AlfalfaGEDB | MS.gene040294

M. sativa cultivar XinJiangDaYe / MS.gene040294

KEGG: K02905
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040294.t1	XP_024637125.1	96.7	61	2	0	1	61	1	61	7.60E-24	119

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040294.t1	Q9M7X7	86.9	61	8	0	1	61	1	61	1.0e-21	103.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040294.t1	I3SRL9	96.7	61	2	0	1	61	1	61	5.5e-24	119.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene040294	MS.gene73093	PPI
MS.gene043645	MS.gene040294	PPI
MS.gene043593	MS.gene040294	PPI
MS.gene064353	MS.gene040294	PPI
MS.gene040294	MS.gene043595	PPI
MS.gene035826	MS.gene040294	PPI
MS.gene064351	MS.gene040294	PPI
MS.gene035828	MS.gene040294	PPI
MS.gene040294	MS.gene035825	PPI
MS.gene99872	MS.gene040294	PPI
MS.gene043643	MS.gene040294	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040294.t1	MTR_4g121870	96.721	61	2	0	1	61	137	197	4.71e-38	124
MS.gene040294.t1	MTR_4g121877	95.082	61	3	0	1	61	1	61	4.16e-37	118

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040294.t1	AT3G06700	86.885	61	8	0	1	61	1	61	3.03e-32	105
MS.gene040294.t1	AT3G06700	86.885	61	8	0	1	61	1	61	3.03e-32	105
MS.gene040294.t1	AT3G06700	86.885	61	8	0	1	61	1	61	3.03e-32	105
MS.gene040294.t1	AT3G06680	83.607	61	10	0	1	61	23	83	9.66e-32	105
MS.gene040294.t1	AT3G06680	83.607	61	10	0	1	61	1	61	2.47e-31	103
MS.gene040294.t1	AT3G06680	83.607	61	10	0	1	61	1	61	2.47e-31	103

Find 13 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGGCATCAAGAAGCCAAAA+AGG	0.372719	7.4:+70383228	MS.gene040294:CDS
TCTTGTGAGCCTTGTAAGAC+TGG	0.428046	7.4:-70383205	None:intergenic
TGTGTTTCCTCGCATACCTC+TGG	0.457559	7.4:-70383392	None:intergenic
ATCACCACACTTCCACCAAA+GGG	0.482418	7.4:+70383252	MS.gene040294:CDS
CTGAGTAATACATACCCCTT+TGG	0.488635	7.4:-70383267	None:intergenic
CGCTCACAACCAGTCTTACA+AGG	0.489194	7.4:+70383196	MS.gene040294:CDS
TCTTACAAGGCTCACAAGAA+TGG	0.521704	7.4:+70383209	MS.gene040294:CDS
AGTAATACATACCCCTTTGG+TGG	0.557801	7.4:-70383264	None:intergenic
CATCACCACACTTCCACCAA+AGG	0.580490	7.4:+70383251	MS.gene040294:CDS
TAAGGAACCAGAGGTATGCG+AGG	0.651232	7.4:+70383385	MS.gene040294:CDS
CTTGTAAGACTGGTTGTGAG+CGG	0.674336	7.4:-70383195	None:intergenic
ATACCCCTTTGGTGGAAGTG+TGG	0.685351	7.4:-70383256	None:intergenic
TCACCACACTTCCACCAAAG+GGG	0.701603	7.4:+70383253	MS.gene040294:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTACTTTTATAATTGTAGA+TGG	+	chr7.4:70383350-70383369	MS.gene040294:intron	15.0%
	AGGAAACACAACAAGAAAAA+TGG	+	chr7.4:70383405-70383424	MS.gene040294:CDS	30.0%
!	AGATGGATCCAAAGTTTTTA+AGG	+	chr7.4:70383367-70383386	MS.gene040294:intron	30.0%
!	CTCTGGTTCCTTAAAAACTT+TGG	-	chr7.4:70383378-70383397	None:intergenic	35.0%
!!	CAAAGTTTTTAAGGAACCAG+AGG	+	chr7.4:70383376-70383395	MS.gene040294:CDS	35.0%
	AGTAATACATACCCCTTTGG+TGG	-	chr7.4:70383267-70383286	None:intergenic	40.0%
	ATGGCATCAAGAAGCCAAAA+AGG	+	chr7.4:70383228-70383247	MS.gene040294:CDS	40.0%
	CTGAGTAATACATACCCCTT+TGG	-	chr7.4:70383270-70383289	None:intergenic	40.0%
	TCTTACAAGGCTCACAAGAA+TGG	+	chr7.4:70383209-70383228	MS.gene040294:CDS	40.0%
	ATCACCACACTTCCACCAAA+GGG	+	chr7.4:70383252-70383271	MS.gene040294:CDS	45.0%
!	CTTGTAAGACTGGTTGTGAG+CGG	-	chr7.4:70383198-70383217	None:intergenic	45.0%
!	TCTTGTGAGCCTTGTAAGAC+TGG	-	chr7.4:70383208-70383227	None:intergenic	45.0%
!!	GAAGTGTGGTGATGCCTTTT+TGG	-	chr7.4:70383245-70383264	None:intergenic	45.0%
	ATACCCCTTTGGTGGAAGTG+TGG	-	chr7.4:70383259-70383278	None:intergenic	50.0%
	CATCACCACACTTCCACCAA+AGG	+	chr7.4:70383251-70383270	MS.gene040294:CDS	50.0%
	CGCTCACAACCAGTCTTACA+AGG	+	chr7.4:70383196-70383215	MS.gene040294:CDS	50.0%
	TCACCACACTTCCACCAAAG+GGG	+	chr7.4:70383253-70383272	MS.gene040294:CDS	50.0%
	TGTGTTTCCTCGCATACCTC+TGG	-	chr7.4:70383395-70383414	None:intergenic	50.0%
!	TAAGGAACCAGAGGTATGCG+AGG	+	chr7.4:70383385-70383404	MS.gene040294:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	70383173	70383452	70383173	ID=MS.gene040294
chr7.4	mRNA	70383173	70383452	70383173	ID=MS.gene040294.t1;Parent=MS.gene040294
chr7.4	exon	70383173	70383274	70383173	ID=MS.gene040294.t1.exon1;Parent=MS.gene040294.t1
chr7.4	CDS	70383173	70383274	70383173	ID=cds.MS.gene040294.t1;Parent=MS.gene040294.t1
chr7.4	exon	70383369	70383452	70383369	ID=MS.gene040294.t1.exon2;Parent=MS.gene040294.t1
chr7.4	CDS	70383369	70383452	70383369	ID=cds.MS.gene040294.t1;Parent=MS.gene040294.t1

Gene Sequence	>MS.gene040294 ATGGCGAAGTCGAAGAATCACACCGCTCACAACCAGTCTTACAAGGCTCACAAGAATGGCATCAAGAAGCCAAAAAGGCATCACCACACTTCCACCAAAGGGGTATGTATTACTCAGTTTTCAATTCTTTTATTGATTTTGAAATGATTGATTTCTATTGATGATTAATAATTTTATTTTACTTTTATAATTGTAGATGGATCCAAAGTTTTTAAGGAACCAGAGGTATGCGAGGAAACACAACAAGAAAAATGGTGAAATCGCTTCTGCTGAAGAGTAA
Protein sequence	>MS.gene040294.t1 MAKSKNHTAHNQSYKAHKNGIKKPKRHHHTSTKGMDPKFLRNQRYARKHNKKNGEIASAEE

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