AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027755

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027755.t1 MTR_5g032420 98.758 322 4 0 1 322 1 322 0.0 649
MS.gene027755.t1 MTR_8g068890 81.056 322 54 4 1 322 1 315 1.39e-177 494
MS.gene027755.t1 MTR_3g100570 74.924 327 63 7 1 322 1 313 1.31e-168 471
MS.gene027755.t1 MTR_8g086070 38.006 321 159 3 3 322 18 299 4.31e-69 218
MS.gene027755.t1 MTR_7g066690 36.825 315 168 3 6 320 17 300 1.20e-66 211
MS.gene027755.t1 MTR_4g018750 35.962 317 172 3 6 322 15 300 1.17e-64 206
MS.gene027755.t1 MTR_1g090330 32.362 309 177 3 11 318 19 296 3.88e-54 179
MS.gene027755.t1 MTR_3g462140 33.019 318 181 3 4 320 13 299 9.22e-52 173
MS.gene027755.t1 MTR_4g018750 33.779 299 163 5 6 301 15 281 6.75e-48 162
MS.gene027755.t1 MTR_4g018750 28.249 177 114 4 150 317 31 203 2.78e-14 72.4
MS.gene027755.t1 MTR_8g086070 38.356 219 96 2 3 220 18 198 1.89e-45 154
MS.gene027755.t1 MTR_1g088995 30.671 313 178 6 6 318 33 306 5.11e-41 145
MS.gene027755.t1 MTR_1g088995 29.258 229 124 5 6 234 33 223 4.17e-21 90.5
MS.gene027755.t1 MTR_4g057850 25.868 317 187 9 7 315 23 299 1.53e-19 87.8
MS.gene027755.t1 MTR_4g057850 26.087 184 129 2 127 309 15 192 1.05e-11 65.1
MS.gene027755.t1 MTR_4g012850 22.956 318 200 7 6 319 16 292 2.28e-16 78.6
MS.gene027755.t1 MTR_3g104500 23.922 255 178 7 73 320 77 322 2.59e-16 78.6
MS.gene027755.t1 MTR_5g009500 23.754 341 199 9 3 319 35 338 2.10e-15 76.3
MS.gene027755.t1 MTR_5g009500 25.510 196 137 4 131 319 31 224 5.58e-11 63.2
MS.gene027755.t1 MTR_7g117030 25.382 327 215 12 12 321 23 337 2.51e-15 75.9
MS.gene027755.t1 MTR_7g117030 26.549 226 127 7 134 322 14 237 1.47e-14 73.9
MS.gene027755.t1 MTR_2g102010 23.529 323 201 7 4 321 15 296 5.69e-15 74.3
MS.gene027755.t1 MTR_5g055410 25.987 304 184 7 22 319 37 305 8.25e-15 74.7
MS.gene027755.t1 MTR_1g087840 25.249 301 190 10 22 319 35 303 6.71e-14 71.6
MS.gene027755.t1 MTR_8g027975 23.907 343 198 12 3 319 48 353 7.23e-14 72.0
MS.gene027755.t1 MTR_8g027975 26.633 199 131 4 131 319 44 237 3.18e-12 67.0
MS.gene027755.t1 MTR_6g034515 25.246 305 171 10 21 316 82 338 1.62e-13 70.9
MS.gene027755.t1 MTR_5g073040 25.538 325 182 9 11 322 40 317 2.16e-13 70.1
MS.gene027755.t1 MTR_1g097890 27.586 232 150 5 90 319 89 304 3.56e-13 69.3
MS.gene027755.t1 MTR_4g119770 21.930 342 213 11 5 322 17 328 5.71e-13 68.9
MS.gene027755.t1 MTR_7g064980 21.277 329 217 7 3 322 22 317 6.24e-13 68.9
MS.gene027755.t1 MTR_5g030530 24.611 321 194 9 9 319 14 296 1.50e-12 67.4
MS.gene027755.t1 MTR_5g030530 24.390 205 127 5 132 319 6 199 2.78e-11 63.5
MS.gene027755.t1 MTR_8g107270 22.222 297 191 7 4 293 16 279 1.53e-12 67.8
MS.gene027755.t1 MTR_7g109135 23.824 319 200 8 8 319 24 306 1.60e-12 67.4
MS.gene027755.t1 MTR_2g098070 25.833 240 151 8 67 293 102 327 1.83e-12 67.8
MS.gene027755.t1 MTR_4g088545 26.804 194 127 5 131 316 9 195 2.58e-12 65.9
MS.gene027755.t1 MTR_4g088545 26.804 194 127 5 131 316 9 195 4.55e-12 65.9
MS.gene027755.t1 MTR_4g088545 26.702 191 131 4 131 316 9 195 5.74e-12 65.9
MS.gene027755.t1 MTR_3g028590 23.684 304 188 9 6 300 97 365 1.40e-11 65.1
MS.gene027755.t1 MTR_3g028590 23.684 304 188 9 6 300 93 361 1.47e-11 65.1
MS.gene027755.t1 MTR_7g068340 27.586 174 115 4 150 321 103 267 1.69e-11 65.1
MS.gene027755.t1 MTR_6g091800 24.038 208 128 6 128 317 12 207 4.03e-11 63.5
MS.gene027755.t1 MTR_3g098460 27.128 188 126 5 138 322 205 384 5.13e-11 63.5
