AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93753

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93753.t1 MTR_8g107310 99.315 292 2 0 1 292 1 292 0.0 597
MS.gene93753.t1 MTR_8g031560 76.289 291 56 3 3 292 2 280 3.07e-160 447
MS.gene93753.t1 MTR_5g017130 50.000 110 52 2 185 292 68 176 1.91e-26 102
MS.gene93753.t1 MTR_4g081510 38.462 78 48 0 213 290 46 123 4.86e-11 61.2
MS.gene93753.t1 MTR_8g022050 42.857 77 44 0 212 288 148 224 6.82e-11 62.8
MS.gene93753.t1 MTR_1g080390 36.842 76 48 0 215 290 286 361 7.70e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93753.t1 AT3G11400 72.973 296 74 5 1 292 1 294 8.08e-156 436
MS.gene93753.t1 AT3G11400 66.873 323 74 5 1 292 1 321 8.46e-151 425
MS.gene93753.t1 AT5G06000 64.626 294 93 6 1 291 1 286 1.39e-132 378
MS.gene93753.t1 AT1G54080 41.111 90 50 1 206 292 140 229 6.76e-12 65.9
MS.gene93753.t1 AT3G14100 41.111 90 50 1 206 292 136 225 1.04e-11 65.5
MS.gene93753.t1 AT1G54080 40.426 94 49 2 206 292 140 233 2.12e-11 64.3

Find 107 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCAATGCTTAGGTTGTTCT+TGG 0.243434 4.2:+227751 None:intergenic
CAATAAAGGTGCTGTTCTTA+TGG 0.252283 4.2:-228226 MS.gene93753:CDS
GCGCCGTTGAGCGCCGTTCC+TGG 0.290623 4.2:-228676 MS.gene93753:CDS
GTTGCAACAGATGCTGCTCC+TGG 0.291109 4.2:-228108 MS.gene93753:CDS
ATTCGATGATGATGGTAATA+AGG 0.312148 4.2:-228756 MS.gene93753:CDS
GACAGTGGGTCAGCAGTCTT+TGG 0.317010 4.2:+228255 None:intergenic
GACTTCAAGGTGTCCATATA+AGG 0.326137 4.2:-228169 MS.gene93753:CDS
CGGAAAAGCTCAAGCAAATC+AGG 0.333826 4.2:+227943 None:intergenic
ATCAACAAACTCAATGGATA+TGG 0.334085 4.2:-227811 MS.gene93753:CDS
CCAATGCTTAGGTTGTTCTT+GGG 0.335891 4.2:+227752 None:intergenic
GTGATAGGTCCTGATGAAAA+TGG 0.346524 4.2:-228803 MS.gene93753:CDS
TCACCTGACGTGGTGGTAAC+AGG 0.357484 4.2:+228822 None:intergenic
TTATGGTATGCAGGACTTGC+GGG 0.371975 4.2:-228209 MS.gene93753:CDS
GAAGCTAAGGTGGGGAGAAT+TGG 0.373757 4.2:-228873 MS.gene93753:CDS
GGAAGACGATGGCGAAGATC+TGG 0.395348 4.2:-228849 MS.gene93753:CDS
CCACTGTCTCAATTCAATAA+AGG 0.396074 4.2:-228240 MS.gene93753:CDS
CGTCGGTGCCCGCCTCACTA+TGG 0.408913 4.2:-228624 MS.gene93753:CDS
GCTTCAGCTGCCTCAGCAAA+GGG 0.410110 4.2:-228084 MS.gene93753:CDS
ACGCGCGTTGGCAAGTTTGC+GGG 0.418864 4.2:+228709 None:intergenic
TTGCAACAGATGCTGCTCCT+GGG 0.430137 4.2:-228107 MS.gene93753:CDS
