Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08826.t1 | XP_003629616.1 | 95.8 | 119 | 4 | 1 | 1 | 118 | 1 | 119 | 9.50E-55 | 222.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08826.t1 | O23262 | 50.0 | 94 | 39 | 3 | 31 | 118 | 26 | 117 | 6.1e-18 | 91.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08826.t1 | B7FMN1 | 95.8 | 119 | 4 | 1 | 1 | 118 | 1 | 119 | 6.8e-55 | 222.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049227 | MS.gene08826 | 0.819512 | 1.12E-52 | -1.69E-46 |
| MS.gene051234 | MS.gene08826 | 0.849139 | 3.92E-60 | -1.69E-46 |
| MS.gene053143 | MS.gene08826 | 0.809322 | 2.00E-50 | -1.69E-46 |
| MS.gene053536 | MS.gene08826 | 0.808602 | 2.86E-50 | -1.69E-46 |
| MS.gene053538 | MS.gene08826 | 0.806391 | 8.41E-50 | -1.69E-46 |
| MS.gene054617 | MS.gene08826 | 0.800126 | 1.67E-48 | -1.69E-46 |
| MS.gene055220 | MS.gene08826 | 0.814372 | 1.59E-51 | -1.69E-46 |
| MS.gene055221 | MS.gene08826 | 0.822902 | 1.85E-53 | -1.69E-46 |
| MS.gene056266 | MS.gene08826 | -0.801757 | 7.74E-49 | -1.69E-46 |
| MS.gene056998 | MS.gene08826 | 0.808654 | 2.78E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene08826 | 0.810589 | 1.07E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene08826 | 0.808951 | 2.41E-50 | -1.69E-46 |
| MS.gene059778 | MS.gene08826 | 0.829937 | 3.89E-55 | -1.69E-46 |
| MS.gene061100 | MS.gene08826 | 0.816391 | 5.66E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08826 | MS.gene035341 | PPI |
| MS.gene08826 | MS.gene041566 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08826.t1 | MTR_8g083150 | 95.798 | 119 | 4 | 1 | 1 | 118 | 1 | 119 | 1.30e-80 | 232 |
| MS.gene08826.t1 | MTR_8g083150 | 95.690 | 116 | 4 | 1 | 1 | 115 | 1 | 116 | 1.24e-78 | 227 |
| MS.gene08826.t1 | MTR_4g119860 | 37.500 | 120 | 53 | 5 | 14 | 115 | 10 | 125 | 4.53e-14 | 63.9 |
| MS.gene08826.t1 | MTR_7g108600 | 35.294 | 119 | 62 | 4 | 10 | 117 | 4 | 118 | 1.85e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08826.t1 | AT4G14010 | 46.087 | 115 | 47 | 5 | 10 | 118 | 12 | 117 | 1.01e-25 | 93.6 |
| MS.gene08826.t1 | AT1G02900 | 38.400 | 125 | 63 | 4 | 1 | 116 | 1 | 120 | 3.87e-15 | 66.6 |
| MS.gene08826.t1 | AT4G15800 | 46.341 | 82 | 37 | 3 | 37 | 115 | 38 | 115 | 3.28e-13 | 61.6 |
Find 20 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGAACATCCTCTATTATA+AGG | 0.298490 | 4.2:+13353406 | None:intergenic |
| GTTCTAAGTATTATCGTTGT+AGG | 0.352031 | 4.2:-13353390 | MS.gene08826:CDS |
| TCAAGAAGATGAACAATTGA+TGG | 0.375070 | 4.2:-13353572 | MS.gene08826:CDS |
| CCTCTATTATAAGGATTTGA+AGG | 0.425611 | 4.2:+13353415 | None:intergenic |
| GCATGAAGTAGAAGAAGGTC+AGG | 0.441178 | 4.2:+13353686 | None:intergenic |
| CCTGCACCACCATTACAAAC+TGG | 0.451169 | 4.2:+13353478 | None:intergenic |
| TTCTTGATTGCATTCAGCTA+TGG | 0.463317 | 4.2:+13353588 | None:intergenic |
| GAAGCTTATAGTAAATCTGC+AGG | 0.482571 | 4.2:-13353451 | MS.gene08826:CDS |
| CCTTCAAATCCTTATAATAG+AGG | 0.483889 | 4.2:-13353415 | MS.gene08826:CDS |
| CCAGTTTGTAATGGTGGTGC+AGG | 0.525366 | 4.2:-13353478 | MS.gene08826:CDS |
| GATAAACCAGTTTGTAATGG+TGG | 0.525715 | 4.2:-13353484 | MS.gene08826:CDS |
