AlfalfaGEDB | MS.gene97792

M. sativa cultivar XinJiangDaYe / MS.gene97792

KEGG: -
GO: -
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97792.t1	AES69209.1	82.1	140	25	0	3	142	2	141	4.20E-57	230.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97792.t1	Q5M974	41.9	62	36	0	79	140	63	124	6.6e-11	68.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97792.t1	G7IWF8	82.1	140	25	0	3	142	2	141	3.0e-57	230.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene28215	MS.gene97792	PPI
MS.gene97792	MS.gene017970	PPI
MS.gene94532	MS.gene97792	PPI
MS.gene065402	MS.gene97792	PPI
MS.gene97792	MS.gene055988	PPI
MS.gene43652	MS.gene97792	PPI
MS.gene61920	MS.gene97792	PPI
MS.gene79416	MS.gene97792	PPI
MS.gene97792	MS.gene041075	PPI
MS.gene97792	MS.gene010008	PPI
MS.gene047623	MS.gene97792	PPI
MS.gene61922	MS.gene97792	PPI
MS.gene97792	MS.gene93488	PPI
MS.gene97792	MS.gene94534	PPI
MS.gene43658	MS.gene97792	PPI
MS.gene28213	MS.gene97792	PPI
MS.gene97792	MS.gene94527	PPI
MS.gene93487	MS.gene97792	PPI
MS.gene97792	MS.gene94530	PPI
MS.gene47429	MS.gene97792	PPI
MS.gene97792	MS.gene79417	PPI
MS.gene97792	MS.gene61924	PPI
MS.gene029745	MS.gene97792	PPI
MS.gene97792	MS.gene023764	PPI
MS.gene61921	MS.gene97792	PPI
MS.gene065401	MS.gene97792	PPI
MS.gene97792	MS.gene94533	PPI
MS.gene97792	MS.gene023766	PPI
MS.gene017995	MS.gene97792	PPI
MS.gene93489	MS.gene97792	PPI
MS.gene97792	MS.gene010006	PPI
MS.gene015160	MS.gene97792	PPI
MS.gene97792	MS.gene94537	PPI
MS.gene010343	MS.gene97792	PPI
MS.gene97792	MS.gene28214	PPI
MS.gene97792	MS.gene52667	PPI
MS.gene97792	MS.gene051416	PPI
MS.gene94534	MS.gene97792	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97792.t1	MTR_3g025380	82.143	140	25	0	3	142	2	141	9.06e-73	214
MS.gene97792.t1	MTR_3g025510	46.309	149	70	3	1	139	1	149	1.58e-37	125
MS.gene97792.t1	MTR_3g025550	84.746	59	9	0	84	142	6	64	6.45e-34	113
MS.gene97792.t1	MTR_3g025620	61.111	54	21	0	89	142	2	55	8.62e-20	77.4
MS.gene97792.t1	MTR_3g088080	37.209	86	47	2	56	141	6	84	2.24e-15	67.0
MS.gene97792.t1	MTR_5g041310	36.471	85	50	2	57	140	79	160	2.66e-14	67.4
MS.gene97792.t1	MTR_2g058360	47.619	63	29	2	79	139	80	140	3.30e-14	65.5
MS.gene97792.t1	MTR_7g074170	46.429	56	30	0	85	140	335	390	3.86e-14	68.6
MS.gene97792.t1	MTR_3g072630	31.765	85	57	1	57	140	182	266	3.27e-13	65.5
MS.gene97792.t1	MTR_5g041330	35.294	85	51	2	57	140	69	150	3.39e-13	64.3
MS.gene97792.t1	MTR_3g072630	31.765	85	57	1	57	140	182	266	3.90e-13	65.5
MS.gene97792.t1	MTR_8g056930	41.333	75	40	2	68	139	90	163	1.29e-12	62.0
MS.gene97792.t1	MTR_0186s0090	41.333	75	40	2	68	139	90	163	1.98e-12	61.2
MS.gene97792.t1	MTR_1g114620	36.000	75	46	1	66	140	237	309	7.42e-12	61.6
MS.gene97792.t1	MTR_6g079660	38.462	52	32	0	89	140	229	280	1.09e-11	61.2
MS.gene97792.t1	MTR_4g110100	40.984	61	36	0	80	140	345	405	1.21e-11	61.2
MS.gene97792.t1	MTR_2g097620	41.509	53	30	1	89	141	184	235	1.61e-11	60.8
MS.gene97792.t1	MTR_7g092960	43.333	60	32	1	79	136	37	96	1.76e-11	57.4