CGCCGTTCCTGGCCTAAGTT+CGG 0.430777 4.2:-228665 MS.gene93753:CDS
GGCTTCAGCTGCCTCAGCAA+AGG 0.431088 4.2:-228085 MS.gene93753:CDS
GGAGCAGCATCTGTTGCAAC+AGG 0.435145 4.2:+228111 None:intergenic
ATGCAGGACTTGCGGGAAGA+AGG 0.440149 4.2:-228202 MS.gene93753:CDS
GACGCGCGTTGGCAAGTTTG+CGG 0.443758 4.2:+228708 None:intergenic
ACTGGCATGAGTAGGGGCTT+TGG 0.446792 4.2:-227874 MS.gene93753:CDS
GCTTTGTGAACTTTGTTAGC+AGG 0.448198 4.2:-227852 MS.gene93753:CDS
TCCTCTCCGCACCAGCTCTC+AGG 0.449264 4.2:+228043 None:intergenic
GGGTGGCCCATTCAACTCTC+AGG 0.454110 4.2:+227773 None:intergenic
GCTGAGGCAGCTGAAGCCCC+AGG 0.456235 4.2:+228090 None:intergenic
GAACGATGAGAATTCTGTTC+GGG 0.459314 4.2:-227995 MS.gene93753:CDS
TTTCCTGTTACCACCACGTC+AGG 0.468496 4.2:-228825 MS.gene93753:CDS
GGCTGTGCGAGATCCTTATA+TGG 0.469555 4.2:+228156 None:intergenic
AGTCTTTGGATCCTCCTTGT+TGG 0.470984 4.2:+228269 None:intergenic
GCTGGTGCGGAGAGGACTAC+TGG 0.480564 4.2:-228036 MS.gene93753:CDS
CTGATGAAAATGGAATCAAA+AGG 0.493183 4.2:-228793 MS.gene93753:CDS
CCTTGTGTCCTCCGAGAGGT+TGG 0.493283 4.2:+227969 None:intergenic
GGCGGCAATTCAGTCTGAAC+CGG 0.495287 4.2:-228897 MS.gene93753:CDS
TGCTTAGGTTGTTCTTGGGG+TGG 0.495372 4.2:+227756 None:intergenic
TCTCATCCTGAGAGTTGAAT+GGG 0.497673 4.2:-227779 MS.gene93753:CDS
GCGGGAAGAAGGGTGATCAT+TGG 0.499819 4.2:-228191 MS.gene93753:CDS
GTGCTGTTCTTATGGTATGC+AGG 0.503047 4.2:-228218 MS.gene93753:CDS
CTGAACCGGCGAAGCTAAGG+TGG 0.503185 4.2:-228883 MS.gene93753:CDS
ATCTCATCCTGAGAGTTGAA+TGG 0.503653 4.2:-227780 MS.gene93753:CDS
TCAAAAGACTGGCATGAGTA+GGG 0.504440 4.2:-227881 MS.gene93753:CDS
TTACCACCACGTCAGGTGAT+AGG 0.508065 4.2:-228818 MS.gene93753:CDS
TCTCCCCACCTTAGCTTCGC+CGG 0.508727 4.2:+228878 None:intergenic
TGCAGGACTTGCGGGAAGAA+GGG 0.508910 4.2:-228201 MS.gene93753:CDS
GTTGCTATTGATCAAAAGAC+TGG 0.519323 4.2:-227892 MS.gene93753:CDS
CAATGCTTAGGTTGTTCTTG+GGG 0.520594 4.2:+227753 None:intergenic
AGGGTGATCATTGGACTTCA+AGG 0.520801 4.2:-228182 MS.gene93753:CDS
CTCGAACGCCCCAAACCACT+TGG 0.521196 4.2:-228581 MS.gene93753:intron
AGGGCTATCAACAAACTCAA+TGG 0.522389 4.2:-227817 MS.gene93753:CDS
GAGTACAAATTCGATGATGA+TGG 0.524262 4.2:-228764 MS.gene93753:CDS
TCCGCACCAGCTCTCAGGCT+AGG 0.528088 4.2:+228048 None:intergenic
GGTTTATCAACAAAAGTCTC+AGG 0.531499 4.2:+228132 None:intergenic
TACTGACAGCACCAAAAGGC+CGG 0.531711 4.2:+227923 None:intergenic
