AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene040115

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040115.t1 MTR_8g107310 99.315 292 2 0 1 292 1 292 0.0 597
MS.gene040115.t1 MTR_8g031560 76.289 291 56 3 3 292 2 280 3.07e-160 447
MS.gene040115.t1 MTR_5g017130 50.000 110 52 2 185 292 68 176 1.91e-26 102
MS.gene040115.t1 MTR_4g081510 38.462 78 48 0 213 290 46 123 4.86e-11 61.2
MS.gene040115.t1 MTR_8g022050 42.857 77 44 0 212 288 148 224 6.82e-11 62.8
MS.gene040115.t1 MTR_1g080390 36.842 76 48 0 215 290 286 361 7.70e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040115.t1 AT3G11400 72.973 296 74 5 1 292 1 294 8.08e-156 436
MS.gene040115.t1 AT3G11400 66.873 323 74 5 1 292 1 321 8.46e-151 425
MS.gene040115.t1 AT5G06000 64.626 294 93 6 1 291 1 286 1.39e-132 378
MS.gene040115.t1 AT1G54080 41.111 90 50 1 206 292 140 229 6.76e-12 65.9
MS.gene040115.t1 AT3G14100 41.111 90 50 1 206 292 136 225 1.04e-11 65.5
MS.gene040115.t1 AT1G54080 40.426 94 49 2 206 292 140 233 2.12e-11 64.3

Find 107 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCAATGCTTAGGTTGTTCT+TGG 0.243434 4.4:+288298 None:intergenic
CAATAAAGGTGCTGTTCTTA+TGG 0.252283 4.4:-288773 MS.gene040115:CDS
GCGCCGTTGAGCGCCGTTCC+TGG 0.290623 4.4:-289206 MS.gene040115:CDS
GTTGCAACAGATGCTGCTCC+TGG 0.291109 4.4:-288655 MS.gene040115:CDS
ATTCGATGATGATGGTAATA+AGG 0.312148 4.4:-289286 MS.gene040115:CDS
GACTTCAAGGTGTCCATATA+AGG 0.326137 4.4:-288716 MS.gene040115:CDS
GAGAGTGGGTCAGCAGTCTT+TGG 0.333733 4.4:+288802 None:intergenic
CGGAAAAGCTCAAGCAAATC+AGG 0.333826 4.4:+288490 None:intergenic
ATCAACAAACTCAATGGATA+TGG 0.334085 4.4:-288358 MS.gene040115:CDS
CCAATGCTTAGGTTGTTCTT+GGG 0.335891 4.4:+288299 None:intergenic
GTGATAGGTCCTGATGAAAA+TGG 0.346524 4.4:-289333 MS.gene040115:CDS
TCACCTGACGTGGTGGTAAC+AGG 0.357484 4.4:+289352 None:intergenic
CCACTCTCTCAATTCAATAA+AGG 0.371242 4.4:-288787 MS.gene040115:CDS
TTATGGTATGCAGGACTTGC+GGG 0.371975 4.4:-288756 MS.gene040115:CDS
GAAGCTAAGGTGGGGAGAAT+TGG 0.373757 4.4:-289403 MS.gene040115:CDS
GGAAGACGATGGCGAAGATC+TGG 0.395348 4.4:-289379 MS.gene040115:CDS
CGTCGGTGCCCGCCTCACTA+TGG 0.408913 4.4:-289154 MS.gene040115:CDS
GCTTCAGCTGCCTCAGCAAA+GGG 0.410110 4.4:-288631 MS.gene040115:CDS
ACGCGCGTTGGCAAGTTTGC+GGG 0.418864 4.4:+289239 None:intergenic
TTGCAACAGATGCTGCTCCT+GGG 0.430137 4.4:-288654 MS.gene040115:CDS
