AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024390.t1 MTR_7g118230 76.842 190 10 2 60 249 33 188 1.01e-88 266
MS.gene024390.t1 MTR_1g045510 36.364 176 100 3 70 244 113 277 2.01e-25 101
MS.gene024390.t1 MTR_1g056520 33.498 203 109 5 55 249 101 285 1.48e-24 99.8
MS.gene024390.t1 MTR_7g106440 30.688 189 127 3 57 242 96 283 1.25e-21 92.0
MS.gene024390.t1 MTR_3g027140 31.677 161 107 2 71 231 61 218 2.85e-20 86.7
MS.gene024390.t1 MTR_4g104470 30.387 181 117 4 71 250 123 295 1.08e-16 78.2
MS.gene024390.t1 MTR_4g104470 41.573 89 50 1 60 148 208 294 8.41e-14 70.1
MS.gene024390.t1 MTR_1g097440 34.161 161 91 4 62 222 113 258 2.32e-16 78.6
MS.gene024390.t1 MTR_3g075500 31.638 177 111 4 71 244 110 279 2.43e-16 77.0
MS.gene024390.t1 MTR_3g075500 37.778 90 52 2 55 143 193 279 6.03e-12 64.7
MS.gene024390.t1 MTR_1g064230 28.571 266 143 7 9 244 21 269 4.55e-16 76.3
MS.gene024390.t1 MTR_3g075500 29.762 168 109 3 71 236 110 270 1.21e-14 72.0
MS.gene024390.t1 MTR_2g097960 26.627 169 112 5 72 238 17 175 2.85e-12 66.2
MS.gene024390.t1 MTR_6g040190 31.126 151 88 5 72 222 115 249 6.10e-12 65.5
MS.gene024390.t1 MTR_7g118120 23.699 173 107 3 52 220 83 234 1.56e-11 63.9
MS.gene024390.t1 MTR_5g030020 30.682 176 113 5 72 245 98 266 2.58e-11 63.2
MS.gene024390.t1 MTR_3g099590 29.944 177 113 6 70 244 89 256 3.30e-11 62.4
MS.gene024390.t1 MTR_1g061870 28.977 176 120 5 70 244 194 365 3.52e-11 63.2
MS.gene024390.t1 MTR_1g115375 26.316 209 113 6 57 244 195 383 3.86e-11 62.8
MS.gene024390.t1 MTR_6g040070 29.801 151 93 3 72 222 17 154 4.52e-11 63.2
MS.gene024390.t1 MTR_4g085540 25.510 196 122 6 62 252 117 293 6.51e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024390.t1 AT1G01080 54.359 195 81 3 64 253 102 293 1.37e-68 213
MS.gene024390.t1 AT1G01080 54.082 196 81 4 64 253 102 294 7.10e-67 209
MS.gene024390.t1 AT1G01080 54.237 177 73 3 64 235 102 275 2.66e-59 189
MS.gene024390.t1 AT3G52150 29.646 226 139 6 37 247 33 253 1.37e-21 90.9
MS.gene024390.t1 AT3G52150 29.646 226 139 6 37 247 33 253 1.37e-21 90.9
MS.gene024390.t1 AT1G60000 30.741 270 141 11 1 244 1 250 3.90e-21 89.7
MS.gene024390.t1 AT3G52380 31.285 179 119 4 67 242 113 290 7.04e-21 90.1
MS.gene024390.t1 AT5G50250 30.570 193 119 5 59 250 108 286 9.60e-18 80.9
MS.gene024390.t1 AT5G50250 38.462 91 54 1 58 148 197 285 6.81e-13 67.4
MS.gene024390.t1 AT2G37220 28.230 209 116 4 61 246 82 279 1.14e-17 80.9
MS.gene024390.t1 AT4G24770 32.571 175 109 4 71 244 151 317 2.56e-15 74.7
