Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04307.t1 | XP_024635441.1 | 94.8 | 688 | 34 | 2 | 1 | 687 | 1 | 687 | 0.00E+00 | 1275 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04307.t1 | Q9LG26 | 58.9 | 637 | 253 | 2 | 22 | 658 | 21 | 648 | 9.1e-215 | 748.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04307.t1 | A0A396ISR3 | 94.8 | 688 | 34 | 2 | 1 | 687 | 1 | 687 | 0.0e+00 | 1275.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene043239 | MS.gene04307 | PPI |
| MS.gene04307 | MS.gene21710 | PPI |
| MS.gene21912 | MS.gene04307 | PPI |
| MS.gene79599 | MS.gene04307 | PPI |
| MS.gene04307 | MS.gene024734 | PPI |
| MS.gene91519 | MS.gene04307 | PPI |
| MS.gene04307 | MS.gene021698 | PPI |
| MS.gene04307 | MS.gene058147 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04307.t1 | MTR_3g460830 | 97.925 | 241 | 5 | 0 | 447 | 687 | 5 | 245 | 2.11e-175 | 500 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04307.t1 | AT1G55870 | 59.486 | 622 | 243 | 2 | 37 | 658 | 36 | 648 | 0.0 | 755 |
| MS.gene04307.t1 | AT1G55870 | 55.603 | 464 | 199 | 1 | 195 | 658 | 8 | 464 | 1.93e-179 | 520 |
| MS.gene04307.t1 | AT1G55870 | 60.580 | 345 | 129 | 1 | 314 | 658 | 1 | 338 | 5.60e-145 | 427 |
| MS.gene04307.t1 | AT1G55870 | 60.580 | 345 | 129 | 1 | 314 | 658 | 1 | 338 | 5.60e-145 | 427 |
| MS.gene04307.t1 | AT3G25430 | 27.516 | 636 | 376 | 19 | 18 | 635 | 4 | 572 | 2.60e-60 | 213 |
| MS.gene04307.t1 | AT3G25430 | 27.358 | 636 | 360 | 20 | 18 | 635 | 4 | 555 | 1.83e-57 | 205 |
Find 137 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTCAGGAGCTTTCTGGTTT+TGG | 0.164511 | 3.4:-56044899 | MS.gene04307:CDS |
| TTTCCACGTCAGGAGCTTTC+TGG | 0.182890 | 3.4:-56044905 | MS.gene04307:CDS |
| TTCCTTGTATTCAATCAATT+TGG | 0.228163 | 3.4:+56039352 | None:intergenic |
| TTGAGAACATTGTTATTATC+TGG | 0.233378 | 3.4:-56039706 | MS.gene04307:CDS |
| CTCCTAAATCACTGCATAAC+TGG | 0.233696 | 3.4:+56039909 | None:intergenic |
| GCCCAGTTATGCAGTGATTT+AGG | 0.257302 | 3.4:-56039911 | MS.gene04307:CDS |
| TGCTTCCCTTTCTTGTTCTC+TGG | 0.265913 | 3.4:+56044385 | None:intergenic |
| GACTACTTCGATGGATTTCT+TGG | 0.268029 | 3.4:-56044852 | MS.gene04307:CDS |
| TTTCTAAATTAAATGATGTT+AGG | 0.268163 | 3.4:-56044319 | MS.gene04307:CDS |
| TGTAACTAGCTCGGTGCTTT+TGG | 0.289237 | 3.4:+56043053 | None:intergenic |
| CTCGTACTTCACTTGCCTTC+AGG | 0.295775 | 3.4:+56039669 | None:intergenic |
| GCATCCGCTGGGAAAACTTT+TGG | 0.305585 | 3.4:-56039489 | MS.gene04307:CDS |
| AGAAACTCTGTTTGCTGATC+AGG | 0.312532 | 3.4:-56039402 | MS.gene04307:CDS |
| TCACTTGCCTTCAGGTTAGA+AGG | 0.335224 | 3.4:+56039677 | None:intergenic |
| TTTACATTCTCTTCTATAGT+TGG | 0.344960 | 3.4:+56039302 | None:intergenic |
