AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035927

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035927.t1 MTR_5g062230 93.829 551 34 0 1 551 1 551 0.0 1077
MS.gene035927.t1 MTR_5g062230 91.837 98 8 0 654 751 456 553 1.41e-54 197
MS.gene035927.t1 MTR_5g062230 90.588 85 8 0 572 656 291 375 2.17e-43 165
MS.gene035927.t1 MTR_6g039440 75.681 551 134 0 1 551 1 551 0.0 891
MS.gene035927.t1 MTR_6g039440 80.612 98 19 0 654 751 456 553 5.95e-47 176
MS.gene035927.t1 MTR_6g039440 62.791 86 32 0 571 656 290 375 1.58e-28 121
MS.gene035927.t1 MTR_6g008560 71.892 555 152 2 1 551 1 555 0.0 849
MS.gene035927.t1 MTR_6g008560 80.612 98 19 0 654 751 460 557 8.44e-46 172
MS.gene035927.t1 MTR_6g008560 72.093 86 24 0 571 656 294 379 1.56e-32 133
MS.gene035927.t1 MTR_4g048460 69.104 547 166 1 8 551 13 559 0.0 784
MS.gene035927.t1 MTR_4g048460 71.429 98 28 0 654 751 464 561 5.51e-39 152
MS.gene035927.t1 MTR_4g048460 64.706 85 30 0 572 656 299 383 2.18e-22 102
MS.gene035927.t1 MTR_3g464190 66.547 556 182 2 1 552 1 556 0.0 780
MS.gene035927.t1 MTR_3g464190 69.388 98 30 0 654 751 460 557 3.58e-36 144
MS.gene035927.t1 MTR_3g464190 56.977 86 37 0 571 656 294 379 2.80e-27 117
MS.gene035927.t1 MTR_5g073200 65.741 540 184 1 13 551 8 547 0.0 753
MS.gene035927.t1 MTR_5g073200 65.306 98 34 0 654 751 452 549 9.24e-36 143
MS.gene035927.t1 MTR_5g073200 61.905 84 32 0 572 655 287 370 3.66e-23 104
MS.gene035927.t1 MTR_5g094620 63.718 554 179 2 1 551 1 535 0.0 747
MS.gene035927.t1 MTR_5g094620 68.367 98 31 0 654 751 440 537 5.14e-37 146
MS.gene035927.t1 MTR_5g094620 59.524 84 34 0 572 655 275 358 1.38e-26 115
MS.gene035927.t1 MTR_5g073260 62.749 553 201 3 1 551 1 550 0.0 733
MS.gene035927.t1 MTR_5g073260 66.327 98 33 0 654 751 455 552 2.67e-37 147
MS.gene035927.t1 MTR_5g073260 53.261 92 38 1 570 656 283 374 1.66e-24 108
MS.gene035927.t1 MTR_4g081460 55.154 553 242 3 2 549 7 558 0.0 642
MS.gene035927.t1 MTR_4g081460 49.495 99 50 0 653 751 464 562 5.70e-24 107
MS.gene035927.t1 MTR_4g081460 58.228 79 33 0 572 650 300 378 2.21e-22 102
MS.gene035927.t1 MTR_4g048460 68.805 452 138 1 8 456 13 464 0.0 642
MS.gene035927.t1 MTR_4g048460 64.706 85 30 0 572 656 299 383 1.42e-22 102
MS.gene035927.t1 MTR_6g008560 71.930 399 112 0 153 551 1 399 0.0 626
MS.gene035927.t1 MTR_6g008560 80.612 98 19 0 654 751 304 401 3.36e-47 173
MS.gene035927.t1 MTR_6g008560 72.093 86 24 0 571 656 138 223 2.16e-33 133
MS.gene035927.t1 MTR_2g081980 43.806 557 305 7 1 551 1 555 4.01e-162 480
MS.gene035927.t1 MTR_2g081980 47.222 72 38 0 572 643 294 365 1.16e-16 84.3
MS.gene035927.t1 MTR_2g081980 43.434 99 54 2 654 751 460 557 9.15e-14 75.1
MS.gene035927.t1 MTR_2g082010 43.911 542 300 4 13 551 26 566 7.52e-162 480
MS.gene035927.t1 MTR_2g082010 32.941 170 78 5 572 723 306 457 4.92e-19 92.0
MS.gene035927.t1 MTR_2g082010 45.455 99 52 2 654 751 471 568 2.19e-15 80.5
MS.gene035927.t1 MTR_8g007515 43.750 544 298 6 14 551 13 554 2.82e-160 476
MS.gene035927.t1 MTR_8g007515 42.697 89 49 1 569 655 290 378 1.31e-16 84.3
MS.gene035927.t1 MTR_8g007515 43.434 99 54 2 654 751 459 556 2.59e-14 77.0
MS.gene035927.t1 MTR_2g082060 42.908 557 298 9 1 551 9 551 1.59e-151 453
MS.gene035927.t1 MTR_2g082060 45.783 83 45 0 572 654 302 384 7.43e-17 85.1
MS.gene035927.t1 MTR_4g019550 42.806 556 299 8 1 548 9 553 3.70e-145 437
MS.gene035927.t1 MTR_4g019550 49.383 81 41 0 572 652 301 381 2.94e-18 89.4
MS.gene035927.t1 MTR_4g019550 38.384 99 59 2 654 751 461 558 2.15e-11 67.8
MS.gene035927.t1 MTR_2g082050 39.053 507 250 8 50 553 9 459 3.87e-116 358
MS.gene035927.t1 MTR_2g082050 40.206 97 56 2 654 749 362 457 8.45e-13 71.6
MS.gene035927.t1 MTR_4g045810 33.516 546 358 3 7 551 38 579 1.99e-106 337
MS.gene035927.t1 MTR_4g045810 43.210 81 46 0 573 653 320 400 3.02e-14 76.6
MS.gene035927.t1 MTR_2g064425 33.575 551 353 7 9 554 48 590 1.60e-94 306
MS.gene035927.t1 MTR_2g064425 46.914 81 43 0 573 653 326 406 1.36e-16 84.3
MS.gene035927.t1 MTR_2g089120 35.091 493 299 8 48 533 69 547 2.49e-93 302
MS.gene035927.t1 MTR_2g089120 48.000 75 39 0 577 651 309 383 1.19e-15 81.3
MS.gene035927.t1 MTR_2g089130 34.177 474 297 4 64 533 84 546 4.64e-91 296
MS.gene035927.t1 MTR_2g089130 43.038 79 45 0 577 655 309 387 1.51e-13 74.3
MS.gene035927.t1 MTR_7g057340 75.294 170 42 0 382 551 1 170 1.21e-87 273
MS.gene035927.t1 MTR_7g057340 78.571 98 21 0 654 751 75 172 1.59e-48 169
MS.gene035927.t1 MTR_8g057020 32.950 522 341 5 14 533 55 569 1.40e-86 285
MS.gene035927.t1 MTR_8g057020 41.026 78 46 0 577 654 332 409 2.58e-13 73.9
MS.gene035927.t1 MTR_2g064425 32.305 551 345 9 9 554 48 575 1.89e-86 284
MS.gene035927.t1 MTR_2g064425 45.783 83 45 0 573 655 311 393 1.29e-16 84.3
MS.gene035927.t1 MTR_2g065450 32.336 535 345 6 7 533 45 570 1.06e-85 283
MS.gene035927.t1 MTR_2g065450 48.148 81 42 0 573 653 330 410 1.25e-14 78.2
MS.gene035927.t1 MTR_4g092020 33.397 521 326 9 40 552 63 570 3.47e-81 270
MS.gene035927.t1 MTR_2g064425 40.489 368 210 5 9 374 48 408 7.33e-81 265
