AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031524

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031524.t1 MTR_4g081460 93.957 513 29 1 1 511 50 562 0.0 1010
MS.gene031524.t1 MTR_6g008560 54.795 511 228 2 3 511 48 557 0.0 600
MS.gene031524.t1 MTR_5g062230 55.773 511 222 2 3 511 45 553 0.0 600
MS.gene031524.t1 MTR_5g073200 56.055 512 220 3 3 511 40 549 0.0 595
MS.gene031524.t1 MTR_3g464190 56.250 512 219 3 3 511 48 557 0.0 595
MS.gene031524.t1 MTR_4g048460 56.360 511 219 2 3 511 53 561 0.0 587
MS.gene031524.t1 MTR_5g073260 54.297 512 230 3 3 511 42 552 0.0 582
MS.gene031524.t1 MTR_6g039440 53.216 513 236 2 1 511 43 553 0.0 580
MS.gene031524.t1 MTR_5g094620 52.642 511 219 3 3 511 48 537 0.0 562
MS.gene031524.t1 MTR_4g048460 57.729 414 173 1 3 416 53 464 5.12e-170 488
MS.gene031524.t1 MTR_6g008560 55.860 401 175 1 113 511 1 401 1.24e-169 485
MS.gene031524.t1 MTR_8g007515 47.287 516 261 7 3 511 45 556 6.09e-163 474
MS.gene031524.t1 MTR_2g082010 45.841 517 270 8 1 511 56 568 2.73e-162 473
MS.gene031524.t1 MTR_2g081980 45.648 517 269 9 3 511 45 557 6.10e-160 466
MS.gene031524.t1 MTR_2g082060 44.402 518 265 10 2 511 51 553 8.70e-149 437
MS.gene031524.t1 MTR_4g019550 42.015 526 270 9 2 511 52 558 1.07e-135 404
MS.gene031524.t1 MTR_2g082050 40.039 512 244 9 2 508 3 456 4.57e-124 371
MS.gene031524.t1 MTR_4g045810 38.614 505 302 5 7 508 79 578 7.51e-117 356
MS.gene031524.t1 MTR_2g065450 38.207 513 305 6 2 510 85 589 6.77e-109 336
MS.gene031524.t1 MTR_2g064425 38.207 513 304 8 2 510 82 585 3.01e-108 334
MS.gene031524.t1 MTR_8g057020 36.935 509 309 6 6 510 88 588 6.66e-107 331
MS.gene031524.t1 MTR_2g089120 36.789 492 294 7 2 488 65 544 1.49e-106 329
MS.gene031524.t1 MTR_2g089130 36.992 492 292 7 2 488 65 543 8.18e-106 327
MS.gene031524.t1 MTR_2g064425 36.647 513 297 9 2 510 82 570 1.50e-98 309
MS.gene031524.t1 MTR_4g092010 34.151 489 306 10 9 488 60 541 1.52e-89 285
MS.gene031524.t1 MTR_7g010710 33.878 490 315 5 25 508 66 552 1.35e-88 282
MS.gene031524.t1 MTR_4g092020 35.158 475 299 5 23 491 82 553 7.35e-87 278
MS.gene031524.t1 MTR_2g064425 43.807 331 178 4 2 331 82 405 3.68e-83 265
MS.gene031524.t1 MTR_2g010960 29.480 519 348 10 1 507 6 518 2.20e-76 249
MS.gene031524.t1 MTR_2g064425 41.390 331 171 5 2 331 82 390 1.04e-73 239
MS.gene031524.t1 MTR_3g465090 30.060 499 323 12 1 484 6 493 1.16e-70 234
MS.gene031524.t1 MTR_3g063120 30.638 470 305 9 30 484 6 469 2.80e-70 233
MS.gene031524.t1 MTR_3g052120 30.584 497 323 12 1 484 6 493 4.24e-69 230
MS.gene031524.t1 MTR_3g063170 29.234 496 331 11 1 484 6 493 1.90e-68 229
MS.gene031524.t1 MTR_6g064980 28.210 514 349 9 1 501 6 512 1.67e-67 226
MS.gene031524.t1 MTR_3g063140 29.259 499 327 12 1 484 6 493 1.05e-66 224
MS.gene031524.t1 MTR_5g094530 48.848 217 75 3 124 338 2 184 3.12e-63 204
MS.gene031524.t1 MTR_6g065040 29.668 391 266 6 102 484 2 391 2.75e-56 193
MS.gene031524.t1 MTR_7g057340 49.419 172 85 1 342 511 1 172 1.46e-54 181
MS.gene031524.t1 MTR_7g057390 49.412 170 85 1 342 511 1 169 7.03e-53 177
MS.gene031524.t1 MTR_7g050990 52.201 159 63 2 148 302 15 164 1.62e-46 174
MS.gene031524.t1 MTR_3g063160 30.363 303 173 7 1 285 6 288 5.11e-38 141
MS.gene031524.t1 MTR_2g064295 27.984 511 317 18 30 502 259 756 3.11e-37 146
MS.gene031524.t1 MTR_3g058160 28.023 521 316 20 30 507 68 572 3.50e-36 142
MS.gene031524.t1 MTR_3g058160 28.023 521 316 20 30 507 272 776 8.23e-36 142
MS.gene031524.t1 MTR_3g058160 28.023 521 316 20 30 507 259 763 8.28e-36 142
MS.gene031524.t1 MTR_3g058160 28.023 521 316 20 30 507 279 783 8.44e-36 142
MS.gene031524.t1 MTR_3g058160 27.306 542 314 21 30 507 279 804 4.00e-33 134
