AlfalfaGEDB | MS.gene027718

M. sativa cultivar XinJiangDaYe / MS.gene027718

KEGG: K02900
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00828.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027718.t1	XP_003613127.2	99.3	147	1	0	1	147	1	147	2.70E-51	211.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027718.t1	P49637	77.6	147	32	1	1	147	1	146	4.7e-44	178.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027718.t1	G7JYX5	99.3	147	1	0	1	147	1	147	2.0e-51	211.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene70668	MS.gene027718	PPI
MS.gene027718	MS.gene86214	PPI
MS.gene027718	MS.gene29174	PPI
MS.gene44297	MS.gene027718	PPI
MS.gene027718	MS.gene74837	PPI
MS.gene022341	MS.gene027718	PPI
MS.gene26005	MS.gene027718	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027718.t1	MTR_5g033090	99.320	147	1	0	1	147	1	147	1.63e-103	292
MS.gene027718.t1	MTR_3g077050	98.639	147	2	0	1	147	1	147	4.58e-103	291
MS.gene027718.t1	MTR_8g069460	83.784	148	23	1	1	147	1	148	4.61e-87	251
MS.gene027718.t1	MTR_4g119380	53.061	147	15	3	1	147	100	192	2.00e-35	122

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027718.t1	AT1G70600	85.714	147	20	1	1	147	1	146	1.19e-88	254
MS.gene027718.t1	AT1G23290	83.673	147	23	1	1	147	1	146	3.32e-86	248
MS.gene027718.t1	AT1G12960	50.000	146	27	2	1	146	1	100	1.71e-36	121

