AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042769

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042769.t1 MTR_6g039440 69.152 389 120 0 1 389 162 550 0.0 568
MS.gene042769.t1 MTR_5g062230 68.734 387 121 0 1 387 162 548 0.0 566
MS.gene042769.t1 MTR_6g008560 66.067 389 132 0 1 389 10 398 0.0 553
MS.gene042769.t1 MTR_6g008560 66.067 389 132 0 1 389 166 554 0.0 552
MS.gene042769.t1 MTR_5g094620 66.408 387 130 0 3 389 148 534 0.0 551
MS.gene042769.t1 MTR_4g048460 68.734 387 121 0 3 389 172 558 0.0 551
MS.gene042769.t1 MTR_3g464190 65.296 389 135 0 1 389 166 554 0.0 546
MS.gene042769.t1 MTR_5g073260 65.039 389 136 0 1 389 161 549 0.0 536
MS.gene042769.t1 MTR_5g073200 65.039 389 136 0 1 389 158 546 0.0 525
MS.gene042769.t1 MTR_4g081460 59.640 389 157 0 1 389 171 559 8.71e-174 496
MS.gene042769.t1 MTR_4g048460 68.259 293 93 0 3 295 172 464 1.38e-140 409
MS.gene042769.t1 MTR_8g007515 45.431 394 208 5 1 389 162 553 1.81e-121 363
MS.gene042769.t1 MTR_2g081980 45.547 393 208 6 1 389 164 554 8.85e-120 358
MS.gene042769.t1 MTR_2g082010 44.133 392 214 5 1 389 176 565 3.03e-119 357
MS.gene042769.t1 MTR_2g082060 42.132 394 210 7 1 389 170 550 1.72e-106 324
MS.gene042769.t1 MTR_4g019550 39.950 398 217 6 1 389 171 555 9.29e-97 299
MS.gene042769.t1 MTR_2g082050 39.286 392 200 7 1 389 100 456 1.81e-96 295
MS.gene042769.t1 MTR_7g057340 66.272 169 57 0 221 389 1 169 6.29e-83 251
MS.gene042769.t1 MTR_4g045810 34.456 386 252 1 5 389 193 578 6.77e-77 248
MS.gene042769.t1 MTR_5g094530 56.881 218 58 3 2 217 1 184 1.71e-76 235
MS.gene042769.t1 MTR_7g057390 63.905 169 58 1 221 389 1 166 4.05e-76 233
MS.gene042769.t1 MTR_2g089130 34.699 366 237 2 6 370 180 544 3.45e-74 241
MS.gene042769.t1 MTR_2g089120 34.239 368 237 4 6 370 180 545 3.81e-71 233
MS.gene042769.t1 MTR_2g064425 33.504 391 254 5 2 389 181 568 1.35e-67 224
MS.gene042769.t1 MTR_2g064425 33.504 391 254 5 2 389 196 583 2.17e-67 224
MS.gene042769.t1 MTR_2g065450 32.558 387 256 4 6 389 203 587 7.86e-62 209
MS.gene042769.t1 MTR_4g092020 33.161 386 251 4 5 386 183 565 1.88e-61 207
MS.gene042769.t1 MTR_8g057020 31.606 386 261 3 6 389 202 586 1.99e-61 208
MS.gene042769.t1 MTR_7g050990 60.606 165 54 2 36 200 28 181 2.37e-58 204
MS.gene042769.t1 MTR_7g010710 30.749 387 265 2 6 389 166 552 5.49e-57 195
MS.gene042769.t1 MTR_4g092010 28.462 390 267 5 1 384 173 556 3.99e-53 185
MS.gene042769.t1 MTR_2g064425 40.845 213 122 3 2 212 181 391 1.40e-48 170
MS.gene042769.t1 MTR_6g065040 28.446 341 234 5 32 365 54 391 1.79e-48 169
MS.gene042769.t1 MTR_2g064425 40.845 213 122 3 2 212 196 406 2.14e-48 170
MS.gene042769.t1 MTR_3g063170 28.739 341 233 5 32 365 156 493 7.91e-47 167
MS.gene042769.t1 MTR_3g063120 27.222 360 254 6 32 386 132 488 2.15e-46 166
MS.gene042769.t1 MTR_3g465090 27.697 343 244 3 32 370 156 498 4.17e-46 166
MS.gene042769.t1 MTR_3g015080 47.619 168 50 2 221 388 1 130 6.27e-46 154
MS.gene042769.t1 MTR_6g064980 27.635 351 244 5 32 375 156 503 1.63e-45 164
MS.gene042769.t1 MTR_3g063140 27.746 346 240 5 32 370 156 498 3.17e-44 160
MS.gene042769.t1 MTR_3g052120 28.333 360 250 6 32 386 156 512 2.99e-43 157
