AlfalfaGEDB | MS.gene94527

M. sativa cultivar XinJiangDaYe / MS.gene94527

KEGG: -
GO: -
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94527.t1	AES69209.1	82.9	140	24	0	3	142	2	141	1.40E-57	232.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94527.t1	Q5M974	41.9	62	36	0	79	140	63	124	5.1e-11	68.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94527.t1	G7IWF8	82.9	140	24	0	3	142	2	141	1.0e-57	232.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene94527	MS.gene041075	PPI
MS.gene94527	MS.gene28214	PPI
MS.gene94527	MS.gene010008	PPI
MS.gene94527	MS.gene051416	PPI
MS.gene94527	MS.gene93488	PPI
MS.gene28215	MS.gene94527	PPI
MS.gene94532	MS.gene94527	PPI
MS.gene065402	MS.gene94527	PPI
MS.gene43652	MS.gene94527	PPI
MS.gene61920	MS.gene94527	PPI
MS.gene79416	MS.gene94527	PPI
MS.gene97792	MS.gene94527	PPI
MS.gene94527	MS.gene017970	PPI
MS.gene047623	MS.gene94527	PPI
MS.gene94527	MS.gene055988	PPI
MS.gene61922	MS.gene94527	PPI
MS.gene94527	MS.gene94530	PPI
MS.gene43658	MS.gene94527	PPI
MS.gene94527	MS.gene79417	PPI
MS.gene93487	MS.gene94527	PPI
MS.gene28213	MS.gene94527	PPI
MS.gene94527	MS.gene61924	PPI
MS.gene47429	MS.gene94527	PPI
MS.gene017995	MS.gene94527	PPI
MS.gene94527	MS.gene023766	PPI
MS.gene010343	MS.gene94527	PPI
MS.gene029745	MS.gene94527	PPI
MS.gene94527	MS.gene010006	PPI
MS.gene94534	MS.gene94527	PPI
MS.gene79417	MS.gene94527	PPI
MS.gene61921	MS.gene94527	PPI
MS.gene94527	MS.gene94537	PPI
MS.gene065401	MS.gene94527	PPI
MS.gene94527	MS.gene023764	PPI
MS.gene94527	MS.gene94533	PPI
MS.gene94527	MS.gene52667	PPI
MS.gene93489	MS.gene94527	PPI
MS.gene015160	MS.gene94527	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94527.t1	MTR_3g025380	82.857	140	24	0	3	142	2	141	2.06e-73	216
MS.gene94527.t1	MTR_3g025510	46.980	149	69	3	1	139	1	149	3.91e-38	127
MS.gene94527.t1	MTR_3g025550	84.746	59	9	0	84	142	6	64	6.74e-34	113
MS.gene94527.t1	MTR_3g025620	61.111	54	21	0	89	142	2	55	9.51e-20	77.4
MS.gene94527.t1	MTR_3g088080	37.209	86	47	2	56	141	6	84	2.15e-15	67.0
MS.gene94527.t1	MTR_5g041310	36.471	85	50	2	57	140	79	160	1.69e-14	67.8
MS.gene94527.t1	MTR_7g074170	46.429	56	30	0	85	140	335	390	3.57e-14	68.6
MS.gene94527.t1	MTR_2g058360	47.619	63	29	2	79	139	80	140	3.63e-14	65.5
MS.gene94527.t1	MTR_5g041330	35.294	85	51	2	57	140	69	150	2.36e-13	64.7
MS.gene94527.t1	MTR_3g072630	31.765	85	57	1	57	140	182	266	6.40e-13	64.7
MS.gene94527.t1	MTR_3g072630	31.765	85	57	1	57	140	182	266	7.93e-13	64.3
MS.gene94527.t1	MTR_1g114620	36.000	75	46	1	66	140	237	309	5.58e-12	62.0
MS.gene94527.t1	MTR_8g056930	40.000	75	41	2	68	139	90	163	6.04e-12	60.1
MS.gene94527.t1	MTR_0186s0090	40.000	75	41	2	68	139	90	163	9.19e-12	59.7
MS.gene94527.t1	MTR_6g079660	34.211	76	49	1	66	140	205	280	1.00e-11	61.6
MS.gene94527.t1	MTR_4g110100	40.984	61	36	0	80	140	345	405	1.32e-11	61.2
MS.gene94527.t1	MTR_2g097620	30.159	63	44	0	79	141	173	235	1.70e-11	60.8
MS.gene94527.t1	MTR_7g092960	43.333	60	32	1	79	136	37	96	1.76e-11	57.4
MS.gene94527.t1	MTR_5g088720	32.000	75	51	0	66	140	217	291	2.07e-11	60.5
MS.gene94527.t1	MTR_1g051105	43.478	46	26	0	95	140	159	204	2.60e-11	59.7
