AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028582

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028582.t1 MTR_4g091900 99.020 204 2 0 1 204 1 204 1.58e-146 405
MS.gene028582.t1 MTR_4g091900 98.958 192 2 0 13 204 1 192 4.28e-137 381
MS.gene028582.t1 MTR_2g081810 83.333 198 33 0 4 201 3 200 5.86e-122 343
MS.gene028582.t1 MTR_8g466030 82.323 198 35 0 4 201 3 200 7.78e-119 335
MS.gene028582.t1 MTR_2g011080 76.617 201 47 0 4 204 3 203 2.83e-112 319
MS.gene028582.t1 MTR_2g011080 75.897 195 47 0 10 204 4 198 1.20e-107 307
MS.gene028582.t1 MTR_5g070030 69.802 202 60 1 1 201 1 202 2.38e-101 291
MS.gene028582.t1 MTR_4g069250 67.005 197 64 1 5 200 52 248 1.65e-91 268
MS.gene028582.t1 MTR_5g019620 63.725 204 73 1 1 203 49 252 1.06e-90 266
MS.gene028582.t1 MTR_2g011080 74.342 152 39 0 53 204 1 152 4.11e-80 236
MS.gene028582.t1 MTR_4g091890 90.833 120 7 1 85 204 1 116 9.08e-74 218
MS.gene028582.t1 MTR_8g466080 79.310 116 24 0 86 201 1 116 6.21e-63 191
MS.gene028582.t1 MTR_8g466110 56.452 124 28 3 53 176 1 98 9.79e-35 119
MS.gene028582.t1 MTR_8g466150 56.452 124 28 3 53 176 1 98 9.79e-35 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028582.t1 AT4G27270 85.427 199 29 0 4 202 3 201 1.04e-124 350
MS.gene028582.t1 AT5G54500 84.500 200 31 0 4 203 3 202 2.37e-124 350
MS.gene028582.t1 AT4G27270 84.737 190 29 0 13 202 1 190 2.61e-117 332
MS.gene028582.t1 AT4G27270 84.737 190 29 0 13 202 1 190 2.61e-117 332
MS.gene028582.t1 AT5G58800 65.842 202 68 1 1 201 1 202 2.11e-97 281
MS.gene028582.t1 AT5G58800 65.842 202 68 1 1 201 1 202 2.11e-97 281
MS.gene028582.t1 AT5G54500 83.750 160 26 0 4 163 3 162 6.93e-97 281
MS.gene028582.t1 AT4G27270 86.000 150 21 0 53 202 1 150 1.15e-92 268
MS.gene028582.t1 AT4G36750 66.497 197 65 1 5 200 74 270 1.68e-91 269

Find 69 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGCTTCCCGACAAGATT+TGG 0.247346 4.4:-22179776 MS.gene028582:CDS
ACCTCCGCAGGCAGAGTTTC+AGG 0.301695 4.4:+22179881 None:intergenic
CTCTGCCTGCGGAGGTCGTT+GGG 0.320468 4.4:-22179874 MS.gene028582:CDS
ATAATTGGCACATCACTCTT+TGG 0.334809 4.4:+22179836 None:intergenic
GAGGCTGATGGTTTGTTGCT+TGG 0.342120 4.4:-22179794 MS.gene028582:CDS
AGCGCATCAGGTGTAATAAT+TGG 0.347422 4.4:+22179821 None:intergenic
CATCAGGGGAAGTACTTTGC+TGG 0.357702 4.4:-22179091 MS.gene028582:CDS
TGGCGTACTCAGGCACTTGC+AGG 0.358990 4.4:-22179704 MS.gene028582:CDS
GTGCCCATTGGTTACACATT+TGG 0.364441 4.4:-22179226 MS.gene028582:CDS
CACGAGACAGCCTACCGAAT+TGG 0.374655 4.4:-22179131 MS.gene028582:CDS
GTTTGAGATGGAGAGCATAA+AGG 0.387084 4.4:-22179194 MS.gene028582:CDS
TTTGAGATGGAGAGCATAAA+GGG 0.400626 4.4:-22179193 MS.gene028582:CDS
TGCGGAGGTCGTTGGGAAGA+TGG 0.403064 4.4:-22179867 MS.gene028582:CDS
TTACACAATGTAAACTTTAG+TGG 0.417852 4.4:+22181630 None:intergenic
GGGTGGTTCCCCATATGGTT+CGG 0.423157 4.4:-22179173 MS.gene028582:CDS