Find 43 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGATTGATGTTACTCAGTT+TGG	0.310495	5.2:+23146674	MS.gene027718:CDS
TCTTCCTGTTCTTCTTGAAT+CGG	0.319606	5.2:-23146391	None:intergenic
TTTGATTCCACAAGATGTTA+AGG	0.375048	5.2:+23146622	MS.gene027718:CDS
TGTTAAGGAGAAGGCTTTGA+AGG	0.376961	5.2:+23146637	MS.gene027718:CDS
TGATTGATGTTACTCAGTTT+GGG	0.380111	5.2:+23146675	MS.gene027718:CDS
GATTGCGGAGAAGAAGATTA+AGG	0.381172	5.2:+23146772	MS.gene027718:CDS
CTTCCTGTTCTTCTTGAATC+GGG	0.382353	5.2:-23146390	None:intergenic
ATCTTGTCGATGTTGACAAT+GGG	0.394209	5.2:-23146593	None:intergenic
TCCGGCGTTACCACGACCTC+CGG	0.443108	5.2:-23146468	None:intergenic
CGCAAGCATCCCGGAGGTCG+TGG	0.449325	5.2:+23146458	MS.gene027718:CDS
GCCTTCTCCTTAACATCTTG+TGG	0.452270	5.2:-23146629	None:intergenic
TGCTGTTGTTCTTACTGCTT+AGG	0.456887	5.2:+23146805	MS.gene027718:CDS
CCAGGTTACTTCGGTAAAGT+TGG	0.475314	5.2:+23146530	MS.gene027718:CDS
AGCTTCGTCTTCACAACAAA+AGG	0.482001	5.2:-23146740	None:intergenic
CAACCCGATTCAAGAAGAAC+AGG	0.488410	5.2:+23146387	MS.gene027718:CDS
AAGCGCGGCCACGTCAGCGC+CGG	0.506311	5.2:+23146413	MS.gene027718:CDS
GGCCACGTCAGCGCCGGACA+CGG	0.507873	5.2:+23146419	MS.gene027718:CDS
CCAACTTTACCGAAGTAACC+TGG	0.507906	5.2:-23146530	None:intergenic
GACCGTGTCCGGCGCTGACG+TGG	0.514645	5.2:-23146421	None:intergenic
GGTACTTGTCGAAGAGGATA+CGG	0.525231	5.2:-23146505	None:intergenic
AGCGCCGGACACGGTCGTAT+CGG	0.530105	5.2:+23146428	MS.gene027718:CDS
TCCACAAGATGTTAAGGAGA+AGG	0.532638	5.2:+23146628	MS.gene027718:CDS
CTCTTCGACAAGTACCATCC+AGG	0.545970	5.2:+23146512	MS.gene027718:CDS
CACGACCTCCGGGATGCTTG+CGG	0.564581	5.2:-23146457	None:intergenic
CCCGGAGGTCGTGGTAACGC+CGG	0.572163	5.2:+23146467	MS.gene027718:CDS
GAGAAGAAGATTAAGGAGGC+TGG	0.580387	5.2:+23146779	MS.gene027718:CDS
GATCTTGTCGATGTTGACAA+TGG	0.583929	5.2:-23146594	None:intergenic
CCGGCGTTACCACGACCTCC+GGG	0.586499	5.2:-23146467	None:intergenic
GGAAAACACCGCAAGCATCC+CGG	0.587466	5.2:+23146449	MS.gene027718:CDS
AAGTACCATCCAGGTTACTT+CGG	0.598039	5.2:+23146521	MS.gene027718:CDS
GAAGCTGATTTCGAAGATTG+CGG	0.600062	5.2:+23146757	MS.gene027718:CDS
CTGGATGGTACTTGTCGAAG+AGG	0.604675	5.2:-23146511	None:intergenic
GGAGGTCGTGGTAACGCCGG+AGG	0.608528	5.2:+23146470	MS.gene027718:CDS
AACTGAGTAACATCAATCAC+CGG	0.615568	5.2:-23146671	None:intergenic
ACGGTGGTGATGCATACCTC+CGG	0.616924	5.2:-23146486	None:intergenic
TTTGAAGGAGAACAAAGCGC+CGG	0.644488	5.2:+23146652	MS.gene027718:CDS
CTTTACCGAAGTAACCTGGA+TGG	0.684945	5.2:-23146526	None:intergenic
AAGAAGATTAAGGAGGCTGG+TGG	0.689384	5.2:+23146782	MS.gene027718:CDS
AAGAAGAACAGGAAGAAGCG+CGG	0.691929	5.2:+23146398	MS.gene027718:CDS
TGCGGAGAAGAAGATTAAGG+AGG	0.697175	5.2:+23146775	MS.gene027718:CDS
TCTTGTCGATGTTGACAATG+GGG	0.721275	5.2:-23146592	None:intergenic
AAACACCGCAAGCATCCCGG+AGG	0.740520	5.2:+23146452	MS.gene027718:CDS
ACTTGTCGAAGAGGATACGG+TGG	0.745852	5.2:-23146502	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTGGGTTTTTTAAGCTTTTA+GGG	+	chr5.2:23146693-23146712	MS.gene027718:CDS	25.0%
!!!	TTTGGGTTTTTTAAGCTTTT+AGG	+	chr5.2:23146692-23146711	MS.gene027718:CDS	25.0%
!!!	TTTTTTAAGCTTTTAGGGAA+AGG	+	chr5.2:23146698-23146717	MS.gene027718:CDS	25.0%
	TGATTGATGTTACTCAGTTT+GGG	+	chr5.2:23146675-23146694	MS.gene027718:CDS	30.0%
	TTTGATTCCACAAGATGTTA+AGG	+	chr5.2:23146622-23146641	MS.gene027718:CDS	30.0%
	AACTGAGTAACATCAATCAC+CGG	-	chr5.2:23146674-23146693	None:intergenic	35.0%
	ATCTTGTCGATGTTGACAAT+GGG	-	chr5.2:23146596-23146615	None:intergenic	35.0%
	GTGATTGATGTTACTCAGTT+TGG	+	chr5.2:23146674-23146693	MS.gene027718:CDS	35.0%
	TCTTCCTGTTCTTCTTGAAT+CGG	-	chr5.2:23146394-23146413	None:intergenic	35.0%
	AAGTACCATCCAGGTTACTT+CGG	+	chr5.2:23146521-23146540	MS.gene027718:CDS	40.0%
	AGCTTCGTCTTCACAACAAA+AGG	-	chr5.2:23146743-23146762	None:intergenic	40.0%