MS.gene027755.t1 MTR_8g066975 25.776 322 189 10 9 319 14 296 5.98e-11 62.8
MS.gene027755.t1 MTR_8g096050 24.468 188 131 4 138 322 227 406 6.34e-11 63.5
MS.gene027755.t1 MTR_8g076760 26.908 249 162 9 85 320 65 306 6.53e-11 62.8
MS.gene027755.t1 MTR_7g083790 27.717 184 114 5 137 315 85 254 7.37e-11 62.8
MS.gene027755.t1 MTR_7g109135 23.492 315 199 8 8 319 24 299 8.63e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027755.t1 AT4G24570 73.602 322 76 4 1 322 1 313 4.43e-167 467
MS.gene027755.t1 AT2G22500 74.613 323 69 6 1 322 1 311 9.51e-163 456
MS.gene027755.t1 AT5G09470 59.531 341 111 8 1 321 1 334 3.20e-122 354
MS.gene027755.t1 AT5G19760 38.580 324 149 4 3 320 15 294 1.30e-70 221
MS.gene027755.t1 AT3G54110 36.677 319 171 4 4 322 13 300 4.20e-67 213
MS.gene027755.t1 AT5G58970 36.308 325 158 5 6 319 12 298 7.27e-66 209
MS.gene027755.t1 AT5G58970 25.455 220 114 6 9 223 127 301 2.79e-12 66.6
MS.gene027755.t1 AT1G14140 32.797 311 174 5 11 318 21 299 3.91e-52 174
MS.gene027755.t1 AT4G03115 32.075 318 170 6 3 318 35 308 1.48e-47 162
MS.gene027755.t1 AT4G03115 32.075 318 170 6 3 318 67 340 3.00e-47 162
MS.gene027755.t1 AT5G58970 35.769 260 125 4 6 254 12 240 9.87e-46 156
MS.gene027755.t1 AT5G58970 26.590 173 122 3 150 317 32 204 1.02e-16 79.3
MS.gene027755.t1 AT4G03115 33.462 260 154 5 61 318 3 245 4.63e-43 149
MS.gene027755.t1 AT4G03115 33.884 242 144 4 79 318 10 237 4.04e-41 144
MS.gene027755.t1 AT4G03115 28.879 232 124 4 3 234 35 225 5.87e-25 100
MS.gene027755.t1 AT4G03115 28.879 232 124 4 3 234 35 225 6.50e-25 100
MS.gene027755.t1 AT5G01340 26.250 320 193 8 7 320 18 300 1.27e-21 93.6
MS.gene027755.t1 AT5G01340 26.178 191 134 2 120 309 3 187 3.53e-12 66.6
MS.gene027755.t1 AT5G56450 26.820 261 163 10 73 320 81 326 5.07e-17 80.9
MS.gene027755.t1 AT5G46800 25.926 324 189 9 9 319 14 299 6.54e-17 80.1
MS.gene027755.t1 AT5G46800 22.727 198 144 2 131 319 5 202 8.34e-13 68.2
MS.gene027755.t1 AT5G46800 25.926 324 189 9 9 319 14 299 6.54e-17 80.1
MS.gene027755.t1 AT5G46800 22.727 198 144 2 131 319 5 202 8.34e-13 68.2
MS.gene027755.t1 AT5G46800 25.926 324 189 9 9 319 14 299 6.54e-17 80.1
MS.gene027755.t1 AT5G46800 22.727 198 144 2 131 319 5 202 8.34e-13 68.2
MS.gene027755.t1 AT1G25380 25.743 303 189 7 22 320 37 307 2.56e-15 76.3
MS.gene027755.t1 AT1G25380 25.743 303 189 7 22 320 37 307 2.56e-15 76.3
MS.gene027755.t1 AT1G07030 26.380 326 189 9 6 322 36 319 7.41e-15 74.7
MS.gene027755.t1 AT2G33820 25.378 331 194 12 5 319 13 306 2.75e-14 72.8
MS.gene027755.t1 AT4G01100 24.128 344 194 10 4 319 40 344 1.94e-13 70.5
MS.gene027755.t1 AT4G01100 26.396 197 134 3 131 319 35 228 3.50e-12 67.0
MS.gene027755.t1 AT3G53940 23.881 335 193 10 3 319 70 360 3.43e-13 70.1
MS.gene027755.t1 AT3G51870 30.286 175 109 5 150 321 107 271 6.53e-13 69.3
MS.gene027755.t1 AT3G51870 23.179 302 184 9 20 319 105 360 9.99e-13 68.6
MS.gene027755.t1 AT3G53940 25.389 193 127 4 138 318 73 260 6.57e-13 68.6
MS.gene027755.t1 AT4G26180 26.471 204 130 6 127 317 13 209 7.22e-13 68.6
MS.gene027755.t1 AT4G01100 25.468 267 165 7 80 319 35 294 9.49e-13 68.2
MS.gene027755.t1 AT4G26180 26.471 204 130 6 127 317 13 209 1.26e-12 68.2
MS.gene027755.t1 AT4G01100 24.058 345 209 13 3 319 39 358 1.70e-12 67.8
MS.gene027755.t1 AT4G27940 23.362 351 214 12 12 320 71 408 2.23e-12 67.8
