AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene039265

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039265.t1 MTR_2g072240 79.324 503 49 2 1 503 1 448 0.0 799
MS.gene039265.t1 MTR_6g034190 62.646 514 178 6 1 503 1 511 0.0 612
MS.gene039265.t1 MTR_2g070780 50.997 351 134 6 1 329 1 335 3.25e-104 316
MS.gene039265.t1 MTR_2g071700 50.621 322 146 3 17 327 18 337 9.12e-99 301
MS.gene039265.t1 MTR_2g071720 60.000 185 52 2 335 503 3 181 1.15e-67 215
MS.gene039265.t1 MTR_2g070660 39.024 205 89 4 1 201 1 173 1.46e-34 128
MS.gene039265.t1 MTR_2g070820 55.856 111 39 2 277 377 1 111 2.27e-28 109
MS.gene039265.t1 MTR_0049s0110 22.779 439 274 15 17 450 8 386 1.64e-16 82.0
MS.gene039265.t1 MTR_2g091145 22.440 459 270 12 12 450 16 408 5.37e-13 71.2
MS.gene039265.t1 MTR_1g016340 21.767 464 272 13 12 450 15 412 8.17e-12 67.8
MS.gene039265.t1 MTR_1g016340 21.767 464 272 13 12 450 10 407 8.64e-12 67.4
MS.gene039265.t1 MTR_2g047872 22.078 462 275 15 7 450 13 407 1.82e-11 66.6
MS.gene039265.t1 MTR_4g085730 32.990 97 61 1 386 482 10 102 2.84e-11 61.2
MS.gene039265.t1 MTR_1g013560 22.173 451 280 12 13 450 15 407 7.36e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039265.t1 AT1G80960 24.625 467 269 18 11 450 5 415 3.28e-16 81.3
MS.gene039265.t1 AT1G80960 24.729 461 264 18 17 450 50 454 3.87e-16 81.3
MS.gene039265.t1 AT1G80960 24.729 461 264 18 17 450 50 454 3.87e-16 81.3
MS.gene039265.t1 AT1G80960 24.729 461 264 18 17 450 50 454 3.87e-16 81.3
MS.gene039265.t1 AT1G80960 24.053 449 258 16 17 450 50 430 1.85e-15 79.0
MS.gene039265.t1 AT1G80960 27.946 297 166 10 17 303 50 308 5.32e-14 74.3
MS.gene039265.t1 AT3G58860 22.030 463 260 18 12 439 1 397 2.16e-12 69.7

Find 87 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAGTATATTTGACAATT+TGG 0.187999 2.2:-20475837 MS.gene039265:CDS
AATGGTGATTGGACAAAATT+TGG 0.227687 2.2:-20476602 MS.gene039265:CDS
ATCTTCATTATTACATATAA+TGG 0.231267 2.2:+20476253 None:intergenic
TACGAATAACATGGAGATTT+GGG 0.257566 2.2:+20476216 None:intergenic
TCAAAACATGGTGGGGATTA+GGG 0.259141 2.2:+20476495 None:intergenic
TTCAAAACATGGTGGGGATT+AGG 0.267252 2.2:+20476494 None:intergenic
TTTGAAGATTCTTTAGTTGA+TGG 0.270378 2.2:+20475741 None:intergenic
AATGATCTACAAAATATTAA+TGG 0.277568 2.2:-20476620 MS.gene039265:CDS
CAATTTGTTCTAGGTCAATA+TGG 0.284091 2.2:-20475418 MS.gene039265:intron
ATACGAATAACATGGAGATT+TGG 0.309965 2.2:+20476215 None:intergenic
ACGATTTCAACATTTCTCTT+TGG 0.320819 2.2:+20477298 None:intergenic
CGTACCCAGGAGTCTTGTTT+TGG 0.327329 2.2:-20475344 MS.gene039265:CDS
GCAATGTATTCTAGAAAATA+TGG 0.333077 2.2:-20475081 MS.gene039265:CDS
AAACATCAAAAGGCATAAAA+AGG 0.342778 2.2:+20476573 None:intergenic
GCTAGGAAGCTCTTCACCTT+AGG 0.344889 2.2:+20475031 None:intergenic
GGAGAAAGAAGTGAGAGATA+TGG 0.349881 2.2:-20477469 MS.gene039265:CDS