MS.gene042769.t1 MTR_2g010960 26.702 382 261 6 18 383 140 518 8.92e-43 156
MS.gene042769.t1 MTR_2g012870 24.311 399 275 11 1 379 353 744 7.48e-23 101
MS.gene042769.t1 MTR_2g012900 24.217 351 230 12 1 323 358 700 5.37e-20 92.4
MS.gene042769.t1 MTR_3g058160 22.977 383 253 13 34 389 412 779 8.04e-19 89.0
MS.gene042769.t1 MTR_3g058160 22.977 383 253 13 34 389 405 772 8.38e-19 89.0
MS.gene042769.t1 MTR_3g058160 22.977 383 253 13 34 389 392 759 9.35e-19 88.6
MS.gene042769.t1 MTR_3g058160 22.977 383 253 13 34 389 201 568 1.10e-18 88.2
MS.gene042769.t1 MTR_2g064295 22.715 383 254 12 34 389 392 759 2.66e-18 87.4
MS.gene042769.t1 MTR_3g058160 23.810 336 226 12 69 389 480 800 4.34e-18 86.7
MS.gene042769.t1 MTR_3g063160 32.090 134 89 2 32 164 156 288 3.27e-16 79.0
MS.gene042769.t1 MTR_5g081730 41.573 89 52 0 5 93 23 111 5.50e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042769.t1 AT5G23960 40.519 385 228 1 3 387 157 540 2.10e-107 326
MS.gene042769.t1 AT5G23960 40.464 388 226 3 3 387 157 542 3.47e-106 323
MS.gene042769.t1 AT1G70080 38.046 389 235 3 6 388 219 607 1.43e-93 293
MS.gene042769.t1 AT3G14490 38.974 390 232 3 5 389 210 598 6.16e-92 288
MS.gene042769.t1 AT1G31950 38.046 389 236 2 6 389 216 604 7.65e-92 288
MS.gene042769.t1 AT1G31950 38.046 389 236 2 6 389 215 603 9.14e-92 288
MS.gene042769.t1 AT3G14520 37.018 389 240 2 6 389 213 601 6.99e-91 285
MS.gene042769.t1 AT3G14540 37.789 389 237 2 6 389 210 598 5.23e-89 281
MS.gene042769.t1 AT5G48110 39.039 333 193 5 51 382 1 324 6.11e-86 265
MS.gene042769.t1 AT3G29410 36.269 386 244 2 6 389 110 495 2.39e-85 268
MS.gene042769.t1 AT4G20200 35.844 385 243 3 8 388 216 600 8.30e-85 270
MS.gene042769.t1 AT4G20210 35.052 388 248 3 6 389 210 597 4.66e-84 268
MS.gene042769.t1 AT3G29410 36.269 386 244 2 6 389 215 600 6.12e-84 267
MS.gene042769.t1 AT5G44630 35.825 388 245 3 6 389 167 554 2.37e-83 265
MS.gene042769.t1 AT3G32030 34.286 385 250 2 8 389 217 601 1.43e-82 264
MS.gene042769.t1 AT3G29190 34.961 389 241 5 8 388 213 597 2.53e-81 261
MS.gene042769.t1 AT1G33750 34.197 386 251 2 6 389 216 600 6.26e-81 259
MS.gene042769.t1 AT4G13280 35.401 387 244 5 8 389 166 551 3.53e-80 256
MS.gene042769.t1 AT4G13300 35.233 386 246 3 8 389 166 551 5.18e-80 256
MS.gene042769.t1 AT2G23230 34.974 386 247 4 6 387 210 595 6.54e-76 246
MS.gene042769.t1 AT4G15870 34.021 388 238 5 6 388 220 594 3.01e-75 244
MS.gene042769.t1 AT1G31950 39.739 307 180 2 6 307 216 522 2.31e-74 240
MS.gene042769.t1 AT4G20230 33.679 386 251 4 8 388 216 601 1.47e-73 240
MS.gene042769.t1 AT4G20230 33.679 386 251 4 8 388 228 613 1.74e-73 240
MS.gene042769.t1 AT2G23230 34.715 386 243 5 6 387 210 590 2.11e-73 240
MS.gene042769.t1 AT1G66020 34.026 385 250 3 8 388 210 594 2.46e-72 237
MS.gene042769.t1 AT3G14540 39.933 298 175 1 6 299 210 507 3.68e-72 234
MS.gene042769.t1 AT3G29110 34.286 385 249 4 8 388 209 593 1.22e-70 233
MS.gene042769.t1 AT4G13280 33.938 386 221 5 8 389 166 521 6.28e-69 226
MS.gene042769.t1 AT1G48800 31.783 387 257 5 8 388 217 602 6.90e-67 223