CGCCGTTCCTGGCCTAAGTT+CGG 0.430777 4.4:-289195 MS.gene040115:CDS
GGCTTCAGCTGCCTCAGCAA+AGG 0.431088 4.4:-288632 MS.gene040115:CDS
GGAGCAGCATCTGTTGCAAC+AGG 0.435145 4.4:+288658 None:intergenic
ATGCAGGACTTGCGGGAAGA+AGG 0.440149 4.4:-288749 MS.gene040115:CDS
GCTTTGTCAACTTTGTTAGC+AGG 0.441627 4.4:-288399 MS.gene040115:CDS
GACGCGCGTTGGCAAGTTTG+CGG 0.443758 4.4:+289238 None:intergenic
ACTGGCATGAGTAGGGGCTT+TGG 0.446792 4.4:-288421 MS.gene040115:CDS
TCCTCTCCGCACCAGCTCTC+AGG 0.449264 4.4:+288590 None:intergenic
GGGTGGCCCATTCAACTCTC+AGG 0.454110 4.4:+288320 None:intergenic
GCTGAGGCAGCTGAAGCCCC+AGG 0.456235 4.4:+288637 None:intergenic
GAACGATGAGAATTCTGTTC+GGG 0.459314 4.4:-288542 MS.gene040115:CDS
TTTCCTGTTACCACCACGTC+AGG 0.468496 4.4:-289355 MS.gene040115:CDS
GGCTGTGCGAGATCCTTATA+TGG 0.469555 4.4:+288703 None:intergenic
AGTCTTTGGATCCTCCTTGT+TGG 0.470984 4.4:+288816 None:intergenic
CTGATGAAAATGGAATCAAA+AGG 0.493183 4.4:-289323 MS.gene040115:CDS
CCTTGTGTCCTCCGAGAGGT+TGG 0.493283 4.4:+288516 None:intergenic
GGCGGCAATTCAGTCTGAAC+CGG 0.495287 4.4:-289427 MS.gene040115:CDS
TGCTTAGGTTGTTCTTGGGG+TGG 0.495372 4.4:+288303 None:intergenic
TCTCATCCTGAGAGTTGAAT+GGG 0.497673 4.4:-288326 MS.gene040115:CDS
GCGGGAAGAAGGGTGATCAT+TGG 0.499819 4.4:-288738 MS.gene040115:CDS
GTGCTGTTCTTATGGTATGC+AGG 0.503047 4.4:-288765 MS.gene040115:CDS
CTGAACCGGCGAAGCTAAGG+TGG 0.503185 4.4:-289413 MS.gene040115:CDS
ATCTCATCCTGAGAGTTGAA+TGG 0.503653 4.4:-288327 MS.gene040115:CDS
TCAAAAGACTGGCATGAGTA+GGG 0.504440 4.4:-288428 MS.gene040115:CDS
TTACCACCACGTCAGGTGAT+AGG 0.508065 4.4:-289348 MS.gene040115:CDS
TCTCCCCACCTTAGCTTCGC+CGG 0.508727 4.4:+289408 None:intergenic
TGCAGGACTTGCGGGAAGAA+GGG 0.508910 4.4:-288748 MS.gene040115:CDS
GTTGCTATTGATCAAAAGAC+TGG 0.519323 4.4:-288439 MS.gene040115:CDS
CAATGCTTAGGTTGTTCTTG+GGG 0.520594 4.4:+288300 None:intergenic
AGGGTGATCATTGGACTTCA+AGG 0.520801 4.4:-288729 MS.gene040115:CDS
CTCGAACGCCCCAAACCACT+TGG 0.521196 4.4:-289111 MS.gene040115:intron
AGGGCTATCAACAAACTCAA+TGG 0.522389 4.4:-288364 MS.gene040115:CDS
GAGTACAAATTCGATGATGA+TGG 0.524262 4.4:-289294 MS.gene040115:CDS
TCCGCACCAGCTCTCAGGCT+AGG 0.528088 4.4:+288595 None:intergenic
GGTTTATCAACAAAAGTCTC+AGG 0.531499 4.4:+288679 None:intergenic
TACTGACAGCACCAAAAGGC+CGG 0.531711 4.4:+288470 None:intergenic
CTTATGGTATGCAGGACTTG+CGG 0.534636 4.4:-288757 MS.gene040115:CDS