ACCGATATGATCATCAATAA+TGG 0.349987 2.2:+20476763 None:intergenic
AGTTGCTACAACTTCATTCC+AGG 0.354639 2.2:+20475164 None:intergenic
AACAAAGCCGCTTTAGCAAT+TGG 0.356119 2.2:+20476794 None:intergenic
GACAATTTAAAAGAACGCTT+TGG 0.366399 2.2:+20475123 None:intergenic
AACATTGAAGTCATTGAGAT+TGG 0.370570 2.2:-20476278 MS.gene039265:CDS
ACAATTTAAAAGAACGCTTT+GGG 0.376169 2.2:+20475124 None:intergenic
AATGCATTCGCATATTTAAG+AGG 0.381007 2.2:+20476824 None:intergenic
TGCGAAGCATTGCCAATTCA+AGG 0.385381 2.2:+20477235 None:intergenic
CTAGGAAGCTCTTCACCTTA+GGG 0.401717 2.2:+20475032 None:intergenic
AACTAAAGTCTCTTTGCATA+AGG 0.403491 2.2:-20475287 MS.gene039265:CDS
GTTAAGTACTTAATATTAAA+CGG 0.404659 2.2:-20475231 MS.gene039265:CDS
TTCTATAATTTCTTTCCTAC+CGG 0.405330 2.2:-20477400 MS.gene039265:CDS
ACTAAAGTCTCTTTGCATAA+GGG 0.406294 2.2:-20475286 MS.gene039265:CDS
TTCTATTATTTGTAAAATCT+AGG 0.408559 2.2:+20475798 None:intergenic
GAATCTTCAAATAAATAATC+AGG 0.416291 2.2:-20475729 MS.gene039265:intron
GTGCAAGCGGGGACGTTGTA+AGG 0.417346 2.2:-20476700 MS.gene039265:CDS
ATTGGCAATGCTTCGCAAAC+AGG 0.418348 2.2:-20477229 MS.gene039265:intron
AAATGTTATTTATTTGGAGA+TGG 0.420199 2.2:-20476380 MS.gene039265:CDS
TACAAAATATTAATGGTGAT+TGG 0.421056 2.2:-20476613 MS.gene039265:CDS
AGGGATATTGTTGAAATTTG+CGG 0.426435 2.2:-20476152 MS.gene039265:CDS
ATTTAAAATATTTGAGACTT+GGG 0.433261 2.2:+20476342 None:intergenic
GTTCTCGCAGCATCTGTTGC+CGG 0.433374 2.2:+20477381 None:intergenic
TTGTCCTTTGATCTTAAACC+TGG 0.435514 2.2:-20475105 MS.gene039265:CDS
AAGATTCTTTAGTTGATGGC+TGG 0.437082 2.2:+20475745 None:intergenic
AATGTTATTTATTTGGAGAT+GGG 0.440071 2.2:-20476379 MS.gene039265:CDS
AATTTAAAATATTTGAGACT+TGG 0.442943 2.2:+20476341 None:intergenic
TTATAGAGAAAATTACAATC+TGG 0.457373 2.2:-20475203 MS.gene039265:CDS
TTATGCAAAGAGACTTTAGT+TGG 0.458307 2.2:+20475289 None:intergenic
AAACTAGTTAAAACATCAAA+AGG 0.466646 2.2:+20476563 None:intergenic
GGTAGGAAAGAAATTATAGA+AGG 0.484425 2.2:+20477402 None:intergenic
TGAAGAAACTACTAGAGAAA+GGG 0.486851 2.2:-20476673 MS.gene039265:CDS
CTTTGCATAAGGGGATATAC+AGG 0.492384 2.2:-20475276 MS.gene039265:CDS
TTTCTAGAATACATTGCTCC+AGG 0.492568 2.2:+20475087 None:intergenic
CTAGCGTGCTTTCCCATCGT+TGG 0.494991 2.2:-20477359 MS.gene039265:CDS
TCCATTATTGATGATCATAT+CGG 0.506347 2.2:-20476764 MS.gene039265:CDS
ATGAAGAAACTACTAGAGAA+AGG 0.519391 2.2:-20476674 MS.gene039265:CDS
CTAAAGTCTCTTTGCATAAG+GGG 0.527260 2.2:-20475285 MS.gene039265:CDS
TGCTCCAGGTTTAAGATCAA+AGG 0.527332 2.2:+20475101 None:intergenic
TGTTAGAGAGTTGTGCAAGC+GGG 0.530974 2.2:-20476712 MS.gene039265:CDS
ATGTTATTTATTTGGAGATG+GGG 0.532647 2.2:-20476378 MS.gene039265:CDS
TAAAATCTAGGTCGAGACAG+AGG 0.542160 2.2:+20475810 None:intergenic
TTTGGATCCAATTGCTAAAG+CGG 0.542263 2.2:-20476801 MS.gene039265:CDS
ATAGAAGGTAGAAACACATC+AGG 0.545819 2.2:+20477417 None:intergenic