MS.gene031524.t1 MTR_3g015080 37.059 170 71 2 342 511 1 134 6.83e-31 117
MS.gene031524.t1 MTR_2g012870 23.707 464 323 12 30 468 257 714 2.09e-27 117
MS.gene031524.t1 MTR_2g012900 24.667 450 296 15 30 442 257 700 1.25e-24 108
MS.gene031524.t1 MTR_7g011663 28.025 314 181 8 16 302 335 630 1.02e-21 99.4
MS.gene031524.t1 MTR_7g094970 26.241 282 180 7 16 270 197 477 3.05e-21 97.8
MS.gene031524.t1 MTR_5g030050 27.203 261 157 8 30 266 3 254 7.40e-19 87.8
MS.gene031524.t1 MTR_7g011770 28.276 290 179 7 30 291 245 533 5.43e-18 87.8
MS.gene031524.t1 MTR_5g081730 39.583 96 58 0 119 214 16 111 1.91e-14 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031524.t1 AT5G23960 44.314 510 276 5 6 511 40 545 1.20e-150 442
MS.gene031524.t1 AT5G23960 44.336 512 275 6 6 511 40 547 2.21e-149 439
MS.gene031524.t1 AT3G14490 40.467 514 294 6 5 511 93 601 7.84e-130 390
MS.gene031524.t1 AT1G70080 38.058 515 308 7 2 507 95 607 1.89e-121 369
MS.gene031524.t1 AT3G14540 36.170 517 319 5 2 511 89 601 1.64e-116 356
MS.gene031524.t1 AT3G14520 35.590 517 322 5 2 511 92 604 4.50e-115 352
MS.gene031524.t1 AT1G31950 36.810 489 300 4 30 511 120 606 6.29e-115 352
MS.gene031524.t1 AT1G31950 36.810 489 300 4 30 511 121 607 7.76e-115 352
MS.gene031524.t1 AT3G32030 36.965 514 310 7 6 511 97 604 4.96e-112 345
MS.gene031524.t1 AT1G33750 36.062 513 319 5 3 511 96 603 5.37e-112 345
MS.gene031524.t1 AT3G29410 36.204 511 316 6 6 511 98 603 6.72e-112 344
MS.gene031524.t1 AT5G44630 37.670 515 311 7 2 508 42 554 6.92e-112 343
MS.gene031524.t1 AT3G29410 35.458 502 315 5 14 511 2 498 5.63e-111 338
MS.gene031524.t1 AT4G16730 33.080 526 328 8 2 511 22 539 7.10e-106 327
MS.gene031524.t1 AT4G20200 35.853 516 323 5 2 511 91 604 8.86e-106 328
MS.gene031524.t1 AT4G16730 33.131 495 316 5 30 511 1 493 1.78e-103 319
MS.gene031524.t1 AT4G15870 34.825 514 312 8 2 507 96 594 8.98e-103 320
MS.gene031524.t1 AT4G20210 33.140 516 337 5 2 511 87 600 8.17e-101 315
MS.gene031524.t1 AT3G29190 33.719 519 328 7 3 511 89 601 4.60e-99 311
MS.gene031524.t1 AT4G20230 34.113 513 329 7 2 507 91 601 1.76e-98 310
MS.gene031524.t1 AT4G20230 34.113 513 329 7 2 507 103 613 1.94e-98 310
MS.gene031524.t1 AT4G13300 35.328 518 317 7 2 508 41 551 2.34e-98 308
MS.gene031524.t1 AT2G23230 35.341 498 312 8 6 495 89 584 1.08e-95 303
MS.gene031524.t1 AT4G13280 35.409 514 322 6 2 508 41 551 1.75e-95 300
MS.gene031524.t1 AT2G23230 35.141 498 308 9 6 495 89 579 9.00e-94 297
MS.gene031524.t1 AT3G14540 36.730 422 259 3 2 419 89 506 3.07e-93 293
MS.gene031524.t1 AT1G48800 33.852 514 329 7 2 507 92 602 1.40e-92 294
MS.gene031524.t1 AT1G61680 33.607 488 308 7 9 488 77 556 7.29e-92 291
MS.gene031524.t1 AT1G66020 33.855 511 330 5 3 507 86 594 1.55e-91 291
MS.gene031524.t1 AT4G16740 32.762 525 325 12 1 511 54 564 7.70e-91 288
MS.gene031524.t1 AT3G29110 33.008 512 335 6 2 507 84 593 1.32e-90 289
MS.gene031524.t1 AT1G31950 37.340 391 239 2 30 416 121 509 1.75e-90 286
MS.gene031524.t1 AT1G61680 33.402 488 302 8 9 488 77 549 7.73e-90 286
MS.gene031524.t1 AT2G24210 32.830 530 314 13 6 511 79 590 2.25e-89 285
MS.gene031524.t1 AT3G25830 31.887 530 322 12 6 511 80 594 4.17e-89 285
MS.gene031524.t1 AT3G25820 31.887 530 322 12 6 511 80 594 4.17e-89 285
MS.gene031524.t1 AT3G25810 32.677 508 313 12 25 511 98 597 4.28e-85 275
MS.gene031524.t1 AT4G13280 33.852 514 300 8 2 508 41 521 4.11e-84 270
MS.gene031524.t1 AT5G48110 38.484 343 204 6 172 511 1 339 4.45e-78 248
MS.gene031524.t1 AT1G48820 32.370 519 288 12 2 507 92 560 2.95e-74 245
MS.gene031524.t1 AT3G25820 34.916 358 209 6 6 350 80 426 6.57e-66 220
MS.gene031524.t1 AT4G16740 35.920 348 204 7 1 338 54 392 1.51e-65 218