CTTTGTCAACTTTGTTAGCA+GGG 0.538694 4.4:-288398 MS.gene040115:CDS
GGAACGATGAGAATTCTGTT+CGG 0.539699 4.4:-288543 MS.gene040115:CDS
GCGTTCGAGAAGGATCTCTT+CGG 0.539858 4.4:+289125 None:intergenic
TGCAACAGATGCTGCTCCTG+GGG 0.541734 4.4:-288653 MS.gene040115:CDS
GGCGTCTCCGAACTTAGGCC+AGG 0.544227 4.4:+289188 None:intergenic
GCTAAGGTGGGGAGAATTGG+AGG 0.546676 4.4:-289400 MS.gene040115:CDS
TGATGAAAATGGAATCAAAA+GGG 0.546960 4.4:-289322 MS.gene040115:CDS
GGCAAGTTTGCGGGTTCGAG+TGG 0.547824 4.4:+289248 None:intergenic
GCTGGTGCGGAGAGGACTAC+CGG 0.551357 4.4:-288583 MS.gene040115:CDS
AGTCTGAACCGGCGAAGCTA+AGG 0.552460 4.4:-289416 MS.gene040115:CDS
GGCACCGACGTCTTCATGAA+CGG 0.554768 4.4:+289167 None:intergenic
ACCCTACTGACAGCACCAAA+AGG 0.555974 4.4:+288466 None:intergenic
AGGCCAGGAACGGCGCTCAA+CGG 0.556345 4.4:+289203 None:intergenic
GCGTTTGCTGAGACGCGCGT+TGG 0.556548 4.4:+289227 None:intergenic
CCAACCTCTCGGAGGACACA+AGG 0.558509 4.4:-288516 MS.gene040115:CDS
GGTGGAAACCATAGTGAGGC+GGG 0.568445 4.4:+289146 None:intergenic
GTGGTTTGGGGCGTTCGAGA+AGG 0.575414 4.4:+289115 None:intergenic
CTCCGAACTTAGGCCAGGAA+CGG 0.576631 4.4:+289193 None:intergenic
ATCAAAAGACTGGCATGAGT+AGG 0.579916 4.4:-288429 MS.gene040115:CDS
CCTTTATTGAATTGAGAGAG+TGG 0.580795 4.4:+288787 None:intergenic
TCCTAGCCTGAGAGCTGGTG+CGG 0.588822 4.4:-288596 MS.gene040115:CDS
CCTCCGTCTCATGTCAGAGC+CGG 0.590579 4.4:+288564 None:intergenic
CTTTATTGAATTGAGAGAGT+GGG 0.594853 4.4:+288788 None:intergenic
TGAACGGCGTCTCCGAACTT+AGG 0.601313 4.4:+289183 None:intergenic
GTACCTCCTAGCCTGAGAGC+TGG 0.611149 4.4:-288601 MS.gene040115:CDS
CAAAAGACTGGCATGAGTAG+GGG 0.615375 4.4:-288427 MS.gene040115:CDS
TGAACCGGCGAAGCTAAGGT+GGG 0.616663 4.4:-289412 MS.gene040115:CDS
CTACCGGCTCTGACATGAGA+CGG 0.623482 4.4:-288567 MS.gene040115:CDS
GCCTGAGAGCTGGTGCGGAG+AGG 0.623849 4.4:-288591 MS.gene040115:CDS
TACATATGCACCCTTTGCTG+AGG 0.623928 4.4:+288621 None:intergenic
TAGCAGGGAAGATGCTCAGA+GGG 0.624825 4.4:-288383 MS.gene040115:CDS
TTCGAGAAGGATCTCTTCGG+TGG 0.624825 4.4:+289128 None:intergenic
CAACCTCTCGGAGGACACAA+GGG 0.627480 4.4:-288515 MS.gene040115:CDS
CGGTGGAAACCATAGTGAGG+CGG 0.629968 4.4:+289145 None:intergenic
TGTTCGGGTAACCAACCTCT+CGG 0.633411 4.4:-288527 MS.gene040115:CDS
AATCTAATCAGACATGGCGG+CGG 0.647531 4.4:-289445 None:intergenic
GCACCAGCTCTCAGGCTAGG+AGG 0.652161 4.4:+288598 None:intergenic