MS.gene042769.t1 AT4G16730 31.856 361 237 5 35 389 133 490 8.71e-65 214
MS.gene042769.t1 AT4G16730 31.856 361 237 5 35 389 179 536 2.61e-64 214
MS.gene042769.t1 AT1G48820 31.250 384 241 6 8 385 191 557 1.12e-62 211
MS.gene042769.t1 AT1G61680 30.749 387 263 2 1 382 176 562 3.69e-60 204
MS.gene042769.t1 AT4G16740 32.692 364 238 6 28 389 203 561 4.82e-60 204
MS.gene042769.t1 AT1G61680 30.749 387 263 2 1 382 183 569 4.98e-60 204
MS.gene042769.t1 AT3G25810 31.805 349 232 3 47 389 246 594 5.33e-58 199
MS.gene042769.t1 AT2G24210 31.856 361 239 6 35 389 228 587 5.63e-58 199
MS.gene042769.t1 AT3G25830 32.000 350 229 6 47 389 244 591 1.41e-55 192
MS.gene042769.t1 AT3G25820 32.000 350 229 6 47 389 244 591 1.41e-55 192
MS.gene042769.t1 AT1G66020 38.208 212 127 3 8 215 210 421 6.12e-42 152
MS.gene042769.t1 AT4G16740 37.895 190 117 1 28 217 203 391 7.13e-40 146
MS.gene042769.t1 AT3G25820 42.177 147 82 1 47 193 244 387 1.43e-36 138
MS.gene042769.t1 AT4G16740 38.849 139 85 0 28 166 203 341 5.43e-29 116
MS.gene042769.t1 AT4G16740 38.849 139 85 0 28 166 203 341 5.43e-29 116
MS.gene042769.t1 AT3G25820 40.833 120 71 0 47 166 244 363 7.35e-29 116
MS.gene042769.t1 AT3G25820 40.833 120 71 0 47 166 244 363 7.35e-29 116
MS.gene042769.t1 AT1G79460 25.077 323 228 10 69 382 456 773 1.20e-21 97.4
MS.gene042769.t1 AT1G61120 20.942 382 286 5 1 371 388 764 4.58e-15 77.4

Find 67 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCCTTGACCCCTTCTTAA+AGG 0.174209 6.1:+61222662 MS.gene042769:CDS
TTTCCTTGCTTGAGAATATT+GGG 0.241792 6.1:-61222161 None:intergenic
ATGCAAGTAGAATTTCATTA+TGG 0.248221 6.1:-61221877 None:intergenic
GTCATCAATGTATGATAAAA+TGG 0.272756 6.1:-61222203 None:intergenic
CTGCTATACATTGCAGGCTT+AGG 0.276774 6.1:+61223631 MS.gene042769:CDS
AGCCTGCATCAAAAGGAATT+TGG 0.290423 6.1:+61221929 MS.gene042769:CDS
TAATTGCTTGGAGGATCTTC+CGG 0.335446 6.1:+61222557 MS.gene042769:CDS
TGAGGGAATATGATATGAAT+AGG 0.340079 6.1:+61223833 MS.gene042769:CDS
GAGTAGTAGAATGTTGCTTT+TGG 0.347068 6.1:+61222116 MS.gene042769:CDS
ATGCTGCTGCTATACATTGC+AGG 0.354080 6.1:+61223625 MS.gene042769:CDS
AGGACATGTTTCTTCGTACT+TGG 0.361077 6.1:+61223801 MS.gene042769:CDS
AACTCTTACTTCCTTTAAGA+AGG 0.385301 6.1:-61222673 None:intergenic
TGCAAGTAGAATTTCATTAT+GGG 0.387262 6.1:-61221876 None:intergenic
TCAGTTGGCTTAAGACATTC+CGG 0.387442 6.1:-61223913 None:intergenic
CGTACTTGGAATGTTATATG+AGG 0.388936 6.1:+61223815 MS.gene042769:CDS
GGATGAATCTTGCTCATAGA+TGG 0.390050 6.1:-61221855 None:intergenic
CGACAAAAGACATCTTTGAT+TGG 0.390521 6.1:+61223577 MS.gene042769:CDS
ATAGTTAGCATTCAGCCATT+TGG 0.402662 6.1:-61223460 None:intergenic
GTTGCTTTATAAATCACTCT+TGG 0.404999 6.1:+61222590 MS.gene042769:CDS
TGCATGTACTCCTCTGTTGT+TGG 0.418606 6.1:-61223486 None:intergenic
TAAAGCAACTTCATGTAATC+CGG 0.421098 6.1:-61222576 None:intergenic
GACAAAAGACATCTTTGATT+GGG 0.442878 6.1:+61223578 MS.gene042769:CDS
AAAAGAAAATTGACAACGCT+TGG 0.444020 6.1:+61223878 MS.gene042769:CDS
TGCTATACATTGCAGGCTTA+GGG 0.448464 6.1:+61223632 MS.gene042769:CDS
TAGGTGGGATATTAATTGCT+TGG 0.452782 6.1:+61222545 MS.gene042769:intron
GATGACACTTACGATGCATA+TGG 0.455602 6.1:+61222220 MS.gene042769:CDS
