AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028158


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028158.t1 MTR_1g109630 46.429 196 101 1 26 221 13 204 1.29e-62 194
MS.gene028158.t1 MTR_8g099060 35.821 201 129 0 21 221 58 258 9.56e-51 166
MS.gene028158.t1 MTR_3g449880 37.949 195 121 0 9 203 31 225 1.01e-48 160
MS.gene028158.t1 MTR_5g090340 40.500 200 111 2 21 215 87 283 4.44e-48 159
MS.gene028158.t1 MTR_5g090340 40.609 197 115 1 21 215 90 286 4.80e-48 159
MS.gene028158.t1 MTR_8g028450 37.745 204 123 1 21 220 62 265 3.46e-46 154
MS.gene028158.t1 MTR_1g099500 32.110 218 146 1 4 219 32 249 3.55e-43 146
MS.gene028158.t1 MTR_3g449880 37.952 166 103 0 38 203 38 203 2.83e-38 132
MS.gene028158.t1 MTR_3g062910 26.009 223 149 2 3 211 17 237 2.95e-32 117
MS.gene028158.t1 MTR_4g014700 28.502 207 148 0 17 223 3 209 1.16e-27 104
MS.gene028158.t1 MTR_5g018250 30.303 198 127 2 33 222 29 223 2.13e-22 91.3
MS.gene028158.t1 MTR_1g107445 29.208 202 143 0 20 221 1 202 2.14e-22 90.9
MS.gene028158.t1 MTR_6g012460 29.730 185 130 0 37 221 13 197 8.07e-21 86.7
MS.gene028158.t1 MTR_5g018080 26.812 138 101 0 40 177 18 155 1.42e-15 71.6
MS.gene028158.t1 MTR_5g018190 27.397 146 106 0 41 186 284 429 1.73e-15 75.1
MS.gene028158.t1 MTR_5g018110 27.465 142 103 0 40 181 344 485 6.87e-15 73.2
MS.gene028158.t1 MTR_6g012460 31.818 132 90 0 37 168 13 144 7.48e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028158.t1 AT3G18260 48.416 221 111 2 5 223 6 225 1.57e-79 238
MS.gene028158.t1 AT2G46170 40.094 212 127 0 9 220 40 251 3.72e-53 172
MS.gene028158.t1 AT4G23630 35.821 201 129 0 21 221 73 273 2.34e-48 160
MS.gene028158.t1 AT4G23630 35.821 201 129 0 21 221 73 273 2.34e-48 160
MS.gene028158.t1 AT3G10260 31.963 219 149 0 3 221 27 245 4.96e-48 158
MS.gene028158.t1 AT3G10260 31.963 219 149 0 3 221 27 245 4.96e-48 158
MS.gene028158.t1 AT3G10260 31.963 219 149 0 3 221 47 265 9.70e-48 158
MS.gene028158.t1 AT4G11220 34.328 201 132 0 21 221 69 269 1.54e-47 157
MS.gene028158.t1 AT3G61560 37.000 200 126 0 21 220 52 251 7.15e-46 153
MS.gene028158.t1 AT3G61560 37.000 200 126 0 21 220 52 251 7.15e-46 153
MS.gene028158.t1 AT5G41600 32.195 205 135 1 21 221 52 256 2.09e-42 144
MS.gene028158.t1 AT1G64090 34.171 199 127 1 21 215 48 246 7.04e-42 142
MS.gene028158.t1 AT1G64090 31.481 216 128 2 21 216 48 263 4.06e-38 133
MS.gene028158.t1 AT4G01230 34.783 207 132 1 7 213 40 243 5.44e-37 130
MS.gene028158.t1 AT1G68230 33.175 211 135 2 13 221 7 213 8.59e-37 128
MS.gene028158.t1 AT4G01230 33.981 206 131 2 8 213 41 241 9.14e-34 121
MS.gene028158.t1 AT3G61560 40.136 147 88 0 21 167 52 198 5.35e-32 115
MS.gene028158.t1 AT2G46170 40.876 137 81 0 9 145 40 176 2.30e-29 108
