AlfalfaGEDB | MS.gene93488

M. sativa cultivar XinJiangDaYe / MS.gene93488

KEGG: -
GO: -
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93488.t1	AES69209.1	79.3	140	29	0	3	142	2	141	1.50E-54	222.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93488.t1	Q6GPV5	42.9	63	36	0	78	140	62	124	9.2e-13	74.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93488.t1	G7IWF8	79.3	140	29	0	3	142	2	141	1.1e-54	222.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene61922	MS.gene93488	PPI
MS.gene94534	MS.gene93488	PPI
MS.gene93488	MS.gene023764	PPI
MS.gene94527	MS.gene93488	PPI
MS.gene94532	MS.gene93488	PPI
MS.gene065402	MS.gene93488	PPI
MS.gene97792	MS.gene93488	PPI
MS.gene43652	MS.gene93488	PPI
MS.gene93488	MS.gene023766	PPI
MS.gene010006	MS.gene93488	PPI
MS.gene61920	MS.gene93488	PPI
MS.gene047623	MS.gene93488	PPI
MS.gene61924	MS.gene93488	PPI
MS.gene43658	MS.gene93488	PPI
MS.gene28213	MS.gene93488	PPI
MS.gene029745	MS.gene93488	PPI
MS.gene79417	MS.gene93488	PPI
MS.gene065401	MS.gene93488	PPI
MS.gene93488	MS.gene28214	PPI
MS.gene93488	MS.gene94537	PPI
MS.gene93487	MS.gene93488	PPI
MS.gene93488	MS.gene52667	PPI
MS.gene47429	MS.gene93488	PPI
MS.gene017995	MS.gene93488	PPI
MS.gene015160	MS.gene93488	PPI
MS.gene010343	MS.gene93488	PPI
MS.gene93488	MS.gene017970	PPI
MS.gene61921	MS.gene93488	PPI
MS.gene055988	MS.gene93488	PPI
MS.gene051416	MS.gene93488	PPI
MS.gene94537	MS.gene93488	PPI
MS.gene93489	MS.gene93488	PPI
MS.gene28215	MS.gene93488	PPI
MS.gene010008	MS.gene93488	PPI
MS.gene041075	MS.gene93488	PPI
MS.gene79416	MS.gene93488	PPI
MS.gene93488	MS.gene94530	PPI
MS.gene94533	MS.gene93488	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93488.t1	MTR_3g025380	79.286	140	29	0	3	142	2	141	1.51e-68	203
MS.gene93488.t1	MTR_3g025510	53.020	149	60	4	1	139	1	149	8.46e-43	139
MS.gene93488.t1	MTR_3g025550	86.441	59	8	0	84	142	6	64	2.49e-34	114
MS.gene93488.t1	MTR_3g025620	61.111	54	21	0	89	142	2	55	4.44e-19	75.9
MS.gene93488.t1	MTR_2g058360	48.052	77	35	3	66	139	66	140	8.78e-16	69.7
MS.gene93488.t1	MTR_7g074170	43.750	64	35	1	78	140	327	390	1.55e-15	72.4
MS.gene93488.t1	MTR_3g088080	38.372	86	46	2	56	141	6	84	2.32e-15	67.0
MS.gene93488.t1	MTR_3g072630	40.323	62	37	0	79	140	205	266	9.96e-14	67.0
MS.gene93488.t1	MTR_3g072630	40.323	62	37	0	79	140	205	266	1.18e-13	66.6
MS.gene93488.t1	MTR_1g114320	35.135	74	47	1	68	140	166	239	3.64e-13	65.5
MS.gene93488.t1	MTR_1g114620	38.667	75	44	1	66	140	237	309	3.88e-13	65.5
MS.gene93488.t1	MTR_6g079660	36.842	76	47	1	66	140	205	280	3.99e-13	65.5
MS.gene93488.t1	MTR_5g041310	36.471	85	50	2	57	140	79	160	4.57e-13	63.9
MS.gene93488.t1	MTR_4g110100	44.615	65	35	1	77	140	341	405	4.91e-13	65.1
MS.gene93488.t1	MTR_2g097620	33.846	65	43	0	77	141	171	235	5.02e-13	65.1
MS.gene93488.t1	MTR_5g041330	41.176	68	37	1	73	140	86	150	9.23e-13	63.2
MS.gene93488.t1	MTR_1g051105	34.667	75	49	0	66	140	130	204	5.34e-12	61.6
MS.gene93488.t1	MTR_4g108830	34.259	108	56	2	47	139	112	219	9.06e-12	60.5
MS.gene93488.t1	MTR_5g088720	34.667	75	49	0	66	140	217	291	9.50e-12	61.6
MS.gene93488.t1	MTR_5g088720	34.667	75	49	0	66	140	176	250	1.15e-11	61.2
MS.gene93488.t1	MTR_5g088720	34.667	75	49	0	66	140	171	245	1.20e-11	61.2