MS.gene031524.t1 AT1G66020 36.474 329 203 4 3 327 86 412 1.34e-63 213
MS.gene031524.t1 AT4G16740 38.384 297 164 7 1 287 54 341 2.84e-56 192
MS.gene031524.t1 AT4G16740 38.384 297 164 7 1 287 54 341 2.84e-56 192
MS.gene031524.t1 AT3G25820 37.627 295 160 6 6 287 80 363 6.97e-56 192
MS.gene031524.t1 AT3G25820 37.627 295 160 6 6 287 80 363 6.97e-56 192
MS.gene031524.t1 AT1G79460 24.111 506 343 15 29 505 276 769 5.64e-29 121
MS.gene031524.t1 AT1G61120 22.649 521 363 15 16 508 273 781 3.24e-25 110
MS.gene031524.t1 AT4G02780 30.466 279 162 8 16 265 320 595 8.01e-24 106

Find 91 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCAAATTGAAATCTAATT+TGG 0.157846 4.2:+28080918 None:intergenic
AGATGAAGCATTGACAATTT+TGG 0.158357 4.2:+28079955 None:intergenic
GATGATATATATGATGTTTA+TGG 0.206526 4.2:-28080601 MS.gene031524:CDS
CACAATAATCATGTTGAAAA+TGG 0.211729 4.2:-28081762 MS.gene031524:CDS
CAAAGTCCCTTTACTCATAT+TGG 0.261866 4.2:+28079518 None:intergenic
GTCATTCTTTGTTGATCCTT+GGG 0.305154 4.2:+28079568 None:intergenic
AGAGGCTTTGGCACTTGAAT+TGG 0.307687 4.2:+28079662 None:intergenic
GATTGATTCAATCCAACGTT+TGG 0.313958 4.2:-28081835 MS.gene031524:CDS
TGTCATTCTTTGTTGATCCT+TGG 0.335528 4.2:+28079567 None:intergenic
GAATAGTTGAAGCTTACTTT+TGG 0.351708 4.2:-28080705 MS.gene031524:CDS
GCTCTTGGAATAGTAAATTC+TGG 0.365262 4.2:-28080061 MS.gene031524:CDS
TTTGTGCTTGATTACCTCAT+TGG 0.372285 4.2:+28079901 None:intergenic
ATTCAATTGCACTTGTCTTC+AGG 0.387495 4.2:-28081713 MS.gene031524:CDS
CACTTGTCTTCAGGTTACTA+AGG 0.392261 4.2:-28081704 MS.gene031524:CDS
TTTGGATGTTTATGAAGAAA+TGG 0.403778 4.2:-28080382 MS.gene031524:CDS
ATTGATTCAATCCAACGTTT+GGG 0.408878 4.2:-28081834 MS.gene031524:CDS
CAAGAAATTGCCTAGGCTAC+TGG 0.414212 4.2:-28081004 MS.gene031524:CDS
CTCAATACTCTGTTGGTAGA+AGG 0.414840 4.2:-28080660 MS.gene031524:CDS
GCATTACATTTCCACTTATG+AGG 0.423418 4.2:-28080977 MS.gene031524:CDS
GCCACACATATGAGGATTCA+TGG 0.435188 4.2:-28081144 MS.gene031524:CDS
TCAAATTAGCTTCCCTCAAA+GGG 0.439126 4.2:+28081856 None:intergenic
AACAACATCCTTCGTTGGAA+TGG 0.440069 4.2:-28080023 MS.gene031524:CDS
CCCTTTACTCATATTGGACA+TGG 0.445169 4.2:+28079524 None:intergenic
ACTATTCTGTTGCGAGCAAA+AGG 0.456530 4.2:+28080721 None:intergenic
GCCATGTACTCCTCTATTGT+TGG 0.460245 4.2:+28080082 None:intergenic
ACAACATCCTTCGTTGGAAT+GGG 0.463666 4.2:-28080022 MS.gene031524:CDS
ATTGATGTGCTTTATAAAGA+TGG 0.464933 4.2:-28079608 MS.gene031524:CDS
ATAGAGGAGTACATGGCTCT+TGG 0.467532 4.2:-28080076 MS.gene031524:CDS
CATACTTTACTGAGGCTCAA+TGG 0.470064 4.2:-28080123 MS.gene031524:CDS
AAAATGTCACAAACCCTTTG+AGG 0.477504 4.2:-28081869 MS.gene031524:CDS
AAACAACATCAGAAAGAAGT+TGG 0.478105 4.2:-28080892 MS.gene031524:CDS
TGTATGAAGCCACACATATG+AGG 0.482529 4.2:-28081152 MS.gene031524:CDS
ATCAAATTAGCTTCCCTCAA+AGG 0.485801 4.2:+28081855 None:intergenic
ACTCGCATAAGGAGAGGCTT+TGG 0.486941 4.2:+28079650 None:intergenic
ATCTAAAATTGCTAGACTCA+TGG 0.490115 4.2:-28079936 MS.gene031524:CDS
GAGCCTCAGTAAAGTATGCC+TGG 0.491242 4.2:+28080128 None:intergenic
CAAGACAAGTCACAAATGCT+TGG 0.491285 4.2:-28079712 MS.gene031524:CDS
TGCACAATAGATGAAAAGAT+TGG 0.497980 4.2:-28080152 MS.gene031524:intron
CAATGTCATGCTCAAAATGA+TGG 0.500528 4.2:+28081805 None:intergenic
AACTTCAAAGAAACACTTGT+TGG 0.503713 4.2:-28081198 MS.gene031524:CDS
TTCTTCATAAACATCCAAAA+CGG 0.505524 4.2:+28080386 None:intergenic
ATTGAGTGGTCATAATTTCA+AGG 0.509130 4.2:+28081079 None:intergenic
AGAGGGATCTTCCTCATAAG+TGG 0.512911 4.2:+28080966 None:intergenic
TAATGCCTTGCCAGTAGCCT+AGG 0.520942 4.2:+28080994 None:intergenic
AAAATATATACCAACAATAG+AGG 0.522821 4.2:-28080092 MS.gene031524:CDS
