Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023766.t1 | RHN56340.1 | 88 | 158 | 19 | 0 | 1 | 158 | 1 | 158 | 9.60E-72 | 279.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023766.t1 | A0A396HSM2 | 88.0 | 158 | 19 | 0 | 1 | 158 | 1 | 158 | 6.9e-72 | 279.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene015160 | MS.gene023766 | PPI |
| MS.gene010343 | MS.gene023766 | PPI |
| MS.gene93488 | MS.gene023766 | PPI |
| MS.gene94537 | MS.gene023766 | PPI |
| MS.gene93489 | MS.gene023766 | PPI |
| MS.gene017970 | MS.gene023766 | PPI |
| MS.gene79416 | MS.gene023766 | PPI |
| MS.gene94530 | MS.gene023766 | PPI |
| MS.gene055988 | MS.gene023766 | PPI |
| MS.gene94534 | MS.gene023766 | PPI |
| MS.gene94527 | MS.gene023766 | PPI |
| MS.gene28215 | MS.gene023766 | PPI |
| MS.gene94533 | MS.gene023766 | PPI |
| MS.gene94532 | MS.gene023766 | PPI |
| MS.gene041075 | MS.gene023766 | PPI |
| MS.gene97792 | MS.gene023766 | PPI |
| MS.gene047623 | MS.gene023766 | PPI |
| MS.gene28214 | MS.gene023766 | PPI |
| MS.gene28213 | MS.gene023766 | PPI |
| MS.gene79417 | MS.gene023766 | PPI |
| MS.gene93487 | MS.gene023766 | PPI |
| MS.gene017995 | MS.gene023766 | PPI |
| MS.gene52667 | MS.gene023766 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023766.t1 | MTR_5g068000 | 88.889 | 153 | 17 | 0 | 2 | 154 | 1 | 153 | 6.87e-92 | 268 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCTCCAATCAACCTTTA+AGG | 0.240288 | 5.4:-53159431 | MS.gene023766:CDS |
| AAATTTACCTGCGACCTTAA+AGG | 0.244607 | 5.4:+53159417 | None:intergenic |
| GAACAACAAGTAACAATTAA+TGG | 0.277286 | 5.4:-53159778 | MS.gene023766:CDS |
| TCCATTGTTTCTCTCACTTT+AGG | 0.294080 | 5.4:+53159721 | None:intergenic |
| TTTGACGTTCCCAAGCAATT+TGG | 0.318565 | 5.4:+53159451 | None:intergenic |
| CATCTTCCACAACCGATTAA+TGG | 0.339547 | 5.4:-53159170 | MS.gene023766:CDS |
| GAGAGAAACAATGGAGTATA+TGG | 0.346210 | 5.4:-53159713 | MS.gene023766:intron |
| CTGCGACCTTAAAGGTTGAT+TGG | 0.360105 | 5.4:+53159425 | None:intergenic |
| CTTCAAGCAATTTCAAAAGT+AGG | 0.364624 | 5.4:-53159948 | MS.gene023766:CDS |
| GTGACGAAGACGTTCTCTTT+CGG | 0.384876 | 5.4:+53159849 | None:intergenic |
| GGTTGGTTTGGACCATTAAT+CGG | 0.394654 | 5.4:+53159158 | None:intergenic |
| GTTTCTCTCACTTTAGGATC+AGG | 0.407404 | 5.4:+53159727 | None:intergenic |
| TGGGGTAACACAGGTTGGTT+TGG | 0.407758 | 5.4:+53159146 | None:intergenic |
| ATAATTTAATTGTTGAAGAT+GGG | 0.408228 | 5.4:+53159127 | None:intergenic |
| GATAATTTAATTGTTGAAGA+TGG | 0.408311 | 5.4:+53159126 | None:intergenic |
| CATTATAGGTCCAAATTGCT+TGG | 0.413099 | 5.4:-53159461 | MS.gene023766:intron |
| TTCAAGCAATTTCAAAAGTA+GGG | 0.416269 | 5.4:-53159947 | MS.gene023766:CDS |
