.t1 | MTR_3g077050 | 99.320 | 147 | 1 | 0 | 1 | 147 | 1 | 147 | 7.09e-104 | 293 |
| MS.gene022341.t1 | MTR_8g069460 | 84.459 | 148 | 22 | 1 | 1 | 147 | 1 | 148 | 8.14e-88 | 253 |
| MS.gene022341.t1 | MTR_4g119380 | 53.741 | 147 | 14 | 3 | 1 | 147 | 100 | 192 | 3.35e-36 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022341.t1 | AT1G70600 | 85.714 | 147 | 20 | 1 | 1 | 147 | 1 | 146 | 9.47e-89 | 255 |
| MS.gene022341.t1 | AT1G23290 | 83.673 | 147 | 23 | 1 | 1 | 147 | 1 | 146 | 3.32e-86 | 248 |
| MS.gene022341.t1 | AT1G12960 | 50.000 | 146 | 27 | 2 | 1 | 146 | 1 | 100 | 1.75e-36 | 121 |
Find 40 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGGGTTCTTTAAGCTTCT+TGG | 0.217196 | 3.4:+74063341 | MS.gene022341:CDS |
| GTGATTGATGTTACTCAGTT+TGG | 0.327888 | 3.4:+74063323 | MS.gene022341:CDS |
| TGATTGATGTTACTCAGTTT+GGG | 0.380454 | 3.4:+74063324 | MS.gene022341:CDS |
| TTCTTTAAGCTTCTTGGAAA+AGG | 0.391337 | 3.4:+74063347 | MS.gene022341:CDS |
| TGTGAAGGAGAAAGCTTTGA+AGG | 0.400500 | 3.4:+74063286 | MS.gene022341:CDS |
| ATCTTGTCGATGTTGACAAT+AGG | 0.419901 | 3.4:-74063242 | None:intergenic |
| TCTTGTCGATGTTGACAATA+GGG | 0.425312 | 3.4:-74063241 | None:intergenic |
| GCTTTCTCCTTCACATCTTG+TGG | 0.427470 | 3.4:-74063278 | None:intergenic |
| AGCTTAGTCTTGACAACAAA+AGG | 0.442858 | 3.4:-74063389 | None:intergenic |
| TCCGGCGTTACCACGACCTC+CGG | 0.443108 | 3.4:-74063117 | None:intergenic |
| CGCAAGCATCCCGGAGGTCG+TGG | 0.447863 | 3.4:+74063107 | MS.gene022341:CDS |
| CTTCCTGTTCTTCTTGAAGC+GGG | 0.447997 | 3.4:-74063039 | None:intergenic |
| CGACCCGCTTCAAGAAGAAC+AGG | 0.481196 | 3.4:+74063036 | MS.gene022341:CDS |
| ACGATGGTGGTGCATTCCTC+CGG | 0.496860 | 3.4:-74063135 | None:intergenic |
| CCAACCTTACCGAAGTAACC+TGG | 0.506573 | 3.4:-74063179 | None:intergenic |
| CTTTCCGATACGACCGTGAC+CGG | 0.524646 | 3.4:-74063081 | None:intergenic |
| CCAGGTTACTTCGGTAAGGT+TGG | 0.536880 | 3.4:+74063179 | MS.gene022341:CDS |
| TCTTCCTGTTCTTCTTGAAG+CGG | 0.538920 | 3.4:-74063040 | None:intergenic |
| TAGTCTTGACAACAAAAGGC+TGG | 0.539190 | 3.4:-74063385 | None:intergenic |
| AGCGCCGGTCACGGTCGTAT+CGG | 0.544455 | 3.4:+74063077 | MS.gene022341:CDS |
| GATTGCTGAAAAGAAGATCA+AGG | 0.548421 | 3.4:+74063421 | MS.gene022341:CDS |
| TCATCCAGGTTACTTCGGTA+AGG | 0.549171 | 3.4:+74063175 | MS.gene022341:CDS |
| CTCTTCGACAAGTATCATCC+AGG | 0.553418 | 3.4:+74063161 | MS.gene022341:CDS |
| ACAACAAAAGGCTGGTTCTG+AGG | 0.563935 | 3.4:-74063377 | None:intergenic |
| TCTCATTCCACAAGATGTGA+AGG | 0.564862 | 3.4:+74063271 | MS.gene022341:CDS |
| GAAAAGAAGATCAAGGAAGC+TGG | 0.567856 | 3.4:+74063428 | MS.gene022341:CDS |