	CTTCCTGTTCTTCTTGAATC+GGG	-	chr5.2:23146393-23146412	None:intergenic	40.0%
	GATCTTGTCGATGTTGACAA+TGG	-	chr5.2:23146597-23146616	None:intergenic	40.0%
	GATTGCGGAGAAGAAGATTA+AGG	+	chr5.2:23146772-23146791	MS.gene027718:CDS	40.0%
	TCCACAAGATGTTAAGGAGA+AGG	+	chr5.2:23146628-23146647	MS.gene027718:CDS	40.0%
	TCTTGTCGATGTTGACAATG+GGG	-	chr5.2:23146595-23146614	None:intergenic	40.0%
!	GAAGCTGATTTCGAAGATTG+CGG	+	chr5.2:23146757-23146776	MS.gene027718:CDS	40.0%
!	TGTTAAGGAGAAGGCTTTGA+AGG	+	chr5.2:23146637-23146656	MS.gene027718:CDS	40.0%
!!	AAGGTGTTTTGCCGCAAAAT+CGG	+	chr5.2:23146717-23146736	MS.gene027718:CDS	40.0%
!!	ACAACAAAAGGCCGATTTTG+CGG	-	chr5.2:23146731-23146750	None:intergenic	40.0%
	AAGAAGAACAGGAAGAAGCG+CGG	+	chr5.2:23146398-23146417	MS.gene027718:CDS	45.0%
	AAGAAGATTAAGGAGGCTGG+TGG	+	chr5.2:23146782-23146801	MS.gene027718:CDS	45.0%
	CAACCCGATTCAAGAAGAAC+AGG	+	chr5.2:23146387-23146406	MS.gene027718:CDS	45.0%
	CCAACTTTACCGAAGTAACC+TGG	-	chr5.2:23146533-23146552	None:intergenic	45.0%
	CCAGGTTACTTCGGTAAAGT+TGG	+	chr5.2:23146530-23146549	MS.gene027718:CDS	45.0%
	CTTTACCGAAGTAACCTGGA+TGG	-	chr5.2:23146529-23146548	None:intergenic	45.0%
	GAGAAGAAGATTAAGGAGGC+TGG	+	chr5.2:23146779-23146798	MS.gene027718:CDS	45.0%
	GCCTTCTCCTTAACATCTTG+TGG	-	chr5.2:23146632-23146651	None:intergenic	45.0%
	GGTACTTGTCGAAGAGGATA+CGG	-	chr5.2:23146508-23146527	None:intergenic	45.0%
	TGCGGAGAAGAAGATTAAGG+AGG	+	chr5.2:23146775-23146794	MS.gene027718:CDS	45.0%
	TTTGAAGGAGAACAAAGCGC+CGG	+	chr5.2:23146652-23146671	MS.gene027718:CDS	45.0%
	ACTTGTCGAAGAGGATACGG+TGG	-	chr5.2:23146505-23146524	None:intergenic	50.0%
	CTCTTCGACAAGTACCATCC+AGG	+	chr5.2:23146512-23146531	MS.gene027718:CDS	50.0%
	CTGGATGGTACTTGTCGAAG+AGG	-	chr5.2:23146514-23146533	None:intergenic	50.0%
!	TTTTCCGATACGACCGTGTC+CGG	-	chr5.2:23146435-23146454	None:intergenic	50.0%
	GGAAAACACCGCAAGCATCC+CGG	+	chr5.2:23146449-23146468	MS.gene027718:CDS	55.0%
!	ACGGTGGTGATGCATACCTC+CGG	-	chr5.2:23146489-23146508	None:intergenic	55.0%
	AAACACCGCAAGCATCCCGG+AGG	+	chr5.2:23146452-23146471	MS.gene027718:CDS	60.0%
	CACGACCTCCGGGATGCTTG+CGG	-	chr5.2:23146460-23146479	None:intergenic	65.0%
	TCCGGCGTTACCACGACCTC+CGG	-	chr5.2:23146471-23146490	None:intergenic	65.0%
!	AGCGCCGGACACGGTCGTAT+CGG	+	chr5.2:23146428-23146447	MS.gene027718:CDS	65.0%
	CCGGCGTTACCACGACCTCC+GGG	-	chr5.2:23146470-23146489	None:intergenic	70.0%
	CGCAAGCATCCCGGAGGTCG+TGG	+	chr5.2:23146458-23146477	MS.gene027718:CDS	70.0%
!	CCCGGAGGTCGTGGTAACGC+CGG	+	chr5.2:23146467-23146486	MS.gene027718:CDS	70.0%
!	GGAGGTCGTGGTAACGCCGG+AGG	+	chr5.2:23146470-23146489	MS.gene027718:CDS	70.0%
	AAGCGCGGCCACGTCAGCGC+CGG	+	chr5.2:23146413-23146432	MS.gene027718:CDS	75.0%
!	GGCCACGTCAGCGCCGGACA+CGG	+	chr5.2:23146419-23146438	MS.gene027718:CDS	75.0%
!!	GACCGTGTCCGGCGCTGACG+TGG	-	chr5.2:23146424-23146443	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	23146383	23146826	23146383	ID=MS.gene027718
chr5.2	mRNA	23146383	23146826	23146383	ID=MS.gene027718.t1;Parent=MS.gene027718
chr5.2	exon	23146383	23146826	23146383	ID=MS.gene027718.t1.exon1;Parent=MS.gene027718.t1
chr5.2	CDS	23146383	23146826	23146383	ID=cds.MS.gene027718.t1;Parent=MS.gene027718.t1

Gene Sequence

>MS.gene027718

ATGACAACCCGATTCAAGAAGAACAGGAAGAAGCGCGGCCACGTCAGCGCCGGACACGGTCGTATCGGAAAACACCGCAAGCATCCCGGAGGTCGTGGTAACGCCGGAGGTATGCATCACCACCGTATCCTCTTCGACAAGTACCATCCAGGTTACTTCGGTAAAGTTGGTATGCGTTACTTTCACAAGCTTCGCAACAAATTCTACAACCCCATTGTCAACATCGACAAGATCGCTTCTTTGATTCCACAAGATGTTAAGGAGAAGGCTTTGAAGGAGAACAAAGCGCCGGTGATTGATGTTACTCAGTTTGGGTTTTTTAAGCTTTTAGGGAAAGGTGTTTTGCCGCAAAATCGGCCTTTTGTTGTGAAGACGAAGCTGATTTCGAAGATTGCGGAGAAGAAGATTAAGGAGGCTGGTGGTGCTGTTGTTCTTACTGCTTAG

Protein sequence

>MS.gene027718.t1

MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNRPFVVKTKLISKIAEKKIKEAGGAVVLTA