TTTGCCAAAACAAGACTCCT+GGG 0.556571 2.2:+20475340 None:intergenic
ATGGATTACATTTACAATGG+TGG 0.558749 2.2:-20475399 MS.gene039265:CDS
CAAAACATGGTGGGGATTAG+GGG 0.565200 2.2:+20476496 None:intergenic
ACAAGCATTGTCATTCTGAT+CGG 0.566654 2.2:+20475371 None:intergenic
CATTGCAAATACGAATAACA+TGG 0.566983 2.2:+20476207 None:intergenic
AAGAGTGTTCAAAACATGGT+GGG 0.569522 2.2:+20476487 None:intergenic
CAAATATACTCATGCAACCT+CGG 0.569771 2.2:+20475846 None:intergenic
CAAAACAAGACTCCTGGGTA+CGG 0.587616 2.2:+20475345 None:intergenic
AGAGTGTTCAAAACATGGTG+GGG 0.602712 2.2:+20476488 None:intergenic
GAAGAAACTACTAGAGAAAG+GGG 0.605742 2.2:-20476672 MS.gene039265:CDS
ACAAGTGGGTAACAACCCTA+AGG 0.616412 2.2:-20475047 MS.gene039265:CDS
GAGTAAGAGTGTTCAAAACA+TGG 0.616962 2.2:+20476483 None:intergenic
AATATGGATTACATTTACAA+TGG 0.619659 2.2:-20475402 MS.gene039265:CDS
GTTAGAGAGTTGTGCAAGCG+GGG 0.626391 2.2:-20476711 MS.gene039265:CDS
CGGGAGGCATTTAGCAACAA+GGG 0.628806 2.2:-20476171 MS.gene039265:CDS
GATATTGTTGAAATTTGCGG+TGG 0.630343 2.2:-20476149 MS.gene039265:CDS
AAAATCTAGGTCGAGACAGA+GGG 0.634478 2.2:+20475811 None:intergenic
CCCATCGTTGGAAAACAATG+TGG 0.638374 2.2:-20477347 MS.gene039265:CDS
ATGTTAGAGAGTTGTGCAAG+CGG 0.639861 2.2:-20476713 MS.gene039265:CDS
TCGGGAGGCATTTAGCAACA+AGG 0.641215 2.2:-20476172 MS.gene039265:CDS
TCGCAGCATCTGTTGCCGGT+AGG 0.647583 2.2:+20477385 None:intergenic
ACGAATAACATGGAGATTTG+GGG 0.656142 2.2:+20476217 None:intergenic
CAAGCGGGGACGTTGTAAGG+TGG 0.656244 2.2:-20476697 MS.gene039265:CDS
TAAGAGTGTTCAAAACATGG+TGG 0.660999 2.2:+20476486 None:intergenic
AATGCTTGTTTACCGTACCC+AGG 0.661784 2.2:-20475357 MS.gene039265:CDS
TGGTAAGTGACACACCGACA+TGG 0.666106 2.2:+20475309 None:intergenic
TGCATAAGGGGATATACAGG+TGG 0.667384 2.2:-20475273 MS.gene039265:CDS
ATGTATTCTAGAAAATATGG+TGG 0.691344 2.2:-20475078 MS.gene039265:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATGAAAATTACAATA+GGG + chr2.2:20476604-20476623 None:intergenic 10.0%
!! AAGTTATTTACATAAATTAT+TGG + chr2.2:20476578-20476597 None:intergenic 10.0%
!! AGTTATTTACATAAATTATT+GGG + chr2.2:20476577-20476596 None:intergenic 10.0%
!! TAAATTATGAAAATTACAAT+AGG + chr2.2:20476605-20476624 None:intergenic 10.0%
!!! AAATAAATAACATTTTTCAA+GGG + chr2.2:20476142-20476161 None:intergenic 10.0%
!!! TAAATATAGTTTTTAACATA+TGG + chr2.2:20476844-20476863 None:intergenic 10.0%
!! AATGATCTACAAAATATTAA+TGG - chr2.2:20475907-20475926 MS.gene039265:intron 15.0%
!! AATTATGAAAATTACAATAG+GGG + chr2.2:20476603-20476622 None:intergenic 15.0%
!! ATCTTCATTATTACATATAA+TGG + chr2.2:20476277-20476296 None:intergenic 15.0%
!! GTTAAGTACTTAATATTAAA+CGG - chr2.2:20477296-20477315 MS.gene039265:CDS 15.0%
!! TATTAATCTAATTGTAGTAT+TGG + chr2.2:20475486-20475505 None:intergenic 15.0%
