AlfalfaGEDB | MS.gene70668

M. sativa cultivar XinJiangDaYe / MS.gene70668

KEGG: K02900
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00828.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70668.t1	RHN68732.1	95.6	160	6	1	1	160	32	190	1.40E-53	219.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70668.t1	P49637	77.6	147	32	1	14	160	1	146	6.7e-44	178.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70668.t1	A0A396IT43	95.6	160	6	1	1	160	32	190	1.0e-53	219.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene70668	MS.gene86214	PPI
MS.gene70668	MS.gene027718	PPI
MS.gene70668	MS.gene29174	PPI
MS.gene70668	MS.gene44297	PPI
MS.gene70668	MS.gene74837	PPI
MS.gene70668	MS.gene26005	PPI
MS.gene70668	MS.gene022341	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70668.t1	MTR_5g033090	100.000	147	0	0	14	160	1	147	3.65e-104	295
MS.gene70668.t1	MTR_3g077050	99.320	147	1	0	14	160	1	147	1.18e-103	293
MS.gene70668.t1	MTR_8g069460	84.459	148	22	1	14	160	1	148	9.57e-88	253
MS.gene70668.t1	MTR_4g119380	55.263	152	14	3	9	160	95	192	1.09e-39	133

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70668.t1	AT1G70600	85.714	147	20	1	14	160	1	146	1.28e-88	255
MS.gene70668.t1	AT1G23290	83.673	147	23	1	14	160	1	146	3.45e-86	249
MS.gene70668.t1	AT1G12960	50.000	146	27	2	14	159	1	100	2.48e-36	121