GCGAAGGTTCCCGAACCATA+TGG 0.433025 4.4:+22179163 None:intergenic
ACAGCCTACCGAATTGGAAT+TGG 0.433151 4.4:-22179125 MS.gene028582:CDS
GGTGGTTCCCCATATGGTTC+GGG 0.436267 4.4:-22179172 MS.gene028582:CDS
TCAGCCTCAGGTAGCGCATC+AGG 0.442003 4.4:+22179809 None:intergenic
TCTCGTGCCGTCTCCGGCGA+AGG 0.448926 4.4:+22179147 None:intergenic
GCGGAGGTCGTTGGGAAGAT+GGG 0.453021 4.4:-22179866 MS.gene028582:CDS
AAAGCATTCCTCGATGCAAC+TGG 0.456257 4.4:-22179734 MS.gene028582:CDS
ATAAAGGGTGGTTCCCCATA+TGG 0.462146 4.4:-22179178 MS.gene028582:CDS
CCCGACAAGATTTGGAATGA+TGG 0.467275 4.4:-22179768 MS.gene028582:CDS
ATTGGTTACACATTTGGTGC+TGG 0.468517 4.4:-22179220 MS.gene028582:CDS
ATTGGCTCAAGCTTTCCATC+AGG 0.473009 4.4:-22179107 MS.gene028582:CDS
CGAAGGTTCCCGAACCATAT+GGG 0.473122 4.4:+22179164 None:intergenic
TATGGTTCGGGAACCTTCGC+CGG 0.481405 4.4:-22179160 MS.gene028582:CDS
AGGCTGTCTCGTGCCGTCTC+CGG 0.489257 4.4:+22179141 None:intergenic
CCATCATTCCAAATCTTGTC+GGG 0.494553 4.4:+22179768 None:intergenic
AGCTTGAGCCAATTCCAATT+CGG 0.496465 4.4:+22179117 None:intergenic
TTTAGTTACTATTCTATGTA+CGG 0.496466 4.4:-22180220 MS.gene028582:intron
GCCATCATTCCAAATCTTGT+CGG 0.505362 4.4:+22179767 None:intergenic
TTTAAAAGGCGCGGCTTCCG+TGG 0.512381 4.4:-22180170 MS.gene028582:CDS
ATCTTCCCAACGACCTCCGC+AGG 0.515536 4.4:+22179869 None:intergenic
ACTCTGCCTGCGGAGGTCGT+TGG 0.519015 4.4:-22179875 MS.gene028582:CDS
TCGGGAACCTTCGCCGGAGA+CGG 0.520100 4.4:-22179154 MS.gene028582:CDS
AAAGGCGCGGCTTCCGTGGA+AGG 0.522189 4.4:-22180166 MS.gene028582:CDS
TCGATGCAACTGGTGGCCTA+TGG 0.530931 4.4:-22179724 MS.gene028582:CDS
ATTGGCACATCACTCTTTGG+AGG 0.535259 4.4:+22179839 None:intergenic
GCACCAAATGTGTAACCAAT+GGG 0.537151 4.4:+22179223 None:intergenic
AGTAGAAGCAAAGCTATGGC+AGG 0.545320 4.4:-22180143 MS.gene028582:intron
AGCAACAAACCATCAGCCTC+AGG 0.546743 4.4:+22179797 None:intergenic
GCACTTGCAGGAAAGCCTGC+AGG 0.551589 4.4:-22179692 MS.gene028582:CDS
GTGCTGTAAAAGATTCCTGC+AGG 0.556948 4.4:+22179677 None:intergenic
CTTTGCTTCTACTCCTTCCA+CGG 0.562351 4.4:+22180153 None:intergenic
ATCACTCAGCTTGCTCACCA+TGG 0.564969 4.4:-22179259 MS.gene028582:CDS
AGCACCAAATGTGTAACCAA+TGG 0.574155 4.4:+22179222 None:intergenic
TGGTGCTGGTATGTTTGAGA+TGG 0.575960 4.4:-22179206 MS.gene028582:CDS
AAGGAGTAGAAGCAAAGCTA+TGG 0.577868 4.4:-22180147 MS.gene028582:CDS
TGGTGGCCTATGGCGTACTC+AGG 0.578013 4.4:-22179714 MS.gene028582:CDS
CAGCAAAGTACTTCCCCTGA+TGG 0.579078 4.4:+22179092 None:intergenic
TGGCTCAAGCTTTCCATCAG+GGG 0.583615 4.4:-22179105 MS.gene028582:CDS
TTGGCTCAAGCTTTCCATCA+GGG 0.591341 4.4:-22179106 MS.gene028582:CDS
GGCATTGCAAAGAAGCTCAA+AGG 0.592964 4.4:-22179070 MS.gene028582:CDS
CAAGTGCCTGAGTACGCCAT+AGG 0.599625 4.4:+22179708 None:intergenic
ATGGTGAGCAAGCTGAGTGA+TGG 0.600815 4.4:+22179261 None:intergenic
TACAGCACTGGTTCTCAAGG+AGG 0.602179 4.4:-22179662 MS.gene028582:CDS
TGAGCCAATTCCAATTCGGT+AGG 0.602254 4.4:+22179121 None:intergenic