MS.gene028158.t1 AT3G10915 25.822 213 157 1 9 220 12 224 3.53e-25 99.0
MS.gene028158.t1 AT3G54120 31.250 192 132 0 14 205 1 192 1.47e-24 96.7
MS.gene028158.t1 AT3G10915 26.425 193 142 0 28 220 57 249 1.74e-24 97.8
MS.gene028158.t1 AT3G19460 31.073 177 120 2 29 204 14 189 7.31e-24 94.7
MS.gene028158.t1 AT3G19460 31.073 177 120 2 29 204 14 189 7.31e-24 94.7
MS.gene028158.t1 AT3G19460 31.073 177 120 2 29 204 14 189 7.31e-24 94.7
MS.gene028158.t1 AT3G10915 25.701 214 157 2 9 220 12 225 1.93e-23 94.4
MS.gene028158.t1 AT2G15280 25.888 197 146 0 25 221 2 198 3.31e-23 93.2
MS.gene028158.t1 AT1G68230 34.810 158 97 2 13 168 7 160 2.74e-22 89.7
MS.gene028158.t1 AT3G19460 28.634 227 120 4 29 221 14 232 1.12e-19 84.3
MS.gene028158.t1 AT3G19460 28.634 227 120 4 29 221 14 232 1.12e-19 84.3
MS.gene028158.t1 AT3G10915 24.868 189 142 0 32 220 4 192 2.31e-19 82.8
MS.gene028158.t1 AT3G10915 25.137 183 137 0 38 220 9 191 3.37e-19 82.4
MS.gene028158.t1 AT3G10915 23.305 236 157 3 9 220 12 247 2.31e-18 81.3
MS.gene028158.t1 AT3G19460 26.941 219 118 3 37 221 6 216 1.84e-15 72.8
MS.gene028158.t1 AT3G19460 26.941 219 118 3 37 221 6 216 1.84e-15 72.8
MS.gene028158.t1 AT2G23640 23.404 188 141 1 37 221 16 203 3.66e-13 66.2

Find 0 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTTAGTATATATATTTAT+TGG + 33312:10671-10690 None:intergenic 10.0%
!! ATAAATTTAAAAATGTCATT+TGG + 33312:10157-10176 None:intergenic 10.0%
!! AGTTACAATTTAAGAATTTA+AGG + 33312:9441-9460 None:intergenic 15.0%
!! GAAAATAAGTTTAAAAAGAA+AGG + 33312:10433-10452 None:intergenic 15.0%
!! GCTATTAAAAAAATGAATTA+AGG - 33312:10550-10569 MS.gene028158:intron 15.0%
!! TTATTAATTCACAATTATGA+GGG + 33312:9857-9876 None:intergenic 15.0%
!! TTTATTAATTCACAATTATG+AGG + 33312:9858-9877 None:intergenic 15.0%
!!! TAAATTTTATTTTTCAGGAA+TGG - 33312:10204-10223 MS.gene028158:CDS 15.0%
!!! TGATTTTATTTTTGTGTATA+TGG + 33312:9414-9433 None:intergenic 15.0%
!!! TTTATCAATTTGCTATATAT+TGG - 33312:10505-10524 MS.gene028158:intron 15.0%
!! AAAAAAATGAATTAAGGAAG+AGG - 33312:10556-10575 MS.gene028158:intron 20.0%
!! ATGAATTGTTCATAATGATT+TGG - 33312:9617-9636 MS.gene028158:intron 20.0%
!! CCTGAAAAATAAAATTTAAC+AGG + 33312:10202-10221 None:intergenic 20.0%
!! GTGAATTAATAAAATGTTTG+AGG - 33312:9865-9884 MS.gene028158:intron 20.0%
!! TAAACTAATTAGAATGATAG+TGG - 33312:10106-10125 MS.gene028158:intron 20.0%
!! TTAAATTGTAACTTACTCTA+TGG - 33312:9448-9467 MS.gene028158:intron 20.0%
!!! ATATGTAACAGCTTTTATTA+AGG - 33312:10064-10083 MS.gene028158:intron 20.0%
!!! CCTGTTAAATTTTATTTTTC+AGG - 33312:10199-10218 MS.gene028158:CDS 20.0%
!!! CTATCATTCTAATTAGTTTA+TGG + 33312:10106-10125 None:intergenic 20.0%