| TGTTCAACAATCTGACCAAC+TGG | 0.418248 | 5.4:+53159796 | None:intergenic |
| AAAAGCTCTGGGTACATAGC+TGG | 0.446339 | 5.4:+53159919 | None:intergenic |
| GGCTGCAGAAACTTAACAAC+CGG | 0.456089 | 5.4:-53158970 | MS.gene023766:CDS |
| CATTAATCGGTTGTGGAAGA+TGG | 0.472050 | 5.4:+53159171 | None:intergenic |
| CTTCTACTTTGCGTATCCAC+CGG | 0.472608 | 5.4:+53158951 | None:intergenic |
| ATTATAGGTCCAAATTGCTT+GGG | 0.503389 | 5.4:-53159460 | MS.gene023766:intron |
| TTTGGACCATTAATCGGTTG+TGG | 0.503702 | 5.4:+53159164 | None:intergenic |
| TCACGTAGATATTGAAAGAC+TGG | 0.509822 | 5.4:+53159886 | None:intergenic |
| CAATCAACCTTTAAGGTCGC+AGG | 0.515350 | 5.4:-53159424 | MS.gene023766:intron |
| AAGAACATTATGATGTCTCT+AGG | 0.527328 | 5.4:-53159991 | None:intergenic |
| GAAGATGGGGTAACACAGGT+TGG | 0.553132 | 5.4:+53159141 | None:intergenic |
| GAAATTACTAAGATTCCAGT+TGG | 0.554578 | 5.4:-53159811 | MS.gene023766:CDS |
| TAATTTAATTGTTGAAGATG+GGG | 0.559952 | 5.4:+53159128 | None:intergenic |
| TGTTGAAGATGGGGTAACAC+AGG | 0.592099 | 5.4:+53159137 | None:intergenic |
| TCCTAAAGTGAGAGAAACAA+TGG | 0.600914 | 5.4:-53159722 | MS.gene023766:CDS |
| ATGTCTCTAGGTCGAACTGA+AGG | 0.617564 | 5.4:-53159979 | MS.gene023766:CDS |
| GCTCTGGGTACATAGCTGGA+AGG | 0.644070 | 5.4:+53159923 | None:intergenic |
| AATAACAATATATACAGCCA+CGG | 0.647803 | 5.4:-53158994 | MS.gene023766:CDS |
| TGCAGAAACTTAACAACCGG+TGG | 0.674360 | 5.4:-53158967 | MS.gene023766:CDS |
| AAATTATCATGCTCAAAACG+AGG | 0.697419 | 5.4:-53159111 | MS.gene023766:intron |
| TTAAGTTTCTGCAGCCACCG+TGG | 0.730006 | 5.4:+53158977 | None:intergenic |
| AACAATATATACAGCCACGG+TGG | 0.744149 | 5.4:-53158991 | MS.gene023766:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATTGATTTAATATACAT+AGG | - | chr5.4:53159585-53159604 | MS.gene023766:intron | 10.0% |
| !! | ATAATTTAATTGTTGAAGAT+GGG | + | chr5.4:53159812-53159831 | None:intergenic | 15.0% |
| !! | ATATGTTACATTTACTAATT+AGG | - | chr5.4:53159385-53159404 | MS.gene023766:intron | 15.0% |
| !! | TATGTTACATTTACTAATTA+GGG | - | chr5.4:53159386-53159405 | MS.gene023766:intron | 15.0% |
| !! | TTATATTGCAACTATTAATT+AGG | + | chr5.4:53159351-53159370 | None:intergenic | 15.0% |
| !! | TTATTCTTCAATTAAAATCA+AGG | + | chr5.4:53159927-53159946 | None:intergenic | 15.0% |
| !! | GATAATTTAATTGTTGAAGA+TGG | + | chr5.4:53159813-53159832 | None:intergenic | 20.0% |
| !! | TAATTTAATTGTTGAAGATG+GGG | + | chr5.4:53159811-53159830 | None:intergenic | 20.0% |
| !! | TCTAATCAATTATTTGGTAT+TGG | - | chr5.4:53159688-53159707 | MS.gene023766:intron | 20.0% |
| !!! | AATCTTCTAAAGTAAATTCA+TGG | - | chr5.4:53159270-53159289 | MS.gene023766:intron | 20.0% |
| ! | AATAACAATATATACAGCCA+CGG | - | chr5.4:53159942-53159961 | MS.gene023766:CDS | 25.0% |
