Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058147.t1 | XP_003610912.2 | 94.3 | 788 | 37 | 1 | 1 | 788 | 1 | 780 | 4.50E-217 | 764.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058147.t1 | Q80WA4 | 34.1 | 173 | 108 | 3 | 199 | 368 | 55 | 224 | 1.0e-15 | 87.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058147.t1 | G7K7W5 | 94.3 | 788 | 37 | 1 | 1 | 788 | 1 | 780 | 3.2e-217 | 764.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051631 | MS.gene058147 | 0.801814 | 7.54E-49 | -1.69E-46 |
| MS.gene058147 | MS.gene88509 | 0.800998 | 1.11E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene04185 | MS.gene058147 | PPI |
| MS.gene024674 | MS.gene058147 | PPI |
| MS.gene072735 | MS.gene058147 | PPI |
| MS.gene05659 | MS.gene058147 | PPI |
| MS.gene058147 | MS.gene53118 | PPI |
| MS.gene058147 | MS.gene22316 | PPI |
| MS.gene85900 | MS.gene058147 | PPI |
| MS.gene78555 | MS.gene058147 | PPI |
| MS.gene55323 | MS.gene058147 | PPI |
| MS.gene21656 | MS.gene058147 | PPI |
| MS.gene04307 | MS.gene058147 | PPI |
| MS.gene94960 | MS.gene058147 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058147.t1 | MTR_5g008420 | 96.320 | 788 | 21 | 1 | 1 | 788 | 1 | 780 | 0.0 | 1486 |
| MS.gene058147.t1 | MTR_7g010340 | 57.670 | 678 | 133 | 17 | 1 | 669 | 1 | 533 | 0.0 | 628 |
| MS.gene058147.t1 | MTR_7g010340 | 56.047 | 678 | 128 | 17 | 1 | 669 | 1 | 517 | 0.0 | 600 |
| MS.gene058147.t1 | MTR_3g104280 | 41.843 | 521 | 231 | 25 | 193 | 674 | 86 | 573 | 7.57e-68 | 236 |
| MS.gene058147.t1 | MTR_3g104270 | 41.211 | 512 | 228 | 22 | 193 | 665 | 86 | 563 | 2.70e-66 | 231 |
| MS.gene058147.t1 | MTR_1g016760 | 47.273 | 275 | 117 | 9 | 193 | 459 | 112 | 366 | 3.31e-62 | 221 |
| MS.gene058147.t1 | MTR_7g010360 | 45.511 | 323 | 66 | 12 | 1 | 300 | 1 | 236 | 1.62e-45 | 175 |
| MS.gene058147.t1 | MTR_6g012630 | 27.174 | 184 | 115 | 7 | 187 | 360 | 42 | 216 | 4.71e-11 | 66.6 |
| MS.gene058147.t1 | MTR_6g012630 | 27.174 | 184 | 115 | 7 | 187 | 360 | 42 | 216 | 5.65e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058147.t1 | AT1G33680 | 42.212 | 841 | 324 | 33 | 1 | 758 | 1 | 762 | 1.18e-145 | 446 |
| MS.gene058147.t1 | AT4G10070 | 43.658 | 749 | 319 | 35 | 1 | 711 | 1 | 684 | 2.50e-134 | 414 |
| MS.gene058147.t1 | AT2G25970 | 47.479 | 238 | 123 | 2 | 193 | 429 | 134 | 370 | 8.25e-54 | 197 |
| MS.gene058147.t1 | AT5G50940 | 43.836 | 73 | 40 | 1 | 193 | 265 | 189 | 260 | 5.49e-12 | 67.4 |
