AlfalfaGEDB | MS.gene040295

M. sativa cultivar XinJiangDaYe / MS.gene040295

KEGG: K02905
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040295.t1	XP_004508161.1	98.3	60	1	0	1	60	1	60	2.80E-23	117.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040295.t1	Q9M7X7	85.0	60	9	0	1	60	1	60	1.9e-20	99.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040295.t1	I3SZB6	98.3	60	1	0	1	60	1	60	2.1e-23	117.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene73093	MS.gene040295	PPI
MS.gene043643	MS.gene040295	PPI
MS.gene035826	MS.gene040295	PPI
MS.gene064351	MS.gene040295	PPI
MS.gene035828	MS.gene040295	PPI
MS.gene99872	MS.gene040295	PPI
MS.gene043595	MS.gene040295	PPI
MS.gene043645	MS.gene040295	PPI
MS.gene043593	MS.gene040295	PPI
MS.gene064353	MS.gene040295	PPI
MS.gene035825	MS.gene040295	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040295.t1	MTR_4g121877	98.333	60	1	0	1	60	1	60	7.50e-38	119
MS.gene040295.t1	MTR_4g121870	95.000	60	3	0	1	60	137	196	1.29e-36	120

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040295.t1	AT3G06680	85.000	60	9	1	60	23	82	2.96e-31	103
MS.gene040295.t1	AT3G06700	85.000	60	9	1	60	1	60	4.78e-31	102
MS.gene040295.t1	AT3G06700	85.000	60	9	1	60	1	60	4.78e-31	102
MS.gene040295.t1	AT3G06700	85.000	60	9	1	60	1	60	4.78e-31	102
MS.gene040295.t1	AT3G06680	85.000	60	9	1	60	1	60	6.50e-31	102
MS.gene040295.t1	AT3G06680	85.000	60	9	1	60	1	60	6.50e-31	102

