AlfalfaGEDB | MS.gene94530

M. sativa cultivar XinJiangDaYe / MS.gene94530

KEGG: -
GO: GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94530.t1	AES69216.1	86.4	154	20	1	1	153	1	154	1.10E-71	279.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94530.t1	Q6AVN2	37.0	92	50	2	57	145	154	240	3.2e-11	69.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94530.t1	G7IZ80	86.4	154	20	1	1	153	1	154	8.0e-72	279.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene015160	MS.gene94530	PPI
MS.gene017970	MS.gene94530	PPI
MS.gene79416	MS.gene94530	PPI
MS.gene055988	MS.gene94530	PPI
MS.gene94534	MS.gene94530	PPI
MS.gene94527	MS.gene94530	PPI
MS.gene28215	MS.gene94530	PPI
MS.gene94530	MS.gene023764	PPI
MS.gene94533	MS.gene94530	PPI
MS.gene94532	MS.gene94530	PPI
MS.gene010008	MS.gene94530	PPI
MS.gene065402	MS.gene94530	PPI
MS.gene041075	MS.gene94530	PPI
MS.gene97792	MS.gene94530	PPI
MS.gene43652	MS.gene94530	PPI
MS.gene010006	MS.gene94530	PPI
MS.gene61920	MS.gene94530	PPI
MS.gene94530	MS.gene023766	PPI
MS.gene94530	MS.gene52667	PPI
MS.gene047623	MS.gene94530	PPI
MS.gene28214	MS.gene94530	PPI
MS.gene61922	MS.gene94530	PPI
MS.gene43658	MS.gene94530	PPI
MS.gene28213	MS.gene94530	PPI
MS.gene029745	MS.gene94530	PPI
MS.gene93487	MS.gene94530	PPI
MS.gene94530	MS.gene28214	PPI
MS.gene47429	MS.gene94530	PPI
MS.gene61924	MS.gene94530	PPI
MS.gene017995	MS.gene94530	PPI
MS.gene52667	MS.gene94530	PPI
MS.gene010343	MS.gene94530	PPI
MS.gene79417	MS.gene94530	PPI
MS.gene61921	MS.gene94530	PPI
MS.gene065401	MS.gene94530	PPI
MS.gene93488	MS.gene94530	PPI
MS.gene051416	MS.gene94530	PPI
MS.gene94537	MS.gene94530	PPI
MS.gene93489	MS.gene94530	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94530.t1	MTR_3g025510	86.364	154	20	1	1	153	1	154	4.07e-96	274
MS.gene94530.t1	MTR_3g025380	49.669	151	60	5	1	148	1	138	4.01e-30	106
MS.gene94530.t1	MTR_3g025550	74.074	54	14	0	93	146	6	59	3.83e-24	89.4
MS.gene94530.t1	MTR_3g025620	67.308	52	17	0	97	148	1	52	4.21e-20	78.6
MS.gene94530.t1	MTR_2g058360	51.899	79	32	3	74	148	64	140	8.84e-18	75.1
MS.gene94530.t1	MTR_7g074170	39.785	93	52	2	57	145	294	386	2.96e-16	75.1
MS.gene94530.t1	MTR_6g079660	33.333	93	58	2	57	145	184	276	7.65e-15	70.9
MS.gene94530.t1	MTR_1g114320	28.205	117	80	2	36	148	122	238	1.01e-13	67.4
MS.gene94530.t1	MTR_4g108830	44.776	67	36	1	83	148	125	191	1.59e-13	65.1
MS.gene94530.t1	MTR_4g108830	44.776	67	36	1	83	148	153	219	2.09e-13	65.5
MS.gene94530.t1	MTR_3g083480	33.684	95	55	2	57	148	192	281	2.77e-13	66.2
MS.gene94530.t1	MTR_1g114620	36.559	93	53	2	57	145	215	305	3.62e-13	65.9
MS.gene94530.t1	MTR_3g072630	36.559	93	58	1	57	148	173	265	5.23e-13	65.5
MS.gene94530.t1	MTR_3g088080	35.802	81	46	3	66	145	4	79	5.91e-13	61.2
MS.gene94530.t1	MTR_3g072630	36.559	93	58	1	57	148	173	265	6.56e-13	65.1
MS.gene94530.t1	MTR_2g097620	38.333	60	37	0	86	145	171	230	7.48e-12	62.0
MS.gene94530.t1	MTR_5g058530	48.980	49	25	0	97	145	217	265	9.85e-12	61.6
MS.gene94530.t1	MTR_5g041330	33.333	90	55	2	58	145	60	146	1.29e-11	60.5
MS.gene94530.t1	MTR_0067s0100	30.693	101	70	0	45	145	131	231	1.61e-11	60.8
MS.gene94530.t1	MTR_0186s0090	48.000	50	23	1	103	149	115	164	2.08e-11	58.9