| CATCCAGCTCTCAAGCTTGA+TGG | 0.345888 | 3.4:-56044131 | MS.gene04307:CDS |
| AATGTGAACAATGAGGTTTC+TGG | 0.349688 | 3.4:-56043860 | MS.gene04307:CDS |
| AACAAGAAAGGGAAGCAATA+AGG | 0.360923 | 3.4:-56044379 | MS.gene04307:CDS |
| TTGTGGCGGTTGAAGAGGTT+AGG | 0.365222 | 3.4:+56046362 | None:intergenic |
| AGTTTGATCGCTCCGATGTT+CGG | 0.382519 | 3.4:-56046171 | MS.gene04307:CDS |
| CATGTCTACAGCAAATTTAT+CGG | 0.389497 | 3.4:-56043252 | MS.gene04307:CDS |
| CTGTATACACAACTATTTGT+TGG | 0.390531 | 3.4:+56043834 | None:intergenic |
| TCATTTAATTTAGAAATGTC+TGG | 0.392693 | 3.4:+56044326 | None:intergenic |
| AAGTTGAAGTTGATGATTCA+AGG | 0.401865 | 3.4:-56043011 | MS.gene04307:intron |
| TTCTAAATTAAATGATGTTA+GGG | 0.404843 | 3.4:-56044318 | MS.gene04307:CDS |
| ATGGCGTGATGGGTTATTAC+AGG | 0.412013 | 3.4:-56044231 | MS.gene04307:CDS |
| GGATGAACTAAACTTACTTC+TGG | 0.413972 | 3.4:-56043804 | MS.gene04307:intron |
| TGTTCTCAAACAAAATTTGT+TGG | 0.415338 | 3.4:+56039720 | None:intergenic |
| TGAGAACATTGTTATTATCT+GGG | 0.418628 | 3.4:-56039705 | MS.gene04307:CDS |
| AGCGGATGCAAAACCGAGAT+TGG | 0.425664 | 3.4:+56039503 | None:intergenic |
| GATGCTTATAAAGAAATTTG+TGG | 0.441063 | 3.4:-56039437 | MS.gene04307:CDS |
| GAACATCGGAGCGATCAAAC+TGG | 0.442573 | 3.4:+56046173 | None:intergenic |
| GGTTATTACAGGAGCAAAAT+CGG | 0.443983 | 3.4:-56044220 | MS.gene04307:CDS |
| TACATGCATTAAAATCAAAC+TGG | 0.446942 | 3.4:+56044822 | None:intergenic |
| TGTATACACAACTATTTGTT+GGG | 0.447606 | 3.4:+56043835 | None:intergenic |
| TCGGCGTTTGTCCTTTCCGA+TGG | 0.449616 | 3.4:-56046102 | MS.gene04307:CDS |
| AGGTTCGCCGTTGTTCAGTT+CGG | 0.453497 | 3.4:-56046121 | MS.gene04307:CDS |
| TGTCTGGACACTCACTATCA+TGG | 0.458158 | 3.4:+56044342 | None:intergenic |
| TTGGAGCCAGCAGCAATTTG+TGG | 0.460161 | 3.4:+56043072 | None:intergenic |
| ATTTATCCAGAGAACAAGAA+AGG | 0.462100 | 3.4:-56044391 | MS.gene04307:CDS |
| TGTGGCGGTTGAAGAGGTTA+GGG | 0.473888 | 3.4:+56046363 | None:intergenic |
| CCTGCCTCATGCTTGCCTCC+AGG | 0.474138 | 3.4:+56039965 | None:intergenic |
| AGAAATCCATCGAAGTAGTC+TGG | 0.482313 | 3.4:+56044855 | None:intergenic |
| AAACTTCGAATCGTCGTTAG+CGG | 0.483892 | 3.4:-56046284 | MS.gene04307:CDS |
| TGTAGTTTCTGTCTACCACT+TGG | 0.491252 | 3.4:-56039615 | MS.gene04307:CDS |
| GCAAACAGAGTTTCTGATAA+GGG | 0.491406 | 3.4:+56039410 | None:intergenic |
| CGGCGTTTGTCCTTTCCGAT+GGG | 0.493200 | 3.4:-56046101 | MS.gene04307:CDS |
| CTTGTAGAAGGAGGTAAAAG+AGG | 0.494009 | 3.4:-56043476 | MS.gene04307:intron |
| CTGAACAACGGCGAACCTAC+TGG | 0.497338 | 3.4:+56046126 | None:intergenic |
| TCAGAACAGAAGTTGATAGT+TGG | 0.502195 | 3.4:-56043382 | MS.gene04307:CDS |
| TATATCTTAGTTATATGCAT+GGG | 0.502273 | 3.4:-56039814 | MS.gene04307:CDS |