! ATTCTTGTAAAACAGTTTCT+AGG + 33312:10239-10258 None:intergenic 25.0%
! CAAATTGATAAAGTTGAAGT+AGG + 33312:10497-10516 None:intergenic 25.0%
! CACAAATACTAACACTTTAT+TGG - 33312:10375-10394 MS.gene028158:intron 25.0%
! GAAGAAACATTCTTAGTAAT+TGG + 33312:10305-10324 None:intergenic 25.0%
! GGAAAATGAAAGTTAACAAA+AGG - 33312:9353-9372 MS.gene028158:intron 25.0%
! TATGATACCAGAAGAAATAT+TGG + 33312:10351-10370 None:intergenic 25.0%
! TATTGAGGCAAATAAGATAA+TGG + 33312:9987-10006 None:intergenic 25.0%
!! ATTAATTTGGTTCCTATTTG+AGG - 33312:9716-9735 MS.gene028158:CDS 25.0%
!!! AAAATGTCATTTGGTTTGTA+AGG + 33312:10148-10167 None:intergenic 25.0%
!!! TTCATGCTATTTTTGTTTGT+GGG + 33312:9333-9352 None:intergenic 25.0%
AAACCTTATCAACCATAAAG+AGG + 33312:10696-10715 None:intergenic 30.0%
AATTGTGTATGATCGATATG+AGG - 33312:10737-10756 MS.gene028158:intron 30.0%
AATTTCATCATCAGAATCAG+AGG + 33312:9174-9193 None:intergenic 30.0%
ATACCAGAAGAAATATTGGT+AGG + 33312:10347-10366 None:intergenic 30.0%
ATACTAAGTTGTCCTCTTTA+TGG - 33312:10681-10700 MS.gene028158:intron 30.0%
ATCTGAGGAAGAAATATAGA+AGG - 33312:10796-10815 MS.gene028158:CDS 30.0%
CAATCACTGACAATATATAG+AGG + 33312:10471-10490 None:intergenic 30.0%
CACAAACAAAAATAGCATGA+AGG - 33312:9332-9351 MS.gene028158:intron 30.0%
CTCTATATATTGTCAGTGAT+TGG - 33312:10469-10488 MS.gene028158:intron 30.0%
GCCAAAAAGTAATTATAGTC+CGG - 33312:9919-9938 MS.gene028158:intron 30.0%
GTGTTCAAAATGACTTATTG+AGG + 33312:10002-10021 None:intergenic 30.0%
TCATATCGATCATACACAAT+TGG + 33312:10738-10757 None:intergenic 30.0%
TGAGGAAGAAATTAACAATC+TGG - 33312:10755-10774 MS.gene028158:intron 30.0%
TGCTTGGGATAACATTAATT+TGG - 33312:9703-9722 MS.gene028158:CDS 30.0%
TTTAACAGGCAAAGAAAATG+TGG + 33312:10188-10207 None:intergenic 30.0%
! CAAATAAGATAATGGTAGGA+TGG + 33312:9979-9998 None:intergenic 30.0%
! TATTTTCCCTTGTATTTTGC+CGG - 33312:9487-9506 MS.gene028158:intron 30.0%
! TCTGATTCTGATGATGAAAT+TGG - 33312:9173-9192 MS.gene028158:CDS 30.0%
!! TCCGGACTATAATTACTTTT+TGG + 33312:9923-9942 None:intergenic 30.0%
!! TTTGGTTGATAAATGTGTAG+TGG - 33312:9635-9654 MS.gene028158:intron 30.0%
!!! CTTCATGCTATTTTTGTTTG+TGG + 33312:9334-9353 None:intergenic 30.0%
!!! TCATGCTATTTTTGTTTGTG+GGG + 33312:9332-9351 None:intergenic 30.0%
AAGCCACTTCATGAAATTCT+TGG - 33312:9227-9246 MS.gene028158:CDS 35.0%
ACATGAGCTGATAACTTTGA+AGG - 33312:10020-10039 MS.gene028158:intron 35.0%
ATGTTTCTTCACATATCGTG+TGG - 33312:10315-10334 MS.gene028158:CDS 35.0%
ATTTGTTCCGGCAAAATACA+AGG + 33312:9497-9516 None:intergenic 35.0%
CAATGCTTGTGATATACCTA+TGG - 33312:9787-9806 MS.gene028158:CDS 35.0%
CACTTGCAGATATCTTGAAA+TGG - 33312:9814-9833 MS.gene028158:intron 35.0%
GAAGAAATTAACAATCTGGC+TGG - 33312:10759-10778 MS.gene028158:intron 35.0%