!! TTCTATTATTTGTAAAATCT+AGG + chr2.2:20476732-20476751 None:intergenic 15.0%
!! TTGAAAAAAAAAACATTTCA+TGG + chr2.2:20477092-20477111 None:intergenic 15.0%
!!! AATTTAAAATATTTGAGACT+TGG + chr2.2:20476189-20476208 None:intergenic 15.0%
!!! ATTAATATTTTGTAGATCAT+TGG + chr2.2:20475908-20475927 None:intergenic 15.0%
!!! ATTTAAAATATTTGAGACTT+GGG + chr2.2:20476188-20476207 None:intergenic 15.0%
!!! CAAATAAATAACATTTTTCA+AGG + chr2.2:20476143-20476162 None:intergenic 15.0%
!!! CATCTATTTTATTACAAAAA+GGG + chr2.2:20475620-20475639 None:intergenic 15.0%
!!! CTTGAAAAATGTTATTTATT+TGG - chr2.2:20476141-20476160 MS.gene039265:intron 15.0%
!!! TCATCTATTTTATTACAAAA+AGG + chr2.2:20475621-20475640 None:intergenic 15.0%
!!! TTTTTGATTTGATTTTTTCT+TGG - chr2.2:20475427-20475446 MS.gene039265:intron 15.0%
!!! TTTTTTTTTCAATTTGTTCT+AGG - chr2.2:20477100-20477119 MS.gene039265:intron 15.0%
!! AAACTAGTTAAAACATCAAA+AGG + chr2.2:20475967-20475986 None:intergenic 20.0%
!! GAATCTTCAAATAAATAATC+AGG - chr2.2:20476798-20476817 MS.gene039265:CDS 20.0%
!! TACAAAATATTAATGGTGAT+TGG - chr2.2:20475914-20475933 MS.gene039265:intron 20.0%
!! TGATTAACTGGAATTAATAA+GGG - chr2.2:20476492-20476511 MS.gene039265:CDS 20.0%
!! TGTAAAGAAAAAAGTTAAGT+AGG + chr2.2:20477025-20477044 None:intergenic 20.0%
!! TTATAGAGAAAATTACAATC+TGG - chr2.2:20477324-20477343 MS.gene039265:CDS 20.0%
!! TTGATTAACTGGAATTAATA+AGG - chr2.2:20476491-20476510 MS.gene039265:CDS 20.0%
!!! AAATGTTATTTATTTGGAGA+TGG - chr2.2:20476147-20476166 MS.gene039265:CDS 20.0%
!!! AATATGGATTACATTTACAA+TGG - chr2.2:20477125-20477144 MS.gene039265:intron 20.0%
!!! AATGTTATTTATTTGGAGAT+GGG - chr2.2:20476148-20476167 MS.gene039265:CDS 20.0%
!!! TGATGTTTTAACTAGTTTTA+AGG - chr2.2:20475969-20475988 MS.gene039265:intron 20.0%
!!! TTTAACTAGTTTTAAGGTTT+TGG - chr2.2:20475975-20475994 MS.gene039265:intron 20.0%
! ACAATTTAAAAGAACGCTTT+GGG + chr2.2:20477406-20477425 None:intergenic 25.0%
! CAATAGTTAGGTTAATCAAT+TGG - chr2.2:20475530-20475549 MS.gene039265:intron 25.0%
! CATAGATACATTTGATTAAC+TGG - chr2.2:20476480-20476499 MS.gene039265:CDS 25.0%
! CATGAGTATATTTGACAATT+TGG - chr2.2:20476690-20476709 MS.gene039265:CDS 25.0%
! GTTCAGAACTACTATTAATT+AGG + chr2.2:20477056-20477075 None:intergenic 25.0%
! TTCTATAATTTCTTTCCTAC+CGG - chr2.2:20475127-20475146 MS.gene039265:CDS 25.0%
! TTTGAAGATTCTTTAGTTGA+TGG + chr2.2:20476789-20476808 None:intergenic 25.0%
!! AAACATCAAAAGGCATAAAA+AGG + chr2.2:20475957-20475976 None:intergenic 25.0%
!! AATTGTAGTATTGGAAAGAA+TGG + chr2.2:20475477-20475496 None:intergenic 25.0%
!! ATGTATTCTAGAAAATATGG+TGG - chr2.2:20477449-20477468 MS.gene039265:CDS 25.0%
!! ATGTTATTTATTTGGAGATG+GGG - chr2.2:20476149-20476168 MS.gene039265:CDS 25.0%
!! GCAATGTATTCTAGAAAATA+TGG - chr2.2:20477446-20477465 MS.gene039265:CDS 25.0%
!! TAAATATGCGAATGCATTTT+TGG - chr2.2:20475708-20475727 MS.gene039265:intron 25.0%