AAATGTCACAAACCCTTTGA+GGG 0.523712 4.2:-28081868 MS.gene031524:CDS
TTTGAGCCTCAATACTCTGT+TGG 0.527980 4.2:-28080667 MS.gene031524:CDS
TAGCAAAATTTCATGTATTG+TGG 0.530446 4.2:+28080414 None:intergenic
ATCATCAATTACTGAGGCCA+AGG 0.530914 4.2:+28080618 None:intergenic
ACAAATTGAGAACTCGCATA+AGG 0.532358 4.2:+28079639 None:intergenic
AAGAAGTTCAAGAATGAGCA+AGG 0.540053 4.2:-28081222 MS.gene031524:CDS
ATCACAACAACATCCTTCGT+TGG 0.542322 4.2:-28080028 MS.gene031524:CDS
ATAGATGAAAAGATTGGTCC+AGG 0.544707 4.2:-28080146 MS.gene031524:intron
ACAAGTCACAAATGCTTGGA+AGG 0.546592 4.2:-28079708 MS.gene031524:CDS
AATGACCAAGGTGATATCCT+TGG 0.547258 4.2:-28080635 MS.gene031524:CDS
TTTGAGCAAGAGAGAGGGCA+TGG 0.551451 4.2:-28079803 MS.gene031524:intron
TAGGTGGGATATAAGTTGCA+TGG 0.561499 4.2:-28080445 MS.gene031524:CDS
AGCAAAGTTGCATCATGAGA+GGG 0.572891 4.2:+28080949 None:intergenic
AATTAGTTTGAGCAAGAGAG+AGG 0.573171 4.2:-28079809 MS.gene031524:intron
GGATGATATTGTTTCCAATG+AGG 0.573519 4.2:-28079915 MS.gene031524:intron
AGACAAGTGCAATTGAATGA+AGG 0.576123 4.2:+28081718 None:intergenic
AACACTTGTTGGTGATGTTG+AGG 0.576884 4.2:-28081187 MS.gene031524:CDS
ACACTTGTTGGTGATGTTGA+GGG 0.577847 4.2:-28081186 MS.gene031524:CDS
TGAAGAAATGGAGCAAGAGA+TGG 0.580004 4.2:-28080370 MS.gene031524:CDS
ACCAACAATAGAGGAGTACA+TGG 0.580785 4.2:-28080083 MS.gene031524:CDS
TCCATGAATCCTCATATGTG+TGG 0.591257 4.2:+28081143 None:intergenic
CTAGGCAATTTCTTGAAGAG+TGG 0.592350 4.2:+28081012 None:intergenic
ATTAGTTTGAGCAAGAGAGA+GGG 0.599571 4.2:-28079808 MS.gene031524:intron
CTTCTACCAACAGAGTATTG+AGG 0.601314 4.2:+28080661 None:intergenic
TAGCAAAGTTGCATCATGAG+AGG 0.612571 4.2:+28080948 None:intergenic
CACTCTTCAAGAAATTGCCT+AGG 0.614274 4.2:-28081011 MS.gene031524:CDS
GGGATGCATTGCTACAGAAG+AGG 0.614970 4.2:-28080002 MS.gene031524:CDS
GAGCAAGAGATGGTCAAGGA+AGG 0.615004 4.2:-28080360 MS.gene031524:CDS
AATGCATCCCATTCCAACGA+AGG 0.632549 4.2:+28080015 None:intergenic
GATGGTATAATCCCAAACGT+TGG 0.633632 4.2:+28081823 None:intergenic
GGTCATAATTTCAAGGTGTG+AGG 0.634454 4.2:+28081086 None:intergenic
ACATATGAGGATTCATGGAG+AGG 0.637251 4.2:-28081139 MS.gene031524:CDS
AAATTAATCATAGCTTAAAG+CGG 0.641455 4.2:-28081035 MS.gene031524:CDS
TGAGGCCAAGGATATCACCT+TGG 0.643385 4.2:+28080630 None:intergenic
TTCAGGTTACTAAGGCAACA+AGG 0.644143 4.2:-28081696 MS.gene031524:CDS
GATTGCTATACTAACTCCCA+AGG 0.653661 4.2:-28079584 MS.gene031524:CDS
AAGAAAAGGACTCAATTGAG+TGG 0.658799 4.2:+28081065 None:intergenic
AATGGAGCAAGAGATGGTCA+AGG 0.660378 4.2:-28080364 MS.gene031524:CDS
TTGAGAACTCGCATAAGGAG+AGG 0.660485 4.2:+28079644 None:intergenic
CATGTAGCACTCAATAGCAG+AGG 0.671786 4.2:+28079778 None:intergenic
ATATATATCATCAATTACTG+AGG 0.671981 4.2:+28080612 None:intergenic
AATTGCCTAGGCTACTGGCA+AGG 0.675740 4.2:-28080999 MS.gene031524:CDS
GGTCCAGGCATACTTTACTG+AGG 0.678982 4.2:-28080131 MS.gene031524:CDS
TGCTTGGAAGGATATCAATG+AGG 0.688978 4.2:-28079696 MS.gene031524:CDS
TGGTAGAAGGATAATGACCA+AGG 0.706697 4.2:-28080647 MS.gene031524:CDS
GAGTGCTACATGAATGAACA+TGG 0.715393 4.2:-28079767 MS.gene031524:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAATTAAAATGATTAAA+TGG - chr4.2:28080157-28080176 MS.gene031524:intron 10.0%
!! TTTAATTCAAATTAATTAAG+AGG + chr4.2:28080987-28081006 None:intergenic 10.0%
!!! AATATTGAACTATTTTTTTA+TGG - chr4.2:28080950-28080969 MS.gene031524:CDS 10.0%
!!! TGATTTTTTAAAATTATTAG+TGG - chr4.2:28080022-28080041 MS.gene031524:CDS 10.0%
!!! TTAATTTGAATTAAAATTCT+AGG - chr4.2:28080992-28081011 MS.gene031524:CDS 10.0%
!!! TTTTAAAAAATCACATAATT+CGG + chr4.2:28080015-28080034 None:intergenic 10.0%
!!! AAGAATTTTTACTTCATTTT+TGG + chr4.2:28081145-28081164 None:intergenic 15.0%