GAGGTCGTTGGGAAGATGGG+AGG 0.615091 4.4:-22179863 MS.gene028582:CDS
ACCTGAAACTCTGCCTGCGG+AGG 0.615512 4.4:-22179882 MS.gene028582:CDS
GAAGGTTCCCGAACCATATG+GGG 0.617327 4.4:+22179165 None:intergenic
AGCACTGGTTCTCAAGGAGG+AGG 0.618456 4.4:-22179659 MS.gene028582:CDS
GATGCGCTACCTGAGGCTGA+TGG 0.631007 4.4:-22179806 MS.gene028582:CDS
GCATTCCTCGATGCAACTGG+TGG 0.646835 4.4:-22179731 MS.gene028582:CDS
TACACCTGATGCGCTACCTG+AGG 0.657213 4.4:-22179813 MS.gene028582:CDS
GGTACCTGAAACTCTGCCTG+CGG 0.684253 4.4:-22179885 MS.gene028582:CDS
GAGATGGAGAGCATAAAGGG+TGG 0.732219 4.4:-22179190 MS.gene028582:CDS
ATAGGCCACCAGTTGCATCG+AGG 0.761372 4.4:+22179726 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATAAACAGTTTAAATT+TGG + chr4.4:22181272-22181291 None:intergenic 10.0%
!!! AAAAAATACTGTATTTTTTT+TGG - chr4.4:22179463-22179482 MS.gene028582:intron 10.0%
!!! AAAAATACTGTATTTTTTTT+GGG - chr4.4:22179464-22179483 MS.gene028582:intron 10.0%
!!! TTATTTTGAACATTTTAATT+TGG + chr4.4:22180680-22180699 None:intergenic 10.0%
!!! TTTTTCTTTAATTAAGAAAT+GGG + chr4.4:22181116-22181135 None:intergenic 10.0%
!! ACTGTTTATATATAAGTTAT+AGG - chr4.4:22181279-22181298 MS.gene028582:intron 15.0%
!! ATAAAATAATTTATGTTGCT+TGG - chr4.4:22180356-22180375 MS.gene028582:intron 15.0%
!! ATTCTCTAATATAATTTCAT+CGG + chr4.4:22179561-22179580 None:intergenic 15.0%
!! CATTTCTTAATTAAAGAAAA+AGG - chr4.4:22181115-22181134 MS.gene028582:intron 15.0%
!! TAAAATAAGTTAATCCAAAT+AGG - chr4.4:22181371-22181390 MS.gene028582:intron 15.0%
!! TATTTGAATATATGTTTCTT+TGG - chr4.4:22180066-22180085 MS.gene028582:intron 15.0%
!! TCTTATGAAAATGTAAAATA+TGG - chr4.4:22180109-22180128 MS.gene028582:intron 15.0%
!!! AAAATACTGTATTTTTTTTG+GGG - chr4.4:22179465-22179484 MS.gene028582:intron 15.0%
!!! ATAATTTTACTTATTTCTGT+TGG - chr4.4:22179524-22179543 MS.gene028582:intron 15.0%
!!! CTTTTTCTTTAATTAAGAAA+TGG + chr4.4:22181117-22181136 None:intergenic 15.0%
!!! TTTTTTTTTTGCAAATTCAA+AGG + chr4.4:22179446-22179465 None:intergenic 15.0%
!! ACTACTAAATTGATAAAACT+GGG + chr4.4:22179247-22179266 None:intergenic 20.0%
!! TACTACTAAATTGATAAAAC+TGG + chr4.4:22179248-22179267 None:intergenic 20.0%
!! TTCATAAGAAATAATTGAGA+TGG + chr4.4:22180098-22180117 None:intergenic 20.0%
!! TTGAAATTTGAAAGAAAGAA+TGG + chr4.4:22179094-22179113 None:intergenic 20.0%
!! TTTAGTTACTATTCTATGTA+CGG - chr4.4:22180477-22180496 MS.gene028582:intron 20.0%
!!! AAATACTGTATTTTTTTTGG+GGG - chr4.4:22179466-22179485 MS.gene028582:intron 20.0%
! AAAAAAAATGTTTGAGCATG+AGG - chr4.4:22179593-22179612 MS.gene028582:intron 25.0%
! AAGAAATAATTGAGATGGTT+GGG + chr4.4:22180093-22180112 None:intergenic 25.0%
! AATATGGAAGCTTAATTGAT+TGG - chr4.4:22180125-22180144 MS.gene028582:intron 25.0%
! ACCATCTTAAAGATATCTAA+TGG + chr4.4:22180703-22180722 None:intergenic 25.0%
! AGCAAACAAGCTTAAATATT+TGG - chr4.4:22181161-22181180 MS.gene028582:intron 25.0%