TAATGAAATTCTACTTGCAT+TGG 0.460272 6.1:+61221880 MS.gene042769:CDS
ACGACTTGTTACATTGGAAT+GGG 0.461126 6.1:+61223546 MS.gene042769:CDS
GCTCCAAAGCCTGCATCAAA+AGG 0.461740 6.1:+61221922 MS.gene042769:CDS
TACGACTTGTTACATTGGAA+TGG 0.469495 6.1:+61223545 MS.gene042769:CDS
GAGCAAGAAATGAAGAAAGA+AGG 0.470516 6.1:+61222630 MS.gene042769:CDS
CTTATATGAGTGAGGCCAAA+TGG 0.471857 6.1:+61223445 MS.gene042769:CDS
GAGGGAATATGATATGAATA+GGG 0.475500 6.1:+61223834 MS.gene042769:CDS
ACTCTTACTTCCTTTAAGAA+GGG 0.487114 6.1:-61222672 None:intergenic
TGGCCTCACTCATATAAGCT+TGG 0.493836 6.1:-61223440 None:intergenic
ATATTGGGGCTCAAAATATA+CGG 0.495144 6.1:-61222146 None:intergenic
CTTCCTTTAAGAAGGGGTCA+AGG 0.498434 6.1:-61222665 None:intergenic
TTGAGAACACACGTCAGAAT+AGG 0.502783 6.1:-61223946 None:intergenic
ATACAGGTGGTGGAAGGAGT+TGG 0.512387 6.1:+61222060 MS.gene042769:intron
CGCTTGGAAAGATATAAATC+CGG 0.516760 6.1:+61223894 MS.gene042769:CDS
TAACTTCTTGCCACTAGCCT+AGG 0.521919 6.1:-61221827 None:intergenic
GCCTCACATATGATGGTTCA+TGG 0.522278 6.1:+61221677 None:intergenic
GTGGGATATTAATTGCTTGG+AGG 0.529634 6.1:+61222548 MS.gene042769:CDS
AGGACTCAATCGTGTGGGCA+TGG 0.529918 6.1:-61221750 None:intergenic
TTCCTTGCTTGAGAATATTG+GGG 0.534381 6.1:-61222160 None:intergenic
ATAGGTAACGGAACTTCAGT+TGG 0.538099 6.1:-61223928 None:intergenic
AAACAGTTTGAGCAGAAAAG+AGG 0.539017 6.1:+61223781 MS.gene042769:intron
CTCTTACTTCCTTTAAGAAG+GGG 0.539589 6.1:-61222671 None:intergenic
TAACAAGTCGTAGCCAACAC+TGG 0.539809 6.1:-61223534 None:intergenic
TTCCTTTAAGAAGGGGTCAA+GGG 0.543212 6.1:-61222664 None:intergenic
AGCCCCAATATTCTCAAGCA+AGG 0.547455 6.1:+61222158 MS.gene042769:CDS
TTTACTTGTGTTGCAAGAGA+AGG 0.558925 6.1:-61221770 None:intergenic
ATCGTGTGGGCATGGACTCA+AGG 0.561173 6.1:-61221742 None:intergenic
GTACTTGGAATGTTATATGA+GGG 0.567173 6.1:+61223816 MS.gene042769:CDS
ATCAAGTGGTTACCCAGTGT+TGG 0.570889 6.1:+61223521 MS.gene042769:CDS
CTTTATATACAGGTGGTGGA+AGG 0.574461 6.1:+61222054 MS.gene042769:intron
AACAAGTCGTAGCCAACACT+GGG 0.585209 6.1:-61223533 None:intergenic
ACTACTCTATCTCGTGCATA+CGG 0.590301 6.1:-61222100 None:intergenic
CACATATCAACAATATCAAG+TGG 0.594145 6.1:+61223507 MS.gene042769:CDS
TTGGCTACGACTTGTTACAT+TGG 0.603074 6.1:+61223540 MS.gene042769:CDS
GATAACTACACACACTCTGA+CGG 0.610210 6.1:+61224006 MS.gene042769:CDS
AAGAGAAGGACTCAATCGTG+TGG 0.632456 6.1:-61221756 None:intergenic
AGAGAAGGACTCAATCGTGT+GGG 0.635217 6.1:-61221755 None:intergenic
AATCCAAGCTTATATGAGTG+AGG 0.644710 6.1:+61223437 MS.gene042769:CDS
GGATGAAATTGTGTCAAGCG+AGG 0.666273 6.1:+61223653 MS.gene042769:CDS
ACACACGTCAGAATAGGTAA+CGG 0.688917 6.1:-61223940 None:intergenic
TAACTATATACCAACAACAG+AGG 0.703519 6.1:+61223476 MS.gene042769:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCATTTTAAATTTTTTG+TGG + chr6.1:61222789-61222808 MS.gene042769:intron 10.0%
!!! CAATTAACATTTATTTTATA+TGG + chr6.1:61223389-61223408 MS.gene042769:intron 10.0%
!! TATAAAGACAAAAATAGTAT+AGG - chr6.1:61222041-61222060 None:intergenic 15.0%
!!! AAACATTTTTACATCATTTT+AGG + chr6.1:61222890-61222909 MS.gene042769:intron 15.0%
