Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08980.t1 | XP_003629616.1 | 95.8 | 119 | 4 | 1 | 1 | 118 | 1 | 119 | 9.50E-55 | 222.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08980.t1 | O23262 | 50.0 | 94 | 39 | 3 | 31 | 118 | 26 | 117 | 6.1e-18 | 91.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08980.t1 | B7FMN1 | 95.8 | 119 | 4 | 1 | 1 | 118 | 1 | 119 | 6.8e-55 | 222.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049227 | MS.gene08980 | 0.822027 | 2.95E-53 | -1.69E-46 |
| MS.gene051234 | MS.gene08980 | 0.848579 | 5.60E-60 | -1.69E-46 |
| MS.gene053143 | MS.gene08980 | 0.810144 | 1.33E-50 | -1.69E-46 |
| MS.gene053536 | MS.gene08980 | 0.811844 | 5.71E-51 | -1.69E-46 |
| MS.gene053538 | MS.gene08980 | 0.809316 | 2.01E-50 | -1.69E-46 |
| MS.gene054617 | MS.gene08980 | 0.803938 | 2.75E-49 | -1.69E-46 |
| MS.gene055220 | MS.gene08980 | 0.813656 | 2.29E-51 | -1.69E-46 |
| MS.gene055221 | MS.gene08980 | 0.822227 | 2.65E-53 | -1.69E-46 |
| MS.gene056998 | MS.gene08980 | 0.808112 | 3.63E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene08980 | 0.808123 | 3.62E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene08980 | 0.808934 | 2.43E-50 | -1.69E-46 |
| MS.gene059778 | MS.gene08980 | 0.826065 | 3.33E-54 | -1.69E-46 |
| MS.gene061100 | MS.gene08980 | 0.817917 | 2.57E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08980 | MS.gene035341 | PPI |
| MS.gene041566 | MS.gene08980 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08980.t1 | MTR_8g083150 | 95.798 | 119 | 4 | 1 | 1 | 118 | 1 | 119 | 1.30e-80 | 232 |
| MS.gene08980.t1 | MTR_8g083150 | 95.690 | 116 | 4 | 1 | 1 | 115 | 1 | 116 | 1.24e-78 | 227 |
| MS.gene08980.t1 | MTR_4g119860 | 37.500 | 120 | 53 | 5 | 14 | 115 | 10 | 125 | 4.53e-14 | 63.9 |
| MS.gene08980.t1 | MTR_7g108600 | 35.294 | 119 | 62 | 4 | 10 | 117 | 4 | 118 | 1.85e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08980.t1 | AT4G14010 | 46.087 | 115 | 47 | 5 | 10 | 118 | 12 | 117 | 1.01e-25 | 93.6 |
| MS.gene08980.t1 | AT1G02900 | 38.400 | 125 | 63 | 4 | 1 | 116 | 1 | 120 | 3.87e-15 | 66.6 |
| MS.gene08980.t1 | AT4G15800 | 46.341 | 82 | 37 | 3 | 37 | 115 | 38 | 115 | 3.28e-13 | 61.6 |
Find 20 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGAACATCCTCTATTATA+AGG | 0.298490 | 4.4:+14926918 | None:intergenic |
| GTTCTAAGTATTATCGTTGT+AGG | 0.352031 | 4.4:-14926902 | MS.gene08980:CDS |
| TCAAGAAGATGAACAATTGA+TGG | 0.375070 | 4.4:-14927084 | MS.gene08980:CDS |
| CCTCTATTATAAGGATTTGA+AGG | 0.425611 | 4.4:+14926927 | None:intergenic |
| GCATGAAGTAGAAGAAGGTC+AGG | 0.441178 | 4.4:+14927198 | None:intergenic |
| CCTGCACCACCATTACAAAC+TGG | 0.451169 | 4.4:+14926990 | None:intergenic |
| TTCTTGATTGCATTCAGCTA+TGG | 0.463317 | 4.4:+14927100 | None:intergenic |
| GAAGCTTATAGTAAATCTGC+AGG | 0.482571 | 4.4:-14926963 | MS.gene08980:CDS |
| CCTTCAAATCCTTATAATAG+AGG | 0.483889 | 4.4:-14926927 | MS.gene08980:CDS |
| CCAGTTTGTAATGGTGGTGC+AGG | 0.525366 | 4.4:-14926990 | MS.gene08980:CDS |
| GATAAACCAGTTTGTAATGG+TGG | 0.525715 | 4.4:-14926996 | MS.gene08980:CDS |