MS.gene027755.t1 AT3G55640 24.103 195 133 3 138 321 38 228 2.68e-12 66.2
MS.gene027755.t1 AT3G55640 24.103 195 133 3 138 321 38 228 2.68e-12 66.2
MS.gene027755.t1 AT5G01500 27.528 178 118 4 146 321 131 299 3.81e-12 67.0
MS.gene027755.t1 AT5G01500 24.000 300 184 7 20 319 133 388 5.81e-12 66.6
MS.gene027755.t1 AT5G66380 28.718 195 121 8 136 322 12 196 9.99e-12 65.1
MS.gene027755.t1 AT5G17400 22.373 295 189 7 6 293 14 275 1.02e-11 65.1
MS.gene027755.t1 AT2G26360 23.364 321 182 11 3 317 112 374 1.07e-11 65.5
MS.gene027755.t1 AT2G39970 23.656 279 178 8 71 317 44 319 1.61e-11 64.7
MS.gene027755.t1 AT2G35800 23.750 320 187 9 3 320 542 806 1.80e-11 65.5
MS.gene027755.t1 AT5G51050 28.571 189 124 5 138 322 223 404 3.16e-11 64.3
MS.gene027755.t1 AT5G13490 21.959 296 190 9 6 293 88 350 3.46e-11 63.9
MS.gene027755.t1 AT5G13490 21.959 296 190 9 6 293 88 350 3.46e-11 63.9
MS.gene027755.t1 AT3G21390 23.123 333 198 11 9 313 22 324 4.54e-11 63.5
MS.gene027755.t1 AT4G28390 22.185 302 195 7 6 300 83 351 6.43e-11 63.2
MS.gene027755.t1 AT4G28390 22.185 302 195 7 6 300 83 351 6.43e-11 63.2
MS.gene027755.t1 AT3G21390 23.123 333 198 11 9 313 44 346 6.47e-11 63.2
MS.gene027755.t1 AT1G79900 23.293 249 172 5 75 319 50 283 6.70e-11 62.8
MS.gene027755.t1 AT2G47490 25.329 304 185 10 22 319 33 300 6.78e-11 62.8
MS.gene027755.t1 AT2G33820 25.389 193 133 6 130 316 14 201 7.64e-11 62.4

Find 115 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAATCCCGCCGCAAAAC+TGG 0.282254 5.2:-22442516 None:intergenic
TAAGGTTCGCATGCAACTTC+AGG 0.289431 5.2:+22441887 MS.gene027755:CDS
CACATGGATCGTGGTTGTTC+CGG 0.313068 5.2:-22441973 None:intergenic
CTCCGACCGGCACTTGCTTT+CGG 0.332288 5.2:+22441945 MS.gene027755:CDS
GGCGGGGTTGGTGCTGCTAT+TGG 0.345499 5.2:+22442230 MS.gene027755:CDS
GGACTTATTGCCGGCGGGGT+TGG 0.357971 5.2:+22442218 MS.gene027755:CDS
TGTTGTTCTGTTTGTTACTT+TGG 0.360137 5.2:+22442724 MS.gene027755:CDS
GCCACCTTATGCTGGTGCTT+TGG 0.364093 5.2:+22442613 MS.gene027755:CDS
ACATCCACTAGATCTTATTA+AGG 0.364470 5.2:+22441869 MS.gene027755:CDS
AGTTCGTGCTGAGGGTCCTT+TGG 0.375226 5.2:+22442652 MS.gene027755:CDS
GGTGTTATGCGTGATGGGCT+TGG 0.402456 5.2:+22442479 MS.gene027755:CDS
CGGAGGATCTGAACCGGGTT+CGG 0.406065 5.2:-22441927 None:intergenic
ATCGAGGCTGGACTTATTGC+CGG 0.411260 5.2:+22442209 MS.gene027755:CDS
GGGTCCTTTGGCTCTTTACA+AGG 0.417797 5.2:+22442664 MS.gene027755:CDS
GTGTTATGCGTGATGGGCTT+GGG 0.421903 5.2:+22442480 MS.gene027755:CDS
CCAACACGAGGCTGAGGAAC+CGG 0.438856 5.2:-22442005 None:intergenic
CGAACCATGGCAACATCAGC+GGG 0.440767 5.2:-22442257 None:intergenic
CTGCTTCGCCATTCTAGTAA+TGG 0.443816 5.2:-22442342 None:intergenic
GCCGGTCGGAGGATCTGAAC+CGG 0.451478 5.2:-22441933 None:intergenic
GGTGTAACAGCTTTGTTCTC+CGG 0.452972 5.2:+22442065 MS.gene027755:CDS
GCATCTCCGCCACTGTGCTC+AGG 0.455298 5.2:+22442087 MS.gene027755:CDS
CTGTTTCGCAAGATCGAGGC+TGG 0.456258 5.2:+22442197 MS.gene027755:CDS
CATTACTAGAATGGCGAAGC+AGG 0.462623 5.2:+22442343 MS.gene027755:CDS
GAGCACAGTGGCGGAGATGC+CGG 0.463595 5.2:-22442084 None:intergenic
ATCTGAACCGGGTTCGGCTT+AGG 0.465925 5.2:-22441921 None:intergenic
CGGTGGATGTTATTAAAACA+AGG 0.469643 5.2:+22442558 MS.gene027755:CDS
GGTCCCTGCCTTGAAATTGT+AGG 0.469679 5.2:-22442695 None:intergenic
GATTTATTCCTACAATTTCA+AGG 0.470932 5.2:+22442687 MS.gene027755:CDS
AGGAAGGTGTGACCAGTCTC+TGG 0.472578 5.2:+22442363 MS.gene027755:CDS
GCTGGTCACAGCTTCACAGC+TGG 0.472881 5.2:+22442418 MS.gene027755:CDS