! GATGATAATATTGTAAATGC+AGG + chr4.4:22180781-22180800 None:intergenic 25.0%
! TAAGAAATAATTGAGATGGT+TGG + chr4.4:22180094-22180113 None:intergenic 25.0%
! TAGCTTATATGAAAACAGTT+TGG + chr4.4:22179828-22179847 None:intergenic 25.0%
! TATAGCTTTCATAAGTCTTA+AGG + chr4.4:22180392-22180411 None:intergenic 25.0%
! TATTATCATCTGATACGTAT+AGG - chr4.4:22180791-22180810 MS.gene028582:intron 25.0%
! TCAATCAATCTTTACTTGTT+TGG - chr4.4:22180644-22180663 MS.gene028582:intron 25.0%
! TGAAATCAGAAAATCAATCA+GGG + chr4.4:22181397-22181416 None:intergenic 25.0%
! TTACACAATGTAAACTTTAG+TGG + chr4.4:22179070-22179089 None:intergenic 25.0%
! TTGAAATCAGAAAATCAATC+AGG + chr4.4:22181398-22181417 None:intergenic 25.0%
!! ACCATTAGATATCTTTAAGA+TGG - chr4.4:22180699-22180718 MS.gene028582:intron 25.0%
!! CTTTTCCTTTTTATTCAAGA+AGG - chr4.4:22179878-22179897 MS.gene028582:CDS 25.0%
!! TTTATCACTCTCTGAATTTT+TGG - chr4.4:22179196-22179215 MS.gene028582:CDS 25.0%
!!! AATACTGTATTTTTTTTGGG+GGG - chr4.4:22179467-22179486 MS.gene028582:intron 25.0%
!!! ACACTTTATCTGGTTTTTAT+TGG - chr4.4:22180296-22180315 MS.gene028582:intron 25.0%
AAACAAAACTGAGTGAGATT+CGG + chr4.4:22181087-22181106 None:intergenic 30.0%
AGAAATAATTGAGATGGTTG+GGG + chr4.4:22180092-22180111 None:intergenic 30.0%
CAGATTCATGTAACATCATT+AGG + chr4.4:22180435-22180454 None:intergenic 30.0%
CTTAGGAATAACTGTAAGAA+TGG + chr4.4:22179645-22179664 None:intergenic 30.0%
GAATAACTGTAAGAATGGAT+AGG + chr4.4:22179640-22179659 None:intergenic 30.0%
TAAGTCAATCACACTTTATC+TGG - chr4.4:22180286-22180305 MS.gene028582:intron 30.0%
TCTGTCTCAAAAAACTTCTT+AGG + chr4.4:22179662-22179681 None:intergenic 30.0%
TTAATCACAGCTTTCTTTGA+GGG - chr4.4:22179343-22179362 MS.gene028582:intron 30.0%
TTCTGATTTCAAAATTCTGC+AGG - chr4.4:22181406-22181425 MS.gene028582:intron 30.0%
TTTACAACCTTCTACTGAAA+AGG + chr4.4:22179935-22179954 None:intergenic 30.0%
! ACATACCACATTTTCTCTTT+AGG - chr4.4:22180602-22180621 MS.gene028582:intron 30.0%
! CATACCACATTTTCTCTTTA+GGG - chr4.4:22180603-22180622 MS.gene028582:intron 30.0%
! CTAGCTCAAGAGATTTTAAA+AGG - chr4.4:22180513-22180532 MS.gene028582:intron 30.0%
! TTGGTAGTAATAAGTCTTTC+TGG - chr4.4:22180577-22180596 MS.gene028582:intron 30.0%
! TTTTCAGTAGAAGGTTGTAA+AGG - chr4.4:22179934-22179953 MS.gene028582:intron 30.0%
AAAATCAATCAGGGCCTATT+TGG + chr4.4:22181388-22181407 None:intergenic 35.0%
AACAAAACTGAGTGAGATTC+GGG + chr4.4:22181086-22181105 None:intergenic 35.0%
AGTTCCCTAAAGAGAAAATG+TGG + chr4.4:22180610-22180629 None:intergenic 35.0%
ATAATTGGCACATCACTCTT+TGG + chr4.4:22180864-22180883 None:intergenic 35.0%
CGCTACCTTCTTGAATAAAA+AGG + chr4.4:22179886-22179905 None:intergenic 35.0%
CTTAATCACAGCTTTCTTTG+AGG - chr4.4:22179342-22179361 MS.gene028582:intron 35.0%
CTTATCACAATTGCAAGCAA+AGG + chr4.4:22180038-22180057 None:intergenic 35.0%
CTTTGCTTGCAATTGTGATA+AGG - chr4.4:22180036-22180055 MS.gene028582:intron 35.0%