CTTATGGTATGCAGGACTTG+CGG 0.534636 4.2:-228210 MS.gene93753:CDS
CTTTGTGAACTTTGTTAGCA+GGG 0.538694 4.2:-227851 MS.gene93753:CDS
GGAACGATGAGAATTCTGTT+CGG 0.539699 4.2:-227996 MS.gene93753:CDS
GCGTTCGAGAAGGATCTCTT+CGG 0.539858 4.2:+228595 None:intergenic
TGCAACAGATGCTGCTCCTG+GGG 0.541734 4.2:-228106 MS.gene93753:CDS
GGCGTCTCCGAACTTAGGCC+AGG 0.544227 4.2:+228658 None:intergenic
GCTAAGGTGGGGAGAATTGG+AGG 0.546676 4.2:-228870 MS.gene93753:CDS
TGATGAAAATGGAATCAAAA+GGG 0.546960 4.2:-228792 MS.gene93753:CDS
GGCAAGTTTGCGGGTTCGAG+TGG 0.547824 4.2:+228718 None:intergenic
AGTCTGAACCGGCGAAGCTA+AGG 0.552460 4.2:-228886 MS.gene93753:CDS
GGCACCGACGTCTTCATGAA+CGG 0.554768 4.2:+228637 None:intergenic
ACCCTACTGACAGCACCAAA+AGG 0.555974 4.2:+227919 None:intergenic
AGGCCAGGAACGGCGCTCAA+CGG 0.556345 4.2:+228673 None:intergenic
GCGTTTGCTGAGACGCGCGT+TGG 0.556548 4.2:+228697 None:intergenic
CCAACCTCTCGGAGGACACA+AGG 0.558509 4.2:-227969 MS.gene93753:CDS
GGTGGAAACCATAGTGAGGC+GGG 0.568445 4.2:+228616 None:intergenic
GTGGTTTGGGGCGTTCGAGA+AGG 0.575414 4.2:+228585 None:intergenic
CTCCGAACTTAGGCCAGGAA+CGG 0.576631 4.2:+228663 None:intergenic
ATCAAAAGACTGGCATGAGT+AGG 0.579916 4.2:-227882 MS.gene93753:CDS
TCCTAGCCTGAGAGCTGGTG+CGG 0.588822 4.2:-228049 MS.gene93753:CDS
TGAACGGCGTCTCCGAACTT+AGG 0.601313 4.2:+228653 None:intergenic
CTTTATTGAATTGAGACAGT+GGG 0.603664 4.2:+228241 None:intergenic
CTGGCTCTGACATGAGGCGG+AGG 0.610847 4.2:-228017 MS.gene93753:CDS
GTACCTCCTAGCCTGAGAGC+TGG 0.611149 4.2:-228054 MS.gene93753:CDS
CAAAAGACTGGCATGAGTAG+GGG 0.615375 4.2:-227880 MS.gene93753:CDS
TGAACCGGCGAAGCTAAGGT+GGG 0.616663 4.2:-228882 MS.gene93753:CDS
GCCTGAGAGCTGGTGCGGAG+AGG 0.623849 4.2:-228044 MS.gene93753:CDS
TACATATGCACCCTTTGCTG+AGG 0.623928 4.2:+228074 None:intergenic
TAGCAGGGAAGATGCTCAGA+GGG 0.624825 4.2:-227836 MS.gene93753:CDS
TTCGAGAAGGATCTCTTCGG+TGG 0.624825 4.2:+228598 None:intergenic
CAACCTCTCGGAGGACACAA+GGG 0.627480 4.2:-227968 MS.gene93753:CDS
CGGTGGAAACCATAGTGAGG+CGG 0.629968 4.2:+228615 None:intergenic
GGACTACTGGCTCTGACATG+AGG 0.632778 4.2:-228023 MS.gene93753:CDS
TGTTCGGGTAACCAACCTCT+CGG 0.633411 4.2:-227980 MS.gene93753:CDS
AATCTAATCAGACATGGCGG+CGG 0.647531 4.2:-228915 None:intergenic
GCACCAGCTCTCAGGCTAGG+AGG 0.652161 4.2:+228051 None:intergenic
GAACCGGCGAAGCTAAGGTG+GGG 0.653069 4.2:-228881 MS.gene93753:CDS