| GGTCACGTGAGCGCCGGTCA+CGG | 0.569135 | 3.4:+74063068 | MS.gene022341:CDS |
| CCCGGAGGTCGTGGTAACGC+CGG | 0.572163 | 3.4:+74063116 | MS.gene022341:CDS |
| AAGAGAGGTCACGTGAGCGC+CGG | 0.573118 | 3.4:+74063062 | MS.gene022341:CDS |
| CCGGCGTTACCACGACCTCC+GGG | 0.573655 | 3.4:-74063116 | None:intergenic |
| AAGTATCATCCAGGTTACTT+CGG | 0.578842 | 3.4:+74063170 | MS.gene022341:CDS |
| GGAAAGCATCGCAAGCATCC+CGG | 0.586004 | 3.4:+74063098 | MS.gene022341:CDS |
| AAGAAGAACAGGAAGAAGAG+AGG | 0.594785 | 3.4:+74063047 | MS.gene022341:CDS |
| CTGGATGATACTTGTCGAAG+AGG | 0.597192 | 3.4:-74063160 | None:intergenic |
| AACTGAGTAACATCAATCAC+AGG | 0.611236 | 3.4:-74063320 | None:intergenic |
| GGAGGTCGTGGTAACGCCGG+AGG | 0.620157 | 3.4:+74063119 | MS.gene022341:CDS |
| AAGAAGATCAAGGAAGCTGG+TGG | 0.687960 | 3.4:+74063431 | MS.gene022341:CDS |
| ACTTGTCGAAGAGGATACGA+TGG | 0.691930 | 3.4:-74063151 | None:intergenic |
| TGTCGAAGAGGATACGATGG+TGG | 0.711648 | 3.4:-74063148 | None:intergenic |
| AAGCATCGCAAGCATCCCGG+AGG | 0.718770 | 3.4:+74063101 | MS.gene022341:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGATTGATGTTACTCAGTTT+GGG | + | chr3.4:74063324-74063343 | MS.gene022341:CDS | 30.0% | |
| TTCTTTAAGCTTCTTGGAAA+AGG | + | chr3.4:74063347-74063366 | MS.gene022341:CDS | 30.0% | |
| AACTGAGTAACATCAATCAC+AGG | - | chr3.4:74063323-74063342 | None:intergenic | 35.0% | |
| AAGTATCATCCAGGTTACTT+CGG | + | chr3.4:74063170-74063189 | MS.gene022341:CDS | 35.0% | |
| ATCTTGTCGATGTTGACAAT+AGG | - | chr3.4:74063245-74063264 | None:intergenic | 35.0% | |
| GATTGCTGAAAAGAAGATCA+AGG | + | chr3.4:74063421-74063440 | MS.gene022341:CDS | 35.0% | |
| GTGATTGATGTTACTCAGTT+TGG | + | chr3.4:74063323-74063342 | MS.gene022341:CDS | 35.0% | |
| TCTTGTCGATGTTGACAATA+GGG | - | chr3.4:74063244-74063263 | None:intergenic | 35.0% | |
| ! | TTTGGGTTCTTTAAGCTTCT+TGG | + | chr3.4:74063341-74063360 | MS.gene022341:CDS | 35.0% |
| !! | AGCTTAGTCTTGACAACAAA+AGG | - | chr3.4:74063392-74063411 | None:intergenic | 35.0% |
| AAGAAGAACAGGAAGAAGAG+AGG | + | chr3.4:74063047-74063066 | MS.gene022341:CDS | 40.0% | |
| GAAAAGAAGATCAAGGAAGC+TGG | + | chr3.4:74063428-74063447 | MS.gene022341:CDS | 40.0% | |
| TCTCATTCCACAAGATGTGA+AGG | + | chr3.4:74063271-74063290 | MS.gene022341:CDS | 40.0% | |
| TCTTCCTGTTCTTCTTGAAG+CGG | - | chr3.4:74063043-74063062 | None:intergenic | 40.0% | |
| ! | TAGTCTTGACAACAAAAGGC+TGG | - | chr3.4:74063388-74063407 | None:intergenic | 40.0% |
| ! | TGTGAAGGAGAAAGCTTTGA+AGG | + | chr3.4:74063286-74063305 | MS.gene022341:CDS | 40.0% |