| AATCGTCGTTAGCGGAGCTC+CGG | 0.504255 | 3.4:-56046276 | MS.gene04307:CDS |
| GGATATGATGCTTTCATGAC+TGG | 0.504444 | 3.4:-56039944 | MS.gene04307:CDS |
| AGCAAACAGAGTTTCTGATA+AGG | 0.504846 | 3.4:+56039409 | None:intergenic |
| GAAAGCTTAGAGAGAAGTAA+TGG | 0.511591 | 3.4:-56039527 | MS.gene04307:CDS |
| TGGCAGAGAAATTCATTACA+TGG | 0.515487 | 3.4:+56044875 | None:intergenic |
| TCAGCTTAAGTTGATTCAGT+TGG | 0.517220 | 3.4:-56044096 | MS.gene04307:intron |
| ACGGATATGCCACGAAGGAC+TGG | 0.517851 | 3.4:+56046068 | None:intergenic |
| CCTGGAGGCAAGCATGAGGC+AGG | 0.518137 | 3.4:-56039965 | MS.gene04307:CDS |
| AACCAAATTGATTGAATACA+AGG | 0.519448 | 3.4:-56039354 | MS.gene04307:CDS |
| AGATCGTCCAGCTGGAATCC+TGG | 0.520249 | 3.4:-56039983 | MS.gene04307:CDS |
| AGGTAAAAGAGGAGAATCAA+AGG | 0.522105 | 3.4:-56043465 | MS.gene04307:CDS |
| GGCGAAATCCAACTGCAGCT+TGG | 0.522198 | 3.4:+56043425 | None:intergenic |
| TCCTAAATCACTGCATAACT+GGG | 0.523426 | 3.4:+56039910 | None:intergenic |
| TTTATCCAGAGAACAAGAAA+GGG | 0.523900 | 3.4:-56044390 | MS.gene04307:CDS |
| TGAAAGATAAGTTCAGTGAA+TGG | 0.529459 | 3.4:-56044250 | MS.gene04307:CDS |
| GAATTTGCAGATCGTCCAGC+TGG | 0.533683 | 3.4:-56039991 | MS.gene04307:intron |
| TTCGTCTAACCAGTCCTTCG+TGG | 0.534068 | 3.4:-56046077 | MS.gene04307:CDS |
| TTCGAAGTTTGCAACGGTGA+CGG | 0.534899 | 3.4:+56046297 | None:intergenic |
| GCTGATCAGGCAAAGGCAGT+TGG | 0.534922 | 3.4:-56039389 | MS.gene04307:CDS |
| GCAACGGTGACGGTCTTCGA+TGG | 0.535587 | 3.4:+56046307 | None:intergenic |
| AATCGGTCAGACCAAATTCA+AGG | 0.539111 | 3.4:-56044203 | MS.gene04307:CDS |
| AAGTTCAGTGAATGGCGTGA+TGG | 0.547765 | 3.4:-56044242 | MS.gene04307:CDS |
| CTATATCTTAGTTATATGCA+TGG | 0.549824 | 3.4:-56039815 | MS.gene04307:CDS |
| AGTTCAGTGAATGGCGTGAT+GGG | 0.550536 | 3.4:-56044241 | MS.gene04307:CDS |
| CCGACGATCGGACATGGTGC+CGG | 0.551309 | 3.4:+56046257 | None:intergenic |
| TTCCTTCAACAAAAGAAAGT+CGG | 0.553574 | 3.4:+56039547 | None:intergenic |
| AAACTGGAATGAATCACGCC+AGG | 0.554543 | 3.4:+56046189 | None:intergenic |
| CGGTGGTGTGGTTGTTGTGG+CGG | 0.555354 | 3.4:+56046348 | None:intergenic |
| GTCTGGACACTCACTATCAT+GGG | 0.555910 | 3.4:+56044343 | None:intergenic |
| GGTTGTTGTGGCGGTTGAAG+AGG | 0.557395 | 3.4:+56046357 | None:intergenic |
| GATATTATAGATCTAAATAC+CGG | 0.557851 | 3.4:-56039791 | MS.gene04307:CDS |
| GGGATTTCCTTCTAACCTGA+AGG | 0.558626 | 3.4:-56039684 | MS.gene04307:CDS |
| CTTGCCTCCAGGATTCCAGC+TGG | 0.558683 | 3.4:+56039976 | None:intergenic |
| CAAAGGCAGTTGGCATGAAG+TGG | 0.559051 | 3.4:-56039379 | MS.gene04307:CDS |
| TCAACTTCTGTTCTGATGAA+AGG | 0.559958 | 3.4:+56043389 | None:intergenic |
| GAGAAGTAATGGTCCAATCT+CGG | 0.560192 | 3.4:-56039516 | MS.gene04307:CDS |