GAATGTGATTTGAGTCTTTG+AGG - chr4.4:22179275-22179294 MS.gene028582:intron 35.0%
TATGCATCAACAGAGGAATA+TGG + chr4.4:22180238-22180257 None:intergenic 35.0%
TCATTTCTTACAACCGTGTT+CGG - chr4.4:22179706-22179725 MS.gene028582:CDS 35.0%
! TGCAATCATTCGAAAAGCAT+TGG - chr4.4:22180744-22180763 MS.gene028582:intron 35.0%
! TTTGAGATGGAGAGCATAAA+GGG - chr4.4:22181504-22181523 MS.gene028582:intron 35.0%
!! GCAATCATTCGAAAAGCATT+GGG - chr4.4:22180745-22180764 MS.gene028582:intron 35.0%
!! TCAAGAGATTTTAAAAGGCG+CGG - chr4.4:22180518-22180537 MS.gene028582:intron 35.0%
AAGGAGTAGAAGCAAAGCTA+TGG - chr4.4:22180550-22180569 MS.gene028582:intron 40.0%
ACAACCTTCTACTGAAAAGG+TGG + chr4.4:22179932-22179951 None:intergenic 40.0%
AGCACCAAATGTGTAACCAA+TGG + chr4.4:22181478-22181497 None:intergenic 40.0%
AGCGCATCAGGTGTAATAAT+TGG + chr4.4:22180879-22180898 None:intergenic 40.0%
AGCTTGAGCCAATTCCAATT+CGG + chr4.4:22181583-22181602 None:intergenic 40.0%
AGTATGCACTCTTGTCTACA+TGG + chr4.4:22179970-22179989 None:intergenic 40.0%
ATCAACAGAGGAATATGGTG+TGG + chr4.4:22180233-22180252 None:intergenic 40.0%
CAACCTTCTACTGAAAAGGT+GGG + chr4.4:22179931-22179950 None:intergenic 40.0%
CCATCATTCCAAATCTTGTC+GGG + chr4.4:22180932-22180951 None:intergenic 40.0%
GCACCAAATGTGTAACCAAT+GGG + chr4.4:22181477-22181496 None:intergenic 40.0%
GCCATCATTCCAAATCTTGT+CGG + chr4.4:22180933-22180952 None:intergenic 40.0%
GTAAGCTGTTTATCCGAACA+CGG + chr4.4:22179722-22179741 None:intergenic 40.0%
TACCACTTATGCATCAACAG+AGG + chr4.4:22180245-22180264 None:intergenic 40.0%
TACTAGCAGACAAAGCATGA+TGG + chr4.4:22180182-22180201 None:intergenic 40.0%
TCTGTTGATGCATAAGTGGT+AGG - chr4.4:22180244-22180263 MS.gene028582:intron 40.0%
TGGGCACAAAAATCATTCCA+TGG + chr4.4:22181458-22181477 None:intergenic 40.0%
TTCCTCTGTTGATGCATAAG+TGG - chr4.4:22180240-22180259 MS.gene028582:intron 40.0%
TTTCGAATGATTGCAGAGGA+GGG + chr4.4:22180739-22180758 None:intergenic 40.0%
! GGAATGATTTTTGTGCCCAT+TGG - chr4.4:22181459-22181478 MS.gene028582:intron 40.0%
! GTTTGAGATGGAGAGCATAA+AGG - chr4.4:22181503-22181522 MS.gene028582:intron 40.0%
! TGCATACTTTTGATCGAGTG+TGG - chr4.4:22179982-22180001 MS.gene028582:intron 40.0%
! TGCTTTTCGAATGATTGCAG+AGG + chr4.4:22180743-22180762 None:intergenic 40.0%
! TTTTCGAATGATTGCAGAGG+AGG + chr4.4:22180740-22180759 None:intergenic 40.0%
!! ATTGGTTACACATTTGGTGC+TGG - chr4.4:22181477-22181496 MS.gene028582:intron 40.0%
!! CATCACCAATGATGGATGAT+AGG + chr4.4:22180009-22180028 None:intergenic 40.0%
!!! CTCACTCAGTTTTGTTTCGA+AGG - chr4.4:22181090-22181109 MS.gene028582:intron 40.0%
!!! TTTTACAGCACTGGTTCTCA+AGG - chr4.4:22181032-22181051 MS.gene028582:intron 40.0%
ACAGCCTACCGAATTGGAAT+TGG - chr4.4:22181572-22181591 MS.gene028582:intron 45.0%
AGTAGAAGCAAAGCTATGGC+AGG - chr4.4:22180554-22180573 MS.gene028582:intron 45.0%
