Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09266.t1 | XP_003629616.1 | 95.8 | 119 | 4 | 1 | 1 | 118 | 1 | 119 | 9.50E-55 | 222.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09266.t1 | O23262 | 50.0 | 94 | 39 | 3 | 31 | 118 | 26 | 117 | 6.1e-18 | 91.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09266.t1 | B7FMN1 | 95.8 | 119 | 4 | 1 | 1 | 118 | 1 | 119 | 6.8e-55 | 222.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049227 | MS.gene09266 | 0.81396 | 1.96E-51 | -1.69E-46 |
| MS.gene051234 | MS.gene09266 | 0.844196 | 8.78E-59 | -1.69E-46 |
| MS.gene053143 | MS.gene09266 | 0.804947 | 1.69E-49 | -1.69E-46 |
| MS.gene053536 | MS.gene09266 | 0.802439 | 5.61E-49 | -1.69E-46 |
| MS.gene053538 | MS.gene09266 | 0.803386 | 3.57E-49 | -1.69E-46 |
| MS.gene054617 | MS.gene09266 | 0.800382 | 1.48E-48 | -1.69E-46 |
| MS.gene055220 | MS.gene09266 | 0.810563 | 1.08E-50 | -1.69E-46 |
| MS.gene055221 | MS.gene09266 | 0.819631 | 1.05E-52 | -1.69E-46 |
| MS.gene056266 | MS.gene09266 | -0.810309 | 1.23E-50 | -1.69E-46 |
| MS.gene056998 | MS.gene09266 | 0.811868 | 5.64E-51 | -1.69E-46 |
| MS.gene058210 | MS.gene09266 | 0.800053 | 1.73E-48 | -1.69E-46 |
| MS.gene059778 | MS.gene09266 | 0.819294 | 1.25E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene09266 | 0.806344 | 8.61E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene041566 | MS.gene09266 | PPI |
| MS.gene09266 | MS.gene035341 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09266.t1 | MTR_8g083150 | 95.798 | 119 | 4 | 1 | 1 | 118 | 1 | 119 | 1.30e-80 | 232 |
| MS.gene09266.t1 | MTR_8g083150 | 95.690 | 116 | 4 | 1 | 1 | 115 | 1 | 116 | 1.24e-78 | 227 |
| MS.gene09266.t1 | MTR_4g119860 | 37.500 | 120 | 53 | 5 | 14 | 115 | 10 | 125 | 4.53e-14 | 63.9 |
| MS.gene09266.t1 | MTR_7g108600 | 35.294 | 119 | 62 | 4 | 10 | 117 | 4 | 118 | 1.85e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09266.t1 | AT4G14010 | 46.087 | 115 | 47 | 5 | 10 | 118 | 12 | 117 | 1.01e-25 | 93.6 |
| MS.gene09266.t1 | AT1G02900 | 38.400 | 125 | 63 | 4 | 1 | 116 | 1 | 120 | 3.87e-15 | 66.6 |
| MS.gene09266.t1 | AT4G15800 | 46.341 | 82 | 37 | 3 | 37 | 115 | 38 | 115 | 3.28e-13 | 61.6 |
Find 20 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGAACATCCTCTATTATA+AGG | 0.298490 | 4.1:+14559040 | None:intergenic |
| GTTCTAAGTATTATCGTTGT+AGG | 0.352031 | 4.1:-14559024 | MS.gene09266:CDS |
| TCAAGAAGATGAACAATTGA+TGG | 0.375070 | 4.1:-14559206 | MS.gene09266:CDS |
| CCTCTATTATAAGGATTTGA+AGG | 0.425611 | 4.1:+14559049 | None:intergenic |
| GCATGAAGTAGAAGAAGGTC+AGG | 0.441178 | 4.1:+14559320 | None:intergenic |
| CCTGCACCACCATTACAAAC+TGG | 0.451169 | 4.1:+14559112 | None:intergenic |
| TTCTTGATTGCATTCAGCTA+TGG | 0.463317 | 4.1:+14559222 | None:intergenic |
| GAAGCTTATAGTAAATCTGC+AGG | 0.482571 | 4.1:-14559085 | MS.gene09266:CDS |
| CCTTCAAATCCTTATAATAG+AGG | 0.483889 | 4.1:-14559049 | MS.gene09266:CDS |
| CCAGTTTGTAATGGTGGTGC+AGG | 0.525366 | 4.1:-14559112 | MS.gene09266:CDS |
| GATAAACCAGTTTGTAATGG+TGG | 0.525715 | 4.1:-14559118 | MS.gene09266:CDS |