MS.gene94530.t1	MTR_5g041310	43.103	58	30	1	88	145	102	156	2.39e-11	59.7
MS.gene94530.t1	MTR_3g087900	51.064	47	22	1	104	149	69	115	2.74e-11	58.5
MS.gene94530.t1	MTR_4g064570	43.750	64	34	2	86	148	163	225	2.99e-11	60.5
MS.gene94530.t1	MTR_8g056930	48.000	50	23	1	103	149	115	164	3.03e-11	58.5
MS.gene94530.t1	MTR_4g110100	44.262	61	33	1	86	145	341	401	5.08e-11	59.7
MS.gene94530.t1	MTR_3g081030	34.247	73	47	1	77	148	92	164	5.87e-11	58.5
MS.gene94530.t1	MTR_6g016350	50.000	44	22	0	102	145	309	352	7.09e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94530.t1	AT4G26400	34.951	103	59	2	46	145	185	282	1.98e-15	72.4
MS.gene94530.t1	AT4G26400	34.951	103	59	2	46	145	185	282	1.98e-15	72.4
MS.gene94530.t1	AT5G08139	46.154	65	34	1	82	145	286	350	7.75e-15	70.9
MS.gene94530.t1	AT1G14200	37.333	75	47	0	71	145	76	150	1.57e-13	65.1
MS.gene94530.t1	AT5G15820	35.789	95	54	3	58	145	238	332	3.31e-13	65.9
MS.gene94530.t1	AT3G19950	31.183	93	60	2	57	145	223	315	5.54e-13	65.5
MS.gene94530.t1	AT3G19950	31.183	93	60	2	57	145	223	315	5.54e-13	65.5
MS.gene94530.t1	AT3G19950	31.183	93	60	2	57	145	165	257	6.11e-13	65.1
MS.gene94530.t1	AT3G02340	37.363	91	52	3	60	145	286	376	6.90e-13	65.1
MS.gene94530.t1	AT3G02340	37.363	91	52	3	60	145	286	376	6.90e-13	65.1
MS.gene94530.t1	AT3G30460	47.619	63	31	1	88	148	115	177	1.40e-12	62.4
MS.gene94530.t1	AT3G30460	47.619	63	31	1	88	148	79	141	1.64e-12	61.6
MS.gene94530.t1	AT4G05350	43.284	67	33	2	88	149	139	205	2.02e-12	62.4
MS.gene94530.t1	AT5G01980	34.783	92	57	1	57	145	300	391	4.45e-12	63.2
MS.gene94530.t1	AT4G12190	43.284	67	33	2	88	149	4	70	5.14e-12	58.2
MS.gene94530.t1	AT1G18780	44.000	50	28	0	96	145	269	318	7.62e-12	62.0
MS.gene94530.t1	AT1G18770	46.512	43	23	0	103	145	58	100	1.09e-11	58.5
MS.gene94530.t1	AT4G12210	41.791	67	34	2	88	149	135	201	1.10e-11	60.5
MS.gene94530.t1	AT3G60080	37.313	67	34	1	87	145	144	210	1.30e-11	61.2
MS.gene94530.t1	AT5G54990	53.333	45	21	0	101	145	171	215	1.31e-11	60.5
MS.gene94530.t1	AT5G56340	32.609	92	54	2	57	145	214	300	1.56e-11	61.2
MS.gene94530.t1	AT2G40830	30.275	109	71	3	49	153	132	239	1.68e-11	61.2
MS.gene94530.t1	AT2G40830	30.275	109	71	3	49	153	132	239	1.68e-11	61.2
MS.gene94530.t1	AT2G40830	30.275	109	71	3	49	153	132	239	1.68e-11	61.2
MS.gene94530.t1	AT2G40830	30.275	109	71	3	49	153	132	239	1.68e-11	61.2
MS.gene94530.t1	AT1G60360	37.681	69	42	1	86	153	205	273	2.12e-11	60.8
MS.gene94530.t1	AT1G55530	30.435	92	56	2	57	145	145	231	2.18e-11	60.8
MS.gene94530.t1	AT1G55530	28.000	100	64	2	49	145	170	264	2.22e-11	60.8
MS.gene94530.t1	AT1G55530	28.000	100	64	2	49	145	170	264	2.22e-11	60.8
MS.gene94530.t1	AT5G60820	50.000	46	23	0	100	145	368	413	3.94e-11	60.1
MS.gene94530.t1	AT3G10815	46.667	45	24	0	104	148	121	165	4.85e-11	58.5
MS.gene94530.t1	AT3G13430	27.723	101	65	2	49	145	170	266	5.75e-11	59.7
MS.gene94530.t1	AT3G13430	27.723	101	65	2	49	145	170	266	5.75e-11	59.7
MS.gene94530.t1	AT3G13430	27.723	101	65	2	49	145	170	266	5.75e-11	59.7
MS.gene94530.t1	AT3G13430	27.723	101	65	2	49	145	170	266	5.75e-11	59.7
MS.gene94530.t1	AT2G29840	47.826	46	24	0	100	145	240	285	5.98e-11	59.3