GAACCGGCGAAGCTAAGGTG+GGG 0.653069 4.4:-289411 MS.gene040115:CDS
GCTCCCTTGTGTCCTCCGAG+AGG 0.679337 4.4:+288512 None:intergenic
GGACCTATCACCTGACGTGG+TGG 0.679711 4.4:+289345 None:intergenic
TTATCAACAAAAGTCTCAGG+CGG 0.681859 4.4:+288682 None:intergenic
TTAGCAGGGAAGATGCTCAG+AGG 0.686366 4.4:-288384 MS.gene040115:CDS
ATCAACAACAGGTTCCAACA+AGG 0.686562 4.4:-288830 MS.gene040115:intron
GGAGAATTGGAGGAAGACGA+TGG 0.696314 4.4:-289390 MS.gene040115:CDS
TCAGGACCTATCACCTGACG+TGG 0.706707 4.4:+289342 None:intergenic
AACAACAGGTTCCAACAAGG+AGG 0.706807 4.4:-288827 MS.gene040115:intron
CTTCGGTGGAAACCATAGTG+AGG 0.709928 4.4:+289142 None:intergenic
CCGGCTCTGACATGAGACGG+AGG 0.712614 4.4:-288564 MS.gene040115:CDS
TCGGGTAACCAACCTCTCGG+AGG 0.730211 4.4:-288524 MS.gene040115:CDS
GACGCCGTTCATGAAGACGT+CGG 0.745318 4.4:-289171 MS.gene040115:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGATGAAAATGGAATCAAAA+GGG - chr4.4:288416-288435 MS.gene040115:CDS 25.0%
ATCAACAAACTCAATGGATA+TGG - chr4.4:289380-289399 MS.gene040115:CDS 30.0%
ATCTATACAAAATTCCTTCC+TGG - chr4.4:288777-288796 MS.gene040115:CDS 30.0%
CTGATGAAAATGGAATCAAA+AGG - chr4.4:288415-288434 MS.gene040115:CDS 30.0%
CTTTATTGAATTGAGAGAGT+GGG + chr4.4:288953-288972 None:intergenic 30.0%
TCTATACAAAATTCCTTCCT+GGG - chr4.4:288778-288797 MS.gene040115:CDS 30.0%
!! ATTCGATGATGATGGTAATA+AGG - chr4.4:288452-288471 MS.gene040115:CDS 30.0%
!! CTTTTGATTCCATTTTCATC+AGG + chr4.4:288417-288436 None:intergenic 30.0%
AACCTAGTCTAGTCTATTGT+AGG - chr4.4:288854-288873 MS.gene040115:intron 35.0%
ATCCTACAATAGACTAGACT+AGG + chr4.4:288859-288878 None:intergenic 35.0%
CACAATCACACAAGAATGTT+CGG - chr4.4:288749-288768 MS.gene040115:CDS 35.0%
CCACTCTCTCAATTCAATAA+AGG - chr4.4:288951-288970 MS.gene040115:intron 35.0%
CCTTTATTGAATTGAGAGAG+TGG + chr4.4:288954-288973 None:intergenic 35.0%
CTTTGTCAACTTTGTTAGCA+GGG - chr4.4:289340-289359 MS.gene040115:CDS 35.0%
GAGTACAAATTCGATGATGA+TGG - chr4.4:288444-288463 MS.gene040115:CDS 35.0%
GGTTTATCAACAAAAGTCTC+AGG + chr4.4:289062-289081 None:intergenic 35.0%
TCATGATCTGTATCAACAAC+AGG - chr4.4:288897-288916 MS.gene040115:intron 35.0%
TTATCAACAAAAGTCTCAGG+CGG + chr4.4:289059-289078 None:intergenic 35.0%
! GTTGCTATTGATCAAAAGAC+TGG - chr4.4:289299-289318 MS.gene040115:CDS 35.0%
!! AAAAAGTACCAAGTGGTTTG+GGG + chr4.4:288638-288657 None:intergenic 35.0%
!! AGAAAAAGTACCAAGTGGTT+TGG + chr4.4:288640-288659 None:intergenic 35.0%