!! TCCATTATTGATGATCATAT+CGG - chr2.2:20475763-20475782 MS.gene039265:CDS 25.0%
!!! AAACTATTGACATTGATTCA+TGG + chr2.2:20476450-20476469 None:intergenic 25.0%
!!! AACTATTGACATTGATTCAT+GGG + chr2.2:20476449-20476468 None:intergenic 25.0%
!!! GAAAATTACAATCTGGTTTT+TGG - chr2.2:20477331-20477350 MS.gene039265:CDS 25.0%
!!! TAATCTGTCCTTATTTTCTT+AGG - chr2.2:20476654-20476673 MS.gene039265:CDS 25.0%
!!! TGTTAGTAATTTGTTCTTGT+TGG - chr2.2:20476549-20476568 MS.gene039265:CDS 25.0%
AACATTGAAGTCATTGAGAT+TGG - chr2.2:20476249-20476268 MS.gene039265:CDS 30.0%
AACTAAAGTCTCTTTGCATA+AGG - chr2.2:20477240-20477259 MS.gene039265:CDS 30.0%
AATGCATTCGCATATTTAAG+AGG + chr2.2:20475706-20475725 None:intergenic 30.0%
AATGGTGATTGGACAAAATT+TGG - chr2.2:20475925-20475944 MS.gene039265:intron 30.0%
ACCGATATGATCATCAATAA+TGG + chr2.2:20475767-20475786 None:intergenic 30.0%
ACGATTTCAACATTTCTCTT+TGG + chr2.2:20475232-20475251 None:intergenic 30.0%
ACTAAAGTCTCTTTGCATAA+GGG - chr2.2:20477241-20477260 MS.gene039265:CDS 30.0%
ACTTGGAATTAGATTCTCTA+TGG + chr2.2:20475589-20475608 None:intergenic 30.0%
AGGGATATTGTTGAAATTTG+CGG - chr2.2:20476375-20476394 MS.gene039265:CDS 30.0%
ATAAATAATCAGGTGCGTTT+AGG - chr2.2:20476808-20476827 MS.gene039265:CDS 30.0%
ATACGAATAACATGGAGATT+TGG + chr2.2:20476315-20476334 None:intergenic 30.0%
ATGAAGAAACTACTAGAGAA+AGG - chr2.2:20475853-20475872 MS.gene039265:CDS 30.0%
CAAAAAGGGAAGTAAAAACT+TGG + chr2.2:20475606-20475625 None:intergenic 30.0%
CATTGCAAATACGAATAACA+TGG + chr2.2:20476323-20476342 None:intergenic 30.0%
GACAATTTAAAAGAACGCTT+TGG + chr2.2:20477407-20477426 None:intergenic 30.0%
GCAGTGATATTTCAATAGTT+AGG - chr2.2:20475518-20475537 MS.gene039265:intron 30.0%
GGTAGGAAAGAAATTATAGA+AGG + chr2.2:20475128-20475147 None:intergenic 30.0%
TAAAAATAAGCGCCTTGAAT+TGG - chr2.2:20475280-20475299 MS.gene039265:CDS 30.0%
TACGAATAACATGGAGATTT+GGG + chr2.2:20476314-20476333 None:intergenic 30.0%
TCATACGCAAATCAAAACAT+TGG + chr2.2:20476524-20476543 None:intergenic 30.0%
TGAAGAAACTACTAGAGAAA+GGG - chr2.2:20475854-20475873 MS.gene039265:CDS 30.0%
TGCAATGATGTGAAAAATCT+CGG - chr2.2:20476336-20476355 MS.gene039265:CDS 30.0%
TTATGCAAAGAGACTTTAGT+TGG + chr2.2:20477241-20477260 None:intergenic 30.0%
! ATCTGTCATGCACAAATTTT+AGG + chr2.2:20475682-20475701 None:intergenic 30.0%
! ATGGATTACATTTACAATGG+TGG - chr2.2:20477128-20477147 MS.gene039265:intron 30.0%
! CAATTTGTTCTAGGTCAATA+TGG - chr2.2:20477109-20477128 MS.gene039265:intron 30.0%
! GTTAGTCATATTTTATTGCG+AGG - chr2.2:20476972-20476991 MS.gene039265:intron 30.0%
! TTTTTGATATGATCGTGCAT+AGG - chr2.2:20476208-20476227 MS.gene039265:CDS 30.0%
!!! AGTCTTGTTTTGGCAAAAAA+GGG - chr2.2:20477193-20477212 MS.gene039265:intron 30.0%
ACGAATAACATGGAGATTTG+GGG + chr2.2:20476313-20476332 None:intergenic 35.0%