Find 28 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACGACTCGGTTATGATTT+TGG	0.133349	3.2:+15533953	MS.gene94530:CDS
CCTTCTTCAATTTCGACTCT+TGG	0.354455	3.2:-15534173	None:intergenic
CGAATTGCTTTCGACCCAAC+TGG	0.391692	3.2:-15534242	None:intergenic
GATGTGTTCTATTTGTTTAG+CGG	0.424628	3.2:+15534214	MS.gene94530:CDS
ACGACTACTCCATATGTTGA+TGG	0.440424	3.2:+15534065	MS.gene94530:CDS
CAGTTGGGTCGAAAGCAATT+CGG	0.451135	3.2:+15534243	MS.gene94530:CDS
ACGATGAGTGTATCATGAAA+TGG	0.467296	3.2:+15534288	MS.gene94530:CDS
ATATAGTGGCCATCAACATA+TGG	0.473428	3.2:-15534074	None:intergenic
TGTTTAGCGGAGCTTCCAGT+TGG	0.484304	3.2:+15534227	MS.gene94530:CDS
TGGAGCAACATCGTCGTTCA+AGG	0.491831	3.2:+15533910	MS.gene94530:CDS
ATACGCTCTGACGACACATT+GGG	0.496707	3.2:-15534331	None:intergenic
TTACTAGAAATTCATCAAGA+AGG	0.509458	3.2:+15534015	MS.gene94530:CDS
CTAGTAAAAGTGTGATAATG+AGG	0.517777	3.2:-15533999	None:intergenic
TGATGACATGTAAAATATAG+TGG	0.527734	3.2:-15534088	None:intergenic
CCAAGAGTCGAAATTGAAGA+AGG	0.529637	3.2:+15534173	MS.gene94530:CDS
GTTTAGCGGAGCTTCCAGTT+GGG	0.530051	3.2:+15534228	MS.gene94530:CDS
TAAAAGTGTGATAATGAGGA+GGG	0.533579	3.2:-15533995	None:intergenic
AATACGCTCTGACGACACAT+TGG	0.540627	3.2:-15534332	None:intergenic
AAAGTGTGATAATGAGGAGG+GGG	0.541531	3.2:-15533993	None:intergenic
AACCGAGTCGTTGTCTTCAG+CGG	0.564335	3.2:-15533943	None:intergenic
TGAACGACGATGTTGCTCCA+TGG	0.567207	3.2:-15533907	None:intergenic
TCGTGGTAGGTATGCGAGCA+CGG	0.574818	3.2:-15534269	None:intergenic
TAGTATGATTCTCGTTGATG+TGG	0.592252	3.2:-15534112	None:intergenic
GTAAAAGTGTGATAATGAGG+AGG	0.601014	3.2:-15533996	None:intergenic
CATGATACACTCATCGTGGT+AGG	0.601526	3.2:-15534282	None:intergenic
AAAAGTGTGATAATGAGGAG+GGG	0.621648	3.2:-15533994	None:intergenic
GTCCGCTGAAGACAACGACT+CGG	0.678762	3.2:+15533941	MS.gene94530:CDS
ATTTCATGATACACTCATCG+TGG	0.739086	3.2:-15534286	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGATGACATGTAAAATATAG+TGG	-	chr3.2:15534091-15534110	None:intergenic	25.0%
!	TTACTAGAAATTCATCAAGA+AGG	+	chr3.2:15534015-15534034	MS.gene94530:CDS	25.0%
	GATGTGTTCTATTTGTTTAG+CGG	+	chr3.2:15534214-15534233	MS.gene94530:CDS	30.0%
!	CTAGTAAAAGTGTGATAATG+AGG	-	chr3.2:15534002-15534021	None:intergenic	30.0%
!	TAAAAGTGTGATAATGAGGA+GGG	-	chr3.2:15533998-15534017	None:intergenic	30.0%
	ACGATGAGTGTATCATGAAA+TGG	+	chr3.2:15534288-15534307	MS.gene94530:CDS	35.0%
	ATATAGTGGCCATCAACATA+TGG	-	chr3.2:15534077-15534096	None:intergenic	35.0%
	ATTTCATGATACACTCATCG+TGG	-	chr3.2:15534289-15534308	None:intergenic	35.0%
	TAGTATGATTCTCGTTGATG+TGG	-	chr3.2:15534115-15534134	None:intergenic	35.0%
!	AAAAGTGTGATAATGAGGAG+GGG	-	chr3.2:15533997-15534016	None:intergenic	35.0%
!	GTAAAAGTGTGATAATGAGG+AGG	-	chr3.2:15533999-15534018	None:intergenic	35.0%
	ACGACTACTCCATATGTTGA+TGG	+	chr3.2:15534065-15534084	MS.gene94530:CDS	40.0%
	CCAAGAGTCGAAATTGAAGA+AGG	+	chr3.2:15534173-15534192	MS.gene94530:CDS	40.0%
	CCTTCTTCAATTTCGACTCT+TGG	-	chr3.2:15534176-15534195	None:intergenic	40.0%
!	AAAGTGTGATAATGAGGAGG+GGG	-	chr3.2:15533996-15534015	None:intergenic	40.0%
!!!	CAACGACTCGGTTATGATTT+TGG	+	chr3.2:15533953-15533972	MS.gene94530:CDS	40.0%
	AATACGCTCTGACGACACAT+TGG	-	chr3.2:15534335-15534354	None:intergenic	45.0%
	ATACGCTCTGACGACACATT+GGG	-	chr3.2:15534334-15534353	None:intergenic	45.0%
	CAGTTGGGTCGAAAGCAATT+CGG	+	chr3.2:15534243-15534262	MS.gene94530:CDS	45.0%
	CATGATACACTCATCGTGGT+AGG	-	chr3.2:15534285-15534304	None:intergenic	45.0%
	AACCGAGTCGTTGTCTTCAG+CGG	-	chr3.2:15533946-15533965	None:intergenic	50.0%
	CGAATTGCTTTCGACCCAAC+TGG	-	chr3.2:15534245-15534264	None:intergenic	50.0%
	GTTTAGCGGAGCTTCCAGTT+GGG	+	chr3.2:15534228-15534247	MS.gene94530:CDS	50.0%
	TGGAGCAACATCGTCGTTCA+AGG	+	chr3.2:15533910-15533929	MS.gene94530:CDS	50.0%
	TGTTTAGCGGAGCTTCCAGT+TGG	+	chr3.2:15534227-15534246	MS.gene94530:CDS	50.0%
	GTCCGCTGAAGACAACGACT+CGG	+	chr3.2:15533941-15533960	MS.gene94530:CDS	55.0%
	TCGTGGTAGGTATGCGAGCA+CGG	-	chr3.2:15534272-15534291	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	15533909	15534370	15533909	ID=MS.gene94530
chr3.2	mRNA	15533909	15534370	15533909	ID=MS.gene94530.t1;Parent=MS.gene94530
chr3.2	exon	15533909	15534370	15533909	ID=MS.gene94530.t1.exon1;Parent=MS.gene94530.t1
chr3.2	CDS	15533909	15534370	15533909	ID=cds.MS.gene94530.t1;Parent=MS.gene94530.t1

