AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene036826

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036826.t1 MTR_8g014160 47.537 406 150 8 12 370 36 425 1.01e-105 317
MS.gene036826.t1 MTR_8g014260 37.895 475 166 13 1 370 1 451 2.92e-78 248
MS.gene036826.t1 MTR_4g113810 62.500 168 19 1 116 239 1 168 1.41e-63 200
MS.gene036826.t1 MTR_8g077460 35.714 420 175 13 1 370 1 375 2.28e-63 207
MS.gene036826.t1 MTR_8g445780 35.294 425 140 11 1 325 1 390 2.29e-60 199
MS.gene036826.t1 MTR_2g083840 33.110 447 174 13 7 351 11 434 6.25e-54 184
MS.gene036826.t1 MTR_8g467800 32.009 453 169 13 6 345 3 429 1.92e-53 183
MS.gene036826.t1 MTR_8g468840 29.211 469 207 14 1 367 2 447 3.61e-47 166
MS.gene036826.t1 MTR_4g024710 38.246 285 115 4 131 361 60 337 8.82e-46 160
MS.gene036826.t1 MTR_4g024745 37.457 291 119 6 118 353 1 283 5.61e-45 156
MS.gene036826.t1 MTR_8g468760 33.094 417 189 14 13 356 5 404 1.28e-44 159
MS.gene036826.t1 MTR_8g014230 82.759 87 15 0 284 370 18 104 7.12e-44 147
MS.gene036826.t1 MTR_8g468810 30.719 459 187 14 1 349 1 438 1.84e-42 154
MS.gene036826.t1 MTR_5g092960 36.646 322 131 11 17 284 19 321 6.20e-42 152
MS.gene036826.t1 MTR_5g092960 44.872 78 40 2 285 361 377 452 2.69e-12 68.2
MS.gene036826.t1 MTR_5g092960 36.646 322 131 11 17 284 19 321 1.08e-41 150
MS.gene036826.t1 MTR_8g062270 35.873 315 137 9 17 284 16 312 1.88e-41 151
MS.gene036826.t1 MTR_8g467800 33.033 333 143 10 6 284 3 309 1.28e-39 144
MS.gene036826.t1 MTR_5g021550 44.022 184 72 4 5 182 4 162 1.32e-39 138
MS.gene036826.t1 MTR_8g469020 29.936 471 197 17 12 370 2 451 3.97e-39 145
MS.gene036826.t1 MTR_8g070950 30.769 468 192 19 1 370 1 434 3.58e-37 139
MS.gene036826.t1 MTR_8g062010 29.978 457 197 19 8 370 3 430 8.12e-37 138
MS.gene036826.t1 MTR_8g058965 30.105 475 196 16 12 370 2 456 1.59e-35 135
MS.gene036826.t1 MTR_8g468810 32.941 340 147 12 1 284 1 315 1.65e-35 132
MS.gene036826.t1 MTR_8g468810 32.941 340 147 12 1 284 1 315 2.66e-35 132
MS.gene036826.t1 MTR_8g468810 32.941 340 147 12 1 284 1 315 3.08e-35 132
MS.gene036826.t1 MTR_8g014210 39.744 234 78 6 194 370 4 231 4.83e-35 129
MS.gene036826.t1 MTR_8g468810 32.941 340 147 12 1 284 1 315 6.09e-35 132
MS.gene036826.t1 MTR_8g014290 46.043 139 64 2 15 153 8 135 2.33e-34 124
MS.gene036826.t1 MTR_8g062310 28.761 452 188 17 11 370 19 428 3.45e-34 131
MS.gene036826.t1 MTR_4g024720 46.897 145 58 4 1 144 1 127 9.62e-33 122
MS.gene036826.t1 MTR_8g058970 31.897 348 152 13 3 284 2 330 1.16e-32 127
MS.gene036826.t1 MTR_8g058970 43.011 93 47 4 284 370 385 477 2.12e-11 65.5
MS.gene036826.t1 MTR_4g032730 30.591 389 183 20 1 339 1 352 3.14e-29 118
MS.gene036826.t1 MTR_4g032730 53.333 60 27 1 286 344 382 441 3.32e-13 70.9
MS.gene036826.t1 MTR_4g050250 27.088 443 168 14 3 317 4 419 1.07e-27 113
MS.gene036826.t1 MTR_7g032380 28.255 361 208 8 17 356 11 341 2.05e-25 105
MS.gene036826.t1 MTR_3g108150 27.543 403 202 13 16 363 12 379 3.41e-24 102
MS.gene036826.t1 MTR_0543s0010 26.952 397 231 15 8 369 77 449 4.72e-24 103
MS.gene036826.t1 MTR_1g041245 27.708 397 194 12 17 356 11 371 3.80e-23 99.8
MS.gene036826.t1 MTR_0492s0020 26.562 384 224 14 8 357 77 436 4.46e-23 100
MS.gene036826.t1 MTR_8g014220 36.612 183 88 6 1 178 1 160 5.40e-22 91.7
MS.gene036826.t1 MTR_8g467790 27.614 373 172 17 17 317 148 494 6.20e-22 97.4
MS.gene036826.t1 MTR_2g086020 31.161 353 179 16 43 370 2 315 8.38e-22 95.1