| AGAGATAAACCAGTTTGTAA+TGG | 0.535103 | 4.2:-13353487 | MS.gene08826:CDS |
| TTATAAGGATTTGAAGGTGG+AGG | 0.542122 | 4.2:+13353421 | None:intergenic |
| GAAGAGCATGAAGTAGAAGA+AGG | 0.591507 | 4.2:+13353681 | None:intergenic |
| CAAAGGAGATACATTTCTGA+AGG | 0.595659 | 4.2:-13353520 | MS.gene08826:CDS |
| CTATTATAAGGATTTGAAGG+TGG | 0.614457 | 4.2:+13353418 | None:intergenic |
| CATCATGCAAGCATATGCAA+TGG | 0.634173 | 4.2:-13353613 | MS.gene08826:CDS |
| TGGAGTCAGAAATAAGCAGA+AGG | 0.637756 | 4.2:-13353552 | MS.gene08826:CDS |
| GTTTGTAATGGTGGTGCAGG+TGG | 0.645633 | 4.2:-13353475 | MS.gene08826:CDS |
| AAGCTTATAGTAAATCTGCA+GGG | 0.695265 | 4.2:-13353450 | MS.gene08826:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTAGAACATCCTCTATTATA+AGG | + | chr4.2:13353687-13353706 | None:intergenic | 25.0% |
| AAGCTTATAGTAAATCTGCA+GGG | - | chr4.2:13353640-13353659 | MS.gene08826:CDS | 30.0% | |
| AGAGATAAACCAGTTTGTAA+TGG | - | chr4.2:13353603-13353622 | MS.gene08826:CDS | 30.0% | |
| CCTCTATTATAAGGATTTGA+AGG | + | chr4.2:13353678-13353697 | None:intergenic | 30.0% | |
| CCTTCAAATCCTTATAATAG+AGG | - | chr4.2:13353675-13353694 | MS.gene08826:CDS | 30.0% | |
| CTATTATAAGGATTTGAAGG+TGG | + | chr4.2:13353675-13353694 | None:intergenic | 30.0% | |
| TCAAGAAGATGAACAATTGA+TGG | - | chr4.2:13353518-13353537 | MS.gene08826:CDS | 30.0% | |
| ! | AGAAATAAGCAGAAGGTTTT+TGG | - | chr4.2:13353545-13353564 | MS.gene08826:CDS | 30.0% |
| ! | GTTCTAAGTATTATCGTTGT+AGG | - | chr4.2:13353700-13353719 | MS.gene08826:CDS | 30.0% |
| CAAAGGAGATACATTTCTGA+AGG | - | chr4.2:13353570-13353589 | MS.gene08826:CDS | 35.0% | |
| GAAGCTTATAGTAAATCTGC+AGG | - | chr4.2:13353639-13353658 | MS.gene08826:CDS | 35.0% | |
| GATAAACCAGTTTGTAATGG+TGG | - | chr4.2:13353606-13353625 | MS.gene08826:CDS | 35.0% | |
| TTATAAGGATTTGAAGGTGG+AGG | + | chr4.2:13353672-13353691 | None:intergenic | 35.0% | |
| TTCTTGATTGCATTCAGCTA+TGG | + | chr4.2:13353505-13353524 | None:intergenic | 35.0% | |
| CATCATGCAAGCATATGCAA+TGG | - | chr4.2:13353477-13353496 | MS.gene08826:CDS | 40.0% | |
| TGGAGTCAGAAATAAGCAGA+AGG | - | chr4.2:13353538-13353557 | MS.gene08826:CDS | 40.0% | |
| ! | GAAGAGCATGAAGTAGAAGA+AGG | + | chr4.2:13353412-13353431 | None:intergenic | 40.0% |
| GCATGAAGTAGAAGAAGGTC+AGG | + | chr4.2:13353407-13353426 | None:intergenic | 45.0% | |
| !!! | GCAGAAGGTTTTTGGAGCAA+AGG | - | chr4.2:13353553-13353572 | MS.gene08826:CDS | 45.0% |
| CCTGCACCACCATTACAAAC+TGG | + | chr4.2:13353615-13353634 | None:intergenic | 50.0% | |
| ! | CCAGTTTGTAATGGTGGTGC+AGG | - | chr4.2:13353612-13353631 | MS.gene08826:CDS | 50.0% |
| ! | GTTTGTAATGGTGGTGCAGG+TGG | - | chr4.2:13353615-13353634 | MS.gene08826:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 13353378 | 13353734 | 13353378 | ID=MS.gene08826 |
| chr4.2 | mRNA | 13353378 | 13353734 | 13353378 | ID=MS.gene08826.t1;Parent=MS.gene08826 |
| chr4.2 | exon | 13353378 | 13353734 | 13353378 | ID=MS.gene08826.t1.exon1;Parent=MS.gene08826.t1 |
| chr4.2 | CDS | 13353378 | 13353734 | 13353378 | ID=cds.MS.gene08826.t1;Parent=MS.gene08826.t1 |
| Gene Sequence |
| Protein sequence |