| CGTGATAGTGTCGAAGAAGA+AGG | 0.563909 | 3.4:+56046400 | None:intergenic |
| ATGAAGATCCAAGCTGCAGT+TGG | 0.563917 | 3.4:-56043433 | MS.gene04307:CDS |
| AATCCATCAAGCTTGAGAGC+TGG | 0.565036 | 3.4:+56044128 | None:intergenic |
| GCCATCGACCTCGAGATGAC+CGG | 0.569325 | 3.4:-56046226 | MS.gene04307:CDS |
| TTGCGGTGGTGTGGTTGTTG+TGG | 0.569522 | 3.4:+56046345 | None:intergenic |
| TTTAATGCATGTATACATGA+AGG | 0.569703 | 3.4:-56044812 | MS.gene04307:intron |
| TCTCTGCCAGACTACTTCGA+TGG | 0.569944 | 3.4:-56044861 | MS.gene04307:CDS |
| CGAAGTCCGACGATCGGACA+TGG | 0.576467 | 3.4:+56046251 | None:intergenic |
| TTGGGATGGTGTAACTAGCT+CGG | 0.581065 | 3.4:+56043044 | None:intergenic |
| CGTCTCTTGAGGTTATAGCT+TGG | 0.582065 | 3.4:+56039746 | None:intergenic |
| TGGCGACGAAGTCCGACGAT+CGG | 0.583110 | 3.4:+56046245 | None:intergenic |
| GAGAACATTGTTATTATCTG+GGG | 0.588898 | 3.4:-56039704 | MS.gene04307:CDS |
| CTCCGATGTTCGGTATCTCA+AGG | 0.590111 | 3.4:-56046161 | MS.gene04307:CDS |
| GGTAAAAGAGGAGAATCAAA+GGG | 0.595963 | 3.4:-56043464 | MS.gene04307:CDS |
| AGGTTAGAGACTCCGCCAGT+AGG | 0.598496 | 3.4:-56046141 | MS.gene04307:CDS |
| TCCGGTCATCTCGAGGTCGA+TGG | 0.602825 | 3.4:+56046225 | None:intergenic |
| TCTGTTTGCTGATCAGGCAA+AGG | 0.603663 | 3.4:-56039396 | MS.gene04307:CDS |
| ATGTCTGCCATGTTGACAGA+AGG | 0.603946 | 3.4:+56044290 | None:intergenic |
| TTATATCAATGTGAACAATG+AGG | 0.605285 | 3.4:-56043867 | MS.gene04307:CDS |
| TTGTAACTCCGGTCATCTCG+AGG | 0.608427 | 3.4:+56046218 | None:intergenic |
| AAACCAGAAAGCTCCTGACG+TGG | 0.613387 | 3.4:+56044902 | None:intergenic |
| CCGGAGTTACAAGTTCTCCC+TGG | 0.615817 | 3.4:-56046207 | MS.gene04307:CDS |
| TTTGTTGGTACCGTCTCTTG+AGG | 0.616510 | 3.4:+56039735 | None:intergenic |
| CCGGCACCATGTCCGATCGT+CGG | 0.619938 | 3.4:-56046257 | MS.gene04307:CDS |
| CCAGGGAGAACTTGTAACTC+CGG | 0.620061 | 3.4:+56046207 | None:intergenic |
| TGATTCACATTGCGGTGGTG+TGG | 0.624689 | 3.4:+56046336 | None:intergenic |
| GGCAGAGAAATTCATTACAT+GGG | 0.625343 | 3.4:+56044876 | None:intergenic |
| TTGAATACAAGGAATCATTG+AGG | 0.627788 | 3.4:-56039343 | MS.gene04307:CDS |
| GGACATACCTTCTGTCAACA+TGG | 0.630760 | 3.4:-56044297 | MS.gene04307:CDS |
| AACTGGAATGAATCACGCCA+GGG | 0.631908 | 3.4:+56046190 | None:intergenic |
| GAATCCTGGAGGCAAGCATG+AGG | 0.641547 | 3.4:-56039969 | MS.gene04307:CDS |
| AATAGTTGTGTATACAGACT+CGG | 0.645056 | 3.4:-56043828 | MS.gene04307:CDS |
| ATATCTTAGTTATATGCATG+GGG | 0.647653 | 3.4:-56039813 | MS.gene04307:CDS |
| ACTGGTTAGACGAATCCCAT+CGG | 0.648981 | 3.4:+56046086 | None:intergenic |
| ACAAACGCCGAACTGAACAA+CGG | 0.666707 | 3.4:+56046114 | None:intergenic |
| AACCTTGAGATACCGAACAT+CGG | 0.667246 | 3.4:+56046159 | None:intergenic |
