AlfalfaGEDB | MS.gene042297

M. sativa cultivar XinJiangDaYe / MS.gene042297

KEGG: K02905
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042297.t1	XP_004508161.1	98.4	61	1	0	1	61	1	61	9.90E-24	118.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042297.t1	Q9M7X7	83.6	61	10	0	1	61	1	61	2.6e-20	98.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042297.t1	I3SZB6	98.4	61	1	0	1	61	1	61	7.2e-24	118.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene043645	MS.gene042297	PPI
MS.gene043593	MS.gene042297	PPI
MS.gene064353	MS.gene042297	PPI
MS.gene042297	MS.gene043595	PPI
MS.gene043643	MS.gene042297	PPI
MS.gene035826	MS.gene042297	PPI
MS.gene064351	MS.gene042297	PPI
MS.gene035828	MS.gene042297	PPI
MS.gene99872	MS.gene042297	PPI
MS.gene042297	MS.gene035825	PPI
MS.gene042297	MS.gene73093	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042297.t1	MTR_4g121877	98.361	61	1	0	1	61	1	61	1.06e-38	122
MS.gene042297.t1	MTR_4g121870	93.443	61	4	0	1	61	137	197	9.39e-37	121

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042297.t1	AT3G06680	83.607	61	10	1	61	23	83	2.62e-31	104
MS.gene042297.t1	AT3G06700	83.607	61	10	1	61	1	61	3.07e-31	103
MS.gene042297.t1	AT3G06700	83.607	61	10	1	61	1	61	3.07e-31	103
MS.gene042297.t1	AT3G06700	83.607	61	10	1	61	1	61	3.07e-31	103
MS.gene042297.t1	AT3G06680	83.607	61	10	1	61	1	61	5.21e-31	102
MS.gene042297.t1	AT3G06680	83.607	61	10	1	61	1	61	5.21e-31	102