MS.gene93488.t1	MTR_2g007340	44.828	58	32	0	79	136	185	242	1.24e-11	60.8
MS.gene93488.t1	MTR_3g083480	32.877	73	44	1	67	139	214	281	1.35e-11	60.8
MS.gene93488.t1	MTR_6g016350	45.833	48	26	0	93	140	309	356	3.67e-11	59.7
MS.gene93488.t1	MTR_4g108830	36.364	77	48	1	64	139	115	191	4.18e-11	58.2
MS.gene93488.t1	MTR_8g056930	38.667	75	42	2	68	139	90	163	4.55e-11	57.8
MS.gene93488.t1	MTR_0186s0090	51.020	49	21	1	94	139	115	163	5.91e-11	57.4
MS.gene93488.t1	MTR_4g064570	37.500	64	40	0	78	141	164	227	9.92e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93488.t1	AT1G14200	44.444	63	35	0	78	140	92	154	4.10e-16	71.2
MS.gene93488.t1	AT3G02340	29.371	143	83	5	1	140	253	380	2.35e-14	68.9
MS.gene93488.t1	AT3G02340	29.371	143	83	5	1	140	253	380	2.35e-14	68.9
MS.gene93488.t1	AT1G60360	40.625	64	37	1	78	140	206	269	5.23e-14	67.8
MS.gene93488.t1	AT5G08139	42.188	64	36	1	78	140	291	354	6.24e-14	67.8
MS.gene93488.t1	AT5G01980	40.625	64	38	0	77	140	332	395	8.16e-14	67.8
MS.gene93488.t1	AT3G60080	39.286	56	34	0	85	140	159	214	2.25e-13	65.9
MS.gene93488.t1	AT5G60820	45.283	53	29	0	89	141	366	418	3.01e-13	65.9
MS.gene93488.t1	AT4G05350	43.939	66	32	2	79	139	139	204	6.83e-13	63.5
MS.gene93488.t1	AT5G15820	37.662	77	47	1	65	140	260	336	8.57e-13	64.3
MS.gene93488.t1	AT4G12190	47.619	63	28	2	79	136	4	66	9.82e-13	59.7
MS.gene93488.t1	AT2G40830	36.709	79	49	1	64	141	158	236	1.21e-12	63.9
MS.gene93488.t1	AT2G40830	36.709	79	49	1	64	141	158	236	1.21e-12	63.9
MS.gene93488.t1	AT2G40830	36.709	79	49	1	64	141	158	236	1.21e-12	63.9
MS.gene93488.t1	AT2G40830	36.709	79	49	1	64	141	158	236	1.21e-12	63.9
MS.gene93488.t1	AT2G44330	52.174	46	22	0	95	140	96	141	1.69e-12	62.0
MS.gene93488.t1	AT3G10815	38.095	63	39	0	78	140	104	166	2.61e-12	61.6
MS.gene93488.t1	AT4G26400	41.270	63	32	1	78	140	229	286	3.12e-12	62.8
MS.gene93488.t1	AT4G26400	41.270	63	32	1	78	140	229	286	3.12e-12	62.8
MS.gene93488.t1	AT4G12150	50.909	55	25	1	82	134	140	194	5.15e-12	62.4
MS.gene93488.t1	AT4G12210	46.032	63	29	2	79	136	135	197	8.10e-12	60.5
MS.gene93488.t1	AT4G12140	46.774	62	29	2	79	136	135	196	1.06e-11	60.1
MS.gene93488.t1	AT3G30460	28.571	133	76	4	13	139	22	141	1.74e-11	58.5
MS.gene93488.t1	AT3G19950	36.508	63	39	1	79	140	199	261	2.14e-11	60.5
MS.gene93488.t1	AT3G19950	36.508	63	39	1	79	140	257	319	2.19e-11	60.5
MS.gene93488.t1	AT3G19950	36.508	63	39	1	79	140	257	319	2.19e-11	60.5
MS.gene93488.t1	AT3G30460	28.571	133	76	4	13	139	58	177	2.40e-11	58.9
MS.gene93488.t1	AT5G54990	51.111	45	22	0	92	136	171	215	3.86e-11	58.9
MS.gene93488.t1	AT1G55530	29.730	74	47	1	67	140	167	235	6.15e-11	58.9
MS.gene93488.t1	AT1G55530	30.137	73	46	1	68	140	201	268	6.46e-11	59.3
MS.gene93488.t1	AT1G55530	30.137	73	46	1	68	140	201	268	6.46e-11	59.3
MS.gene93488.t1	AT5G64920	37.931	58	36	0	79	136	241	298	7.29e-11	58.9
MS.gene93488.t1	AT1G21960	43.137	51	29	0	90	140	153	203	8.75e-11	57.8
MS.gene93488.t1	AT3G56580	34.211	76	49	1	66	140	156	231	9.06e-11	58.5
MS.gene93488.t1	AT3G56580	34.211	76	49	1	66	140	156	231	9.06e-11	58.5
MS.gene93488.t1	AT3G56580	34.211	76	49	1	66	140	156	231	9.06e-11	58.5
MS.gene93488.t1	AT3G14320	36.250	80	50	1	61	139	54	133	9.59e-11	57.8