MS.gene036826.t1 MTR_4g024770 60.000 65 26 0 1 65 8 72 8.85e-22 88.6
MS.gene036826.t1 MTR_7g098570 24.552 391 242 10 7 361 10 383 2.56e-21 95.5
MS.gene036826.t1 MTR_7g033990 26.185 401 208 12 17 364 11 376 1.92e-20 92.4
MS.gene036826.t1 MTR_7g098510 27.431 401 247 13 2 370 3 391 4.28e-20 91.3
MS.gene036826.t1 MTR_6g022500 27.968 379 181 13 17 339 101 443 4.53e-20 91.7
MS.gene036826.t1 MTR_5g073370 26.209 393 183 14 14 344 2 349 9.59e-20 90.1
MS.gene036826.t1 MTR_7g098480 26.566 399 222 16 2 356 3 374 1.85e-19 89.7
MS.gene036826.t1 MTR_3g105060 26.111 360 212 10 17 345 14 350 3.87e-19 88.2
MS.gene036826.t1 MTR_8g014180 62.069 58 22 0 1 58 1 58 8.70e-19 79.7
MS.gene036826.t1 MTR_7g056420 26.098 387 206 13 16 361 8 355 9.16e-19 88.6
MS.gene036826.t1 MTR_7g056430 26.098 387 206 13 16 361 8 355 1.01e-18 88.6
MS.gene036826.t1 MTR_3g108560 24.518 363 218 12 14 341 17 358 1.53e-18 86.7
MS.gene036826.t1 MTR_7g026500 26.582 395 196 14 3 337 2 362 1.54e-18 86.7
MS.gene036826.t1 MTR_8g062080 31.624 234 83 11 5 187 7 214 1.80e-18 86.7
MS.gene036826.t1 MTR_8g062080 45.161 93 48 3 281 370 353 445 1.74e-13 71.6
MS.gene036826.t1 MTR_1089s0010 27.143 350 216 12 17 340 8 344 3.91e-18 85.5
MS.gene036826.t1 MTR_3g467400 28.238 386 195 15 17 356 3 352 1.78e-17 83.2
MS.gene036826.t1 MTR_3g023630 26.404 356 217 11 17 342 8 348 1.83e-17 83.2
MS.gene036826.t1 MTR_8g085430 26.685 371 173 11 17 356 11 313 3.66e-17 82.0
MS.gene036826.t1 MTR_7g033915 25.326 383 216 12 17 364 11 358 1.03e-16 80.9
MS.gene036826.t1 MTR_3g113782 48.438 64 33 0 1 64 1 64 7.80e-15 69.7
MS.gene036826.t1 MTR_7g056433 24.213 413 229 14 12 370 7 389 1.00e-14 75.1
MS.gene036826.t1 MTR_0009s0080 25.255 392 203 16 6 339 8 367 1.06e-14 75.5
MS.gene036826.t1 MTR_5g058100 26.234 385 187 17 17 343 9 354 1.91e-14 74.3
MS.gene036826.t1 MTR_7g056447 63.158 57 20 1 16 71 8 64 1.94e-14 73.6
MS.gene036826.t1 MTR_6g040270 26.705 352 213 15 19 340 10 346 3.04e-14 73.9
MS.gene036826.t1 MTR_3g082890 50.000 66 33 0 1 66 1 66 1.63e-13 72.0
MS.gene036826.t1 MTR_3g082890 50.000 66 33 0 1 66 81 146 2.65e-13 71.2
MS.gene036826.t1 MTR_3g107100 27.632 380 204 15 11 343 5 360 2.80e-13 70.9
MS.gene036826.t1 MTR_7g098590 50.000 70 34 1 3 71 4 73 2.97e-13 66.6
MS.gene036826.t1 MTR_8g062300 33.708 178 79 8 1 163 1 154 7.93e-13 69.7
MS.gene036826.t1 MTR_8g062300 41.489 94 50 4 281 370 385 477 2.55e-11 65.1
MS.gene036826.t1 MTR_1g085120 24.265 408 210 15 16 361 91 461 8.91e-13 69.7
MS.gene036826.t1 MTR_1g038760 50.000 60 30 0 8 67 6 65 1.34e-12 68.6
MS.gene036826.t1 MTR_1g038760 50.000 60 30 0 8 67 6 65 1.48e-12 68.6
MS.gene036826.t1 MTR_1g085120 24.324 407 211 15 16 361 166 536 1.54e-12 68.9
MS.gene036826.t1 MTR_5g083890 64.583 48 17 0 16 63 5 52 1.86e-12 68.9
MS.gene036826.t1 MTR_3g114200 53.030 66 27 2 4 65 2 67 2.59e-12 64.7
MS.gene036826.t1 MTR_2g007360 29.000 200 111 6 8 192 3 186 3.14e-12 67.8
MS.gene036826.t1 MTR_2g082860 43.902 82 40 3 6 82 17 97 3.76e-12 63.2
MS.gene036826.t1 MTR_7g034090 26.393 341 180 13 16 341 3 287 4.50e-12 67.4
MS.gene036826.t1 MTR_6g011460 24.868 378 191 13 17 339 46 385 5.21e-12 67.0
MS.gene036826.t1 MTR_3g040700 23.362 351 206 10 15 329 41 364 5.54e-12 67.0
MS.gene036826.t1 MTR_3g099830 54.902 51 23 0 17 67 12 62 9.53e-12 66.2
MS.gene036826.t1 MTR_7g115270 36.029 136 60 4 16 151 11 119 9.73e-12 66.2
MS.gene036826.t1 MTR_5g060570 54.902 51 23 0 16 66 16 66 1.58e-11 64.3
MS.gene036826.t1 MTR_4g080787 25.000 316 175 13 16 282 18 320 1.63e-11 65.9