Find 13 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGTGTGGCGGTGCCTCTT+TGG	0.285127	7.4:-70388167	None:intergenic
TATGCTTCCGTGCGTACCTC+TGG	0.442911	7.4:-70388321	None:intergenic
ACCGCCACACTTCCACCAAA+GGG	0.460737	7.4:+70388177	MS.gene040295:CDS
CGCTCACAACCAGTCTTACA+AGG	0.508995	7.4:+70388121	MS.gene040295:CDS
ATGATGAATACATACCCCTT+TGG	0.517971	7.4:-70388192	None:intergenic
CACCGCCACACTTCCACCAA+AGG	0.519273	7.4:+70388176	MS.gene040295:CDS
ATGAATACATACCCCTTTGG+TGG	0.547453	7.4:-70388189	None:intergenic
ATGGCATCAAGAAGCCAAAG+AGG	0.617235	7.4:+70388153	MS.gene040295:CDS
ATACCCCTTTGGTGGAAGTG+TGG	0.622392	7.4:-70388181	None:intergenic
CCCCTTTGGTGGAAGTGTGG+CGG	0.635065	7.4:-70388178	None:intergenic
CCGCCACACTTCCACCAAAG+GGG	0.674155	7.4:+70388178	MS.gene040295:CDS
CTTGTAAGACTGGTTGTGAG+CGG	0.674336	7.4:-70388120	None:intergenic
TGAGGAACCAGAGGTACGCA+CGG	0.675026	7.4:+70388314	MS.gene040295:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGTGATTGATTACTGTAGA+TGG	+	chr7.4:70388279-70388298	MS.gene040295:intron	30.0%
	ATGATGAATACATACCCCTT+TGG	-	chr7.4:70388195-70388214	None:intergenic	35.0%
!	AGATGGATCCAAAGTTTTTG+AGG	+	chr7.4:70388296-70388315	MS.gene040295:intron	35.0%
!	CGGAAGCATAACAAGAAAAA+TGG	+	chr7.4:70388334-70388353	MS.gene040295:CDS	35.0%
!!!	CTTCTTGATGCCATTTTTGT+GGG	-	chr7.4:70388147-70388166	None:intergenic	35.0%
	ATGAATACATACCCCTTTGG+TGG	-	chr7.4:70388192-70388211	None:intergenic	40.0%
	TCTTACAAGGCCCACAAAAA+TGG	+	chr7.4:70388134-70388153	MS.gene040295:CDS	40.0%
!	CTCTGGTTCCTCAAAAACTT+TGG	-	chr7.4:70388307-70388326	None:intergenic	40.0%
!!	CAAAGTTTTTGAGGAACCAG+AGG	+	chr7.4:70388305-70388324	MS.gene040295:CDS	40.0%
!!!	GCTTCTTGATGCCATTTTTG+TGG	-	chr7.4:70388148-70388167	None:intergenic	40.0%
	ATGGCATCAAGAAGCCAAAG+AGG	+	chr7.4:70388153-70388172	MS.gene040295:CDS	45.0%
!	CTTGTAAGACTGGTTGTGAG+CGG	-	chr7.4:70388123-70388142	None:intergenic	45.0%
!!	TTTTGTGGGCCTTGTAAGAC+TGG	-	chr7.4:70388133-70388152	None:intergenic	45.0%
	ATACCCCTTTGGTGGAAGTG+TGG	-	chr7.4:70388184-70388203	None:intergenic	50.0%
	CGCTCACAACCAGTCTTACA+AGG	+	chr7.4:70388121-70388140	MS.gene040295:CDS	50.0%
	ACCGCCACACTTCCACCAAA+GGG	+	chr7.4:70388177-70388196	MS.gene040295:CDS	55.0%
	TATGCTTCCGTGCGTACCTC+TGG	-	chr7.4:70388324-70388343	None:intergenic	55.0%
!	TGAGGAACCAGAGGTACGCA+CGG	+	chr7.4:70388314-70388333	MS.gene040295:CDS	55.0%
	CACCGCCACACTTCCACCAA+AGG	+	chr7.4:70388176-70388195	MS.gene040295:CDS	60.0%
	CCGCCACACTTCCACCAAAG+GGG	+	chr7.4:70388178-70388197	MS.gene040295:CDS	60.0%
	GAAGTGTGGCGGTGCCTCTT+TGG	-	chr7.4:70388170-70388189	None:intergenic	60.0%
!	CCCCTTTGGTGGAAGTGTGG+CGG	-	chr7.4:70388181-70388200	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	70388098	70388378	70388098	ID=MS.gene040295
chr7.4	mRNA	70388098	70388378	70388098	ID=MS.gene040295.t1;Parent=MS.gene040295
chr7.4	exon	70388098	70388199	70388098	ID=MS.gene040295.t1.exon1;Parent=MS.gene040295.t1
chr7.4	CDS	70388098	70388199	70388098	ID=cds.MS.gene040295.t1;Parent=MS.gene040295.t1
chr7.4	exon	70388298	70388378	70388298	ID=MS.gene040295.t1.exon2;Parent=MS.gene040295.t1
chr7.4	CDS	70388298	70388378	70388298	ID=cds.MS.gene040295.t1;Parent=MS.gene040295.t1

Gene Sequence	>MS.gene040295 ATGGCGAAGTCGAAGAATCACACCGCTCACAACCAGTCTTACAAGGCCCACAAAAATGGCATCAAGAAGCCAAAGAGGCACCGCCACACTTCCACCAAAGGGGTATGTATTCATCATTCTTCTCTGATTTTTCAAACGATTGTTCTATTTTGTAATGAGATTTTAATTAATTGTTTATTTATTGTGATTGATTACTGTAGATGGATCCAAAGTTTTTGAGGAACCAGAGGTACGCACGGAAGCATAACAAGAAAAATGGTGAAATTGCAGAAGAAGAGTAA
Protein sequence	>MS.gene040295.t1 MAKSKNHTAHNQSYKAHKNGIKKPKRHRHTSTKGMDPKFLRNQRYARKHNKKNGEIAEEE