AlfalfaGEDB | MS.gene041075

M. sativa cultivar XinJiangDaYe / MS.gene041075

KEGG: -
GO: -
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041075.t1	AET00676.1	56	218	43	2	1	165	1	218	7.80E-58	233.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041075.t1	P93823	34.6	81	44	3	87	165	103	176	3.5e-06	53.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041075.t1	G7K4G0	56.0	218	43	2	1	165	1	218	5.6e-58	233.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene041075	MS.gene017970	PPI
MS.gene041075	MS.gene055988	PPI
MS.gene94527	MS.gene041075	PPI
MS.gene28215	MS.gene041075	PPI
MS.gene94532	MS.gene041075	PPI
MS.gene065402	MS.gene041075	PPI
MS.gene97792	MS.gene041075	PPI
MS.gene041075	MS.gene010008	PPI
MS.gene43652	MS.gene041075	PPI
MS.gene61920	MS.gene041075	PPI
MS.gene047623	MS.gene041075	PPI
MS.gene61922	MS.gene041075	PPI
MS.gene43658	MS.gene041075	PPI
MS.gene28213	MS.gene041075	PPI
MS.gene029745	MS.gene041075	PPI
MS.gene041075	MS.gene94530	PPI
MS.gene93487	MS.gene041075	PPI
MS.gene47429	MS.gene041075	PPI
MS.gene041075	MS.gene010006	PPI
MS.gene017995	MS.gene041075	PPI
MS.gene041075	MS.gene61924	PPI
MS.gene041075	MS.gene94537	PPI
MS.gene015160	MS.gene041075	PPI
MS.gene041075	MS.gene023764	PPI
MS.gene010343	MS.gene041075	PPI
MS.gene79417	MS.gene041075	PPI
MS.gene041075	MS.gene94533	PPI
MS.gene61921	MS.gene041075	PPI
MS.gene041075	MS.gene023766	PPI
MS.gene041075	MS.gene52667	PPI
MS.gene065401	MS.gene041075	PPI
MS.gene93489	MS.gene041075	PPI
MS.gene041075	MS.gene28214	PPI
MS.gene79416	MS.gene041075	PPI
MS.gene041075	MS.gene051416	PPI
MS.gene041075	MS.gene93488	PPI
MS.gene94534	MS.gene041075	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041075.t1	MTR_5g094680	55.455	220	45	2	1	167	1	220	5.89e-77	229
MS.gene041075.t1	MTR_7g093310	55.944	143	38	2	25	167	20	137	3.09e-46	148
MS.gene041075.t1	MTR_7g092960	65.714	105	27	2	61	165	1	96	7.87e-43	138
MS.gene041075.t1	MTR_8g085550	36.641	131	77	2	42	166	18	148	1.04e-18	78.6
MS.gene041075.t1	MTR_5g006240	32.432	148	86	3	29	165	79	223	1.21e-18	80.1
MS.gene041075.t1	MTR_1g029860	31.343	134	75	4	37	166	56	176	4.46e-18	77.4
MS.gene041075.t1	MTR_8g085520	35.971	139	82	3	35	166	10	148	4.88e-18	77.0
MS.gene041075.t1	MTR_5g037840	35.433	127	62	3	58	166	5	129	1.92e-17	74.7
MS.gene041075.t1	MTR_5g037810	33.858	127	64	2	58	166	5	129	3.34e-17	73.9
MS.gene041075.t1	MTR_8g085530	51.923	52	25	0	115	166	245	296	9.84e-13	65.1
MS.gene041075.t1	MTR_1g029910	28.834	163	89	6	16	166	45	192	1.86e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 29 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCCCAAAAGATTATCCTTT+TGG	0.239033	5.2:+9312371	MS.gene041075:CDS
TTACCAAAAGGATAATCTTT+TGG	0.274956	5.2:-9312374	None:intergenic
TTTGATATGTGAGAAGAAAT+AGG	0.329663	5.2:-9312569	None:intergenic
ACGACGAAGATCAGAAAATC+TGG	0.344881	5.2:+9312642	MS.gene041075:CDS