MS.gene036826.t1 MTR_1g095750 47.143 70 36 1 5 73 34 103 1.73e-11 62.0
MS.gene036826.t1 MTR_3g108560 27.027 185 110 5 14 182 17 192 1.73e-11 65.1
MS.gene036826.t1 MTR_7g033940 55.556 54 23 1 19 71 96 149 1.79e-11 63.5
MS.gene036826.t1 MTR_7g033945 55.357 56 24 1 17 71 11 66 1.88e-11 62.8
MS.gene036826.t1 MTR_7g110960 56.522 46 20 0 6 51 5 50 1.99e-11 59.3
MS.gene036826.t1 MTR_1g053025 24.725 364 229 13 17 339 2 361 2.41e-11 65.1
MS.gene036826.t1 MTR_1g115245 50.000 68 33 1 2 69 12 78 2.81e-11 64.3
MS.gene036826.t1 MTR_7g007340 25.895 363 205 13 6 339 2 329 3.01e-11 64.7
MS.gene036826.t1 MTR_2g072720 27.211 147 86 2 5 151 27 152 3.99e-11 64.7
MS.gene036826.t1 MTR_8g066650 47.826 69 32 1 7 71 70 138 4.76e-11 64.3
MS.gene036826.t1 MTR_7g081290 51.667 60 29 0 6 65 7 66 5.21e-11 62.8
MS.gene036826.t1 MTR_7g007370 29.032 186 105 7 1 185 1 160 5.46e-11 63.5
MS.gene036826.t1 MTR_4g080773 32.432 111 68 2 16 126 18 121 7.47e-11 63.9
MS.gene036826.t1 MTR_4g080773 32.432 111 68 2 16 126 18 121 7.74e-11 63.9
MS.gene036826.t1 MTR_2g008320 49.153 59 30 0 9 67 44 102 8.35e-11 63.5
MS.gene036826.t1 MTR_7g075820 60.417 48 19 0 16 63 5 52 8.76e-11 63.2
MS.gene036826.t1 MTR_4g045680 44.776 67 33 1 9 71 8 74 8.96e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036826.t1 AT1G32375 24.541 436 232 18 17 370 2 422 5.65e-15 76.3
MS.gene036826.t1 AT1G32375 24.541 436 232 18 17 370 2 422 5.65e-15 76.3
MS.gene036826.t1 AT5G38386 25.575 348 205 16 17 350 2 309 2.68e-14 73.9
MS.gene036826.t1 AT5G38386 25.575 348 205 16 17 350 2 309 2.68e-14 73.9
MS.gene036826.t1 AT3G59200 30.455 220 124 8 17 234 7 199 7.01e-14 72.8
MS.gene036826.t1 AT3G59200 30.455 220 124 8 17 234 7 199 3.12e-13 71.2
MS.gene036826.t1 AT3G60040 25.263 285 150 11 16 249 12 284 7.85e-13 70.1
MS.gene036826.t1 AT3G60040 25.263 285 150 11 16 249 231 503 1.02e-12 70.1
MS.gene036826.t1 AT3G60040 25.263 285 150 11 16 249 232 504 1.06e-12 70.1
MS.gene036826.t1 AT3G60040 25.263 285 150 11 16 249 229 501 1.30e-12 69.7
MS.gene036826.t1 AT3G60040 25.263 285 150 11 16 249 231 503 1.33e-12 69.7
MS.gene036826.t1 AT3G59000 23.514 387 201 13 17 333 2 363 1.18e-11 66.2
MS.gene036826.t1 AT1G49610 29.524 210 101 9 10 209 19 191 1.91e-11 65.1
MS.gene036826.t1 AT3G58950 23.229 353 210 11 17 350 2 312 3.27e-11 64.7
MS.gene036826.t1 AT1G16930 25.776 322 166 12 10 282 8 305 3.56e-11 64.7
MS.gene036826.t1 AT1G16930 25.776 322 166 12 10 282 8 305 3.56e-11 64.7
MS.gene036826.t1 AT1G16930 25.776 322 166 12 10 282 8 305 3.56e-11 64.7
MS.gene036826.t1 AT3G58900 24.163 418 203 15 17 358 2 381 4.48e-11 64.3
MS.gene036826.t1 AT3G58940 29.126 206 112 8 17 213 2 182 5.09e-11 64.7
MS.gene036826.t1 AT3G58940 29.126 206 112 8 17 213 2 182 5.09e-11 64.7
MS.gene036826.t1 AT3G59000 32.886 149 74 5 17 162 2 127 6.36e-11 63.5

Find 75 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGACGCATCTTCCGAATT+TGG 0.256496 8.1:-12720941 None:intergenic
TAACATCACCAAATCTGATT+TGG 0.269584 8.1:-12721527 None:intergenic
GCTTGTTTCAACAGAGCTTT+TGG 0.290992 8.1:-12720715 None:intergenic
TTCCTCGAGTGATCATTCTT+TGG 0.294564 8.1:+12721593 MS.gene036826:CDS
AACCTTACCCAAATCAGATT+TGG 0.328338 8.1:+12721519 MS.gene036826:CDS
CAGGAAGAGTCGCCTTTATC+AGG 0.330231 8.1:+12722251 MS.gene036826:intron
AAAACACGCCGTTTACAAAA+AGG 0.351262 8.1:-12720899 None:intergenic
TCAATAAACCCTACCAAATT+CGG 0.357859 8.1:+12720928 MS.gene036826:CDS