!!! TTTGTTTTCTTTAATGAATT+AGG - chr4.2:28080679-28080698 MS.gene031524:CDS 15.0%
!! AAAATATATACCAACAATAG+AGG - chr4.2:28081364-28081383 MS.gene031524:intron 20.0%
!! AAATTAATCATAGCTTAAAG+CGG - chr4.2:28080421-28080440 MS.gene031524:CDS 20.0%
!! AAGCAAATTGAAATCTAATT+TGG + chr4.2:28080541-28080560 None:intergenic 20.0%
!! AATATTGAGATGTGAAAATT+TGG + chr4.2:28080936-28080955 None:intergenic 20.0%
!! ATATATATCATCAATTACTG+AGG + chr4.2:28080847-28080866 None:intergenic 20.0%
!! ATTTGAATTAAAATTCTAGG+TGG - chr4.2:28080995-28081014 MS.gene031524:CDS 20.0%
!! GATGATATATATGATGTTTA+TGG - chr4.2:28080855-28080874 MS.gene031524:intron 20.0%
!! TTTGAATTAAAATTCTAGGT+GGG - chr4.2:28080996-28081015 MS.gene031524:CDS 20.0%
!!! CATATTCTTGTAAATTGTTT+TGG - chr4.2:28081275-28081294 MS.gene031524:intron 20.0%
!!! TTAATTTTTGTAGCAAGAAA+AGG + chr4.2:28080408-28080427 None:intergenic 20.0%
! AAATCTAATACAAGTAGACA+AGG + chr4.2:28079813-28079832 None:intergenic 25.0%
! ATTGATGTGCTTTATAAAGA+TGG - chr4.2:28081848-28081867 MS.gene031524:CDS 25.0%
! CACAATAATCATGTTGAAAA+TGG - chr4.2:28079694-28079713 MS.gene031524:CDS 25.0%
! CTTAACCATATATTATCATG+TGG - chr4.2:28080196-28080215 MS.gene031524:intron 25.0%
! TAGCAAAATTTCATGTATTG+TGG + chr4.2:28081045-28081064 None:intergenic 25.0%
! TTCTTCATAAACATCCAAAA+CGG + chr4.2:28081073-28081092 None:intergenic 25.0%
! TTTGGATGTTTATGAAGAAA+TGG - chr4.2:28081074-28081093 MS.gene031524:CDS 25.0%
!! GTTTTCTTTAATGAATTAGG+TGG - chr4.2:28080682-28080701 MS.gene031524:CDS 25.0%
AAACAACATCAGAAAGAAGT+TGG - chr4.2:28080564-28080583 MS.gene031524:CDS 30.0%
AACTTCAAAGAAACACTTGT+TGG - chr4.2:28080258-28080277 MS.gene031524:intron 30.0%
ATTGAGTGGTCATAATTTCA+AGG + chr4.2:28080380-28080399 None:intergenic 30.0%
GAGAACCACATGATAATATA+TGG + chr4.2:28080204-28080223 None:intergenic 30.0%
GTTAGTGTCTGTATCATATT+CGG - chr4.2:28080054-28080073 MS.gene031524:CDS 30.0%
TAGCATATATTGATCTCCAT+CGG - chr4.2:28080102-28080121 MS.gene031524:CDS 30.0%
TAGTGCTAAAAGAAAAAGTG+AGG - chr4.2:28079545-28079564 MS.gene031524:CDS 30.0%
TCAATATATGCTATGAAGCA+CGG + chr4.2:28080095-28080114 None:intergenic 30.0%
TGCACAATAGATGAAAAGAT+TGG - chr4.2:28081304-28081323 MS.gene031524:intron 30.0%
TTCTTTAATGAATTAGGTGG+TGG - chr4.2:28080685-28080704 MS.gene031524:CDS 30.0%
! ATCTAAAATTGCTAGACTCA+TGG - chr4.2:28081520-28081539 MS.gene031524:intron 30.0%
! ATTGATTCAATCCAACGTTT+GGG - chr4.2:28079622-28079641 MS.gene031524:CDS 30.0%
! GAATAGTTGAAGCTTACTTT+TGG - chr4.2:28080751-28080770 MS.gene031524:CDS 30.0%
! GGTTTGTGACATTTTTGTTA+GGG + chr4.2:28079582-28079601 None:intergenic 30.0%
! TTTTCACTCAAGCAATTGAA+AGG - chr4.2:28080898-28080917 MS.gene031524:CDS 30.0%
!! ATTTTGCTATAAAGCCGTTT+TGG - chr4.2:28081056-28081075 MS.gene031524:CDS 30.0%
!! GAAGCTTACTTTTGGATATT+GGG - chr4.2:28080759-28080778 MS.gene031524:intron 30.0%
!! TGAAGCTTACTTTTGGATAT+TGG - chr4.2:28080758-28080777 MS.gene031524:CDS 30.0%
!!! AGATGAAGCATTGACAATTT+TGG + chr4.2:28081504-28081523 None:intergenic 30.0%
!!! ATGAAGCATTGACAATTTTG+GGG + chr4.2:28081502-28081521 None:intergenic 30.0%
!!! ATTGACAATTTTGGGGTTAT+TGG + chr4.2:28081495-28081514 None:intergenic 30.0%
!!! GATGAAGCATTGACAATTTT+GGG + chr4.2:28081503-28081522 None:intergenic 30.0%
AAAATGTCACAAACCCTTTG+AGG - chr4.2:28079587-28079606 MS.gene031524:CDS 35.0%
AAATGTCACAAACCCTTTGA+GGG - chr4.2:28079588-28079607 MS.gene031524:CDS 35.0%
AAGAAAAGGACTCAATTGAG+TGG + chr4.2:28080394-28080413 None:intergenic 35.0%
AAGAAGTTCAAGAATGAGCA+AGG - chr4.2:28080234-28080253 MS.gene031524:intron 35.0%
ACAAATTGAGAACTCGCATA+AGG + chr4.2:28081820-28081839 None:intergenic 35.0%