MS.gene035927.t1 MTR_2g064425 46.914 81 43 0 573 653 326 406 1.15e-16 83.6
MS.gene035927.t1 MTR_7g057390 71.176 170 46 1 382 551 1 167 1.34e-79 252
MS.gene035927.t1 MTR_7g057390 60.976 123 46 1 631 751 47 169 1.38e-42 152
MS.gene035927.t1 MTR_7g010710 29.167 552 382 6 2 549 5 551 1.93e-75 254
MS.gene035927.t1 MTR_7g010710 34.234 111 70 1 573 683 293 400 1.10e-11 68.6
MS.gene035927.t1 MTR_4g092010 29.466 543 372 7 12 549 20 556 3.09e-74 251
MS.gene035927.t1 MTR_4g092010 46.875 64 34 0 571 634 299 362 2.74e-11 67.4
MS.gene035927.t1 MTR_2g064425 38.587 368 202 7 9 374 48 393 6.62e-73 244
MS.gene035927.t1 MTR_2g064425 46.914 81 43 0 573 653 311 391 9.78e-17 84.0
MS.gene035927.t1 MTR_5g094530 55.046 218 62 3 163 378 1 184 1.46e-70 229
MS.gene035927.t1 MTR_5g094530 53.061 49 23 0 608 656 133 181 3.11e-11 63.5
MS.gene035927.t1 MTR_2g010960 27.885 520 358 8 38 543 1 517 9.99e-67 230
MS.gene035927.t1 MTR_6g064980 27.935 494 346 7 60 544 21 513 2.32e-57 204
MS.gene035927.t1 MTR_3g063170 27.004 474 338 7 60 526 21 493 1.02e-56 202
MS.gene035927.t1 MTR_3g063120 28.205 468 321 7 70 526 6 469 8.80e-56 199
MS.gene035927.t1 MTR_7g050990 56.989 186 62 4 189 370 16 187 1.05e-55 206
MS.gene035927.t1 MTR_7g050990 48.750 80 36 2 572 651 113 187 4.44e-16 83.2
MS.gene035927.t1 MTR_3g465090 27.677 495 346 8 60 544 21 513 1.59e-55 199
MS.gene035927.t1 MTR_3g052120 28.261 506 346 12 52 547 14 512 2.67e-55 198
MS.gene035927.t1 MTR_3g063140 26.721 494 352 8 60 544 21 513 4.56e-54 195
MS.gene035927.t1 MTR_6g065040 27.865 384 270 5 149 526 9 391 1.01e-46 171
MS.gene035927.t1 MTR_3g015080 47.647 170 51 2 382 551 1 132 1.20e-44 157
MS.gene035927.t1 MTR_3g015080 62.963 81 30 0 671 751 54 134 2.35e-29 114
MS.gene035927.t1 MTR_3g058160 28.241 432 271 14 66 467 64 486 2.80e-34 139
MS.gene035927.t1 MTR_3g058160 28.241 432 271 14 66 467 255 677 9.35e-34 139
MS.gene035927.t1 MTR_3g058160 28.241 432 271 14 66 467 268 690 1.10e-33 139
MS.gene035927.t1 MTR_3g058160 28.241 432 271 14 66 467 275 697 1.27e-33 139
MS.gene035927.t1 MTR_3g063160 30.112 269 184 4 60 325 21 288 2.56e-32 127
MS.gene035927.t1 MTR_3g058160 27.373 453 269 15 66 467 275 718 4.87e-31 130
MS.gene035927.t1 MTR_2g064295 26.494 385 257 9 66 427 255 636 1.98e-30 128
MS.gene035927.t1 MTR_2g012900 23.848 499 335 14 21 484 212 700 4.27e-29 124
MS.gene035927.t1 MTR_2g012870 23.941 472 322 14 21 468 212 670 1.19e-26 116
MS.gene035927.t1 MTR_7g094970 27.881 269 164 7 70 310 211 477 5.17e-22 101
MS.gene035927.t1 MTR_5g030050 28.302 265 155 8 70 309 3 257 1.08e-20 94.0
MS.gene035927.t1 MTR_7g011663 29.008 262 158 5 68 302 347 607 5.53e-20 95.5
MS.gene035927.t1 MTR_7g011770 29.210 291 177 6 70 332 245 534 5.84e-20 95.5
MS.gene035927.t1 MTR_5g081730 38.542 96 59 0 159 254 16 111 1.53e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035927.t1 AT5G23960 40.406 542 314 5 14 551 7 543 1.80e-145 437
MS.gene035927.t1 AT5G23960 45.918 98 52 1 654 751 449 545 6.22e-22 100
MS.gene035927.t1 AT5G23960 41.892 74 43 0 571 644 283 356 4.59e-12 69.7
MS.gene035927.t1 AT5G23960 40.441 544 313 6 14 551 7 545 3.39e-145 436
MS.gene035927.t1 AT5G23960 45.918 98 52 1 654 751 451 547 6.12e-22 100
MS.gene035927.t1 AT5G23960 42.105 76 42 1 571 644 283 358 6.63e-12 69.3
MS.gene035927.t1 AT1G70080 37.746 559 329 9 5 549 54 607 1.57e-122 380
MS.gene035927.t1 AT1G70080 31.387 137 86 2 572 707 348 477 1.68e-15 80.9
MS.gene035927.t1 AT3G14490 37.893 541 326 5 14 548 60 596 9.39e-119 370
MS.gene035927.t1 AT3G14490 38.356 73 45 0 572 644 339 411 8.19e-13 72.4
MS.gene035927.t1 AT3G14490 40.404 99 58 1 654 751 503 601 6.42e-12 69.3
MS.gene035927.t1 AT1G31950 34.857 525 332 5 19 537 70 590 8.65e-111 349
MS.gene035927.t1 AT1G31950 38.824 85 52 0 572 656 343 427 1.61e-16 84.3
MS.gene035927.t1 AT1G31950 34.791 526 332 6 19 537 70 591 6.46e-110 347
MS.gene035927.t1 AT1G31950 38.824 85 52 0 572 656 344 428 1.78e-16 84.0
MS.gene035927.t1 AT3G14520 34.637 537 339 6 19 548 68 599 9.47e-110 347
MS.gene035927.t1 AT3G14520 37.209 86 54 0 572 657 341 426 3.59e-14 76.6
MS.gene035927.t1 AT3G29410 35.338 532 337 5 14 541 64 592 2.87e-108 343
MS.gene035927.t1 AT3G29410 33.735 83 55 0 572 654 341 423 9.86e-11 65.5
MS.gene035927.t1 AT5G44630 35.283 547 341 9 15 552 14 556 5.33e-108 340
MS.gene035927.t1 AT5G44630 35.000 100 63 1 652 749 456 555 1.13e-11 68.6
MS.gene035927.t1 AT3G14540 34.823 537 338 6 19 548 65 596 3.15e-107 340
MS.gene035927.t1 AT3G14540 30.128 156 94 3 572 715 338 490 4.53e-11 66.6
MS.gene035927.t1 AT1G33750 34.393 535 335 8 13 540 65 590 9.59e-106 336
MS.gene035927.t1 AT4G15870 35.581 534 320 10 24 549 77 594 3.84e-104 332
MS.gene035927.t1 AT4G15870 35.632 87 55 1 572 657 347 433 8.88e-12 68.9
MS.gene035927.t1 AT4G13300 35.701 535 333 7 24 551 22 552 1.84e-103 328
MS.gene035927.t1 AT4G13300 46.341 82 43 1 669 749 471 552 2.84e-12 70.5
MS.gene035927.t1 AT3G29410 36.307 482 301 4 56 534 1 479 9.67e-103 325
MS.gene035927.t1 AT3G32030 34.637 537 339 8 19 548 68 599 1.26e-102 328
