AlfalfaGEDB | MS.gene86214

M. sativa cultivar XinJiangDaYe / MS.gene86214

KEGG: K02900
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00828.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86214.t1	XP_003613127.2	100	147	0	0	1	147	1	147	9.30E-52	213

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86214.t1	P49637	77.6	147	32	1	1	147	1	146	6.1e-44	178.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86214.t1	G7JYX5	100.0	147	0	0	1	147	1	147	6.7e-52	213.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053952	MS.gene86214	0.81923	1.30E-52	-1.69E-46
MS.gene061145	MS.gene86214	0.804803	1.81E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene70668	MS.gene86214	PPI
MS.gene74837	MS.gene86214	PPI
MS.gene86214	MS.gene74837	PPI
MS.gene027718	MS.gene86214	PPI
MS.gene44297	MS.gene86214	PPI
MS.gene26005	MS.gene86214	PPI
MS.gene29174	MS.gene86214	PPI
MS.gene022341	MS.gene86214	PPI
MS.gene86214	MS.gene29174	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86214.t1	MTR_5g033090	100.000	147	0	0	1	147	1	147	2.98e-104	294
MS.gene86214.t1	MTR_3g077050	99.320	147	1	0	1	147	1	147	7.09e-104	293
MS.gene86214.t1	MTR_8g069460	84.459	148	22	1	1	147	1	148	8.14e-88	253
MS.gene86214.t1	MTR_4g119380	53.741	147	14	3	1	147	100	192	3.35e-36	124

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86214.t1	AT1G70600	85.714	147	20	1	1	147	1	146	9.47e-89	255
MS.gene86214.t1	AT1G23290	83.673	147	23	1	1	147	1	146	3.32e-86	248
MS.gene86214.t1	AT1G12960	50.000	146	27	2	1	146	1	100	1.75e-36	121