CCTTTATTGAATTGAGACAG+TGG 0.657041 4.2:+228240 None:intergenic
CTACTGGCTCTGACATGAGG+CGG 0.663022 4.2:-228020 MS.gene93753:CDS
GCTCCCTTGTGTCCTCCGAG+AGG 0.679337 4.2:+227965 None:intergenic
GGACCTATCACCTGACGTGG+TGG 0.679711 4.2:+228815 None:intergenic
TTATCAACAAAAGTCTCAGG+CGG 0.681859 4.2:+228135 None:intergenic
TTAGCAGGGAAGATGCTCAG+AGG 0.686366 4.2:-227837 MS.gene93753:CDS
ATCAACAACAGGTTCCAACA+AGG 0.686562 4.2:-228283 MS.gene93753:intron
GGAGAATTGGAGGAAGACGA+TGG 0.696314 4.2:-228860 MS.gene93753:CDS
TCAGGACCTATCACCTGACG+TGG 0.706707 4.2:+228812 None:intergenic
AACAACAGGTTCCAACAAGG+AGG 0.706807 4.2:-228280 MS.gene93753:intron
CTTCGGTGGAAACCATAGTG+AGG 0.709928 4.2:+228612 None:intergenic
TCGGGTAACCAACCTCTCGG+AGG 0.730211 4.2:-227977 MS.gene93753:CDS
GACGCCGTTCATGAAGACGT+CGG 0.745318 4.2:-228641 MS.gene93753:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACTTTAGATGATGAATTACT+GGG - chr4.2:228248-228267 MS.gene93753:CDS 25.0%
! TACTTTAGATGATGAATTAC+TGG - chr4.2:228247-228266 MS.gene93753:CDS 25.0%
! TGATGAAAATGGAATCAAAA+GGG - chr4.2:227869-227888 MS.gene93753:CDS 25.0%
!! GTAATTCATCATCTAAAGTA+AGG + chr4.2:228247-228266 None:intergenic 25.0%
ATCAACAAACTCAATGGATA+TGG - chr4.2:228850-228869 MS.gene93753:CDS 30.0%
CTGATGAAAATGGAATCAAA+AGG - chr4.2:227868-227887 MS.gene93753:CDS 30.0%
CTTTATTGAATTGAGACAGT+GGG + chr4.2:228423-228442 None:intergenic 30.0%
! TTCATCATCTAAAGTAAGGA+AGG + chr4.2:228243-228262 None:intergenic 30.0%
!! ATTCGATGATGATGGTAATA+AGG - chr4.2:227905-227924 MS.gene93753:CDS 30.0%
!! CTTTTGATTCCATTTTCATC+AGG + chr4.2:227870-227889 None:intergenic 30.0%
!! TAAAGAGAAAAAGTACCAAG+TGG + chr4.2:228098-228117 None:intergenic 30.0%
ATGCTACAATAGACTAGACT+AGG + chr4.2:228329-228348 None:intergenic 35.0%
CACAATCACACAAGAATGTT+CGG - chr4.2:228198-228217 MS.gene93753:CDS 35.0%
CCACTGTCTCAATTCAATAA+AGG - chr4.2:228421-228440 MS.gene93753:intron 35.0%
CCTTTATTGAATTGAGACAG+TGG + chr4.2:228424-228443 None:intergenic 35.0%
CTTTGTGAACTTTGTTAGCA+GGG - chr4.2:228810-228829 MS.gene93753:CDS 35.0%
GAGTACAAATTCGATGATGA+TGG - chr4.2:227897-227916 MS.gene93753:CDS 35.0%
GGTTTATCAACAAAAGTCTC+AGG + chr4.2:228532-228551 None:intergenic 35.0%
TCATGATCTGTATCAACAAC+AGG - chr4.2:228367-228386 MS.gene93753:intron 35.0%
TTATCAACAAAAGTCTCAGG+CGG + chr4.2:228529-228548 None:intergenic 35.0%
! GTTGCTATTGATCAAAAGAC+TGG - chr4.2:228769-228788 MS.gene93753:CDS 35.0%