!!! TATTGTTTATCTTTAAAGTT+TGG - chr6.1:61222335-61222354 None:intergenic 15.0%
!!! TTAATTACATAGTTTTTCTT+TGG + chr6.1:61223362-61223381 MS.gene042769:intron 15.0%
!!! TTGAAATCAAAATGATTTTT+AGG + chr6.1:61222526-61222545 MS.gene042769:intron 15.0%
!!! TTGCATTAATTTTTATTCTT+TGG - chr6.1:61223169-61223188 None:intergenic 15.0%
!! TGTAAAAAACTTATTATCCA+TGG + chr6.1:61223028-61223047 MS.gene042769:intron 20.0%
!!! AAATCAAAATGATTTTTAGG+TGG + chr6.1:61222529-61222548 MS.gene042769:intron 20.0%
!!! AATCAAAATGATTTTTAGGT+GGG + chr6.1:61222530-61222549 MS.gene042769:intron 20.0%
!!! AATTTTTTGTGGTTATCAAA+TGG + chr6.1:61222800-61222819 MS.gene042769:intron 20.0%
!!! CTATTTTTGTCTTTATATAC+AGG + chr6.1:61222044-61222063 MS.gene042769:intron 20.0%
!!! TATCTTGACATTTTAATTAC+TGG + chr6.1:61223296-61223315 MS.gene042769:intron 20.0%
!!! TTCTTTTAACAATAGAGTAA+TGG + chr6.1:61222497-61222516 MS.gene042769:intron 20.0%
! AACTCTGTTAATAAAATCCA+TGG - chr6.1:61223048-61223067 None:intergenic 25.0%
! AAGAAAAATGTATCATCATG+AGG + chr6.1:61223105-61223124 MS.gene042769:intron 25.0%
! AATTTATCCATCTTCAATCT+TGG + chr6.1:61222409-61222428 MS.gene042769:intron 25.0%
! ATGCAAGTAGAATTTCATTA+TGG - chr6.1:61221880-61221899 None:intergenic 25.0%
! GTCATCAATGTATGATAAAA+TGG - chr6.1:61222206-61222225 None:intergenic 25.0%
! TAATGAAATTCTACTTGCAT+TGG + chr6.1:61221880-61221899 MS.gene042769:CDS 25.0%
! TGCAAGTAGAATTTCATTAT+GGG - chr6.1:61221879-61221898 None:intergenic 25.0%
! TTCAATCAAATAACTTCATG+AGG - chr6.1:61222966-61222985 None:intergenic 25.0%
!! TCTTTAATCTAGTTTAGAGT+TGG + chr6.1:61222364-61222383 MS.gene042769:intron 25.0%
!! TTTTTGTCTTTATATACAGG+TGG + chr6.1:61222047-61222066 MS.gene042769:intron 25.0%
!!! TGAATTAGAACTTTTGACTA+AGG + chr6.1:61222252-61222271 MS.gene042769:CDS 25.0%
AAAAATATTGGAAGAGGCTT+TGG + chr6.1:61221703-61221722 MS.gene042769:CDS 30.0%
AAAAGAAAATTGACAACGCT+TGG + chr6.1:61223878-61223897 MS.gene042769:CDS 30.0%
AAACTGAGTTCATATAACCA+TGG - chr6.1:61223204-61223223 None:intergenic 30.0%
ATATTGGGGCTCAAAATATA+CGG - chr6.1:61222149-61222168 None:intergenic 30.0%
CACATATCAACAATATCAAG+TGG + chr6.1:61223507-61223526 MS.gene042769:CDS 30.0%
GACAAAAGACATCTTTGATT+GGG + chr6.1:61223578-61223597 MS.gene042769:CDS 30.0%
GAGGGAATATGATATGAATA+GGG + chr6.1:61223834-61223853 MS.gene042769:CDS 30.0%
GGTTCATGGTGAAAAAATAT+TGG + chr6.1:61221691-61221710 MS.gene042769:CDS 30.0%
GTACTTGGAATGTTATATGA+GGG + chr6.1:61223816-61223835 MS.gene042769:CDS 30.0%
TAAAGCAACTTCATGTAATC+CGG - chr6.1:61222579-61222598 None:intergenic 30.0%
TAACTATATACCAACAACAG+AGG + chr6.1:61223476-61223495 MS.gene042769:CDS 30.0%
TGAGGGAATATGATATGAAT+AGG + chr6.1:61223833-61223852 MS.gene042769:CDS 30.0%
TGCAAAAAACTTATTGTCCA+TGG + chr6.1:61223184-61223203 MS.gene042769:intron 30.0%
TGGTGAAAAAATATTGGAAG+AGG + chr6.1:61221697-61221716 MS.gene042769:CDS 30.0%
TGGTTATCAAATGGAAGTTT+AGG + chr6.1:61222809-61222828 MS.gene042769:intron 30.0%
TTTCCTTGCTTGAGAATATT+GGG - chr6.1:61222164-61222183 None:intergenic 30.0%
! AAAGTATCACCATAATCACT+TGG + chr6.1:61222925-61222944 MS.gene042769:intron 30.0%
