AlfalfaGEDB | MS.gene94532

M. sativa cultivar XinJiangDaYe / MS.gene94532

KEGG: -
GO: GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94532.t1	AES69216.1	85.7	154	22	0	1	154	1	154	1.70E-72	282

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94532.t1	Q6AVN2	37.3	83	44	2	67	146	163	240	4.2e-11	69.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94532.t1	G7IZ80	85.7	154	22	0	1	154	1	154	1.2e-72	282.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene79416	MS.gene94532	PPI
MS.gene047623	MS.gene94532	PPI
MS.gene94532	MS.gene97792	PPI
MS.gene61922	MS.gene94532	PPI
MS.gene94532	MS.gene017995	PPI
MS.gene94532	MS.gene041075	PPI
MS.gene94532	MS.gene28214	PPI
MS.gene43658	MS.gene94532	PPI
MS.gene94532	MS.gene010008	PPI
MS.gene28213	MS.gene94532	PPI
MS.gene94532	MS.gene93488	PPI
MS.gene94532	MS.gene94534	PPI
MS.gene94532	MS.gene94527	PPI
MS.gene93487	MS.gene94532	PPI
MS.gene47429	MS.gene94532	PPI
MS.gene94532	MS.gene017970	PPI
MS.gene94532	MS.gene055988	PPI
MS.gene94532	MS.gene94530	PPI
MS.gene94532	MS.gene79417	PPI
MS.gene010343	MS.gene94532	PPI
MS.gene029745	MS.gene94532	PPI
MS.gene94532	MS.gene61924	PPI
MS.gene61921	MS.gene94532	PPI
MS.gene065401	MS.gene94532	PPI
MS.gene017995	MS.gene94532	PPI
MS.gene94532	MS.gene023766	PPI
MS.gene93489	MS.gene94532	PPI
MS.gene015160	MS.gene94532	PPI
MS.gene94532	MS.gene010006	PPI
MS.gene94532	MS.gene28215	PPI
MS.gene94532	MS.gene94537	PPI
MS.gene94532	MS.gene023764	PPI
MS.gene94532	MS.gene94533	PPI
MS.gene94532	MS.gene52667	PPI
MS.gene94532	MS.gene051416	PPI
MS.gene28215	MS.gene94532	PPI
MS.gene065402	MS.gene94532	PPI
MS.gene43652	MS.gene94532	PPI
MS.gene61920	MS.gene94532	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94532.t1	MTR_3g025510	85.714	154	22	0	1	154	1	154	8.16e-98	278
MS.gene94532.t1	MTR_3g025380	45.517	145	70	2	5	149	3	138	3.19e-30	107
MS.gene94532.t1	MTR_3g025550	72.222	54	15	0	94	147	6	59	8.94e-24	88.2
MS.gene94532.t1	MTR_3g025620	67.308	52	17	0	98	149	1	52	4.21e-21	81.3
MS.gene94532.t1	MTR_2g058360	50.633	79	33	3	75	149	64	140	2.90e-18	76.3
MS.gene94532.t1	MTR_7g074170	40.860	93	51	2	58	146	294	386	1.71e-17	78.6
MS.gene94532.t1	MTR_6g079660	36.471	85	50	2	66	146	192	276	1.07e-15	73.2
MS.gene94532.t1	MTR_1g114320	31.034	87	56	2	67	149	152	238	6.36e-14	67.8
MS.gene94532.t1	MTR_1g114620	37.634	93	52	2	58	146	215	305	7.31e-14	67.8
MS.gene94532.t1	MTR_0067s0100	32.292	96	65	0	51	146	136	231	1.83e-13	66.2
MS.gene94532.t1	MTR_3g072630	40.000	85	50	1	66	149	181	265	2.70e-13	66.2
MS.gene94532.t1	MTR_3g072630	40.000	85	50	1	66	149	181	265	3.32e-13	65.9
MS.gene94532.t1	MTR_3g083480	33.721	86	49	2	67	149	201	281	6.29e-13	65.1
MS.gene94532.t1	MTR_3g088080	35.714	70	41	2	81	149	16	82	1.27e-12	60.5
MS.gene94532.t1	MTR_2g097620	34.328	67	44	0	83	149	167	233	1.57e-12	63.9
MS.gene94532.t1	MTR_4g108830	33.628	113	64	4	38	149	89	191	4.68e-12	61.2
MS.gene94532.t1	MTR_4g064570	41.176	68	38	2	83	149	159	225	4.77e-12	62.8
MS.gene94532.t1	MTR_4g108830	42.857	63	35	1	88	149	157	219	1.18e-11	60.5
MS.gene94532.t1	MTR_5g080350	40.984	61	36	0	89	149	177	237	2.87e-11	60.5
MS.gene94532.t1	MTR_5g041330	43.103	58	30	1	89	146	92	146	3.82e-11	59.3
MS.gene94532.t1	MTR_5g041310	43.103	58	30	1	89	146	102	156	4.03e-11	59.3