ATAGAGTCCCATCCTGGTGG+TGG 0.474302 5.2:-22442123 None:intergenic
GGCGGCTGTTGCGTCGAATC+CGG 0.475424 5.2:+22442538 MS.gene027755:CDS
TATTCCTACAATTTCAAGGC+AGG 0.475430 5.2:+22442691 MS.gene027755:CDS
GCACTTGCTTTCGGTCAAAC+CGG 0.477758 5.2:+22441954 MS.gene027755:CDS
TGATGACCCGCGCCAGAGAC+TGG 0.484253 5.2:-22442375 None:intergenic
AAGGTTCGCATGCAACTTCA+GGG 0.489674 5.2:+22441888 MS.gene027755:CDS
GCAAGTTCGCAAGTTGTTGA+AGG 0.493746 5.2:+22442748 MS.gene027755:CDS
CAAGGAGATGATTCTTGAGA+AGG 0.493818 5.2:+22442457 MS.gene027755:CDS
AGCCGATGGGAGACTCCCAC+CGG 0.495248 5.2:+22442286 MS.gene027755:CDS
CAATCCAAAGCACCAGCATA+AGG 0.495820 5.2:-22442617 None:intergenic
ATGAACATGAAGGTGGAGGC+TGG 0.497653 5.2:+22442584 MS.gene027755:CDS
CCGCAAAACTGGCTGTCACA+TGG 0.498679 5.2:-22442505 None:intergenic
TAGTTTCTCTGCTGAGCCGG+TGG 0.518095 5.2:-22442302 None:intergenic
GGAAAAGAGCCACCTTATGC+TGG 0.522028 5.2:+22442605 MS.gene027755:CDS
AGGCTGGACTTATTGCCGGC+GGG 0.524366 5.2:+22442213 MS.gene027755:CDS
GACTGTGAACCGCGCCATGC+TGG 0.524934 5.2:+22442400 MS.gene027755:CDS
TTGAAGACAGTTCGTGCTGA+GGG 0.525849 5.2:+22442644 MS.gene027755:CDS
TTGTAGTTTCTCTGCTGAGC+CGG 0.527077 5.2:-22442305 None:intergenic
TCGAACCATGGCAACATCAG+CGG 0.533363 5.2:-22442258 None:intergenic
GTGTGACCAGTCTCTGGCGC+GGG 0.534711 5.2:+22442369 MS.gene027755:CDS
GGCTTCATATGATCAATTCA+AGG 0.534751 5.2:+22442439 MS.gene027755:CDS
CACCAGGATGGGACTCTATG+AGG 0.546356 5.2:+22442127 MS.gene027755:CDS
AGAAGGGTGTTATGCGTGAT+GGG 0.547920 5.2:+22442474 MS.gene027755:CDS
CGTGTTGGCCTTATCTCAGT+CGG 0.555631 5.2:+22442020 MS.gene027755:CDS
GAGAAGGGTGTTATGCGTGA+TGG 0.556651 5.2:+22442473 MS.gene027755:CDS
GTCCGTCTTGTTCAACAAGA+AGG 0.557738 5.2:+22442044 MS.gene027755:CDS
CACCTTCTTGTTGAACAAGA+CGG 0.561342 5.2:-22442046 None:intergenic
TGTCGACGCCATTACTAGAA+TGG 0.561691 5.2:+22442334 MS.gene027755:CDS
CCGGTTCCTCAGCCTCGTGT+TGG 0.563139 5.2:+22442005 MS.gene027755:CDS
AACCTCATAGAGTCCCATCC+TGG 0.563410 5.2:-22442129 None:intergenic
ATGGTTCGAATGCAAGCCGA+TGG 0.563779 5.2:+22442272 MS.gene027755:CDS
AAGTGGACAGATCGTGATGC+CGG 0.563971 5.2:+22442161 MS.gene027755:CDS
AAACAGCGGCATAGTGCCAC+CGG 0.564991 5.2:-22442180 None:intergenic
AAGGAGATGATTCTTGAGAA+GGG 0.566148 5.2:+22442458 MS.gene027755:CDS
GACGGACTCCGACTGAGATA+AGG 0.567027 5.2:-22442028 None:intergenic
CGCAAAACTGGCTGTCACAT+GGG 0.568264 5.2:-22442504 None:intergenic
CTGTACTCCACCACCAGGAT+GGG 0.568929 5.2:+22442116 MS.gene027755:CDS
AGTTTCTCTGCTGAGCCGGT+GGG 0.569976 5.2:-22442301 None:intergenic
ATCGGCTTGCATTCGAACCA+TGG 0.573789 5.2:-22442270 None:intergenic
AGCCGGTGGGAGTCTCCCAT+CGG 0.573898 5.2:-22442288 None:intergenic
GGTCCTTTGGCTCTTTACAA+GGG 0.576211 5.2:+22442665 MS.gene027755:CDS
TTTGAAGACAGTTCGTGCTG+AGG 0.579339 5.2:+22442643 MS.gene027755:CDS
ACAAACAGAACAACAGTGAA+AGG 0.581480 5.2:-22442716 None:intergenic
ATTCCTACAATTTCAAGGCA+GGG 0.587080 5.2:+22442692 MS.gene027755:CDS
CGAACCTTAATAAGATCTAG+TGG 0.587146 5.2:-22441873 None:intergenic
GGCTGGACTTATTGCCGGCG+GGG 0.587219 5.2:+22442214 MS.gene027755:CDS
ATAAGGCCAACACGAGGCTG+AGG 0.587508 5.2:-22442011 None:intergenic
CGAAAGCAAGTGCCGGTCGG+AGG 0.591618 5.2:-22441944 None:intergenic
AATCCCTTGTAAAGAGCCAA+AGG 0.597291 5.2:-22442668 None:intergenic
ACTGTACTCCACCACCAGGA+TGG 0.597836 5.2:+22442115 MS.gene027755:CDS
GGTATTGCTTCAATCATCGC+AGG 0.598971 5.2:+22441837 MS.gene027755:CDS