!! AAAAAGTACCAAGTGGTTTG+GGG + chr4.2:228091-228110 None:intergenic 35.0%
!! AGAAAAAGTACCAAGTGGTT+TGG + chr4.2:228093-228112 None:intergenic 35.0%
!! CAATAAAGGTGCTGTTCTTA+TGG - chr4.2:228435-228454 MS.gene93753:intron 35.0%
!! GAAAAAGTACCAAGTGGTTT+GGG + chr4.2:228092-228111 None:intergenic 35.0%
AGGGCTATCAACAAACTCAA+TGG - chr4.2:228844-228863 MS.gene93753:CDS 40.0%
ATCAAAAGACTGGCATGAGT+AGG - chr4.2:228779-228798 MS.gene93753:CDS 40.0%
ATCAACAACAGGTTCCAACA+AGG - chr4.2:228378-228397 MS.gene93753:intron 40.0%
ATCTCATCCTGAGAGTTGAA+TGG - chr4.2:228881-228900 MS.gene93753:CDS 40.0%
GAACGATGAGAATTCTGTTC+GGG - chr4.2:228666-228685 MS.gene93753:CDS 40.0%
GCTTTGTGAACTTTGTTAGC+AGG - chr4.2:228809-228828 MS.gene93753:CDS 40.0%
GGAACGATGAGAATTCTGTT+CGG - chr4.2:228665-228684 MS.gene93753:CDS 40.0%
GTGATAGGTCCTGATGAAAA+TGG - chr4.2:227858-227877 MS.gene93753:CDS 40.0%
TCAAAAGACTGGCATGAGTA+GGG - chr4.2:228780-228799 MS.gene93753:CDS 40.0%
TCTCATCCTGAGAGTTGAAT+GGG - chr4.2:228882-228901 MS.gene93753:CDS 40.0%
! GACTTCAAGGTGTCCATATA+AGG - chr4.2:228492-228511 MS.gene93753:intron 40.0%
!!! CTGATTTGCTTGAGCTTTTC+CGG - chr4.2:228719-228738 MS.gene93753:CDS 40.0%
AACAACAGGTTCCAACAAGG+AGG - chr4.2:228381-228400 MS.gene93753:intron 45.0%
ACTAGTTATCTAGCGACCAG+GGG - chr4.2:228170-228189 MS.gene93753:CDS 45.0%
AGGGTGATCATTGGACTTCA+AGG - chr4.2:228479-228498 MS.gene93753:intron 45.0%
AGTCTTTGGATCCTCCTTGT+TGG + chr4.2:228395-228414 None:intergenic 45.0%
CAAAAGACTGGCATGAGTAG+GGG - chr4.2:228781-228800 MS.gene93753:CDS 45.0%
CGGAAAAGCTCAAGCAAATC+AGG + chr4.2:228721-228740 None:intergenic 45.0%
CTTATGGTATGCAGGACTTG+CGG - chr4.2:228451-228470 MS.gene93753:intron 45.0%
GTGTGATTGTGTAAATCCCC+TGG + chr4.2:228189-228208 None:intergenic 45.0%
TACATATGCACCCTTTGCTG+AGG + chr4.2:228590-228609 None:intergenic 45.0%
TTATGGTATGCAGGACTTGC+GGG - chr4.2:228452-228471 MS.gene93753:intron 45.0%
! AGACTAGTTATCTAGCGACC+AGG - chr4.2:228168-228187 MS.gene93753:CDS 45.0%
! GACTAGTTATCTAGCGACCA+GGG - chr4.2:228169-228188 MS.gene93753:CDS 45.0%
! GTGCTGTTCTTATGGTATGC+AGG - chr4.2:228443-228462 MS.gene93753:intron 45.0%
ACCCTACTGACAGCACCAAA+AGG + chr4.2:228745-228764 None:intergenic 50.0%
CTTCGGTGGAAACCATAGTG+AGG + chr4.2:228052-228071 None:intergenic 50.0%
GAAGCTAAGGTGGGGAGAAT+TGG - chr4.2:227788-227807 MS.gene93753:CDS 50.0%
