AlfalfaGEDB | MS.gene29174

M. sativa cultivar XinJiangDaYe / MS.gene29174

KEGG: K02900
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00828.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29174.t1	XP_003613127.2	100	147	0	0	1	147	1	147	9.30E-52	213

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29174.t1	P49637	77.6	147	32	1	1	147	1	146	6.1e-44	178.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29174.t1	G7JYX5	100.0	147	0	0	1	147	1	147	6.7e-52	213.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051904	MS.gene29174	0.80741	5.12E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene29174	MS.gene74837	PPI
MS.gene70668	MS.gene29174	PPI
MS.gene74837	MS.gene29174	PPI
MS.gene44297	MS.gene29174	PPI
MS.gene027718	MS.gene29174	PPI
MS.gene26005	MS.gene29174	PPI
MS.gene29174	MS.gene86214	PPI
MS.gene022341	MS.gene29174	PPI
MS.gene86214	MS.gene29174	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29174.t1	MTR_5g033090	100.000	147	0	0	1	147	1	147	2.98e-104	294
MS.gene29174.t1	MTR_3g077050	99.320	147	1	0	1	147	1	147	7.09e-104	293
MS.gene29174.t1	MTR_8g069460	84.459	148	22	1	1	147	1	148	8.14e-88	253
MS.gene29174.t1	MTR_4g119380	53.741	147	14	3	1	147	100	192	3.35e-36	124

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29174.t1	AT1G70600	85.714	147	20	1	1	147	1	146	9.47e-89	255
MS.gene29174.t1	AT1G23290	83.673	147	23	1	1	147	1	146	3.32e-86	248
MS.gene29174.t1	AT1G12960	50.000	146	27	2	1	146	1	100	1.75e-36	121