MS.gene94527.t1	MTR_5g088720	32.000	75	51	0	66	140	171	245	2.66e-11	60.1
MS.gene94527.t1	MTR_5g088720	32.000	75	51	0	66	140	176	250	2.72e-11	60.1
MS.gene94527.t1	MTR_6g016350	43.750	48	27	0	93	140	309	356	4.81e-11	59.3
MS.gene94527.t1	MTR_5g058530	52.381	42	20	0	95	136	224	265	4.98e-11	59.3
MS.gene94527.t1	MTR_2g007340	41.379	58	34	0	79	136	185	242	5.27e-11	59.3
MS.gene94527.t1	MTR_1g114320	39.216	51	31	0	90	140	189	239	5.54e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94527.t1	AT5G60820	45.283	53	29	0	89	141	366	418	1.14e-13	67.0
MS.gene94527.t1	AT3G02340	53.191	47	22	0	94	140	334	380	1.63e-13	66.6
MS.gene94527.t1	AT3G02340	53.191	47	22	0	94	140	334	380	1.63e-13	66.6
MS.gene94527.t1	AT3G60080	39.286	56	34	0	85	140	159	214	1.70e-13	66.2
MS.gene94527.t1	AT1G60360	47.826	46	24	0	95	140	224	269	1.81e-13	66.2
MS.gene94527.t1	AT1G14200	38.710	62	38	0	79	140	93	154	3.24e-13	63.9
MS.gene94527.t1	AT5G08139	41.818	55	32	0	86	140	300	354	3.29e-13	65.9
MS.gene94527.t1	AT4G12210	47.619	63	28	2	79	136	135	197	3.98e-13	63.9
MS.gene94527.t1	AT3G10815	37.097	62	39	0	79	140	105	166	8.52e-13	63.2
MS.gene94527.t1	AT2G44330	52.174	46	22	0	95	140	96	141	1.31e-12	62.0
MS.gene94527.t1	AT5G15820	47.059	51	27	0	90	140	286	336	1.46e-12	63.9
MS.gene94527.t1	AT1G18770	33.673	98	53	2	55	140	7	104	2.58e-12	59.7
MS.gene94527.t1	AT1G21960	37.500	88	50	2	58	140	116	203	3.27e-12	61.6
MS.gene94527.t1	AT4G12190	46.032	63	29	2	79	136	4	66	4.19e-12	58.2
MS.gene94527.t1	AT4G05350	36.170	94	49	4	54	136	108	201	4.49e-12	61.2
MS.gene94527.t1	AT2G40830	31.818	88	57	2	57	141	149	236	6.27e-12	62.0
MS.gene94527.t1	AT2G40830	31.818	88	57	2	57	141	149	236	6.27e-12	62.0
MS.gene94527.t1	AT2G40830	31.818	88	57	2	57	141	149	236	6.27e-12	62.0
MS.gene94527.t1	AT2G40830	31.818	88	57	2	57	141	149	236	6.27e-12	62.0
MS.gene94527.t1	AT3G30460	44.444	63	33	1	81	141	83	145	1.01e-11	59.3
MS.gene94527.t1	AT1G55530	29.730	74	47	1	67	140	200	268	1.12e-11	61.2
MS.gene94527.t1	AT1G55530	29.730	74	47	1	67	140	200	268	1.12e-11	61.2
MS.gene94527.t1	AT3G30460	44.068	59	33	0	81	139	119	177	1.27e-11	59.7
MS.gene94527.t1	AT1G55530	29.730	74	47	1	67	140	167	235	1.30e-11	60.8
MS.gene94527.t1	AT5G54990	48.889	45	23	0	92	136	171	215	1.74e-11	60.1
MS.gene94527.t1	AT3G13430	31.081	74	43	1	75	140	197	270	2.03e-11	60.5
MS.gene94527.t1	AT3G13430	31.081	74	43	1	75	140	197	270	2.03e-11	60.5
MS.gene94527.t1	AT3G13430	31.081	74	43	1	75	140	197	270	2.03e-11	60.5
MS.gene94527.t1	AT3G13430	31.081	74	43	1	75	140	197	270	2.03e-11	60.5
MS.gene94527.t1	AT1G18780	46.296	54	29	0	87	140	269	322	2.12e-11	60.5
MS.gene94527.t1	AT5G64920	36.207	58	37	0	79	136	241	298	2.69e-11	60.1
MS.gene94527.t1	AT4G12140	41.538	65	34	2	79	139	135	199	3.53e-11	58.9
MS.gene94527.t1	AT2G29840	47.059	51	27	0	90	140	239	289	4.00e-11	59.7
MS.gene94527.t1	AT1G18760	45.652	46	25	0	95	140	175	220	6.83e-11	58.2
MS.gene94527.t1	AT3G56580	26.241	141	91	5	3	140	101	231	7.10e-11	58.9
MS.gene94527.t1	AT3G56580	26.241	141	91	5	3	140	101	231	7.10e-11	58.9
MS.gene94527.t1	AT3G56580	26.241	141	91	5	3	140	101	231	7.10e-11	58.9
MS.gene94527.t1	AT1G72310	50.000	50	24	1	91	139	123	172	8.73e-11	58.5