GCGTTCGAGAAGGATCTCTT+CGG + chr4.2:228069-228088 None:intergenic 50.0%
GGAGAATTGGAGGAAGACGA+TGG - chr4.2:227801-227820 MS.gene93753:CDS 50.0%
GGCTGTGCGAGATCCTTATA+TGG + chr4.2:228508-228527 None:intergenic 50.0%
TACTGACAGCACCAAAAGGC+CGG + chr4.2:228741-228760 None:intergenic 50.0%
TAGCAGGGAAGATGCTCAGA+GGG - chr4.2:228825-228844 MS.gene93753:CDS 50.0%
TGTTCGGGTAACCAACCTCT+CGG - chr4.2:228681-228700 MS.gene93753:CDS 50.0%
TTACCACCACGTCAGGTGAT+AGG - chr4.2:227843-227862 MS.gene93753:CDS 50.0%
TTAGCAGGGAAGATGCTCAG+AGG - chr4.2:228824-228843 MS.gene93753:CDS 50.0%
TTCGAGAAGGATCTCTTCGG+TGG + chr4.2:228066-228085 None:intergenic 50.0%
TTGCAACAGATGCTGCTCCT+GGG - chr4.2:228554-228573 MS.gene93753:intron 50.0%
TTTCCTGTTACCACCACGTC+AGG - chr4.2:227836-227855 MS.gene93753:CDS 50.0%
! CTTGAGCTTTTCCGGCCTTT+TGG - chr4.2:228727-228746 MS.gene93753:CDS 50.0%
!!! GCCTTTTGGTGCTGTCAGTA+GGG - chr4.2:228741-228760 MS.gene93753:CDS 50.0%
ACTGGCATGAGTAGGGGCTT+TGG - chr4.2:228787-228806 MS.gene93753:CDS 55.0%
AGTCTGAACCGGCGAAGCTA+AGG - chr4.2:227775-227794 MS.gene93753:CDS 55.0%
ATGCAGGACTTGCGGGAAGA+AGG - chr4.2:228459-228478 MS.gene93753:intron 55.0%
CAACCTCTCGGAGGACACAA+GGG - chr4.2:228693-228712 MS.gene93753:CDS 55.0%
CGGTGGAAACCATAGTGAGG+CGG + chr4.2:228049-228068 None:intergenic 55.0%
CTCCGAACTTAGGCCAGGAA+CGG + chr4.2:228001-228020 None:intergenic 55.0%
GACAGTGGGTCAGCAGTCTT+TGG + chr4.2:228409-228428 None:intergenic 55.0%
GACGCCGTTCATGAAGACGT+CGG - chr4.2:228020-228039 MS.gene93753:CDS 55.0%
GCGGGAAGAAGGGTGATCAT+TGG - chr4.2:228470-228489 MS.gene93753:intron 55.0%
GCTAAGGTGGGGAGAATTGG+AGG - chr4.2:227791-227810 MS.gene93753:CDS 55.0%
GCTTCAGCTGCCTCAGCAAA+GGG - chr4.2:228577-228596 MS.gene93753:intron 55.0%
GGAAGACGATGGCGAAGATC+TGG - chr4.2:227812-227831 MS.gene93753:CDS 55.0%
GGAGCAGCATCTGTTGCAAC+AGG + chr4.2:228553-228572 None:intergenic 55.0%
GGCGGCAATTCAGTCTGAAC+CGG - chr4.2:227764-227783 MS.gene93753:CDS 55.0%
GGTGGAAACCATAGTGAGGC+GGG + chr4.2:228048-228067 None:intergenic 55.0%
GTTGCAACAGATGCTGCTCC+TGG - chr4.2:228553-228572 MS.gene93753:intron 55.0%
TCACCTGACGTGGTGGTAAC+AGG + chr4.2:227842-227861 None:intergenic 55.0%
TCAGGACCTATCACCTGACG+TGG + chr4.2:227852-227871 None:intergenic 55.0%
TGAACCGGCGAAGCTAAGGT+GGG - chr4.2:227779-227798 MS.gene93753:CDS 55.0%