Find 23 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAATCACTTTCTACATTTAT+AGG	0.123833	3.4:-17883072	None:intergenic
TATCTGTTTGGTGGAGCTTT+TGG	0.279649	3.4:+17883329	MS.gene93488:CDS
CATAAACAAACTCAGATATT+AGG	0.326615	3.4:-17883459	None:intergenic
GATTACGATTAGAATGCCTT+TGG	0.413240	3.4:-17883254	None:intergenic
ACGAAGGATGTATTATGAAA+TGG	0.423422	3.4:+17883394	MS.gene93488:CDS
GAACCTCGTTGATGAGGTTA+TGG	0.437573	3.4:-17883203	None:intergenic
TTATGGTGGCGAACAATAGA+AGG	0.439093	3.4:-17883186	None:intergenic
AATGATGTGTTCTATCTGTT+TGG	0.461955	3.4:+17883317	MS.gene93488:CDS
GATGTGTTCTATCTGTTTGG+TGG	0.496529	3.4:+17883320	MS.gene93488:CDS
AATTAGAGTTGAAGAAGGAA+AGG	0.503387	3.4:+17883284	MS.gene93488:CDS
ATAACCTCATCAACGAGGTT+CGG	0.516089	3.4:+17883205	MS.gene93488:CDS
GGAACAGTCTGACGACACAT+AGG	0.570479	3.4:-17883438	None:intergenic
GAACAGTCTGACGACACATA+GGG	0.586165	3.4:-17883437	None:intergenic
GGATGTATTATGAAATGGCT+AGG	0.587358	3.4:+17883399	MS.gene93488:CDS
TGGTTGGATCGCAAGCAACT+CGG	0.597083	3.4:+17883349	MS.gene93488:CDS
CCTCGTTGATGAGGTTATGG+TGG	0.604309	3.4:-17883200	None:intergenic
TAATCCGAACCTCGTTGATG+AGG	0.608297	3.4:-17883209	None:intergenic
TCGTGATACAAATGTGAACA+TGG	0.636330	3.4:-17883375	None:intergenic
GAAATACTGAAACTATCCAA+AGG	0.644867	3.4:+17883238	MS.gene93488:CDS
TGTTCACATTTGTATCACGA+AGG	0.673438	3.4:+17883378	MS.gene93488:CDS
GCTCATCGTCGTCATACACA+TGG	0.711088	3.4:+17883129	MS.gene93488:CDS
ATGGTACAAATCGATCAACA+AGG	0.728585	3.4:+17883148	MS.gene93488:CDS
CCACCATAACCTCATCAACG+AGG	0.765219	3.4:+17883200	MS.gene93488:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATCACTTTCTACATTTAT+AGG	-	chr3.4:17883075-17883094	None:intergenic	20.0%
	AATGATGTGTTCTATCTGTT+TGG	+	chr3.4:17883317-17883336	MS.gene93488:CDS	30.0%
	AATTAGAGTTGAAGAAGGAA+AGG	+	chr3.4:17883284-17883303	MS.gene93488:CDS	30.0%
	ACGAAGGATGTATTATGAAA+TGG	+	chr3.4:17883394-17883413	MS.gene93488:CDS	30.0%
	CCAAAAATTAGAGTTGAAGA+AGG	+	chr3.4:17883279-17883298	MS.gene93488:CDS	30.0%
	GAAATACTGAAACTATCCAA+AGG	+	chr3.4:17883238-17883257	MS.gene93488:CDS	30.0%
!	CCTTCTTCAACTCTAATTTT+TGG	-	chr3.4:17883282-17883301	None:intergenic	30.0%
	ATGGTACAAATCGATCAACA+AGG	+	chr3.4:17883148-17883167	MS.gene93488:CDS	35.0%
	GATTACGATTAGAATGCCTT+TGG	-	chr3.4:17883257-17883276	None:intergenic	35.0%
	GGATGTATTATGAAATGGCT+AGG	+	chr3.4:17883399-17883418	MS.gene93488:CDS	35.0%
	TCGTGATACAAATGTGAACA+TGG	-	chr3.4:17883378-17883397	None:intergenic	35.0%
	TGTTCACATTTGTATCACGA+AGG	+	chr3.4:17883378-17883397	MS.gene93488:CDS	35.0%
	ATAACCTCATCAACGAGGTT+CGG	+	chr3.4:17883205-17883224	MS.gene93488:CDS	40.0%
	GATGTGTTCTATCTGTTTGG+TGG	+	chr3.4:17883320-17883339	MS.gene93488:CDS	40.0%
!	TTATGGTGGCGAACAATAGA+AGG	-	chr3.4:17883189-17883208	None:intergenic	40.0%
!!	TATCTGTTTGGTGGAGCTTT+TGG	+	chr3.4:17883329-17883348	MS.gene93488:CDS	40.0%
	GAACAGTCTGACGACACATA+GGG	-	chr3.4:17883440-17883459	None:intergenic	45.0%
	TAATCCGAACCTCGTTGATG+AGG	-	chr3.4:17883212-17883231	None:intergenic	45.0%
!	GAACCTCGTTGATGAGGTTA+TGG	-	chr3.4:17883206-17883225	None:intergenic	45.0%
!!	TGTTTGGTGGAGCTTTTGGT+TGG	+	chr3.4:17883333-17883352	MS.gene93488:CDS	45.0%
	CCACCATAACCTCATCAACG+AGG	+	chr3.4:17883200-17883219	MS.gene93488:CDS	50.0%
	GCTCATCGTCGTCATACACA+TGG	+	chr3.4:17883129-17883148	MS.gene93488:CDS	50.0%
	GGAACAGTCTGACGACACAT+AGG	-	chr3.4:17883441-17883460	None:intergenic	50.0%
	TGGTTGGATCGCAAGCAACT+CGG	+	chr3.4:17883349-17883368	MS.gene93488:CDS	50.0%
!	CCTCGTTGATGAGGTTATGG+TGG	-	chr3.4:17883203-17883222	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	17883042	17883470	17883042	ID=MS.gene93488
chr3.4	mRNA	17883042	17883470	17883042	ID=MS.gene93488.t1;Parent=MS.gene93488
chr3.4	exon	17883042	17883470	17883042	ID=MS.gene93488.t1.exon1;Parent=MS.gene93488.t1
chr3.4	CDS	17883042	17883470	17883042	ID=cds.MS.gene93488.t1;Parent=MS.gene93488.t1