| AAGAAGATCAAGGAAGCTGG+TGG | + | chr3.4:74063431-74063450 | MS.gene022341:CDS | 45.0% | |
| ACTTGTCGAAGAGGATACGA+TGG | - | chr3.4:74063154-74063173 | None:intergenic | 45.0% | |
| CTCTTCGACAAGTATCATCC+AGG | + | chr3.4:74063161-74063180 | MS.gene022341:CDS | 45.0% | |
| CTGGATGATACTTGTCGAAG+AGG | - | chr3.4:74063163-74063182 | None:intergenic | 45.0% | |
| CTTCCTGTTCTTCTTGAAGC+GGG | - | chr3.4:74063042-74063061 | None:intergenic | 45.0% | |
| GCTTTCTCCTTCACATCTTG+TGG | - | chr3.4:74063281-74063300 | None:intergenic | 45.0% | |
| TCATCCAGGTTACTTCGGTA+AGG | + | chr3.4:74063175-74063194 | MS.gene022341:CDS | 45.0% | |
| !! | ACAACAAAAGGCTGGTTCTG+AGG | - | chr3.4:74063380-74063399 | None:intergenic | 45.0% |
| CCAACCTTACCGAAGTAACC+TGG | - | chr3.4:74063182-74063201 | None:intergenic | 50.0% | |
| CCAGGTTACTTCGGTAAGGT+TGG | + | chr3.4:74063179-74063198 | MS.gene022341:CDS | 50.0% | |
| TGTCGAAGAGGATACGATGG+TGG | - | chr3.4:74063151-74063170 | None:intergenic | 50.0% | |
| CGACCCGCTTCAAGAAGAAC+AGG | + | chr3.4:74063036-74063055 | MS.gene022341:CDS | 55.0% | |
| CTTTCCGATACGACCGTGAC+CGG | - | chr3.4:74063084-74063103 | None:intergenic | 55.0% | |
| ! | ACGATGGTGGTGCATTCCTC+CGG | - | chr3.4:74063138-74063157 | None:intergenic | 55.0% |
| !! | GGAAAGCATCGCAAGCATCC+CGG | + | chr3.4:74063098-74063117 | MS.gene022341:CDS | 55.0% |
| AAGAGAGGTCACGTGAGCGC+CGG | + | chr3.4:74063062-74063081 | MS.gene022341:CDS | 60.0% | |
| ! | AAGCATCGCAAGCATCCCGG+AGG | + | chr3.4:74063101-74063120 | MS.gene022341:CDS | 60.0% |
| TCCGGCGTTACCACGACCTC+CGG | - | chr3.4:74063120-74063139 | None:intergenic | 65.0% | |
| !! | AGCGCCGGTCACGGTCGTAT+CGG | + | chr3.4:74063077-74063096 | MS.gene022341:CDS | 65.0% |
| CCGGCGTTACCACGACCTCC+GGG | - | chr3.4:74063119-74063138 | None:intergenic | 70.0% | |
| CGCAAGCATCCCGGAGGTCG+TGG | + | chr3.4:74063107-74063126 | MS.gene022341:CDS | 70.0% | |
| ! | CCCGGAGGTCGTGGTAACGC+CGG | + | chr3.4:74063116-74063135 | MS.gene022341:CDS | 70.0% |
| ! | GGAGGTCGTGGTAACGCCGG+AGG | + | chr3.4:74063119-74063138 | MS.gene022341:CDS | 70.0% |
| !! | GGTCACGTGAGCGCCGGTCA+CGG | + | chr3.4:74063068-74063087 | MS.gene022341:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 74063032 | 74063475 | 74063032 | ID=MS.gene022341 |
| chr3.4 | mRNA | 74063032 | 74063475 | 74063032 | ID=MS.gene022341.t1;Parent=MS.gene022341 |
| chr3.4 | exon | 74063032 | 74063475 | 74063032 | ID=MS.gene022341.t1.exon1;Parent=MS.gene022341.t1 |
| chr3.4 | CDS | 74063032 | 74063475 | 74063032 | ID=cds.MS.gene022341.t1;Parent=MS.gene022341.t1 |
| Gene Sequence |
| Protein sequence |