Find 45 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGATTGATGTTACTCAGTT+TGG	0.310495	5.1:+23598745	MS.gene86214:CDS
TCTTCCTGTTCTTCTTGAAT+CGG	0.319606	5.1:-23598462	None:intergenic
TTTGATTCCACAAGATGTTA+AGG	0.375048	5.1:+23598693	MS.gene86214:CDS
TGTTAAGGAGAAGGCTTTGA+AGG	0.376961	5.1:+23598708	MS.gene86214:CDS
TGATTGATGTTACTCAGTTT+GGG	0.380111	5.1:+23598746	MS.gene86214:CDS
GATTGCGGAGAAGAAGATTA+AGG	0.381172	5.1:+23598843	MS.gene86214:CDS
CTTCCTGTTCTTCTTGAATC+GGG	0.382353	5.1:-23598461	None:intergenic
ATCTTGTCGATGTTGACAAT+GGG	0.394209	5.1:-23598664	None:intergenic
TCCGGCGTTACCACGACCTC+CGG	0.443108	5.1:-23598539	None:intergenic
CGCAAGCATCCCGGAGGTCG+TGG	0.447863	5.1:+23598529	MS.gene86214:CDS
GCCTTCTCCTTAACATCTTG+TGG	0.452270	5.1:-23598700	None:intergenic
TGCTGTTGTTCTTACTGCTT+AGG	0.456887	5.1:+23598876	MS.gene86214:CDS
CTTTCCGATACGACCGTGTC+CGG	0.463430	5.1:-23598503	None:intergenic
AGCTTCGTCTTCACAACAAA+AGG	0.468189	5.1:-23598811	None:intergenic
CCAGGTTACTTCGGTAAAGT+TGG	0.475314	5.1:+23598601	MS.gene86214:CDS
CAACCCGATTCAAGAAGAAC+AGG	0.488410	5.1:+23598458	MS.gene86214:CDS
AAGCGCGGCCACGTCAGCGC+CGG	0.506311	5.1:+23598484	MS.gene86214:CDS
GGCCACGTCAGCGCCGGACA+CGG	0.507873	5.1:+23598490	MS.gene86214:CDS
CCAACTTTACCGAAGTAACC+TGG	0.507906	5.1:-23598601	None:intergenic
GACCGTGTCCGGCGCTGACG+TGG	0.514645	5.1:-23598492	None:intergenic
AGCGCCGGACACGGTCGTAT+CGG	0.530105	5.1:+23598499	MS.gene86214:CDS
TCCACAAGATGTTAAGGAGA+AGG	0.532638	5.1:+23598699	MS.gene86214:CDS
CTCTTCGACAAGTACCATCC+AGG	0.549117	5.1:+23598583	MS.gene86214:CDS
CACGACCTCCGGGATGCTTG+CGG	0.564581	5.1:-23598528	None:intergenic
CCCGGAGGTCGTGGTAACGC+CGG	0.572163	5.1:+23598538	MS.gene86214:CDS
GGTACTTGTCGAAGAGGATG+CGG	0.572674	5.1:-23598576	None:intergenic
GGAAAGCACCGCAAGCATCC+CGG	0.572998	5.1:+23598520	MS.gene86214:CDS
GAGAAGAAGATTAAGGAGGC+TGG	0.580387	5.1:+23598850	MS.gene86214:CDS
GATCTTGTCGATGTTGACAA+TGG	0.583929	5.1:-23598665	None:intergenic
CCGGCGTTACCACGACCTCC+GGG	0.586499	5.1:-23598538	None:intergenic
AAGTACCATCCAGGTTACTT+CGG	0.598039	5.1:+23598592	MS.gene86214:CDS
GAAGCTGATTTCGAAGATTG+CGG	0.600062	5.1:+23598828	MS.gene86214:CDS
GCGGTGGTGATGCATACCTC+CGG	0.601360	5.1:-23598557	None:intergenic
TGGGGTTGTAGAATTTGTTG+CGG	0.602278	5.1:-23598645	None:intergenic
CTGGATGGTACTTGTCGAAG+AGG	0.607823	5.1:-23598582	None:intergenic
GGAGGTCGTGGTAACGCCGG+AGG	0.608528	5.1:+23598541	MS.gene86214:CDS
AACTGAGTAACATCAATCAC+CGG	0.615568	5.1:-23598742	None:intergenic
TTTGAAGGAGAACAAAGCGC+CGG	0.644488	5.1:+23598723	MS.gene86214:CDS
CTTTACCGAAGTAACCTGGA+TGG	0.684945	5.1:-23598597	None:intergenic
AAGAAGATTAAGGAGGCTGG+TGG	0.689384	5.1:+23598853	MS.gene86214:CDS
AAGAAGAACAGGAAGAAGCG+CGG	0.691929	5.1:+23598469	MS.gene86214:CDS
TGCGGAGAAGAAGATTAAGG+AGG	0.697175	5.1:+23598846	MS.gene86214:CDS
ACTTGTCGAAGAGGATGCGG+TGG	0.709791	5.1:-23598573	None:intergenic
TCTTGTCGATGTTGACAATG+GGG	0.721275	5.1:-23598663	None:intergenic
AAGCACCGCAAGCATCCCGG+AGG	0.729195	5.1:+23598523	MS.gene86214:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTGGGTTTTTTAAGCTTTTA+GGG	+	chr5.1:23598764-23598783	MS.gene86214:CDS	25.0%
!!!	TTTGGGTTTTTTAAGCTTTT+AGG	+	chr5.1:23598763-23598782	MS.gene86214:CDS	25.0%
!!!	TTTTTTAAGCTTTTAGGGAA+AGG	+	chr5.1:23598769-23598788	MS.gene86214:CDS	25.0%
	TGATTGATGTTACTCAGTTT+GGG	+	chr5.1:23598746-23598765	MS.gene86214:CDS	30.0%
	TTTGATTCCACAAGATGTTA+AGG	+	chr5.1:23598693-23598712	MS.gene86214:CDS	30.0%
	AACTGAGTAACATCAATCAC+CGG	-	chr5.1:23598745-23598764	None:intergenic	35.0%
	ATCTTGTCGATGTTGACAAT+GGG	-	chr5.1:23598667-23598686	None:intergenic	35.0%
	GTGATTGATGTTACTCAGTT+TGG	+	chr5.1:23598745-23598764	MS.gene86214:CDS	35.0%
	TCTTCCTGTTCTTCTTGAAT+CGG	-	chr5.1:23598465-23598484	None:intergenic	35.0%
!!	ACAACAAAAGGCTGATTTTG+CGG	-	chr5.1:23598802-23598821	None:intergenic	35.0%
