Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28213.t1 | AES69216.1 | 88.3 | 77 | 9 | 0 | 1 | 77 | 1 | 77 | 4.60E-31 | 143.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28213.t1 | G7IZ80 | 88.3 | 77 | 9 | 0 | 1 | 77 | 1 | 77 | 3.3e-31 | 143.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28213.t1 | MTR_3g025510 | 88.312 | 77 | 9 | 0 | 1 | 77 | 1 | 77 | 8.49e-46 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACGACTCGGTCATGATTT+TGG | 0.175603 | 3.3:+17446480 | MS.gene28213:CDS |
| ACGACTACTCCATATGTTGA+TGG | 0.440424 | 3.3:+17446592 | MS.gene28213:CDS |
| ATATAGTGGCCATCAACATA+TGG | 0.473428 | 3.3:-17446601 | None:intergenic |
| TAAGAGTGTGATAATGAGGA+GGG | 0.483867 | 3.3:-17446522 | None:intergenic |
| AGAGTGTGATAATGAGGAGG+GGG | 0.485557 | 3.3:-17446520 | None:intergenic |
| CTAGTAAGAGTGTGATAATG+AGG | 0.496331 | 3.3:-17446526 | None:intergenic |
| TTACTAGAAATTCATCAAGA+AGG | 0.507286 | 3.3:+17446542 | MS.gene28213:CDS |
| CCAAGAGTCAAAATTGAAGA+AGG | 0.508189 | 3.3:+17446702 | MS.gene28213:CDS |
| TGATGACATGTAAAATATAG+TGG | 0.527734 | 3.3:-17446615 | None:intergenic |
| GTAAGAGTGTGATAATGAGG+AGG | 0.560718 | 3.3:-17446523 | None:intergenic |
| TAGTATGTTTCTCGTTGATG+TGG | 0.599775 | 3.3:-17446639 | None:intergenic |
| AAGAGTGTGATAATGAGGAG+GGG | 0.625865 | 3.3:-17446521 | None:intergenic |
| GTTCAACGAAGACAACGACT+CGG | 0.746473 | 3.3:+17446468 | MS.gene28213:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGATGACATGTAAAATATAG+TGG | - | chr3.3:17446618-17446637 | None:intergenic | 25.0% |
| ! | TTACTAGAAATTCATCAAGA+AGG | + | chr3.3:17446542-17446561 | MS.gene28213:CDS | 25.0% |
| ATATAGTGGCCATCAACATA+TGG | - | chr3.3:17446604-17446623 | None:intergenic | 35.0% | |
| CCAAGAGTCAAAATTGAAGA+AGG | + | chr3.3:17446702-17446721 | MS.gene28213:CDS | 35.0% | |
| TAGTATGTTTCTCGTTGATG+TGG | - | chr3.3:17446642-17446661 | None:intergenic | 35.0% | |
| ! | CTAGTAAGAGTGTGATAATG+AGG | - | chr3.3:17446529-17446548 | None:intergenic | 35.0% |
| ! | TAAGAGTGTGATAATGAGGA+GGG | - | chr3.3:17446525-17446544 | None:intergenic | 35.0% |
| !!! | CCTTCTTCAATTTTGACTCT+TGG | - | chr3.3:17446705-17446724 | None:intergenic | 35.0% |
| ACGACTACTCCATATGTTGA+TGG | + | chr3.3:17446592-17446611 | MS.gene28213:CDS | 40.0% | |
| ! | AAGAGTGTGATAATGAGGAG+GGG | - | chr3.3:17446524-17446543 | None:intergenic | 40.0% |
| ! | GTAAGAGTGTGATAATGAGG+AGG | - | chr3.3:17446526-17446545 | None:intergenic | 40.0% |
| GTTCAACGAAGACAACGACT+CGG | + | chr3.3:17446468-17446487 | MS.gene28213:CDS | 45.0% | |
| ! | AGAGTGTGATAATGAGGAGG+GGG | - | chr3.3:17446523-17446542 | None:intergenic | 45.0% |
| !!! | CAACGACTCGGTCATGATTT+TGG | + | chr3.3:17446480-17446499 | MS.gene28213:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 17446433 | 17446729 | 17446433 | ID=MS.gene28213 |
| chr3.3 | mRNA | 17446433 | 17446729 | 17446433 | ID=MS.gene28213.t1;Parent=MS.gene28213 |
| chr3.3 | exon | 17446433 | 17446729 | 17446433 | ID=MS.gene28213.t1.exon1;Parent=MS.gene28213.t1 |
| chr3.3 | CDS | 17446433 | 17446729 | 17446433 | ID=cds.MS.gene28213.t1;Parent=MS.gene28213.t1 |
| Gene Sequence |
| Protein sequence |