Gene Sequence

>MS.gene94530

ATGGAGCAACATCGTCGTTCAAGGAGAAGTGTGTCCGCTGAAGACAACGACTCGGTTATGATTTTGGATGCTATTGATCTAAATCCCCCTCCTCATTATCACACTTTTACTAGAAATTCATCAAGAAGGTTTGCAAGAAATTTTATAAGATCTTCAACGACTACTCCATATGTTGATGGCCACTATATTTTACATGTCATCAACCACATCAACGAGAATCATACTAGTAGAAGAAATAGAGTCAGAGATATTTATCACAATCTTCCAAGAGTCGAAATTGAAGAAGGAATGAAGTGTGAAGCTTTGATGTGTTCTATTTGTTTAGCGGAGCTTCCAGTTGGGTCGAAAGCAATTCGGTTGCCGTGCTCGCATACCTACCACGATGAGTGTATCATGAAATGGTTAGATAGATCGAACACATGCCCAATGTGTCGTCAGAGCGTATTTCATGTTTCTTCATGA

Protein sequence

>MS.gene94530.t1

MEQHRRSRRSVSAEDNDSVMILDAIDLNPPPHYHTFTRNSSRRFARNFIRSSTTTPYVDGHYILHVINHINENHTSRRNRVRDIYHNLPRVEIEEGMKCEALMCSICLAELPVGSKAIRLPCSHTYHDECIMKWLDRSNTCPMCRQSVFHVSS