Find 292 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCACCTGGTTGCCCATAAC+TGG | 0.089662 | 5.4:+3342450 | None:intergenic |
| TATGACTCAACCCAAGTTTA+TGG | 0.200615 | 5.4:-3342100 | MS.gene058147:CDS |
| TTGTTAGCGGCTGGTTGTTC+AGG | 0.233674 | 5.4:+3342355 | None:intergenic |
| GCCAGGCGAGATTCCGTATC+AGG | 0.249166 | 5.4:-3342641 | MS.gene058147:CDS |
| GTTCAGGTTGGCGTACTTAT+TGG | 0.253689 | 5.4:-3347052 | MS.gene058147:intron |
| CAGTTTATCACCTTCTCATT+TGG | 0.256871 | 5.4:+3346677 | None:intergenic |
| TGCTCAGTTGACTCCATTGA+TGG | 0.270905 | 5.4:+3349811 | None:intergenic |
| AAGAGCCTGCAGACCAAAAC+TGG | 0.283772 | 5.4:-3346542 | MS.gene058147:CDS |
| GGCTGTTCAGTTGCTTTAGA+TGG | 0.289174 | 5.4:+3349832 | None:intergenic |
| CAGCCTCAGGGTCAGGATTA+TGG | 0.301261 | 5.4:-3342919 | MS.gene058147:CDS |
| AAACTTGGGTTGAGTCATAT+TGG | 0.301611 | 5.4:+3342104 | None:intergenic |
| CTGGATGCGTGCCCCAGTTT+TGG | 0.305898 | 5.4:+3346529 | None:intergenic |
| TCATATGCACCTCCTCCTTA+TGG | 0.307453 | 5.4:-3343189 | MS.gene058147:CDS |
| ACTGAGCAAGACGCTTCTTC+TGG | 0.308215 | 5.4:-3349796 | MS.gene058147:CDS |
| ATCTGGTGGTCCACCCCAAT+GGG | 0.316449 | 5.4:-3343289 | MS.gene058147:CDS |
| ACAAGGCCTGTTGAACTAAT+AGG | 0.318881 | 5.4:-3346938 | MS.gene058147:CDS |
| GAAAGGACTGTCCAAGTTAC+TGG | 0.320619 | 5.4:-3345581 | MS.gene058147:CDS |
| CACTGTTGTAAGCAGGATCC+TGG | 0.326871 | 5.4:+3342308 | None:intergenic |
| CCTGGTGGACAGCCCAGTTA+TGG | 0.329458 | 5.4:-3342463 | MS.gene058147:CDS |
| TCCTTTCGAAAATGCTCCTT+TGG | 0.330000 | 5.4:-3349998 | MS.gene058147:CDS |
| CCAAGGAGCAGTTACGGTTC+TGG | 0.331193 | 5.4:-3343144 | MS.gene058147:CDS |
| GGCGTACTTATTGGCAAATC+TGG | 0.332530 | 5.4:-3347043 | MS.gene058147:CDS |
| GCTCAGATGAATTACAATTA+TGG | 0.338699 | 5.4:-3342943 | MS.gene058147:CDS |
| AGAAGGACCGGTACCATGTT+GGG | 0.340479 | 5.4:+3342993 | None:intergenic |
| GCAAATCTGGTGACACTATA+CGG | 0.340935 | 5.4:-3347030 | MS.gene058147:CDS |
| CGCTTACAATCAGAAGAAAC+AGG | 0.347578 | 5.4:+3351097 | None:intergenic |
| TTCTTTAGAATCATCGCCTT+CGG | 0.348747 | 5.4:+3345601 | None:intergenic |
| AAAGTTCCTATTAGTTCAAC+AGG | 0.349651 | 5.4:+3346932 | None:intergenic |
| ACGGTACCTGCAGTATAATT+CGG | 0.353164 | 5.4:-3347011 | MS.gene058147:CDS |
| TCCTTCTCCTTTACCCTCCA+TGG | 0.353311 | 5.4:-3342977 | MS.gene058147:CDS |
| GGGTAAGCTGGCTGAGGATT+TGG | 0.356446 | 5.4:+3342784 | None:intergenic |
| GGTCAGCGAGGTTCACATTA+TGG | 0.357233 | 5.4:-3343267 | MS.gene058147:CDS |