	AAGTACCATCCAGGTTACTT+CGG	+	chr5.1:23598592-23598611	MS.gene86214:CDS	40.0%
	AGCTTCGTCTTCACAACAAA+AGG	-	chr5.1:23598814-23598833	None:intergenic	40.0%
	CTTCCTGTTCTTCTTGAATC+GGG	-	chr5.1:23598464-23598483	None:intergenic	40.0%
	GATCTTGTCGATGTTGACAA+TGG	-	chr5.1:23598668-23598687	None:intergenic	40.0%
	GATTGCGGAGAAGAAGATTA+AGG	+	chr5.1:23598843-23598862	MS.gene86214:CDS	40.0%
	TCCACAAGATGTTAAGGAGA+AGG	+	chr5.1:23598699-23598718	MS.gene86214:CDS	40.0%
	TCTTGTCGATGTTGACAATG+GGG	-	chr5.1:23598666-23598685	None:intergenic	40.0%
!	GAAGCTGATTTCGAAGATTG+CGG	+	chr5.1:23598828-23598847	MS.gene86214:CDS	40.0%
!	TGTTAAGGAGAAGGCTTTGA+AGG	+	chr5.1:23598708-23598727	MS.gene86214:CDS	40.0%
!!	TGGGGTTGTAGAATTTGTTG+CGG	-	chr5.1:23598648-23598667	None:intergenic	40.0%
	AAGAAGAACAGGAAGAAGCG+CGG	+	chr5.1:23598469-23598488	MS.gene86214:CDS	45.0%
	AAGAAGATTAAGGAGGCTGG+TGG	+	chr5.1:23598853-23598872	MS.gene86214:CDS	45.0%
	CAACCCGATTCAAGAAGAAC+AGG	+	chr5.1:23598458-23598477	MS.gene86214:CDS	45.0%
	CCAACTTTACCGAAGTAACC+TGG	-	chr5.1:23598604-23598623	None:intergenic	45.0%
	CCAGGTTACTTCGGTAAAGT+TGG	+	chr5.1:23598601-23598620	MS.gene86214:CDS	45.0%
	CTTTACCGAAGTAACCTGGA+TGG	-	chr5.1:23598600-23598619	None:intergenic	45.0%
	GAGAAGAAGATTAAGGAGGC+TGG	+	chr5.1:23598850-23598869	MS.gene86214:CDS	45.0%
	GCCTTCTCCTTAACATCTTG+TGG	-	chr5.1:23598703-23598722	None:intergenic	45.0%
	TGCGGAGAAGAAGATTAAGG+AGG	+	chr5.1:23598846-23598865	MS.gene86214:CDS	45.0%
	TTTGAAGGAGAACAAAGCGC+CGG	+	chr5.1:23598723-23598742	MS.gene86214:CDS	45.0%
	CTCTTCGACAAGTACCATCC+AGG	+	chr5.1:23598583-23598602	MS.gene86214:CDS	50.0%
	CTGGATGGTACTTGTCGAAG+AGG	-	chr5.1:23598585-23598604	None:intergenic	50.0%
	GGTACTTGTCGAAGAGGATG+CGG	-	chr5.1:23598579-23598598	None:intergenic	50.0%
	ACTTGTCGAAGAGGATGCGG+TGG	-	chr5.1:23598576-23598595	None:intergenic	55.0%
	CTTTCCGATACGACCGTGTC+CGG	-	chr5.1:23598506-23598525	None:intergenic	55.0%
!	GCGGTGGTGATGCATACCTC+CGG	-	chr5.1:23598560-23598579	None:intergenic	60.0%
!!	GGAAAGCACCGCAAGCATCC+CGG	+	chr5.1:23598520-23598539	MS.gene86214:CDS	60.0%
	CACGACCTCCGGGATGCTTG+CGG	-	chr5.1:23598531-23598550	None:intergenic	65.0%
	TCCGGCGTTACCACGACCTC+CGG	-	chr5.1:23598542-23598561	None:intergenic	65.0%
!	AAGCACCGCAAGCATCCCGG+AGG	+	chr5.1:23598523-23598542	MS.gene86214:CDS	65.0%
!	AGCGCCGGACACGGTCGTAT+CGG	+	chr5.1:23598499-23598518	MS.gene86214:CDS	65.0%
	CCGGCGTTACCACGACCTCC+GGG	-	chr5.1:23598541-23598560	None:intergenic	70.0%
	CGCAAGCATCCCGGAGGTCG+TGG	+	chr5.1:23598529-23598548	MS.gene86214:CDS	70.0%
!	CCCGGAGGTCGTGGTAACGC+CGG	+	chr5.1:23598538-23598557	MS.gene86214:CDS	70.0%
!	GGAGGTCGTGGTAACGCCGG+AGG	+	chr5.1:23598541-23598560	MS.gene86214:CDS	70.0%
	AAGCGCGGCCACGTCAGCGC+CGG	+	chr5.1:23598484-23598503	MS.gene86214:CDS	75.0%
!	GGCCACGTCAGCGCCGGACA+CGG	+	chr5.1:23598490-23598509	MS.gene86214:CDS	75.0%
!!	GACCGTGTCCGGCGCTGACG+TGG	-	chr5.1:23598495-23598514	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	23598454	23598897	23598454	ID=MS.gene86214
chr5.1	mRNA	23598454	23598897	23598454	ID=MS.gene86214.t1;Parent=MS.gene86214
chr5.1	exon	23598454	23598897	23598454	ID=MS.gene86214.t1.exon1;Parent=MS.gene86214.t1
chr5.1	CDS	23598454	23598897	23598454	ID=cds.MS.gene86214.t1;Parent=MS.gene86214.t1

Gene Sequence

>MS.gene86214

ATGACAACCCGATTCAAGAAGAACAGGAAGAAGCGCGGCCACGTCAGCGCCGGACACGGTCGTATCGGAAAGCACCGCAAGCATCCCGGAGGTCGTGGTAACGCCGGAGGTATGCATCACCACCGCATCCTCTTCGACAAGTACCATCCAGGTTACTTCGGTAAAGTTGGTATGCGTTACTTTCACAAGCTCCGCAACAAATTCTACAACCCCATTGTCAACATCGACAAGATCGCTTCTTTGATTCCACAAGATGTTAAGGAGAAGGCTTTGAAGGAGAACAAAGCGCCGGTGATTGATGTTACTCAGTTTGGGTTTTTTAAGCTTTTAGGGAAAGGTGTTTTGCCGCAAAATCAGCCTTTTGTTGTGAAGACGAAGCTGATTTCGAAGATTGCGGAGAAGAAGATTAAGGAGGCTGGTGGTGCTGTTGTTCTTACTGCTTAG

Protein sequence

>MS.gene86214.t1

MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQPFVVKTKLISKIAEKKIKEAGGAVVLTA