Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041836.t1 | XP_004508161.1 | 96.7 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 8.30E-23 | 115.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041836.t1 | Q9M7X7 | 83.3 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 5.6e-20 | 97.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041836.t1 | I3SZB6 | 96.7 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 6.0e-23 | 115.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene064353 | MS.gene041836 | PPI |
| MS.gene035826 | MS.gene041836 | PPI |
| MS.gene064351 | MS.gene041836 | PPI |
| MS.gene035825 | MS.gene041836 | PPI |
| MS.gene73093 | MS.gene041836 | PPI |
| MS.gene043643 | MS.gene041836 | PPI |
| MS.gene041836 | MS.gene043595 | PPI |
| MS.gene035828 | MS.gene041836 | PPI |
| MS.gene99872 | MS.gene041836 | PPI |
| MS.gene043593 | MS.gene041836 | PPI |
| MS.gene043645 | MS.gene041836 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041836.t1 | MTR_4g121877 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 2.43e-37 | 118 |
| MS.gene041836.t1 | MTR_4g121870 | 93.333 | 60 | 4 | 0 | 1 | 60 | 137 | 196 | 4.70e-36 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041836.t1 | AT3G06680 | 83.333 | 60 | 10 | 0 | 1 | 60 | 23 | 82 | 9.38e-31 | 102 |
| MS.gene041836.t1 | AT3G06700 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 1.51e-30 | 101 |
| MS.gene041836.t1 | AT3G06700 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 1.51e-30 | 101 |
| MS.gene041836.t1 | AT3G06700 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 1.51e-30 | 101 |
| MS.gene041836.t1 | AT3G06680 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 2.20e-30 | 101 |
| MS.gene041836.t1 | AT3G06680 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 2.20e-30 | 101 |
Find 0 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGATTAATACATACCCCTT+TGG | + | 32454:21816-21835 | None:intergenic | 30.0% | |
| TCAATCGTTTGAAAAATCAG+AGG | + | 32454:21842-21861 | None:intergenic | 30.0% | |
| CTGTGATTGAATACTGTAGA+TGG | - | 32454:21896-21915 | MS.gene041836:CDS | 35.0% | |
| TCTTACAAAGCTCACAAGAA+TGG | - | 32454:21755-21774 | MS.gene041836:CDS | 35.0% | |
| ! | AGATGGATCCAAAGTTTTTG+AGG | - | 32454:21913-21932 | MS.gene041836:CDS | 35.0% |
| ! | AGGAAGCATGACAAGAAAAA+TGG | - | 32454:21951-21970 | MS.gene041836:CDS | 35.0% |
| ATCGTCACACTTCAACCAAA+GGG | - | 32454:21798-21817 | MS.gene041836:intron | 40.0% | |
| ! | CTCTGGTTCCTCAAAAACTT+TGG | + | 32454:21924-21943 | None:intergenic | 40.0% |
| ! | TCTTGTGAGCTTTGTAAGAC+TGG | + | 32454:21754-21773 | None:intergenic | 40.0% |
| ! | TTTGTAAGACTGGTTGTGAG+CGG | + | 32454:21744-21763 | None:intergenic | 40.0% |
| !! | CAAAGTTTTTGAGGAACCAG+AGG | - | 32454:21922-21941 | MS.gene041836:CDS | 40.0% |
| ATGGCATCAAGAAGCCAAAG+AGG | - | 32454:21774-21793 | MS.gene041836:CDS | 45.0% | |
| CATCGTCACACTTCAACCAA+AGG | - | 32454:21797-21816 | MS.gene041836:intron | 45.0% | |
| TCGTCACACTTCAACCAAAG+GGG | - | 32454:21799-21818 | MS.gene041836:intron | 45.0% | |
| CATGCTTCCTTGCATACCTC+TGG | + | 32454:21941-21960 | None:intergenic | 50.0% | |
| GAAGTGTGACGATGCCTCTT+TGG | + | 32454:21791-21810 | None:intergenic | 50.0% | |
| ! | TGAGGAACCAGAGGTATGCA+AGG | - | 32454:21931-21950 | MS.gene041836:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32454 | gene | 21719 | 21995 | 21719 | ID=MS.gene041836 |
| 32454 | mRNA | 21719 | 21995 | 21719 | ID=MS.gene041836.t1;Parent=MS.gene041836 |
| 32454 | exon | 21894 | 21995 | 21894 | ID=MS.gene041836.t1.exon1;Parent=MS.gene041836.t1 |
| 32454 | CDS | 21894 | 21995 | 21894 | ID=cds.MS.gene041836.t1;Parent=MS.gene041836.t1 |
| 32454 | exon | 21719 | 21799 | 21719 | ID=MS.gene041836.t1.exon2;Parent=MS.gene041836.t1 |
| 32454 | CDS | 21719 | 21799 | 21719 | ID=cds.MS.gene041836.t1;Parent=MS.gene041836.t1 |
| Gene Sequence |
| Protein sequence |