Find 22 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTTGTTTGGCGGAGCTTT+TGG	0.249403	3.2:+15402546	MS.gene94527:CDS
TATGATGTGTTCCATTTGTT+TGG	0.364328	3.2:+15402534	MS.gene94527:CDS
GAATCTCGTTGATAAGGTTA+TGG	0.379677	3.2:-15402420	None:intergenic
CATAAACTAAGTCACATATT+AGG	0.380528	3.2:-15402676	None:intergenic
CAAAAGCTCCGCCAAACAAA+TGG	0.424226	3.2:-15402545	None:intergenic
ACGAAGAATGTATCATGAAA+TGG	0.429864	3.2:+15402611	MS.gene94527:CDS
TAGCTTGAATCTCGTTGATA+AGG	0.444704	3.2:-15402426	None:intergenic
GAATGTATCATGAAATGGTT+TGG	0.470491	3.2:+15402616	MS.gene94527:CDS
ATTTCATGATACATTCTTCG+TGG	0.485172	3.2:-15402609	None:intergenic
AATTAGAGTTGAAGAAGGAA+AGG	0.503387	3.2:+15402501	MS.gene94527:CDS
GATACCGATCAGAATTTCGT+TGG	0.508405	3.2:-15402471	None:intergenic
GATGTGTTCCATTTGTTTGG+CGG	0.523172	3.2:+15402537	MS.gene94527:CDS
GAACAATTTGACGACACAAA+GGG	0.529813	3.2:-15402654	None:intergenic
TCGTGGTACAAATGCGAACA+CGG	0.571244	3.2:-15402592	None:intergenic
GCTCGTCGTCGTCATACTCA+TGG	0.572555	3.2:+15402346	MS.gene94527:CDS
TTATGGTGGCGAACAACAGA+AGG	0.604056	3.2:-15402403	None:intergenic
CTATCCAACGAAATTCTGAT+CGG	0.613882	3.2:+15402467	MS.gene94527:CDS
TGGTTGGAACGCAAGCAACT+CGG	0.615833	3.2:+15402566	MS.gene94527:CDS
GGAACAATTTGACGACACAA+AGG	0.620591	3.2:-15402655	None:intergenic
TCTCGTTGATAAGGTTATGG+TGG	0.632126	3.2:-15402417	None:intergenic
ATAGCACTTCTTATATTACG+TGG	0.670123	3.2:-15402271	None:intergenic
ATGGTACAAATCGATCGACA+AGG	0.741500	3.2:+15402365	MS.gene94527:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCAAAAATTAGAGTTGAAGA+AGG	+	chr3.2:15402496-15402515	MS.gene94527:CDS	25.0%
	AATTAGAGTTGAAGAAGGAA+AGG	+	chr3.2:15402501-15402520	MS.gene94527:CDS	30.0%
	ACGAAGAATGTATCATGAAA+TGG	+	chr3.2:15402611-15402630	MS.gene94527:CDS	30.0%
	ATAGCACTTCTTATATTACG+TGG	-	chr3.2:15402274-15402293	None:intergenic	30.0%
	ATTTCATGATACATTCTTCG+TGG	-	chr3.2:15402612-15402631	None:intergenic	30.0%
	GAATGTATCATGAAATGGTT+TGG	+	chr3.2:15402616-15402635	MS.gene94527:CDS	30.0%
	TATGATGTGTTCCATTTGTT+TGG	+	chr3.2:15402534-15402553	MS.gene94527:CDS	30.0%
	CTATCCAACGAAATTCTGAT+CGG	+	chr3.2:15402467-15402486	MS.gene94527:CDS	35.0%
	GAACAATTTGACGACACAAA+GGG	-	chr3.2:15402657-15402676	None:intergenic	35.0%
	TAGCTTGAATCTCGTTGATA+AGG	-	chr3.2:15402429-15402448	None:intergenic	35.0%
!	GAATCTCGTTGATAAGGTTA+TGG	-	chr3.2:15402423-15402442	None:intergenic	35.0%
	ATGGTACAAATCGATCGACA+AGG	+	chr3.2:15402365-15402384	MS.gene94527:CDS	40.0%
	GATACCGATCAGAATTTCGT+TGG	-	chr3.2:15402474-15402493	None:intergenic	40.0%
	GATGTGTTCCATTTGTTTGG+CGG	+	chr3.2:15402537-15402556	MS.gene94527:CDS	40.0%
	GGAACAATTTGACGACACAA+AGG	-	chr3.2:15402658-15402677	None:intergenic	40.0%
!	TCTCGTTGATAAGGTTATGG+TGG	-	chr3.2:15402420-15402439	None:intergenic	40.0%
	CAAAAGCTCCGCCAAACAAA+TGG	-	chr3.2:15402548-15402567	None:intergenic	45.0%
	TCGTGGTACAAATGCGAACA+CGG	-	chr3.2:15402595-15402614	None:intergenic	45.0%
!	CATTTGTTTGGCGGAGCTTT+TGG	+	chr3.2:15402546-15402565	MS.gene94527:CDS	45.0%
!	TTATGGTGGCGAACAACAGA+AGG	-	chr3.2:15402406-15402425	None:intergenic	45.0%
	TGGTTGGAACGCAAGCAACT+CGG	+	chr3.2:15402566-15402585	MS.gene94527:CDS	50.0%
!	TGTTTGGCGGAGCTTTTGGT+TGG	+	chr3.2:15402550-15402569	MS.gene94527:CDS	50.0%
	GCTCGTCGTCGTCATACTCA+TGG	+	chr3.2:15402346-15402365	MS.gene94527:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	15402259	15402687	15402259	ID=MS.gene94527
chr3.2	mRNA	15402259	15402687	15402259	ID=MS.gene94527.t1;Parent=MS.gene94527
chr3.2	exon	15402259	15402687	15402259	ID=MS.gene94527.t1.exon1;Parent=MS.gene94527.t1
chr3.2	CDS	15402259	15402687	15402259	ID=cds.MS.gene94527.t1;Parent=MS.gene94527.t1