| ! | ATAGTTGCAATATAAGAGAA+AGG | - | chr5.4:53159356-53159375 | MS.gene023766:intron | 25.0% |
| ! | GAACAACAAGTAACAATTAA+TGG | - | chr5.4:53159158-53159177 | MS.gene023766:CDS | 25.0% |
| ! | GTGTGTATTTATTTGAATCT+TGG | - | chr5.4:53159305-53159324 | MS.gene023766:intron | 25.0% |
| ! | TCAACGTCTAATCAATTATT+TGG | - | chr5.4:53159682-53159701 | MS.gene023766:intron | 25.0% |
| ! | TTCAAGCAATTTCAAAAGTA+GGG | - | chr5.4:53158989-53159008 | MS.gene023766:CDS | 25.0% |
| !! | AAAGTTTCACTCTTGTTTAA+TGG | - | chr5.4:53159418-53159437 | MS.gene023766:intron | 25.0% |
| !! | AAGTTTCACTCTTGTTTAAT+GGG | - | chr5.4:53159419-53159438 | MS.gene023766:intron | 25.0% |
| !!! | AAATTCATGGAATTTGTTGT+TGG | - | chr5.4:53159283-53159302 | MS.gene023766:intron | 25.0% |
| AAATTATCATGCTCAAAACG+AGG | - | chr5.4:53159825-53159844 | MS.gene023766:CDS | 30.0% | |
| ATCTACGTGAGACTAAAAAA+AGG | - | chr5.4:53159063-53159082 | MS.gene023766:intron | 30.0% | |
| ATTATAGGTCCAAATTGCTT+GGG | - | chr5.4:53159476-53159495 | MS.gene023766:intron | 30.0% | |
| CTTCAAGCAATTTCAAAAGT+AGG | - | chr5.4:53158988-53159007 | MS.gene023766:CDS | 30.0% | |
| GAAATTACTAAGATTCCAGT+TGG | - | chr5.4:53159125-53159144 | MS.gene023766:CDS | 30.0% | |
| TCTACGTGAGACTAAAAAAA+GGG | - | chr5.4:53159064-53159083 | MS.gene023766:intron | 30.0% | |
| AAAGCTCCAATCAACCTTTA+AGG | - | chr5.4:53159505-53159524 | MS.gene023766:intron | 35.0% | |
| AAATTTACCTGCGACCTTAA+AGG | + | chr5.4:53159522-53159541 | None:intergenic | 35.0% | |
| CATTATAGGTCCAAATTGCT+TGG | - | chr5.4:53159475-53159494 | MS.gene023766:intron | 35.0% | |
| GACTAGAGAAAACTACGATT+TGG | + | chr5.4:53159661-53159680 | None:intergenic | 35.0% | |
| GAGAGAAACAATGGAGTATA+TGG | - | chr5.4:53159223-53159242 | MS.gene023766:intron | 35.0% | |
| GATCATCTTCAAAAAGCTCT+GGG | + | chr5.4:53159031-53159050 | None:intergenic | 35.0% | |
| TCACGTAGATATTGAAAGAC+TGG | + | chr5.4:53159053-53159072 | None:intergenic | 35.0% | |
| TCCTAAAGTGAGAGAAACAA+TGG | - | chr5.4:53159214-53159233 | MS.gene023766:intron | 35.0% | |
| ! | TCCATTGTTTCTCTCACTTT+AGG | + | chr5.4:53159218-53159237 | None:intergenic | 35.0% |
| !!! | CACTAGTTTTCGTGCATTAT+AGG | - | chr5.4:53159461-53159480 | MS.gene023766:intron | 35.0% |
| AACAATATATACAGCCACGG+TGG | - | chr5.4:53159945-53159964 | MS.gene023766:CDS | 40.0% | |
| CATCTTCCACAACCGATTAA+TGG | - | chr5.4:53159766-53159785 | MS.gene023766:CDS | 40.0% | |
| CATTAATCGGTTGTGGAAGA+TGG | + | chr5.4:53159768-53159787 | None:intergenic | 40.0% | |
| GGATCATCTTCAAAAAGCTC+TGG | + | chr5.4:53159032-53159051 | None:intergenic | 40.0% | |
| TCTGATCCTGAAAAACTCGT+CGG | + | chr5.4:53159714-53159733 | None:intergenic | 40.0% | |
