AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene039219

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039219.t1 MTR_2g070780 92.669 341 25 0 6 346 1 341 0.0 643
MS.gene039219.t1 MTR_6g034190 58.077 520 194 4 6 510 1 511 0.0 585
MS.gene039219.t1 MTR_2g072240 50.682 513 180 8 6 510 1 448 4.84e-166 477
MS.gene039219.t1 MTR_2g071700 72.586 321 85 1 22 339 18 338 7.88e-160 457
MS.gene039219.t1 MTR_2g071720 76.243 181 27 1 346 510 1 181 3.71e-94 284
MS.gene039219.t1 MTR_2g070820 89.189 111 12 0 280 390 1 111 7.82e-64 204
MS.gene039219.t1 MTR_2g070660 56.853 197 51 3 6 198 1 167 4.00e-60 196
MS.gene039219.t1 MTR_2g070810 95.745 47 2 0 6 52 49 95 3.58e-23 94.4
MS.gene039219.t1 MTR_2g047858 24.330 448 275 15 18 457 111 502 1.52e-15 79.7
MS.gene039219.t1 MTR_8g468470 25.658 456 283 20 21 457 13 431 5.62e-12 68.2
MS.gene039219.t1 MTR_7g095350 27.331 311 193 11 27 328 13 299 5.64e-12 68.6
MS.gene039219.t1 MTR_7g095350 27.331 311 193 11 27 328 21 307 6.07e-12 68.6
MS.gene039219.t1 MTR_7g095350 27.331 311 193 11 27 328 13 299 6.12e-12 68.2
MS.gene039219.t1 MTR_1g016340 22.667 450 287 13 17 457 10 407 6.22e-12 68.2
MS.gene039219.t1 MTR_7g095350 27.331 311 193 11 27 328 13 299 6.50e-12 67.8
MS.gene039219.t1 MTR_1g016340 22.667 450 287 13 17 457 15 412 8.10e-12 67.8
MS.gene039219.t1 MTR_7g095350 27.331 311 193 11 27 328 21 307 8.10e-12 67.8
MS.gene039219.t1 MTR_6g013240 23.762 303 198 10 10 302 60 339 9.06e-12 68.2
MS.gene039219.t1 MTR_8g014180 52.727 55 26 0 6 60 1 55 1.21e-11 60.5
MS.gene039219.t1 MTR_2g082860 56.000 50 22 0 21 70 26 75 1.63e-11 62.0
MS.gene039219.t1 MTR_6g013380 24.242 297 192 10 16 302 61 334 3.39e-11 66.2
MS.gene039219.t1 MTR_7g007370 50.820 61 30 0 6 66 1 61 5.30e-11 64.7
MS.gene039219.t1 MTR_4g024770 41.791 67 39 0 6 72 8 74 5.80e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039219.t1 AT1G80960 27.273 462 253 17 22 457 11 415 5.62e-22 99.0
MS.gene039219.t1 AT1G80960 27.273 462 253 17 22 457 50 454 7.18e-22 99.0
MS.gene039219.t1 AT1G80960 27.273 462 253 17 22 457 50 454 7.18e-22 99.0
MS.gene039219.t1 AT1G80960 27.273 462 253 17 22 457 50 454 7.18e-22 99.0
MS.gene039219.t1 AT1G80960 31.023 303 151 11 22 308 50 310 2.68e-21 96.3
MS.gene039219.t1 AT1G80960 31.023 303 151 11 22 308 50 310 3.03e-21 97.1
MS.gene039219.t1 AT3G59190 24.554 505 286 24 15 467 5 466 8.12e-16 80.5
MS.gene039219.t1 AT1G67390 26.923 286 172 8 21 302 40 292 1.06e-15 80.1
MS.gene039219.t1 AT3G26922 27.241 290 159 8 21 302 13 258 5.74e-15 76.3
MS.gene039219.t1 AT3G26922 27.241 290 159 8 21 302 13 258 5.74e-15 76.3
MS.gene039219.t1 AT1G78760 22.465 503 286 18 11 481 5 435 8.69e-13 70.9
MS.gene039219.t1 AT5G62970 22.845 464 266 17 29 457 10 416 1.23e-12 70.5
MS.gene039219.t1 AT5G41830 25.183 409 231 19 11 384 2 370 1.31e-12 70.5
MS.gene039219.t1 AT3G59190 25.310 403 228 18 15 384 5 367 2.70e-12 68.9
MS.gene039219.t1 AT3G60790 27.451 306 176 14 7 306 40 305 4.75e-12 68.6
MS.gene039219.t1 AT3G49030 24.895 474 271 18 1 457 1 406 5.38e-12 68.2
MS.gene039219.t1 AT3G52680 53.731 67 30 1 1 67 1 66 1.38e-11 66.6
MS.gene039219.t1 AT3G52680 24.524 473 275 18 1 457 1 407 1.91e-11 66.6
MS.gene039219.t1 AT5G22730 22.016 486 276 18 9 457 15 434 3.71e-11 65.9
MS.gene039219.t1 AT5G22730 22.016 486 276 18 9 457 15 434 3.71e-11 65.9
MS.gene039219.t1 AT5G41840 25.769 260 146 9 22 266 14 241 9.94e-11 64.7

