Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041837.t1 | XP_004508161.1 | 100 | 61 | 0 | 0 | 1 | 61 | 1 | 61 | 1.20E-24 | 121.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041837.t1 | Q9M7X7 | 85.2 | 61 | 9 | 0 | 1 | 61 | 1 | 61 | 3.0e-21 | 101.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041837.t1 | I3SZB6 | 100.0 | 61 | 0 | 0 | 1 | 61 | 1 | 61 | 8.5e-25 | 121.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene035826 | MS.gene041837 | PPI |
| MS.gene064351 | MS.gene041837 | PPI |
| MS.gene035828 | MS.gene041837 | PPI |
| MS.gene99872 | MS.gene041837 | PPI |
| MS.gene043643 | MS.gene041837 | PPI |
| MS.gene043595 | MS.gene041837 | PPI |
| MS.gene043645 | MS.gene041837 | PPI |
| MS.gene043593 | MS.gene041837 | PPI |
| MS.gene064353 | MS.gene041837 | PPI |
| MS.gene035825 | MS.gene041837 | PPI |
| MS.gene73093 | MS.gene041837 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041837.t1 | MTR_4g121877 | 100.000 | 61 | 0 | 0 | 1 | 61 | 1 | 61 | 1.29e-39 | 124 |
| MS.gene041837.t1 | MTR_4g121870 | 95.082 | 61 | 3 | 0 | 1 | 61 | 137 | 197 | 1.25e-37 | 123 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041837.t1 | AT3G06680 | 85.246 | 61 | 9 | 0 | 1 | 61 | 23 | 83 | 3.40e-32 | 106 |
| MS.gene041837.t1 | AT3G06700 | 85.246 | 61 | 9 | 0 | 1 | 61 | 1 | 61 | 3.86e-32 | 105 |
| MS.gene041837.t1 | AT3G06700 | 85.246 | 61 | 9 | 0 | 1 | 61 | 1 | 61 | 3.86e-32 | 105 |
| MS.gene041837.t1 | AT3G06700 | 85.246 | 61 | 9 | 0 | 1 | 61 | 1 | 61 | 3.86e-32 | 105 |
| MS.gene041837.t1 | AT3G06680 | 85.246 | 61 | 9 | 0 | 1 | 61 | 1 | 61 | 8.50e-32 | 104 |
| MS.gene041837.t1 | AT3G06680 | 85.246 | 61 | 9 | 0 | 1 | 61 | 1 | 61 | 8.50e-32 | 104 |
Find 0 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAAAAAATGAGAAAGACA+AGG | + | 32454:28630-28649 | None:intergenic | 25.0% |
| !!! | TTTTTGATGATAACTGTAGA+TGG | - | 32454:28716-28735 | MS.gene041837:intron | 25.0% |
| ! | AGATGGATCCAAAGTTTTTG+AGG | - | 32454:28733-28752 | MS.gene041837:CDS | 35.0% |
| ! | AGGAAGCACAACAAGAAAAA+CGG | - | 32454:28771-28790 | MS.gene041837:CDS | 35.0% |
| AAGAGGAATAATTACCCCCT+TGG | + | 32454:28604-28623 | None:intergenic | 40.0% | |
| TCTTACAAGGCTCACAAGAA+TGG | - | 32454:28543-28562 | MS.gene041837:CDS | 40.0% | |
| TGAGAAAGACAAGGAGAAAG+AGG | + | 32454:28621-28640 | None:intergenic | 40.0% | |
| ! | CTCTGGTTCCTCAAAAACTT+TGG | + | 32454:28744-28763 | None:intergenic | 40.0% |
| !! | CAAAGTTTTTGAGGAACCAG+AGG | - | 32454:28742-28761 | MS.gene041837:CDS | 40.0% |
| AGGAATAATTACCCCCTTGG+TGG | + | 32454:28601-28620 | None:intergenic | 45.0% | |
| ATGGCATCAAGAAGCCAAAG+AGG | - | 32454:28562-28581 | MS.gene041837:CDS | 45.0% | |
| ! | CTTGTAAGACTGGTTGTGAG+CGG | + | 32454:28532-28551 | None:intergenic | 45.0% |
| ! | TCTTGTGAGCCTTGTAAGAC+TGG | + | 32454:28542-28561 | None:intergenic | 45.0% |
| CGCTCACAACCAGTCTTACA+AGG | - | 32454:28530-28549 | MS.gene041837:CDS | 50.0% | |
| TGTGCTTCCTTGCATACCTC+TGG | + | 32454:28761-28780 | None:intergenic | 50.0% | |
| ! | TGAGGAACCAGAGGTATGCA+AGG | - | 32454:28751-28770 | MS.gene041837:CDS | 50.0% |
| ATCGCCACACTTCCACCAAG+GGG | - | 32454:28586-28605 | MS.gene041837:intron | 55.0% | |
| CATCGCCACACTTCCACCAA+GGG | - | 32454:28585-28604 | MS.gene041837:intron | 55.0% | |
| GAAGTGTGGCGATGCCTCTT+TGG | + | 32454:28579-28598 | None:intergenic | 55.0% | |
| TTACCCCCTTGGTGGAAGTG+TGG | + | 32454:28593-28612 | None:intergenic | 55.0% | |
| GCATCGCCACACTTCCACCA+AGG | - | 32454:28584-28603 | MS.gene041837:intron | 60.0% | |
| TCGCCACACTTCCACCAAGG+GGG | - | 32454:28587-28606 | MS.gene041837:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32454 | gene | 28507 | 28818 | 28507 | ID=MS.gene041837 |
| 32454 | mRNA | 28507 | 28818 | 28507 | ID=MS.gene041837.t1;Parent=MS.gene041837 |
| 32454 | exon | 28717 | 28818 | 28717 | ID=MS.gene041837.t1.exon1;Parent=MS.gene041837.t1 |
| 32454 | CDS | 28717 | 28818 | 28717 | ID=cds.MS.gene041837.t1;Parent=MS.gene041837.t1 |
| 32454 | exon | 28507 | 28590 | 28507 | ID=MS.gene041837.t1.exon2;Parent=MS.gene041837.t1 |
| 32454 | CDS | 28507 | 28590 | 28507 | ID=cds.MS.gene041837.t1;Parent=MS.gene041837.t1 |
| Gene Sequence |
| Protein sequence |