! AACTCTTACTTCCTTTAAGA+AGG - chr6.1:61222676-61222695 None:intergenic 30.0%
! ACTCTTACTTCCTTTAAGAA+GGG - chr6.1:61222675-61222694 None:intergenic 30.0%
! GTTGCTTTATAAATCACTCT+TGG + chr6.1:61222590-61222609 MS.gene042769:CDS 30.0%
! TCATGAGGATACATTTTTCT+TGG - chr6.1:61222951-61222970 None:intergenic 30.0%
! TTTCTTTTGGCATTCTTGAA+TGG - chr6.1:61223866-61223885 None:intergenic 30.0%
! TTTTCCTTGCTTGAGAATAT+TGG - chr6.1:61222165-61222184 None:intergenic 30.0%
!! AAGCGTTGTCAATTTTCTTT+TGG - chr6.1:61223879-61223898 None:intergenic 30.0%
!! TTTTGACTAAGGCAATTGAA+AGG + chr6.1:61222263-61222282 MS.gene042769:CDS 30.0%
!!! AGTAGTAGAATGTTGCTTTT+GGG + chr6.1:61222117-61222136 MS.gene042769:CDS 30.0%
AAACAGTTTGAGCAGAAAAG+AGG + chr6.1:61223781-61223800 MS.gene042769:intron 35.0%
AATCCAAGCTTATATGAGTG+AGG + chr6.1:61223437-61223456 MS.gene042769:CDS 35.0%
ATAGTTAGCATTCAGCCATT+TGG - chr6.1:61223463-61223482 None:intergenic 35.0%
CGACAAAAGACATCTTTGAT+TGG + chr6.1:61223577-61223596 MS.gene042769:CDS 35.0%
CGCTTGGAAAGATATAAATC+CGG + chr6.1:61223894-61223913 MS.gene042769:CDS 35.0%
CGTACTTGGAATGTTATATG+AGG + chr6.1:61223815-61223834 MS.gene042769:CDS 35.0%
GAGCAAGAAATGAAGAAAGA+AGG + chr6.1:61222630-61222649 MS.gene042769:CDS 35.0%
TAGGTGGGATATTAATTGCT+TGG + chr6.1:61222545-61222564 MS.gene042769:intron 35.0%
TTCCTTGCTTGAGAATATTG+GGG - chr6.1:61222163-61222182 None:intergenic 35.0%
TTGTCTTTATATACAGGTGG+TGG + chr6.1:61222050-61222069 MS.gene042769:intron 35.0%
TTTACTTGTGTTGCAAGAGA+AGG - chr6.1:61221773-61221792 None:intergenic 35.0%
! CTCTTACTTCCTTTAAGAAG+GGG - chr6.1:61222674-61222693 None:intergenic 35.0%
! GCAGCAGCATTAACAATTTT+TGG - chr6.1:61223615-61223634 None:intergenic 35.0%
! TTCCAAATTCCTTTTGATGC+AGG - chr6.1:61221934-61221953 None:intergenic 35.0%
! TTTTCACCATGAACCATCAT+AGG - chr6.1:61221686-61221705 None:intergenic 35.0%
! TTTTCTTGGCCAAGTGATTA+TGG - chr6.1:61222937-61222956 None:intergenic 35.0%
!! ACGACTTGTTACATTGGAAT+GGG + chr6.1:61223546-61223565 MS.gene042769:CDS 35.0%
!! TACGACTTGTTACATTGGAA+TGG + chr6.1:61223545-61223564 MS.gene042769:CDS 35.0%
!!! GAGTAGTAGAATGTTGCTTT+TGG + chr6.1:61222116-61222135 MS.gene042769:CDS 35.0%
ACACACGTCAGAATAGGTAA+CGG - chr6.1:61223943-61223962 None:intergenic 40.0%
ACTACTCTATCTCGTGCATA+CGG - chr6.1:61222103-61222122 None:intergenic 40.0%
AGCCTGCATCAAAAGGAATT+TGG + chr6.1:61221929-61221948 MS.gene042769:CDS 40.0%
AGGACATGTTTCTTCGTACT+TGG + chr6.1:61223801-61223820 MS.gene042769:CDS 40.0%
CTTATATGAGTGAGGCCAAA+TGG + chr6.1:61223445-61223464 MS.gene042769:CDS 40.0%
GATAACTACACACACTCTGA+CGG + chr6.1:61224006-61224025 MS.gene042769:CDS 40.0%
GATGACACTTACGATGCATA+TGG + chr6.1:61222220-61222239 MS.gene042769:CDS 40.0%
GGATGAATCTTGCTCATAGA+TGG - chr6.1:61221858-61221877 None:intergenic 40.0%
GTGGGATATTAATTGCTTGG+AGG + chr6.1:61222548-61222567 MS.gene042769:CDS 40.0%
TAATTGCTTGGAGGATCTTC+CGG + chr6.1:61222557-61222576 MS.gene042769:CDS 40.0%
TGCTATACATTGCAGGCTTA+GGG + chr6.1:61223632-61223651 MS.gene042769:CDS 40.0%
TTAGCGACCAAGATTGAAGA+TGG - chr6.1:61222419-61222438 None:intergenic 40.0%