GTAAGAGAAAGTGACAAAGA+TGG + 33312:9269-9288 None:intergenic 35.0%
TATCTCAACAAAATTCCACG+AGG - 33312:10831-10850 MS.gene028158:CDS 35.0%
TGTACTCAACAACCTCAAAT+AGG + 33312:9731-9750 None:intergenic 35.0%
TTATAGTCCGGACTCAATTA+AGG - 33312:9931-9950 MS.gene028158:intron 35.0%
TTTGTTCCGGCAAAATACAA+GGG + 33312:9496-9515 None:intergenic 35.0%
! AAAACAGTTTCTAGGAACTG+CGG + 33312:10231-10250 None:intergenic 35.0%
! GAGGCAAATAAGATAATGGT+AGG + 33312:9983-10002 None:intergenic 35.0%
! GTAATTGGTTCAATCTTCTG+TGG + 33312:10290-10309 None:intergenic 35.0%
! GTGATTTTCTTTTCCTTCAC+TGG + 33312:10858-10877 None:intergenic 35.0%
! TAGGTATATCACAAGCATTG+TGG + 33312:9787-9806 None:intergenic 35.0%
! TATTTTGCCGGAACAAATGA+AGG - 33312:9499-9518 MS.gene028158:intron 35.0%
! TGTCCTCTTTATGGTTGATA+AGG - 33312:10690-10709 MS.gene028158:intron 35.0%
AGTGAGTCCTTAATTGAGTC+CGG + 33312:9941-9960 None:intergenic 40.0%
CCACCAAGAATTTCATGAAG+TGG + 33312:9233-9252 None:intergenic 40.0%
CCACTTCATGAAATTCTTGG+TGG - 33312:9230-9249 MS.gene028158:CDS 40.0%
CCTAACACCTTCATTTGTTC+CGG + 33312:9509-9528 None:intergenic 40.0%
CTTCATGAAATTCTTGGTGG+AGG - 33312:9233-9252 MS.gene028158:CDS 40.0%
GAAATTCTTGGTGGAGGAAA+AGG - 33312:9239-9258 MS.gene028158:CDS 40.0%
GACATGTCATAGTAGATCTG+AGG - 33312:10781-10800 MS.gene028158:CDS 40.0%
GCAACATATATGCATGCTAG+TGG + 33312:9901-9920 None:intergenic 40.0%
GGACCTACCAATATTTCTTC+TGG - 33312:10341-10360 MS.gene028158:CDS 40.0%
TATCTGCAAGTGTAGACCAT+AGG + 33312:9806-9825 None:intergenic 40.0%
TCTTCACATATCGTGTGGAA+CGG - 33312:10320-10339 MS.gene028158:CDS 40.0%
! TAGTGGCTGATGTGTTGTTA+TGG - 33312:9652-9671 MS.gene028158:intron 40.0%
! TATCACAAGCATTGTGGTGA+TGG + 33312:9781-9800 None:intergenic 40.0%
! TATCAGCTGCGTTTTTGCTT+GGG - 33312:9688-9707 MS.gene028158:CDS 40.0%
! TCATGAAGTGGCTTTTCATG+TGG + 33312:9221-9240 None:intergenic 40.0%
GGACAATGAAAGCAAGCTCT+TGG + 33312:10269-10288 None:intergenic 45.0%
! GGCTGATGTGTTGTTATGGA+GGG - 33312:9656-9675 MS.gene028158:intron 45.0%
! GTATCAGCTGCGTTTTTGCT+TGG - 33312:9687-9706 MS.gene028158:CDS 45.0%
! TGGCTGATGTGTTGTTATGG+AGG - 33312:9655-9674 MS.gene028158:intron 45.0%
!! CCGGAACAAATGAAGGTGTT+AGG - 33312:9506-9525 MS.gene028158:intron 45.0%
!!! ATTTTATTTATTATTTGATT+TGG - 33312:9289-9308 MS.gene028158:CDS 5.0%
AATTCCACGAGGACCAGTGA+AGG - 33312:10842-10861 MS.gene028158:CDS 50.0%
! TTTTCCTTCACTGGTCCTCG+TGG + 33312:10849-10868 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
33312 gene 9152 10881 9152 ID=MS.gene028158
33312 mRNA 9152 10881 9152 ID=MS.gene028158.t1;Parent=MS.gene028158
33312 exon 10773 10881 10773 ID=MS.gene028158.t1.exon1;Parent=MS.gene028158.t1