Find 100 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAAATCTGTTGAGGATTA+AGG 0.153196 2.1:+27537084 None:intergenic
GTTAAGTACTTAATATTAAA+TGG 0.208632 2.1:-27535491 MS.gene039219:CDS
TGAAATTTGTGATGGCATTT+TGG 0.220106 2.1:-27536706 MS.gene039219:intron
CCTCACACTCTAGGTCAATT+TGG 0.235008 2.1:-27535660 MS.gene039219:intron
TTCTGAATAATATTACAATT+TGG 0.250934 2.1:-27535463 MS.gene039219:CDS
CTTACCCTGGAAGTTTGTTT+TGG 0.254096 2.1:-27535604 MS.gene039219:CDS
TGTGAACGAGCGTAGGATTT+TGG 0.266869 2.1:-27536901 MS.gene039219:CDS
CCTTGATACAAACATTCTTT+AGG 0.277807 2.1:+27535546 None:intergenic
TAAAATGGTTGAAGGAATAA+TGG 0.285325 2.1:-27538031 None:intergenic
GATTCTTGTGATTATCGTTA+TGG 0.307940 2.1:-27537224 MS.gene039219:CDS
GCACGTAGAACCCGAAGTTT+TGG 0.308366 2.1:+27536804 None:intergenic
TCGTAGCAACTGCATGTTTA+CGG 0.321573 2.1:-27535412 MS.gene039219:CDS
CAAAATCTGTTGAGGATTAA+GGG 0.329839 2.1:+27537085 None:intergenic
TTCAGCCAAAACAAACTTCC+AGG 0.338704 2.1:+27535599 None:intergenic
ATAGAGTATATAAGAAAATA+TGG 0.344916 2.1:-27535341 MS.gene039219:CDS
TTTGTCTTTCGTCAAAATTA+AGG 0.347473 2.1:+27537839 None:intergenic
TACAATAATGTTCAAATGCT+TGG 0.353231 2.1:-27536780 MS.gene039219:CDS
AACTTCAATCTTGCAGTGAC+TGG 0.362551 2.1:-27537772 MS.gene039219:CDS
CTTAGAGGCCCCAAAACTTC+GGG 0.365917 2.1:-27536814 MS.gene039219:CDS
AAAATGGTTGAAGGAATAAT+GGG 0.395722 2.1:-27538030 MS.gene039219:CDS
TTGTGCAAGTGGGGATGTTG+TGG 0.396215 2.1:-27537300 MS.gene039219:CDS
TGTGCAAGTGGGGATGTTGT+GGG 0.402090 2.1:-27537299 MS.gene039219:CDS
CAACTCAAGAACCTTAAAAC+TGG 0.403099 2.1:+27537136 None:intergenic
TTATTTGAAGATTCATTAGT+TGG 0.403722 2.1:+27535771 None:intergenic
GTTCTCACAACTTCCGTGTA+AGG 0.409642 2.1:+27537910 None:intergenic
TGCAAGATTGAAGTTTAGTC+TGG 0.413386 2.1:+27537780 None:intergenic
TCATGAAGATCTGATCTTAG+AGG 0.415542 2.1:-27536829 MS.gene039219:CDS
TATTTGAAGATTCATTAGTT+GGG 0.418765 2.1:+27535772 None:intergenic
TCAAATATTGACAATCATAT+TGG 0.420752 2.1:-27537362 MS.gene039219:CDS
CCTAAAGAATGTTTGTATCA+AGG 0.437189 2.1:-27535546 MS.gene039219:CDS
TACAAACATTCTTTAGGTTG+TGG 0.449660 2.1:+27535552 None:intergenic
ATGGTGGTAGCTTTGATGAT+TGG 0.451451 2.1:-27535322 MS.gene039219:CDS
TGGTGGTAGCTTTGATGATT+GGG 0.457946 2.1:-27535321 MS.gene039219:CDS
GGGATGTTGTGGGGTGGATG+AGG 0.463671 2.1:-27537289 MS.gene039219:CDS
GCATTCCTTTCAATCATGTC+TGG 0.464346 2.1:+27537194 None:intergenic
TATCCCAGGTTTCAGATCAA+TGG 0.467659 2.1:+27535361 None:intergenic
CTAAAGAATGTTTGTATCAA+GGG 0.467863 2.1:-27535545 MS.gene039219:CDS
TCTAATGCTACAACTCTTCA+AGG 0.468328 2.1:+27537337 None:intergenic
ATATAAGCTAGAAGTGCATC+AGG 0.471803 2.1:+27537946 None:intergenic
TCCCCACTTGCACAACTCTC+AGG 0.473005 2.1:+27537308 None:intergenic
CCACAATGATTCCCATCGCT+TGG 0.478803 2.1:+27537876 None:intergenic
TTATCCATTGATCTGAAACC+TGG 0.478986 2.1:-27535365 MS.gene039219:CDS
AGGGATACAAATCAGAGTTA+TGG 0.488198 2.1:-27537998 MS.gene039219:CDS
AAAAGGCATAAAATGGTTGA+AGG 0.491307 2.1:-27538039 None:intergenic
ACAACTTCCGTGTAAGGAAG+TGG 0.493916 2.1:+27537916 None:intergenic
AAACTGGAGAAAACTTCAAA+AGG 0.495741 2.1:+27537152 None:intergenic
CATTTCTCCACTTCCTTACA+CGG 0.495992 2.1:-27537923 MS.gene039219:CDS
GTGCAAGTGGGGATGTTGTG+GGG 0.497345 2.1:-27537298 MS.gene039219:CDS
TCTTAGAGGCCCCAAAACTT+CGG 0.498004 2.1:-27536815 MS.gene039219:CDS
GGAATAATGGGTGAAGGAGA+AGG 0.503229 2.1:-27538018 MS.gene039219:CDS