MS.gene024390.t1 AT4G24770 41.026 78 46 0 71 148 245 322 8.78e-13 67.4
MS.gene024390.t1 AT4G24770 32.571 175 109 4 71 244 151 317 2.56e-15 74.7
MS.gene024390.t1 AT4G24770 41.026 78 46 0 71 148 245 322 8.78e-13 67.4
MS.gene024390.t1 AT2G35410 31.937 191 121 6 57 245 86 269 3.35e-14 71.2
MS.gene024390.t1 AT3G16380 29.670 182 114 4 72 252 23 191 1.63e-13 70.1
MS.gene024390.t1 AT1G22760 28.342 187 121 4 34 220 103 276 5.12e-13 68.9
MS.gene024390.t1 AT1G71770 28.342 187 121 4 36 222 115 288 4.98e-12 65.9
MS.gene024390.t1 AT1G71770 28.342 187 121 4 36 222 115 288 4.98e-12 65.9
MS.gene024390.t1 AT2G23350 25.668 187 116 5 72 252 136 305 1.49e-11 64.3
MS.gene024390.t1 AT1G34140 32.215 149 86 5 72 220 33 166 5.39e-11 62.4
MS.gene024390.t1 AT4G34110 26.882 186 115 6 72 252 126 295 6.67e-11 62.4

Find 60 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACATCTCTTTGGTAAATT+TGG 0.099306 7.2:-385288 MS.gene024390:CDS
AAGAGATGTCTCACGTCTTC+AGG 0.315903 7.2:+385300 None:intergenic
TGTTGAGTATCACAGCTTCT+AGG 0.370545 7.2:+385765 None:intergenic
TCTCACCAGTCTCTGCATTT+CGG 0.377067 7.2:+385625 None:intergenic
CAGAGACTGGTGAGAGTAAA+GGG 0.393341 7.2:-385617 MS.gene024390:CDS
CTCCAAGTAGTGGCTGCTTA+TGG 0.394918 7.2:+385937 None:intergenic
TACCATAAGCAGCCACTACT+TGG 0.428423 7.2:-385939 MS.gene024390:CDS
GGTGTTGCAAGATTTCAATC+AGG 0.443133 7.2:-385247 MS.gene024390:CDS
TAACGAAGAGGAAGAGAAAG+AGG 0.445652 7.2:+385965 None:intergenic
TTCCCAACATACACCTTATG+AGG 0.456223 7.2:+385342 None:intergenic
GAAGGTTGCAGACGTAAACT+TGG 0.462333 7.2:+385787 None:intergenic
TGTTGGTGGGCGCGAAATGC+GGG 0.472414 7.2:-385445 MS.gene024390:CDS
CTTTGGTAAATTTGGAAATG+TGG 0.479793 7.2:-385280 MS.gene024390:CDS
GCAGAGACTGGTGAGAGTAA+AGG 0.480321 7.2:-385618 MS.gene024390:CDS
GTCCTCATAAGGTGTATGTT+GGG 0.485859 7.2:-385344 MS.gene024390:CDS
ATACTATGAAGGTCCTCATA+AGG 0.498833 7.2:-385355 MS.gene024390:CDS
TTGCAGACGTAAACTTGGGT+TGG 0.499589 7.2:+385792 None:intergenic
GAAGACGTGAGACATCTCTT+TGG 0.500754 7.2:-385297 MS.gene024390:CDS
GGGAGTGCTTATGTCACAAT+GGG 0.508546 7.2:-385597 MS.gene024390:CDS
TCGTTTGAATTGAAGGATGT+TGG 0.510713 7.2:-385462 MS.gene024390:intron
ACAACCACGTAAGTTGAAGC+TGG 0.512125 7.2:-385820 MS.gene024390:CDS
CGCGAGGAGAAAGAAGGATA+AGG 0.512495 7.2:+385876 None:intergenic
AATGCACTTGCTGCATTGGA+TGG 0.517474 7.2:-385555 MS.gene024390:CDS
GGTCCTCATAAGGTGTATGT+TGG 0.523656 7.2:-385345 MS.gene024390:CDS
GAAACACAGAGTAGCAGAGT+TGG 0.527590 7.2:+385989 None:intergenic