!! CAATAAAGGTGCTGTTCTTA+TGG - chr4.4:288965-288984 MS.gene040115:intron 35.0%
!! GAAAAAGTACCAAGTGGTTT+GGG + chr4.4:288639-288658 None:intergenic 35.0%
!! GAAAGAGAAAAAGTACCAAG+TGG + chr4.4:288645-288664 None:intergenic 35.0%
AGGGCTATCAACAAACTCAA+TGG - chr4.4:289374-289393 MS.gene040115:CDS 40.0%
ATCAAAAGACTGGCATGAGT+AGG - chr4.4:289309-289328 MS.gene040115:CDS 40.0%
ATCAACAACAGGTTCCAACA+AGG - chr4.4:288908-288927 MS.gene040115:intron 40.0%
ATCTCATCCTGAGAGTTGAA+TGG - chr4.4:289411-289430 MS.gene040115:CDS 40.0%
ATGTGTATGCAAGAAATCCC+AGG + chr4.4:288798-288817 None:intergenic 40.0%
GAACGATGAGAATTCTGTTC+GGG - chr4.4:289196-289215 MS.gene040115:CDS 40.0%
GCTTTGTCAACTTTGTTAGC+AGG - chr4.4:289339-289358 MS.gene040115:CDS 40.0%
GGAACGATGAGAATTCTGTT+CGG - chr4.4:289195-289214 MS.gene040115:CDS 40.0%
GTGATAGGTCCTGATGAAAA+TGG - chr4.4:288405-288424 MS.gene040115:CDS 40.0%
TCAAAAGACTGGCATGAGTA+GGG - chr4.4:289310-289329 MS.gene040115:CDS 40.0%
TCTCATCCTGAGAGTTGAAT+GGG - chr4.4:289412-289431 MS.gene040115:CDS 40.0%
! GACTTCAAGGTGTCCATATA+AGG - chr4.4:289022-289041 MS.gene040115:intron 40.0%
!!! CTGATTTGCTTGAGCTTTTC+CGG - chr4.4:289249-289268 MS.gene040115:CDS 40.0%
AACAACAGGTTCCAACAAGG+AGG - chr4.4:288911-288930 MS.gene040115:intron 45.0%
ACTAGTTATCTAGCGACCAG+GGG - chr4.4:288721-288740 MS.gene040115:CDS 45.0%
AGGGTGATCATTGGACTTCA+AGG - chr4.4:289009-289028 MS.gene040115:intron 45.0%
AGTCTTTGGATCCTCCTTGT+TGG + chr4.4:288925-288944 None:intergenic 45.0%
CAAAAGACTGGCATGAGTAG+GGG - chr4.4:289311-289330 MS.gene040115:CDS 45.0%
CGGAAAAGCTCAAGCAAATC+AGG + chr4.4:289251-289270 None:intergenic 45.0%
CTTATGGTATGCAGGACTTG+CGG - chr4.4:288981-289000 MS.gene040115:intron 45.0%
GTATGCAAGAAATCCCAGGA+AGG + chr4.4:288794-288813 None:intergenic 45.0%
GTGTGATTGTGTAAATCCCC+TGG + chr4.4:288740-288759 None:intergenic 45.0%
TACATATGCACCCTTTGCTG+AGG + chr4.4:289120-289139 None:intergenic 45.0%
TTATGGTATGCAGGACTTGC+GGG - chr4.4:288982-289001 MS.gene040115:intron 45.0%
! AGACTAGTTATCTAGCGACC+AGG - chr4.4:288719-288738 MS.gene040115:CDS 45.0%
! GACTAGTTATCTAGCGACCA+GGG - chr4.4:288720-288739 MS.gene040115:CDS 45.0%
! GTGCTGTTCTTATGGTATGC+AGG - chr4.4:288973-288992 MS.gene040115:intron 45.0%
ACCCTACTGACAGCACCAAA+AGG + chr4.4:289275-289294 None:intergenic 50.0%
CTTCGGTGGAAACCATAGTG+AGG + chr4.4:288599-288618 None:intergenic 50.0%