TTGAGAACACACGTCAGAAT+AGG - chr6.1:61223949-61223968 None:intergenic 40.0%
! ATTCCTTTTGATGCAGGCTT+TGG - chr6.1:61221928-61221947 None:intergenic 40.0%
! TCAGTTGGCTTAAGACATTC+CGG - chr6.1:61223916-61223935 None:intergenic 40.0%
! TTCCTTTAAGAAGGGGTCAA+GGG - chr6.1:61222667-61222686 None:intergenic 40.0%
!! ATAGGTAACGGAACTTCAGT+TGG - chr6.1:61223931-61223950 None:intergenic 40.0%
!! CAATCTCTCGCGTTTTATTG+AGG + chr6.1:61223966-61223985 MS.gene042769:CDS 40.0%
!! CTTTATATACAGGTGGTGGA+AGG + chr6.1:61222054-61222073 MS.gene042769:intron 40.0%
!! TTGGCTACGACTTGTTACAT+TGG + chr6.1:61223540-61223559 MS.gene042769:CDS 40.0%
AACAAGTCGTAGCCAACACT+GGG - chr6.1:61223536-61223555 None:intergenic 45.0%
AAGAGAAGGACTCAATCGTG+TGG - chr6.1:61221759-61221778 None:intergenic 45.0%
AGAGAAGGACTCAATCGTGT+GGG - chr6.1:61221758-61221777 None:intergenic 45.0%
AGCCCCAATATTCTCAAGCA+AGG + chr6.1:61222158-61222177 MS.gene042769:CDS 45.0%
ATCAAGTGGTTACCCAGTGT+TGG + chr6.1:61223521-61223540 MS.gene042769:CDS 45.0%
ATGCTGCTGCTATACATTGC+AGG + chr6.1:61223625-61223644 MS.gene042769:CDS 45.0%
CAAAAACTTGCCTAGGCTAG+TGG + chr6.1:61221817-61221836 MS.gene042769:CDS 45.0%
CTGCTATACATTGCAGGCTT+AGG + chr6.1:61223631-61223650 MS.gene042769:CDS 45.0%
GGATGAAATTGTGTCAAGCG+AGG + chr6.1:61223653-61223672 MS.gene042769:CDS 45.0%
TAACAAGTCGTAGCCAACAC+TGG - chr6.1:61223537-61223556 None:intergenic 45.0%
TGCATGTACTCCTCTGTTGT+TGG - chr6.1:61223489-61223508 None:intergenic 45.0%
TGGCCTCACTCATATAAGCT+TGG - chr6.1:61223443-61223462 None:intergenic 45.0%
! CTAGCCTAGGCAAGTTTTTG+CGG - chr6.1:61221817-61221836 None:intergenic 45.0%
! CTTCCTTTAAGAAGGGGTCA+AGG - chr6.1:61222668-61222687 None:intergenic 45.0%
! TAACTTCTTGCCACTAGCCT+AGG - chr6.1:61221830-61221849 None:intergenic 45.0%
!!! GAGGCTTTGGCTTTTACTTC+TGG + chr6.1:61221716-61221735 MS.gene042769:CDS 45.0%
CACCCTTGACCCCTTCTTAA+AGG + chr6.1:61222662-61222681 MS.gene042769:CDS 50.0%
CTCTCCGCAAAAACTTGCCT+AGG + chr6.1:61221810-61221829 MS.gene042769:CDS 50.0%
GCTCCAAAGCCTGCATCAAA+AGG + chr6.1:61221922-61221941 MS.gene042769:CDS 50.0%
!! ATACAGGTGGTGGAAGGAGT+TGG + chr6.1:61222060-61222079 MS.gene042769:intron 50.0%
AGGACTCAATCGTGTGGGCA+TGG - chr6.1:61221753-61221772 None:intergenic 55.0%
ATCGTGTGGGCATGGACTCA+AGG - chr6.1:61221745-61221764 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 61221686 61224068 61221686 ID=MS.gene042769
chr6.1 mRNA 61221686 61224068 61221686 ID=MS.gene042769.t1;Parent=MS.gene042769
chr6.1 exon 61221686 61221963 61221686 ID=MS.gene042769.t1.exon1;Parent=MS.gene042769.t1
chr6.1 CDS 61221686 61221963 61221686 ID=cds.MS.gene042769.t1;Parent=MS.gene042769.t1
chr6.1 exon 61222066 61222284 61222066 ID=MS.gene042769.t1.exon2;Parent=MS.gene042769.t1
chr6.1 CDS 61222066 61222284 61222066 ID=cds.MS.gene042769.t1;Parent=MS.gene042769.t1
chr6.1 exon 61222548 61222686 61222548 ID=MS.gene042769.t1.exon3;Parent=MS.gene042769.t1
chr6.1 CDS 61222548 61222686 61222548 ID=cds.MS.gene042769.t1;Parent=MS.gene042769.t1
chr6.1 exon 61223426 61223674 61223426 ID=MS.gene042769.t1.exon4;Parent=MS.gene042769.t1