MS.gene035927.t1 AT4G20230 34.727 550 343 10 12 551 60 603 2.92e-101 324
MS.gene035927.t1 AT4G20230 39.080 87 50 2 572 656 341 426 4.00e-11 67.0
MS.gene035927.t1 AT4G20230 34.727 550 343 10 12 551 72 615 3.61e-101 325
MS.gene035927.t1 AT4G20230 39.080 87 50 2 572 656 353 438 4.36e-11 66.6
MS.gene035927.t1 AT4G20210 32.907 547 358 6 8 548 52 595 1.13e-99 320
MS.gene035927.t1 AT4G13280 35.821 536 331 8 24 551 22 552 9.45e-98 313
MS.gene035927.t1 AT4G13280 45.238 84 45 1 669 751 471 554 2.80e-12 70.5
MS.gene035927.t1 AT4G20200 33.333 546 351 7 14 551 62 602 3.04e-97 314
MS.gene035927.t1 AT4G20200 27.179 195 105 7 589 751 415 604 1.96e-11 67.8
MS.gene035927.t1 AT3G29190 33.895 534 344 6 24 551 69 599 6.37e-95 308
MS.gene035927.t1 AT3G29190 33.333 93 62 0 659 751 509 601 7.02e-11 66.2
MS.gene035927.t1 AT2G23230 32.240 549 357 9 13 551 55 598 1.75e-92 301
MS.gene035927.t1 AT1G31950 34.685 444 280 5 19 456 70 509 2.62e-92 298
MS.gene035927.t1 AT1G31950 38.824 85 52 0 572 656 344 428 1.38e-16 84.0
MS.gene035927.t1 AT2G23230 32.130 554 346 10 13 551 55 593 4.63e-91 297
MS.gene035927.t1 AT1G66020 34.004 547 346 9 14 551 56 596 5.80e-91 297
MS.gene035927.t1 AT3G29110 33.211 545 353 7 14 551 55 595 5.31e-90 294
MS.gene035927.t1 AT4G16730 31.478 521 341 6 43 551 21 537 1.41e-88 289
MS.gene035927.t1 AT4G16730 36.709 79 50 0 573 651 275 353 2.76e-12 70.5
MS.gene035927.t1 AT3G14540 34.821 448 281 5 19 460 65 507 2.82e-88 287
MS.gene035927.t1 AT3G14540 30.128 156 94 3 572 715 338 490 3.73e-11 66.6
MS.gene035927.t1 AT4G16730 31.781 494 322 5 70 551 1 491 2.21e-86 281
MS.gene035927.t1 AT4G16730 36.709 79 50 0 573 651 229 307 2.33e-12 70.5
MS.gene035927.t1 AT4G13280 34.515 536 308 10 24 551 22 522 5.87e-86 281
MS.gene035927.t1 AT4G13280 28.632 234 113 7 572 751 291 524 8.77e-13 72.0
MS.gene035927.t1 AT1G48800 31.843 559 364 10 2 549 50 602 3.56e-85 282
MS.gene035927.t1 AT4G16740 31.285 537 344 10 7 532 20 542 5.81e-81 270
MS.gene035927.t1 AT4G16740 44.444 63 35 0 573 635 306 368 1.92e-11 67.8
MS.gene035927.t1 AT3G25810 30.586 546 357 8 3 532 36 575 2.05e-79 266
MS.gene035927.t1 AT3G25810 43.373 83 47 0 573 655 330 412 1.51e-13 74.7
MS.gene035927.t1 AT1G61680 31.548 504 325 8 52 547 78 569 3.89e-79 265
MS.gene035927.t1 AT2G24210 29.944 531 354 9 13 532 45 568 2.26e-78 263
MS.gene035927.t1 AT2G24210 36.905 84 53 0 572 655 324 407 5.01e-12 69.7
MS.gene035927.t1 AT3G25830 29.350 569 374 12 3 551 32 592 2.67e-77 261
MS.gene035927.t1 AT3G25830 38.554 83 51 0 573 655 328 410 1.57e-11 68.2
MS.gene035927.t1 AT3G25820 29.350 569 374 12 3 551 32 592 2.67e-77 261
MS.gene035927.t1 AT3G25820 38.554 83 51 0 573 655 328 410 1.57e-11 68.2
MS.gene035927.t1 AT1G61680 31.746 504 317 9 52 547 78 562 2.46e-76 257
MS.gene035927.t1 AT1G48820 31.123 543 322 12 14 549 63 560 5.33e-76 256
MS.gene035927.t1 AT5G48110 36.982 338 208 5 213 549 2 335 4.66e-72 238
MS.gene035927.t1 AT5G48110 39.535 86 51 1 572 657 81 165 2.29e-12 69.7
MS.gene035927.t1 AT4G16740 34.204 383 231 8 7 378 20 392 3.52e-65 222
MS.gene035927.t1 AT4G16740 44.444 63 35 0 573 635 306 368 1.52e-11 67.4
MS.gene035927.t1 AT1G66020 36.413 368 223 7 14 374 56 419 1.03e-63 219
MS.gene035927.t1 AT3G25820 32.080 399 248 9 3 386 32 422 1.71e-60 211
MS.gene035927.t1 AT3G25820 38.554 83 51 0 573 655 328 410 1.84e-11 67.4
MS.gene035927.t1 AT4G16740 35.542 332 193 8 7 327 20 341 6.64e-56 196
MS.gene035927.t1 AT4G16740 35.542 332 193 8 7 327 20 341 6.64e-56 196
MS.gene035927.t1 AT3G25820 32.647 340 206 9 3 327 32 363 5.83e-51 183
MS.gene035927.t1 AT3G25820 32.647 340 206 9 3 327 32 363 5.83e-51 183
MS.gene035927.t1 AT1G79460 24.277 519 346 19 66 551 273 777 1.38e-24 110
MS.gene035927.t1 AT1G61120 23.092 511 358 15 65 551 283 782 7.85e-21 98.6
MS.gene035927.t1 AT4G02780 29.323 266 158 6 68 305 332 595 4.80e-20 95.9

Find 119 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCTCTCGCTGTGCTATTT+AGG 0.126479 5.3:+49138840 MS.gene035927:CDS
AGATGTGTTCACCAAGTTTA+AGG 0.128999 5.3:+49150400 MS.gene035927:intron
AGATGTGTTCACCAAGTTTA+AGG 0.171551 5.3:+49139155 MS.gene035927:intron
GACTACATGAAATTAATTTA+TGG 0.181873 5.3:+49151235 MS.gene035927:CDS
CACAAGAGCTATGTTGAAAA+TGG 0.199028 5.3:+49138791 MS.gene035927:CDS
CTCTCTCGCTGTGCTATTTA+GGG 0.207543 5.3:+49138841 MS.gene035927:CDS
TATTCATGGAGAGGAAATTT+TGG 0.247279 5.3:+49139257 MS.gene035927:CDS
AGCCAATCTTTGCAGGTTAA+TGG 0.266883 5.3:+49145856 MS.gene035927:CDS
CAGAATTTCACCCAAATGTC+TGG 0.305980 5.3:+49138456 MS.gene035927:CDS
AAAACATGTTTGCTCATTGT+TGG 0.311477 5.3:+49146495 MS.gene035927:CDS
GGATTGTCGAATGTTGCTTC+TGG 0.320132 5.3:+49139688 MS.gene035927:CDS
AAATATTCTCCCCAGACATT+TGG 0.320286 5.3:-49138467 None:intergenic
GTGCCGCAGCCAATCTTTGC+AGG 0.328094 5.3:+49145849 MS.gene035927:CDS
ATTTCCTTGCTAGAGAATAC+TGG 0.329894 5.3:-49139734 None:intergenic
TAAATTAATTTCATGTAGTC+TGG 0.356370 5.3:-49140003 None:intergenic
AGATTTCTTTGCTTGGCGTC+AGG 0.356623 5.3:-49138431 None:intergenic
TAAATTAATTTCATGTAGTC+TGG 0.358352 5.3:-49151232 None:intergenic