GTATATAGTTCTTCTTCTCA+CGG 0.533646 7.2:+385374 None:intergenic
TCTAGTGGTTAGAGAGGGTG+TGG 0.535491 7.2:-385050 MS.gene024390:CDS
AGAAGAACTATATACTATGA+AGG 0.536527 7.2:-385366 MS.gene024390:CDS
ATGTTGGTGGGCGCGAAATG+CGG 0.539679 7.2:-385446 MS.gene024390:CDS
TATATAGTTCTTCTTCTCAC+GGG 0.541659 7.2:+385375 None:intergenic
TCGAACTCTAGTGGTTAGAG+AGG 0.547494 7.2:-385056 MS.gene024390:CDS
CAACCACGTAAGTTGAAGCT+GGG 0.548485 7.2:-385819 MS.gene024390:CDS
AAGCAGCCACTACTTGGAGT+TGG 0.552480 7.2:-385933 MS.gene024390:CDS
GAGGAGTGAAGAGATGAAGT+AGG 0.557135 7.2:+385742 None:intergenic
CTGTGACGCGAGGAGAAAGA+AGG 0.561006 7.2:+385870 None:intergenic
GTGTTGCAAGATTTCAATCA+GGG 0.561480 7.2:-385246 MS.gene024390:CDS
TTTGAATTGAAGGATGTTGG+TGG 0.563904 7.2:-385459 MS.gene024390:intron
GTTTGCCGAAATGCAGAGAC+TGG 0.571702 7.2:-385630 MS.gene024390:CDS
TTGAATTGAAGGATGTTGGT+GGG 0.579799 7.2:-385458 MS.gene024390:intron
GGTTTCATTGAGGGACAAAG+AGG 0.584074 7.2:+385167 None:intergenic
GAGTATCCAACTCCAAGTAG+TGG 0.587862 7.2:+385927 None:intergenic
GAGTATCACAGCTTCTAGGA+AGG 0.590293 7.2:+385769 None:intergenic
CTTCATCTCTTCACTCCTCA+CGG 0.594839 7.2:-385738 MS.gene024390:CDS
TTCCCCAGCTTCAACTTACG+TGG 0.601446 7.2:+385816 None:intergenic
AGGGAGTGCTTATGTCACAA+TGG 0.612091 7.2:-385598 MS.gene024390:CDS
AAGGTTGCAGACGTAAACTT+GGG 0.613456 7.2:+385788 None:intergenic
TTGAGAGAGATAAGAGACAA+AGG 0.615725 7.2:+385203 None:intergenic
AATGGACGCGCTGTGACGCG+AGG 0.621619 7.2:+385860 None:intergenic
ACTTGCTGCATTGGATGGAT+CGG 0.623524 7.2:-385550 MS.gene024390:intron
TGGAAATGTGGCTAGTGTGA+GGG 0.626364 7.2:-385268 MS.gene024390:CDS
ATAGTTCTTCTTCTCACGGG+AGG 0.629773 7.2:+385378 None:intergenic
TTGGAAATGTGGCTAGTGTG+AGG 0.645229 7.2:-385269 MS.gene024390:CDS
ACGTACCTCGGTAGATAGAA+CGG 0.653839 7.2:+385713 None:intergenic
CGAACTCTAGTGGTTAGAGA+GGG 0.654487 7.2:-385055 MS.gene024390:CDS
AACCACGTAAGTTGAAGCTG+GGG 0.663989 7.2:-385818 MS.gene024390:CDS
TGTTGCAAGATTTCAATCAG+GGG 0.681995 7.2:-385245 MS.gene024390:CDS
CTTATGGTAGAGTAACGAAG+AGG 0.714741 7.2:+385953 None:intergenic
AAGAGACAAAGGCATAGACA+CGG 0.730141 7.2:+385214 None:intergenic
GTAGATAGAACGGTACCGTG+AGG 0.731002 7.2:+385723 None:intergenic
CGGTACCGTTCTATCTACCG+AGG 0.742490 7.2:-385718 MS.gene024390:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAGAACTATATACTATGA+AGG - chr7.2:385663-385682 MS.gene024390:intron 25.0%
!! ATATTTTACGGTTTCATTGA+GGG + chr7.2:385874-385893 None:intergenic 25.0%