GAAGCTAAGGTGGGGAGAAT+TGG - chr4.4:288335-288354 MS.gene040115:CDS 50.0%
GCGTTCGAGAAGGATCTCTT+CGG + chr4.4:288616-288635 None:intergenic 50.0%
GGAGAATTGGAGGAAGACGA+TGG - chr4.4:288348-288367 MS.gene040115:CDS 50.0%
GGCTGTGCGAGATCCTTATA+TGG + chr4.4:289038-289057 None:intergenic 50.0%
TACTGACAGCACCAAAAGGC+CGG + chr4.4:289271-289290 None:intergenic 50.0%
TAGCAGGGAAGATGCTCAGA+GGG - chr4.4:289355-289374 MS.gene040115:CDS 50.0%
TGTTCGGGTAACCAACCTCT+CGG - chr4.4:289211-289230 MS.gene040115:CDS 50.0%
TTACCACCACGTCAGGTGAT+AGG - chr4.4:288390-288409 MS.gene040115:CDS 50.0%
TTAGCAGGGAAGATGCTCAG+AGG - chr4.4:289354-289373 MS.gene040115:CDS 50.0%
TTCGAGAAGGATCTCTTCGG+TGG + chr4.4:288613-288632 None:intergenic 50.0%
TTGCAACAGATGCTGCTCCT+GGG - chr4.4:289084-289103 MS.gene040115:intron 50.0%
TTTCCTGTTACCACCACGTC+AGG - chr4.4:288383-288402 MS.gene040115:CDS 50.0%
! CTTGAGCTTTTCCGGCCTTT+TGG - chr4.4:289257-289276 MS.gene040115:CDS 50.0%
!!! GCCTTTTGGTGCTGTCAGTA+GGG - chr4.4:289271-289290 MS.gene040115:CDS 50.0%
ACTGGCATGAGTAGGGGCTT+TGG - chr4.4:289317-289336 MS.gene040115:CDS 55.0%
AGTCTGAACCGGCGAAGCTA+AGG - chr4.4:288322-288341 MS.gene040115:CDS 55.0%
ATGCAGGACTTGCGGGAAGA+AGG - chr4.4:288989-289008 MS.gene040115:intron 55.0%
CAACCTCTCGGAGGACACAA+GGG - chr4.4:289223-289242 MS.gene040115:CDS 55.0%
CGGTGGAAACCATAGTGAGG+CGG + chr4.4:288596-288615 None:intergenic 55.0%
CTCCGAACTTAGGCCAGGAA+CGG + chr4.4:288548-288567 None:intergenic 55.0%
GACGCCGTTCATGAAGACGT+CGG - chr4.4:288567-288586 MS.gene040115:CDS 55.0%
GAGAGTGGGTCAGCAGTCTT+TGG + chr4.4:288939-288958 None:intergenic 55.0%
GCGGGAAGAAGGGTGATCAT+TGG - chr4.4:289000-289019 MS.gene040115:intron 55.0%
GCTAAGGTGGGGAGAATTGG+AGG - chr4.4:288338-288357 MS.gene040115:CDS 55.0%
GCTTCAGCTGCCTCAGCAAA+GGG - chr4.4:289107-289126 MS.gene040115:intron 55.0%
GGAAGACGATGGCGAAGATC+TGG - chr4.4:288359-288378 MS.gene040115:CDS 55.0%
GGAGCAGCATCTGTTGCAAC+AGG + chr4.4:289083-289102 None:intergenic 55.0%
GGCGGCAATTCAGTCTGAAC+CGG - chr4.4:288311-288330 MS.gene040115:CDS 55.0%
GGTGGAAACCATAGTGAGGC+GGG + chr4.4:288595-288614 None:intergenic 55.0%
GTTGCAACAGATGCTGCTCC+TGG - chr4.4:289083-289102 MS.gene040115:intron 55.0%
TCACCTGACGTGGTGGTAAC+AGG + chr4.4:288389-288408 None:intergenic 55.0%
TCAGGACCTATCACCTGACG+TGG + chr4.4:288399-288418 None:intergenic 55.0%
TGAACCGGCGAAGCTAAGGT+GGG - chr4.4:288326-288345 MS.gene040115:CDS 55.0%