ATAAAGGGTGGTTCCCCATA+TGG - chr4.4:22181519-22181538 MS.gene028582:intron 45.0%
ATTGGCACATCACTCTTTGG+AGG + chr4.4:22180861-22180880 None:intergenic 45.0%
CTTTGCTTCTACTCCTTCCA+CGG + chr4.4:22180547-22180566 None:intergenic 45.0%
GAGTGAGATTCGGGAACTTA+CGG + chr4.4:22181077-22181096 None:intergenic 45.0%
GGAAGAGTCATCACCAATGA+TGG + chr4.4:22180017-22180036 None:intergenic 45.0%
GGCATTGCAAAGAAGCTCAA+AGG - chr4.4:22181627-22181646 MS.gene028582:intron 45.0%
GTGCTGTAAAAGATTCCTGC+AGG + chr4.4:22181023-22181042 None:intergenic 45.0%
GTGGTCCTATCATCCATCAT+TGG - chr4.4:22180001-22180020 MS.gene028582:intron 45.0%
TGAGCCAATTCCAATTCGGT+AGG + chr4.4:22181579-22181598 None:intergenic 45.0%
TTGGCTCAAGCTTTCCATCA+GGG - chr4.4:22181591-22181610 MS.gene028582:intron 45.0%
! AAAGCATTCCTCGATGCAAC+TGG - chr4.4:22180963-22180982 MS.gene028582:intron 45.0%
! AGACCCACCTTTTCAGTAGA+AGG - chr4.4:22179925-22179944 MS.gene028582:intron 45.0%
! ATTGGCTCAAGCTTTCCATC+AGG - chr4.4:22181590-22181609 MS.gene028582:intron 45.0%
! CCCGACAAGATTTGGAATGA+TGG - chr4.4:22180929-22180948 MS.gene028582:intron 45.0%
! GCAGGAATCTTTTACAGCAC+TGG - chr4.4:22181023-22181042 MS.gene028582:intron 45.0%
! GTGCCCATTGGTTACACATT+TGG - chr4.4:22181471-22181490 MS.gene028582:intron 45.0%
! TTGCAGAGGAGGGTATAACT+TGG + chr4.4:22180729-22180748 None:intergenic 45.0%
!! TGGTGCTGGTATGTTTGAGA+TGG - chr4.4:22181491-22181510 MS.gene028582:intron 45.0%
!!! ACTTATTTTAATTTATATAT+TGG + chr4.4:22181361-22181380 None:intergenic 5.0%
AGCAACAAACCATCAGCCTC+AGG + chr4.4:22180903-22180922 None:intergenic 50.0%
ATGGTGAGCAAGCTGAGTGA+TGG + chr4.4:22181439-22181458 None:intergenic 50.0%
CGAAGGTTCCCGAACCATAT+GGG + chr4.4:22181536-22181555 None:intergenic 50.0%
CTTGGCTTCCCGACAAGATT+TGG - chr4.4:22180921-22180940 MS.gene028582:intron 50.0%
GAAGCAAAGCTATGGCAGGT+TGG - chr4.4:22180558-22180577 MS.gene028582:intron 50.0%
GAAGGTTCCCGAACCATATG+GGG + chr4.4:22181535-22181554 None:intergenic 50.0%
TGGCTCAAGCTTTCCATCAG+GGG - chr4.4:22181592-22181611 MS.gene028582:intron 50.0%
! ATCACTCAGCTTGCTCACCA+TGG - chr4.4:22181438-22181457 MS.gene028582:intron 50.0%
! CAGCAAAGTACTTCCCCTGA+TGG + chr4.4:22181608-22181627 None:intergenic 50.0%
! GAGATGGAGAGCATAAAGGG+TGG - chr4.4:22181507-22181526 MS.gene028582:intron 50.0%
!! CATCAGGGGAAGTACTTTGC+TGG - chr4.4:22181606-22181625 MS.gene028582:intron 50.0%
!! GAGGCTGATGGTTTGTTGCT+TGG - chr4.4:22180903-22180922 MS.gene028582:intron 50.0%
!! TACAGCACTGGTTCTCAAGG+AGG - chr4.4:22181035-22181054 MS.gene028582:intron 50.0%
ATAGGCCACCAGTTGCATCG+AGG + chr4.4:22180974-22180993 None:intergenic 55.0%
CAAGTGCCTGAGTACGCCAT+AGG + chr4.4:22180992-22181011 None:intergenic 55.0%
CACGAGACAGCCTACCGAAT+TGG - chr4.4:22181566-22181585 MS.gene028582:intron 55.0%
GCATTCCTCGATGCAACTGG+TGG - chr4.4:22180966-22180985 MS.gene028582:intron 55.0%
GCGAAGGTTCCCGAACCATA+TGG + chr4.4:22181537-22181556 None:intergenic 55.0%