33312 CDS 10773 10881 10773 ID=cds.MS.gene028158.t1;Parent=MS.gene028158.t1
33312 exon 10198 10378 10198 ID=MS.gene028158.t1.exon2;Parent=MS.gene028158.t1
33312 CDS 10198 10378 10198 ID=cds.MS.gene028158.t1;Parent=MS.gene028158.t1
33312 exon 9671 9812 9671 ID=MS.gene028158.t1.exon3;Parent=MS.gene028158.t1
33312 CDS 9671 9812 9671 ID=cds.MS.gene028158.t1;Parent=MS.gene028158.t1
33312 exon 9507 9576 9507 ID=MS.gene028158.t1.exon4;Parent=MS.gene028158.t1
33312 CDS 9507 9576 9507 ID=cds.MS.gene028158.t1;Parent=MS.gene028158.t1
33312 exon 9152 9321 9152 ID=MS.gene028158.t1.exon5;Parent=MS.gene028158.t1
33312 CDS 9152 9321 9152 ID=cds.MS.gene028158.t1;Parent=MS.gene028158.t1
Gene Sequence

>MS.gene028158

ATGGCGCATCATAATACATCCTCTGATTCTGATGATGAAATTGGCAAATCTCGAGCAATGATATTTCCACATGAAAAGCCACTTCATGAAATTCTTGGTGGAGGAAAAGGTACACCATCTTTGTCACTTTCTCTTACATTTTATTTATTATTTGATTTGGACATGTAACAAAATAGTCCCCACAAACAAAAATAGCATGAAGGAAAATGAAAGTTAACAAAAGGAACACGATACATATTTGACAATGAAAATTACAAAGCCATATACACAAAAATAAAATCACTCTCCTTAAATTCTTAAATTGTAACTTACTCTATGGTAAATACTACTTAGTTTATTTTCCCTTGTATTTTGCCGGAACAAATGAAGGTGTTAGGCTTGATCGAAAAAAAGTTACTAATCGTTTTTTCACTTCTTTTCTAGCATTAAACTAATCAAATTGCTTAGATGAAATTTTGAGACTCAATGAATTGTTCATAATGATTTGGTTGATAAATGTGTAGTGGCTGATGTGTTGTTATGGAGGGACAGAAATGTATCAGCTGCGTTTTTGCTTGGGATAACATTAATTTGGTTCCTATTTGAGGTTGTTGAGTACAATATTGTGACACTTCTTTGTCACATTTCCATCACCACAATGCTTGTGATATACCTATGGTCTACACTTGCAGATATCTTGAAATGGTACACATTTTACTTTAGCCCTCATAATTGTGAATTAATAAAATGTTTGAGGTAATTTTGCTCCACTAGCATGCATATATGTTGCCAAAAAGTAATTATAGTCCGGACTCAATTAAGGACTCACTTTTGCATCAATGTTACCATCCTACCATTATCTTATTTGCCTCAATAAGTCATTTTGAACACATGAGCTGATAACTTTGAAGGAAATTTTTGTCACTTTGTGTTATATGTAACAGCTTTTATTAAGGCACAAGTAAGCTTTTTCCATAAACTAATTAGAATGATAGTGGTATATTTCTTTACTCTCCTTACAAACCAAATGACATTTTTAAATTTATCTACTTTACCACATTTTCTTTGCCTGTTAAATTTTATTTTTCAGGAATGGTCCGCAGTTCCTAGAAACTGTTTTACAAGAATCTTTCTTCCAAGAGCTTGCTTTCATTGTCCACAGAAGATTGAACCAATTACTAAGAATGTTTCTTCACATATCGTGTGGAACGGACCTACCAATATTTCTTCTGGTATCATACTATCACAAATACTAACACTTTATTGGACTCTAATTCTCAATCAATATAGTGAACTTGACCTTTCTTTTTAAACTTATTTTCACAGATCAATGTTTGCCTCTATATATTGTCAGTGATTGGAACCTACTTCAACTTTATCAATTTGCTATATATTGGTAAGAATTAACTTCTGCACATTGCTATTAAAAAAATGAATTAAGGAAGAGGAAATTACGTACAATTTTATTCTTAATTTTAGTATTTTATTATTAATTTTAGTAACTAATTCAATTTTTCTAATTGATTTGTTATTATTTCCAATAAATATATATACTAAGTTGTCCTCTTTATGGTTGATAAGGTTTTCTTTGTATACAAACACTGCCAATTGTGTATGATCGATATGAGGAAGAAATTAACAATCTGGCTGGACATGTCATAGTAGATCTGAGGAAGAAATATAGAAGGTTTAAGAAAAGATATCTCAACAAAATTCCACGAGGACCAGTGAAGGAAAAGAAAATCACATAG

Protein sequence

>MS.gene028158.t1

MAHHNTSSDSDDEIGKSRAMIFPHEKPLHEILGGGKVADVLLWRDRNVSAAFLLGITLIWFLFEVVEYNIVTLLCHISITTMLVIYLWSTLADILKWNGPQFLETVLQESFFQELAFIVHRRLNQLLRMFLHISCGTDLPIFLLINVCLYILSVIGTYFNFINLLYIGFLCIQTLPIVYDRYEEEINNLAGHVIVDLRKKYRRFKKRYLNKIPRGPVKEKKIT