| ATGTCGTACCTAGGTCATTC+TGG | 0.361703 | 5.4:+3350957 | None:intergenic |
| CCATATCCATATGCATCATC+TGG | 0.361945 | 5.4:-3342580 | MS.gene058147:CDS |
| AGGTCATCATCAGGTGGTTT+TGG | 0.368182 | 5.4:-3343339 | MS.gene058147:CDS |
| TTACAGAATGACACTTCTTC+AGG | 0.372488 | 5.4:-3349736 | MS.gene058147:CDS |
| CAGGGGCCACCCTCACATAA+TGG | 0.372854 | 5.4:-3343093 | MS.gene058147:CDS |
| ATGCAGGTCGGGTTAATCAT+TGG | 0.377319 | 5.4:-3346584 | MS.gene058147:intron |
| AACTTGGGTTGAGTCATATT+GGG | 0.378107 | 5.4:+3342105 | None:intergenic |
| GCAGGAGCACCGGCTGCTGC+TGG | 0.382386 | 5.4:+3342169 | None:intergenic |
| GGGGACTGAACTGGGATGCT+GGG | 0.385001 | 5.4:+3343014 | None:intergenic |
| GCTGCATAGCCTGCTGGTTG+AGG | 0.385429 | 5.4:+3342196 | None:intergenic |
| CCAGATGATGCATATGGATA+TGG | 0.385841 | 5.4:+3342580 | None:intergenic |
| CTTTGCTGGAATAGCCTATC+AGG | 0.389163 | 5.4:-3343359 | MS.gene058147:intron |
| GCGCATAACTAGCTGTGGCC+TGG | 0.391139 | 5.4:+3342428 | None:intergenic |
| GTTGTTCAGGGTAAGTCTGC+TGG | 0.392931 | 5.4:+3342368 | None:intergenic |
| CAAGCAGCATATCCTACATA+TGG | 0.393896 | 5.4:-3342550 | MS.gene058147:CDS |
| AATGGTGGATATGATTATTA+TGG | 0.394577 | 5.4:-3343075 | MS.gene058147:CDS |
| TGCAGGTCGGGTTAATCATT+GGG | 0.396077 | 5.4:-3346583 | MS.gene058147:intron |
| AATCGAGGTTCCTAGCAATA+AGG | 0.397890 | 5.4:-3349671 | MS.gene058147:intron |
| CGCATAACTAGCTGTGGCCT+GGG | 0.398000 | 5.4:+3342429 | None:intergenic |
| GTGTTGGCAGCTAATACTAA+TGG | 0.398131 | 5.4:-3350072 | MS.gene058147:intron |
| TCCTTTGGAAAATGCTCCTT+CGG | 0.400218 | 5.4:-3349983 | MS.gene058147:CDS |
| CCATAACTGGGCTGTCCACC+AGG | 0.401150 | 5.4:+3342463 | None:intergenic |
| TCCTTACCAGAGTCCTCAAC+AGG | 0.405365 | 5.4:+3349868 | None:intergenic |
| CTAGCTGTGGCCTGGGCACC+TGG | 0.405662 | 5.4:+3342436 | None:intergenic |
| CTGCTGGTTGAGGGTTAGAT+TGG | 0.406286 | 5.4:+3342206 | None:intergenic |
| CTGGTGGACAGCCCAGTTAT+GGG | 0.408280 | 5.4:-3342462 | MS.gene058147:CDS |
| TACCGTCCGCCACGAGGATC+TGG | 0.408928 | 5.4:-3343306 | MS.gene058147:CDS |
| TCTTTAGAATCATCGCCTTC+GGG | 0.410748 | 5.4:+3345602 | None:intergenic |
| ATCGAGGAGCACCATAAACT+TGG | 0.411128 | 5.4:+3342089 | None:intergenic |
| AACTGGGCTGTCCACCAGGC+TGG | 0.412125 | 5.4:+3342467 | None:intergenic |
| TCGCTTGAGCACTGGCTTGA+GGG | 0.412219 | 5.4:+3342764 | None:intergenic |
| GGGTTAATCATTGGGAAAGG+TGG | 0.413875 | 5.4:-3346575 | MS.gene058147:CDS |
| TGAACCTCGCTGACCCCATT+GGG | 0.416626 | 5.4:+3343275 | None:intergenic |