ATAGAAGGTAGAAACACATC+AGG + chr2.2:20475113-20475132 None:intergenic 35.0%
CAAATATACTCATGCAACCT+CGG + chr2.2:20476684-20476703 None:intergenic 35.0%
CTAAAGTCTCTTTGCATAAG+GGG - chr2.2:20477242-20477261 MS.gene039265:CDS 35.0%
GAAGAAACTACTAGAGAAAG+GGG - chr2.2:20475855-20475874 MS.gene039265:intron 35.0%
GATATTGTTGAAATTTGCGG+TGG - chr2.2:20476378-20476397 MS.gene039265:CDS 35.0%
GCAATGATGTGAAAAATCTC+GGG - chr2.2:20476337-20476356 MS.gene039265:CDS 35.0%
GTTAAGTAGGACTTAAATCG+TGG + chr2.2:20477012-20477031 None:intergenic 35.0%
TTGTCCTTTGATCTTAAACC+TGG - chr2.2:20477422-20477441 MS.gene039265:CDS 35.0%
TTTGGATCCAATTGCTAAAG+CGG - chr2.2:20475726-20475745 MS.gene039265:intron 35.0%
! ACAAGCATTGTCATTCTGAT+CGG + chr2.2:20477159-20477178 None:intergenic 35.0%
! CTAATGCTGCAACTTTTCAA+AGG + chr2.2:20475790-20475809 None:intergenic 35.0%
!! AAGAGTGTTCAAAACATGGT+GGG + chr2.2:20476043-20476062 None:intergenic 35.0%
!! AAGATTCTTTAGTTGATGGC+TGG + chr2.2:20476785-20476804 None:intergenic 35.0%
!! GAGTAAGAGTGTTCAAAACA+TGG + chr2.2:20476047-20476066 None:intergenic 35.0%
!! TAAGAGTGTTCAAAACATGG+TGG + chr2.2:20476044-20476063 None:intergenic 35.0%
!! TTTCTAGAATACATTGCTCC+AGG + chr2.2:20477443-20477462 None:intergenic 35.0%
!!! GAGTCTTGTTTTGGCAAAAA+AGG - chr2.2:20477192-20477211 MS.gene039265:intron 35.0%
AAAATCTAGGTCGAGACAGA+GGG + chr2.2:20476719-20476738 None:intergenic 40.0%
AGTTGCTACAACTTCATTCC+AGG + chr2.2:20477366-20477385 None:intergenic 40.0%
ATGATGTGAAAAATCTCGGG+AGG - chr2.2:20476340-20476359 MS.gene039265:CDS 40.0%
ATGTTAGAGAGTTGTGCAAG+CGG - chr2.2:20475814-20475833 MS.gene039265:CDS 40.0%
CTTTGCATAAGGGGATATAC+AGG - chr2.2:20477251-20477270 MS.gene039265:CDS 40.0%
GGAGAAAGAAGTGAGAGATA+TGG - chr2.2:20475058-20475077 MS.gene039265:CDS 40.0%
TAAAATCTAGGTCGAGACAG+AGG + chr2.2:20476720-20476739 None:intergenic 40.0%
TCGGCGAACCTAAGAAAATA+AGG + chr2.2:20476665-20476684 None:intergenic 40.0%
TTTGCCAAAACAAGACTCCT+GGG + chr2.2:20477190-20477209 None:intergenic 40.0%
! AACAAAGCCGCTTTAGCAAT+TGG + chr2.2:20475736-20475755 None:intergenic 40.0%
! AGAGTGTTCAAAACATGGTG+GGG + chr2.2:20476042-20476061 None:intergenic 40.0%
! CCACATTGTTTTCCAACGAT+GGG + chr2.2:20475183-20475202 None:intergenic 40.0%
! TCAAAACATGGTGGGGATTA+GGG + chr2.2:20476035-20476054 None:intergenic 40.0%
! TCCACATTGTTTTCCAACGA+TGG + chr2.2:20475184-20475203 None:intergenic 40.0%
! TGCTCCAGGTTTAAGATCAA+AGG + chr2.2:20477429-20477448 None:intergenic 40.0%
! TTATTTTCTTAGGTTCGCCG+AGG - chr2.2:20476664-20476683 MS.gene039265:CDS 40.0%
! TTCAAAACATGGTGGGGATT+AGG + chr2.2:20476036-20476055 None:intergenic 40.0%
! TTTTGCCAAAACAAGACTCC+TGG + chr2.2:20477191-20477210 None:intergenic 40.0%
!! ATGGTGGCAGTTTTAACAAG+TGG - chr2.2:20477465-20477484 MS.gene039265:CDS 40.0%