Find 45 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGATTGATGTTACTCAGTT+TGG	0.310495	5.3:+22249790	MS.gene29174:CDS
TCTTCCTGTTCTTCTTGAAT+CGG	0.319606	5.3:-22249507	None:intergenic
TTTGATTCCACAAGATGTTA+AGG	0.375048	5.3:+22249738	MS.gene29174:CDS
TGTTAAGGAGAAGGCTTTGA+AGG	0.376961	5.3:+22249753	MS.gene29174:CDS
TGATTGATGTTACTCAGTTT+GGG	0.380111	5.3:+22249791	MS.gene29174:CDS
GATTGCGGAGAAGAAGATTA+AGG	0.381172	5.3:+22249888	MS.gene29174:CDS
CTTCCTGTTCTTCTTGAATC+GGG	0.382353	5.3:-22249506	None:intergenic
ATCTTGTCGATGTTGACAAT+GGG	0.394209	5.3:-22249709	None:intergenic
CGCAAGCATCCCGGAGGTCG+TGG	0.449325	5.3:+22249574	MS.gene29174:CDS
GCCTTCTCCTTAACATCTTG+TGG	0.452270	5.3:-22249745	None:intergenic
TGCTGTTGTTCTTACTGCTT+AGG	0.456887	5.3:+22249921	MS.gene29174:CDS
TCCGGCATTACCACGACCTC+CGG	0.457326	5.3:-22249584	None:intergenic
AGCTTCGTCTTCACAACAAA+AGG	0.468189	5.3:-22249856	None:intergenic
TCCAGGTTACTTCGGTAAAG+TGG	0.475563	5.3:+22249645	MS.gene29174:CDS
CCCACTTTACCGAAGTAACC+TGG	0.480893	5.3:-22249646	None:intergenic
CCCGGAGGTCGTGGTAATGC+CGG	0.487276	5.3:+22249583	MS.gene29174:CDS
CAACCCGATTCAAGAAGAAC+AGG	0.488410	5.3:+22249503	MS.gene29174:CDS
TGGGGTTGTAGAATTTGTTA+CGG	0.498120	5.3:-22249690	None:intergenic
TCCACAAGATGTTAAGGAGA+AGG	0.532638	5.3:+22249744	MS.gene29174:CDS
GGTCACGTCAGCGCCGGACA+TGG	0.543314	5.3:+22249535	MS.gene29174:CDS
CTCTTCGACAAGTACCATCC+AGG	0.549117	5.3:+22249628	MS.gene29174:CDS
AGCGCCGGACATGGTCGTAT+CGG	0.555274	5.3:+22249544	MS.gene29174:CDS
CCGGCATTACCACGACCTCC+GGG	0.559074	5.3:-22249583	None:intergenic
GCGGTGGTGATGCATACCTC+CGG	0.562090	5.3:-22249602	None:intergenic
AGAATTTGTTACGGAGCTTG+TGG	0.563774	5.3:-22249681	None:intergenic
CACGACCTCCGGGATGCTTG+CGG	0.564581	5.3:-22249573	None:intergenic
AAGCGCGGTCACGTCAGCGC+CGG	0.569923	5.3:+22249529	MS.gene29174:CDS
GGTACTTGTCGAAGAGGATG+CGG	0.572674	5.3:-22249621	None:intergenic
GAGAAGAAGATTAAGGAGGC+TGG	0.580387	5.3:+22249895	MS.gene29174:CDS
GATCTTGTCGATGTTGACAA+TGG	0.583929	5.3:-22249710	None:intergenic
GGAAAACACCGCAAGCATCC+CGG	0.587466	5.3:+22249565	MS.gene29174:CDS
CCAGGTTACTTCGGTAAAGT+GGG	0.594593	5.3:+22249646	MS.gene29174:CDS
AAGTACCATCCAGGTTACTT+CGG	0.598039	5.3:+22249637	MS.gene29174:CDS
GAAGCTGATTTCGAAGATTG+CGG	0.600062	5.3:+22249873	MS.gene29174:CDS
CTGGATGGTACTTGTCGAAG+AGG	0.607823	5.3:-22249627	None:intergenic
AACTGAGTAACATCAATCAC+CGG	0.615568	5.3:-22249787	None:intergenic
TTTGAAGGAGAACAAAGCGC+CGG	0.644488	5.3:+22249768	MS.gene29174:CDS
GGAGGTCGTGGTAATGCCGG+AGG	0.650377	5.3:+22249586	MS.gene29174:CDS
CTTTACCGAAGTAACCTGGA+TGG	0.684945	5.3:-22249642	None:intergenic