TGAACGGCGTCTCCGAACTT+AGG + chr4.2:228011-228030 None:intergenic 55.0%
TGCAACAGATGCTGCTCCTG+GGG - chr4.2:228555-228574 MS.gene93753:intron 55.0%
TGCAGGACTTGCGGGAAGAA+GGG - chr4.2:228460-228479 MS.gene93753:intron 55.0%
!! CTACTGGCTCTGACATGAGG+CGG - chr4.2:228641-228660 MS.gene93753:CDS 55.0%
!! GGACTACTGGCTCTGACATG+AGG - chr4.2:228638-228657 MS.gene93753:CDS 55.0%
!! GGCACCGACGTCTTCATGAA+CGG + chr4.2:228027-228046 None:intergenic 55.0%
!!! GGCCTTTTGGTGCTGTCAGT+AGG - chr4.2:228740-228759 MS.gene93753:CDS 55.0%
CCAACCTCTCGGAGGACACA+AGG - chr4.2:228692-228711 MS.gene93753:CDS 60.0%
CCTTGTGTCCTCCGAGAGGT+TGG + chr4.2:228695-228714 None:intergenic 60.0%
CGCCGTTCCTGGCCTAAGTT+CGG - chr4.2:227996-228015 MS.gene93753:CDS 60.0%
CTCGAACGCCCCAAACCACT+TGG - chr4.2:228080-228099 MS.gene93753:CDS 60.0%
CTGAACCGGCGAAGCTAAGG+TGG - chr4.2:227778-227797 MS.gene93753:CDS 60.0%
GAACCGGCGAAGCTAAGGTG+GGG - chr4.2:227780-227799 MS.gene93753:CDS 60.0%
GGACCTATCACCTGACGTGG+TGG + chr4.2:227849-227868 None:intergenic 60.0%
GGCAAGTTTGCGGGTTCGAG+TGG + chr4.2:227946-227965 None:intergenic 60.0%
GGCTTCAGCTGCCTCAGCAA+AGG - chr4.2:228576-228595 MS.gene93753:intron 60.0%
GGGTGGCCCATTCAACTCTC+AGG + chr4.2:228891-228910 None:intergenic 60.0%
GTACCTCCTAGCCTGAGAGC+TGG - chr4.2:228607-228626 MS.gene93753:CDS 60.0%
TCCTAGCCTGAGAGCTGGTG+CGG - chr4.2:228612-228631 MS.gene93753:CDS 60.0%
TCGGGTAACCAACCTCTCGG+AGG - chr4.2:228684-228703 MS.gene93753:CDS 60.0%
TCTCCCCACCTTAGCTTCGC+CGG + chr4.2:227786-227805 None:intergenic 60.0%
! ACGCGCGTTGGCAAGTTTGC+GGG + chr4.2:227955-227974 None:intergenic 60.0%
! GACGCGCGTTGGCAAGTTTG+CGG + chr4.2:227956-227975 None:intergenic 60.0%
! GTGGTTTGGGGCGTTCGAGA+AGG + chr4.2:228079-228098 None:intergenic 60.0%
AGGCCAGGAACGGCGCTCAA+CGG + chr4.2:227991-228010 None:intergenic 65.0%
GCACCAGCTCTCAGGCTAGG+AGG + chr4.2:228613-228632 None:intergenic 65.0%
GCTCCCTTGTGTCCTCCGAG+AGG + chr4.2:228699-228718 None:intergenic 65.0%
GCTGGTGCGGAGAGGACTAC+TGG - chr4.2:228625-228644 MS.gene93753:CDS 65.0%
GGCGTCTCCGAACTTAGGCC+AGG + chr4.2:228006-228025 None:intergenic 65.0%
TCCGCACCAGCTCTCAGGCT+AGG + chr4.2:228616-228635 None:intergenic 65.0%
TCCTCTCCGCACCAGCTCTC+AGG + chr4.2:228621-228640 None:intergenic 65.0%
! GCGTTTGCTGAGACGCGCGT+TGG + chr4.2:227967-227986 None:intergenic 65.0%
!! CTGGCTCTGACATGAGGCGG+AGG - chr4.2:228644-228663 MS.gene93753:CDS 65.0%