AATAAGATCTAGTGGATGTG+TGG 0.600430 5.2:-22441865 None:intergenic
CCGGTCGGAGGATCTGAACC+GGG 0.601331 5.2:-22441932 None:intergenic
CCCGGTTCAGATCCTCCGAC+CGG 0.604021 5.2:+22441932 MS.gene027755:CDS
GCCTCGATCTTGCGAAACAG+CGG 0.604552 5.2:-22442194 None:intergenic
AATAGCAGCACCAACCCCGC+CGG 0.606057 5.2:-22442228 None:intergenic
GGTGTGACCAGTCTCTGGCG+CGG 0.608726 5.2:+22442368 MS.gene027755:CDS
GCCGCTGTTTCGCAAGATCG+AGG 0.616216 5.2:+22442193 MS.gene027755:CDS
TAACCCCGCTGATGTTGCCA+TGG 0.617205 5.2:+22442253 MS.gene027755:CDS
GTTTGACCGAAAGCAAGTGC+CGG 0.620216 5.2:-22441951 None:intergenic
GACCGAAAGCAAGTGCCGGT+CGG 0.622497 5.2:-22441947 None:intergenic
GATCCATGTGGGATCTACTC+CGG 0.624295 5.2:+22441986 MS.gene027755:CDS
AAACGCGCCTAAGCCGAACC+CGG 0.624848 5.2:+22441914 MS.gene027755:CDS
ACTAGAATGGCGAAGCAGGA+AGG 0.629489 5.2:+22442347 MS.gene027755:CDS
AACAAGGGTGATGAACATGA+AGG 0.636946 5.2:+22442574 MS.gene027755:CDS
TGGTTCGAATGCAAGCCGAT+GGG 0.641550 5.2:+22442273 MS.gene027755:CDS
GGTGGATGTTATTAAAACAA+GGG 0.642233 5.2:+22442559 MS.gene027755:CDS
TGTCTGCCTGAGCACAGTGG+CGG 0.644365 5.2:-22442093 None:intergenic
CGGAACAACCACGATCCATG+TGG 0.645591 5.2:+22441974 MS.gene027755:CDS
CTGTGAAGCTGTGACCAGCA+TGG 0.646036 5.2:-22442414 None:intergenic
TCCAAAGCACCAGCATAAGG+TGG 0.647982 5.2:-22442614 None:intergenic
AGACACTGTACTCCACCACC+AGG 0.648345 5.2:+22442111 MS.gene027755:CDS
AGTAGATCCCACATGGATCG+TGG 0.651414 5.2:-22441982 None:intergenic
GAGGCTGGACTTATTGCCGG+CGG 0.651692 5.2:+22442212 MS.gene027755:CDS
CTCATAGAGTCCCATCCTGG+TGG 0.654507 5.2:-22442126 None:intergenic
GGCTGTTGCGTCGAATCCGG+TGG 0.655060 5.2:+22442541 MS.gene027755:CDS
AAGGGTGATGAACATGAAGG+TGG 0.661744 5.2:+22442577 MS.gene027755:CDS
GAACCGGAGTAGATCCCACA+TGG 0.662096 5.2:-22441989 None:intergenic
GGTGATGAACATGAAGGTGG+AGG 0.666292 5.2:+22442580 MS.gene027755:CDS
TGGACAGATCGTGATGCCGG+TGG 0.673996 5.2:+22442164 MS.gene027755:CDS
GGAACAACCACGATCCATGT+GGG 0.678666 5.2:+22441975 MS.gene027755:CDS
AAGCTGTGACCAGCATGGCG+CGG 0.685288 5.2:-22442409 None:intergenic
CAGTGTCTGCCTGAGCACAG+TGG 0.746160 5.2:-22442096 None:intergenic
ACTGAGATAAGGCCAACACG+AGG 0.747866 5.2:-22442017 None:intergenic
GAACCATGGCAACATCAGCG+GGG 0.762123 5.2:-22442256 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATTTATTCCTACAATTTCA+AGG + chr5.2:22442687-22442706 MS.gene027755:CDS 25.0%
ACATCCACTAGATCTTATTA+AGG + chr5.2:22441869-22441888 MS.gene027755:CDS 30.0%
GGTGGATGTTATTAAAACAA+GGG + chr5.2:22442559-22442578 MS.gene027755:CDS 30.0%
! TGTTGTTCTGTTTGTTACTT+TGG + chr5.2:22442724-22442743 MS.gene027755:CDS 30.0%
!!! CTTGTTTTAATAACATCCAC+CGG - chr5.2:22442560-22442579 None:intergenic 30.0%
AAGGAGATGATTCTTGAGAA+GGG + chr5.2:22442458-22442477 MS.gene027755:CDS 35.0%
ACAAACAGAACAACAGTGAA+AGG - chr5.2:22442719-22442738 None:intergenic 35.0%
ATGAGGTTCTCAAAAACAAG+TGG + chr5.2:22442144-22442163 MS.gene027755:CDS 35.0%
ATTCCTACAATTTCAAGGCA+GGG + chr5.2:22442692-22442711 MS.gene027755:CDS 35.0%
CGAACCTTAATAAGATCTAG+TGG - chr5.2:22441876-22441895 None:intergenic 35.0%
CGGTGGATGTTATTAAAACA+AGG + chr5.2:22442558-22442577 MS.gene027755:CDS 35.0%
GGCTTCATATGATCAATTCA+AGG + chr5.2:22442439-22442458 MS.gene027755:CDS 35.0%
TATTCCTACAATTTCAAGGC+AGG + chr5.2:22442691-22442710 MS.gene027755:CDS 35.0%
! AATAAGATCTAGTGGATGTG+TGG - chr5.2:22441868-22441887 None:intergenic 35.0%