GATAATTTCAGAGAGAGCTT+CGG 0.503369 2.1:+27535383 None:intergenic
CAAGTGGGGATGTTGTGGGG+TGG 0.508240 2.1:-27537295 MS.gene039219:CDS
CAAAACAAACTTCCAGGGTA+AGG 0.518602 2.1:+27535605 None:intergenic
CTTTCGTCAAAATTAAGGCT+AGG 0.519973 2.1:+27537844 None:intergenic
GATATTATTGAAATTTGTGA+TGG 0.520194 2.1:-27536714 MS.gene039219:CDS
TTAAGAAATCACATTTCTCA+AGG 0.522132 2.1:+27536976 None:intergenic
TCAATTCAAAATCGAGGCAA+AGG 0.529641 2.1:+27535843 None:intergenic
TTTCTTATATACTCTATCCC+AGG 0.537281 2.1:+27535347 None:intergenic
TATCCATTGATCTGAAACCT+GGG 0.540917 2.1:-27535364 MS.gene039219:CDS
ATTTGAAGATTCATTAGTTG+GGG 0.541423 2.1:+27535773 None:intergenic
GAATAATGGGTGAAGGAGAA+GGG 0.545422 2.1:-27538017 MS.gene039219:CDS
TGCAGTGACTGGGCAACGTA+AGG 0.547546 2.1:-27537761 MS.gene039219:intron
CAATTCAAAATCGAGGCAAA+GGG 0.555813 2.1:+27535844 None:intergenic
AACTTCAAAAGGTAAATCAA+TGG 0.562125 2.1:+27537163 None:intergenic
TACCGTTCAATTCAAAATCG+AGG 0.564943 2.1:+27535837 None:intergenic
ACTTCAATCTTGCAGTGACT+GGG 0.567133 2.1:-27537771 MS.gene039219:CDS
CAAGTGTGCTATCCAAGCGA+TGG 0.569302 2.1:-27537888 MS.gene039219:CDS
ATGTGATTTCTTAACAGATG+AGG 0.570169 2.1:-27536967 MS.gene039219:CDS
TCAGCCAAAACAAACTTCCA+GGG 0.571326 2.1:+27535600 None:intergenic
TCTTGTGATTATCGTTATGG+AGG 0.571564 2.1:-27537221 MS.gene039219:CDS
TTTGGTTTCTGCATTTAACA+TGG 0.582670 2.1:-27536883 MS.gene039219:CDS
GAATACCAGACATGATTGAA+AGG 0.585171 2.1:-27537199 MS.gene039219:CDS
TTTGTGATGTGAACGAGCGT+AGG 0.592653 2.1:-27536908 MS.gene039219:CDS
AAGTGTGCTATCCAAGCGAT+GGG 0.595727 2.1:-27537887 MS.gene039219:CDS
GGTCATCGAGGTTCTAGCAT+GGG 0.596942 2.1:-27535890 MS.gene039219:CDS
GATACAAATCAGAGTTATGG+AGG 0.599471 2.1:-27537995 MS.gene039219:CDS
GAATCTTCAAATAAATAACC+AGG 0.602091 2.1:-27535762 MS.gene039219:intron
TACTATGATCAAAATGACAA+TGG 0.603700 2.1:-27535635 MS.gene039219:CDS
GTTTGTATCAAGGGATACAA+AGG 0.605257 2.1:-27535536 MS.gene039219:CDS
AATATTTGATGTAACCATCA+TGG 0.606995 2.1:+27537376 None:intergenic
GTTGAAGGAATAATGGGTGA+AGG 0.607734 2.1:-27538024 MS.gene039219:CDS
CCAAGCGATGGGAATCATTG+TGG 0.608200 2.1:-27537876 MS.gene039219:CDS
AAGTACTTAATATTAAATGG+AGG 0.612207 2.1:-27535488 MS.gene039219:CDS
ACAGGTAGATCTTGAAAGTG+AGG 0.613723 2.1:-27537417 MS.gene039219:intron
TTGTGGCTGACACACCTACA+CGG 0.616160 2.1:+27535569 None:intergenic
AATGGCTGTCTTCCTTACCC+TGG 0.616901 2.1:-27535617 MS.gene039219:CDS
ATCTGTTGAGGATTAAGGGA+GGG 0.619665 2.1:+27537089 None:intergenic
AGAGTATTCAAAATCTGTTG+AGG 0.620364 2.1:+27537077 None:intergenic
TGCCTGAGAGTTGTGCAAGT+GGG 0.620485 2.1:-27537310 MS.gene039219:CDS
GCCTGAGAGTTGTGCAAGTG+GGG 0.621594 2.1:-27537309 MS.gene039219:CDS
CAAGAGATGAACAATGAGAG+AGG 0.622832 2.1:+27537009 None:intergenic
GGGTCATCGAGGTTCTAGCA+TGG 0.629061 2.1:-27535891 MS.gene039219:CDS
TGTGGCTGACACACCTACAC+GGG 0.629776 2.1:+27535570 None:intergenic
CTAATGCTACAACTCTTCAA+GGG 0.636034 2.1:+27537338 None:intergenic
GGCTGAAAAGAGACCCGTGT+AGG 0.671792 2.1:-27535583 MS.gene039219:CDS
TTGCCTGAGAGTTGTGCAAG+TGG 0.675075 2.1:-27537311 MS.gene039219:CDS
GAGTATATAAGAAAATATGG+TGG 0.676614 2.1:-27535338 MS.gene039219:CDS
TGATTGTCTTAGGGTCATCG+AGG 0.687412 2.1:-27535902 MS.gene039219:intron
AATCTGTTGAGGATTAAGGG+AGG 0.688199 2.1:+27537088 None:intergenic
TTTGAAGATTCATTAGTTGG+GGG 0.726871 2.1:+27535774 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATAATTTATATA+TGG - chr2.1:27536814-27536833 MS.gene039219:CDS 0.0%