MS.gene97792.t1	MTR_1g051105	43.478	46	26	0	95	140	159	204	2.63e-11	59.7
MS.gene97792.t1	MTR_6g016350	43.750	48	27	0	93	140	309	356	4.54e-11	59.7
MS.gene97792.t1	MTR_5g058530	52.381	42	20	0	95	136	224	265	4.79e-11	59.3
MS.gene97792.t1	MTR_2g007340	41.379	58	34	0	79	136	185	242	5.12e-11	59.3
MS.gene97792.t1	MTR_1g114320	39.216	51	31	0	90	140	189	239	6.17e-11	58.9
MS.gene97792.t1	MTR_5g088720	30.667	75	52	0	66	140	217	291	9.29e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97792.t1	AT5G60820	45.283	53	29	0	89	141	366	418	1.05e-13	67.4
MS.gene97792.t1	AT3G02340	53.191	47	22	0	94	140	334	380	1.52e-13	66.6
MS.gene97792.t1	AT3G02340	53.191	47	22	0	94	140	334	380	1.52e-13	66.6
MS.gene97792.t1	AT3G60080	38.028	71	41	1	70	140	147	214	1.53e-13	66.2
MS.gene97792.t1	AT1G60360	47.826	46	24	0	95	140	224	269	1.74e-13	66.2
MS.gene97792.t1	AT5G08139	41.818	55	32	0	86	140	300	354	3.26e-13	65.9
MS.gene97792.t1	AT1G14200	38.710	62	38	0	79	140	93	154	3.35e-13	63.9
MS.gene97792.t1	AT4G12210	47.619	63	28	2	79	136	135	197	3.81e-13	63.9
MS.gene97792.t1	AT3G10815	37.097	62	39	0	79	140	105	166	9.16e-13	62.8
MS.gene97792.t1	AT2G44330	52.174	46	22	0	95	140	96	141	1.14e-12	62.4
MS.gene97792.t1	AT5G15820	47.059	51	27	0	90	140	286	336	1.27e-12	63.9
MS.gene97792.t1	AT1G18770	33.673	98	53	2	55	140	7	104	2.06e-12	60.1
MS.gene97792.t1	AT1G21960	37.500	88	50	2	58	140	116	203	2.45e-12	62.0
MS.gene97792.t1	AT4G05350	36.170	94	49	4	54	136	108	201	3.00e-12	61.6
MS.gene97792.t1	AT4G12190	46.032	63	29	2	79	136	4	66	4.10e-12	58.2
MS.gene97792.t1	AT2G40830	42.553	47	27	0	95	141	190	236	9.37e-12	61.6
MS.gene97792.t1	AT2G40830	42.553	47	27	0	95	141	190	236	9.37e-12	61.6
MS.gene97792.t1	AT2G40830	42.553	47	27	0	95	141	190	236	9.37e-12	61.6
MS.gene97792.t1	AT2G40830	42.553	47	27	0	95	141	190	236	9.37e-12	61.6
MS.gene97792.t1	AT3G30460	44.444	63	33	1	81	141	83	145	9.47e-12	59.3
MS.gene97792.t1	AT3G30460	44.068	59	33	0	81	139	119	177	1.10e-11	59.7
MS.gene97792.t1	AT1G55530	33.871	62	36	1	79	140	212	268	1.34e-11	61.2
MS.gene97792.t1	AT1G55530	33.871	62	36	1	79	140	212	268	1.34e-11	61.2
MS.gene97792.t1	AT1G55530	33.871	62	36	1	79	140	179	235	1.57e-11	60.8
MS.gene97792.t1	AT5G54990	48.889	45	23	0	92	136	171	215	1.64e-11	60.1
MS.gene97792.t1	AT1G18780	46.296	54	29	0	87	140	269	322	2.02e-11	60.5
MS.gene97792.t1	AT3G13430	31.081	74	43	1	75	140	197	270	2.03e-11	60.5
MS.gene97792.t1	AT3G13430	31.081	74	43	1	75	140	197	270	2.03e-11	60.5
MS.gene97792.t1	AT3G13430	31.081	74	43	1	75	140	197	270	2.03e-11	60.5
MS.gene97792.t1	AT3G13430	31.081	74	43	1	75	140	197	270	2.03e-11	60.5
MS.gene97792.t1	AT5G64920	36.207	58	37	0	79	136	241	298	2.77e-11	60.1
MS.gene97792.t1	AT2G17450	32.143	140	83	5	5	139	15	147	3.17e-11	58.5
MS.gene97792.t1	AT4G12140	41.538	65	34	2	79	139	135	199	3.28e-11	58.9
MS.gene97792.t1	AT2G29840	47.059	51	27	0	90	140	239	289	4.00e-11	59.7
MS.gene97792.t1	AT1G18760	45.652	46	25	0	95	140	175	220	6.90e-11	58.2
MS.gene97792.t1	AT1G72310	50.000	50	24	1	91	139	123	172	8.73e-11	58.5