Find 41 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGGGTTCTTTAAGCTTCT+TGG	0.217196	3.1:+63435998	MS.gene70668:CDS
GTGATTGATGTTACTCAGTT+TGG	0.327888	3.1:+63435980	MS.gene70668:CDS
TGATTGATGTTACTCAGTTT+GGG	0.380454	3.1:+63435981	MS.gene70668:CDS
TTCTTTAAGCTTCTTGGAAA+AGG	0.391337	3.1:+63436004	MS.gene70668:CDS
TGTGAAGGAGAAAGCTTTGA+AGG	0.400500	3.1:+63435943	MS.gene70668:CDS
ATCTTGTCGATGTTGACAAT+AGG	0.419901	3.1:-63435899	None:intergenic
TCTTGTCGATGTTGACAATA+GGG	0.425312	3.1:-63435898	None:intergenic
AGCTTAGTCTTGACAACAAA+AGG	0.442858	3.1:-63436046	None:intergenic
TCCGGCGTTACCACGACCTC+CGG	0.443108	3.1:-63435774	None:intergenic
CGCAAGCATCCCGGAGGTCG+TGG	0.447863	3.1:+63435764	MS.gene70668:CDS
CTTCCTGTTCTTCTTGAAGC+GGG	0.447997	3.1:-63435696	None:intergenic
GCTTTCTCCTTCACATCTTG+AGG	0.470251	3.1:-63435935	None:intergenic
CGACCCGCTTCAAGAAGAAC+AGG	0.481196	3.1:+63435693	MS.gene70668:CDS
ACGGTGATGGTGCATTCCTC+CGG	0.496860	3.1:-63435792	None:intergenic
CCAACCTTACCGAAGTAACC+TGG	0.506573	3.1:-63435836	None:intergenic
GATACTTGTCGAAGAGGATA+CGG	0.513304	3.1:-63435811	None:intergenic
CCAGGTTACTTCGGTAAGGT+TGG	0.536880	3.1:+63435836	MS.gene70668:CDS
TCTTCCTGTTCTTCTTGAAG+CGG	0.538920	3.1:-63435697	None:intergenic
GGTCACGTGAGCGCCGGTCA+TGG	0.538972	3.1:+63435725	MS.gene70668:CDS
TAGTCTTGACAACAAAAGGC+TGG	0.539190	3.1:-63436042	None:intergenic
GATTGCTGAAAAGAAGATCA+AGG	0.548421	3.1:+63436078	MS.gene70668:CDS
TCATCCAGGTTACTTCGGTA+AGG	0.549171	3.1:+63435832	MS.gene70668:CDS
CTCTTCGACAAGTATCATCC+AGG	0.553418	3.1:+63435818	MS.gene70668:CDS
CTTTCCGATACGACCATGAC+CGG	0.562794	3.1:-63435738	None:intergenic
ACAACAAAAGGCTGGTTCTG+AGG	0.563935	3.1:-63436034	None:intergenic
TCTCATTCCTCAAGATGTGA+AGG	0.564862	3.1:+63435928	MS.gene70668:CDS
GAAAAGAAGATCAAGGAAGC+TGG	0.567856	3.1:+63436085	MS.gene70668:CDS
CCCGGAGGTCGTGGTAACGC+CGG	0.572163	3.1:+63435773	MS.gene70668:CDS
AAGAGAGGTCACGTGAGCGC+CGG	0.573118	3.1:+63435719	MS.gene70668:CDS
CCGGCGTTACCACGACCTCC+GGG	0.573655	3.1:-63435773	None:intergenic
AGCGCCGGTCATGGTCGTAT+CGG	0.577785	3.1:+63435734	MS.gene70668:CDS
AAGTATCATCCAGGTTACTT+CGG	0.578842	3.1:+63435827	MS.gene70668:CDS
GGAAAGCATCGCAAGCATCC+CGG	0.586004	3.1:+63435755	MS.gene70668:CDS
AAGAAGAACAGGAAGAAGAG+AGG	0.594785	3.1:+63435704	MS.gene70668:CDS
CTGGATGATACTTGTCGAAG+AGG	0.597192	3.1:-63435817	None:intergenic
AGCGGGTCGTCATCTTCGTG+AGG	0.605705	3.1:-63435679	None:intergenic
AACTGAGTAACATCAATCAC+AGG	0.611236	3.1:-63435977	None:intergenic
GGAGGTCGTGGTAACGCCGG+AGG	0.620157	3.1:+63435776	MS.gene70668:CDS
TGTCGAAGAGGATACGGTGA+TGG	0.642158	3.1:-63435805	None:intergenic
AAGAAGATCAAGGAAGCTGG+TGG	0.687960	3.1:+63436088	MS.gene70668:CDS
AAGCATCGCAAGCATCCCGG+AGG	0.718770	3.1:+63435758	MS.gene70668:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TGATTGATGTTACTCAGTTT+GGG	+	chr3.1:63435981-63436000	MS.gene70668:CDS	30.0%
	TTCTTTAAGCTTCTTGGAAA+AGG	+	chr3.1:63436004-63436023	MS.gene70668:CDS	30.0%
	AACTGAGTAACATCAATCAC+AGG	-	chr3.1:63435980-63435999	None:intergenic	35.0%
	AAGTATCATCCAGGTTACTT+CGG	+	chr3.1:63435827-63435846	MS.gene70668:CDS	35.0%
	ATCTTGTCGATGTTGACAAT+AGG	-	chr3.1:63435902-63435921	None:intergenic	35.0%
	GATTGCTGAAAAGAAGATCA+AGG	+	chr3.1:63436078-63436097	MS.gene70668:CDS	35.0%
	GTGATTGATGTTACTCAGTT+TGG	+	chr3.1:63435980-63435999	MS.gene70668:CDS	35.0%
	TCTTGTCGATGTTGACAATA+GGG	-	chr3.1:63435901-63435920	None:intergenic	35.0%
!	TTTGGGTTCTTTAAGCTTCT+TGG	+	chr3.1:63435998-63436017	MS.gene70668:CDS	35.0%
!!	AGCTTAGTCTTGACAACAAA+AGG	-	chr3.1:63436049-63436068	None:intergenic	35.0%
	AAGAAGAACAGGAAGAAGAG+AGG	+	chr3.1:63435704-63435723	MS.gene70668:CDS	40.0%
	GAAAAGAAGATCAAGGAAGC+TGG	+	chr3.1:63436085-63436104	MS.gene70668:CDS	40.0%
	GATACTTGTCGAAGAGGATA+CGG	-	chr3.1:63435814-63435833	None:intergenic	40.0%
	TCTCATTCCTCAAGATGTGA+AGG	+	chr3.1:63435928-63435947	MS.gene70668:CDS	40.0%
	TCTTCCTGTTCTTCTTGAAG+CGG	-	chr3.1:63435700-63435719	None:intergenic	40.0%
!	ATCTTCGTGAGGTTTTGCTT+TGG	-	chr3.1:63435671-63435690	None:intergenic	40.0%
!	TAGTCTTGACAACAAAAGGC+TGG	-	chr3.1:63436045-63436064	None:intergenic	40.0%
!	TGTGAAGGAGAAAGCTTTGA+AGG	+	chr3.1:63435943-63435962	MS.gene70668:CDS	40.0%
	AAGAAGATCAAGGAAGCTGG+TGG	+	chr3.1:63436088-63436107	MS.gene70668:CDS	45.0%
	CTCTTCGACAAGTATCATCC+AGG	+	chr3.1:63435818-63435837	MS.gene70668:CDS	45.0%
	CTGGATGATACTTGTCGAAG+AGG	-	chr3.1:63435820-63435839	None:intergenic	45.0%
	CTGTTGTTCTCACTGCTTGA+GGG	+	chr3.1:63436113-63436132	MS.gene70668:CDS	45.0%
	CTTCCTGTTCTTCTTGAAGC+GGG	-	chr3.1:63435699-63435718	None:intergenic	45.0%
	GCTTTCTCCTTCACATCTTG+AGG	-	chr3.1:63435938-63435957	None:intergenic	45.0%
	TCATCCAGGTTACTTCGGTA+AGG	+	chr3.1:63435832-63435851	MS.gene70668:CDS	45.0%
!!	ACAACAAAAGGCTGGTTCTG+AGG	-	chr3.1:63436037-63436056	None:intergenic	45.0%
!!	GTGAGGTTTTGCTTTGGATG+CGG	-	chr3.1:63435665-63435684	None:intergenic	45.0%
	CCAACCTTACCGAAGTAACC+TGG	-	chr3.1:63435839-63435858	None:intergenic	50.0%
	CCAGGTTACTTCGGTAAGGT+TGG	+	chr3.1:63435836-63435855	MS.gene70668:CDS	50.0%
	CTTTCCGATACGACCATGAC+CGG	-	chr3.1:63435741-63435760	None:intergenic	50.0%
	GCTGTTGTTCTCACTGCTTG+AGG	+	chr3.1:63436112-63436131	MS.gene70668:CDS	50.0%
	TGTCGAAGAGGATACGGTGA+TGG	-	chr3.1:63435808-63435827	None:intergenic	50.0%
	CGACCCGCTTCAAGAAGAAC+AGG	+	chr3.1:63435693-63435712	MS.gene70668:CDS	55.0%
!	ACGGTGATGGTGCATTCCTC+CGG	-	chr3.1:63435795-63435814	None:intergenic	55.0%
!!	GGAAAGCATCGCAAGCATCC+CGG	+	chr3.1:63435755-63435774	MS.gene70668:CDS	55.0%
	AAGAGAGGTCACGTGAGCGC+CGG	+	chr3.1:63435719-63435738	MS.gene70668:CDS	60.0%
	AGCGGGTCGTCATCTTCGTG+AGG	-	chr3.1:63435682-63435701	None:intergenic	60.0%
!	AAGCATCGCAAGCATCCCGG+AGG	+	chr3.1:63435758-63435777	MS.gene70668:CDS	60.0%
!!	AGCGCCGGTCATGGTCGTAT+CGG	+	chr3.1:63435734-63435753	MS.gene70668:CDS	60.0%
	TCCGGCGTTACCACGACCTC+CGG	-	chr3.1:63435777-63435796	None:intergenic	65.0%
	CCGGCGTTACCACGACCTCC+GGG	-	chr3.1:63435776-63435795	None:intergenic	70.0%
	CGCAAGCATCCCGGAGGTCG+TGG	+	chr3.1:63435764-63435783	MS.gene70668:CDS	70.0%
!	CCCGGAGGTCGTGGTAACGC+CGG	+	chr3.1:63435773-63435792	MS.gene70668:CDS	70.0%
!	GGAGGTCGTGGTAACGCCGG+AGG	+	chr3.1:63435776-63435795	MS.gene70668:CDS	70.0%
!!	GGTCACGTGAGCGCCGGTCA+TGG	+	chr3.1:63435725-63435744	MS.gene70668:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	63435650	63436132	63435650	ID=MS.gene70668
chr3.1	mRNA	63435650	63436132	63435650	ID=MS.gene70668.t1;Parent=MS.gene70668
chr3.1	exon	63435650	63436132	63435650	ID=MS.gene70668.t1.exon1;Parent=MS.gene70668.t1
chr3.1	CDS	63435650	63436132	63435650	ID=cds.MS.gene70668.t1;Parent=MS.gene70668.t1