| ATGAAAGGAGATCAATAACA+TGG | 0.667767 | 3.4:+56043404 | None:intergenic |
| CGACGATTCGAAGTTTGCAA+CGG | 0.670188 | 3.4:+56046291 | None:intergenic |
| CAAGCTATAACCTCAAGAGA+CGG | 0.676408 | 3.4:-56039745 | MS.gene04307:CDS |
| GGAAACGCTGATTCACATTG+CGG | 0.692132 | 3.4:+56046328 | None:intergenic |
| AACGCTGATTCACATTGCGG+TGG | 0.693300 | 3.4:+56046331 | None:intergenic |
| TGGAAAGCACTTGTTAACAG+AGG | 0.695071 | 3.4:+56043197 | None:intergenic |
| TTAGACGAATCCCATCGGAA+AGG | 0.705477 | 3.4:+56046091 | None:intergenic |
| ACAAAACATAGAAAATGCAG+AGG | 0.706266 | 3.4:+56043095 | None:intergenic |
| AACAACGGCGAACCTACTGG+CGG | 0.710795 | 3.4:+56046129 | None:intergenic |
| AATCAACTTAAGCTGACGTG+AGG | 0.725978 | 3.4:+56044103 | None:intergenic |
| TCGTCCAGCTGGAATCCTGG+AGG | 0.728618 | 3.4:-56039980 | MS.gene04307:CDS |
| ACACTGCTGTTGCATCCAAG+TGG | 0.730685 | 3.4:+56039600 | None:intergenic |
| GTGTTACGGATATGCCACGA+AGG | 0.738897 | 3.4:+56046063 | None:intergenic |
| AGTTGTGTATACAGACTCGG+AGG | 0.742262 | 3.4:-56043825 | MS.gene04307:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 56039280 | 56046440 | 56039280 | ID=MS.gene04307 |
| chr3.4 | mRNA | 56039280 | 56046440 | 56039280 | ID=MS.gene04307.t1;Parent=MS.gene04307 |
| chr3.4 | exon | 56046070 | 56046440 | 56046070 | ID=MS.gene04307.t1.exon1;Parent=MS.gene04307.t1 |
| chr3.4 | CDS | 56046070 | 56046440 | 56046070 | ID=cds.MS.gene04307.t1;Parent=MS.gene04307.t1 |
| chr3.4 | exon | 56044813 | 56044943 | 56044813 | ID=MS.gene04307.t1.exon2;Parent=MS.gene04307.t1 |
| chr3.4 | CDS | 56044813 | 56044943 | 56044813 | ID=cds.MS.gene04307.t1;Parent=MS.gene04307.t1 |
| chr3.4 | exon | 56044097 | 56044422 | 56044097 | ID=MS.gene04307.t1.exon3;Parent=MS.gene04307.t1 |
| chr3.4 | CDS | 56044097 | 56044422 | 56044097 | ID=cds.MS.gene04307.t1;Parent=MS.gene04307.t1 |
| chr3.4 | exon | 56043805 | 56043918 | 56043805 | ID=MS.gene04307.t1.exon4;Parent=MS.gene04307.t1 |
| chr3.4 | CDS | 56043805 | 56043918 | 56043805 | ID=cds.MS.gene04307.t1;Parent=MS.gene04307.t1 |
| chr3.4 | exon | 56043365 | 56043491 | 56043365 | ID=MS.gene04307.t1.exon5;Parent=MS.gene04307.t1 |
| chr3.4 | CDS | 56043365 | 56043491 | 56043365 | ID=cds.MS.gene04307.t1;Parent=MS.gene04307.t1 |
| chr3.4 | exon | 56043012 | 56043282 | 56043012 | ID=MS.gene04307.t1.exon6;Parent=MS.gene04307.t1 |
| chr3.4 | CDS | 56043012 | 56043282 | 56043012 | ID=cds.MS.gene04307.t1;Parent=MS.gene04307.t1 |
| chr3.4 | exon | 56039280 | 56040003 | 56039280 | ID=MS.gene04307.t1.exon7;Parent=MS.gene04307.t1 |
| chr3.4 | CDS | 56039280 | 56040003 | 56039280 | ID=cds.MS.gene04307.t1;Parent=MS.gene04307.t1 |
| Gene Sequence |
| Protein sequence |
