AlfalfaGEDB | MS.gene44297

M. sativa cultivar XinJiangDaYe / MS.gene44297

KEGG: K02900
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00828.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44297.t1	XP_003613127.2	100	147	0	0	1	147	1	147	9.30E-52	213

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44297.t1	P49637	77.6	147	32	1	1	147	1	146	6.1e-44	178.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44297.t1	G7JYX5	100.0	147	0	0	1	147	1	147	6.7e-52	213.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049387	MS.gene44297	0.806647	7.43E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene022341	MS.gene44297	PPI
MS.gene70668	MS.gene44297	PPI
MS.gene44297	MS.gene29174	PPI
MS.gene44297	MS.gene86214	PPI
MS.gene44297	MS.gene027718	PPI
MS.gene26005	MS.gene44297	PPI
MS.gene44297	MS.gene74837	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44297.t1	MTR_5g033090	100.000	147	0	0	1	147	1	147	2.98e-104	294
MS.gene44297.t1	MTR_3g077050	99.320	147	1	0	1	147	1	147	7.09e-104	293
MS.gene44297.t1	MTR_8g069460	84.459	148	22	1	1	147	1	148	8.14e-88	253
MS.gene44297.t1	MTR_4g119380	53.741	147	14	3	1	147	100	192	3.35e-36	124

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44297.t1	AT1G70600	85.714	147	20	1	1	147	1	146	9.47e-89	255
MS.gene44297.t1	AT1G23290	83.673	147	23	1	1	147	1	146	3.32e-86	248
MS.gene44297.t1	AT1G12960	50.000	146	27	2	1	146	1	100	1.75e-36	121