ATCCATTAACCTGCAAAGAT+TGG 0.365552 5.3:-49145858 None:intergenic
AATATTCTCCCCAGACATTT+GGG 0.380119 5.3:-49138466 None:intergenic
AAGAAAAGGCATTGGAACTT+TGG 0.385858 5.3:-49146626 None:intergenic
AAGAAAAGGCATTGGAACTT+TGG 0.385858 5.3:-49153382 None:intergenic
CCACGGAGAACATCTTCAAT+TGG 0.392535 5.3:+49145801 MS.gene035927:CDS
TGCATGTACTCTTCTATAGT+TGG 0.397995 5.3:-49145710 None:intergenic
GCCTCACATATGATTATTCA+TGG 0.403293 5.3:+49139243 MS.gene035927:CDS
CAGGTGGGATATTAGCATCT+TGG 0.406616 5.3:+49139972 MS.gene035927:intron
CAGGTGGGATATTAGCATCT+TGG 0.406616 5.3:+49151201 MS.gene035927:intron
ATATAAATGAGGAATGTCTT+AGG 0.410838 5.3:+49146599 MS.gene035927:CDS
ATATAAATGAGGAATGTCTT+AGG 0.410838 5.3:+49153355 MS.gene035927:CDS
AAGCAGTTGCAATTCCCTTC+TGG 0.421915 5.3:-49146570 None:intergenic
AAGCAATTGCAATTCCCTTC+TGG 0.422993 5.3:-49153326 None:intergenic
TTCTGCAAGATTTCTTTGCT+TGG 0.427036 5.3:-49138438 None:intergenic
TTAACTTCTTACATTGGAAT+GGG 0.433315 5.3:+49145770 MS.gene035927:CDS
GAGCAGGAAATGAGGAAAGA+AGG 0.444481 5.3:+49140057 MS.gene035927:CDS
AGAAGGGAATTGCAATTGCT+TGG 0.448404 5.3:+49153328 MS.gene035927:CDS
GAGCAGGAAATGAGGAAAGA+AGG 0.449185 5.3:+49151286 MS.gene035927:CDS
CTAGCCTTGGCAAGTTCTTG+TGG 0.451175 5.3:-49139380 None:intergenic
ATTAACTTCTTACATTGGAA+TGG 0.453994 5.3:+49145769 MS.gene035927:CDS
GACAAACCTTTGAATAGCCT+TGG 0.454010 5.3:-49151075 None:intergenic
CCTTCATAAGTCCTTGAGAA+TGG 0.454397 5.3:-49146711 None:intergenic
TCCTTCCTCGTCCTTAAACT+TGG 0.460450 5.3:-49139166 None:intergenic
TCCTTGCTCATCCTTAAACT+TGG 0.463698 5.3:-49150411 None:intergenic
TGCAAGAAAGTGACTCGTTT+GGG 0.467677 5.3:-49139325 None:intergenic
AAAGGCATTGGAACTTTGGT+TGG 0.468298 5.3:-49146622 None:intergenic
AAAGGCATTGGAACTTTGGT+TGG 0.468298 5.3:-49153378 None:intergenic
GATGATACATATGATGCATA+TGG 0.472052 5.3:+49139792 MS.gene035927:CDS
GATGATACATATGATGCATA+TGG 0.472052 5.3:+49151026 MS.gene035927:CDS
TTAAAGATGGTGGAAGGAGT+TGG 0.472988 5.3:+49139632 MS.gene035927:intron
AACTGCCTTATGCACGTCAT+AGG 0.477010 5.3:+49139667 MS.gene035927:CDS
TGATGAAATTCTACTCACTT+TGG 0.477899 5.3:+49139446 MS.gene035927:CDS
TGAAGGAGTATGATATGTCT+AGG 0.485271 5.3:+49146527 MS.gene035927:CDS
TGAAGCAGTATGATATGTCT+AGG 0.485271 5.3:+49153283 MS.gene035927:CDS
ACAATCCTATGACGTGCATA+AGG 0.495156 5.3:-49139672 None:intergenic
CTGCAAGAAAGTGACTCGTT+TGG 0.497922 5.3:-49139326 None:intergenic
TTGATATTAACTTCTTACAT+TGG 0.498156 5.3:+49145764 MS.gene035927:CDS
GAACAACCTTTGAATTGCCT+TGG 0.501955 5.3:-49139841 None:intergenic
AGAAGGGAATTGCAACTGCT+TGG 0.502158 5.3:+49146572 MS.gene035927:CDS
TTTCCTTGCTAGAGAATACT+GGG 0.503157 5.3:-49139733 None:intergenic
AAGAAGTAGAGCAGGAAATG+AGG 0.507319 5.3:+49151278 MS.gene035927:CDS
CACGGAGAACATCTTCAATT+GGG 0.510447 5.3:+49145802 MS.gene035927:CDS
GTGACTCGTTTGGGTGGCAA+TGG 0.514494 5.3:-49139316 None:intergenic
CTTCATAAGTCCTTGAGAAT+GGG 0.514640 5.3:-49146710 None:intergenic
TTGAGAGCACGAATAAGAAA+AGG 0.516227 5.3:-49146640 None:intergenic
TTGAGAGCACGAATAAGAAA+AGG 0.516227 5.3:-49153396 None:intergenic
AATTAATTTATGAAACACTC+TGG 0.517037 5.3:+49140016 MS.gene035927:CDS
GATTTCTTTGCTTGGCGTCA+GGG 0.518796 5.3:-49138430 None:intergenic
AAGAAATAGAGCAGGAAATG+AGG 0.520129 5.3:+49140049 MS.gene035927:CDS
AGAATTTCACCCAAATGTCT+GGG 0.526240 5.3:+49138457 MS.gene035927:CDS
AGTCAAATATGCTTTAAGAC+AGG 0.532235 5.3:+49139353 MS.gene035927:CDS
AATTAATTTATGGAACACTC+TGG 0.533198 5.3:+49151245 MS.gene035927:CDS
TATCGTCGTGCCTCTAGCCT+TGG 0.535193 5.3:-49139393 None:intergenic
ATATAATTTAAATACCTCGC+TGG 0.542149 5.3:-49145891 None:intergenic
CAGATAAAAGTGACAGTCCA+AGG 0.542805 5.3:+49145647 MS.gene035927:intron
ATTGTTGGATTGCTACATGA+AGG 0.548102 5.3:+49146510 MS.gene035927:CDS
TGATCTTTAAAGATGGTGGA+AGG 0.548329 5.3:+49139626 MS.gene035927:intron
ACGACATATTGAATCAATCA+AGG 0.552162 5.3:-49138714 None:intergenic
ACATTCTGAAGGACTTATGA+AGG 0.556220 5.3:+49153467 MS.gene035927:CDS
ATAGCACAGCGAGAGAGTAA+AGG 0.556765 5.3:-49138835 None:intergenic
GACAATTACACACATTCTGA+AGG 0.560278 5.3:+49153456 MS.gene035927:CDS
GACAATTACACCCATTCTCA+AGG 0.561159 5.3:+49146700 MS.gene035927:CDS
AGCCCCAGTATTCTCTAGCA+AGG 0.562995 5.3:+49139730 MS.gene035927:CDS
TGGCCTCAGTAATAAAACCT+TGG 0.569302 5.3:-49145664 None:intergenic
GAACTAATCACCGATGTCGA+AGG 0.571062 5.3:+49139201 MS.gene035927:CDS
GCTTAACATACCTTCGACGT+TGG 0.572942 5.3:-49150456 None:intergenic
ATTGATTCAATATGTCGTCT+CGG 0.576187 5.3:+49138719 MS.gene035927:CDS
TCAATATGCTTCAGAATCGA+TGG 0.579838 5.3:+49138493 MS.gene035927:CDS
CCATTCTCAAGGACTTATGA+AGG 0.579997 5.3:+49146711 MS.gene035927:CDS
AACTAATCACCGATGTCGAA+GGG 0.581512 5.3:+49139202 MS.gene035927:CDS
ACCAAGTTTAAGGATGAGCA+AGG 0.581888 5.3:+49150410 MS.gene035927:CDS
GCACGAATAAGAAAAGGCAT+TGG 0.582827 5.3:-49146634 None:intergenic
GCACGAATAAGAAAAGGCAT+TGG 0.582827 5.3:-49153390 None:intergenic
GCTTAACATCCCTTCGACAT+CGG 0.583023 5.3:-49139211 None:intergenic
CAAGAACTTGCCAAGGCTAG+AGG 0.583333 5.3:+49139383 MS.gene035927:CDS
CTCTCCACAAGAACTTGCCA+AGG 0.588910 5.3:+49139376 MS.gene035927:CDS