Gene Sequence

>MS.gene94527

ATGGAGCAACAACCACGTAATATAAGAAGTGCTATAAATGTAGAAAGTGATTATGTAATGATTCTCGATGCTGTTGATCTAAATCTTGCTCGTCGTCGTCATACTCATGGTACAAATCGATCGACAAGGTTCACTCTCTCGCGTCCTTCTGTTGTTCGCCACCATAACCTTATCAACGAGATTCAAGCTACTACAAGAAATACTGAAACTATCCAACGAAATTCTGATCGGTATCTTTCAAAAATTAGAGTTGAAGAAGGAAAGGAGTCTGAAGCTATGATGTGTTCCATTTGTTTGGCGGAGCTTTTGGTTGGAACGCAAGCAACTCGGTTGCCGTGTTCGCATTTGTACCACGAAGAATGTATCATGAAATGGTTTGGTAGATCAAACACTTGCCCTTTGTGTCGTCAAATTGTTCCTAATATGTGA

Protein sequence

>MS.gene94527.t1

MEQQPRNIRSAINVESDYVMILDAVDLNLARRRHTHGTNRSTRFTLSRPSVVRHHNLINEIQATTRNTETIQRNSDRYLSKIRVEEGKESEAMMCSICLAELLVGTQATRLPCSHLYHEECIMKWFGRSNTCPLCRQIVPNM