MS.gene94532.t1	MTR_3g081030	39.683	63	37	1	88	149	102	164	4.96e-11	58.9
MS.gene94532.t1	MTR_5g058530	46.939	49	26	0	98	146	217	265	5.10e-11	59.7
MS.gene94532.t1	MTR_0186s0090	44.898	49	24	1	105	150	116	164	6.37e-11	57.8
MS.gene94532.t1	MTR_2g007340	40.984	61	36	0	89	149	185	245	6.86e-11	59.3
MS.gene94532.t1	MTR_6g016350	46.809	47	25	0	103	149	309	355	7.64e-11	59.3
MS.gene94532.t1	MTR_1g051105	46.667	45	24	0	105	149	159	203	7.72e-11	58.9
MS.gene94532.t1	MTR_8g056930	44.898	49	24	1	105	150	116	164	9.97e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94532.t1	AT5G08139	46.154	65	34	1	83	146	286	350	2.06e-14	69.7
MS.gene94532.t1	AT1G14200	37.333	75	47	0	72	146	76	150	5.32e-14	66.2
MS.gene94532.t1	AT4G26400	33.654	104	55	3	51	146	185	282	6.86e-14	68.2
MS.gene94532.t1	AT4G26400	33.654	104	55	3	51	146	185	282	6.86e-14	68.2
MS.gene94532.t1	AT5G01980	34.737	95	59	1	58	149	300	394	7.21e-14	68.2
MS.gene94532.t1	AT4G05350	44.776	67	32	2	89	150	139	205	2.01e-13	65.1
MS.gene94532.t1	AT4G12190	43.284	67	33	2	89	150	4	70	5.81e-13	60.8
MS.gene94532.t1	AT4G12210	41.791	67	34	2	89	150	135	201	8.89e-13	63.5
MS.gene94532.t1	AT3G02340	40.230	87	47	2	64	149	297	379	1.08e-12	64.7
MS.gene94532.t1	AT3G02340	40.230	87	47	2	64	149	297	379	1.08e-12	64.7
MS.gene94532.t1	AT3G19950	32.143	84	53	2	67	146	232	315	1.42e-12	64.3
MS.gene94532.t1	AT3G19950	32.143	84	53	2	67	146	232	315	1.42e-12	64.3
MS.gene94532.t1	AT3G19950	32.143	84	53	2	67	146	174	257	1.49e-12	63.9
MS.gene94532.t1	AT3G30460	46.032	63	32	1	89	149	115	177	1.50e-12	62.4
MS.gene94532.t1	AT3G30460	46.032	63	32	1	89	149	79	141	1.53e-12	61.6
MS.gene94532.t1	AT5G15820	42.647	68	38	1	83	149	268	335	1.85e-12	63.9
MS.gene94532.t1	AT5G54990	53.333	45	21	0	102	146	171	215	2.73e-12	62.4
MS.gene94532.t1	AT2G40830	38.806	67	40	1	89	154	173	239	3.27e-12	63.2
MS.gene94532.t1	AT2G40830	38.806	67	40	1	89	154	173	239	3.27e-12	63.2
MS.gene94532.t1	AT2G40830	38.806	67	40	1	89	154	173	239	3.27e-12	63.2
MS.gene94532.t1	AT2G40830	38.806	67	40	1	89	154	173	239	3.27e-12	63.2
MS.gene94532.t1	AT1G60360	28.030	132	84	3	34	154	142	273	3.67e-12	63.2
MS.gene94532.t1	AT3G10815	48.889	45	23	0	105	149	121	165	7.50e-12	60.8
MS.gene94532.t1	AT3G60080	38.806	67	33	1	88	146	144	210	8.82e-12	62.0
MS.gene94532.t1	AT4G12140	41.935	62	34	1	91	150	139	200	1.66e-11	60.1
MS.gene94532.t1	AT1G18780	41.509	53	31	0	97	149	269	321	2.28e-11	60.8
MS.gene94532.t1	AT3G13430	30.952	84	50	2	67	146	187	266	3.35e-11	60.1
MS.gene94532.t1	AT3G13430	30.952	84	50	2	67	146	187	266	3.35e-11	60.1
MS.gene94532.t1	AT3G13430	30.952	84	50	2	67	146	187	266	3.35e-11	60.1
MS.gene94532.t1	AT3G13430	30.952	84	50	2	67	146	187	266	3.35e-11	60.1
MS.gene94532.t1	AT3G14320	40.541	74	42	2	78	149	60	133	4.32e-11	58.9
MS.gene94532.t1	AT2G44330	48.000	50	26	0	105	154	96	145	4.48e-11	58.5
MS.gene94532.t1	AT5G60820	50.000	46	23	0	101	146	368	413	4.90e-11	60.1
MS.gene94532.t1	AT5G56340	33.735	83	47	2	67	146	223	300	5.04e-11	60.1
MS.gene94532.t1	AT1G18760	35.616	73	46	1	74	146	145	216	7.02e-11	58.5
MS.gene94532.t1	AT1G55530	31.325	83	49	2	67	146	154	231	8.32e-11	59.3
MS.gene94532.t1	AT1G55530	31.325	83	49	2	67	146	187	264	9.39e-11	58.9
MS.gene94532.t1	AT1G55530	31.325	83	49	2	67	146	187	264	9.39e-11	58.9