| TCGCCTGGCTGAGTGGCTCT+AGG | 0.418678 | 5.4:+3342655 | None:intergenic |
| GCCTCTTATCACCAGTAACT+TGG | 0.424651 | 5.4:+3345570 | None:intergenic |
| CAACCCACACCAACTCAAAC+TGG | 0.425178 | 5.4:-3342235 | MS.gene058147:CDS |
| CAAGTTGGTCCTACTGGTTA+TGG | 0.425563 | 5.4:-3342406 | MS.gene058147:CDS |
| TAATTGTAATTCATCTGAGC+TGG | 0.428502 | 5.4:+3342946 | None:intergenic |
| TATGCACCTCCCCAGCAGTA+TGG | 0.428958 | 5.4:-3342739 | MS.gene058147:CDS |
| CGGTACCTGCAGTATAATTC+GGG | 0.432942 | 5.4:-3347010 | MS.gene058147:CDS |
| TGTTGAACTAATAGGAACTT+TGG | 0.433245 | 5.4:-3346930 | MS.gene058147:CDS |
| TTACAATTATGGACAGCCTC+AGG | 0.433579 | 5.4:-3342932 | MS.gene058147:CDS |
| GGATGTCCTGCCTGCTGATA+AGG | 0.436143 | 5.4:+3342886 | None:intergenic |
| GGACAACATCCACAGTCTTA+TGG | 0.440152 | 5.4:-3342682 | MS.gene058147:CDS |
| CATCAGCATCCCTAGTGATC+TGG | 0.442017 | 5.4:+3346978 | None:intergenic |
| TAAGGTGGGGTCAATAGAAC+TGG | 0.443962 | 5.4:+3349756 | None:intergenic |
| TACACCGTCAGTGTTGGATT+CGG | 0.445096 | 5.4:+3351045 | None:intergenic |
| ATCTCTTTGACATCCTCAAC+GGG | 0.445474 | 5.4:+3349892 | None:intergenic |
| GATTGATTATGAGAAGGATA+TGG | 0.449530 | 5.4:+3343210 | None:intergenic |
| TATGCGCAAGTTGGTCCTAC+TGG | 0.450191 | 5.4:-3342412 | MS.gene058147:CDS |
| GGTACAGCTGCATAGCCTGC+TGG | 0.451375 | 5.4:+3342190 | None:intergenic |
| GAAATTCCACAGGCTGATGC+TGG | 0.452709 | 5.4:-3346779 | MS.gene058147:intron |
| CAAAACTGGGGCACGCATCC+AGG | 0.456019 | 5.4:-3346528 | MS.gene058147:intron |
| CACCCTAGAGCCACTCAGCC+AGG | 0.456187 | 5.4:-3342658 | MS.gene058147:CDS |
| TGCATAATTCGCTTGAGCAC+TGG | 0.456659 | 5.4:+3342756 | None:intergenic |
| TATGGCAAGCCACCGTTGTA+TGG | 0.457045 | 5.4:-3342721 | MS.gene058147:CDS |
| GTGGCGGACGGTAGGCCTGC+TGG | 0.458635 | 5.4:+3343316 | None:intergenic |
| ACCGTCAGTGTTGGATTCGG+TGG | 0.459315 | 5.4:+3351048 | None:intergenic |
| ACGGTGGCTTGCCATACTGC+TGG | 0.460842 | 5.4:+3342728 | None:intergenic |
| CCCGCAGTCTGCACCTGGAC+AGG | 0.461205 | 5.4:-3342497 | MS.gene058147:CDS |
| CTCCCCTGCTCAGGCTATCG+TGG | 0.463470 | 5.4:-3346726 | MS.gene058147:CDS |
| GCCGCTAACAACGCATGTTA+TGG | 0.463733 | 5.4:-3342343 | MS.gene058147:CDS |
| CTGCATAGCCTGCTGGTTGA+GGG | 0.464272 | 5.4:+3342197 | None:intergenic |
| GAGCAGTTACGGTTCTGGTT+GGG | 0.467510 | 5.4:-3343139 | MS.gene058147:CDS |
| TCGAGGCCTCTCCCCTGCTC+AGG | 0.468246 | 5.4:-3346735 | MS.gene058147:CDS |
| GGACATCCTCAACAAGGTTA+TGG | 0.468279 | 5.4:-3342871 | MS.gene058147:CDS |