| AGAGATAAACCAGTTTGTAA+TGG | 0.535103 | 4.1:-14559121 | MS.gene09266:CDS |
| TTATAAGGATTTGAAGGTGG+AGG | 0.542122 | 4.1:+14559055 | None:intergenic |
| GAAGAGCATGAAGTAGAAGA+AGG | 0.591507 | 4.1:+14559315 | None:intergenic |
| CAAAGGAGATACATTTCTGA+AGG | 0.595659 | 4.1:-14559154 | MS.gene09266:CDS |
| CTATTATAAGGATTTGAAGG+TGG | 0.614457 | 4.1:+14559052 | None:intergenic |
| CATCATGCAAGCATATGCAA+TGG | 0.634173 | 4.1:-14559247 | MS.gene09266:CDS |
| TGGAGTCAGAAATAAGCAGA+AGG | 0.637756 | 4.1:-14559186 | MS.gene09266:CDS |
| GTTTGTAATGGTGGTGCAGG+TGG | 0.645633 | 4.1:-14559109 | MS.gene09266:CDS |
| AAGCTTATAGTAAATCTGCA+GGG | 0.695265 | 4.1:-14559084 | MS.gene09266:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTAGAACATCCTCTATTATA+AGG | + | chr4.1:14559321-14559340 | None:intergenic | 25.0% |
| AAGCTTATAGTAAATCTGCA+GGG | - | chr4.1:14559274-14559293 | MS.gene09266:CDS | 30.0% | |
| AGAGATAAACCAGTTTGTAA+TGG | - | chr4.1:14559237-14559256 | MS.gene09266:CDS | 30.0% | |
| CCTCTATTATAAGGATTTGA+AGG | + | chr4.1:14559312-14559331 | None:intergenic | 30.0% | |
| CCTTCAAATCCTTATAATAG+AGG | - | chr4.1:14559309-14559328 | MS.gene09266:CDS | 30.0% | |
| CTATTATAAGGATTTGAAGG+TGG | + | chr4.1:14559309-14559328 | None:intergenic | 30.0% | |
| TCAAGAAGATGAACAATTGA+TGG | - | chr4.1:14559152-14559171 | MS.gene09266:CDS | 30.0% | |
| ! | AGAAATAAGCAGAAGGTTTT+TGG | - | chr4.1:14559179-14559198 | MS.gene09266:CDS | 30.0% |
| ! | GTTCTAAGTATTATCGTTGT+AGG | - | chr4.1:14559334-14559353 | MS.gene09266:CDS | 30.0% |
| CAAAGGAGATACATTTCTGA+AGG | - | chr4.1:14559204-14559223 | MS.gene09266:CDS | 35.0% | |
| GAAGCTTATAGTAAATCTGC+AGG | - | chr4.1:14559273-14559292 | MS.gene09266:CDS | 35.0% | |
| GATAAACCAGTTTGTAATGG+TGG | - | chr4.1:14559240-14559259 | MS.gene09266:CDS | 35.0% | |
| TTATAAGGATTTGAAGGTGG+AGG | + | chr4.1:14559306-14559325 | None:intergenic | 35.0% | |
| TTCTTGATTGCATTCAGCTA+TGG | + | chr4.1:14559139-14559158 | None:intergenic | 35.0% | |
| CATCATGCAAGCATATGCAA+TGG | - | chr4.1:14559111-14559130 | MS.gene09266:CDS | 40.0% | |
| TGGAGTCAGAAATAAGCAGA+AGG | - | chr4.1:14559172-14559191 | MS.gene09266:CDS | 40.0% | |
| ! | GAAGAGCATGAAGTAGAAGA+AGG | + | chr4.1:14559046-14559065 | None:intergenic | 40.0% |
| GCATGAAGTAGAAGAAGGTC+AGG | + | chr4.1:14559041-14559060 | None:intergenic | 45.0% | |
| !!! | GCAGAAGGTTTTTGGAGCAA+AGG | - | chr4.1:14559187-14559206 | MS.gene09266:CDS | 45.0% |
| CCTGCACCACCATTACAAAC+TGG | + | chr4.1:14559249-14559268 | None:intergenic | 50.0% | |
| ! | CCAGTTTGTAATGGTGGTGC+AGG | - | chr4.1:14559246-14559265 | MS.gene09266:CDS | 50.0% |
| ! | GTTTGTAATGGTGGTGCAGG+TGG | - | chr4.1:14559249-14559268 | MS.gene09266:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 14559012 | 14559368 | 14559012 | ID=MS.gene09266 |
| chr4.1 | mRNA | 14559012 | 14559368 | 14559012 | ID=MS.gene09266.t1;Parent=MS.gene09266 |
| chr4.1 | exon | 14559012 | 14559368 | 14559012 | ID=MS.gene09266.t1.exon1;Parent=MS.gene09266.t1 |
| chr4.1 | CDS | 14559012 | 14559368 | 14559012 | ID=cds.MS.gene09266.t1;Parent=MS.gene09266.t1 |
| Gene Sequence |
| Protein sequence |