TGAACGGCGTCTCCGAACTT+AGG + chr4.4:288558-288577 None:intergenic 55.0%
TGCAACAGATGCTGCTCCTG+GGG - chr4.4:289085-289104 MS.gene040115:intron 55.0%
TGCAGGACTTGCGGGAAGAA+GGG - chr4.4:288990-289009 MS.gene040115:intron 55.0%
!! CTACCGGCTCTGACATGAGA+CGG - chr4.4:289171-289190 MS.gene040115:CDS 55.0%
!! GGCACCGACGTCTTCATGAA+CGG + chr4.4:288574-288593 None:intergenic 55.0%
!!! GGCCTTTTGGTGCTGTCAGT+AGG - chr4.4:289270-289289 MS.gene040115:CDS 55.0%
CCAACCTCTCGGAGGACACA+AGG - chr4.4:289222-289241 MS.gene040115:CDS 60.0%
CCTCCGTCTCATGTCAGAGC+CGG + chr4.4:289177-289196 None:intergenic 60.0%
CCTTGTGTCCTCCGAGAGGT+TGG + chr4.4:289225-289244 None:intergenic 60.0%
CGCCGTTCCTGGCCTAAGTT+CGG - chr4.4:288543-288562 MS.gene040115:CDS 60.0%
CTCGAACGCCCCAAACCACT+TGG - chr4.4:288627-288646 MS.gene040115:CDS 60.0%
CTGAACCGGCGAAGCTAAGG+TGG - chr4.4:288325-288344 MS.gene040115:CDS 60.0%
GAACCGGCGAAGCTAAGGTG+GGG - chr4.4:288327-288346 MS.gene040115:CDS 60.0%
GGACCTATCACCTGACGTGG+TGG + chr4.4:288396-288415 None:intergenic 60.0%
GGCAAGTTTGCGGGTTCGAG+TGG + chr4.4:288493-288512 None:intergenic 60.0%
GGCTTCAGCTGCCTCAGCAA+AGG - chr4.4:289106-289125 MS.gene040115:intron 60.0%
GGGTGGCCCATTCAACTCTC+AGG + chr4.4:289421-289440 None:intergenic 60.0%
GTACCTCCTAGCCTGAGAGC+TGG - chr4.4:289137-289156 MS.gene040115:CDS 60.0%
TCCTAGCCTGAGAGCTGGTG+CGG - chr4.4:289142-289161 MS.gene040115:CDS 60.0%
TCGGGTAACCAACCTCTCGG+AGG - chr4.4:289214-289233 MS.gene040115:CDS 60.0%
TCTCCCCACCTTAGCTTCGC+CGG + chr4.4:288333-288352 None:intergenic 60.0%
! ACGCGCGTTGGCAAGTTTGC+GGG + chr4.4:288502-288521 None:intergenic 60.0%
! GACGCGCGTTGGCAAGTTTG+CGG + chr4.4:288503-288522 None:intergenic 60.0%
! GTGGTTTGGGGCGTTCGAGA+AGG + chr4.4:288626-288645 None:intergenic 60.0%
AGGCCAGGAACGGCGCTCAA+CGG + chr4.4:288538-288557 None:intergenic 65.0%
GCACCAGCTCTCAGGCTAGG+AGG + chr4.4:289143-289162 None:intergenic 65.0%
GCTCCCTTGTGTCCTCCGAG+AGG + chr4.4:289229-289248 None:intergenic 65.0%
GCTGGTGCGGAGAGGACTAC+CGG - chr4.4:289155-289174 MS.gene040115:CDS 65.0%
GGCGTCTCCGAACTTAGGCC+AGG + chr4.4:288553-288572 None:intergenic 65.0%
TCCGCACCAGCTCTCAGGCT+AGG + chr4.4:289146-289165 None:intergenic 65.0%
TCCTCTCCGCACCAGCTCTC+AGG + chr4.4:289151-289170 None:intergenic 65.0%
! CCGGCTCTGACATGAGACGG+AGG - chr4.4:289174-289193 MS.gene040115:CDS 65.0%
