Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042298.t1 | XP_004508161.1 | 98.3 | 60 | 1 | 0 | 1 | 60 | 1 | 60 | 2.80E-23 | 117.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042298.t1 | Q9M7X7 | 85.0 | 60 | 9 | 0 | 1 | 60 | 1 | 60 | 1.9e-20 | 99.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042298.t1 | I3SZB6 | 98.3 | 60 | 1 | 0 | 1 | 60 | 1 | 60 | 2.1e-23 | 117.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene035828 | MS.gene042298 | PPI |
| MS.gene99872 | MS.gene042298 | PPI |
| MS.gene043595 | MS.gene042298 | PPI |
| MS.gene043593 | MS.gene042298 | PPI |
| MS.gene043645 | MS.gene042298 | PPI |
| MS.gene064353 | MS.gene042298 | PPI |
| MS.gene064351 | MS.gene042298 | PPI |
| MS.gene035825 | MS.gene042298 | PPI |
| MS.gene73093 | MS.gene042298 | PPI |
| MS.gene043643 | MS.gene042298 | PPI |
| MS.gene035826 | MS.gene042298 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042298.t1 | MTR_4g121877 | 98.333 | 60 | 1 | 0 | 1 | 60 | 1 | 60 | 7.50e-38 | 119 |
| MS.gene042298.t1 | MTR_4g121870 | 95.000 | 60 | 3 | 0 | 1 | 60 | 137 | 196 | 1.29e-36 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042298.t1 | AT3G06680 | 85.000 | 60 | 9 | 0 | 1 | 60 | 23 | 82 | 2.96e-31 | 103 |
| MS.gene042298.t1 | AT3G06700 | 85.000 | 60 | 9 | 0 | 1 | 60 | 1 | 60 | 4.78e-31 | 102 |
| MS.gene042298.t1 | AT3G06700 | 85.000 | 60 | 9 | 0 | 1 | 60 | 1 | 60 | 4.78e-31 | 102 |
| MS.gene042298.t1 | AT3G06700 | 85.000 | 60 | 9 | 0 | 1 | 60 | 1 | 60 | 4.78e-31 | 102 |
| MS.gene042298.t1 | AT3G06680 | 85.000 | 60 | 9 | 0 | 1 | 60 | 1 | 60 | 6.50e-31 | 102 |
| MS.gene042298.t1 | AT3G06680 | 85.000 | 60 | 9 | 0 | 1 | 60 | 1 | 60 | 6.50e-31 | 102 |
Find 11 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTGTGACGATGCCTCTT+TGG | 0.332537 | 8.3:+7180827 | None:intergenic |
| TCTTGTGAGCTTTGTAAGAC+TGG | 0.419661 | 8.3:+7180864 | None:intergenic |
| TATGCTTCCTTGCATACCTC+TGG | 0.420261 | 8.3:+7180677 | None:intergenic |
| ATGATTAATACATACCCCTT+TGG | 0.426914 | 8.3:+7180802 | None:intergenic |
| ATCGTCACACTTCAACCAAA+GGG | 0.488627 | 8.3:-7180817 | MS.gene042298:CDS |
| TCTTACAAAGCTCACAAGAA+TGG | 0.532217 | 8.3:-7180860 | MS.gene042298:CDS |
| CATCGTCACACTTCAACCAA+AGG | 0.595920 | 8.3:-7180818 | MS.gene042298:CDS |
| TGAGGAACCAGAGGTATGCA+AGG | 0.602234 | 8.3:-7180684 | MS.gene042298:CDS |
| ATGGCATCAAGAAGCCAAAG+AGG | 0.631544 | 8.3:-7180841 | MS.gene042298:CDS |
| TTTGTAAGACTGGTTGTGAG+CGG | 0.659865 | 8.3:+7180874 | None:intergenic |
| TCGTCACACTTCAACCAAAG+GGG | 0.708509 | 8.3:-7180816 | MS.gene042298:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGATTAATACATACCCCTT+TGG | + | chr8.3:7180739-7180758 | None:intergenic | 30.0% | |
| TCAATCGTTTGAAAAATCAG+AGG | + | chr8.3:7180765-7180784 | None:intergenic | 30.0% | |
| ! | AGGAAGCATAACAAGAAAAA+TGG | - | chr8.3:7180874-7180893 | MS.gene042298:CDS | 30.0% |
| CTGTGATTGAATACTGTAGA+TGG | - | chr8.3:7180819-7180838 | MS.gene042298:CDS | 35.0% | |
| TCTTACAAAGCTCACAAGAA+TGG | - | chr8.3:7180678-7180697 | MS.gene042298:CDS | 35.0% | |
| ! | AGATGGATCCAAAGTTTTTG+AGG | - | chr8.3:7180836-7180855 | MS.gene042298:CDS | 35.0% |
| ATCGTCACACTTCAACCAAA+GGG | - | chr8.3:7180721-7180740 | MS.gene042298:intron | 40.0% | |
| ! | CTCTGGTTCCTCAAAAACTT+TGG | + | chr8.3:7180847-7180866 | None:intergenic | 40.0% |
| ! | TCTTGTGAGCTTTGTAAGAC+TGG | + | chr8.3:7180677-7180696 | None:intergenic | 40.0% |
| ! | TTTGTAAGACTGGTTGTGAG+CGG | + | chr8.3:7180667-7180686 | None:intergenic | 40.0% |
| !! | CAAAGTTTTTGAGGAACCAG+AGG | - | chr8.3:7180845-7180864 | MS.gene042298:CDS | 40.0% |
| ATGGCATCAAGAAGCCAAAG+AGG | - | chr8.3:7180697-7180716 | MS.gene042298:CDS | 45.0% | |
| CATCGTCACACTTCAACCAA+AGG | - | chr8.3:7180720-7180739 | MS.gene042298:intron | 45.0% | |
| TATGCTTCCTTGCATACCTC+TGG | + | chr8.3:7180864-7180883 | None:intergenic | 45.0% | |
| TCGTCACACTTCAACCAAAG+GGG | - | chr8.3:7180722-7180741 | MS.gene042298:intron | 45.0% | |
| GAAGTGTGACGATGCCTCTT+TGG | + | chr8.3:7180714-7180733 | None:intergenic | 50.0% | |
| ! | TGAGGAACCAGAGGTATGCA+AGG | - | chr8.3:7180854-7180873 | MS.gene042298:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 7180642 | 7180918 | 7180642 | ID=MS.gene042298 |
| chr8.3 | mRNA | 7180642 | 7180918 | 7180642 | ID=MS.gene042298.t1;Parent=MS.gene042298 |
| chr8.3 | exon | 7180817 | 7180918 | 7180817 | ID=MS.gene042298.t1.exon1;Parent=MS.gene042298.t1 |
| chr8.3 | CDS | 7180817 | 7180918 | 7180817 | ID=cds.MS.gene042298.t1;Parent=MS.gene042298.t1 |
| chr8.3 | exon | 7180642 | 7180722 | 7180642 | ID=MS.gene042298.t1.exon2;Parent=MS.gene042298.t1 |
| chr8.3 | CDS | 7180642 | 7180722 | 7180642 | ID=cds.MS.gene042298.t1;Parent=MS.gene042298.t1 |
| Gene Sequence |
| Protein sequence |