!!! TTGTTTTTCGTTTGAATTGA+AGG - chr7.2:385560-385579 MS.gene024390:CDS 25.0%
AGACATCTCTTTGGTAAATT+TGG - chr7.2:385741-385760 MS.gene024390:CDS 30.0%
CTTTGGTAAATTTGGAAATG+TGG - chr7.2:385749-385768 MS.gene024390:CDS 30.0%
GCAGTAGAAAAAGTGAAAAA+AGG + chr7.2:385904-385923 None:intergenic 30.0%
GTAGAAAAAGTGAAAAAAGG+TGG + chr7.2:385901-385920 None:intergenic 30.0%
GTATATAGTTCTTCTTCTCA+CGG + chr7.2:385658-385677 None:intergenic 30.0%
TATATAGTTCTTCTTCTCAC+GGG + chr7.2:385657-385676 None:intergenic 30.0%
! CATATTTTACGGTTTCATTG+AGG + chr7.2:385875-385894 None:intergenic 30.0%
! TTCTTCTTCGTTTTCTACAA+TGG + chr7.2:385190-385209 None:intergenic 30.0%
ATACTATGAAGGTCCTCATA+AGG - chr7.2:385674-385693 MS.gene024390:intron 35.0%
GAAAAAGTGAAAAAAGGTGG+AGG + chr7.2:385898-385917 None:intergenic 35.0%
GTGTTGCAAGATTTCAATCA+GGG - chr7.2:385783-385802 MS.gene024390:CDS 35.0%
TCGTTTGAATTGAAGGATGT+TGG - chr7.2:385567-385586 MS.gene024390:CDS 35.0%
TGTTGCAAGATTTCAATCAG+GGG - chr7.2:385784-385803 MS.gene024390:CDS 35.0%
TTGAATTGAAGGATGTTGGT+GGG - chr7.2:385571-385590 MS.gene024390:CDS 35.0%
TTGAGAGAGATAAGAGACAA+AGG + chr7.2:385829-385848 None:intergenic 35.0%
TTTGAATTGAAGGATGTTGG+TGG - chr7.2:385570-385589 MS.gene024390:CDS 35.0%
! TAAAAATGCACTTGCTGCAT+TGG - chr7.2:385470-385489 MS.gene024390:intron 35.0%
! TTGTTCTTGCAGCAATTTTG+TGG - chr7.2:385950-385969 MS.gene024390:CDS 35.0%
!! AAAGGTGGAGGCATATTTTA+CGG + chr7.2:385886-385905 None:intergenic 35.0%
AAGGTTGCAGACGTAAACTT+GGG + chr7.2:385244-385263 None:intergenic 40.0%
AGCAACATACTTACGTACCT+CGG + chr7.2:385331-385350 None:intergenic 40.0%
CTTATGGTAGAGTAACGAAG+AGG + chr7.2:385079-385098 None:intergenic 40.0%
GTAATGTAATGCTGTGCTGT+AGG - chr7.2:385377-385396 MS.gene024390:CDS 40.0%
GTCCTCATAAGGTGTATGTT+GGG - chr7.2:385685-385704 MS.gene024390:intron 40.0%
TAACGAAGAGGAAGAGAAAG+AGG + chr7.2:385067-385086 None:intergenic 40.0%
TGTTGAGTATCACAGCTTCT+AGG + chr7.2:385267-385286 None:intergenic 40.0%
TTCCCAACATACACCTTATG+AGG + chr7.2:385690-385709 None:intergenic 40.0%
! AAGAGACAAAGGCATAGACA+CGG + chr7.2:385818-385837 None:intergenic 40.0%
! ATTTTGTGGTCGAACTCTAG+TGG - chr7.2:385964-385983 MS.gene024390:CDS 40.0%
! GGTGTTGCAAGATTTCAATC+AGG - chr7.2:385782-385801 MS.gene024390:CDS 40.0%
AAGAGATGTCTCACGTCTTC+AGG + chr7.2:385732-385751 None:intergenic 45.0%
ACGTACCTCGGTAGATAGAA+CGG + chr7.2:385319-385338 None:intergenic 45.0%
ATAGTTCTTCTTCTCACGGG+AGG + chr7.2:385654-385673 None:intergenic 45.0%