| AGAGATAAACCAGTTTGTAA+TGG | 0.535103 | 4.4:-14926999 | MS.gene08980:CDS |
| TTATAAGGATTTGAAGGTGG+AGG | 0.542122 | 4.4:+14926933 | None:intergenic |
| GAAGAGCATGAAGTAGAAGA+AGG | 0.591507 | 4.4:+14927193 | None:intergenic |
| CAAAGGAGATACATTTCTGA+AGG | 0.595659 | 4.4:-14927032 | MS.gene08980:CDS |
| CTATTATAAGGATTTGAAGG+TGG | 0.614457 | 4.4:+14926930 | None:intergenic |
| CATCATGCAAGCATATGCAA+TGG | 0.634173 | 4.4:-14927125 | MS.gene08980:CDS |
| TGGAGTCAGAAATAAGCAGA+AGG | 0.637756 | 4.4:-14927064 | MS.gene08980:CDS |
| GTTTGTAATGGTGGTGCAGG+TGG | 0.645633 | 4.4:-14926987 | MS.gene08980:CDS |
| AAGCTTATAGTAAATCTGCA+GGG | 0.695265 | 4.4:-14926962 | MS.gene08980:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTAGAACATCCTCTATTATA+AGG | + | chr4.4:14927199-14927218 | None:intergenic | 25.0% |
| AAGCTTATAGTAAATCTGCA+GGG | - | chr4.4:14927152-14927171 | MS.gene08980:CDS | 30.0% | |
| AGAGATAAACCAGTTTGTAA+TGG | - | chr4.4:14927115-14927134 | MS.gene08980:CDS | 30.0% | |
| CCTCTATTATAAGGATTTGA+AGG | + | chr4.4:14927190-14927209 | None:intergenic | 30.0% | |
| CCTTCAAATCCTTATAATAG+AGG | - | chr4.4:14927187-14927206 | MS.gene08980:CDS | 30.0% | |
| CTATTATAAGGATTTGAAGG+TGG | + | chr4.4:14927187-14927206 | None:intergenic | 30.0% | |
| TCAAGAAGATGAACAATTGA+TGG | - | chr4.4:14927030-14927049 | MS.gene08980:CDS | 30.0% | |
| ! | AGAAATAAGCAGAAGGTTTT+TGG | - | chr4.4:14927057-14927076 | MS.gene08980:CDS | 30.0% |
| ! | GTTCTAAGTATTATCGTTGT+AGG | - | chr4.4:14927212-14927231 | MS.gene08980:CDS | 30.0% |
| CAAAGGAGATACATTTCTGA+AGG | - | chr4.4:14927082-14927101 | MS.gene08980:CDS | 35.0% | |
| GAAGCTTATAGTAAATCTGC+AGG | - | chr4.4:14927151-14927170 | MS.gene08980:CDS | 35.0% | |
| GATAAACCAGTTTGTAATGG+TGG | - | chr4.4:14927118-14927137 | MS.gene08980:CDS | 35.0% | |
| TTATAAGGATTTGAAGGTGG+AGG | + | chr4.4:14927184-14927203 | None:intergenic | 35.0% | |
| TTCTTGATTGCATTCAGCTA+TGG | + | chr4.4:14927017-14927036 | None:intergenic | 35.0% | |
| CATCATGCAAGCATATGCAA+TGG | - | chr4.4:14926989-14927008 | MS.gene08980:CDS | 40.0% | |
| TGGAGTCAGAAATAAGCAGA+AGG | - | chr4.4:14927050-14927069 | MS.gene08980:CDS | 40.0% | |
| ! | GAAGAGCATGAAGTAGAAGA+AGG | + | chr4.4:14926924-14926943 | None:intergenic | 40.0% |
| GCATGAAGTAGAAGAAGGTC+AGG | + | chr4.4:14926919-14926938 | None:intergenic | 45.0% | |
| !!! | GCAGAAGGTTTTTGGAGCAA+AGG | - | chr4.4:14927065-14927084 | MS.gene08980:CDS | 45.0% |
| CCTGCACCACCATTACAAAC+TGG | + | chr4.4:14927127-14927146 | None:intergenic | 50.0% | |
| ! | CCAGTTTGTAATGGTGGTGC+AGG | - | chr4.4:14927124-14927143 | MS.gene08980:CDS | 50.0% |
| ! | GTTTGTAATGGTGGTGCAGG+TGG | - | chr4.4:14927127-14927146 | MS.gene08980:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 14926890 | 14927246 | 14926890 | ID=MS.gene08980 |
| chr4.4 | mRNA | 14926890 | 14927246 | 14926890 | ID=MS.gene08980.t1;Parent=MS.gene08980 |
| chr4.4 | exon | 14926890 | 14927246 | 14926890 | ID=MS.gene08980.t1.exon1;Parent=MS.gene08980.t1 |
| chr4.4 | CDS | 14926890 | 14927246 | 14926890 | ID=cds.MS.gene08980.t1;Parent=MS.gene08980.t1 |
| Gene Sequence |
| Protein sequence |