AAGAAGATTAAGGAGGCTGG+TGG	0.689384	5.3:+22249898	MS.gene29174:CDS
TGCGGAGAAGAAGATTAAGG+AGG	0.697175	5.3:+22249891	MS.gene29174:CDS
ACTTGTCGAAGAGGATGCGG+TGG	0.709791	5.3:-22249618	None:intergenic
AAGAAGAACAGGAAGAAGCG+CGG	0.712524	5.3:+22249514	MS.gene29174:CDS
TCTTGTCGATGTTGACAATG+GGG	0.721275	5.3:-22249708	None:intergenic
AAACACCGCAAGCATCCCGG+AGG	0.740520	5.3:+22249568	MS.gene29174:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTGGGTTTTTTAAGCTTTTA+GGG	+	chr5.3:22249809-22249828	MS.gene29174:CDS	25.0%
!!!	TTTGGGTTTTTTAAGCTTTT+AGG	+	chr5.3:22249808-22249827	MS.gene29174:CDS	25.0%
!!!	TTTTTTAAGCTTTTAGGGAA+AGG	+	chr5.3:22249814-22249833	MS.gene29174:CDS	25.0%
	TGATTGATGTTACTCAGTTT+GGG	+	chr5.3:22249791-22249810	MS.gene29174:CDS	30.0%
	TTTGATTCCACAAGATGTTA+AGG	+	chr5.3:22249738-22249757	MS.gene29174:CDS	30.0%
	AACTGAGTAACATCAATCAC+CGG	-	chr5.3:22249790-22249809	None:intergenic	35.0%
	ATCTTGTCGATGTTGACAAT+GGG	-	chr5.3:22249712-22249731	None:intergenic	35.0%
	GTGATTGATGTTACTCAGTT+TGG	+	chr5.3:22249790-22249809	MS.gene29174:CDS	35.0%
	TCTTCCTGTTCTTCTTGAAT+CGG	-	chr5.3:22249510-22249529	None:intergenic	35.0%
!!	ACAACAAAAGGCTGATTTTG+CGG	-	chr5.3:22249847-22249866	None:intergenic	35.0%
!!	TGGGGTTGTAGAATTTGTTA+CGG	-	chr5.3:22249693-22249712	None:intergenic	35.0%
	AAGTACCATCCAGGTTACTT+CGG	+	chr5.3:22249637-22249656	MS.gene29174:CDS	40.0%
	AGCTTCGTCTTCACAACAAA+AGG	-	chr5.3:22249859-22249878	None:intergenic	40.0%
	CTTCCTGTTCTTCTTGAATC+GGG	-	chr5.3:22249509-22249528	None:intergenic	40.0%
	GATCTTGTCGATGTTGACAA+TGG	-	chr5.3:22249713-22249732	None:intergenic	40.0%
	GATTGCGGAGAAGAAGATTA+AGG	+	chr5.3:22249888-22249907	MS.gene29174:CDS	40.0%
	TCCACAAGATGTTAAGGAGA+AGG	+	chr5.3:22249744-22249763	MS.gene29174:CDS	40.0%
	TCTTGTCGATGTTGACAATG+GGG	-	chr5.3:22249711-22249730	None:intergenic	40.0%
!	GAAGCTGATTTCGAAGATTG+CGG	+	chr5.3:22249873-22249892	MS.gene29174:CDS	40.0%
!	TGTTAAGGAGAAGGCTTTGA+AGG	+	chr5.3:22249753-22249772	MS.gene29174:CDS	40.0%
!!	AGAATTTGTTACGGAGCTTG+TGG	-	chr5.3:22249684-22249703	None:intergenic	40.0%
	AAGAAGAACAGGAAGAAGCG+CGG	+	chr5.3:22249514-22249533	MS.gene29174:CDS	45.0%
	AAGAAGATTAAGGAGGCTGG+TGG	+	chr5.3:22249898-22249917	MS.gene29174:CDS	45.0%
	CAACCCGATTCAAGAAGAAC+AGG	+	chr5.3:22249503-22249522	MS.gene29174:CDS	45.0%
	CCAGGTTACTTCGGTAAAGT+GGG	+	chr5.3:22249646-22249665	MS.gene29174:CDS	45.0%
	CTTTACCGAAGTAACCTGGA+TGG	-	chr5.3:22249645-22249664	None:intergenic	45.0%
	GAGAAGAAGATTAAGGAGGC+TGG	+	chr5.3:22249895-22249914	MS.gene29174:CDS	45.0%
	GCCTTCTCCTTAACATCTTG+TGG	-	chr5.3:22249748-22249767	None:intergenic	45.0%