| TGTTCAACAATCTGACCAAC+TGG | + | chr5.4:53159143-53159162 | None:intergenic | 40.0% | |
| TTTGACGTTCCCAAGCAATT+TGG | + | chr5.4:53159488-53159507 | None:intergenic | 40.0% | |
| TTTGGACCATTAATCGGTTG+TGG | + | chr5.4:53159775-53159794 | None:intergenic | 40.0% | |
| !! | GGTTGGTTTGGACCATTAAT+CGG | + | chr5.4:53159781-53159800 | None:intergenic | 40.0% |
| !! | GTTTCTCTCACTTTAGGATC+AGG | + | chr5.4:53159212-53159231 | None:intergenic | 40.0% |
| !!! | ACGAGTTTTTCAGGATCAGA+CGG | - | chr5.4:53159714-53159733 | MS.gene023766:CDS | 40.0% |
| ATGTCTCTAGGTCGAACTGA+AGG | - | chr5.4:53158957-53158976 | MS.gene023766:CDS | 45.0% | |
| GGCTGCAGAAACTTAACAAC+CGG | - | chr5.4:53159966-53159985 | MS.gene023766:CDS | 45.0% | |
| GTGACGAAGACGTTCTCTTT+CGG | + | chr5.4:53159090-53159109 | None:intergenic | 45.0% | |
| TGCAGAAACTTAACAACCGG+TGG | - | chr5.4:53159969-53159988 | MS.gene023766:CDS | 45.0% | |
| TGTTGAAGATGGGGTAACAC+AGG | + | chr5.4:53159802-53159821 | None:intergenic | 45.0% | |
| ! | CTGCGACCTTAAAGGTTGAT+TGG | + | chr5.4:53159514-53159533 | None:intergenic | 45.0% |
| ! | TATTGGCCGACGAGTTTTTC+AGG | - | chr5.4:53159705-53159724 | MS.gene023766:intron | 45.0% |
| !! | AAAAGCTCTGGGTACATAGC+TGG | + | chr5.4:53159020-53159039 | None:intergenic | 45.0% |
| !! | CAATCAACCTTTAAGGTCGC+AGG | - | chr5.4:53159512-53159531 | MS.gene023766:intron | 45.0% |
| GAAGATGGGGTAACACAGGT+TGG | + | chr5.4:53159798-53159817 | None:intergenic | 50.0% | |
| TTAAGTTTCTGCAGCCACCG+TGG | + | chr5.4:53159962-53159981 | None:intergenic | 50.0% | |
| !! | TGGGGTAACACAGGTTGGTT+TGG | + | chr5.4:53159793-53159812 | None:intergenic | 50.0% |
| ! | GCTCTGGGTACATAGCTGGA+AGG | + | chr5.4:53159016-53159035 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 53158954 | 53160004 | 53158954 | ID=MS.gene023766 |
| chr5.4 | mRNA | 53158954 | 53160004 | 53158954 | ID=MS.gene023766.t1;Parent=MS.gene023766 |
| chr5.4 | exon | 53159714 | 53160004 | 53159714 | ID=MS.gene023766.t1.exon1;Parent=MS.gene023766.t1 |
| chr5.4 | CDS | 53159714 | 53160004 | 53159714 | ID=cds.MS.gene023766.t1;Parent=MS.gene023766.t1 |
| chr5.4 | exon | 53159425 | 53159475 | 53159425 | ID=MS.gene023766.t1.exon2;Parent=MS.gene023766.t1 |
| chr5.4 | CDS | 53159425 | 53159475 | 53159425 | ID=cds.MS.gene023766.t1;Parent=MS.gene023766.t1 |
| chr5.4 | exon | 53159112 | 53159231 | 53159112 | ID=MS.gene023766.t1.exon3;Parent=MS.gene023766.t1 |
| chr5.4 | CDS | 53159112 | 53159231 | 53159112 | ID=cds.MS.gene023766.t1;Parent=MS.gene023766.t1 |
| chr5.4 | exon | 53158954 | 53159016 | 53158954 | ID=MS.gene023766.t1.exon4;Parent=MS.gene023766.t1 |
| chr5.4 | CDS | 53158954 | 53159016 | 53158954 | ID=cds.MS.gene023766.t1;Parent=MS.gene023766.t1 |
| Gene Sequence |
| Protein sequence |