!! AATTCTTTATATAAATTCTT+TGG + chr2.1:27537327-27537346 None:intergenic 10.0%
!! TCAAATATGATATATATTAT+TGG - chr2.1:27535879-27535898 MS.gene039219:CDS 10.0%
!! ATAGAGTATATAAGAAAATA+TGG - chr2.1:27537990-27538009 MS.gene039219:CDS 15.0%
!! ATGATATCTATTTAATACTT+AGG - chr2.1:27537073-27537092 MS.gene039219:CDS 15.0%
!! GTTAAGTACTTAATATTAAA+TGG - chr2.1:27537840-27537859 MS.gene039219:CDS 15.0%
!! TAGATGAACTTTAATTATAA+AGG - chr2.1:27535852-27535871 MS.gene039219:CDS 15.0%
!! TTCTGAATAATATTACAATT+TGG - chr2.1:27537868-27537887 MS.gene039219:CDS 15.0%
!!! CACAAATTTTAATATATTTG+AGG + chr2.1:27536409-27536428 None:intergenic 15.0%
!!! CATCTATTTTATTAGAAAAA+TGG + chr2.1:27535838-27535857 None:intergenic 15.0%
!!! TAATATTACAATTTGGTTTT+TGG - chr2.1:27537875-27537894 MS.gene039219:CDS 15.0%
!!! TATATAAAGAATTGAAGTTT+TGG - chr2.1:27537334-27537353 MS.gene039219:CDS 15.0%
!!! TGTTTTTTACTATAAACTTT+AGG - chr2.1:27535627-27535646 MS.gene039219:CDS 15.0%
!! AAATAATAGATACTCATGAT+AGG + chr2.1:27537268-27537287 None:intergenic 20.0%
!! AAGTACTTAATATTAAATGG+AGG - chr2.1:27537843-27537862 MS.gene039219:CDS 20.0%
!! AGTTCATAGCTTATTATATA+TGG - chr2.1:27537008-27537027 MS.gene039219:CDS 20.0%
!! ATCAAACAGTTCATATTTAA+TGG - chr2.1:27535763-27535782 MS.gene039219:CDS 20.0%
!! CATTTCTTTATAGTTAATAG+AGG - chr2.1:27536709-27536728 MS.gene039219:CDS 20.0%
!! GATATTATTGAAATTTGTGA+TGG - chr2.1:27536617-27536636 MS.gene039219:intron 20.0%
!! TAATCAATATTTGTAGCATA+AGG + chr2.1:27537160-27537179 None:intergenic 20.0%
!! TATTTGAAGATTCATTAGTT+GGG + chr2.1:27537562-27537581 None:intergenic 20.0%
!! TCAAATATTGACAATCATAT+TGG - chr2.1:27535969-27535988 MS.gene039219:intron 20.0%
!! TTATTTGAAGATTCATTAGT+TGG + chr2.1:27537563-27537582 None:intergenic 20.0%
!!! AGGTTTTATTACTATTCAAT+AGG + chr2.1:27537136-27537155 None:intergenic 20.0%
!!! ATTTTAATATATTTGAGGCT+TGG + chr2.1:27536404-27536423 None:intergenic 20.0%
!!! GGTTTTATTACTATTCAATA+GGG + chr2.1:27537135-27537154 None:intergenic 20.0%
!!! TATAGTAATACTACATTTTG+AGG + chr2.1:27537366-27537385 None:intergenic 20.0%
!!! TATATTTTCTGATTGTCTTA+GGG - chr2.1:27537420-27537439 MS.gene039219:intron 20.0%
!!! TTATATTTTCTGATTGTCTT+AGG - chr2.1:27537419-27537438 MS.gene039219:intron 20.0%
! AACTTCAAAAGGTAAATCAA+TGG + chr2.1:27536171-27536190 None:intergenic 25.0%
! AATATTTGATGTAACCATCA+TGG + chr2.1:27535958-27535977 None:intergenic 25.0%
! ACTAATTGAGTATTTGTGAT+AGG - chr2.1:27537031-27537050 MS.gene039219:CDS 25.0%
! ATTTGAAGATTCATTAGTTG+GGG + chr2.1:27537561-27537580 None:intergenic 25.0%
! CTAAAGAATGTTTGTATCAA+GGG - chr2.1:27537786-27537805 MS.gene039219:CDS 25.0%
! GAATCTTCAAATAAATAACC+AGG - chr2.1:27537569-27537588 MS.gene039219:intron 25.0%
! GAGTATATAAGAAAATATGG+TGG - chr2.1:27537993-27538012 MS.gene039219:CDS 25.0%
! TACAATAATGTTCAAATGCT+TGG - chr2.1:27536551-27536570 MS.gene039219:intron 25.0%
! TACTATGATCAAAATGACAA+TGG - chr2.1:27537696-27537715 MS.gene039219:intron 25.0%
! TATTTAATGGAGAATGAACT+AGG - chr2.1:27535776-27535795 MS.gene039219:CDS 25.0%
! TTAAGAAATCACATTTCTCA+AGG + chr2.1:27536358-27536377 None:intergenic 25.0%
! TTTGTCTTTCGTCAAAATTA+AGG + chr2.1:27535495-27535514 None:intergenic 25.0%
!! TAAGCTATGAACTTTTTGTA+TGG + chr2.1:27537001-27537020 None:intergenic 25.0%
!!! GGACAAAATGTTTTTATAGA+TGG - chr2.1:27536572-27536591 MS.gene039219:intron 25.0%
