AlfalfaGEDB | MS.gene26005

M. sativa cultivar XinJiangDaYe / MS.gene26005

KEGG: K02900
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00828.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26005.t1	XP_003613127.2	100	147	0	0	1	147	1	147	9.30E-52	213

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26005.t1	P49637	77.6	147	32	1	1	147	1	146	6.1e-44	178.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26005.t1	G7JYX5	100.0	147	0	0	1	147	1	147	6.7e-52	213.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049387	MS.gene26005	0.821716	3.48E-53	-1.69E-46
MS.gene052666	MS.gene26005	0.817991	2.47E-52	-1.69E-46
MS.gene057641	MS.gene26005	0.821899	3.16E-53	-1.69E-46
MS.gene057643	MS.gene26005	0.822228	2.65E-53	-1.69E-46
MS.gene059340	MS.gene26005	0.839324	1.70E-57	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene26005	MS.gene74837	PPI
MS.gene022341	MS.gene26005	PPI
MS.gene26005	MS.gene022341	PPI
MS.gene70668	MS.gene26005	PPI
MS.gene26005	MS.gene86214	PPI
MS.gene26005	MS.gene29174	PPI
MS.gene26005	MS.gene44297	PPI
MS.gene26005	MS.gene027718	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26005.t1	MTR_5g033090	100.000	147	0	0	1	147	1	147	2.98e-104	294
MS.gene26005.t1	MTR_3g077050	99.320	147	1	0	1	147	1	147	7.09e-104	293
MS.gene26005.t1	MTR_8g069460	84.459	148	22	1	1	147	1	148	8.14e-88	253
MS.gene26005.t1	MTR_4g119380	53.741	147	14	3	1	147	100	192	3.35e-36	124

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26005.t1	AT1G70600	85.714	147	20	1	1	147	1	146	9.47e-89	255
MS.gene26005.t1	AT1G23290	83.673	147	23	1	1	147	1	146	3.32e-86	248
MS.gene26005.t1	AT1G12960	50.000	146	27	2	1	146	1	100	1.75e-36	121