CAGAGACTGGTGAGAGTAAA+GGG - chr7.2:385412-385431 MS.gene024390:CDS 45.0%
CGAACTCTAGTGGTTAGAGA+GGG - chr7.2:385974-385993 MS.gene024390:CDS 45.0%
CTTCATCTCTTCACTCCTCA+CGG - chr7.2:385291-385310 MS.gene024390:CDS 45.0%
GAAACACAGAGTAGCAGAGT+TGG + chr7.2:385043-385062 None:intergenic 45.0%
GAAGACGTGAGACATCTCTT+TGG - chr7.2:385732-385751 MS.gene024390:CDS 45.0%
GAAGGTTGCAGACGTAAACT+TGG + chr7.2:385245-385264 None:intergenic 45.0%
GAGGAGTGAAGAGATGAAGT+AGG + chr7.2:385290-385309 None:intergenic 45.0%
GAGTATCCAACTCCAAGTAG+TGG + chr7.2:385105-385124 None:intergenic 45.0%
GCTGCAAGAACAAGACTAGT+AGG + chr7.2:385943-385962 None:intergenic 45.0%
GGTCCTCATAAGGTGTATGT+TGG - chr7.2:385684-385703 MS.gene024390:intron 45.0%
GGTTTCATTGAGGGACAAAG+AGG + chr7.2:385865-385884 None:intergenic 45.0%
TACCATAAGCAGCCACTACT+TGG - chr7.2:385090-385109 MS.gene024390:intron 45.0%
TCGAACTCTAGTGGTTAGAG+AGG - chr7.2:385973-385992 MS.gene024390:CDS 45.0%
TCTCACCAGTCTCTGCATTT+CGG + chr7.2:385407-385426 None:intergenic 45.0%
TGGAAATGTGGCTAGTGTGA+GGG - chr7.2:385761-385780 MS.gene024390:CDS 45.0%
TTGCAGACGTAAACTTGGGT+TGG + chr7.2:385240-385259 None:intergenic 45.0%
TTGGAAATGTGGCTAGTGTG+AGG - chr7.2:385760-385779 MS.gene024390:CDS 45.0%
! AACCACGTAAGTTGAAGCTG+GGG - chr7.2:385211-385230 MS.gene024390:CDS 45.0%
! AATGCACTTGCTGCATTGGA+TGG - chr7.2:385474-385493 MS.gene024390:intron 45.0%
! ACAACCACGTAAGTTGAAGC+TGG - chr7.2:385209-385228 MS.gene024390:CDS 45.0%
! ACTTGCTGCATTGGATGGAT+CGG - chr7.2:385479-385498 MS.gene024390:intron 45.0%
! CAACCACGTAAGTTGAAGCT+GGG - chr7.2:385210-385229 MS.gene024390:CDS 45.0%
! GAGTATCACAGCTTCTAGGA+AGG + chr7.2:385263-385282 None:intergenic 45.0%
!! AGGGAGTGCTTATGTCACAA+TGG - chr7.2:385431-385450 MS.gene024390:CDS 45.0%
!! GGGAGTGCTTATGTCACAAT+GGG - chr7.2:385432-385451 MS.gene024390:CDS 45.0%
AAGCAGCCACTACTTGGAGT+TGG - chr7.2:385096-385115 MS.gene024390:intron 50.0%
CGCGAGGAGAAAGAAGGATA+AGG + chr7.2:385156-385175 None:intergenic 50.0%
CTCCAAGTAGTGGCTGCTTA+TGG + chr7.2:385095-385114 None:intergenic 50.0%
GCAGAGACTGGTGAGAGTAA+AGG - chr7.2:385411-385430 MS.gene024390:CDS 50.0%
GTAGATAGAACGGTACCGTG+AGG + chr7.2:385309-385328 None:intergenic 50.0%
GTTTGCCGAAATGCAGAGAC+TGG - chr7.2:385399-385418 MS.gene024390:CDS 50.0%
TCTAGTGGTTAGAGAGGGTG+TGG - chr7.2:385979-385998 MS.gene024390:CDS 50.0%
TTCCCCAGCTTCAACTTACG+TGG + chr7.2:385216-385235 None:intergenic 50.0%