!!! TTTTTTGTGAAGATTAACCA+TGG + chr2.1:27535717-27535736 None:intergenic 25.0%
AAACTGGAGAAAACTTCAAA+AGG + chr2.1:27536182-27536201 None:intergenic 30.0%
AGCCAAAAAATCAAATTGCA+TGG + chr2.1:27537198-27537217 None:intergenic 30.0%
ATGTGATTTCTTAACAGATG+AGG - chr2.1:27536364-27536383 MS.gene039219:intron 30.0%
CAAAATCTGTTGAGGATTAA+GGG + chr2.1:27536249-27536268 None:intergenic 30.0%
CAATATTTGTAGCATAAGGA+AGG + chr2.1:27537156-27537175 None:intergenic 30.0%
CACAAAAAATCACGCTTATT+GGG - chr2.1:27536968-27536987 MS.gene039219:CDS 30.0%
CCTAAAGAATGTTTGTATCA+AGG - chr2.1:27537785-27537804 MS.gene039219:CDS 30.0%
CCTTGATACAAACATTCTTT+AGG + chr2.1:27537788-27537807 None:intergenic 30.0%
GATTCTTGTGATTATCGTTA+TGG - chr2.1:27536107-27536126 MS.gene039219:intron 30.0%
TATTGGACTTTATGCATAAC+AGG - chr2.1:27535896-27535915 MS.gene039219:intron 30.0%
TCAAAATCTGTTGAGGATTA+AGG + chr2.1:27536250-27536269 None:intergenic 30.0%
TCACAAAAAATCACGCTTAT+TGG - chr2.1:27536967-27536986 MS.gene039219:CDS 30.0%
TTTCTTATATACTCTATCCC+AGG + chr2.1:27537987-27538006 None:intergenic 30.0%
TTTGAAGATTCATTAGTTGG+GGG + chr2.1:27537560-27537579 None:intergenic 30.0%
! AGAGTATTCAAAATCTGTTG+AGG + chr2.1:27536257-27536276 None:intergenic 30.0%
! TGAAATTTGTGATGGCATTT+TGG - chr2.1:27536625-27536644 MS.gene039219:intron 30.0%
! TGAAGTTTTCTCCAGTTTTA+AGG - chr2.1:27536184-27536203 MS.gene039219:intron 30.0%
! TTTGGTTTCTGCATTTAACA+TGG - chr2.1:27536448-27536467 MS.gene039219:intron 30.0%
!! GGGAAGTGTCTTTAAAAATT+TGG - chr2.1:27537461-27537480 MS.gene039219:intron 30.0%
!! TACAAACATTCTTTAGGTTG+TGG + chr2.1:27537782-27537801 None:intergenic 30.0%
!!! ATAAGCGTGATTTTTTGTGA+TGG + chr2.1:27536967-27536986 None:intergenic 30.0%
!!! ATTTTTTGTGATGGCTCAAA+AGG + chr2.1:27536958-27536977 None:intergenic 30.0%
!!! CTCCATGCAATTTGATTTTT+TGG - chr2.1:27537193-27537212 MS.gene039219:CDS 30.0%
!!! TTTTTTGTGATGGCTCAAAA+GGG + chr2.1:27536957-27536976 None:intergenic 30.0%
!!! TTTTTTTTGTTGATCGTGTC+TGG - chr2.1:27535669-27535688 MS.gene039219:intron 30.0%
ACTCGAAAAAGAGGTGATTA+AGG - chr2.1:27536070-27536089 MS.gene039219:intron 35.0%
AGGGAATCATACATGCATTA+AGG + chr2.1:27536938-27536957 None:intergenic 35.0%
AGGGATACAAATCAGAGTTA+TGG - chr2.1:27535333-27535352 MS.gene039219:CDS 35.0%
CAACTCAAGAACCTTAAAAC+TGG + chr2.1:27536198-27536217 None:intergenic 35.0%
CAATTCAAAATCGAGGCAAA+GGG + chr2.1:27537490-27537509 None:intergenic 35.0%
CTAATGCTACAACTCTTCAA+GGG + chr2.1:27535996-27536015 None:intergenic 35.0%
CTTTCGTCAAAATTAAGGCT+AGG + chr2.1:27535490-27535509 None:intergenic 35.0%
GAATACCAGACATGATTGAA+AGG - chr2.1:27536132-27536151 MS.gene039219:intron 35.0%
GAGGAAATTACTCGAAAAAG+AGG - chr2.1:27536061-27536080 MS.gene039219:intron 35.0%
GATAATTTCAGAGAGAGCTT+CGG + chr2.1:27537951-27537970 None:intergenic 35.0%
GATACAAATCAGAGTTATGG+AGG - chr2.1:27535336-27535355 MS.gene039219:CDS 35.0%
GTTTGTATCAAGGGATACAA+AGG - chr2.1:27537795-27537814 MS.gene039219:CDS 35.0%
TACCGTTCAATTCAAAATCG+AGG + chr2.1:27537497-27537516 None:intergenic 35.0%
TACTTAGGATGAACACAGAT+TGG - chr2.1:27537088-27537107 MS.gene039219:CDS 35.0%
TCAATTCAAAATCGAGGCAA+AGG + chr2.1:27537491-27537510 None:intergenic 35.0%
TCATGAAGATCTGATCTTAG+AGG - chr2.1:27536502-27536521 MS.gene039219:intron 35.0%
TCTAATGCTACAACTCTTCA+AGG + chr2.1:27535997-27536016 None:intergenic 35.0%