GGACCGGTGTGCAATTTGTT+TGG	0.399227	5.2:+9312787	MS.gene041075:CDS
TTGTCGTGGATAGGTTGAAA+AGG	0.455255	5.2:+9312732	MS.gene041075:CDS
CTCAGATTGGTCAACATGCT+AGG	0.500915	5.2:+9312603	MS.gene041075:CDS
TAGGTTGAAAAGGGTTGGAA+TGG	0.524218	5.2:+9312742	MS.gene041075:CDS
GTGGATAGGTTGAAAAGGGT+TGG	0.528714	5.2:+9312737	MS.gene041075:CDS
AAAGAAATACTGCCTCAGAT+TGG	0.530797	5.2:+9312590	MS.gene041075:CDS
TGTCGTGGATAGGTTGAAAA+GGG	0.534622	5.2:+9312733	MS.gene041075:CDS
TCAACCTATCCACGACAAGT+AGG	0.536681	5.2:-9312727	None:intergenic
CAACCTATCCACGACAAGTA+GGG	0.541054	5.2:-9312726	None:intergenic
TGTTTGGAAGAGCTCTTCGA+TGG	0.552068	5.2:+9312803	MS.gene041075:CDS
GTGAGAAGAAATAGGCAGAG+AGG	0.554494	5.2:-9312561	None:intergenic
TCATTCATCTCGTTACTCTA+CGG	0.565153	5.2:+9312766	MS.gene041075:CDS
CGACGAAGATCAGAAAATCT+GGG	0.572391	5.2:+9312643	MS.gene041075:CDS
AGGCCCTACTTGTCGTGGAT+AGG	0.585391	5.2:+9312723	MS.gene041075:CDS
TCAGATTGGTCAACATGCTA+GGG	0.585909	5.2:+9312604	MS.gene041075:CDS
TGGTCAACATGCTAGGGAGA+TGG	0.591832	5.2:+9312610	MS.gene041075:CDS
GCGACACAATGGACACGAGA+GGG	0.606474	5.2:-9312921	None:intergenic
TGAAGATAATGATGCTGTGA+AGG	0.607686	5.2:+9312703	MS.gene041075:CDS
TGAAGCTTGATGTCAATACA+TGG	0.634786	5.2:+9312675	MS.gene041075:CDS
CATCTCGTTACTCTACGGAC+CGG	0.636560	5.2:+9312771	MS.gene041075:CDS
CTAGCATGTTGACCAATCTG+AGG	0.646175	5.2:-9312602	None:intergenic
TGCGACACAATGGACACGAG+AGG	0.660737	5.2:-9312922	None:intergenic
ACGAGAGGGATGATTGACGA+TGG	0.663362	5.2:-9312907	None:intergenic
TGTGAAGGCCCTACTTGTCG+TGG	0.681329	5.2:+9312718	MS.gene041075:CDS
CTTCCAAACAAATTGCACAC+CGG	0.732526	5.2:-9312790	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATAAAGAGTGTATTTTTCAA+TGG	+	chr5.2:9312870-9312889	MS.gene041075:CDS	20.0%
!	ATATGGAAATAGTTACCAAA+AGG	-	chr5.2:9312389-9312408	None:intergenic	25.0%
!	ATGAAAAACATGCAAACAAT+TGG	-	chr5.2:9312852-9312871	None:intergenic	25.0%
!	CTATCTAATAAGATATCACA+AGG	-	chr5.2:9312509-9312528	None:intergenic	25.0%
!	CTTGTGATATCTTATTAGAT+AGG	+	chr5.2:9312507-9312526	MS.gene041075:intron	25.0%
!	TTTGATATGTGAGAAGAAAT+AGG	-	chr5.2:9312572-9312591	None:intergenic	25.0%
!!	TACCAAAAGGATAATCTTTT+GGG	-	chr5.2:9312376-9312395	None:intergenic	25.0%
!!	TTACCAAAAGGATAATCTTT+TGG	-	chr5.2:9312377-9312396	None:intergenic	25.0%
!!!	ATAATCTTTTGGGATTGTAA+AGG	-	chr5.2:9312366-9312385	None:intergenic	25.0%
	AATGCAAGGGAAAATTAACA+AGG	-	chr5.2:9312427-9312446	None:intergenic	30.0%
	GAAAATTAACAAGGTCGTAA+TGG	-	chr5.2:9312418-9312437	None:intergenic	30.0%
	TACTATAAATCGAACCTTGT+TGG	+	chr5.2:9312481-9312500	MS.gene041075:intron	30.0%
!	ATCCCAAAAGATTATCCTTT+TGG	+	chr5.2:9312371-9312390	MS.gene041075:CDS	30.0%
!	GGTCGTAATGGAAATTAATA+TGG	-	chr5.2:9312406-9312425	None:intergenic	30.0%
	AAAGAAATACTGCCTCAGAT+TGG	+	chr5.2:9312590-9312609	MS.gene041075:CDS	35.0%
	TCATTCATCTCGTTACTCTA+CGG	+	chr5.2:9312766-9312785	MS.gene041075:CDS	35.0%