AACAAGGGTGATGAACATGA+AGG + chr5.2:22442574-22442593 MS.gene027755:CDS 40.0%
AATCCCTTGTAAAGAGCCAA+AGG - chr5.2:22442671-22442690 None:intergenic 40.0%
CAAGGAGATGATTCTTGAGA+AGG + chr5.2:22442457-22442476 MS.gene027755:CDS 40.0%
CACCTTCTTGTTGAACAAGA+CGG - chr5.2:22442049-22442068 None:intergenic 40.0%
!!! GGTGTCAAAGGTTTTGTTGA+AGG + chr5.2:22441813-22441832 MS.gene027755:CDS 40.0%
!!! GTCAAAGGTTTTGTTGAAGG+TGG + chr5.2:22441816-22441835 MS.gene027755:CDS 40.0%
AAGGGTGATGAACATGAAGG+TGG + chr5.2:22442577-22442596 MS.gene027755:CDS 45.0%
AAGGTTCGCATGCAACTTCA+GGG + chr5.2:22441888-22441907 MS.gene027755:CDS 45.0%
CATTACTAGAATGGCGAAGC+AGG + chr5.2:22442343-22442362 MS.gene027755:CDS 45.0%
CTGCTTCGCCATTCTAGTAA+TGG - chr5.2:22442345-22442364 None:intergenic 45.0%
GCAAGTTCGCAAGTTGTTGA+AGG + chr5.2:22442748-22442767 MS.gene027755:CDS 45.0%
GGTATTGCTTCAATCATCGC+AGG + chr5.2:22441837-22441856 MS.gene027755:CDS 45.0%
TAAGGTTCGCATGCAACTTC+AGG + chr5.2:22441887-22441906 MS.gene027755:CDS 45.0%
TGTCGACGCCATTACTAGAA+TGG + chr5.2:22442334-22442353 MS.gene027755:CDS 45.0%
TTGAAGACAGTTCGTGCTGA+GGG + chr5.2:22442644-22442663 MS.gene027755:CDS 45.0%
TTGTAGTTTCTCTGCTGAGC+CGG - chr5.2:22442308-22442327 None:intergenic 45.0%
TTTGAAGACAGTTCGTGCTG+AGG + chr5.2:22442643-22442662 MS.gene027755:CDS 45.0%
! CAATCCAAAGCACCAGCATA+AGG - chr5.2:22442620-22442639 None:intergenic 45.0%
! GTCCGTCTTGTTCAACAAGA+AGG + chr5.2:22442044-22442063 MS.gene027755:CDS 45.0%
!! AGAAGGGTGTTATGCGTGAT+GGG + chr5.2:22442474-22442493 MS.gene027755:CDS 45.0%
!! GGTCCTTTGGCTCTTTACAA+GGG + chr5.2:22442665-22442684 MS.gene027755:CDS 45.0%
!! GGTGTAACAGCTTTGTTCTC+CGG + chr5.2:22442065-22442084 MS.gene027755:CDS 45.0%
AACCTCATAGAGTCCCATCC+TGG - chr5.2:22442132-22442151 None:intergenic 50.0%
AAGTGGACAGATCGTGATGC+CGG + chr5.2:22442161-22442180 MS.gene027755:CDS 50.0%
ACTAGAATGGCGAAGCAGGA+AGG + chr5.2:22442347-22442366 MS.gene027755:CDS 50.0%
ACTGAGATAAGGCCAACACG+AGG - chr5.2:22442020-22442039 None:intergenic 50.0%
AGTAGATCCCACATGGATCG+TGG - chr5.2:22441985-22442004 None:intergenic 50.0%
ATCGAGGCTGGACTTATTGC+CGG + chr5.2:22442209-22442228 MS.gene027755:CDS 50.0%
ATGAACATGAAGGTGGAGGC+TGG + chr5.2:22442584-22442603 MS.gene027755:CDS 50.0%
ATGGTTCGAATGCAAGCCGA+TGG + chr5.2:22442272-22442291 MS.gene027755:CDS 50.0%
CACATGGATCGTGGTTGTTC+CGG - chr5.2:22441976-22441995 None:intergenic 50.0%
CGCAAAACTGGCTGTCACAT+GGG - chr5.2:22442507-22442526 None:intergenic 50.0%
GATCCATGTGGGATCTACTC+CGG + chr5.2:22441986-22442005 MS.gene027755:CDS 50.0%
GCACTTGCTTTCGGTCAAAC+CGG + chr5.2:22441954-22441973 MS.gene027755:CDS 50.0%
GGAAAAGAGCCACCTTATGC+TGG + chr5.2:22442605-22442624 MS.gene027755:CDS 50.0%
GGAACAACCACGATCCATGT+GGG + chr5.2:22441975-22441994 MS.gene027755:CDS 50.0%
GGTCCCTGCCTTGAAATTGT+AGG - chr5.2:22442698-22442717 None:intergenic 50.0%
GGTGATGAACATGAAGGTGG+AGG + chr5.2:22442580-22442599 MS.gene027755:CDS 50.0%
GTTTGACCGAAAGCAAGTGC+CGG - chr5.2:22441954-22441973 None:intergenic 50.0%
TCGAACCATGGCAACATCAG+CGG - chr5.2:22442261-22442280 None:intergenic 50.0%
TGGTTCGAATGCAAGCCGAT+GGG + chr5.2:22442273-22442292 MS.gene027755:CDS 50.0%
! CCATGTGACAGCCAGTTTTG+CGG + chr5.2:22442505-22442524 MS.gene027755:CDS 50.0%
! TCCAAAGCACCAGCATAAGG+TGG - chr5.2:22442617-22442636 None:intergenic 50.0%
!! ATCGGCTTGCATTCGAACCA+TGG - chr5.2:22442273-22442292 None:intergenic 50.0%