GGGTGGTTCCCCATATGGTT+CGG - chr4.4:22181524-22181543 MS.gene028582:intron 55.0%
GGTACCTGAAACTCTGCCTG+CGG - chr4.4:22180812-22180831 MS.gene028582:intron 55.0%
GGTGGTTCCCCATATGGTTC+GGG - chr4.4:22181525-22181544 MS.gene028582:intron 55.0%
TACACCTGATGCGCTACCTG+AGG - chr4.4:22180884-22180903 MS.gene028582:intron 55.0%
TCGATGCAACTGGTGGCCTA+TGG - chr4.4:22180973-22180992 MS.gene028582:intron 55.0%
! TATGGTTCGGGAACCTTCGC+CGG - chr4.4:22181537-22181556 MS.gene028582:intron 55.0%
! TTTAAAAGGCGCGGCTTCCG+TGG - chr4.4:22180527-22180546 MS.gene028582:intron 55.0%
!! AGCACTGGTTCTCAAGGAGG+AGG - chr4.4:22181038-22181057 MS.gene028582:intron 55.0%
ACCTCCGCAGGCAGAGTTTC+AGG + chr4.4:22180819-22180838 None:intergenic 60.0%
ACCTGAAACTCTGCCTGCGG+AGG - chr4.4:22180815-22180834 MS.gene028582:intron 60.0%
ATCTTCCCAACGACCTCCGC+AGG + chr4.4:22180831-22180850 None:intergenic 60.0%
GAGGTCGTTGGGAAGATGGG+AGG - chr4.4:22180834-22180853 MS.gene028582:intron 60.0%
GATGCGCTACCTGAGGCTGA+TGG - chr4.4:22180891-22180910 MS.gene028582:intron 60.0%
GCACTTGCAGGAAAGCCTGC+AGG - chr4.4:22181005-22181024 MS.gene028582:intron 60.0%
GCGGAGGTCGTTGGGAAGAT+GGG - chr4.4:22180831-22180850 MS.gene028582:intron 60.0%
TCAGCCTCAGGTAGCGCATC+AGG + chr4.4:22180891-22180910 None:intergenic 60.0%
TGCGGAGGTCGTTGGGAAGA+TGG - chr4.4:22180830-22180849 MS.gene028582:intron 60.0%
TGGCGTACTCAGGCACTTGC+AGG - chr4.4:22180993-22181012 MS.gene028582:intron 60.0%
TGGTGGCCTATGGCGTACTC+AGG - chr4.4:22180983-22181002 MS.gene028582:intron 60.0%
ACTCTGCCTGCGGAGGTCGT+TGG - chr4.4:22180822-22180841 MS.gene028582:intron 65.0%
AGGCTGTCTCGTGCCGTCTC+CGG + chr4.4:22181559-22181578 None:intergenic 65.0%
CTCTGCCTGCGGAGGTCGTT+GGG - chr4.4:22180823-22180842 MS.gene028582:intron 65.0%
TCGGGAACCTTCGCCGGAGA+CGG - chr4.4:22181543-22181562 MS.gene028582:intron 65.0%
! AAAGGCGCGGCTTCCGTGGA+AGG - chr4.4:22180531-22180550 MS.gene028582:intron 65.0%
TCTCGTGCCGTCTCCGGCGA+AGG + chr4.4:22181553-22181572 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 22179060 22181659 22179060 ID=MS.gene028582
chr4.4 mRNA 22179060 22181659 22179060 ID=MS.gene028582.t1;Parent=MS.gene028582
chr4.4 exon 22181634 22181659 22181634 ID=MS.gene028582.t1.exon1;Parent=MS.gene028582.t1
chr4.4 CDS 22181634 22181659 22181634 ID=cds.MS.gene028582.t1;Parent=MS.gene028582.t1
chr4.4 exon 22180144 22180237 22180144 ID=MS.gene028582.t1.exon2;Parent=MS.gene028582.t1
chr4.4 CDS 22180144 22180237 22180144 ID=cds.MS.gene028582.t1;Parent=MS.gene028582.t1
chr4.4 exon 22179644 22179906 22179644 ID=MS.gene028582.t1.exon3;Parent=MS.gene028582.t1
chr4.4 CDS 22179644 22179906 22179644 ID=cds.MS.gene028582.t1;Parent=MS.gene028582.t1
chr4.4 exon 22179060 22179291 22179060 ID=MS.gene028582.t1.exon4;Parent=MS.gene028582.t1
chr4.4 CDS 22179060 22179291 22179060 ID=cds.MS.gene028582.t1;Parent=MS.gene028582.t1
Gene Sequence

>MS.gene028582