	TGAAGATAATGATGCTGTGA+AGG	+	chr5.2:9312703-9312722	MS.gene041075:CDS	35.0%
	TGAAGCTTGATGTCAATACA+TGG	+	chr5.2:9312675-9312694	MS.gene041075:CDS	35.0%
	TTATAGTAGTCGTACAGTCA+AGG	-	chr5.2:9312469-9312488	None:intergenic	35.0%
!!!	TCTTTTGGGATTGTAAAGGT+TGG	-	chr5.2:9312362-9312381	None:intergenic	35.0%
	ACAGTCAAGGATTGAGTTTG+AGG	-	chr5.2:9312456-9312475	None:intergenic	40.0%
	ACGACGAAGATCAGAAAATC+TGG	+	chr5.2:9312642-9312661	MS.gene041075:CDS	40.0%
	CGACGAAGATCAGAAAATCT+GGG	+	chr5.2:9312643-9312662	MS.gene041075:CDS	40.0%
	CTTCCAAACAAATTGCACAC+CGG	-	chr5.2:9312793-9312812	None:intergenic	40.0%
	GATATCACAAGGAACCAACA+AGG	-	chr5.2:9312498-9312517	None:intergenic	40.0%
!	AAGGATTGAGTTTGAGGCAT+TGG	-	chr5.2:9312450-9312469	None:intergenic	40.0%
!	TAGGTTGAAAAGGGTTGGAA+TGG	+	chr5.2:9312742-9312761	MS.gene041075:CDS	40.0%
!	TCAGATTGGTCAACATGCTA+GGG	+	chr5.2:9312604-9312623	MS.gene041075:CDS	40.0%
!	TCATCAGTTTTGCGACACAA+TGG	-	chr5.2:9312935-9312954	None:intergenic	40.0%
!	TGTCGTGGATAGGTTGAAAA+GGG	+	chr5.2:9312733-9312752	MS.gene041075:CDS	40.0%
!	TTGTCGTGGATAGGTTGAAA+AGG	+	chr5.2:9312732-9312751	MS.gene041075:CDS	40.0%
!!	TTTGAGGCATTGGAATGCAA+GGG	-	chr5.2:9312440-9312459	None:intergenic	40.0%
	CAACCTATCCACGACAAGTA+GGG	-	chr5.2:9312729-9312748	None:intergenic	45.0%
	CTAGCATGTTGACCAATCTG+AGG	-	chr5.2:9312605-9312624	None:intergenic	45.0%
	GTGAGAAGAAATAGGCAGAG+AGG	-	chr5.2:9312564-9312583	None:intergenic	45.0%
	TCAACCTATCCACGACAAGT+AGG	-	chr5.2:9312730-9312749	None:intergenic	45.0%
	TGTTTGGAAGAGCTCTTCGA+TGG	+	chr5.2:9312803-9312822	MS.gene041075:CDS	45.0%
!	CTCAGATTGGTCAACATGCT+AGG	+	chr5.2:9312603-9312622	MS.gene041075:CDS	45.0%
!	GTGGATAGGTTGAAAAGGGT+TGG	+	chr5.2:9312737-9312756	MS.gene041075:CDS	45.0%
!!	GTTTGAGGCATTGGAATGCA+AGG	-	chr5.2:9312441-9312460	None:intergenic	45.0%
	ACGAGAGGGATGATTGACGA+TGG	-	chr5.2:9312910-9312929	None:intergenic	50.0%
	CATCTCGTTACTCTACGGAC+CGG	+	chr5.2:9312771-9312790	MS.gene041075:CDS	50.0%
	TGGTCAACATGCTAGGGAGA+TGG	+	chr5.2:9312610-9312629	MS.gene041075:CDS	50.0%
!	GGACCGGTGTGCAATTTGTT+TGG	+	chr5.2:9312787-9312806	MS.gene041075:CDS	50.0%
	AGGCCCTACTTGTCGTGGAT+AGG	+	chr5.2:9312723-9312742	MS.gene041075:CDS	55.0%
	GCGACACAATGGACACGAGA+GGG	-	chr5.2:9312924-9312943	None:intergenic	55.0%
	TGCGACACAATGGACACGAG+AGG	-	chr5.2:9312925-9312944	None:intergenic	55.0%
	TGTGAAGGCCCTACTTGTCG+TGG	+	chr5.2:9312718-9312737	MS.gene041075:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	9312317	9312967	9312317	ID=MS.gene041075
chr5.2	mRNA	9312317	9312967	9312317	ID=MS.gene041075.t1;Parent=MS.gene041075
chr5.2	exon	9312317	9312392	9312317	ID=MS.gene041075.t1.exon1;Parent=MS.gene041075.t1
chr5.2	CDS	9312317	9312392	9312317	ID=cds.MS.gene041075.t1;Parent=MS.gene041075.t1
chr5.2	exon	9312525	9312967	9312525	ID=MS.gene041075.t1.exon2;Parent=MS.gene041075.t1
chr5.2	CDS	9312525	9312967	9312525	ID=cds.MS.gene041075.t1;Parent=MS.gene041075.t1