Find 40 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGGGTTCTTTAAGCTTCT+TGG	0.217196	3.2:+68196334	MS.gene26005:CDS
GTGATTGATGTTACTCAGTT+TGG	0.327888	3.2:+68196316	MS.gene26005:CDS
TGATTGATGTTACTCAGTTT+GGG	0.380454	3.2:+68196317	MS.gene26005:CDS
TTCTTTAAGCTTCTTGGAAA+AGG	0.391337	3.2:+68196340	MS.gene26005:CDS
TGTGAAGGAGAAAGCTTTGA+AGG	0.400500	3.2:+68196279	MS.gene26005:CDS
ATCTTGTCGATGTTGACAAT+AGG	0.419901	3.2:-68196235	None:intergenic
TCTTGTCGATGTTGACAATA+GGG	0.425312	3.2:-68196234	None:intergenic
GCTTTCTCCTTCACATCTTG+TGG	0.427470	3.2:-68196271	None:intergenic
AGCTTAGTCTTGACAACAAA+AGG	0.442858	3.2:-68196382	None:intergenic
TCCGGCGTTACCACGACCTC+CGG	0.443108	3.2:-68196110	None:intergenic
CGCAAGCATCCCGGAGGTCG+TGG	0.447863	3.2:+68196100	MS.gene26005:CDS
CTTCCTGTTCTTCTTGAAGC+GGG	0.447997	3.2:-68196032	None:intergenic
CGACCCGCTTCAAGAAGAAC+AGG	0.481196	3.2:+68196029	MS.gene26005:CDS
ACGGTGATGGTGCATTCCTC+CGG	0.496860	3.2:-68196128	None:intergenic
CCAACCTTACCGAAGTAACC+TGG	0.506573	3.2:-68196172	None:intergenic
GATACTTGTCGAAGAGGATA+CGG	0.513304	3.2:-68196147	None:intergenic
CCAGGTTACTTCGGTAAGGT+TGG	0.536880	3.2:+68196172	MS.gene26005:CDS
TCTTCCTGTTCTTCTTGAAG+CGG	0.538920	3.2:-68196033	None:intergenic
GGTCACGTGAGCGCCGGTCA+TGG	0.538972	3.2:+68196061	MS.gene26005:CDS
TAGTCTTGACAACAAAAGGC+TGG	0.539190	3.2:-68196378	None:intergenic
GATTGCTGAAAAGAAGATCA+AGG	0.548421	3.2:+68196414	MS.gene26005:CDS
TCATCCAGGTTACTTCGGTA+AGG	0.549171	3.2:+68196168	MS.gene26005:CDS
CTCTTCGACAAGTATCATCC+AGG	0.553418	3.2:+68196154	MS.gene26005:CDS
CTTTCCGATACGACCATGAC+CGG	0.562794	3.2:-68196074	None:intergenic
ACAACAAAAGGCTGGTTCTG+AGG	0.563935	3.2:-68196370	None:intergenic
TCTCATTCCACAAGATGTGA+AGG	0.564862	3.2:+68196264	MS.gene26005:CDS
GAAAAGAAGATCAAGGAAGC+TGG	0.567856	3.2:+68196421	MS.gene26005:CDS
CCCGGAGGTCGTGGTAACGC+CGG	0.572163	3.2:+68196109	MS.gene26005:CDS
AAGAGAGGTCACGTGAGCGC+CGG	0.573118	3.2:+68196055	MS.gene26005:CDS
CCGGCGTTACCACGACCTCC+GGG	0.573655	3.2:-68196109	None:intergenic
AGCGCCGGTCATGGTCGTAT+CGG	0.577785	3.2:+68196070	MS.gene26005:CDS
AAGTATCATCCAGGTTACTT+CGG	0.578842	3.2:+68196163	MS.gene26005:CDS
GGAAAGCATCGCAAGCATCC+CGG	0.586004	3.2:+68196091	MS.gene26005:CDS
AAGAAGAACAGGAAGAAGAG+AGG	0.594785	3.2:+68196040	MS.gene26005:CDS
CTGGATGATACTTGTCGAAG+AGG	0.597192	3.2:-68196153	None:intergenic
AACTGAGTAACATCAATCAC+AGG	0.611236	3.2:-68196313	None:intergenic
GGAGGTCGTGGTAACGCCGG+AGG	0.620157	3.2:+68196112	MS.gene26005:CDS
TGTCGAAGAGGATACGGTGA+TGG	0.642158	3.2:-68196141	None:intergenic
AAGAAGATCAAGGAAGCTGG+TGG	0.687960	3.2:+68196424	MS.gene26005:CDS
AAGCATCGCAAGCATCCCGG+AGG	0.718770	3.2:+68196094	MS.gene26005:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TGATTGATGTTACTCAGTTT+GGG	+	chr3.2:68196317-68196336	MS.gene26005:CDS	30.0%
	TTCTTTAAGCTTCTTGGAAA+AGG	+	chr3.2:68196340-68196359	MS.gene26005:CDS	30.0%
	AACTGAGTAACATCAATCAC+AGG	-	chr3.2:68196316-68196335	None:intergenic	35.0%
	AAGTATCATCCAGGTTACTT+CGG	+	chr3.2:68196163-68196182	MS.gene26005:CDS	35.0%
	ATCTTGTCGATGTTGACAAT+AGG	-	chr3.2:68196238-68196257	None:intergenic	35.0%
	GATTGCTGAAAAGAAGATCA+AGG	+	chr3.2:68196414-68196433	MS.gene26005:CDS	35.0%
	GTGATTGATGTTACTCAGTT+TGG	+	chr3.2:68196316-68196335	MS.gene26005:CDS	35.0%
	TCTTGTCGATGTTGACAATA+GGG	-	chr3.2:68196237-68196256	None:intergenic	35.0%