AGACAAGTGCAATTGAATGA+AGG + chr4.2:28079741-28079760 None:intergenic 35.0%
ATTCAATTGCACTTGTCTTC+AGG - chr4.2:28079743-28079762 MS.gene031524:CDS 35.0%
CAATGTCATGCTCAAAATGA+TGG + chr4.2:28079654-28079673 None:intergenic 35.0%
CTAATACAAGTAGACAAGGT+GGG + chr4.2:28079809-28079828 None:intergenic 35.0%
GAAACCTCTTATTAAACCGA+TGG + chr4.2:28080121-28080140 None:intergenic 35.0%
GCATTACATTTCCACTTATG+AGG - chr4.2:28080479-28080498 MS.gene031524:intron 35.0%
GCTCTTGGAATAGTAAATTC+TGG - chr4.2:28081395-28081414 MS.gene031524:intron 35.0%
GGATGATATTGTTTCCAATG+AGG - chr4.2:28081541-28081560 MS.gene031524:intron 35.0%
GTCATTCTTTGTTGATCCTT+GGG + chr4.2:28081891-28081910 None:intergenic 35.0%
TAATACAAGTAGACAAGGTG+GGG + chr4.2:28079808-28079827 None:intergenic 35.0%
TCTAATACAAGTAGACAAGG+TGG + chr4.2:28079810-28079829 None:intergenic 35.0%
TGTCATTCTTTGTTGATCCT+TGG + chr4.2:28081892-28081911 None:intergenic 35.0%
TTTGTGCTTGATTACCTCAT+TGG + chr4.2:28081558-28081577 None:intergenic 35.0%
! AATTAGTTTGAGCAAGAGAG+AGG - chr4.2:28081647-28081666 MS.gene031524:intron 35.0%
! ACAAGGATATCACATTTTGC+CGG - chr4.2:28079777-28079796 MS.gene031524:CDS 35.0%
! ATAGATGAAAAGATTGGTCC+AGG - chr4.2:28081310-28081329 MS.gene031524:intron 35.0%
! ATCAAATTAGCTTCCCTCAA+AGG + chr4.2:28079604-28079623 None:intergenic 35.0%
! ATTAGTTTGAGCAAGAGAGA+GGG - chr4.2:28081648-28081667 MS.gene031524:intron 35.0%
! CATCATTTTGAGCATGACAT+TGG - chr4.2:28079652-28079671 MS.gene031524:CDS 35.0%
! GATTGATTCAATCCAACGTT+TGG - chr4.2:28079621-28079640 MS.gene031524:CDS 35.0%
! GGGTTTGTGACATTTTTGTT+AGG + chr4.2:28079583-28079602 None:intergenic 35.0%
! TACTCTTTTTAGATGTGTCC+TGG - chr4.2:28079937-28079956 MS.gene031524:CDS 35.0%
! TCAAATTAGCTTCCCTCAAA+GGG + chr4.2:28079603-28079622 None:intergenic 35.0%
!! ATCTCCATCGGTTTAATAAG+AGG - chr4.2:28080114-28080133 MS.gene031524:CDS 35.0%
!! CTACAGAAGAGGTTTTTCAA+TGG - chr4.2:28081465-28081484 MS.gene031524:intron 35.0%
AACAACATCCTTCGTTGGAA+TGG - chr4.2:28081433-28081452 MS.gene031524:intron 40.0%
AATGACCAAGGTGATATCCT+TGG - chr4.2:28080821-28080840 MS.gene031524:intron 40.0%
ACAACATCCTTCGTTGGAAT+GGG - chr4.2:28081434-28081453 MS.gene031524:intron 40.0%
ACAAGTCACAAATGCTTGGA+AGG - chr4.2:28081748-28081767 MS.gene031524:CDS 40.0%
ACATATGAGGATTCATGGAG+AGG - chr4.2:28080317-28080336 MS.gene031524:intron 40.0%
ACTATTCTGTTGCGAGCAAA+AGG + chr4.2:28080738-28080757 None:intergenic 40.0%
AGCAAAGTTGCATCATGAGA+GGG + chr4.2:28080510-28080529 None:intergenic 40.0%
ATCACAACAACATCCTTCGT+TGG - chr4.2:28081428-28081447 MS.gene031524:intron 40.0%
ATCATCAATTACTGAGGCCA+AGG + chr4.2:28080841-28080860 None:intergenic 40.0%
CAAGACAAGTCACAAATGCT+TGG - chr4.2:28081744-28081763 MS.gene031524:CDS 40.0%
CACTCTTCAAGAAATTGCCT+AGG - chr4.2:28080445-28080464 MS.gene031524:CDS 40.0%
CATACTTTACTGAGGCTCAA+TGG - chr4.2:28081333-28081352 MS.gene031524:intron 40.0%
CTAGGCAATTTCTTGAAGAG+TGG + chr4.2:28080447-28080466 None:intergenic 40.0%
GAGTAGTGTTCGTGTTTCAT+AGG + chr4.2:28079922-28079941 None:intergenic 40.0%
GAGTGCTACATGAATGAACA+TGG - chr4.2:28081689-28081708 MS.gene031524:CDS 40.0%
GATGGTATAATCCCAAACGT+TGG + chr4.2:28079636-28079655 None:intergenic 40.0%
GATTGCTATACTAACTCCCA+AGG - chr4.2:28081872-28081891 MS.gene031524:CDS 40.0%
GGTCATAATTTCAAGGTGTG+AGG + chr4.2:28080373-28080392 None:intergenic 40.0%
TAGCAAAGTTGCATCATGAG+AGG + chr4.2:28080511-28080530 None:intergenic 40.0%
TAGGTGGGATATAAGTTGCA+TGG - chr4.2:28081011-28081030 MS.gene031524:CDS 40.0%
TCCATGAATCCTCATATGTG+TGG + chr4.2:28080316-28080335 None:intergenic 40.0%
TGAAGAAATGGAGCAAGAGA+TGG - chr4.2:28081086-28081105 MS.gene031524:CDS 40.0%