!! CGTGTTGGCCTTATCTCAGT+CGG + chr5.2:22442020-22442039 MS.gene027755:CDS 50.0%
!! GAGAAGGGTGTTATGCGTGA+TGG + chr5.2:22442473-22442492 MS.gene027755:CDS 50.0%
!! GGGTCCTTTGGCTCTTTACA+AGG + chr5.2:22442664-22442683 MS.gene027755:CDS 50.0%
!! GTGTTATGCGTGATGGGCTT+GGG + chr5.2:22442480-22442499 MS.gene027755:CDS 50.0%
AAACAGCGGCATAGTGCCAC+CGG - chr5.2:22442183-22442202 None:intergenic 55.0%
ACTGTACTCCACCACCAGGA+TGG + chr5.2:22442115-22442134 MS.gene027755:CDS 55.0%
AGACACTGTACTCCACCACC+AGG + chr5.2:22442111-22442130 MS.gene027755:CDS 55.0%
AGTTCGTGCTGAGGGTCCTT+TGG + chr5.2:22442652-22442671 MS.gene027755:CDS 55.0%
AGTTTCTCTGCTGAGCCGGT+GGG - chr5.2:22442304-22442323 None:intergenic 55.0%
ATAAGGCCAACACGAGGCTG+AGG - chr5.2:22442014-22442033 None:intergenic 55.0%
ATAGAGTCCCATCCTGGTGG+TGG - chr5.2:22442126-22442145 None:intergenic 55.0%
ATCTGAACCGGGTTCGGCTT+AGG - chr5.2:22441924-22441943 None:intergenic 55.0%
CACAAATCCCGCCGCAAAAC+TGG - chr5.2:22442519-22442538 None:intergenic 55.0%
CACCAGGATGGGACTCTATG+AGG + chr5.2:22442127-22442146 MS.gene027755:CDS 55.0%
CCGCAAAACTGGCTGTCACA+TGG - chr5.2:22442508-22442527 None:intergenic 55.0%
CGAACCATGGCAACATCAGC+GGG - chr5.2:22442260-22442279 None:intergenic 55.0%
CGGAACAACCACGATCCATG+TGG + chr5.2:22441974-22441993 MS.gene027755:CDS 55.0%
CTCATAGAGTCCCATCCTGG+TGG - chr5.2:22442129-22442148 None:intergenic 55.0%
CTGTACTCCACCACCAGGAT+GGG + chr5.2:22442116-22442135 MS.gene027755:CDS 55.0%
CTGTGAAGCTGTGACCAGCA+TGG - chr5.2:22442417-22442436 None:intergenic 55.0%
CTGTTTCGCAAGATCGAGGC+TGG + chr5.2:22442197-22442216 MS.gene027755:CDS 55.0%
GAACCATGGCAACATCAGCG+GGG - chr5.2:22442259-22442278 None:intergenic 55.0%
GAACCGGAGTAGATCCCACA+TGG - chr5.2:22441992-22442011 None:intergenic 55.0%
GCCACCTTATGCTGGTGCTT+TGG + chr5.2:22442613-22442632 MS.gene027755:CDS 55.0%
GCCTCGATCTTGCGAAACAG+CGG - chr5.2:22442197-22442216 None:intergenic 55.0%
TAACCCCGCTGATGTTGCCA+TGG + chr5.2:22442253-22442272 MS.gene027755:CDS 55.0%
TAGTTTCTCTGCTGAGCCGG+TGG - chr5.2:22442305-22442324 None:intergenic 55.0%
! AGGAAGGTGTGACCAGTCTC+TGG + chr5.2:22442363-22442382 MS.gene027755:CDS 55.0%
! CAGTTTTGCGGCGGGATTTG+TGG + chr5.2:22442517-22442536 MS.gene027755:CDS 55.0%
! GACGGACTCCGACTGAGATA+AGG - chr5.2:22442031-22442050 None:intergenic 55.0%
! TGTGACAGCCAGTTTTGCGG+CGG + chr5.2:22442508-22442527 MS.gene027755:CDS 55.0%
! TTTTGCGGCGGGATTTGTGG+CGG + chr5.2:22442520-22442539 MS.gene027755:CDS 55.0%
!! GGTGTTATGCGTGATGGGCT+TGG + chr5.2:22442479-22442498 MS.gene027755:CDS 55.0%
AAACGCGCCTAAGCCGAACC+CGG + chr5.2:22441914-22441933 MS.gene027755:CDS 60.0%
AAGCTGTGACCAGCATGGCG+CGG - chr5.2:22442412-22442431 None:intergenic 60.0%
AATAGCAGCACCAACCCCGC+CGG - chr5.2:22442231-22442250 None:intergenic 60.0%
AGGCTGGACTTATTGCCGGC+GGG + chr5.2:22442213-22442232 MS.gene027755:CDS 60.0%
CAGTGTCTGCCTGAGCACAG+TGG - chr5.2:22442099-22442118 None:intergenic 60.0%
CCAACACGAGGCTGAGGAAC+CGG - chr5.2:22442008-22442027 None:intergenic 60.0%
CGGAGGATCTGAACCGGGTT+CGG - chr5.2:22441930-22441949 None:intergenic 60.0%
CTCCGACCGGCACTTGCTTT+CGG + chr5.2:22441945-22441964 MS.gene027755:CDS 60.0%
GAGGCTGGACTTATTGCCGG+CGG + chr5.2:22442212-22442231 MS.gene027755:CDS 60.0%
GCTGGTCACAGCTTCACAGC+TGG + chr5.2:22442418-22442437 MS.gene027755:CDS 60.0%
TGGACAGATCGTGATGCCGG+TGG + chr5.2:22442164-22442183 MS.gene027755:CDS 60.0%
TGTCTGCCTGAGCACAGTGG+CGG - chr5.2:22442096-22442115 None:intergenic 60.0%