Find 23 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTCTTCAATTTCGACTCT+TGG	0.354455	3.2:-15547753	None:intergenic
TATTTGTTTAGCGGAGCTTC+CGG	0.405154	3.2:+15547803	MS.gene94532:CDS
CGAATTGCTTTCGACCCAAC+CGG	0.412863	3.2:-15547822	None:intergenic
CGGTTGGGTCGAAAGCAATT+CGG	0.413830	3.2:+15547823	MS.gene94532:CDS
GACGTGTTCTATTTGTTTAG+CGG	0.436455	3.2:+15547794	MS.gene94532:CDS
TGTTTAGCGGAGCTTCCGGT+TGG	0.459068	3.2:+15547807	MS.gene94532:CDS
CTTGTAAGAATGTGATAAAG+AGG	0.466886	3.2:-15547579	None:intergenic
ACGATGAGTGTATCATGAAA+TGG	0.467296	3.2:+15547868	MS.gene94532:CDS
TGATAACATCGAAAATATAG+TGG	0.486505	3.2:-15547668	None:intergenic
TTACAAGAAATTCATCAAGT+AGG	0.490702	3.2:+15547595	MS.gene94532:CDS
ATATAGTGGTCATCAACATA+AGG	0.511658	3.2:-15547654	None:intergenic
GTTTAGCGGAGCTTCCGGTT+GGG	0.513755	3.2:+15547808	MS.gene94532:CDS
AGAATGTGATAAAGAGGAGG+AGG	0.516829	3.2:-15547573	None:intergenic
AATCATGCTAGCAGAAGAAG+TGG	0.524626	3.2:+15547705	MS.gene94532:CDS
CCAAGAGTCGAAATTGAAGA+AGG	0.529637	3.2:+15547753	MS.gene94532:CDS
AATATGCTCTGACGACACAT+AGG	0.557782	3.2:-15547912	None:intergenic
ATATGCTCTGACGACACATA+GGG	0.605674	3.2:-15547911	None:intergenic
GTAAGAATGTGATAAAGAGG+AGG	0.610401	3.2:-15547576	None:intergenic
TAGCATGATTCTCGTTGATG+TGG	0.623325	3.2:-15547692	None:intergenic
TCGTGGTAGATATGCGAGCA+CGG	0.629183	3.2:-15547849	None:intergenic
GTCTAGTGAAGACAACGACT+CGG	0.664059	3.2:+15547521	MS.gene94532:CDS
CAATGGAGCAACATCAACGT+CGG	0.712043	3.2:+15547484	None:intergenic
ATTTCATGATACACTCATCG+TGG	0.739086	3.2:-15547866	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGATAACATCGAAAATATAG+TGG	-	chr3.2:15547671-15547690	None:intergenic	25.0%
!	TTACAAGAAATTCATCAAGT+AGG	+	chr3.2:15547595-15547614	MS.gene94532:CDS	25.0%
	ATATAGTGGTCATCAACATA+AGG	-	chr3.2:15547657-15547676	None:intergenic	30.0%
	CTTGTAAGAATGTGATAAAG+AGG	-	chr3.2:15547582-15547601	None:intergenic	30.0%
	ACGATGAGTGTATCATGAAA+TGG	+	chr3.2:15547868-15547887	MS.gene94532:CDS	35.0%
	ATTTCATGATACACTCATCG+TGG	-	chr3.2:15547869-15547888	None:intergenic	35.0%
	GACGTGTTCTATTTGTTTAG+CGG	+	chr3.2:15547794-15547813	MS.gene94532:CDS	35.0%
	GTAAGAATGTGATAAAGAGG+AGG	-	chr3.2:15547579-15547598	None:intergenic	35.0%
	AATATGCTCTGACGACACAT+AGG	-	chr3.2:15547915-15547934	None:intergenic	40.0%
	AATCATGCTAGCAGAAGAAG+TGG	+	chr3.2:15547705-15547724	MS.gene94532:CDS	40.0%
	AGAATGTGATAAAGAGGAGG+AGG	-	chr3.2:15547576-15547595	None:intergenic	40.0%
	ATATGCTCTGACGACACATA+GGG	-	chr3.2:15547914-15547933	None:intergenic	40.0%
	CCAAGAGTCGAAATTGAAGA+AGG	+	chr3.2:15547753-15547772	MS.gene94532:CDS	40.0%
	CCTTCTTCAATTTCGACTCT+TGG	-	chr3.2:15547756-15547775	None:intergenic	40.0%
	TAGCATGATTCTCGTTGATG+TGG	-	chr3.2:15547695-15547714	None:intergenic	40.0%
	TATTTGTTTAGCGGAGCTTC+CGG	+	chr3.2:15547803-15547822	MS.gene94532:CDS	40.0%
	GTCTAGTGAAGACAACGACT+CGG	+	chr3.2:15547521-15547540	MS.gene94532:CDS	45.0%
	CGAATTGCTTTCGACCCAAC+CGG	-	chr3.2:15547825-15547844	None:intergenic	50.0%
	CGGTTGGGTCGAAAGCAATT+CGG	+	chr3.2:15547823-15547842	MS.gene94532:CDS	50.0%
	TCGTGGTAGATATGCGAGCA+CGG	-	chr3.2:15547852-15547871	None:intergenic	50.0%
	GTTTAGCGGAGCTTCCGGTT+GGG	+	chr3.2:15547808-15547827	MS.gene94532:CDS	55.0%
	TGTTTAGCGGAGCTTCCGGT+TGG	+	chr3.2:15547807-15547826	MS.gene94532:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	15547486	15547950	15547486	ID=MS.gene94532
chr3.2	mRNA	15547486	15547950	15547486	ID=MS.gene94532.t1;Parent=MS.gene94532
chr3.2	exon	15547486	15547950	15547486	ID=MS.gene94532.t1.exon1;Parent=MS.gene94532.t1
chr3.2	CDS	15547486	15547950	15547486	ID=cds.MS.gene94532.t1;Parent=MS.gene94532.t1