!! TGGTGGCAGTTTTAACAAGT+GGG - chr2.2:20477466-20477485 MS.gene039265:CDS 40.0%
AATGCTTGTTTACCGTACCC+AGG - chr2.2:20477170-20477189 MS.gene039265:intron 45.0%
ACAAGTGGGTAACAACCCTA+AGG - chr2.2:20477480-20477499 MS.gene039265:CDS 45.0%
ATTGGCAATGCTTCGCAAAC+AGG - chr2.2:20475298-20475317 MS.gene039265:CDS 45.0%
CAAAACAAGACTCCTGGGTA+CGG + chr2.2:20477185-20477204 None:intergenic 45.0%
CCCATCGTTGGAAAACAATG+TGG - chr2.2:20475180-20475199 MS.gene039265:CDS 45.0%
GGCAAAAAAGGGAACCATGT+CGG - chr2.2:20477204-20477223 MS.gene039265:intron 45.0%
TGCATAAGGGGATATACAGG+TGG - chr2.2:20477254-20477273 MS.gene039265:CDS 45.0%
TGTTAGAGAGTTGTGCAAGC+GGG - chr2.2:20475815-20475834 MS.gene039265:CDS 45.0%
! CAAAACATGGTGGGGATTAG+GGG + chr2.2:20476034-20476053 None:intergenic 45.0%
!! GGTTTTTGGATGATTGCTCC+TGG - chr2.2:20477345-20477364 MS.gene039265:CDS 45.0%
!! TGCGAAGCATTGCCAATTCA+AGG + chr2.2:20475295-20475314 None:intergenic 45.0%
GTTAGAGAGTTGTGCAAGCG+GGG - chr2.2:20475816-20475835 MS.gene039265:CDS 50.0%
TGGTAAGTGACACACCGACA+TGG + chr2.2:20477221-20477240 None:intergenic 50.0%
!! CGGGAGGCATTTAGCAACAA+GGG - chr2.2:20476356-20476375 MS.gene039265:CDS 50.0%
!! TCGGGAGGCATTTAGCAACA+AGG - chr2.2:20476355-20476374 MS.gene039265:CDS 50.0%
!!! CGTACCCAGGAGTCTTGTTT+TGG - chr2.2:20477183-20477202 MS.gene039265:intron 50.0%
CTAGCGTGCTTTCCCATCGT+TGG - chr2.2:20475168-20475187 MS.gene039265:CDS 55.0%
GTTCTCGCAGCATCTGTTGC+CGG + chr2.2:20475149-20475168 None:intergenic 55.0%
CAAGCGGGGACGTTGTAAGG+TGG - chr2.2:20475830-20475849 MS.gene039265:CDS 60.0%
GTGCAAGCGGGGACGTTGTA+AGG - chr2.2:20475827-20475846 MS.gene039265:CDS 60.0%
TCGCAGCATCTGTTGCCGGT+AGG + chr2.2:20475145-20475164 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 20475044 20477505 20475044 ID=MS.gene039265
chr2.2 mRNA 20475044 20477505 20475044 ID=MS.gene039265.t1;Parent=MS.gene039265
chr2.2 exon 20477230 20477505 20477230 ID=MS.gene039265.t1.exon1;Parent=MS.gene039265.t1
chr2.2 CDS 20477230 20477505 20477230 ID=cds.MS.gene039265.t1;Parent=MS.gene039265.t1
chr2.2 exon 20476142 20476849 20476142 ID=MS.gene039265.t1.exon2;Parent=MS.gene039265.t1
chr2.2 CDS 20476142 20476849 20476142 ID=cds.MS.gene039265.t1;Parent=MS.gene039265.t1
chr2.2 exon 20475730 20475873 20475730 ID=MS.gene039265.t1.exon3;Parent=MS.gene039265.t1
chr2.2 CDS 20475730 20475873 20475730 ID=cds.MS.gene039265.t1;Parent=MS.gene039265.t1
chr2.2 exon 20475044 20475427 20475044 ID=MS.gene039265.t1.exon4;Parent=MS.gene039265.t1
chr2.2 CDS 20475044 20475427 20475044 ID=cds.MS.gene039265.t1;Parent=MS.gene039265.t1
Gene Sequence

>MS.gene039265