CTTTAAACTGAATAAAAGTT+AGG 0.359295 8.1:-12722316 None:intergenic
GATTATTGATGATATTTCAT+TGG 0.361849 8.1:+12722412 MS.gene036826:CDS
GAAACTCCCTGGTATGAAAA+TGG 0.366711 8.1:+12721317 MS.gene036826:CDS
CTCGCAGATGGTGCAACGTT+TGG 0.370015 8.1:+12720745 MS.gene036826:CDS
TGGCGTAATCCTCATAATAA+AGG 0.376607 8.1:-12721395 None:intergenic
ACATGGGTCCGCTCCATCAT+TGG 0.380102 8.1:+12721014 MS.gene036826:CDS
AGGTGTGATATGATACAATT+AGG 0.381715 8.1:-12722296 None:intergenic
TCAACAAATAATAGCTCATC+TGG 0.390858 8.1:-12722353 None:intergenic
GAATACTTACCTTTATTATG+AGG 0.396465 8.1:+12721386 MS.gene036826:CDS
TTATCCCAGAGAGAGCATTA+AGG 0.398455 8.1:-12721638 None:intergenic
AACATCACCAAATCTGATTT+GGG 0.403607 8.1:-12721526 None:intergenic
AAGCTCCTTAATGCTCTCTC+TGG 0.404539 8.1:+12721633 MS.gene036826:CDS
CGTAGGTGGGAAACAAATAT+TGG 0.406304 8.1:-12721415 None:intergenic
GAAATGAATGCATTCCTTGT+TGG 0.409529 8.1:+12721342 MS.gene036826:CDS
ATTCAGTTTAAAGGATATCA+AGG 0.428602 8.1:+12722326 MS.gene036826:CDS
CATGGGTCCGCTCCATCATT+GGG 0.432825 8.1:+12721015 MS.gene036826:CDS
CAGCTCAAGAACAAGAAGAT+AGG 0.434378 8.1:+12720640 MS.gene036826:CDS
CCGCAATATCGTCGAGTATG+AGG 0.439320 8.1:-12721058 None:intergenic
GTCAAATTTGTCAAATTCAA+AGG 0.439842 8.1:-12720802 None:intergenic
GAGCTTGAATAAACATTTGA+AGG 0.443022 8.1:-12721616 None:intergenic
GAATGTCGCACTTCTTACTT+AGG 0.444977 8.1:-12722436 None:intergenic
CATCTTGGTCCATGTCGTCT+TGG 0.463453 8.1:-12720839 None:intergenic
GGGATAAAACATCTATCGTT+GGG 0.485986 8.1:+12721654 MS.gene036826:CDS
ATGTGGCTCAGTATACAGTC+TGG 0.488231 8.1:-12720675 None:intergenic
ATCGTTGGTCCATGTCATCT+TGG 0.488259 8.1:-12720854 None:intergenic
TGTGTAGCCCATCCTGATAA+AGG 0.492199 8.1:-12722263 None:intergenic
CAACTCAGTCAAATATAAGT+TGG 0.492762 8.1:-12722496 None:intergenic
AGGTCAAGTTCCTTGAGGTC+AGG 0.495409 8.1:-12721038 None:intergenic
CTTGAGGTCAGGCCCAATGA+TGG 0.497145 8.1:-12721027 None:intergenic
TGGGATAAAACATCTATCGT+TGG 0.498569 8.1:+12721653 MS.gene036826:CDS
ATAAGTTATATAAAGTGCTA+AGG 0.498850 8.1:+12722462 MS.gene036826:CDS
CATCATTGGGCCTGACCTCA+AGG 0.499353 8.1:+12721028 MS.gene036826:CDS
CACCAAATCTGATTTGGGTA+AGG 0.502806 8.1:-12721521 None:intergenic
CGCATCTTCCGAATTTGGTA+GGG 0.504606 8.1:-12720936 None:intergenic
ACGCATCTTCCGAATTTGGT+AGG 0.508995 8.1:-12720937 None:intergenic
TGCAAACTCTTCAACGTAGG+TGG 0.512529 8.1:-12721429 None:intergenic
CTAGGATAGGGCAGCCAACA+AGG 0.529192 8.1:-12721356 None:intergenic
TTAGGAAAAGCTTAAATCGT+TGG 0.529258 8.1:-12720869 None:intergenic
TGGCAGAGTCGAACTCCAAA+CGG 0.540408 8.1:+12720610 MS.gene036826:CDS
CTGAAGAAGTTGAAACTCCC+TGG 0.545724 8.1:+12721306 MS.gene036826:intron
AGCTCCTTAATGCTCTCTCT+GGG 0.549116 8.1:+12721634 MS.gene036826:CDS
AAGAGTCGCCTTTATCAGGA+TGG 0.553310 8.1:+12722255 MS.gene036826:CDS
GCAGATGTTCAATAGATACA+TGG 0.554380 8.1:+12720997 MS.gene036826:CDS
TAAGTTATATAAAGTGCTAA+GGG 0.555537 8.1:+12722463 MS.gene036826:CDS
GCAAACTCTTCAACGTAGGT+GGG 0.556690 8.1:-12721428 None:intergenic
CGACATGGACCAAGATGACA+TGG 0.563661 8.1:+12720845 MS.gene036826:CDS
ATGATACAATTAGGAACACT+TGG 0.569360 8.1:-12722287 None:intergenic
TGGGTAAGGTTAAGATAGTG+AGG 0.571605 8.1:-12721507 None:intergenic
TTATATAAAGTGCTAAGGGA+TGG 0.571621 8.1:+12722467 MS.gene036826:CDS
CTCATGTACAAAATCTCTCG+TGG 0.589169 8.1:+12720962 MS.gene036826:CDS
ATTTGCAAACTCTTCAACGT+AGG 0.595550 8.1:-12721432 None:intergenic
TGACATAGACCAAGACGACA+TGG 0.596420 8.1:+12720830 MS.gene036826:CDS