Gene Sequence

>MS.gene70668

GGGCAAGCAAAGCCGCATCCAAAGCAAAACCTCACGAAGATGACGACCCGCTTCAAGAAGAACAGGAAGAAGAGAGGTCACGTGAGCGCCGGTCATGGTCGTATCGGAAAGCATCGCAAGCATCCCGGAGGTCGTGGTAACGCCGGAGGAATGCACCATCACCGTATCCTCTTCGACAAGTATCATCCAGGTTACTTCGGTAAGGTTGGTATGCGTTACTTTCACAAGCTTCGTAACAAATTCTATAACCCTATTGTCAACATCGACAAGATCGCTTCTCTCATTCCTCAAGATGTGAAGGAGAAAGCTTTGAAGGAGAATAAAGCTCCTGTGATTGATGTTACTCAGTTTGGGTTCTTTAAGCTTCTTGGAAAAGGTGTTTTGCCTCAGAACCAGCCTTTTGTTGTCAAGACTAAGCTGATTTCGAAGATTGCTGAAAAGAAGATCAAGGAAGCTGGTGGTGCTGTTGTTCTCACTGCTTGA

Protein sequence

>MS.gene70668.t1

GQAKPHPKQNLTKMTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQPFVVKTKLISKIAEKKIKEAGGAVVLTA