Gene Sequence

>MS.gene93488

ATGGAGCAACAACAACGTAATATAAGAAGTCCTATAAATGTAGAAAGTGATTATGTAATGATTCTTGATGCTGTTGATCTAAATCTCGCTCATCGTCGTCATACACATGGTACAAATCGATCAACAAGGTTCACTCTCTTGCGTCCTTCTATTGTTCGCCACCATAACCTCATCAACGAGGTTCGGATTACTATAAGAAATACTGAAACTATCCAAAGGCATTCTAATCGTAATCTTCCAAAAATTAGAGTTGAAGAAGGAAAGGAGTCTGAAGCAATGATGTGTTCTATCTGTTTGGTGGAGCTTTTGGTTGGATCGCAAGCAACTCGGTTGCCATGTTCACATTTGTATCACGAAGGATGTATTATGAAATGGCTAGGTAGATCAAACACTTGCCCTATGTGTCGTCAGACTGTTCCTAATATCTGA

Protein sequence

>MS.gene93488.t1

MEQQQRNIRSPINVESDYVMILDAVDLNLAHRRHTHGTNRSTRFTLLRPSIVRHHNLINEVRITIRNTETIQRHSNRNLPKIRVEEGKESEAMMCSICLVELLVGSQATRLPCSHLYHEGCIMKWLGRSNTCPMCRQTVPNI