AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74837


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74837.t1 MTR_5g033090 100.000 147 0 0 1 147 1 147 2.98e-104 294
MS.gene74837.t1 MTR_3g077050 99.320 147 1 0 1 147 1 147 7.09e-104 293
MS.gene74837.t1 MTR_8g069460 84.459 148 22 1 1 147 1 148 8.14e-88 253
MS.gene74837.t1 MTR_4g119380 53.741 147 14 3 1 147 100 192 3.35e-36 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74837.t1 AT1G70600 85.714 147 20 1 1 147 1 146 9.47e-89 255
MS.gene74837.t1 AT1G23290 83.673 147 23 1 1 147 1 146 3.32e-86 248
MS.gene74837.t1 AT1G12960 50.000 146 27 2 1 146 1 100 1.75e-36 121

Find 0 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TGATTGATGTTACTCAGTTT+GGG - 23879:208323-208342 MS.gene74837:CDS 30.0%
TTCTTTAAGCTTCTTGGAAA+AGG - 23879:208346-208365 MS.gene74837:CDS 30.0%
!! AGCTTAGTCTTAACAACAAA+AGG + 23879:208391-208410 None:intergenic 30.0%
AACTGAGTAACATCAATCAC+AGG + 23879:208322-208341 None:intergenic 35.0%
AAGTATCATCCAGGTTACTT+CGG - 23879:208169-208188 MS.gene74837:CDS 35.0%
ATCTTGTCGATGTTGACAAT+AGG + 23879:208244-208263 None:intergenic 35.0%
GTGATTGATGTTACTCAGTT+TGG - 23879:208322-208341 MS.gene74837:CDS 35.0%
TCTTGTCGATGTTGACAATA+GGG + 23879:208243-208262 None:intergenic 35.0%
! TTTGGGTTCTTTAAGCTTCT+TGG - 23879:208340-208359 MS.gene74837:CDS 35.0%
AAGAAGAACAGGAAGAAGAG+AGG - 23879:208046-208065 MS.gene74837:CDS 40.0%
GATACTTGTCGAAGAGGATA+CGG + 23879:208156-208175 None:intergenic 40.0%
GATTGCTGAGAAGAAGATCA+AGG - 23879:208420-208439 MS.gene74837:CDS 40.0%
TCTCATTCCACAAGATGTGA+AGG - 23879:208270-208289 MS.gene74837:CDS 40.0%
TCTTCCTGTTCTTCTTGAAG+CGG + 23879:208042-208061 None:intergenic 40.0%
! ACAACAAAAGGCTGATTCTG+AGG + 23879:208379-208398 None:intergenic 40.0%
! TGTGAAGGAGAAAGCTTTGA+AGG - 23879:208285-208304 MS.gene74837:CDS 40.0%
AAGAAGATCAAGGAAGCTGG+TGG - 23879:208430-208449 MS.gene74837:CDS 45.0%
CTCTTCGACAAGTATCATCC+AGG - 23879:208160-208179 MS.gene74837:CDS 45.0%
CTGGATGATACTTGTCGAAG+AGG + 23879:208162-208181 None:intergenic 45.0%
CTTCCTGTTCTTCTTGAAGC+GGG + 23879:208041-208060 None:intergenic 45.0%
GAGAAGAAGATCAAGGAAGC+TGG - 23879:208427-208446 MS.gene74837:CDS 45.0%
GCTTTCTCCTTCACATCTTG+TGG + 23879:208280-208299 None:intergenic 45.0%
TCATCCAGGTTACTTCGGTA+AGG - 23879:208174-208193 MS.gene74837:CDS 45.0%
CCAACCTTACCGAAGTAACC+TGG + 23879:208181-208200 None:intergenic 50.0%
CCAGGTTACTTCGGTAAGGT+TGG - 23879:208178-208197 MS.gene74837:CDS 50.0%
CTTTCCGATACGACCATGAC+CGG + 23879:208083-208102 None:intergenic 50.0%
TGTCGAAGAGGATACGGTGA+TGG + 23879:208150-208169 None:intergenic 50.0%
CGACCCGCTTCAAGAAGAAC+AGG - 23879:208035-208054 MS.gene74837:CDS 55.0%
! ACGGTGATGGTGCATTCCTC+CGG + 23879:208137-208156 None:intergenic 55.0%
!! GGAAAGCATCGCAAGCATCC+CGG - 23879:208097-208116 MS.gene74837:CDS 55.0%
AAGAGAGGTCACGTGAGCGC+CGG - 23879:208061-208080 MS.gene74837:CDS 60.0%
! AAGCATCGCAAGCATCCCGG+AGG - 23879:208100-208119 MS.gene74837:CDS 60.0%
!! AGCGCCGGTCATGGTCGTAT+CGG - 23879:208076-208095 MS.gene74837:CDS 60.0%
TCCGGCGTTACCACGACCTC+CGG + 23879:208119-208138 None:intergenic 65.0%
CCGGCGTTACCACGACCTCC+GGG + 23879:208118-208137 None:intergenic 70.0%
CGCAAGCATCCCGGAGGTCG+TGG - 23879:208106-208125 MS.gene74837:CDS 70.0%
! CCCGGAGGTCGTGGTAACGC+CGG - 23879:208115-208134 MS.gene74837:CDS 70.0%
! GGAGGTCGTGGTAACGCCGG+AGG - 23879:208118-208137 MS.gene74837:CDS 70.0%
!! GGTCACGTGAGCGCCGGTCA+TGG - 23879:208067-208086 MS.gene74837:CDS 70.0%


Chromosome Type Strat End Strand Name
23879 gene 208031 208474 208031 ID=MS.gene74837
23879 mRNA 208031 208474 208031 ID=MS.gene74837.t1;Parent=MS.gene74837
23879 exon 208031 208474 208031 ID=MS.gene74837.t1.exon1;Parent=MS.gene74837.t1
23879 CDS 208031 208474 208031 ID=cds.MS.gene74837.t1;Parent=MS.gene74837.t1
Gene Sequence

>MS.gene74837

ATGACGACCCGCTTCAAGAAGAACAGGAAGAAGAGAGGTCACGTGAGCGCCGGTCATGGTCGTATCGGAAAGCATCGCAAGCATCCCGGAGGTCGTGGTAACGCCGGAGGAATGCACCATCACCGTATCCTCTTCGACAAGTATCATCCAGGTTACTTCGGTAAGGTTGGTATGCGTTACTTTCACAAGCTTCGTAACAAATTCTATAACCCTATTGTCAACATCGACAAGATCGCTTCTCTCATTCCACAAGATGTGAAGGAGAAAGCTTTGAAGGAGAATAAAGCTCCTGTGATTGATGTTACTCAGTTTGGGTTCTTTAAGCTTCTTGGAAAAGGTGTTTTGCCTCAGAATCAGCCTTTTGTTGTTAAGACTAAGCTGATTTCGAAGATTGCTGAGAAGAAGATCAAGGAAGCTGGTGGTGCTGTTGTTCTTACTGCTTGA

Protein sequence

>MS.gene74837.t1

MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQPFVVKTKLISKIAEKKIKEAGGAVVLTA