Find 13 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGTGTGGCGATGCCTCTT+TGG	0.338170	8.3:+7184913	None:intergenic
TCTTGTGAGCCTTGTAAGAC+TGG	0.428046	8.3:+7184950	None:intergenic
TGTGCTTCCTTGCATACCTC+TGG	0.438138	8.3:+7184731	None:intergenic
CGCTCACAACCAGTCTTACA+AGG	0.489194	8.3:-7184959	MS.gene042297:CDS
TCTTACAAGGCTCACAAGAA+TGG	0.521704	8.3:-7184946	MS.gene042297:CDS
CATCGCCACACTTCCACCAA+GGG	0.564531	8.3:-7184904	MS.gene042297:CDS
AACAATTTACTCTTCTTCAG+TGG	0.567212	8.3:+7184687	None:intergenic
TGAGGAACCAGAGGTATGCA+AGG	0.602234	8.3:-7184738	MS.gene042297:CDS
GCATCGCCACACTTCCACCA+AGG	0.609525	8.3:-7184905	MS.gene042297:CDS
ATGGCATCAAGAAGCCAAAG+AGG	0.631544	8.3:-7184927	MS.gene042297:CDS
ATCGCCACACTTCCACCAAG+GGG	0.644272	8.3:-7184903	MS.gene042297:CDS
CTTGTAAGACTGGTTGTGAG+CGG	0.674336	8.3:+7184960	None:intergenic
TCGCCACACTTCCACCAAGG+GGG	0.721304	8.3:-7184902	MS.gene042297:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGAAAAAAATGATAAAATA+AGG	+	chr8.3:7184825-7184844	None:intergenic	10.0%
!	ATGATAAAATAAGGAAGACA+AGG	+	chr8.3:7184816-7184835	None:intergenic	25.0%
!!!	TTTTTGTTGATAACTGTAGA+TGG	-	chr8.3:7184902-7184921	MS.gene042297:intron	25.0%
!	AGATGGATCCAAAGTTTTTG+AGG	-	chr8.3:7184919-7184938	MS.gene042297:CDS	35.0%
	TAAGGAAGACAAGGAGAAAG+AGG	+	chr8.3:7184807-7184826	None:intergenic	40.0%
	TCTTACAAGGCTCACAAGAA+TGG	-	chr8.3:7184729-7184748	MS.gene042297:CDS	40.0%
!	CTCTGGTTCCTCAAAAACTT+TGG	+	chr8.3:7184930-7184949	None:intergenic	40.0%
!!	CAAAGTTTTTGAGGAACCAG+AGG	-	chr8.3:7184928-7184947	MS.gene042297:CDS	40.0%
	AAGAGGACTAATTACCCCCT+TGG	+	chr8.3:7184790-7184809	None:intergenic	45.0%
	ATGGCATCAAGAAGCCAAAG+AGG	-	chr8.3:7184748-7184767	MS.gene042297:CDS	45.0%
!	CTTGTAAGACTGGTTGTGAG+CGG	+	chr8.3:7184718-7184737	None:intergenic	45.0%
!	TCTTGTGAGCCTTGTAAGAC+TGG	+	chr8.3:7184728-7184747	None:intergenic	45.0%
	AGGACTAATTACCCCCTTGG+TGG	+	chr8.3:7184787-7184806	None:intergenic	50.0%
	CGCTCACAACCAGTCTTACA+AGG	-	chr8.3:7184716-7184735	MS.gene042297:CDS	50.0%
	TGTGCTTCCTTGCATACCTC+TGG	+	chr8.3:7184947-7184966	None:intergenic	50.0%
!	TGAGGAACCAGAGGTATGCA+AGG	-	chr8.3:7184937-7184956	MS.gene042297:CDS	50.0%
	ATCGCCACACTTCCACCAAG+GGG	-	chr8.3:7184772-7184791	MS.gene042297:intron	55.0%
	CATCGCCACACTTCCACCAA+GGG	-	chr8.3:7184771-7184790	MS.gene042297:intron	55.0%
	GAAGTGTGGCGATGCCTCTT+TGG	+	chr8.3:7184765-7184784	None:intergenic	55.0%
	TTACCCCCTTGGTGGAAGTG+TGG	+	chr8.3:7184779-7184798	None:intergenic	55.0%
	GCATCGCCACACTTCCACCA+AGG	-	chr8.3:7184770-7184789	MS.gene042297:intron	60.0%
	TCGCCACACTTCCACCAAGG+GGG	-	chr8.3:7184773-7184792	MS.gene042297:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	7184693	7185004	7184693	ID=MS.gene042297
chr8.3	mRNA	7184693	7185004	7184693	ID=MS.gene042297.t1;Parent=MS.gene042297
chr8.3	exon	7184903	7185004	7184903	ID=MS.gene042297.t1.exon1;Parent=MS.gene042297.t1
chr8.3	CDS	7184903	7185004	7184903	ID=cds.MS.gene042297.t1;Parent=MS.gene042297.t1
chr8.3	exon	7184693	7184776	7184693	ID=MS.gene042297.t1.exon2;Parent=MS.gene042297.t1
chr8.3	CDS	7184693	7184776	7184693	ID=cds.MS.gene042297.t1;Parent=MS.gene042297.t1

Gene Sequence	>MS.gene042297 ATGGCAAAGTCGAAGAATCACACCGCTCACAACCAGTCTTACAAGGCTCACAAGAATGGCATCAAGAAGCCAAAGAGGCATCGCCACACTTCCACCAAGGGGGTAATTAGTCCTCTTTCTCCTTGTCTTCCTTATTTTATCATTTTTTTCATCGATTTTGAAATGATCAATTTATCATAATTTTAAATGTGATTTTGATGAATAGTTTATTTTTGTTGATAACTGTAGATGGATCCAAAGTTTTTGAGGAACCAGAGGTATGCAAGGAAGCACAACAAGAAAAACAGTGAAATCGCCACTGAAGAAGAGTAA
Protein sequence	>MS.gene042297.t1 MAKSKNHTAHNQSYKAHKNGIKKPKRHRHTSTKGMDPKFLRNQRYARKHNKKNSEIATEEE