	TCCAGGTTACTTCGGTAAAG+TGG	+	chr5.3:22249645-22249664	MS.gene29174:CDS	45.0%
	TGCGGAGAAGAAGATTAAGG+AGG	+	chr5.3:22249891-22249910	MS.gene29174:CDS	45.0%
	TTTGAAGGAGAACAAAGCGC+CGG	+	chr5.3:22249768-22249787	MS.gene29174:CDS	45.0%
!	TTTTCCGATACGACCATGTC+CGG	-	chr5.3:22249551-22249570	None:intergenic	45.0%
	CCCACTTTACCGAAGTAACC+TGG	-	chr5.3:22249649-22249668	None:intergenic	50.0%
	CTCTTCGACAAGTACCATCC+AGG	+	chr5.3:22249628-22249647	MS.gene29174:CDS	50.0%
	CTGGATGGTACTTGTCGAAG+AGG	-	chr5.3:22249630-22249649	None:intergenic	50.0%
	GGTACTTGTCGAAGAGGATG+CGG	-	chr5.3:22249624-22249643	None:intergenic	50.0%
	ACTTGTCGAAGAGGATGCGG+TGG	-	chr5.3:22249621-22249640	None:intergenic	55.0%
	GGAAAACACCGCAAGCATCC+CGG	+	chr5.3:22249565-22249584	MS.gene29174:CDS	55.0%
	AAACACCGCAAGCATCCCGG+AGG	+	chr5.3:22249568-22249587	MS.gene29174:CDS	60.0%
	TCCGGCATTACCACGACCTC+CGG	-	chr5.3:22249587-22249606	None:intergenic	60.0%
!	AGCGCCGGACATGGTCGTAT+CGG	+	chr5.3:22249544-22249563	MS.gene29174:CDS	60.0%
!	GCGGTGGTGATGCATACCTC+CGG	-	chr5.3:22249605-22249624	None:intergenic	60.0%
	CACGACCTCCGGGATGCTTG+CGG	-	chr5.3:22249576-22249595	None:intergenic	65.0%
	CCGGCATTACCACGACCTCC+GGG	-	chr5.3:22249586-22249605	None:intergenic	65.0%
!	CCCGGAGGTCGTGGTAATGC+CGG	+	chr5.3:22249583-22249602	MS.gene29174:CDS	65.0%
!	GGAGGTCGTGGTAATGCCGG+AGG	+	chr5.3:22249586-22249605	MS.gene29174:CDS	65.0%
	AAGCGCGGTCACGTCAGCGC+CGG	+	chr5.3:22249529-22249548	MS.gene29174:CDS	70.0%
	CGCAAGCATCCCGGAGGTCG+TGG	+	chr5.3:22249574-22249593	MS.gene29174:CDS	70.0%
!	GGTCACGTCAGCGCCGGACA+TGG	+	chr5.3:22249535-22249554	MS.gene29174:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	22249499	22249942	22249499	ID=MS.gene29174
chr5.3	mRNA	22249499	22249942	22249499	ID=MS.gene29174.t1;Parent=MS.gene29174
chr5.3	exon	22249499	22249942	22249499	ID=MS.gene29174.t1.exon1;Parent=MS.gene29174.t1
chr5.3	CDS	22249499	22249942	22249499	ID=cds.MS.gene29174.t1;Parent=MS.gene29174.t1

Gene Sequence

>MS.gene29174

ATGACAACCCGATTCAAGAAGAACAGGAAGAAGCGCGGTCACGTCAGCGCCGGACATGGTCGTATCGGAAAACACCGCAAGCATCCCGGAGGTCGTGGTAATGCCGGAGGTATGCATCACCACCGCATCCTCTTCGACAAGTACCATCCAGGTTACTTCGGTAAAGTGGGTATGCGTTACTTCCACAAGCTCCGTAACAAATTCTACAACCCCATTGTCAACATCGACAAGATCGCTTCTTTGATTCCACAAGATGTTAAGGAGAAGGCTTTGAAGGAGAACAAAGCGCCGGTGATTGATGTTACTCAGTTTGGGTTTTTTAAGCTTTTAGGGAAAGGTGTTTTGCCGCAAAATCAGCCTTTTGTTGTGAAGACGAAGCTGATTTCGAAGATTGCGGAGAAGAAGATTAAGGAGGCTGGTGGTGCTGTTGTTCTTACTGCTTAG

Protein sequence

>MS.gene29174.t1

MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQPFVVKTKLISKIAEKKIKEAGGAVVLTA