TCTTGTGATTATCGTTATGG+AGG - chr2.1:27536110-27536129 MS.gene039219:intron 35.0%
TGCAAGATTGAAGTTTAGTC+TGG + chr2.1:27535554-27535573 None:intergenic 35.0%
TGCTGCATTACAATTCAACT+AGG - chr2.1:27536759-27536778 MS.gene039219:CDS 35.0%
TTCCAAGTGAAGTATAGTAG+AGG + chr2.1:27536660-27536679 None:intergenic 35.0%
TTCCTCTACTATACTTCACT+TGG - chr2.1:27536655-27536674 MS.gene039219:intron 35.0%
! ATATAAGCTAGAAGTGCATC+AGG + chr2.1:27535388-27535407 None:intergenic 35.0%
! ATGCAAGCTTTTGACAACAA+GGG - chr2.1:27536595-27536614 MS.gene039219:intron 35.0%
! TATCCATTGATCTGAAACCT+GGG - chr2.1:27537967-27537986 MS.gene039219:CDS 35.0%
! TGCCTCGATTTTGAATTGAA+CGG - chr2.1:27537492-27537511 MS.gene039219:intron 35.0%
! TTATCCATTGATCTGAAACC+TGG - chr2.1:27537966-27537985 MS.gene039219:CDS 35.0%
!! ACTATTCAATAGGGTGTTGA+TGG + chr2.1:27537126-27537145 None:intergenic 35.0%
AACTTCAATCTTGCAGTGAC+TGG - chr2.1:27535559-27535578 MS.gene039219:CDS 40.0%
AATCTGTTGAGGATTAAGGG+AGG + chr2.1:27536246-27536265 None:intergenic 40.0%
ACAGGTAGATCTTGAAAGTG+AGG - chr2.1:27535914-27535933 MS.gene039219:intron 40.0%
ACATTTAACCCTCACACTCT+AGG - chr2.1:27537662-27537681 MS.gene039219:intron 40.0%
ACTTCAATCTTGCAGTGACT+GGG - chr2.1:27535560-27535579 MS.gene039219:CDS 40.0%
ATAACCAGGTATGCTTGCTT+AGG - chr2.1:27537583-27537602 MS.gene039219:intron 40.0%
ATCTGTTGAGGATTAAGGGA+GGG + chr2.1:27536245-27536264 None:intergenic 40.0%
CAAAACAAACTTCCAGGGTA+AGG + chr2.1:27537729-27537748 None:intergenic 40.0%
CAAATTGACCTAGAGTGTGA+GGG + chr2.1:27537673-27537692 None:intergenic 40.0%
CAAGAGATGAACAATGAGAG+AGG + chr2.1:27536325-27536344 None:intergenic 40.0%
CATTTCTCCACTTCCTTACA+CGG - chr2.1:27535408-27535427 MS.gene039219:CDS 40.0%
CTATATCTCTAGACAAGCCA+TGG - chr2.1:27535697-27535716 MS.gene039219:intron 40.0%
GCATTCCTTTCAATCATGTC+TGG + chr2.1:27536140-27536159 None:intergenic 40.0%
GGCTTGTCTAGAGATATAGA+GGG + chr2.1:27535696-27535715 None:intergenic 40.0%
TAATCCTAAGCAAGCATACC+TGG + chr2.1:27537590-27537609 None:intergenic 40.0%
TATCCCAGGTTTCAGATCAA+TGG + chr2.1:27537973-27537992 None:intergenic 40.0%
TCAGCCAAAACAAACTTCCA+GGG + chr2.1:27537734-27537753 None:intergenic 40.0%
TCGTAGCAACTGCATGTTTA+CGG - chr2.1:27537919-27537938 MS.gene039219:CDS 40.0%
TTCAGCCAAAACAAACTTCC+AGG + chr2.1:27537735-27537754 None:intergenic 40.0%
! GATGCAAGCTTTTGACAACA+AGG - chr2.1:27536594-27536613 MS.gene039219:intron 40.0%
! TGGCTTGTCTAGAGATATAG+AGG + chr2.1:27535697-27535716 None:intergenic 40.0%
!! ATGGTGGTAGCTTTGATGAT+TGG - chr2.1:27538009-27538028 MS.gene039219:CDS 40.0%
!! CTTACCCTGGAAGTTTGTTT+TGG - chr2.1:27537727-27537746 MS.gene039219:intron 40.0%
!! GAATAATGGGTGAAGGAGAA+GGG - chr2.1:27535314-27535333 MS.gene039219:CDS 40.0%
!! GTTGAAGGAATAATGGGTGA+AGG - chr2.1:27535307-27535326 MS.gene039219:CDS 40.0%
!! GTTTTTGGATGACTGCTCAT+GGG - chr2.1:27537890-27537909 MS.gene039219:CDS 40.0%
!! TGGTGGTAGCTTTGATGATT+GGG - chr2.1:27538010-27538029 MS.gene039219:CDS 40.0%
AAGATTCATTAGTTGGGGGC+AGG + chr2.1:27537556-27537575 None:intergenic 45.0%
AAGTGTGCTATCCAAGCGAT+GGG - chr2.1:27535444-27535463 MS.gene039219:CDS 45.0%
ACAACTTCCGTGTAAGGAAG+TGG + chr2.1:27535418-27535437 None:intergenic 45.0%
CCAAATTGACCTAGAGTGTG+AGG + chr2.1:27537674-27537693 None:intergenic 45.0%
CCTCACACTCTAGGTCAATT+TGG - chr2.1:27537671-27537690 MS.gene039219:intron 45.0%