Find 47 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGATTGATGTTACTCAGTT+TGG	0.310495	5.4:+23218314	MS.gene44297:CDS
TCTTCCTGTTCTTCTTGAAT+CGG	0.319606	5.4:-23218031	None:intergenic
TTTGATTCCACAAGATGTTA+AGG	0.375048	5.4:+23218262	MS.gene44297:CDS
TGTTAAGGAGAAGGCTTTGA+AGG	0.376961	5.4:+23218277	MS.gene44297:CDS
TGATTGATGTTACTCAGTTT+GGG	0.380111	5.4:+23218315	MS.gene44297:CDS
GATTGCGGAGAAGAAGATTA+AGG	0.381172	5.4:+23218412	MS.gene44297:CDS
CTTCCTGTTCTTCTTGAATC+GGG	0.382353	5.4:-23218030	None:intergenic
ATCTTGTCGATGTTGACAAT+GGG	0.394209	5.4:-23218233	None:intergenic
TCCGGCGTTACCACGACCTC+CGG	0.443108	5.4:-23218108	None:intergenic
CGCAAGCATCCCGGAGGTCG+TGG	0.447863	5.4:+23218098	MS.gene44297:CDS
GCCTTCTCCTTAACATCTTG+TGG	0.452270	5.4:-23218269	None:intergenic
TGCTGTTGTTCTTACTGCTT+AGG	0.456887	5.4:+23218445	MS.gene44297:CDS
CTTTCCGATACGACCGTGTC+CGG	0.463430	5.4:-23218072	None:intergenic
AGCTTCGTCTTCACAACAAA+AGG	0.468189	5.4:-23218380	None:intergenic
TCCAGGTTACTTCGGTAAAG+TGG	0.475563	5.4:+23218169	MS.gene44297:CDS
CCCACTTTACCGAAGTAACC+TGG	0.480893	5.4:-23218170	None:intergenic
CAACCCGATTCAAGAAGAAC+AGG	0.488410	5.4:+23218027	MS.gene44297:CDS
TGGGGTTGTAGAATTTGTTA+CGG	0.498120	5.4:-23218214	None:intergenic
GACCGTGTCCGGCGCTGACG+TGG	0.514645	5.4:-23218061	None:intergenic
AGCGCCGGACACGGTCGTAT+CGG	0.530105	5.4:+23218068	MS.gene44297:CDS
AAGCGTGGCCACGTCAGCGC+CGG	0.530449	5.4:+23218053	MS.gene44297:CDS
TCCACAAGATGTTAAGGAGA+AGG	0.532638	5.4:+23218268	MS.gene44297:CDS
CTCTTCGACAAGTACCATCC+AGG	0.549117	5.4:+23218152	MS.gene44297:CDS
AGAATTTGTTACGGAGCTTG+TGG	0.563774	5.4:-23218205	None:intergenic
GGCCACGTCAGCGCCGGACA+CGG	0.564501	5.4:+23218059	MS.gene44297:CDS
CACGACCTCCGGGATGCTTG+CGG	0.564581	5.4:-23218097	None:intergenic
CCCGGAGGTCGTGGTAACGC+CGG	0.572163	5.4:+23218107	MS.gene44297:CDS
GGTACTTGTCGAAGAGGATG+CGG	0.572674	5.4:-23218145	None:intergenic
GGAAAGCACCGCAAGCATCC+CGG	0.572998	5.4:+23218089	MS.gene44297:CDS
GAGAAGAAGATTAAGGAGGC+TGG	0.580387	5.4:+23218419	MS.gene44297:CDS
GATCTTGTCGATGTTGACAA+TGG	0.583929	5.4:-23218234	None:intergenic
CCGGCGTTACCACGACCTCC+GGG	0.586499	5.4:-23218107	None:intergenic
CCAGGTTACTTCGGTAAAGT+GGG	0.594593	5.4:+23218170	MS.gene44297:CDS
AAGTACCATCCAGGTTACTT+CGG	0.598039	5.4:+23218161	MS.gene44297:CDS
GAAGCTGATTTCGAAGATTG+CGG	0.600062	5.4:+23218397	MS.gene44297:CDS
GCGGTGGTGATGCATACCTC+CGG	0.601360	5.4:-23218126	None:intergenic
CTGGATGGTACTTGTCGAAG+AGG	0.607823	5.4:-23218151	None:intergenic
GGAGGTCGTGGTAACGCCGG+AGG	0.608528	5.4:+23218110	MS.gene44297:CDS
AACTGAGTAACATCAATCAC+CGG	0.615568	5.4:-23218311	None:intergenic
TTTGAAGGAGAACAAAGCGC+CGG	0.644488	5.4:+23218292	MS.gene44297:CDS