GCCTGAGAGCTGGTGCGGAG+AGG - chr4.2:228617-228636 MS.gene93753:CDS 70.0%
GCTGAGGCAGCTGAAGCCCC+AGG + chr4.2:228574-228593 None:intergenic 70.0%
! CGTCGGTGCCCGCCTCACTA+TGG - chr4.2:228037-228056 MS.gene93753:CDS 70.0%
! GCGCCGTTGAGCGCCGTTCC+TGG - chr4.2:227985-228004 MS.gene93753:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 227759 228924 227759 ID=MS.gene93753
chr4.2 mRNA 227759 228924 227759 ID=MS.gene93753.t1;Parent=MS.gene93753
chr4.2 exon 228582 228924 228582 ID=MS.gene93753.t1.exon1;Parent=MS.gene93753.t1
chr4.2 CDS 228582 228924 228582 ID=cds.MS.gene93753.t1;Parent=MS.gene93753.t1
chr4.2 exon 227759 228294 227759 ID=MS.gene93753.t1.exon2;Parent=MS.gene93753.t1
chr4.2 CDS 227759 228294 227759 ID=cds.MS.gene93753.t1;Parent=MS.gene93753.t1
Gene Sequence

>MS.gene93753

ATGGCGGCGGCAATTCAGTCTGAACCGGCGAAGCTAAGGTGGGGAGAATTGGAGGAAGACGATGGCGAAGATCTGGATTTCCTGTTACCACCACGTCAGGTGATAGGTCCTGATGAAAATGGAATCAAAAGGGTTATTGAGTACAAATTCGATGATGATGGTAATAAGGTTAAGATCACTACAACCACTCGAACCCGCAAACTTGCCAACGCGCGTCTCAGCAAACGCGCCGTTGAGCGCCGTTCCTGGCCTAAGTTCGGAGACGCCGTTCATGAAGACGTCGGTGCCCGCCTCACTATGGTTTCCACCGAAGAGATCCTTCTCGAACGCCCCAAACCACTTGGTACTTTTTCTCTTTATCTTTCTCTTTCTAGAGATGCTACATTTCAATTTCAATTCAATTGAATCTAGACTAGTTATCTAGCGACCAGGGGATTTACACAATCACACAAGAATGTTCGGATTGAATCTATACAAAATTCCTTCCTTACTTTAGATGATGAATTACTGGGATTTCTTGCATACACATGAATTTAGCTTAGTTGTCTTAATTAATTTCACTTTCAACCTAGTCTAGTCTATTGTAGCATTTGTCTATCAATCTCATCTCATGATCTGTATCAACAACAGGTTCCAACAAGGAGGATCCAAAGACTGCTGACCCACTGTCTCAATTCAATAAAGGTGCTGTTCTTATGGTATGCAGGACTTGCGGGAAGAAGGGTGATCATTGGACTTCAAGGTGTCCATATAAGGATCTCGCACAGCCGCCTGAGACTTTTGTTGATAAACCTGTTGCAACAGATGCTGCTCCTGGGGCTTCAGCTGCCTCAGCAAAGGGTGCATATGTACCTCCTAGCCTGAGAGCTGGTGCGGAGAGGACTACTGGCTCTGACATGAGGCGGAGGAACGATGAGAATTCTGTTCGGGTAACCAACCTCTCGGAGGACACAAGGGAGCCTGATTTGCTTGAGCTTTTCCGGCCTTTTGGTGCTGTCAGTAGGGTCTATGTTGCTATTGATCAAAAGACTGGCATGAGTAGGGGCTTTGGCTTTGTGAACTTTGTTAGCAGGGAAGATGCTCAGAGGGCTATCAACAAACTCAATGGATATGGCTACGACAATCTCATCCTGAGAGTTGAATGGGCCACCCCAAGAACAACCTAA
Protein sequence

>MS.gene93753.t1

MAAAIQSEPAKLRWGELEEDDGEDLDFLLPPRQVIGPDENGIKRVIEYKFDDDGNKVKITTTTRTRKLANARLSKRAVERRSWPKFGDAVHEDVGARLTMVSTEEILLERPKPLGSNKEDPKTADPLSQFNKGAVLMVCRTCGKKGDHWTSRCPYKDLAQPPETFVDKPVATDAAPGASAASAKGAYVPPSLRAGAERTTGSDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAIDQKTGMSRGFGFVNFVSREDAQRAINKLNGYGYDNLILRVEWATPRTT