GTTCTCACAACTTCCGTGTA+AGG + chr2.1:27535424-27535443 None:intergenic 45.0%
TCTTAGAGGCCCCAAAACTT+CGG - chr2.1:27536516-27536535 MS.gene039219:intron 45.0%
TGATTGTCTTAGGGTCATCG+AGG - chr2.1:27537429-27537448 MS.gene039219:intron 45.0%
TTTGTGATGTGAACGAGCGT+AGG - chr2.1:27536423-27536442 MS.gene039219:intron 45.0%
! ACGTAGAACCCGAAGTTTTG+GGG + chr2.1:27536528-27536547 None:intergenic 45.0%
! CACGTAGAACCCGAAGTTTT+GGG + chr2.1:27536529-27536548 None:intergenic 45.0%
! TGTGAACGAGCGTAGGATTT+TGG - chr2.1:27536430-27536449 MS.gene039219:intron 45.0%
! TTTTGCTGAAGCAGCCATGA+TGG - chr2.1:27535941-27535960 MS.gene039219:intron 45.0%
!! GGAATAATGGGTGAAGGAGA+AGG - chr2.1:27535313-27535332 MS.gene039219:CDS 45.0%
!! GGTTTTTGGATGACTGCTCA+TGG - chr2.1:27537889-27537908 MS.gene039219:CDS 45.0%
AATGGCTGTCTTCCTTACCC+TGG - chr2.1:27537714-27537733 MS.gene039219:intron 50.0%
CAAGTGTGCTATCCAAGCGA+TGG - chr2.1:27535443-27535462 MS.gene039219:CDS 50.0%
CCAAGCGATGGGAATCATTG+TGG - chr2.1:27535455-27535474 MS.gene039219:CDS 50.0%
CCACAATGATTCCCATCGCT+TGG + chr2.1:27535458-27535477 None:intergenic 50.0%
CTTAGAGGCCCCAAAACTTC+GGG - chr2.1:27536517-27536536 MS.gene039219:intron 50.0%
TGCCTGAGAGTTGTGCAAGT+GGG - chr2.1:27536021-27536040 MS.gene039219:intron 50.0%
TGTGCAAGTGGGGATGTTGT+GGG - chr2.1:27536032-27536051 MS.gene039219:intron 50.0%
TTGCCTGAGAGTTGTGCAAG+TGG - chr2.1:27536020-27536039 MS.gene039219:intron 50.0%
TTGTGCAAGTGGGGATGTTG+TGG - chr2.1:27536031-27536050 MS.gene039219:intron 50.0%
TTGTGGCTGACACACCTACA+CGG + chr2.1:27537765-27537784 None:intergenic 50.0%
! GCACGTAGAACCCGAAGTTT+TGG + chr2.1:27536530-27536549 None:intergenic 50.0%
! GGTCATCGAGGTTCTAGCAT+GGG - chr2.1:27537441-27537460 MS.gene039219:intron 50.0%
!! TAGGGTGTTGATGGCGTACT+AGG + chr2.1:27537117-27537136 None:intergenic 50.0%
GCCTGAGAGTTGTGCAAGTG+GGG - chr2.1:27536022-27536041 MS.gene039219:intron 55.0%
GGCTGAAAAGAGACCCGTGT+AGG - chr2.1:27537748-27537767 MS.gene039219:intron 55.0%
GTGCAAGTGGGGATGTTGTG+GGG - chr2.1:27536033-27536052 MS.gene039219:intron 55.0%
TCCCCACTTGCACAACTCTC+AGG + chr2.1:27536026-27536045 None:intergenic 55.0%
TGCAGTGACTGGGCAACGTA+AGG - chr2.1:27535570-27535589 MS.gene039219:CDS 55.0%
TGTGGCTGACACACCTACAC+GGG + chr2.1:27537764-27537783 None:intergenic 55.0%
! GGGTCATCGAGGTTCTAGCA+TGG - chr2.1:27537440-27537459 MS.gene039219:intron 55.0%
CAAGTGGGGATGTTGTGGGG+TGG - chr2.1:27536036-27536055 MS.gene039219:intron 60.0%
GGGATGTTGTGGGGTGGATG+AGG - chr2.1:27536042-27536061 MS.gene039219:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 27535304 27538049 27535304 ID=MS.gene039219
chr2.1 mRNA 27535304 27538049 27535304 ID=MS.gene039219.t1;Parent=MS.gene039219
chr2.1 exon 27537762 27538049 27537762 ID=MS.gene039219.t1.exon1;Parent=MS.gene039219.t1
chr2.1 CDS 27537762 27538049 27537762 ID=cds.MS.gene039219.t1;Parent=MS.gene039219.t1
chr2.1 exon 27536707 27537435 27536707 ID=MS.gene039219.t1.exon2;Parent=MS.gene039219.t1
chr2.1 CDS 27536707 27537435 27536707 ID=cds.MS.gene039219.t1;Parent=MS.gene039219.t1
chr2.1 exon 27535763 27535912 27535763 ID=MS.gene039219.t1.exon3;Parent=MS.gene039219.t1
chr2.1 CDS 27535763 27535912 27535763 ID=cds.MS.gene039219.t1;Parent=MS.gene039219.t1
chr2.1 exon 27535304 27535669 27535304 ID=MS.gene039219.t1.exon4;Parent=MS.gene039219.t1
chr2.1 CDS 27535304 27535669 27535304 ID=cds.MS.gene039219.t1;Parent=MS.gene039219.t1