Gene Sequence

>MS.gene94532

ATGGAGCAACATCAACGTCGGTCAAGAAGAAGTGTGTCTAGTGAAGACAACGACTCGGTTATGATTTTCGATGCTATTGATCTGAATCCTCCTCCTCTTTATCACATTCTTACAAGAAATTCATCAAGTAGGTTCGCAAAAAATTTCATAAGATCTACATCAACTACTCCTTATGTTGATGACCACTATATTTTCGATGTTATCAACCACATCAACGAGAATCATGCTAGCAGAAGAAGTGGAGTCAGACATGTTTATCGCAATCTTCCAAGAGTCGAAATTGAAGAAGGAATGAAGTGTGAAGCTTTGACGTGTTCTATTTGTTTAGCGGAGCTTCCGGTTGGGTCGAAAGCAATTCGGTTGCCGTGCTCGCATATCTACCACGATGAGTGTATCATGAAATGGTTAGATAGATCGAACACATGCCCTATGTGTCGTCAGAGCATATTTCATGTTTCTTCATGA

Protein sequence

>MS.gene94532.t1

MEQHQRRSRRSVSSEDNDSVMIFDAIDLNPPPLYHILTRNSSSRFAKNFIRSTSTTPYVDDHYIFDVINHINENHASRRSGVRHVYRNLPRVEIEEGMKCEALTCSICLAELPVGSKAIRLPCSHIYHDECIMKWLDRSNTCPMCRQSIFHVSS