AAAGAATGATCACTCGAGGA+AGG 0.599289 8.1:-12721591 None:intergenic
CCTCATACTCGACGATATTG+CGG 0.603384 8.1:+12721058 MS.gene036826:CDS
TTCATCAGAAAAGTCGAGAA+CGG 0.605182 8.1:-12720778 None:intergenic
GATGTTGATATCGCTTCTGT+TGG 0.605436 8.1:+12721456 MS.gene036826:CDS
CAAGCATTCTATCTCGCAGA+TGG 0.608644 8.1:+12720733 MS.gene036826:CDS
CAGATGTTCAATAGATACAT+GGG 0.617815 8.1:+12720998 MS.gene036826:CDS
AGAGTCGCCTTTATCAGGAT+GGG 0.619421 8.1:+12722256 MS.gene036826:CDS
TGTGGCTCAGTATACAGTCT+GGG 0.619641 8.1:-12720674 None:intergenic
AGGTTAAGATAGTGAGGACA+AGG 0.625769 8.1:-12721501 None:intergenic
GGCCAAAGAATGATCACTCG+AGG 0.634076 8.1:-12721595 None:intergenic
GTTGGGTTGCTCAACAACAA+AGG 0.654398 8.1:+12721671 MS.gene036826:CDS
GTATGAGGTCAAGTTCCTTG+AGG 0.672067 8.1:-12721043 None:intergenic
GGTCAGGCCCAATGATGGAG+CGG 0.674038 8.1:-12721022 None:intergenic
TTGGAAGGAAAGACATGATG+TGG 0.683179 8.1:-12720692 None:intergenic
TTAAGATAGTGAGGACAAGG+CGG 0.728130 8.1:-12721498 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTTCACATCATTAAG+TGG - chr8.1:12722130-12722149 None:intergenic 20.0%
!! CTTTAAACTGAATAAAAGTT+AGG - chr8.1:12722319-12722338 None:intergenic 20.0%
!! GTATAGTTTATTATCATAGT+TGG + chr8.1:12721693-12721712 MS.gene036826:intron 20.0%
!! TTTATCAGTCATTTACAATT+TGG - chr8.1:12721733-12721752 None:intergenic 20.0%
!!! ATAAGTTATATAAAGTGCTA+AGG + chr8.1:12722462-12722481 MS.gene036826:CDS 20.0%
!!! CTAACTTTTATTCAGTTTAA+AGG + chr8.1:12722317-12722336 MS.gene036826:CDS 20.0%
!!! GATTATTGATGATATTTCAT+TGG + chr8.1:12722412-12722431 MS.gene036826:CDS 20.0%
!!! GTTGAGTATATTTTGAAAAA+AGG + chr8.1:12722371-12722390 MS.gene036826:CDS 20.0%
!!! TAAGTTATATAAAGTGCTAA+GGG + chr8.1:12722463-12722482 MS.gene036826:CDS 20.0%
! AAGATGTAATCATGCATATA+TGG - chr8.1:12722203-12722222 None:intergenic 25.0%
! AATGAAATAGATTACACAAG+AGG + chr8.1:12722164-12722183 MS.gene036826:intron 25.0%
! ATATACACCACAATTTACTA+AGG + chr8.1:12721263-12721282 MS.gene036826:intron 25.0%
! ATTACATCTTATGTATGTTG+TGG + chr8.1:12722213-12722232 MS.gene036826:intron 25.0%
! ATTCAGTTTAAAGGATATCA+AGG + chr8.1:12722326-12722345 MS.gene036826:CDS 25.0%
! GAATACTTACCTTTATTATG+AGG + chr8.1:12721386-12721405 MS.gene036826:CDS 25.0%
! GTCAAATTTGTCAAATTCAA+AGG - chr8.1:12720805-12720824 None:intergenic 25.0%
! TGAGTTCCAAATGAATTAAA+TGG - chr8.1:12721981-12722000 None:intergenic 25.0%
!! ATTCTGCCATTTAATTCATT+TGG + chr8.1:12721972-12721991 MS.gene036826:intron 25.0%
!!! TATTCAGATTTTCTAGGATA+GGG - chr8.1:12721371-12721390 None:intergenic 25.0%
AACATCACCAAATCTGATTT+GGG - chr8.1:12721529-12721548 None:intergenic 30.0%
ACAATTAACATGTACAGTCT+TGG + chr8.1:12721231-12721250 MS.gene036826:intron 30.0%
AGGTGTGATATGATACAATT+AGG - chr8.1:12722299-12722318 None:intergenic 30.0%
ATATCACTAACAATAGTCCT+AGG + chr8.1:12721815-12721834 MS.gene036826:intron 30.0%
ATGATACAATTAGGAACACT+TGG - chr8.1:12722290-12722309 None:intergenic 30.0%
CAGATGTTCAATAGATACAT+GGG + chr8.1:12720998-12721017 MS.gene036826:CDS 30.0%
GAGCTTGAATAAACATTTGA+AGG - chr8.1:12721619-12721638 None:intergenic 30.0%
GCATATATGGTAATAAATCG+AGG - chr8.1:12722190-12722209 None:intergenic 30.0%
GGACTATTGTTAGTGATATT+AGG - chr8.1:12721814-12721833 None:intergenic 30.0%
TAACATCACCAAATCTGATT+TGG - chr8.1:12721530-12721549 None:intergenic 30.0%
TCAACAAATAATAGCTCATC+TGG - chr8.1:12722356-12722375 None:intergenic 30.0%
TCAATAAACCCTACCAAATT+CGG + chr8.1:12720928-12720947 MS.gene036826:CDS 30.0%