Find 21 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTTGTTTGGCGGAGCTTT+TGG	0.249403	3.4:+17686955	MS.gene97792:CDS
TATGATGTGTTCCATTTGTT+TGG	0.364328	3.4:+17686943	MS.gene97792:CDS
GAATCTCGTTGATAAGGTTA+TGG	0.379677	3.4:-17686829	None:intergenic
CATAAACTAAGTCACATATT+AGG	0.380528	3.4:-17687085	None:intergenic
CAAAAGCTCCGCCAAACAAA+TGG	0.424226	3.4:-17686954	None:intergenic
ACGAAGAATGTATCATGAAA+TGG	0.429864	3.4:+17687020	MS.gene97792:CDS
TAGCTTGAATCTCGTTGATA+AGG	0.444704	3.4:-17686835	None:intergenic
GAATGTATCATGAAATGGTT+TGG	0.470491	3.4:+17687025	MS.gene97792:CDS
ATTTCATGATACATTCTTCG+TGG	0.485172	3.4:-17687018	None:intergenic
AATTAGAGTTGAAGAAGGAA+AGG	0.503387	3.4:+17686910	MS.gene97792:CDS
GATGTGTTCCATTTGTTTGG+CGG	0.523172	3.4:+17686946	MS.gene97792:CDS
GAACAATTTGACGACACAAA+GGG	0.529813	3.4:-17687063	None:intergenic
TCGTGGTACAAATGCGAACA+CGG	0.571244	3.4:-17687001	None:intergenic
GCTCGTCGTCGTCATACTCA+TGG	0.572555	3.4:+17686755	MS.gene97792:CDS
CTATCCAACAAAATTCTGAT+CGG	0.588096	3.4:+17686876	MS.gene97792:CDS
TTATGGTGGCGAACAACAGA+AGG	0.604056	3.4:-17686812	None:intergenic
TGGTTGGAACGCAAGCAACT+CGG	0.615833	3.4:+17686975	MS.gene97792:CDS
GGAACAATTTGACGACACAA+AGG	0.620591	3.4:-17687064	None:intergenic
TCTCGTTGATAAGGTTATGG+TGG	0.632126	3.4:-17686826	None:intergenic
ATAGCACTTCTTATATTACG+TGG	0.670123	3.4:-17686680	None:intergenic
ATGGTACAAATCGATCGACA+AGG	0.741500	3.4:+17686774	MS.gene97792:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCAAAAATTAGAGTTGAAGA+AGG	+	chr3.4:17686905-17686924	MS.gene97792:CDS	25.0%
	AATTAGAGTTGAAGAAGGAA+AGG	+	chr3.4:17686910-17686929	MS.gene97792:CDS	30.0%
	ACGAAGAATGTATCATGAAA+TGG	+	chr3.4:17687020-17687039	MS.gene97792:CDS	30.0%
	ATAGCACTTCTTATATTACG+TGG	-	chr3.4:17686683-17686702	None:intergenic	30.0%
	ATTTCATGATACATTCTTCG+TGG	-	chr3.4:17687021-17687040	None:intergenic	30.0%
	CTATCCAACAAAATTCTGAT+CGG	+	chr3.4:17686876-17686895	MS.gene97792:CDS	30.0%
	GAATGTATCATGAAATGGTT+TGG	+	chr3.4:17687025-17687044	MS.gene97792:CDS	30.0%
	TATGATGTGTTCCATTTGTT+TGG	+	chr3.4:17686943-17686962	MS.gene97792:CDS	30.0%
	GAACAATTTGACGACACAAA+GGG	-	chr3.4:17687066-17687085	None:intergenic	35.0%
	TAGCTTGAATCTCGTTGATA+AGG	-	chr3.4:17686838-17686857	None:intergenic	35.0%
!	GAATCTCGTTGATAAGGTTA+TGG	-	chr3.4:17686832-17686851	None:intergenic	35.0%
!	GATACCGATCAGAATTTTGT+TGG	-	chr3.4:17686883-17686902	None:intergenic	35.0%
	ATGGTACAAATCGATCGACA+AGG	+	chr3.4:17686774-17686793	MS.gene97792:CDS	40.0%
	GATGTGTTCCATTTGTTTGG+CGG	+	chr3.4:17686946-17686965	MS.gene97792:CDS	40.0%
	GGAACAATTTGACGACACAA+AGG	-	chr3.4:17687067-17687086	None:intergenic	40.0%
!	TCTCGTTGATAAGGTTATGG+TGG	-	chr3.4:17686829-17686848	None:intergenic	40.0%
	CAAAAGCTCCGCCAAACAAA+TGG	-	chr3.4:17686957-17686976	None:intergenic	45.0%
	TCGTGGTACAAATGCGAACA+CGG	-	chr3.4:17687004-17687023	None:intergenic	45.0%
!	CATTTGTTTGGCGGAGCTTT+TGG	+	chr3.4:17686955-17686974	MS.gene97792:CDS	45.0%
!	TTATGGTGGCGAACAACAGA+AGG	-	chr3.4:17686815-17686834	None:intergenic	45.0%
	TGGTTGGAACGCAAGCAACT+CGG	+	chr3.4:17686975-17686994	MS.gene97792:CDS	50.0%
!	TGTTTGGCGGAGCTTTTGGT+TGG	+	chr3.4:17686959-17686978	MS.gene97792:CDS	50.0%
	GCTCGTCGTCGTCATACTCA+TGG	+	chr3.4:17686755-17686774	MS.gene97792:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	17686668	17687096	17686668	ID=MS.gene97792
chr3.4	mRNA	17686668	17687096	17686668	ID=MS.gene97792.t1;Parent=MS.gene97792
chr3.4	exon	17686668	17687096	17686668	ID=MS.gene97792.t1.exon1;Parent=MS.gene97792.t1
chr3.4	CDS	17686668	17687096	17686668	ID=cds.MS.gene97792.t1;Parent=MS.gene97792.t1