CTTTACCGAAGTAACCTGGA+TGG	0.684945	5.4:-23218166	None:intergenic
AAGAAGAACAGGAAGAAGCG+TGG	0.686667	5.4:+23218038	MS.gene44297:CDS
AAGAAGATTAAGGAGGCTGG+TGG	0.689384	5.4:+23218422	MS.gene44297:CDS
TGCGGAGAAGAAGATTAAGG+AGG	0.697175	5.4:+23218415	MS.gene44297:CDS
ACTTGTCGAAGAGGATGCGG+TGG	0.709791	5.4:-23218142	None:intergenic
TCTTGTCGATGTTGACAATG+GGG	0.721275	5.4:-23218232	None:intergenic
AAGCACCGCAAGCATCCCGG+AGG	0.729195	5.4:+23218092	MS.gene44297:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTGGGTTTTTTAAGCTTTTA+GGG	+	chr5.4:23218333-23218352	MS.gene44297:CDS	25.0%
!!!	TTTGGGTTTTTTAAGCTTTT+AGG	+	chr5.4:23218332-23218351	MS.gene44297:CDS	25.0%
!!!	TTTTTTAAGCTTTTAGGGAA+AGG	+	chr5.4:23218338-23218357	MS.gene44297:CDS	25.0%
	TGATTGATGTTACTCAGTTT+GGG	+	chr5.4:23218315-23218334	MS.gene44297:CDS	30.0%
	TTTGATTCCACAAGATGTTA+AGG	+	chr5.4:23218262-23218281	MS.gene44297:CDS	30.0%
	AACTGAGTAACATCAATCAC+CGG	-	chr5.4:23218314-23218333	None:intergenic	35.0%
	ATCTTGTCGATGTTGACAAT+GGG	-	chr5.4:23218236-23218255	None:intergenic	35.0%
	GTGATTGATGTTACTCAGTT+TGG	+	chr5.4:23218314-23218333	MS.gene44297:CDS	35.0%
	TCTTCCTGTTCTTCTTGAAT+CGG	-	chr5.4:23218034-23218053	None:intergenic	35.0%
!!	ACAACAAAAGGCTGATTTTG+CGG	-	chr5.4:23218371-23218390	None:intergenic	35.0%
!!	TGGGGTTGTAGAATTTGTTA+CGG	-	chr5.4:23218217-23218236	None:intergenic	35.0%
	AAGTACCATCCAGGTTACTT+CGG	+	chr5.4:23218161-23218180	MS.gene44297:CDS	40.0%
	AGCTTCGTCTTCACAACAAA+AGG	-	chr5.4:23218383-23218402	None:intergenic	40.0%
	CTTCCTGTTCTTCTTGAATC+GGG	-	chr5.4:23218033-23218052	None:intergenic	40.0%
	GATCTTGTCGATGTTGACAA+TGG	-	chr5.4:23218237-23218256	None:intergenic	40.0%
	GATTGCGGAGAAGAAGATTA+AGG	+	chr5.4:23218412-23218431	MS.gene44297:CDS	40.0%
	TCCACAAGATGTTAAGGAGA+AGG	+	chr5.4:23218268-23218287	MS.gene44297:CDS	40.0%
	TCTTGTCGATGTTGACAATG+GGG	-	chr5.4:23218235-23218254	None:intergenic	40.0%
!	GAAGCTGATTTCGAAGATTG+CGG	+	chr5.4:23218397-23218416	MS.gene44297:CDS	40.0%
!	TGTTAAGGAGAAGGCTTTGA+AGG	+	chr5.4:23218277-23218296	MS.gene44297:CDS	40.0%
!!	AGAATTTGTTACGGAGCTTG+TGG	-	chr5.4:23218208-23218227	None:intergenic	40.0%
	AAGAAGAACAGGAAGAAGCG+TGG	+	chr5.4:23218038-23218057	MS.gene44297:CDS	45.0%
	AAGAAGATTAAGGAGGCTGG+TGG	+	chr5.4:23218422-23218441	MS.gene44297:CDS	45.0%
	CAACCCGATTCAAGAAGAAC+AGG	+	chr5.4:23218027-23218046	MS.gene44297:CDS	45.0%
	CCAGGTTACTTCGGTAAAGT+GGG	+	chr5.4:23218170-23218189	MS.gene44297:CDS	45.0%
	CTTTACCGAAGTAACCTGGA+TGG	-	chr5.4:23218169-23218188	None:intergenic	45.0%
	GAGAAGAAGATTAAGGAGGC+TGG	+	chr5.4:23218419-23218438	MS.gene44297:CDS	45.0%
	GCCTTCTCCTTAACATCTTG+TGG	-	chr5.4:23218272-23218291	None:intergenic	45.0%