ACCAAGTTTAAGGACGAGGA+AGG 0.589430 5.3:+49139165 MS.gene035927:CDS
GAAGGAGTATGATATGTCTA+GGG 0.592618 5.3:+49146528 MS.gene035927:CDS
GTTCACCAAGTTTAAGGACG+AGG 0.597717 5.3:+49139161 MS.gene035927:CDS
GGCAATGGACTCGAGGTGAG+TGG 0.598560 5.3:-49139301 None:intergenic
GAAGCAGTATGATATGTCTA+GGG 0.603557 5.3:+49153284 MS.gene035927:CDS
AGTAGAATTTCATCATGCGA+AGG 0.604328 5.3:-49139438 None:intergenic
TGCTATTCAAGAATGCCAGA+AGG 0.606199 5.3:+49146555 MS.gene035927:CDS
TGCTATTCAAGAATGCCAGA+AGG 0.606199 5.3:+49153311 MS.gene035927:CDS
TAACCTGCAAAGATTGGCTG+CGG 0.606263 5.3:-49145852 None:intergenic
GCTATTCAAGAATGCCAGAA+GGG 0.608852 5.3:+49146556 MS.gene035927:CDS
GCTATTCAAGAATGCCAGAA+GGG 0.608852 5.3:+49153312 MS.gene035927:CDS
AGACTAATCACCAACGTCGA+AGG 0.613335 5.3:+49150446 MS.gene035927:CDS
TTTGGGTGGCAATGGACTCG+AGG 0.626346 5.3:-49139308 None:intergenic
TGCTTGGAAAGATATAAATG+AGG 0.627963 5.3:+49146588 MS.gene035927:CDS
TGCTTGGAAAGATATAAATG+AGG 0.630445 5.3:+49153344 MS.gene035927:CDS
ACATATGATTATTCATGGAG+AGG 0.630498 5.3:+49139248 MS.gene035927:CDS
AAGAAAGTGACTCGTTTGGG+TGG 0.638271 5.3:-49139322 None:intergenic
TCCATGAATAATCATATGTG+AGG 0.649430 5.3:-49139244 None:intergenic
CCAATTGAAGATGTTCTCCG+TGG 0.681269 5.3:-49145801 None:intergenic
GAATTTCACCCAAATGTCTG+GGG 0.686951 5.3:+49138458 MS.gene035927:CDS
TTCCTTGCTAGAGAATACTG+GGG 0.699519 5.3:-49139732 None:intergenic
ATACTGGGGCTCAAAATATG+CGG 0.703279 5.3:-49139718 None:intergenic
GGATGAAATTGTCTCCAGCG+AGG 0.739710 5.3:+49145877 MS.gene035927:CDS
AGATTGGAACACGATCAACA+AGG 0.741051 5.3:-49153506 None:intergenic
AAATTGGAACACGATCAACA+AGG 0.741392 5.3:-49146750 None:intergenic
TGGAATGGGAGAAAATACCA+CGG 0.766243 5.3:+49145784 MS.gene035927:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.3 gene 49138407 49153530 49138407 ID=MS.gene035927
chr5.3 mRNA 49138407 49153530 49138407 ID=MS.gene035927.t1;Parent=MS.gene035927
chr5.3 exon 49138407 49138514 49138407 ID=MS.gene035927.t1.exon1;Parent=MS.gene035927.t1
chr5.3 CDS 49138407 49138514 49138407 ID=cds.MS.gene035927.t1;Parent=MS.gene035927.t1
chr5.3 exon 49138617 49138896 49138617 ID=MS.gene035927.t1.exon2;Parent=MS.gene035927.t1
chr5.3 CDS 49138617 49138896 49138617 ID=cds.MS.gene035927.t1;Parent=MS.gene035927.t1
chr5.3 exon 49139157 49139529 49139157 ID=MS.gene035927.t1.exon3;Parent=MS.gene035927.t1
chr5.3 CDS 49139157 49139529 49139157 ID=cds.MS.gene035927.t1;Parent=MS.gene035927.t1
chr5.3 exon 49139638 49139856 49139638 ID=MS.gene035927.t1.exon4;Parent=MS.gene035927.t1
chr5.3 CDS 49139638 49139856 49139638 ID=cds.MS.gene035927.t1;Parent=MS.gene035927.t1
chr5.3 exon 49139975 49140113 49139975 ID=MS.gene035927.t1.exon5;Parent=MS.gene035927.t1
chr5.3 CDS 49139975 49140113 49139975 ID=cds.MS.gene035927.t1;Parent=MS.gene035927.t1
chr5.3 exon 49145650 49145898 49145650 ID=MS.gene035927.t1.exon6;Parent=MS.gene035927.t1
chr5.3 CDS 49145650 49145898 49145650 ID=cds.MS.gene035927.t1;Parent=MS.gene035927.t1
chr5.3 exon 49146481 49146760 49146481 ID=MS.gene035927.t1.exon7;Parent=MS.gene035927.t1
chr5.3 CDS 49146481 49146760 49146481 ID=cds.MS.gene035927.t1;Parent=MS.gene035927.t1
chr5.3 exon 49150402 49150467 49150402 ID=MS.gene035927.t1.exon8;Parent=MS.gene035927.t1
chr5.3 CDS 49150402 49150467 49150402 ID=cds.MS.gene035927.t1;Parent=MS.gene035927.t1
chr5.3 exon 49150982 49151090 49150982 ID=MS.gene035927.t1.exon9;Parent=MS.gene035927.t1
chr5.3 CDS 49150982 49151090 49150982 ID=cds.MS.gene035927.t1;Parent=MS.gene035927.t1
chr5.3 exon 49151204 49151342 49151204 ID=MS.gene035927.t1.exon10;Parent=MS.gene035927.t1
chr5.3 CDS 49151204 49151342 49151204 ID=cds.MS.gene035927.t1;Parent=MS.gene035927.t1
chr5.3 exon 49153237 49153530 49153237 ID=MS.gene035927.t1.exon11;Parent=MS.gene035927.t1
chr5.3 CDS 49153237 49153530 49153237 ID=cds.MS.gene035927.t1;Parent=MS.gene035927.t1
Gene Sequence

>MS.gene035927

ATGTCTACTGTAGCTTACTCTAACCCTGACGCCAAGCAAAGAAATCTTGCAGAATTTCACCCAAATGTCTGGGGAGAATATTTCATTCAATATGCTTCAGAATCGATGGTAAATATATATTGCTATTTCCACTAATTGGTTATGATATTATAAATGTTCCATCCATTATTCGAGCAAACGGTTAACCAGCTTATCTCTAATCATATGCAGGAACTTGATCAGAATATTGCAGCACAAATCGTTAAATTAAAAGATGAAGTGAGAAACATGCTTGTCTCAAAAACTGAGATGCCATTGGCAAAAGTTACCTTGATTGATTCAATATGTCGTCTCGGTGTTAGTTATCATTTTGAAAACGAGATTGACGAACTTTTGCAACATTTTCACAAGAGCTATGTTGAAAATGGAGAAATAGTTCTTGAAGACAGCCTTTACTCTCTCGCTGTGCTATTTAGGGTTTTGAGGCAACAAGGATTTTATGTTTCACCAAGTATATATCCATCCATGACTCAAACATATTTTATTTACATTTTTTTATTTCTTAATAAAAAAAAAGATGTGAAAAACACTAAGATTATGATTTGTAAAAAAAAAAACAGTGAATAACCGATAAGTTAATTGGTTGAATTTTTAATGTTTTATAAAATTAACGGTTGAACTAGTTTATAAATATGAAATGATATAAAAATATTTAATTTTTTTTATGCGACTAATGATTGTTAATTTTTTTTTTTTTTATAAACACACTAGATGTGTTCACCAAGTTTAAGGACGAGGAAGGAAACTTTAATAAAGAACTAATCACCGATGTCGAAGGGATGTTAAGCTTGTATGAAGCCTCACATATGATTATTCATGGAGAGGAAATTTTGGAAGAGGCATTGGCTTTTACGTCCACTCACCTCGAGTCCATTGCCACCCAAACGAGTCACTTTCTTGCAGCACAAGTCAAATATGCTTTAAGACAGGCTCTCCACAAGAACTTGCCAAGGCTAGAGGCACGACGATACATTTCTATTTATGAACAAAATCCTTCGCATGATGAAATTCTACTCACTTTGGCGAAATTAGATTTTAATTTGCTCCAAAGCCTACATCAAAAAGAGTTTGGAAACATTTGCAAGTAAGTACTTCAATCATACACTTCGATTCTTTGTAACAAAACAAAATTAAAGTTATCATAGGATAAATCCTGTTAATAACTTTTTTATATTGATTTTGATCTTTAAAGATGGTGGAAGGAGTTGGACTTCTCTAGTAAACTGCCTTATGCACGTCATAGGATTGTCGAATGTTGCTTCTGGATTTTGACCGCATATTTTGAGCCCCAGTATTCTCTAGCAAGGAAATTAGCAACGAAAGTAATCGCGATGTTATCAATCATTGATGATACATATGATGCATATGGAACCATTGATGAACTAGAACTTTTTACCAAGGCAATTCAAAGGTTGTTCATCATAAATTTATTAAACAAAAGAACTGGAACATATATTTACATTTCTCTCTTGCTTATAAAAAATATTTTTCCCTATATTTAAAAATAAAATGTTAAAAGTGATTTTCAGGTGGGATATTAGCATCTTGGATGATCTTCCAGACTACATGAAATTAATTTATGAAACACTCTGGAATATTTTCGAAGAAATAGAGCAGGAAATGAGGAAAGAAGGAAAGCTTTATACCATGAAGTACTTTATAAAAGAAGTATGAGTTTGTGCCTTCTCTAATTAATTATTAGCAACAAAACATGAGCCATAACTTTTTTTTCTTCTACCAATTGAGTTAAGCTCACGAGGACATCCATAACTTATTTATACATATAAGTATCCTTTTTATAAGAGCAAAGATAATTTATATTAATAAGAACAATCAATAACAACACAAAATTGTATTGATTTTAATTATACTCTTTTGTCTTAACCCTCATATTCTTAGGGGGATAGTGAGTTGATAATCAAGAGCTGATTAAGAATTGCAGATTATTAAAGAGGATTTCGAAGGATTCCTCCTTAAATCACTCTCATTAATCACTTAAGACCACACAATTAATGAAAGGGAAATGTTAATAGATGCATTTGGGATATTGTTTAAGCAACTAAAAATAGTAATTTTTGCATAGAAATTATTGTGTACAATTATTAGTTTTACATGTGTTTGACTTATGTTTTTAAGATAAAATTTCTTTTAATGGATTCCTTAAACAATATCCTTAGGGCACTTGTTAGCATAACTCTTAATTAAATGTTTCTCCTTTCTTTAACTACAAGTGGTTGAATTTGCTTAACTCAACTAGTATTAAACATGGTTACGTTGTCATTTATGTTTGTTTCTTTTTAACGCAGTCATTTATGTTTCTTAAATATTGAATGTCGTTAGTTTAGCCTTTTAGGAGTGAAAGTTCATGCAAAAGTTTAGGAGCAAACTCTCTCAGCCTTTATTTGGTTGGAAACAAATCAAATTTTAAATTTGTGAAGGAATTTTTGTTACACAAAAATTGTGATGTTAAGAAACTTAACATTACTTTTGTTAAATATCATGCTTAGGACTGCTGATGTGAAGACTTTTATGTATTATGTGTTGGTTATTTTATTTTAGTAACTGCTGTTCAGTTTTCTTTAATTAATTGTGTTGTATTGTGGTAGTTATTGGAAGTTTTTAGTATTGTCAAACTATGTAAAACATAGTGTGTGTTCTGTTTGTTTTTTATTAGACAAATTATATGAATGAAAGCAGTTATTTCTCCTTTGTCTTGTTCATTTCCTGCAACTGTTTTCTTGTTCAATTAACAACAATTGGTATCTAGAGCCCTATTCTAAGGGATCTGTGTGAGTTTAACGTCATGGAAACCGAAGCAAGTCTCTCCGGCATCGCTCCGACGGTGTTCGACGGAAGTAACTACCAACTTTGGGCAGTGCGTATGGAGGCATATTTGGAAGCTTTGGATTTGTGGGAAGCAGTGGAAGAAGATTATGAAATTCCTGTACTTCCAAACAATCCCACCATGGCACAAATAAAAATGCAAAAGGAAAAAAAGACAAAGAAGGCAAAGGCAAAGGCCTGCTTGTTTGCTGCTGTCTCAACCACTATTTTCACAAGAATCATGTCCTTGAAATCAGCAAAGGAGATATGGGATTATCTCAAGAAAGAATATGAAGGTGATGAAAGAATTAAAGGCATGCAAGTCCTGAACCTGATCCGGGAATTCGAACTTCAAAGAATGAAGGATTCTGAGACGATTCAAGATTACTCTGACAAACTTCTCAACATTGCAAACAATGTGAGACTTTTGGGATCAGAATTCTCTGACTCAAGAATTGTTGAAAAAATTCTTGTAACGGTACCCGAAAGATATGAAGCAACCATCACTACTTTGGAGAATACCAAAGATTTCTCAAAAATTTCTTTGGCAGAATTGCTTAATGCATTGAAGGCACAAGAACAGAGAAGATTGATGAGAGAGGAAACAACACTTGAAGGAGCATTAACAGCCAAGCAGGATAATGTGGGATCAACATCAGCCAATGCGACAGAAAAGAATGTAAATCGAAGAAAATCTTATCCTCCATGTCAACATTGTGGAAAGAAAGGCCATCCACCCTTTAGATGTTGGAGAAGACCTGATGCTAAGTGTACCAAGTGTAATCAAATGGGACATGAAGTTGTTATTTGTAAAAATAGGAATCAACAACATAATGATGAGGCAAAGGTTGTTGATCAAGAGGAAGAGGAGGATCACTTATTCGTTGCAACATGTTTCTCTAGCATCGAATCAAGTGAGAATTGGCTGATTGATAGTGGTTGTACCAACCACATGACACATAACAAAGATTTATTCAGGGAACTAAACAATGCAAACTCAACAAAAGTTCGGGTTGGAAATGGCAATTACATTGCTGTGAAAGGAAAAGGAACAATAGCAATATCAACCTATTCAGGTACAAAGTTAATTTCTGATGTGTTGTACGTCCCTGAAATTGATCAGAATTTGTTAAGTGTTGGACAATTAATTGAAAGAGGTTATAAAGTGTTGTTTGAAAATGAAAGCTGCTTGATTAAAGATAGTGATGGCAAAGACATTTTCAAAGTAAAAATGAGAGGAAAAAGTTTTGCTCTAAATCCATTGAAGGAGGAGCAAATTGCTTTTCAAATGAAAGAAAATGTCACAGACTTATGGCACAAAAGGCTCGGACATTATCATCATCAAGGTCTGCTGCAGTTGAAATCCAAGGAGATGGCAAATGACATTCCAGAACTTGATGACCATATTTCAAATTGCAAGGCTTGTCAGTTTGGAAAGCAAAGCAGGAAACCATTTCCAAAGACAACTTGGAGAGCAACAAAGAAGTTGCAATTAATTCATACTGACATTGCAGGCCCACAAAGAACATCTTCTTTGAATGGTAGTCTTTATTATGCTATATTCATTGATGATTTTACTCGTATGTGTTGGATCTTCTTTCTGAAGCACAAGTCAGAGGTAGCACAAGTATTTTGGAAATTCAAAGCAAGAGTGGAGAATGAAAGTGGTTGTAAAATTCAAGCTCTCAGATCAGACAATGGCAAAGAGTATACTTCTGAAGCATTTGATCGATTCTGTGACGACGCTGGCATTCATCATCAGTTGACTGTTCCATACACTCCACAACAAAATGGAGTTAGTGAAAGAAGAAACAGATTTATATTGGAGATGACCCGATGTATGCTGCATGAGAAGAACTTGCCAAAGCAATTTTGGGCGGAATCGGCAAACACTGCTGTTTATTTGCAAAATAGGCTTCCTACAAGAGCAGTAAAGGATAAGACGCCATTTGAGGCTTGGTATGGTTATAAACCCTCCCTGAATTTCCTTAAAATATTTGGATGTTTGTGCTTTACTCATGTTCCACAAATCAAGAGAGATAAACTAGACAAAAGAGCTCTACCAGGCATTTTTATTGGCTACAGCTCCGTTGCAAAAGCTTATAAAATTTTCCAGCCACAAAATGGTAAAATTATTGTAAGTAGAGATGTGCATTTTATGGAAGATGAAGAGTGGAACTGGGATGACACAAAGGAGATGGAGGTAATTTCTAAAAATCCAAAACTCCAGATTGATGAGAACATTGAAGATTGGGAAAATGAAATGGTAGATGACGTTCCTGTCAGGGGAACAAGATTACTTTCTGATGTTTATCAAAGATGCAACATTGCTGTTTGTGAGCCTACTGGTTTTGAAGAAGCAAAACTTGATAAAAATTGGATGGTTGCAATGGAGGAGGAGTTGCACATGATTGAGAAAAATAATACATGGCAGCTTGTTGACAGACCTCAAGATAGGAAAGTCATAGGCGTCAAGTGGGTTTTCAAAACTAAGCTCAATGCTGATGGCTCTATCAACAAGTTCAAGGCAAGGCTAGTTGTTAAAGGTTATGCTCAGATTTTTGGAGTGGACTACTCTGATACATTTGCGCCAGTGGCCAGGCTAGACACCATCAGGATGCTACTTGCTGTTGCAGCCCAAAAGGGTTGGAAAGTATACCAGTTGGATGTTAAATCAGCTTTTTTAAATGGTTTCTTGCAAGAAGAAATTTATGTTGAACAGCCTGAAGGCTTTATGAAGAAAGGGGAAGAAGACAAGGTTTATCTTCTTAAGAAGGCTCTTTATGGATTGAAGCAGGCACCAAGAGCTTGGTACAGCAGAATTGATAATCATCTGCTGACTTTGGGCTTTGTGAGAAGCTTGTCTGAGTCTACCCTTTACATCAAACACAATGAGGTTGATATTCTTGTTGTTTCTCTCTATGTTGATGATATTTTGGTGACAGGAAATAATGCAACATTGATTGATGAATTTAAATTGGAAATGATGAAGGAATTTGAGATGACAGATCTTGGATTAATGACTTATTTTCTTGGAATGGAAATCAAGCAAAGCAAGGATGAAATTTTCATTTGTCAAAAGAAATACGCAAAAGAAATTTTGAAAAAGTTTAAGCTTGAAGAATGCAGAGAAATGAGCACTCCAATGAATTCAAAAGAGAAGCTTTGCAAAGAAGATGGAACTGAAAAGATTGATCAAGCATATTTTAGAAGTCTGATCGGTTGTTTGATGTATCTGACAGCCACACGGCCTGATATTTTGAATGCGGTAAGCATTTTGTCTCGGTTTATGCATTGTGCAAGTGAATGTCATCTCAAGGCAGCAAAGAGGGTGCTTAGATATGTGAAAGGAACATGTGATTTTGGCATTAAATTTACCAAAAGCAATGAATTCAAGCTGATTGGTTTTTCAGACAGCGATTGGGGAGGTTCTGCTGATGATATGAAAAGCACTTCTGGCTATTGTTTCACACTTGGTTCTAGTGTTTTCTCTTGGAGTTCGAAAAAACAAGAAACAGTGGCTCAATCAACTGCTGAAGCGGAGTTTATTGCTGCAACGGCTGCTGTTAATCAAGCTATGTGGCTGAGAAAGATATTGAGTGACCTTCATCTTGACCACAAAGAAGCCACTAAGATCTTTGTTGATAATCAAGCTGCAATAGCTATTTCTCATAATCCAGTTTTTCACGGAAAAACCAAGCATTTCAACATCAAATTGTTTTTCTTGAGGGAAGTGCAGAAGGATGGTGTCATTACTCTTGTTTATTGCAAAACAGAAGAGCAAAATGCTGATATCTTAACAAAGCCCTTATCAACTATCAAGTTTGAAGATATGAGATCAAAGCTTGGTGTTTGCAGCTCCTAAAGCAAGGAGGAGTGTTAAATATCATGCTTAGGACTGCTGATGTGAAGACTTTTATGTATTATGTGTTGGTTATTTTATTTTAGTAACTGCTGTTCAGTTTTCTTTAATTAATTGTGTTGTATTGTGGTAGTTATTGGAAGTTTTTAGTATTGTCAAACTATGTAAAACATAGTGTGTGTTCTGTTTGTTTTTTATTAGACAAATTATATGAATGAAAGCAGTTATTTCTCCTTTGTCTTGTTCATTTCCTGCAACTGTTTTCTTGTTCAATTAACAACAACTTTTGTACATTTAAGAGAGATCAAATTACACTTACTTGAGTACTGTCGTCGTTGATAAATTTATCAATTTGGGTGCATTTACTATAAAATTCATGGAGTTCAAGTTTATATCTATGCAATTAGTATGTAAACTGAATTACGATGTGAAAGAAGTATGATTTTTCATTATTAATCATTAAGAAATGAGTTTCTTCGTAATTCTATTGTTTTACTAATTGTCTGTTCGTGATGGCAGATAAAAGTGACAGTCCAAGGTTTTATTACTGAGGCCAGATGGTTGAACGAAAACTATATACCAACTATAGAAGAGTACATGCACAAATCAACAAAATCATGTTGTTACACTTTGTTGATATTAACTTCTTACATTGGAATGGGAGAAAATACCACGGAGAACATCTTCAATTGGGTAACAAATGACCCAAAAATTCTTAGTGCCGCAGCCAATCTTTGCAGGTTAATGGATGAAATTGTCTCCAGCGAGGTATTTAAATTATATCCAAAAAAATTATCTGTAATACACTCATTCCGTTTCATAATACATGTCGCTTTTGAAGAATAATTTTGTTTCATTTTACTTGTCACTTTCAAGTTTCAATGCAAGAATAATTATTAGGTTATCAATAATACCCTTACTTATTTATTGTAGAGAGATAAATAATATAATGAAATAATAAATGGGTAAGGTTATTATAGAAAAAACATAGAATTTCATCAAAAATAACAAAATTTATTTGATTTATTGGTATGTGTAACTTCCTCAAAAGCGACAAGTATTGTGAAACGGAGGGAGTATTATTTATTTTATGGGAAATGCTAGCATGTATCCAGATACTAAGGATTCCATTAGTAGAAATTATGTTTGAAATTTTATATTAATTTCAATAAAAGTAGAACATTTTTTTTTTGGAGAATGCTAATAAATGCCCTTGAGACTATGTTAATAAATACTTTCTATTAATTTTAATAGAAACTTTAACGTGTATCCTTAGAATACATGTTATCATGACTCTTATTTTATTTCACACGATATAAATACTCACATTAATTTTTTTTTGGTGTACAGTTCGAGCAAAAAAGAAAACATGTTTGCTCATTGTTGGATTGCTACATGAAGGAGTATGATATGTCTAGGGAAGCTGCTATTCAAGAATGCCAGAAGGGAATTGCAACTGCTTGGAAAGATATAAATGAGGAATGTCTTAGGCCAACCAAAGTTCCAATGCCTTTTCTTATTCGTGCTCTCAATTTTTCACGTTTTATTGATGTGATTTACAAAGATAACGACAATTACACCCATTCTCAAGGACTTATGAAGGTGTATATCAAAGAAGTCCTTGTTGATCGTGTTCCAATTTAAGCATCCAGTTTTAGTGAATGTTTAGATTCACGGCGAATTTGTCAAAATCTATGACACACCATGATCGTATGAAGTTCACCGTTAATACAAACATACATTTAGTCTTATGTGCTTTATATTTCTAGTTTGAATAAAAGAAAACATGTAATGTGTGCATGCCGTGTGTTGGATGCACACATTAATTTTATTATTATATTGTGTTTAATTTATATTGTGATGCATAAAGTTTGGTTTTGTTTTGCACTCTTTTTTCCTTCATCAGTTTCCATGTTTTACTGATAAAGTTTTTAATGAGGCAAAAGTTGTAGGTCATTTAAATTGTATCATTGGGTTTTGGCCTAGTCCCCCCAAGTCTCTTTATCTTATTTAATTTATAATAATGATTTGGGTGATGCCAATGATAGGTGTTGGTTGTGAGGGTGTCAATATAGTTTAAATGTCATCTCCACCATATGATGTCTTTGCTTTCTAATTTTGTATTTTGCTTAAATAAAAATAAGGAAATGCTAACTGGTGCCCTCGAACACGGGTTCAGGATACTAAAAAAGGAAATTACATAATAGTTACTGTATTGAAATTTGTATAATTAATCCATAAAAAGTCAAAAAAAAGTTTCATTTTTTATTAATAACTTCCTTTTTTTGACACCATTAACTAGTGTCCCAGGATACCAATTACCATGACCCTCCAATAAATAAATAAATAAATTTTTATCAACTTTTATTTGAAGTGTTTTTCAAGCGAATGCTTTGTATTTGCATGCATTCAAATCATTATGGCTGAAATCATCTAACCTAAGTAAACTTGGTAAGACTTATAAAGCTTGGAAAGTGTTGCTGGAAATCATTCTAGGGGATAAAATTATTAGAAAGTCCTAAAGTAGTAACTTGCTTAAAAAATATAAATAAAAAAGAAGCCATCAACAAAGGGAAACACCGATATATTTGTATCTCCTAAGACAGTTTTTCCAATTGCCATGCTTTAAATGCTTGCTCTTTTTCTTCCGATCTCGTGTTCATGTCCGCTGACAGTTTTGGAATGAAAGAAAAAGTGATTAAACACCAAGTAATGAAATTTTGATCTAGTATTGTGTGAATTAAAAAAAAATGTGAGTCTAATATAAAATATAACACACAATAGTAGTGTTTATATTGTGGTATTTGTTAGGTTTGTAGATTGTAATTGACCCTTTTTTGTTAAAACAAGTGTTGTAGCAAGATATTGAACAATATGCCTTGACATGTTGGTCCGACATCGTAGCGTGCCTTGTTTCACTTTCTTGAAAGATTTGTTTTAAACAATACATCATTGAAGATTTTATTGAAGATACGATTCACGTGTTGTCCAAGAAGCTTGTGATTTTAGAAGCTGCAAAGGTCGGTTTATGCATTACTGTGTTTCCTAAATTGCAGAGGTCGGTTTAGACATTTAAGGAAAATAATATATTTGCTCCTAGAAAATTGTGAAGATCATATATTCGTTTTGGTCTGCTGATTGTTTATCCTGTGAAAAAGCGCAAGCTGTTGAATGGCTATATAAAGGAAGACTCTAAACCTAAGAAAAAAACTAAAGACGTAATTGTCAAATTGTCTATTACGGTTTTAGTCTGTCCATTGTTATTTGTGAACCACTCTTGTATCACCTTGATGCTAGAGTTGGACTTGTAGTTGTGAATCACTCGTAAGCTTTTAAGCAATAGTGGATTCTGTTTCTCGGAGTGTGTCTCCATCTTAATTGTATTTGTTGTTATCACTAGCGAGTGATAAAGGGGAAGTGAGAGGGGTCTTGTATCTAACAGTGTCTTAGATAGAAATATCATGGGTAGTGATTAGGTGCGAAGCTATCAAAACAGAGGTTGTTTATGCAAGCTTCAAAGCAATACTATTATTGTGAATTTCCATCATGGCTTGGTAGCCCCCAGATGTAGGTGACGTTGTGTAACAACCCGCTTTTCGTTATAATTATTTTTATTATTTATTATATGTGTTATGTATGATTGACTGTCCTTGTGTGTAGGCATGGCAATGGGGCAAGGTGGGGACGGGTTTTGTCTCACCCATCCCCATCCCATACTCCCATATACTTACCCGTTACCCTACCCATACCCAACGGGGATGAGAAATTGAATCCCATCCCCGTCCCCAATGGGTTCGGGTATTCCCACCCCGTCCCCGCCCCTGCAGTGAATCAATTTTTTTAGTAAAAAAAAAGAGTTTTTTTAAGCTTCAATGGTAATGTTGTAATGCATTATCCAACAATTTGCGCTCATTTTAACATTTCCTTACCGAATAATTCGGTTGATCCTTTAACTATCAATGAAAGTTCTTATATCATGACAATTATCAAATCAAATAACATGGCATATCATTATCAACCTCCTCAACAAAAACAAAAATAAGACTCTACATTCGGGTAGGGTTTGGGTATGGGTCGGGGTGGATACATATATCCCCGTTACCCGTCCCGTACCTGTATTTTGAAATCGGGGAAAACCCAAACCCATACCCAAACCCAGTCAAATCGGGAAAAACCCGTCAAATCGGGTTTCGTTTGGGCGGGTACCCATGGATACAGGTTTTGTTGCCATGTCTACTTGTGTGTATTTTAATTGAGATTAGTGGATTTTTGGCATTAAAGGGTATTTTAGTCATTTTCAAACTAAAGGGTATTTTGGTCATTTGGCGAGTAAGGGTAAATTAGTCATTTTTATTAAGAAGGTTATTTTTGACATTTTAGTGAGAATGATTATTTTTACTAAGTTACTAGTGAGTTGGGGTAATTATTTAGAGATGGTAATTATTTGGTGGTTTAACGGTTTAAGTGAGTAGTAACTTTTGAGAATTAGTTAGTAAGCATGTTAAGCCCATTATGACAATTAGAAACCTAACTCAATTAAGAGGTGACTATATAAAACATAACTCTTAAGAGAAAACTAGGTTTTCCCATTCATTTTTCATTTCACAAACTTAAAAGGTAGAGAGAGAAAGTACGACCACAAACTTGGAGAGGAGGAAAGAAGAGGAAGCTAGAGAAAGCTAGGGGTTTGAGAGCTACAGAGTTGATTGAAGCTTAAGTTAGTGGATTCAAGACCTAGAGGTAAGGGGGATAGATTCCTTTCATCAATTAATCTGTAATTTCAAATTTTGATTATGGGTCATAAGTGGTTATTTGTGAATTATGATTTTGATGAATTTATATGTTGATTTTCGTGCTCATGTTTGTGTGATAAATTTGGGTATGTGAAATTGGTCATACACTTGTTGATTATGCTTAAACTTCATGGTGATTATGTGTTAAAATTATTATGTTGAACTTTTAGCTTAAAATGATGAAAAGTGATGTTGTTGTTGATTGTTGATGAACTCATGAATTTATGTTTAGATTGTTGTTGAGTTATGTTGTTGATGCTAATTCATCCTTTGGGTATTGCAAAATGTGTTGTTGTGATTAAGTTGTTGTTGTTTGATGATTTTTCGTGAAAATAGGTTGAAAATGAAAAATTTGATTTTAATGAATTTGAATGAATTGATGAAGTTGTTAGAATGAATTAGGATGATGGAAATTCTGTTTTTAATCACTTTGTTTTCGTGTGAAATGTTTATTTTTATTTTTTTATAAACACTCTAGATGTGTTCACCAAGTTTAAGGATGAGCAAGGAAACTTTAATAAAAGACTAATCACCAACGTCGAAGGTATGTTAAGCTTGTATGAAGCCTCACATATGATTATTCATGGAGAGGAAATTTTGGAAGAGGCATTGGCTTTTACGTCCACTCACCTTGAGTTCACTGCCACCCAAATGAGCACTTTCTTGCAGCACAAGTCAAATATGCTTTAAGACAGGCTCTCCACAAGAACTTGCCTAGGCTAGACGCACGACGGTACATTTCTATGTACGAGCAAAATCCTTCACGTAATGAACTTTGGCAAAATTAGATTTTAATTTGCTCCAAAAGCCTACATCAAAAAGAGTTTGGAAACATTTGCAAGTAAGTATTTCAATCACTCTCCGATTCTTTGTAACGAAACAAAATTAAAGTGATCATAAGGAGAAATCCATGTAATAACTATTATATATTGATTTTGATCTTTAAAGATGGTGGAAGGAGTTGAACTTCTCTAGTAAACTGCCTTATGCACGTCATAGGATTGTCGAATGTTGCTTCTGGATTTTGACCGCATATTTTGAGCCCCAATATTCTCTAGCAAGGAAAATAACGACGAAAGTAATCACGATGTTATCAATCATTGATGATACATATGATGCATATGGAACCATTGATGAACTAGAACTTTTTACCAAGGCTATTCAAAGGTTTGTCATCATAGATTTATTGAACAAAAGAACTGGAACATATATTTGCATTTCTCCCTTGCTTACAAAAAATATTTTTCCATCTATTTAAAAAAGTTAAAAGTGATTTTCAGGTGGGATATTAGCATCTTGGATGATCTTCCAGACTACATGAAATTAATTTATGGAACACTCTGGAATGTTTTCGAAGAAGTAGAGCAGGAAATGAGGAAAGAAGGAAAGCTCTATACCATGAAGTACTTTATAAAAGAAGTATGAGTTTGTGCCTTCTCTAATTAATTATTAGCAACAAAACATGAGCCATAACTTATTTATACATATAATAAGTATTATTTTTTTTATGAGCAAAGATAAATTATATTAATAAGGACAATCAATAAATCAGAACGAAATTGATTGATTTTAATGATACTATTTTGTTTTGACCCTCATATTCTTAGGGGAATAGTGAGTTGATAATCCAAAGCTGATTTTTATTTTTTGACAGTCAATCCAGAGCTGATTAAGAATTGTTTAAACATTCAAACTCAGATTATTTCGAAGGATTCATCCTTAAATCAATTTCATTAATCACTTGATACCACTCAATTAATTAAATGTTACTCCTTTCTTTAACTACAAGTGGTTGACTTTGCTTAACTCAACTATTAAACAAATATTGAATGTCTTGTTTGTTTAAGAGTGAAAGTTCATGCAAAAGTTTAGGAGCAAAGTCTCAGCCTTTATTTGGTTGGAAACAAATTTAATTTTAATTCTGTGAAGGAATTTTTGTTACACAATTTATTTTGTGAAGTTAAGAAACTTAACATTACTATTTTTGTACATTTAAGAGAGATCAAATTACACTTGAATAATACTCGAGTACTGTCGTCGTTGATAAATTAATCAATTTGTGTACATTTACTATAAAATTTATGGGTCATGCTAACTTGTGTCCTAAAGGCACATCTTAGACAATCTAAAAGTGGAAACATTCTCTTAAATTTTATGCATTCAATTAACTAAAAATAAAAAAAATAAATTCAAATGCAATAATTGCAACAAATTATTTTTATTTTTGACTCCTTAGCTTGTGCCTTAAGGGCACAAGTTAGCGACAAATTATATCAATGCAATTTGTACTTATTATGACTATGTAAACTGAATTACGTTGTGAAAGAAGTATGATTTTTCATTATTAATCATTAAGAAATGAGTTTCTTAGTAATTCTATTGTTTTAATTGCCTGTTCGGGATGGCAGATAAAAGTGGCAGTCCAAGGTTTTATTACTGAGGCCAGATGGCTGAACGAAAACTATGTTCTTACACTTTGTTGATATTAACTTCTTACATTTGTGTAAATACTTTTGGGTGGTTTGCCCCTTGCACACCTTGTGCGGGGTGAATCATTTTTGTTAATATATATCATTTTAGTTTGTTCAAAAAAAAAAAAAAAAAAACTTCTTACATTGGAATGGGAGAAAATACCACGGAAAACATCTTCAATTGGGTAACAAATGACCCAAAAATTCTTAGTGCCGCAGCCAATCTTTGCAGGTTAATGGATGAAATTGTCTCCAATGAGGTATTTAAAATATATCAAAAAACTTATCTATATATACTCTCTCCGTCTTATAATAACTGAGTCATTTGCAACACAAAAAAAAATGTTTCAAAATAATTGAGTCATTTCACATTTTAATACAATATTAATTACTTTTTTTCCTTTATAACTCTAATTAATATTACTCTCATCACTCTCAATTCAATATATTTCACTTTGCATTTATGATAGTGGAGAATGCACCAATACTTAACAAGAGCACTTAAAACCATTTTTCTCTCTATTTCATTTATACATTTTTCTTAATATGGGTGAAATGAGCAAATGAATCAGTTAAACTGGGACGGATGGAGTACTATTTATTTTATAGGAAATATGAGTAACATGTATTCAAATGTTAAGGATTCCATCAGTAAAATTATATATTGGAAATTGTACAAATTAATTTCAACAAAAGTAAGACATTAATTTTTCTTAATACAGTAAATACTATCTATTAATGGAAACTTTAATGTGTGCCATAAAGACACATGTTATATGACCCTTATTTTATTTCACACGATATAAATTAAATACATGCGTTAATGATTTCTTGGTGTACAGTTCGAGCATAAAAGAGAACATGTTTGCTCATTGTTGCATTGCTACATGAAGCAGTATGATATGTCTAGGGAAGCTGCTATTCAAGAATGCCAGAAGGGAATTGCAATTGCTTGGAAAGATATAAATGAGGAATGTCTTAGGCCAACCAAAGTTCCAATGCCTTTTCTTATTCGTGCTCTCAATTTTTCACGTTTTATTGATGTGATTTACAAAGATAAAGACAATTACACACATTCTGAAGGACTTATGAAGGCGTATATCAAAGAAGTCCTTGTTGATCGTGTTCCAATCTAA
Protein sequence

>MS.gene035927.t1

MSTVAYSNPDAKQRNLAEFHPNVWGEYFIQYASESMELDQNIAAQIVKLKDEVRNMLVSKTEMPLAKVTLIDSICRLGVSYHFENEIDELLQHFHKSYVENGEIVLEDSLYSLAVLFRVLRQQGFYVSPNVFTKFKDEEGNFNKELITDVEGMLSLYEASHMIIHGEEILEEALAFTSTHLESIATQTSHFLAAQVKYALRQALHKNLPRLEARRYISIYEQNPSHDEILLTLAKLDFNLLQSLHQKEFGNICKWWKELDFSSKLPYARHRIVECCFWILTAYFEPQYSLARKLATKVIAMLSIIDDTYDAYGTIDELELFTKAIQRWDISILDDLPDYMKLIYETLWNIFEEIEQEMRKEGKLYTMKYFIKEIKVTVQGFITEARWLNENYIPTIEEYMHKSTKSCCYTLLILTSYIGMGENTTENIFNWVTNDPKILSAAANLCRLMDEIVSSEFEQKRKHVCSLLDCYMKEYDMSREAAIQECQKGIATAWKDINEECLRPTKVPMPFLIRALNFSRFIDVIYKDNDNYTHSQGLMKVYIKEVLVDHVFTKFKDEQGNFNKRLITNVEARKITTKVITMLSIIDDTYDAYGTIDELELFTKAIQRWDISILDDLPDYMKLIYGTLWNVFEEVEQEMRKEGKLYTMKYFIKEFEHKREHVCSLLHCYMKQYDMSREAAIQECQKGIAIAWKDINEECLRPTKVPMPFLIRALNFSRFIDVIYKDKDNYTHSEGLMKAYIKEVLVDRVPI