! GCGTTTGCTGAGACGCGCGT+TGG + chr4.4:288514-288533 None:intergenic 65.0%
GCCTGAGAGCTGGTGCGGAG+AGG - chr4.4:289147-289166 MS.gene040115:CDS 70.0%
GCTGAGGCAGCTGAAGCCCC+AGG + chr4.4:289104-289123 None:intergenic 70.0%
! CGTCGGTGCCCGCCTCACTA+TGG - chr4.4:288584-288603 MS.gene040115:CDS 70.0%
! GCGCCGTTGAGCGCCGTTCC+TGG - chr4.4:288532-288551 MS.gene040115:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 288306 289454 288306 ID=MS.gene040115
chr4.4 mRNA 288306 289454 288306 ID=MS.gene040115.t1;Parent=MS.gene040115
chr4.4 exon 289112 289454 289112 ID=MS.gene040115.t1.exon1;Parent=MS.gene040115.t1
chr4.4 CDS 289112 289454 289112 ID=cds.MS.gene040115.t1;Parent=MS.gene040115.t1
chr4.4 exon 288306 288841 288306 ID=MS.gene040115.t1.exon2;Parent=MS.gene040115.t1
chr4.4 CDS 288306 288841 288306 ID=cds.MS.gene040115.t1;Parent=MS.gene040115.t1
Gene Sequence

>MS.gene040115

ATGGCGGCGGCAATTCAGTCTGAACCGGCGAAGCTAAGGTGGGGAGAATTGGAGGAAGACGATGGCGAAGATCTGGATTTCCTGTTACCACCACGTCAGGTGATAGGTCCTGATGAAAATGGAATCAAAAGGGTTATTGAGTACAAATTCGATGATGATGGTAATAAGGTTAAGATCACTACAACCACTCGAACCCGCAAACTTGCCAACGCGCGTCTCAGCAAACGCGCCGTTGAGCGCCGTTCCTGGCCTAAGTTCGGAGACGCCGTTCATGAAGACGTCGGTGCCCGCCTCACTATGGTTTCCACCGAAGAGATCCTTCTCGAACGCCCCAAACCACTTGGTACTTTTTCTCTTTCTCTTTCTCTCTCTTTCTAGAGATGCTACATTTCAATTTCAATTCAATTGAATCTAGACTAGTTATCTAGCGACCAGGGGATTTACACAATCACACAAGAATGTTCGGATTGAATCTATACAAAATTCCTTCCTGGGATTTCTTGCATACACATGAATTTAGCTTAGTTGTCTTAATTAATTTCACTTTCAACCTAGTCTAGTCTATTGTAGGATTTGTCTATCAATCTCATCTCATGATCTGTATCAACAACAGGTTCCAACAAGGAGGATCCAAAGACTGCTGACCCACTCTCTCAATTCAATAAAGGTGCTGTTCTTATGGTATGCAGGACTTGCGGGAAGAAGGGTGATCATTGGACTTCAAGGTGTCCATATAAGGATCTCGCACAGCCGCCTGAGACTTTTGTTGATAAACCTGTTGCAACAGATGCTGCTCCTGGGGCTTCAGCTGCCTCAGCAAAGGGTGCATATGTACCTCCTAGCCTGAGAGCTGGTGCGGAGAGGACTACCGGCTCTGACATGAGACGGAGGAACGATGAGAATTCTGTTCGGGTAACCAACCTCTCGGAGGACACAAGGGAGCCTGATTTGCTTGAGCTTTTCCGGCCTTTTGGTGCTGTCAGTAGGGTCTATGTTGCTATTGATCAAAAGACTGGCATGAGTAGGGGCTTTGGCTTTGTCAACTTTGTTAGCAGGGAAGATGCTCAGAGGGCTATCAACAAACTCAATGGATATGGCTACGACAATCTCATCCTGAGAGTTGAATGGGCCACCCCAAGAACAACCTAA
Protein sequence

>MS.gene040115.t1

MAAAIQSEPAKLRWGELEEDDGEDLDFLLPPRQVIGPDENGIKRVIEYKFDDDGNKVKITTTTRTRKLANARLSKRAVERRSWPKFGDAVHEDVGARLTMVSTEEILLERPKPLGSNKEDPKTADPLSQFNKGAVLMVCRTCGKKGDHWTSRCPYKDLAQPPETFVDKPVATDAAPGASAASAKGAYVPPSLRAGAERTTGSDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAIDQKTGMSRGFGFVNFVSREDAQRAINKLNGYGYDNLILRVEWATPRTT