!	TTTGGGTTCTTTAAGCTTCT+TGG	+	chr3.2:68196334-68196353	MS.gene26005:CDS	35.0%
!!	AGCTTAGTCTTGACAACAAA+AGG	-	chr3.2:68196385-68196404	None:intergenic	35.0%
	AAGAAGAACAGGAAGAAGAG+AGG	+	chr3.2:68196040-68196059	MS.gene26005:CDS	40.0%
	GAAAAGAAGATCAAGGAAGC+TGG	+	chr3.2:68196421-68196440	MS.gene26005:CDS	40.0%
	GATACTTGTCGAAGAGGATA+CGG	-	chr3.2:68196150-68196169	None:intergenic	40.0%
	TCTCATTCCACAAGATGTGA+AGG	+	chr3.2:68196264-68196283	MS.gene26005:CDS	40.0%
	TCTTCCTGTTCTTCTTGAAG+CGG	-	chr3.2:68196036-68196055	None:intergenic	40.0%
!	TAGTCTTGACAACAAAAGGC+TGG	-	chr3.2:68196381-68196400	None:intergenic	40.0%
!	TGTGAAGGAGAAAGCTTTGA+AGG	+	chr3.2:68196279-68196298	MS.gene26005:CDS	40.0%
	AAGAAGATCAAGGAAGCTGG+TGG	+	chr3.2:68196424-68196443	MS.gene26005:CDS	45.0%
	CTCTTCGACAAGTATCATCC+AGG	+	chr3.2:68196154-68196173	MS.gene26005:CDS	45.0%
	CTGGATGATACTTGTCGAAG+AGG	-	chr3.2:68196156-68196175	None:intergenic	45.0%
	CTTCCTGTTCTTCTTGAAGC+GGG	-	chr3.2:68196035-68196054	None:intergenic	45.0%
	GCTTTCTCCTTCACATCTTG+TGG	-	chr3.2:68196274-68196293	None:intergenic	45.0%
	TCATCCAGGTTACTTCGGTA+AGG	+	chr3.2:68196168-68196187	MS.gene26005:CDS	45.0%
!!	ACAACAAAAGGCTGGTTCTG+AGG	-	chr3.2:68196373-68196392	None:intergenic	45.0%
	CCAACCTTACCGAAGTAACC+TGG	-	chr3.2:68196175-68196194	None:intergenic	50.0%
	CCAGGTTACTTCGGTAAGGT+TGG	+	chr3.2:68196172-68196191	MS.gene26005:CDS	50.0%
	CTTTCCGATACGACCATGAC+CGG	-	chr3.2:68196077-68196096	None:intergenic	50.0%
	TGTCGAAGAGGATACGGTGA+TGG	-	chr3.2:68196144-68196163	None:intergenic	50.0%
	CGACCCGCTTCAAGAAGAAC+AGG	+	chr3.2:68196029-68196048	MS.gene26005:CDS	55.0%
!	ACGGTGATGGTGCATTCCTC+CGG	-	chr3.2:68196131-68196150	None:intergenic	55.0%
!!	GGAAAGCATCGCAAGCATCC+CGG	+	chr3.2:68196091-68196110	MS.gene26005:CDS	55.0%
	AAGAGAGGTCACGTGAGCGC+CGG	+	chr3.2:68196055-68196074	MS.gene26005:CDS	60.0%
!	AAGCATCGCAAGCATCCCGG+AGG	+	chr3.2:68196094-68196113	MS.gene26005:CDS	60.0%
!!	AGCGCCGGTCATGGTCGTAT+CGG	+	chr3.2:68196070-68196089	MS.gene26005:CDS	60.0%
	TCCGGCGTTACCACGACCTC+CGG	-	chr3.2:68196113-68196132	None:intergenic	65.0%
	CCGGCGTTACCACGACCTCC+GGG	-	chr3.2:68196112-68196131	None:intergenic	70.0%
	CGCAAGCATCCCGGAGGTCG+TGG	+	chr3.2:68196100-68196119	MS.gene26005:CDS	70.0%
!	CCCGGAGGTCGTGGTAACGC+CGG	+	chr3.2:68196109-68196128	MS.gene26005:CDS	70.0%
!	GGAGGTCGTGGTAACGCCGG+AGG	+	chr3.2:68196112-68196131	MS.gene26005:CDS	70.0%
!!	GGTCACGTGAGCGCCGGTCA+TGG	+	chr3.2:68196061-68196080	MS.gene26005:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	68196025	68196468	68196025	ID=MS.gene26005
chr3.2	mRNA	68196025	68196468	68196025	ID=MS.gene26005.t1;Parent=MS.gene26005
chr3.2	exon	68196025	68196468	68196025	ID=MS.gene26005.t1.exon1;Parent=MS.gene26005.t1
chr3.2	CDS	68196025	68196468	68196025	ID=cds.MS.gene26005.t1;Parent=MS.gene26005.t1

Gene Sequence

>MS.gene26005

ATGACGACCCGCTTCAAGAAGAACAGGAAGAAGAGAGGTCACGTGAGCGCCGGTCATGGTCGTATCGGAAAGCATCGCAAGCATCCCGGAGGTCGTGGTAACGCCGGAGGAATGCACCATCACCGTATCCTCTTCGACAAGTATCATCCAGGTTACTTCGGTAAGGTTGGTATGCGTTACTTTCACAAGCTTCGTAACAAATTCTATAACCCTATTGTCAACATCGACAAGATCGCTTCTCTCATTCCACAAGATGTGAAGGAGAAAGCTTTGAAGGAGAATAAAGCTCCTGTGATTGATGTTACTCAGTTTGGGTTCTTTAAGCTTCTTGGAAAAGGTGTTTTGCCTCAGAACCAGCCTTTTGTTGTCAAGACTAAGCTGATTTCGAAGATTGCTGAAAAGAAGATCAAGGAAGCTGGTGGTGCTGTTGTTCTTACTGCTTGA

Protein sequence

>MS.gene26005.t1

MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQPFVVKTKLISKIAEKKIKEAGGAVVLTA