TGCTTGGAAGGATATCAATG+AGG - chr4.2:28081760-28081779 MS.gene031524:CDS 40.0%
TGGTAGAAGGATAATGACCA+AGG - chr4.2:28080809-28080828 MS.gene031524:intron 40.0%
TGTATGAAGCCACACATATG+AGG - chr4.2:28080304-28080323 MS.gene031524:intron 40.0%
TTTGAGCCTCAATACTCTGT+TGG - chr4.2:28080789-28080808 MS.gene031524:intron 40.0%
! ACCAACAATAGAGGAGTACA+TGG - chr4.2:28081373-28081392 MS.gene031524:intron 40.0%
! CAAGGATATCACATTTTGCC+GGG - chr4.2:28079778-28079797 MS.gene031524:CDS 40.0%
! CACTTGTCTTCAGGTTACTA+AGG - chr4.2:28079752-28079771 MS.gene031524:CDS 40.0%
! CTCAATACTCTGTTGGTAGA+AGG - chr4.2:28080796-28080815 MS.gene031524:intron 40.0%
! TTCAGGTTACTAAGGCAACA+AGG - chr4.2:28079760-28079779 MS.gene031524:CDS 40.0%
!! AACACTTGTTGGTGATGTTG+AGG - chr4.2:28080269-28080288 MS.gene031524:intron 40.0%
!! ACACTTGTTGGTGATGTTGA+GGG - chr4.2:28080270-28080289 MS.gene031524:intron 40.0%
!! CTTCTACCAACAGAGTATTG+AGG + chr4.2:28080798-28080817 None:intergenic 40.0%
AATGCATCCCATTCCAACGA+AGG + chr4.2:28081444-28081463 None:intergenic 45.0%
AATGGAGCAAGAGATGGTCA+AGG - chr4.2:28081092-28081111 MS.gene031524:CDS 45.0%
AGAGGGATCTTCCTCATAAG+TGG + chr4.2:28080493-28080512 None:intergenic 45.0%
ATGAAGCACGGATACAGACA+TGG + chr4.2:28080083-28080102 None:intergenic 45.0%
CAAGAAATTGCCTAGGCTAC+TGG - chr4.2:28080452-28080471 MS.gene031524:intron 45.0%
CATGTAGCACTCAATAGCAG+AGG + chr4.2:28081681-28081700 None:intergenic 45.0%
GCCACACATATGAGGATTCA+TGG - chr4.2:28080312-28080331 MS.gene031524:intron 45.0%
GCCATGTACTCCTCTATTGT+TGG + chr4.2:28081377-28081396 None:intergenic 45.0%
TTGAGAACTCGCATAAGGAG+AGG + chr4.2:28081815-28081834 None:intergenic 45.0%
! AGAGGCTTTGGCACTTGAAT+TGG + chr4.2:28081797-28081816 None:intergenic 45.0%
! ATAGAGGAGTACATGGCTCT+TGG - chr4.2:28081380-28081399 MS.gene031524:intron 45.0%
! GTGTCTGAAACGTCTCGAAT+AGG + chr4.2:28080637-28080656 None:intergenic 45.0%
!! AAAATATAAATTTAAAAGAT+AGG + chr4.2:28081598-28081617 None:intergenic 5.0%
!! ATAAAAAAAATAAAAAAACA+AGG + chr4.2:28081181-28081200 None:intergenic 5.0%
AATTGCCTAGGCTACTGGCA+AGG - chr4.2:28080457-28080476 MS.gene031524:intron 50.0%
ACTCGCATAAGGAGAGGCTT+TGG + chr4.2:28081809-28081828 None:intergenic 50.0%
ATCATGTGTCAGAGTCGCGT+CGG + chr4.2:28079988-28080007 None:intergenic 50.0%
GAGCAAGAGATGGTCAAGGA+AGG - chr4.2:28081096-28081115 MS.gene031524:CDS 50.0%
GAGCCTCAGTAAAGTATGCC+TGG + chr4.2:28081331-28081350 None:intergenic 50.0%
GGGATGCATTGCTACAGAAG+AGG - chr4.2:28081454-28081473 MS.gene031524:intron 50.0%
GGTCCAGGCATACTTTACTG+AGG - chr4.2:28081325-28081344 MS.gene031524:intron 50.0%
TAATGCCTTGCCAGTAGCCT+AGG + chr4.2:28080465-28080484 None:intergenic 50.0%
TAGACAAGGTGGGGTTTACC+CGG + chr4.2:28079799-28079818 None:intergenic 50.0%
TGAGGCCAAGGATATCACCT+TGG + chr4.2:28080829-28080848 None:intergenic 50.0%
TTTGAGCAAGAGAGAGGGCA+TGG - chr4.2:28081653-28081672 MS.gene031524:intron 50.0%
AACGACACGTGTTCGACACC+AGG + chr4.2:28079958-28079977 None:intergenic 55.0%
! TGTCAGAGTCGCGTCGGTGT+TGG + chr4.2:28079982-28080001 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 28079532 28081946 28079532 ID=MS.gene031524
chr4.2 mRNA 28079532 28081946 28079532 ID=MS.gene031524.t1;Parent=MS.gene031524
chr4.2 exon 28081679 28081946 28081679 ID=MS.gene031524.t1.exon1;Parent=MS.gene031524.t1
chr4.2 CDS 28081679 28081946 28081679 ID=cds.MS.gene031524.t1;Parent=MS.gene031524.t1
chr4.2 exon 28080880 28081252 28080880 ID=MS.gene031524.t1.exon2;Parent=MS.gene031524.t1
chr4.2 CDS 28080880 28081252 28080880 ID=cds.MS.gene031524.t1;Parent=MS.gene031524.t1
chr4.2 exon 28080559 28080777 28080559 ID=MS.gene031524.t1.exon3;Parent=MS.gene031524.t1