! GCCGCTGTTTCGCAAGATCG+AGG + chr5.2:22442193-22442212 MS.gene027755:CDS 60.0%
! GTGACAGCCAGTTTTGCGGC+GGG + chr5.2:22442509-22442528 MS.gene027755:CDS 60.0%
!! GACCGAAAGCAAGTGCCGGT+CGG - chr5.2:22441950-22441969 None:intergenic 60.0%
AGCCGATGGGAGACTCCCAC+CGG + chr5.2:22442286-22442305 MS.gene027755:CDS 65.0%
AGCCGGTGGGAGTCTCCCAT+CGG - chr5.2:22442291-22442310 None:intergenic 65.0%
CCCGGTTCAGATCCTCCGAC+CGG + chr5.2:22441932-22441951 MS.gene027755:CDS 65.0%
CCGGTCGGAGGATCTGAACC+GGG - chr5.2:22441935-22441954 None:intergenic 65.0%
CCGGTTCCTCAGCCTCGTGT+TGG + chr5.2:22442005-22442024 MS.gene027755:CDS 65.0%
GACTGTGAACCGCGCCATGC+TGG + chr5.2:22442400-22442419 MS.gene027755:CDS 65.0%
GCATCTCCGCCACTGTGCTC+AGG + chr5.2:22442087-22442106 MS.gene027755:CDS 65.0%
GCCGGTCGGAGGATCTGAAC+CGG - chr5.2:22441936-22441955 None:intergenic 65.0%
GGACTTATTGCCGGCGGGGT+TGG + chr5.2:22442218-22442237 MS.gene027755:CDS 65.0%
GGCGGCTGTTGCGTCGAATC+CGG + chr5.2:22442538-22442557 MS.gene027755:CDS 65.0%
GGCTGGACTTATTGCCGGCG+GGG + chr5.2:22442214-22442233 MS.gene027755:CDS 65.0%
GGCTGTTGCGTCGAATCCGG+TGG + chr5.2:22442541-22442560 MS.gene027755:CDS 65.0%
GGTGTGACCAGTCTCTGGCG+CGG + chr5.2:22442368-22442387 MS.gene027755:CDS 65.0%
GTGTGACCAGTCTCTGGCGC+GGG + chr5.2:22442369-22442388 MS.gene027755:CDS 65.0%
TGATGACCCGCGCCAGAGAC+TGG - chr5.2:22442378-22442397 None:intergenic 65.0%
! GAGCACAGTGGCGGAGATGC+CGG - chr5.2:22442087-22442106 None:intergenic 65.0%
!! CGAAAGCAAGTGCCGGTCGG+AGG - chr5.2:22441947-22441966 None:intergenic 65.0%
!! GGCGGGGTTGGTGCTGCTAT+TGG + chr5.2:22442230-22442249 MS.gene027755:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 22441810 22442778 22441810 ID=MS.gene027755
chr5.2 mRNA 22441810 22442778 22441810 ID=MS.gene027755.t1;Parent=MS.gene027755
chr5.2 exon 22441810 22442778 22441810 ID=MS.gene027755.t1.exon1;Parent=MS.gene027755.t1
chr5.2 CDS 22441810 22442778 22441810 ID=cds.MS.gene027755.t1;Parent=MS.gene027755.t1
Gene Sequence

>MS.gene027755

ATGGGTGTCAAAGGTTTTGTTGAAGGTGGTATTGCTTCAATCATCGCAGGATGTTCCACACATCCACTAGATCTTATTAAGGTTCGCATGCAACTTCAGGGCGAAAACGCGCCTAAGCCGAACCCGGTTCAGATCCTCCGACCGGCACTTGCTTTCGGTCAAACCGGAACAACCACGATCCATGTGGGATCTACTCCGGTTCCTCAGCCTCGTGTTGGCCTTATCTCAGTCGGAGTCCGTCTTGTTCAACAAGAAGGTGTAACAGCTTTGTTCTCCGGCATCTCCGCCACTGTGCTCAGGCAGACACTGTACTCCACCACCAGGATGGGACTCTATGAGGTTCTCAAAAACAAGTGGACAGATCGTGATGCCGGTGGCACTATGCCGCTGTTTCGCAAGATCGAGGCTGGACTTATTGCCGGCGGGGTTGGTGCTGCTATTGGTAACCCCGCTGATGTTGCCATGGTTCGAATGCAAGCCGATGGGAGACTCCCACCGGCTCAGCAGAGAAACTACAAATCTGTTGTCGACGCCATTACTAGAATGGCGAAGCAGGAAGGTGTGACCAGTCTCTGGCGCGGGTCATCATTGACTGTGAACCGCGCCATGCTGGTCACAGCTTCACAGCTGGCTTCATATGATCAATTCAAGGAGATGATTCTTGAGAAGGGTGTTATGCGTGATGGGCTTGGGACCCATGTGACAGCCAGTTTTGCGGCGGGATTTGTGGCGGCTGTTGCGTCGAATCCGGTGGATGTTATTAAAACAAGGGTGATGAACATGAAGGTGGAGGCTGGAAAAGAGCCACCTTATGCTGGTGCTTTGGATTGTGCTTTGAAGACAGTTCGTGCTGAGGGTCCTTTGGCTCTTTACAAGGGATTTATTCCTACAATTTCAAGGCAGGGACCTTTCACTGTTGTTCTGTTTGTTACTTTGGAGCAAGTTCGCAAGTTGTTGAAGGATTTCTGA
Protein sequence

>MS.gene027755.t1

MGVKGFVEGGIASIIAGCSTHPLDLIKVRMQLQGENAPKPNPVQILRPALAFGQTGTTTIHVGSTPVPQPRVGLISVGVRLVQQEGVTALFSGISATVLRQTLYSTTRMGLYEVLKNKWTDRDAGGTMPLFRKIEAGLIAGGVGAAIGNPADVAMVRMQADGRLPPAQQRNYKSVVDAITRMAKQEGVTSLWRGSSLTVNRAMLVTASQLASYDQFKEMILEKGVMRDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEAGKEPPYAGALDCALKTVRAEGPLALYKGFIPTISRQGPFTVVLFVTLEQVRKLLKDF