TCTCATAATTCAAAATGACC+AGG + chr8.1:12722055-12722074 MS.gene036826:intron 30.0%
TGAATTAAATGGCAGAATTC+TGG - chr8.1:12721970-12721989 None:intergenic 30.0%
TTACAAAAAGGCAAAAGGTT+AGG - chr8.1:12720890-12720909 None:intergenic 30.0%
TTAGGAAAAGCTTAAATCGT+TGG - chr8.1:12720872-12720891 None:intergenic 30.0%
! ACCTTTTGCCTTTTTGTAAA+CGG + chr8.1:12720891-12720910 MS.gene036826:CDS 30.0%
! GGTAAGTATTCAGATTTTCT+AGG - chr8.1:12721377-12721396 None:intergenic 30.0%
! GTTAGTGATATTAGGTATTG+CGG - chr8.1:12721806-12721825 None:intergenic 30.0%
! TTAGTGATATTAGGTATTGC+GGG - chr8.1:12721805-12721824 None:intergenic 30.0%
! TTATATAAAGTGCTAAGGGA+TGG + chr8.1:12722467-12722486 MS.gene036826:CDS 30.0%
!! AGAAAATTTGAGTTGAACCA+CGG - chr8.1:12722009-12722028 None:intergenic 30.0%
!!! GTATTCAGATTTTCTAGGAT+AGG - chr8.1:12721372-12721391 None:intergenic 30.0%
AAAACACGCCGTTTACAAAA+AGG - chr8.1:12720902-12720921 None:intergenic 35.0%
AACCTTACCCAAATCAGATT+TGG + chr8.1:12721519-12721538 MS.gene036826:CDS 35.0%
AGTTTCAACTTCTTCAGTGA+TGG - chr8.1:12721303-12721322 None:intergenic 35.0%
ATCCTTTACTCTACTATCCT+AGG - chr8.1:12721835-12721854 None:intergenic 35.0%
ATTAACATGTACAGTCTTGG+TGG + chr8.1:12721234-12721253 MS.gene036826:intron 35.0%
ATTTGCAAACTCTTCAACGT+AGG - chr8.1:12721435-12721454 None:intergenic 35.0%
GAAATGAATGCATTCCTTGT+TGG + chr8.1:12721342-12721361 MS.gene036826:CDS 35.0%
GCAGATGTTCAATAGATACA+TGG + chr8.1:12720997-12721016 MS.gene036826:CDS 35.0%
GGGATAAAACATCTATCGTT+GGG + chr8.1:12721654-12721673 MS.gene036826:CDS 35.0%
TACACCACAATTTACTAAGG+TGG + chr8.1:12721266-12721285 MS.gene036826:intron 35.0%
TGAACCACCTTAGTAAATTG+TGG - chr8.1:12721273-12721292 None:intergenic 35.0%
TGGCGTAATCCTCATAATAA+AGG - chr8.1:12721398-12721417 None:intergenic 35.0%
TGGGATAAAACATCTATCGT+TGG + chr8.1:12721653-12721672 MS.gene036826:CDS 35.0%
TTCATCAGAAAAGTCGAGAA+CGG - chr8.1:12720781-12720800 None:intergenic 35.0%
! GAGCTCTAATGTTTGTTCAA+TGG + chr8.1:12722084-12722103 MS.gene036826:intron 35.0%
!! ATTTCAGCCATTTTCATACC+AGG - chr8.1:12721327-12721346 None:intergenic 35.0%
!! TTTCAGCCATTTTCATACCA+GGG - chr8.1:12721326-12721345 None:intergenic 35.0%
AAAGAATGATCACTCGAGGA+AGG - chr8.1:12721594-12721613 None:intergenic 40.0%
ACATTAGAGCTCATCAAACC+TGG - chr8.1:12722076-12722095 None:intergenic 40.0%
AGGTTAAGATAGTGAGGACA+AGG - chr8.1:12721504-12721523 None:intergenic 40.0%
CAGCTCAAGAACAAGAAGAT+AGG + chr8.1:12720640-12720659 MS.gene036826:CDS 40.0%
CGTAGGTGGGAAACAAATAT+TGG - chr8.1:12721418-12721437 None:intergenic 40.0%
CTCATGTACAAAATCTCTCG+TGG + chr8.1:12720962-12720981 MS.gene036826:CDS 40.0%
CTGGAAAATGTAGATCAGCA+GGG - chr8.1:12721951-12721970 None:intergenic 40.0%
GAAACTCCCTGGTATGAAAA+TGG + chr8.1:12721317-12721336 MS.gene036826:CDS 40.0%
GAATGTCGCACTTCTTACTT+AGG - chr8.1:12722439-12722458 None:intergenic 40.0%
GATGTTGATATCGCTTCTGT+TGG + chr8.1:12721456-12721475 MS.gene036826:CDS 40.0%
GCCGTTTACAAAAAGGCAAA+AGG - chr8.1:12720895-12720914 None:intergenic 40.0%
GTCCTAGGATAGTAGAGTAA+AGG + chr8.1:12721830-12721849 MS.gene036826:intron 40.0%
TCGTTGTTGTCAACAAACAG+TGG + chr8.1:12721914-12721933 MS.gene036826:intron 40.0%
TCTGGAAAATGTAGATCAGC+AGG - chr8.1:12721952-12721971 None:intergenic 40.0%
TGGGTAAGGTTAAGATAGTG+AGG - chr8.1:12721510-12721529 None:intergenic 40.0%
TTAAGATAGTGAGGACAAGG+CGG - chr8.1:12721501-12721520 None:intergenic 40.0%