Gene Sequence

>MS.gene041075

ATGAATTATAATTTTCGCTTGAAAATAAGTTCAATCGAAGAACCAACCTTTACAATCCCAAAAGATTATCCTTTTGGTAACTATTTCCATATTAATTTCCATTACGACCTTGTTAATTTTCCCTTGCATTCCAATGCCTCAAACTCAATCCTTGACTGTACGACTACTATAAATCGAACCTTGTTGGTTCCTTGTGATATCTTATTAGATAGGAAAGACAACATCTTATATGAGTACTATTTTTCCTCTCTGCCTATTTCTTCTCACATATCAAAAGAAATACTGCCTCAGATTGGTCAACATGCTAGGGAGATGGTCGCTAATGACGACGAAGATCAGAAAATCTGGGAGATAGACGTGAAGCTTGATGTCAATACATGGTATATTGAAGATAATGATGCTGTGAAGGCCCTACTTGTCGTGGATAGGTTGAAAAGGGTTGGAATGGATCATTCATCTCGTTACTCTACGGACCGGTGTGCAATTTGTTTGGAAGAGCTCTTCGATGGATCAAAGTCAGAACATGTTATGACCAATTGTTTGCATGTTTTTCATAAAGAGTGTATTTTTCAATGGCTCAAAAGATGCTTCCATCGTCAATCATCCCTCTCGTGTCCATTGTGTCGCAAAACTGATGAAGCTTTTGAATGA

Protein sequence

>MS.gene041075.t1

MNYNFRLKISSIEEPTFTIPKDYPFDRKDNILYEYYFSSLPISSHISKEILPQIGQHAREMVANDDEDQKIWEIDVKLDVNTWYIEDNDAVKALLVVDRLKRVGMDHSSRYSTDRCAICLEELFDGSKSEHVMTNCLHVFHKECIFQWLKRCFHRQSSLSCPLCRKTDEAFE