CGGTACCGTTCTATCTACCG+AGG - chr7.2:385311-385330 MS.gene024390:CDS 55.0%
CTGTGACGCGAGGAGAAAGA+AGG + chr7.2:385162-385181 None:intergenic 55.0%
! ATGTTGGTGGGCGCGAAATG+CGG - chr7.2:385583-385602 MS.gene024390:CDS 55.0%
! TGTTGGTGGGCGCGAAATGC+GGG - chr7.2:385584-385603 MS.gene024390:CDS 60.0%
AATGGACGCGCTGTGACGCG+AGG + chr7.2:385172-385191 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 385033 386018 385033 ID=MS.gene024390
chr7.2 mRNA 385033 386018 385033 ID=MS.gene024390.t1;Parent=MS.gene024390
chr7.2 exon 385719 386018 385719 ID=MS.gene024390.t1.exon1;Parent=MS.gene024390.t1
chr7.2 CDS 385719 386018 385719 ID=cds.MS.gene024390.t1;Parent=MS.gene024390.t1
chr7.2 exon 385551 385652 385551 ID=MS.gene024390.t1.exon2;Parent=MS.gene024390.t1
chr7.2 CDS 385551 385652 385551 ID=cds.MS.gene024390.t1;Parent=MS.gene024390.t1
chr7.2 exon 385167 385469 385167 ID=MS.gene024390.t1.exon3;Parent=MS.gene024390.t1
chr7.2 CDS 385167 385469 385167 ID=cds.MS.gene024390.t1;Parent=MS.gene024390.t1
chr7.2 exon 385033 385089 385033 ID=MS.gene024390.t1.exon4;Parent=MS.gene024390.t1
chr7.2 CDS 385033 385089 385033 ID=cds.MS.gene024390.t1;Parent=MS.gene024390.t1
Gene Sequence

>MS.gene024390

ATGGCTGCCAACTCTGCTACTCTGTGTTTCTCCTCTTTCTCTTCCTCTTCGTTACTCTACCATAAGCAGCCACTACTTGGAGTTGGATACTCAAAACGATTCGTTTCGTTTTCTGCGCTGCCTTATCCTTCTTTCTCCTCGCGTCACAGCGCGTCCATTGTAGAAAACGAAGAAGAACAACCACGTAAGTTGAAGCTGGGGAATCCAACCCAAGTTTACGTCTGCAACCTTCCTAGAAGCTGTGATACTCAACACCTACTTCATCTCTTCACTCCTCACGGTACCGTTCTATCTACCGAGGTACGTAAGTATGTTGCTTTCTTCATTATTACTTGTGATGTAATGTAATGTAATGCTGTGCTGTAGGTTTGCCGAAATGCAGAGACTGGTGAGAGTAAAGGGAGTGCTTATGTCACAATGGGATCTTATAATGCTGCTAAAAATGCACTTGCTGCATTGGATGGATCGGTTAGTCACTATCATATCTAATAAATGCACTTGCTGCAATGCATCTGAATAATGAAACTTTGTTTTTCGTTTGAATTGAAGGATGTTGGTGGGCGCGAAATGCGGGTTAAATTTTCTGTTGAGATGAATAATTTAGAGACATTGAACTCACCTCCCGTGAGAAGAAGAACTATATACTATGAAGGTCCTCATAAGGTGTATGTTGGGAATCTCGCTAAGTCTGCTACACCTGAAGACGTGAGACATCTCTTTGGTAAATTTGGAAATGTGGCTAGTGTGAGGGTGTTGCAAGATTTCAATCAGGGGAAAAAAAGCCGTGTCTATGCCTTTGTCTCTTATCTCTCTCAAAGAGAACGTGACGCCTCTTTGTCCCTCAATGAAACCGTAAAATATGCCTCCACCTTTTTTCACTTTTTCTACTGCTTATTTCTATCTCATGCCTACTAGTCTTGTTCTTGCAGCAATTTTGTGGTCGAACTCTAGTGGTTAGAGAGGGTGTGGAGAAGAATGAGACCTAA
Protein sequence

>MS.gene024390.t1

MAANSATLCFSSFSSSSLLYHKQPLLGVGYSKRFVSFSALPYPSFSSRHSASIVENEEEQPRKLKLGNPTQVYVCNLPRSCDTQHLLHLFTPHGTVLSTEVCRNAETGESKGSAYVTMGSYNAAKNALAALDGSDVGGREMRVKFSVEMNNLETLNSPPVRRRTIYYEGPHKVYVGNLAKSATPEDVRHLFGKFGNVASVRVLQDFNQGKKSRVYAFVSYLSQRERDASLSLNETQFCGRTLVVREGVEKNET