Gene Sequence

>MS.gene039219

ATGGTTGAAGGAATAATGGGTGAAGGAGAAGGGATACAAATCAGAGTTATGGAGGAAGAAGAAGATTTGATCAGCAATCTTCCTGATGCACTTCTAGCTTATATCATTTCTCCACTTCCTTACACGGAAGTTGTGAGAACAAGTGTGCTATCCAAGCGATGGGAATCATTGTGGAAACACACTCCTAGCCTTAATTTTGACGAAAGACAAATGTTAAAGTCATTGATCAAAGAAAACAATCAAAATTCCAGACTAAACTTCAATCTTGCAGTGACTGGGCAACGTAAGGTACATTGTTATAAAAATTTATGTAAAATTTTATGTGTTTTTTACTATAAACTTTAGGTTTTAATTTATGATTGTTTTTTTTTTTGTTGATCGTGTCTGGTCCCTCTATATCTCTAGACAAGCCATGGTTAATCTTCACAAAAAATTATAGATATCTTAATAATTAGATTAATCAAACAGTTCATATTTAATGGAGAATGAACTAGGAAAAATTAGAGAGAATATGATTACATATTTTAGCTTCCATTTTTCTAATAAAATAGATGAACTTTAATTATAAAGGCTTTTCAAATATGATATATATTATTGGACTTTATGCATAACAGGTAGATCTTGAAAGTGAGGAACTTTTTGCTGAAGCAGCCATGATGGTTACATCAAATATTGACAATCATATTGGTCCCTTGAAGAGTTGTAGCATTAGACACTTGCCTGAGAGTTGTGCAAGTGGGGATGTTGTGGGGTGGATGAGGAAATTACTCGAAAAAGAGGTGATTAAGGTTTCAATAGAACGTGATTCTTGTGATTATCGTTATGGAGGAATACCAGACATGATTGAAAGGAATGCTGCATCAACCATTGATTTACCTTTTGAAGTTTTCTCCAGTTTTAAGGTTCTTGAGTTGAAGAACTATCATTTTTTAACTACACCCTCCCTTAATCCTCAACAGATTTTGAATACTCTTACTCTTAACAAAGTGCATATTATATCTAACACTTTTCATGATATCCTCTCTCATTGTTCATCTCTTGAAAATCTCATCCTTGAGAAATGTGATTTCTTAACAGATGAGGTTAAGATTGTAAGTCCAAGCCTCAAATATATTAAAATTTGTGATGTGAACGAGCGTAGGATTTTGGTTTCTGCATTTAACATGGAAGTCATAGAGATTGACTCTGTTACTTGTAATCATGAAGATCTGATCTTAGAGGCCCCAAAACTTCGGGTTCTACGTGCTTACAATAATGTTCAAATGCTTGGACAAAATGTTTTTATAGATGGATGCAAGCTTTTGACAACAAGGGATATTATTGAAATTTGTGATGGCATTTTGGTAAGTGTTTCCTCTACTATACTTCACTTGGAATGTCAGTTTTTTGATTGATGCATATATTACATTTCTTTATAGTTAATAGAGGTGCATGTAATCTAATGTTTTAAATTTTTGCTGCATTACAATTCAACTAGGATATATATGTGTGTGTGTCTATATATATATATATATATATATAATTTATATATGGTTCATATTCAATTACAATTGAATATTACAATCATATGTACATAGATGCAATCATTAAGATTAATACGTGAGTCAATGATAATGATTAAACTTTAATATCCTTAATGCATGTATGATTCCCTTTTGAGCCATCACAAAAAATCACGCTTATTGGGCAATACTCCATACAAAAAGTTCATAGCTTATTATATATGGACTAATTGAGTATTTGTGATAGGATAATAAAACTTGTTGTTTATGATATCTATTTAATACTTAGGATGAACACAGATTGGTAACCTAGTACGCCATCAACACCCTATTGAATAGTAATAAAACCTTCCTTATGCTACAAATATTGATTAGAGTTAGAAAACTCTCCATGCAATTTGATTTTTTGGCTTAGAAATATGTCATGTAAAAAATAGACAATTATATTTTAGTTTTTGACCTATCATGAGTATCTATTATTTAGATCGTTCAGTAATGCTAATTAAATTTTACAGCAACCAAAGAATTTATATAAAGAATTGAAGTTTTGGTTGTATCCTCAAAATGTAGTATTACTATAATTTATGAACATTTATGTCTATTTGTTTTATAATTATATTTTCTGATTGTCTTAGGGTCATCGAGGTTCTAGCATGGGAAGTGTCTTTAAAAATTTGGTTACCCTTTGCCTCGATTTTGAATTGAACGGTAATAGAAATGTCATAGCTCTTTCTTTTGCACTCAAGTCCTGCCCCCAACTAATGAATCTTCAAATAAATAACCAGGTATGCTTGCTTAGGATTATTTTAGCTTGATCATGATTGCAGTCATTATACTTAACTATATATTTTTTTTGACATTTAACCCTCACACTCTAGGTCAATTTGGATTACTATGATCAAAATGACAATGGCTGTCTTCCTTACCCTGGAAGTTTGTTTTGGCTGAAAAGAGACCCGTGTAGGTGTGTCAGCCACAACCTAAAGAATGTTTGTATCAAGGGATACAAAGGTGAAGAGTTTGAAATTGAATTTGTTAAGTACTTAATATTAAATGGAGGAGTTCTGAATAATATTACAATTTGGTTTTTGGATGACTGCTCATGGGATGACGTCGTAGCAACTGCATGTTTACGGTCATATCCGAAGCTCTCTCTGAAATTATCCATTGATCTGAAACCTGGGATAGAGTATATAAGAAAATATGGTGGTAGCTTTGATGATTGGGTCCGAACCCTAAAATAA
Protein sequence

>MS.gene039219.t1

MVEGIMGEGEGIQIRVMEEEEDLISNLPDALLAYIISPLPYTEVVRTSVLSKRWESLWKHTPSLNFDERQMLKSLIKENNQNSRLNFNLAVTGQRKVDLESEELFAEAAMMVTSNIDNHIGPLKSCSIRHLPESCASGDVVGWMRKLLEKEVIKVSIERDSCDYRYGGIPDMIERNAASTIDLPFEVFSSFKVLELKNYHFLTTPSLNPQQILNTLTLNKVHIISNTFHDILSHCSSLENLILEKCDFLTDEVKIVSPSLKYIKICDVNERRILVSAFNMEVIEIDSVTCNHEDLILEAPKLRVLRAYNNVQMLGQNVFIDGCKLLTTRDIIEICDGILGHRGSSMGSVFKNLVTLCLDFELNGNRNVIALSFALKSCPQLMNLQINNQVNLDYYDQNDNGCLPYPGSLFWLKRDPCRCVSHNLKNVCIKGYKGEEFEIEFVKYLILNGGVLNNITIWFLDDCSWDDVVATACLRSYPKLSLKLSIDLKPGIEYIRKYGGSFDDWVRTLK