ATGGGGGAAGGAGTGGAGAAAGAAGTGAGAGATATGGATGAAGAAGAAGATTTGTTCAACAATCTTCCTGATGTGTTTCTACCTTCTATAATTTCTTTCCTACCGGCAACAGATGCTGCGAGAACTAGCGTGCTTTCCCATCGTTGGAAAACAATGTGGAAGTACTCTTCGCAACTGAGTTTTAACCAAAGAGAAATGTTGAAATCGTTGATCAAAGTTCATATTGAAACTTATGATAAAAATAAGCGCCTTGAATTGGCAATGCTTCGCAAACAGGTACACTCTTAGAGAAATCATTTAATAGTATTTACTAATTTTTTCTTACATGTTTTACGATTGTAAGTTAATAAAATGTAATCAATCTCTTTACGTTGAAATAACGATTTTTGATTTGATTTTTTCTTGGTTGACTTAATTCTCTCTAGTTCATCCATTCTTTCCAATACTACAATTAGATTAATAAAAAAGTATAGAGCAGTGATATTTCAATAGTTAGGTTAATCAATTGGTTCAAACTTTGTTTGAGAGTTGTTTAGAAATGACCATAGAGAATCTAATTCCAAGTTTTTACTTCCCTTTTTGTAATAAAATAGATGAACTTCAATTCTTAATTCTTTCGAAATATGATATAGATTCCTAAAATTTGTGCATGACAGATACCTCTTAAATATGCGAATGCATTTTTGGATCCAATTGCTAAAGCGGCTTTGTTGATGAAATCCATTATTGATGATCATATCGGTCCTTTGAAAAGTTGCAGCATTAGACATATGTTAGAGAGTTGTGCAAGCGGGGACGTTGTAAGGTGGATGAAGAAACTACTAGAGAAAGGGGTATTAAACGTTTCATTCGAACTTGAATCCAATGATCTACAAAATATTAATGGTGATTGGACAAAATTTGGTGAAATCCTTTTTATGCCTTTTGATGTTTTAACTAGTTTTAAGGTTTTGGAATTGAAGAACTATTATTTGAAAACTACACTTTCCCCTAATCCCCACCATGTTTTGAACACTCTTACTCTTAACAACGTTCGTGTTGAATCAAATGATTTTGAAGACATTCTCTCTCAATGTTTGTCACTTGAAAATCTCACCCTTGAAAAATGTTATTTATTTGGAGATGGGGTCAAGATTGATAGCCCAAGTCTCAAATATTTTAAATTTTTTGATATGATCGTGCATAGGATGTTGATTTCTGCAACTAACATTGAAGTCATTGAGATTGGAACCATTATATGTAATAATGAAGATCTAATTTTCGAGACCCCAAATCTCCATGTTATTCGTATTTGCAATGATGTGAAAAATCTCGGGAGGCATTTAGCAACAAGGGATATTGTTGAAATTTGCGGTGGCATTTCAGTGAGTTTTTCTTCTCATATCTACAATATTTTAGTTTGCCCATGAATCAATGTCAATAGTTTGTGATTTTTGCATAGATACATTTGATTAACTGGAATTAATAAGGGTATAATCCAATGTTTTGATTTGCGTATGAATCAATGTTAGTAATTTGTTCTTGTTGGAACCCAATAATTTATGTAAATAACTTAACCCCTATTGTAATTTTCATAATTTAACATTATATATTTATGTATATTATTATAATAATCTGTCCTTATTTTCTTAGGTTCGCCGAGGTTGCATGAGTATATTTGACAATTTGGTTACCCTCTGTCTCGACCTAGATTTTACAAATAATAGAAATTTAATAGCTCTTTCGTTTGCACTTAAGTCCAGCCATCAACTAAAGAATCTTCAAATAAATAATCAGGTGCGTTTAGGTTTACTTCAGCCATATGTTAAAAACTATATTTATTTAATGTTACATGTGTTAAATCATACATGTCTTAGACTAAATATGCAATAAAAAAAAATGTCATAATATGAAGATTCTTTTTTCTGTGTGAAAAATGTTGTAGTCGAGTTAGTCATATTTTATTGCGAGGCAACAACAATGTGACCACGATTTAAGTCCTACTTAACTTTTTTCTTTACAGCTTAAGACCTAATTAATAGTAGTTCTGAACTACTTAGCTATATCCATGAAATGTTTTTTTTTTCAATTTGTTCTAGGTCAATATGGATTACATTTACAATGGTGGCGTAACCGATCAGAATGACAATGCTTGTTTACCGTACCCAGGAGTCTTGTTTTGGCAAAAAAGGGAACCATGTCGGTGTGTCACTTACCAACTAAAGTCTCTTTGCATAAGGGGATATACAGGTGGAGAGTTTGAATATGAATTTGTTAAGTACTTAATATTAAACGGTGAAGTTATAGAGAAAATTACAATCTGGTTTTTGGATGATTGCTCCTGGAATGAAGTTGTAGCAACTAATTGTCTGCTTTCATACCCAAAGCGTTCTTTTAAATTGTCCTTTGATCTTAAACCTGGAGCAATGTATTCTAGAAAATATGGTGGCAGTTTTAACAAGTGGGTAACAACCCTAAGGTGA
Protein sequence

>MS.gene039265.t1

MGEGVEKEVRDMDEEEDLFNNLPDVFLPSIISFLPATDAARTSVLSHRWKTMWKYSSQLSFNQREMLKSLIKVHIETYDKNKRLELAMLRKQIPLKYANAFLDPIAKAALLMKSIIDDHIGPLKSCSIRHMLESCASGDVVRWMKKLLEKGVLNVSFELESNDLQNINGDWTKFGEILFMPFDVLTSFKVLELKNYYLKTTLSPNPHHVLNTLTLNNVRVESNDFEDILSQCLSLENLTLEKCYLFGDGVKIDSPSLKYFKFFDMIVHRMLISATNIEVIEIGTIICNNEDLIFETPNLHVIRICNDVKNLGRHLATRDIVEICGGISVRRGCMSIFDNLVTLCLDLDFTNNRNLIALSFALKSSHQLKNLQINNQVNMDYIYNGGVTDQNDNACLPYPGVLFWQKREPCRCVTYQLKSLCIRGYTGGEFEYEFVKYLILNGEVIEKITIWFLDDCSWNEVVATNCLLSYPKRSFKLSFDLKPGAMYSRKYGGSFNKWVTTLR