chr4.2 CDS 28080559 28080777 28080559 ID=cds.MS.gene031524.t1;Parent=MS.gene031524.t1
chr4.2 exon 28080326 28080464 28080326 ID=MS.gene031524.t1.exon4;Parent=MS.gene031524.t1
chr4.2 CDS 28080326 28080464 28080326 ID=cds.MS.gene031524.t1;Parent=MS.gene031524.t1
chr4.2 exon 28079916 28080164 28079916 ID=MS.gene031524.t1.exon5;Parent=MS.gene031524.t1
chr4.2 CDS 28079916 28080164 28079916 ID=cds.MS.gene031524.t1;Parent=MS.gene031524.t1
chr4.2 exon 28079532 28079819 28079532 ID=MS.gene031524.t1.exon6;Parent=MS.gene031524.t1
chr4.2 CDS 28079532 28079819 28079532 ID=cds.MS.gene031524.t1;Parent=MS.gene031524.t1
Gene Sequence

>MS.gene031524

ATGAAGCAGATTATAGTGCTAAAAGAAAAAGTGAGGCAGATGCTAGTCCCTAACAAAAATGTCACAAACCCTTTGAGGGAAGCTAATTTGATTGATTCAATCCAACGTTTGGGATTATACCATCATTTTGAGCATGACATTGGAGAATTATTGCGACATATTCACAATAATCATGTTGAAAATGGAACAATAACTCTTAACGAAGACCTTCATTCAATTGCACTTGTCTTCAGGTTACTAAGGCAACAAGGATATCACATTTTGCCGGGTAAACCCCACCTTGTCTACTTGTATTAGATTTTAGTCTTATTTTACGTAAATGTATACAACACATTAAACATCTAAATTCAAAGTGCTTAAAGCGTGCTCAGATGAGATGACATATATCCTATGAAACACGAACACTACTCTTTTTAGATGTGTCCTGGTGTCGAACACGTGTCGTTTCCAACACCGACGCGACTCTGACACATGATTACACCGAATTATGTGATTTTTTAAAATTATTAGTGGTGTCAGTGTGTTAGTGTCTGTATCATATTCGGTGTCCATGTCTGTATCCGTGCTTCATAGCATATATTGATCTCCATCGGTTTAATAAGAGGTTTCAAGTCACGTGAACTTAACAAAATTAAAATGATTAAATGGTTTGAAGAACATAAAACTTAACCATATATTATCATGTGGTTCTCAGATGCTTTTAAGAAGTTCAAGAATGAGCAAGGAAACTTCAAAGAAACACTTGTTGGTGATGTTGAGGGAATGCTAAGTTTGTATGAAGCCACACATATGAGGATTCATGGAGAGGATATATTAGATGAAGCACTTTCTTTCACTTCCTCACACCTTGAAATTATGACCACTCAATTGAGTCCTTTTCTTGCTACAAAAATTAATCATAGCTTAAAGCGGCCACTCTTCAAGAAATTGCCTAGGCTACTGGCAAGGCATTACATTTCCACTTATGAGGAAGATCCCTCTCATGATGCAACTTTGCTATTACTTGCCAAATTAGATTTCAATTTGCTTCAAAAACAACATCAGAAAGAAGTTGGCGATATTTCAAAGTAAGTTTTTTTTTTTTTATTCTTTTAAAGTTAATCCTATTCGAGACGTTTCAGACACTAAATATGACTATCAAACTAAGTTTGTTTTCTTTAATGAATTAGGTGGTGGAAAGATTTAGATTTTGCAACAAAACTACCTTTTGCTCGCAACAGAATAGTTGAAGCTTACTTTTGGATATTGGGAGTGTATTTTGAGCCTCAATACTCTGTTGGTAGAAGGATAATGACCAAGGTGATATCCTTGGCCTCAGTAATTGATGATATATATGATGTTTATGGTACAATAGAAGAGCTACAACTTTTCACTCAAGCAATTGAAAGGTTTGAATCTCTACCAAATTTTCACATCTCAATATTGAACTATTTTTTTATGGAAATTTATTAACCTCTTAATTAATTTGAATTAAAATTCTAGGTGGGATATAAGTTGCATGGATTTTCTACCACAATACATGAAATTTTGCTATAAAGCCGTTTTGGATGTTTATGAAGAAATGGAGCAAGAGATGGTCAAGGAAGGCAGAGCATTTTGTGTAACATATGCCAAAAATGAAGTAAAAATTCTTACTCTTCATTTTTCCTTGTTTTTTTATTTTTTTTATTTTAAAAGAGATTAAAATGAGAGAATATTTTTTCTTGCAAGATTAAAATGAGAAAATGTGCTTCATGTCTAACTCATATTCTTGTAAATTGTTTTGGTTCATGTGCACAATAGATGAAAAGATTGGTCCAGGCATACTTTACTGAGGCTCAATGGTTCAGTAGAAAATATATACCAACAATAGAGGAGTACATGGCTCTTGGAATAGTAAATTCTGGTTACTATCTAATCACAACAACATCCTTCGTTGGAATGGGATGCATTGCTACAGAAGAGGTTTTTCAATGGTTAACCAATAACCCCAAAATTGTCAATGCTTCATCTAAAATTGCTAGACTCATGGATGATATTGTTTCCAATGAGGTAATCAAGCACAAAAATTAATTTATCAATTTCCTATCTTTTAAATTTATATTTTAAATAAATATGTTTATTTTTTTTTATATTAATTAGTTTGAGCAAGAGAGAGGGCATGGTGCCTCTGCTATTGAGTGCTACATGAATGAACATGGTGTGTCTAGAGAAGATGCAGTTAATGAACTTTCAAGACAAGTCACAAATGCTTGGAAGGATATCAATGAGGAACTTCTTGATCCAATTCAAGTGCCAAAGCCTCTCCTTATGCGAGTTCTCAATTTGTCACGTGTCATTGATGTGCTTTATAAAGATGGAGATTGCTATACTAACTCCCAAGGATCAACAAAGAATGACATAATATCTATCTTGTTGAATCCATGTCCAATATGA
Protein sequence

>MS.gene031524.t1

MKQIIVLKEKVRQMLVPNKNVTNPLREANLIDSIQRLGLYHHFEHDIGELLRHIHNNHVENGTITLNEDLHSIALVFRLLRQQGYHILPDAFKKFKNEQGNFKETLVGDVEGMLSLYEATHMRIHGEDILDEALSFTSSHLEIMTTQLSPFLATKINHSLKRPLFKKLPRLLARHYISTYEEDPSHDATLLLLAKLDFNLLQKQHQKEVGDISKWWKDLDFATKLPFARNRIVEAYFWILGVYFEPQYSVGRRIMTKVISLASVIDDIYDVYGTIEELQLFTQAIERWDISCMDFLPQYMKFCYKAVLDVYEEMEQEMVKEGRAFCVTYAKNEMKRLVQAYFTEAQWFSRKYIPTIEEYMALGIVNSGYYLITTTSFVGMGCIATEEVFQWLTNNPKIVNASSKIARLMDDIVSNEQERGHGASAIECYMNEHGVSREDAVNELSRQVTNAWKDINEELLDPIQVPKPLLMRVLNLSRVIDVLYKDGDCYTNSQGSTKNDIISILLNPCPI