	TCCAGGTTACTTCGGTAAAG+TGG	+	chr5.4:23218169-23218188	MS.gene44297:CDS	45.0%
	TGCGGAGAAGAAGATTAAGG+AGG	+	chr5.4:23218415-23218434	MS.gene44297:CDS	45.0%
	TTTGAAGGAGAACAAAGCGC+CGG	+	chr5.4:23218292-23218311	MS.gene44297:CDS	45.0%
	CCCACTTTACCGAAGTAACC+TGG	-	chr5.4:23218173-23218192	None:intergenic	50.0%
	CTCTTCGACAAGTACCATCC+AGG	+	chr5.4:23218152-23218171	MS.gene44297:CDS	50.0%
	CTGGATGGTACTTGTCGAAG+AGG	-	chr5.4:23218154-23218173	None:intergenic	50.0%
	GGTACTTGTCGAAGAGGATG+CGG	-	chr5.4:23218148-23218167	None:intergenic	50.0%
	ACTTGTCGAAGAGGATGCGG+TGG	-	chr5.4:23218145-23218164	None:intergenic	55.0%
	CTTTCCGATACGACCGTGTC+CGG	-	chr5.4:23218075-23218094	None:intergenic	55.0%
!	GCGGTGGTGATGCATACCTC+CGG	-	chr5.4:23218129-23218148	None:intergenic	60.0%
!!	GGAAAGCACCGCAAGCATCC+CGG	+	chr5.4:23218089-23218108	MS.gene44297:CDS	60.0%
	CACGACCTCCGGGATGCTTG+CGG	-	chr5.4:23218100-23218119	None:intergenic	65.0%
	TCCGGCGTTACCACGACCTC+CGG	-	chr5.4:23218111-23218130	None:intergenic	65.0%
!	AAGCACCGCAAGCATCCCGG+AGG	+	chr5.4:23218092-23218111	MS.gene44297:CDS	65.0%
!	AGCGCCGGACACGGTCGTAT+CGG	+	chr5.4:23218068-23218087	MS.gene44297:CDS	65.0%
	AAGCGTGGCCACGTCAGCGC+CGG	+	chr5.4:23218053-23218072	MS.gene44297:CDS	70.0%
	CCGGCGTTACCACGACCTCC+GGG	-	chr5.4:23218110-23218129	None:intergenic	70.0%
	CGCAAGCATCCCGGAGGTCG+TGG	+	chr5.4:23218098-23218117	MS.gene44297:CDS	70.0%
!	CCCGGAGGTCGTGGTAACGC+CGG	+	chr5.4:23218107-23218126	MS.gene44297:CDS	70.0%
!	GGAGGTCGTGGTAACGCCGG+AGG	+	chr5.4:23218110-23218129	MS.gene44297:CDS	70.0%
!	GGCCACGTCAGCGCCGGACA+CGG	+	chr5.4:23218059-23218078	MS.gene44297:CDS	75.0%
!!	GACCGTGTCCGGCGCTGACG+TGG	-	chr5.4:23218064-23218083	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	23218023	23218466	23218023	ID=MS.gene44297
chr5.4	mRNA	23218023	23218466	23218023	ID=MS.gene44297.t1;Parent=MS.gene44297
chr5.4	exon	23218023	23218466	23218023	ID=MS.gene44297.t1.exon1;Parent=MS.gene44297.t1
chr5.4	CDS	23218023	23218466	23218023	ID=cds.MS.gene44297.t1;Parent=MS.gene44297.t1

Gene Sequence

>MS.gene44297

ATGACAACCCGATTCAAGAAGAACAGGAAGAAGCGTGGCCACGTCAGCGCCGGACACGGTCGTATCGGAAAGCACCGCAAGCATCCCGGAGGTCGTGGTAACGCCGGAGGTATGCATCACCACCGCATCCTCTTCGACAAGTACCATCCAGGTTACTTCGGTAAAGTGGGTATGCGTTACTTCCACAAGCTCCGTAACAAATTCTACAACCCCATTGTCAACATCGACAAGATCGCTTCTTTGATTCCACAAGATGTTAAGGAGAAGGCTTTGAAGGAGAACAAAGCGCCGGTGATTGATGTTACTCAGTTTGGGTTTTTTAAGCTTTTAGGGAAAGGTGTTTTGCCGCAAAATCAGCCTTTTGTTGTGAAGACGAAGCTGATTTCGAAGATTGCGGAGAAGAAGATTAAGGAGGCTGGTGGTGCTGTTGTTCTTACTGCTTAG

Protein sequence

>MS.gene44297.t1

MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQPFVVKTKLISKIAEKKIKEAGGAVVLTA