ATGGCTACCACTAAAGTTTACATTGTGTAAGCCATTCTTTCTTTCAAATTTCAATTGCTTTGCTTTCTTAGACAATTCTTGATTCAAAAGCAATCTAAATTTTCAACTTTTTTTTTTTTATTGTAAATTTCATTCATTTATCACTCTCTGAATTTTTGGTTTACTCTATTCATATGCTTTCAATCCCAGTTTTATCAATTTAGTAGTATATAGATGAATGTGATTTGAGTCTTTGAGGTGATTTTCATCATTTTGAAAAAGTCAACTTTGCATGATTGTAATCTTAATCACAGCTTTCTTTGAGGGTATTTGAATTCAATCTATCTAAGTCTTTGTGTTGATTAAAAAAAAAAACAGCTTTCAGAATTTCATGCTGTTTTTCACCTTTGAATTTGCAAAAAAAAAAAAATACTGTATTTTTTTTGGGGGGTTGTTCTGTTTGTCATTGATTGATATTTGTATTGATAATTTTACTTATTTCTGTTGGAACTATGTAATCCGATGAAATTATATTAGAGAATCACAAAACTTTAAAAAAAAATGTTTGAGCATGAGGATAATAAGTTAATGTGTTTGTCCTATCCATTCTTACAGTTATTCCTAAGAAGTTTTTTGAGACAGATCTGTTTTCAGATTCTTTGACTGATCATTTCTTACAACCGTGTTCGGATAAACAGCTTACTTAGACGTTTATAGCATAAACACTCATCATATGATTATTTATATAAGCTATAAGTTATTTCGATAACAAAAGATAAGATCAAGCCAAACTGTTTTCATATAAGCTATAAGCTGTTTTCATAAGCGATGATATCTCTCTTTTCCTTTTTATTCAAGAAGGTAGCGTGATTATTTATGCTAGACTAGACCCACCTTTTCAGTAGAAGGTTGTAAAGGCAATAAATAACCATGTAGACAAGAGTGCATACTTTTGATCGAGTGTGGTCCTATCATCCATCATTGGTGATGACTCTTCCTTTGCTTGCAATTGTGATAAGGCTTTTTGTATTTGAATATATGTTTCTTTGGCCCCAACCATCTCAATTATTTCTTATGAAAATGTAAAATATGGAAGCTTAATTGATTGGTTTCTTACTTTAGAAGTTGTGCTGTCAAATGCCATCATGCTTTGTCTGCTAGTACTAAATTGCTTTTGATTGTATCATAGTGCCACACCATATTCCTCTGTTGATGCATAAGTGGTAGGTATAGTAATATTTTCTAAATAAGTCAATCACACTTTATCTGGTTTTTATTGGCTGTGATCTTTAATGTTATTAAAATAATTTAGTACTGATAAAATAATTTATGTTGCTTGGTAGTATAGTACCTTAAGACTTATGAAAGCTATACTTATTTGCTTAATGTCTATCCTAATGATGTTACATGAATCTGATAAATGAATTTATGTTGCTTGTTTAGTTACTATTCTATGTACGGACATGTTGAGAAGCTAGCTCAAGAGATTTTAAAAGGCGCGGCTTCCGTGGAAGGAGTAGAAGCAAAGCTATGGCAGGTTGGTAGTAATAAGTCTTTCTGGTTACATACCACATTTTCTCTTTAGGGAACTTGTTCTAAGTTTTTTCAATCAATCTTTACTTGTTTGGCTTTAAGTCACCAAATTAAAATGTTCAAAATAACCATTAGATATCTTTAAGATGGTTAACCAAGTTATACCCTCCTCTGCAATCATTCGAAAAGCATTGGGAAATTTTTATCCTGCATTTACAATATTATCATCTGATACGTATAGGTACCTGAAACTCTGCCTGCGGAGGTCGTTGGGAAGATGGGAGGACCTCCAAAGAGTGATGTGCCAATTATTACACCTGATGCGCTACCTGAGGCTGATGGTTTGTTGCTTGGCTTCCCGACAAGATTTGGAATGATGGCTGCTCAGTTTAAAGCATTCCTCGATGCAACTGGTGGCCTATGGCGTACTCAGGCACTTGCAGGAAAGCCTGCAGGAATCTTTTACAGCACTGGTTCTCAAGGAGGAGGACAAGAGACTACACCGTAAGTTCCCGAATCTCACTCAGTTTTGTTTCGAAGGCCCATTTCTTAATTAAAGAAAAAGGCAATTTTCTTTAATAAAAGCTCAAGCAAACAAGCTTAAATATTTGGATAAACAACTTAATTAAGCGCTTATAGCATAAACACTTATCATATAAGTGTTTGTGTATAAATCATTTCTTTACAAAAAGATAAACCAAATTTAAACTGTTTATATATAAGTTATAGGTTACTTTCATAAGCTATTTCTGTAATCTCTCTCAAACAGTCTCACAATTGCTTATACCAATATATAAATTAAAATAAGTTAATCCAAATAGGCCCTGATTGATTTTCTGATTTCAAAATTCTGCAGGTTGACATCCATCACTCAGCTTGCTCACCATGGAATGATTTTTGTGCCCATTGGTTACACATTTGGTGCTGGTATGTTTGAGATGGAGAGCATAAAGGGTGGTTCCCCATATGGTTCGGGAACCTTCGCCGGAGACGGCACGAGACAGCCTACCGAATTGGAATTGGCTCAAGCTTTCCATCAGGGGAAGTACTTTGCTGGCATTGCAAAGAAGCTCAAAGGATCTCAGTGA
Protein sequence

>MS.gene028582.t1

MATTKVYIVYYSMYGHVEKLAQEILKGAASVEGVEAKLWQVPETLPAEVVGKMGGPPKSDVPIITPDALPEADGLLLGFPTRFGMMAAQFKAFLDATGGLWRTQALAGKPAGIFYSTGSQGGGQETTPLTSITQLAHHGMIFVPIGYTFGAGMFEMESIKGGSPYGSGTFAGDGTRQPTELELAQAFHQGKYFAGIAKKLKGSQ