| TGGAAATTATCCTCAACATA+TGG | 0.468726 | 5.4:-3343169 | MS.gene058147:CDS |
| GCCGGTGCTCCTGCAGCATA+CGG | 0.469173 | 5.4:-3342160 | MS.gene058147:CDS |
| TGTTAGCGGCTGGTTGTTCA+GGG | 0.470020 | 5.4:+3342356 | None:intergenic |
| ATCCTCAACGGGAATCTCAT+TGG | 0.470692 | 5.4:+3349903 | None:intergenic |
| GGCTGGGCATAAACCTGTCC+AGG | 0.472801 | 5.4:+3342484 | None:intergenic |
| CCACCTGATGATGACCTGAT+AGG | 0.474279 | 5.4:+3343345 | None:intergenic |
| CGGTGGCTTGCCATACTGCT+GGG | 0.476941 | 5.4:+3342729 | None:intergenic |
| CCTCCATATGGATATTGTGC+AGG | 0.477000 | 5.4:+3342811 | None:intergenic |
| CCCTGATACGGAATCTCGCC+TGG | 0.477066 | 5.4:+3342640 | None:intergenic |
| AAGCAGGATCCTGGGGTGCT+TGG | 0.479506 | 5.4:+3342317 | None:intergenic |
| TTATGGACAGCCTCAGGGTC+AGG | 0.481108 | 5.4:-3342926 | MS.gene058147:CDS |
| GGTAAGTCTGCTGGGAAGAC+GGG | 0.482908 | 5.4:+3342377 | None:intergenic |
| GGATTTGGATGTCCTCCATA+TGG | 0.484661 | 5.4:+3342799 | None:intergenic |
| ACATAACCTGGCTGCACAGT+TGG | 0.486266 | 5.4:+3342259 | None:intergenic |
| GGGTCAATAGAACTGGAATC+AGG | 0.489780 | 5.4:+3349763 | None:intergenic |
| CGCCTGGCTGAGTGGCTCTA+GGG | 0.490124 | 5.4:+3342656 | None:intergenic |
| ACTGTTGTAAGCAGGATCCT+GGG | 0.490279 | 5.4:+3342309 | None:intergenic |
| CCAGAACCGTAACTGCTCCT+TGG | 0.490935 | 5.4:+3343144 | None:intergenic |
| GATCTGGTGGTCCACCCCAA+TGG | 0.493018 | 5.4:-3343290 | MS.gene058147:CDS |
| AGCAACTGAACAGCCATCAA+TGG | 0.493080 | 5.4:-3349824 | MS.gene058147:CDS |
| GCACCTGGTTGCCCATAACT+GGG | 0.497721 | 5.4:+3342451 | None:intergenic |
| GTGAACCTCGCTGACCCCAT+TGG | 0.497859 | 5.4:+3343274 | None:intergenic |
| CCTTCTCCTTTACCCTCCAT+GGG | 0.498897 | 5.4:-3342976 | MS.gene058147:CDS |
| CTGAGCTGGGGATGGTCCCA+TGG | 0.499599 | 5.4:+3342960 | None:intergenic |
| GAGATTCCTGTTGAGGACTC+TGG | 0.499644 | 5.4:-3349874 | MS.gene058147:CDS |
| CAGCCCAGTTATGGGCAACC+AGG | 0.499664 | 5.4:-3342454 | MS.gene058147:CDS |
| TTATGGGCAACCAGGTGCCC+AGG | 0.502933 | 5.4:-3342446 | MS.gene058147:CDS |
| TAACATGCGTTGTTAGCGGC+TGG | 0.503522 | 5.4:+3342346 | None:intergenic |
| GCACTGGCTTGAGGGTAAGC+TGG | 0.504108 | 5.4:+3342772 | None:intergenic |
| ATGGATATTGTGCAGGGCCA+CGG | 0.504139 | 5.4:+3342818 | None:intergenic |
| TCCAAGTTACTGGTGATAAG+AGG | 0.505553 | 5.4:-3345571 | MS.gene058147:CDS |
| TTCGCTTGAGCACTGGCTTG+AGG | 0.507075 | 5.4:+3342763 | None:intergenic |
| TCGTGTGGTTGTGTCAGACT+CGG | 0.507762 | 5.4:+3349696 | None:intergenic |