TTCCTCGAGTGATCATTCTT+TGG + chr8.1:12721593-12721612 MS.gene036826:CDS 40.0%
TTGGAAGGAAAGACATGATG+TGG - chr8.1:12720695-12720714 None:intergenic 40.0%
! ATTTGGAACTCATTCTTCCG+TGG + chr8.1:12721989-12722008 MS.gene036826:intron 40.0%
! GCTTGTTTCAACAGAGCTTT+TGG - chr8.1:12720718-12720737 None:intergenic 40.0%
! GTTTCAACAGAGCTTTTGGT+TGG - chr8.1:12720714-12720733 None:intergenic 40.0%
!! CACCAAATCTGATTTGGGTA+AGG - chr8.1:12721524-12721543 None:intergenic 40.0%
!! TTATCCCAGAGAGAGCATTA+AGG - chr8.1:12721641-12721660 None:intergenic 40.0%
AAGAGTCGCCTTTATCAGGA+TGG + chr8.1:12722255-12722274 MS.gene036826:CDS 45.0%
AAGCTCCTTAATGCTCTCTC+TGG + chr8.1:12721633-12721652 MS.gene036826:CDS 45.0%
ACGCATCTTCCGAATTTGGT+AGG - chr8.1:12720940-12720959 None:intergenic 45.0%
AGAGACGCATCTTCCGAATT+TGG - chr8.1:12720944-12720963 None:intergenic 45.0%
AGAGTCGCCTTTATCAGGAT+GGG + chr8.1:12722256-12722275 MS.gene036826:CDS 45.0%
AGCTCCTTAATGCTCTCTCT+GGG + chr8.1:12721634-12721653 MS.gene036826:CDS 45.0%
ATCGTTGGTCCATGTCATCT+TGG - chr8.1:12720857-12720876 None:intergenic 45.0%
ATGTGGCTCAGTATACAGTC+TGG - chr8.1:12720678-12720697 None:intergenic 45.0%
CAAGCATTCTATCTCGCAGA+TGG + chr8.1:12720733-12720752 MS.gene036826:CDS 45.0%
CCTCATACTCGACGATATTG+CGG + chr8.1:12721058-12721077 MS.gene036826:CDS 45.0%
CGCATCTTCCGAATTTGGTA+GGG - chr8.1:12720939-12720958 None:intergenic 45.0%
CTGAAGAAGTTGAAACTCCC+TGG + chr8.1:12721306-12721325 MS.gene036826:intron 45.0%
GCAAACTCTTCAACGTAGGT+GGG - chr8.1:12721431-12721450 None:intergenic 45.0%
GTATGAGGTCAAGTTCCTTG+AGG - chr8.1:12721046-12721065 None:intergenic 45.0%
GTCAACAAACAGTGGCTTCT+GGG + chr8.1:12721922-12721941 MS.gene036826:intron 45.0%
TGACATAGACCAAGACGACA+TGG + chr8.1:12720830-12720849 MS.gene036826:CDS 45.0%
TGCAAACTCTTCAACGTAGG+TGG - chr8.1:12721432-12721451 None:intergenic 45.0%
TGCTTTAGTGCAAGCAGAGA+GGG - chr8.1:12721111-12721130 None:intergenic 45.0%
TGTCAACAAACAGTGGCTTC+TGG + chr8.1:12721921-12721940 MS.gene036826:intron 45.0%
TGTGGCTCAGTATACAGTCT+GGG - chr8.1:12720677-12720696 None:intergenic 45.0%
TGTGTAGCCCATCCTGATAA+AGG - chr8.1:12722266-12722285 None:intergenic 45.0%
TTGCTTTAGTGCAAGCAGAG+AGG - chr8.1:12721112-12721131 None:intergenic 45.0%
! GTTGGGTTGCTCAACAACAA+AGG + chr8.1:12721671-12721690 MS.gene036826:CDS 45.0%
!! CAACAGAGCTTTTGGTTGGA+AGG - chr8.1:12720710-12720729 None:intergenic 45.0%
!!! TTGAGCTGCTTTTTGCCGTT+TGG - chr8.1:12720628-12720647 None:intergenic 45.0%
AGGTCAAGTTCCTTGAGGTC+AGG - chr8.1:12721041-12721060 None:intergenic 50.0%
CAGGAAGAGTCGCCTTTATC+AGG + chr8.1:12722251-12722270 MS.gene036826:intron 50.0%
CATCTTGGTCCATGTCGTCT+TGG - chr8.1:12720842-12720861 None:intergenic 50.0%
CCGCAATATCGTCGAGTATG+AGG - chr8.1:12721061-12721080 None:intergenic 50.0%
CGACATGGACCAAGATGACA+TGG + chr8.1:12720845-12720864 MS.gene036826:CDS 50.0%
GGCCAAAGAATGATCACTCG+AGG - chr8.1:12721598-12721617 None:intergenic 50.0%
TGGCAGAGTCGAACTCCAAA+CGG + chr8.1:12720610-12720629 MS.gene036826:CDS 50.0%
TTTAGTGCAAGCAGAGAGGG+CGG - chr8.1:12721108-12721127 None:intergenic 50.0%
ACATGGGTCCGCTCCATCAT+TGG + chr8.1:12721014-12721033 MS.gene036826:CDS 55.0%
CATCATTGGGCCTGACCTCA+AGG + chr8.1:12721028-12721047 MS.gene036826:CDS 55.0%
CATGGGTCCGCTCCATCATT+GGG + chr8.1:12721015-12721034 MS.gene036826:CDS 55.0%
CTAGGATAGGGCAGCCAACA+AGG - chr8.1:12721359-12721378 None:intergenic 55.0%
CTCGCAGATGGTGCAACGTT+TGG + chr8.1:12720745-12720764 MS.gene036826:CDS 55.0%