Gene Sequence

>MS.gene97792

ATGGAGCAACAACCACGTAATATAAGAAGTGCTATAAATGTAGAAAGTGATTATGTAATGATTCTCGATGCTGTTGATCTAAATCTTGCTCGTCGTCGTCATACTCATGGTACAAATCGATCGACAAGGTTCACTCTCTCGCGTCCTTCTGTTGTTCGCCACCATAACCTTATCAACGAGATTCAAGCTACTACAAGAAATACTGAAACTATCCAACAAAATTCTGATCGGTATCTTTCAAAAATTAGAGTTGAAGAAGGAAAGGAGTCTGAAGCTATGATGTGTTCCATTTGTTTGGCGGAGCTTTTGGTTGGAACGCAAGCAACTCGGTTGCCGTGTTCGCATTTGTACCACGAAGAATGTATCATGAAATGGTTTGGTAGATCAAACACTTGCCCTTTGTGTCGTCAAATTGTTCCTAATATGTGA

Protein sequence

>MS.gene97792.t1

MEQQPRNIRSAINVESDYVMILDAVDLNLARRRHTHGTNRSTRFTLSRPSVVRHHNLINEIQATTRNTETIQQNSDRYLSKIRVEEGKESEAMMCSICLAELLVGTQATRLPCSHLYHEECIMKWFGRSNTCPLCRQIVPNM