chr6.1 CDS 61223426 61223674 61223426 ID=cds.MS.gene042769.t1;Parent=MS.gene042769.t1
chr6.1 exon 61223787 61224068 61223787 ID=MS.gene042769.t1.exon5;Parent=MS.gene042769.t1
chr6.1 CDS 61223787 61224068 61223787 ID=cds.MS.gene042769.t1;Parent=MS.gene042769.t1
Gene Sequence

>MS.gene042769

ATGATGGTTCATGGTGAAAAAATATTGGAAGAGGCTTTGGCTTTTACTTCTGGTCACCTTGAGTCCATGCCCACACGATTGAGTCCTTCTCTTGCAACACAAGTAAAACAAAGCTTAAGACAATCTCTCCGCAAAAACTTGCCTAGGCTAGTGGCAAGAAGTTACATTTCCATCTATGAGCAAGATTCATCCCATAATGAAATTCTACTTGCATTGGCAAAATTAGATTTTAATATGCTCCAAAGCCTGCATCAAAAGGAATTTGGAAACATTAGCAAGTAAGTACATCTTGAGCATACATCAAAATACAAAAGTATATTAAATTGAGTTCAAAAAATAATTATACTAATTTCCTATACTATTTTTGTCTTTATATACAGGTGGTGGAAGGAGTTGGATATTGCTCAAAAACTACCGTATGCACGAGATAGAGTAGTAGAATGTTGCTTTTGGGTTATGACCGTATATTTTGAGCCCCAATATTCTCAAGCAAGGAAAATGATGTCAAAATTTATCTCCATTTTATCATACATTGATGACACTTACGATGCATATGGAACTATTGATGAATTAGAACTTTTGACTAAGGCAATTGAAAGGTTAGCACATCATACAATATAATTGTTAAGAATAATTGAATTCGTGACCAAACTTTAAAGATAAACAATATGAAGTTTATCTTTAATCTAGTTTAGAGTTGGAATTTTTTTTAATAATAACTTCAATTTATCCATCTTCAATCTTGGTCGCTAATTCAATTTTTTACAAGAAATATAGACATTTTCTTCACAAGTTTTTGTACGCGATATGTTTCTTTTAACAATAGAGTAATGGTTAATATTGAAATCAAAATGATTTTTAGGTGGGATATTAATTGCTTGGAGGATCTTCCGGATTACATGAAGTTGCTTTATAAATCACTCTTGGATGTTTATGAAGAGATAGAGCAAGAAATGAAGAAAGAAGGAAGAGAATACACCCTTGACCCCTTCTTAAAGGAAGTAAGAGTTTTGCTATATTGTTAGTATTATTAGTAATAATAAAGCATTAGTTAAAATTTGTTTCTTTTAGTGATTTATAATTTATTCAACAATTGAACAAAAATTTCATTTTAAATTTTTTGTGGTTATCAAATGGAAGTTTAGGATTTTATTTGCACATACTATCAATATAAATACATATATCATATCAAATCTCAACTTTAAAACATTTTTACATCATTTTAGGAATTAACATGATAAAGTATCACCATAATCACTTGGCCAAGAAAAATGTATCCTCATGAAGTTATTTGATTGAATGTAATGTTACAAACTTTTCTACTATCAATAATAAAAATTAATGTAAAAAACTTATTATCCATGGATTTTATTAACAGAGTTCATTAACATGATAAAGTATCATCATGATGACTTGACAAAGAAAAATGTATCATCATGAGGTTATTTGATTGAATGCAATGTTAAACAAAATTCTACTACCAAAGAATAAAAATTAATGCAAAAAACTTATTGTCCATGGTTATATGAACTCAGTTTGTAGTAATAACGATAATAAATTTTGTTTTGTCGTAAAAGAGCTCATTTGTAATATTAATTCTACGAAAGATATATCTTGACATTTTAATTACTGGTTAGATGAAAACTAAAAAAAATTGCTAATACAATTTAACATCATTAATTACATAGTTTTTCTTTGGTACACAATTAACATTTATTTTATATGGTATACTAATGACAGTTCAAAAATATAATCCAAGCTTATATGAGTGAGGCCAAATGGCTGAATGCTAACTATATACCAACAACAGAGGAGTACATGCACATATCAACAATATCAAGTGGTTACCCAGTGTTGGCTACGACTTGTTACATTGGAATGGGAAACATAGCGACAAAAGACATCTTTGATTGGGTAACTAACAATCCAAAAATTGTTAATGCTGCTGCTATACATTGCAGGCTTAGGGATGAAATTGTGTCAAGCGAGGTAATTTTTGAAAAGCGTTTAAAAAATTAATATAATAAGTTGACATAATGAAGAAATGTGACTCTTGATATATCTAATTTATCTAATATGATTTATATACTCTGATAAACAGTTTGAGCAGAAAAGAGGACATGTTTCTTCGTACTTGGAATGTTATATGAGGGAATATGATATGAATAGGGAAGCTGCCATTCAAGAATGCCAAAAGAAAATTGACAACGCTTGGAAAGATATAAATCCGGAATGTCTTAAGCCAACTGAAGTTCCGTTACCTATTCTGACGTGTGTTCTCAATCTCTCGCGTTTTATTGAGGTTTTTTACAAAGATACAGATAACTACACACACTCTGACGGACTAATGAAGACATATATTAATGATTTGCTTGTCAATCCT
Protein sequence

>MS.gene042769.t1

MMVHGEKILEEALAFTSGHLESMPTRLSPSLATQVKQSLRQSLRKNLPRLVARSYISIYEQDSSHNEILLALAKLDFNMLQSLHQKEFGNISKWWKELDIAQKLPYARDRVVECCFWVMTVYFEPQYSQARKMMSKFISILSYIDDTYDAYGTIDELELLTKAIERWDINCLEDLPDYMKLLYKSLLDVYEEIEQEMKKEGREYTLDPFLKEFKNIIQAYMSEAKWLNANYIPTTEEYMHISTISSGYPVLATTCYIGMGNIATKDIFDWVTNNPKIVNAAAIHCRLRDEIVSSEFEQKRGHVSSYLECYMREYDMNREAAIQECQKKIDNAWKDINPECLKPTEVPLPILTCVLNLSRFIEVFYKDTDNYTHSDGLMKTYINDLLVNP