CTTGAGGTCAGGCCCAATGA+TGG - chr8.1:12721030-12721049 None:intergenic 55.0%
GTGGTGTCTTTCGTCATCGC+AGG + chr8.1:12722232-12722251 MS.gene036826:intron 55.0%
! GGTCAGGCCCAATGATGGAG+CGG - chr8.1:12721025-12721044 None:intergenic 60.0%
CAGAGAGGGCGGTAGGCATG+TGG - chr8.1:12721097-12721116 None:intergenic 65.0%
GTGCAAGCAGAGAGGGCGGT+AGG - chr8.1:12721104-12721123 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 12720609 12722514 12720609 ID=MS.gene036826
chr8.1 mRNA 12720609 12722514 12720609 ID=MS.gene036826.t1;Parent=MS.gene036826
chr8.1 exon 12720609 12721079 12720609 ID=MS.gene036826.t1.exon1;Parent=MS.gene036826.t1
chr8.1 CDS 12720609 12721079 12720609 ID=cds.MS.gene036826.t1;Parent=MS.gene036826.t1
chr8.1 exon 12721312 12721692 12721312 ID=MS.gene036826.t1.exon2;Parent=MS.gene036826.t1
chr8.1 CDS 12721312 12721692 12721312 ID=cds.MS.gene036826.t1;Parent=MS.gene036826.t1
chr8.1 exon 12722254 12722514 12722254 ID=MS.gene036826.t1.exon3;Parent=MS.gene036826.t1
chr8.1 CDS 12722254 12722514 12722254 ID=cds.MS.gene036826.t1;Parent=MS.gene036826.t1
Gene Sequence

>MS.gene036826

ATGGCAGAGTCGAACTCCAAACGGCAAAAAGCAGCTCAAGAACAAGAAGATAGGATCAGTAGTCTCCCAGACTGTATACTGAGCCACATCATGTCTTTCCTTCCAACCAAAAGCTCTGTTGAAACAAGCATTCTATCTCGCAGATGGTGCAACGTTTGGAAAGACGTCTCCGTTCTCGACTTTTCTGATGAATCCTTTGAATTTGACAAATTTGACGATGATGACATAGACCAAGACGACATGGACCAAGATGACATGGACCAACGATTTAAGCTTTTCCTAACCTTTTGCCTTTTTGTAAACGGCGTGTTTTCTTCTCTCAATAAACCCTACCAAATTCGGAAGATGCGTCTCTCATGTACAAAATCTCTCGTGGACAACAAATTCTGCAGATGTTCAATAGATACATGGGTCCGCTCCATCATTGGGCCTGACCTCAAGGAACTTGACCTCATACTCGACGATATTGCGGTATTTTTCAGCTACCACATGCCTACCGCCCTCTCTGCTTGCACTAAAGCAAATTCAAGTCATTTTTTATAGTAGTATTTTGTTTTTTAATTATTATTAAAATCACGTATATTGCTACTCTTATGTGACATGACACGATGATAACATACTAACAATTAACATGTACAGTCTTGGTGGTGATATATATACACCACAATTTACTAAGGTGGTTCATTGTTTACCATCACTGAAGAAGTTGAAACTCCCTGGTATGAAAATGGCTGAAATGAATGCATTCCTTGTTGGCTGCCCTATCCTAGAAAATCTGAATACTTACCTTTATTATGAGGATTACGCCAATATTTGTTTCCCACCTACGTTGAAGAGTTTGCAAATCGATGTTGATATCGCTTCTGTTGGTGCTTTCTTGTATGACAATCCGCCTTGTCCTCACTATCTTAACCTTACCCAAATCAGATTTGGTGATGTTAGCAACTTACAAAACGTGATGAGAGCGTCTTTTGATGTTTTTCCTTCCTCGAGTGATCATTCTTTGGCCTTCAAATGTTTATTCAAGCTCCTTAATGCTCTCTCTGGGATAAAACATCTATCGTTGGGTTGCTCAACAACAAAGGTATAGTTTATTATCATAGTTGGATATAGATATGATACCAAATTGTAAATGACTGATAAAATGATAAAATGCGTACACACATATATACAAGTCTTAGAATACAATCAATCCCGCAATACCTAATATCACTAACAATAGTCCTAGGATAGTAGAGTAAAGGATGTGTACATTTTACACACATATTAGCTTTCTAATTACAAGCTAATATTACGTCTTACTACTCGTTGTTGTCAACAAACAGTGGCTTCTGGGTGACCCTGCTGATCTACATTTTCCAGAATTCTGCCATTTAATTCATTTGGAACTCATTCTTCCGTGGTTCAACTCAAATTTTCTGTTGAGTCTGCTTCAGACATGTAAGTTCTCATAATTCAAAATGACCAGGTTTGATGAGCTCTAATGTTTGTTCAATGGTTTATCTTGCTTCACGAACACCACTTAATGATGTGAAATTTATCAATTCAATGTTTTAATGAAATAGATTACACAAGAGGCCTCGATTTATTACCATATATGCATGATTACATCTTATGTATGTTGTGGTGTCTTTCGTCATCGCAGGAAGAGTCGCCTTTATCAGGATGGGCTACACAACCAAGTGTTCCTAATTGTATCATATCACACCTAACTTTTATTCAGTTTAAAGGATATCAAGGACTTCCAGATGAGCTATTATTTGTTGAGTATATTTTGAAAAAAGGACTTGTTTTGAAGACAATGATTATTGATGATATTTCATTGGACCTAAGTAAGAAGTGCGACATTCTCAATAAGTTATATAAAGTGCTAAGGGATGGAATTCGCCAACTTATATTTGACTGA
Protein sequence

>MS.gene036826.t1

MAESNSKRQKAAQEQEDRISSLPDCILSHIMSFLPTKSSVETSILSRRWCNVWKDVSVLDFSDESFEFDKFDDDDIDQDDMDQDDMDQRFKLFLTFCLFVNGVFSSLNKPYQIRKMRLSCTKSLVDNKFCRCSIDTWVRSIIGPDLKELDLILDDIAKLKLPGMKMAEMNAFLVGCPILENLNTYLYYEDYANICFPPTLKSLQIDVDIASVGAFLYDNPPCPHYLNLTQIRFGDVSNLQNVMRASFDVFPSSSDHSLAFKCLFKLLNALSGIKHLSLGCSTTKEESPLSGWATQPSVPNCIISHLTFIQFKGYQGLPDELLFVEYILKKGLVLKTMIIDDISLDLSKKCDILNKLYKVLRDGIRQLIFD