| GATGGTCCCATGGAGGGTAA+AGG | 0.507966 | 5.4:+3342970 | None:intergenic |
| GAAGAAACAGGGCTTGTAGC+TGG | 0.509067 | 5.4:+3351109 | None:intergenic |
| GGACAGGTTTATGCCCAGCC+TGG | 0.511456 | 5.4:-3342481 | MS.gene058147:CDS |
| CGATAGCCTGAGCAGGGGAG+AGG | 0.512286 | 5.4:+3346729 | None:intergenic |
| TGAAGAAGTGTCATTCTGTA+AGG | 0.512662 | 5.4:+3349738 | None:intergenic |
| TTGTTCAGGGTAAGTCTGCT+GGG | 0.512955 | 5.4:+3342369 | None:intergenic |
| TGTTGAGGATAATTTCCATA+AGG | 0.513807 | 5.4:+3343174 | None:intergenic |
| TCTACTCCAGCACAATCATA+TGG | 0.514595 | 5.4:-3342616 | MS.gene058147:CDS |
| GGCCCTGCACAATATCCATA+TGG | 0.514725 | 5.4:-3342814 | MS.gene058147:CDS |
| ATGGCTCCAAGGAGCAGTTA+CGG | 0.518058 | 5.4:-3343150 | MS.gene058147:CDS |
| ACTGTCTTGCCGTATGCTGC+AGG | 0.519390 | 5.4:+3342151 | None:intergenic |
| GTGCAAAGATCCAGATCACT+AGG | 0.520840 | 5.4:-3346988 | MS.gene058147:CDS |
| GCCGTATGCTGCAGGAGCAC+CGG | 0.521123 | 5.4:+3342159 | None:intergenic |
| GAGAAGGACCGGTACCATGT+TGG | 0.521552 | 5.4:+3342992 | None:intergenic |
| TAGCCTATCAGGTCATCATC+AGG | 0.521896 | 5.4:-3343348 | MS.gene058147:CDS |
| AGGCCTCGAGCTACAAGTGC+AGG | 0.522201 | 5.4:+3346749 | None:intergenic |
| TGGGCATAAGCAGCGGGCTG+AGG | 0.523158 | 5.4:+3342124 | None:intergenic |
| GCAGCTGTACCAGCAGCAGC+CGG | 0.525098 | 5.4:-3342178 | MS.gene058147:CDS |
| TACTAATGGACATCAAGAAA+AGG | 0.526778 | 5.4:-3350058 | MS.gene058147:CDS |
| CCTGTCCAGGTGCAGACTGC+GGG | 0.527053 | 5.4:+3342497 | None:intergenic |
| AGTCACCCGCAGTCTGCACC+TGG | 0.528460 | 5.4:-3342502 | MS.gene058147:CDS |
| GGGTAAGTCTGCTGGGAAGA+CGG | 0.528672 | 5.4:+3342376 | None:intergenic |
| CGGAATCTCGCCTGGCTGAG+TGG | 0.531568 | 5.4:+3342648 | None:intergenic |
| GGTTAATCATTGGGAAAGGT+GGG | 0.531773 | 5.4:-3346574 | MS.gene058147:CDS |
| AATTGTAATTCATCTGAGCT+GGG | 0.532849 | 5.4:+3342947 | None:intergenic |
| ACGGTTCTGGTTGGGAGCAA+AGG | 0.533493 | 5.4:-3343131 | MS.gene058147:CDS |
| GGCCCCTGAAAACTCTGGTG+AGG | 0.534001 | 5.4:+3343108 | None:intergenic |
| AGTTTCTACACCGTCAGTGT+TGG | 0.535493 | 5.4:+3351039 | None:intergenic |
| AATACACATACCTTATTGCT+AGG | 0.536065 | 5.4:+3349661 | None:intergenic |
| GATAAAAGAAGTCTTGAGCC+AGG | 0.537717 | 5.4:-3345525 | MS.gene058147:intron |
| ACTGGGCTGTCCACCAGGCT+GGG | 0.539109 | 5.4:+3342468 | None:intergenic |
| CCTAGCTATGGCTGAAGAAG+AGG | 0.540273 | 5.4:-3351146 | None:intergenic |
| GTAACCAGTTTGAGTTGGTG+TGG | 0.543877 | 5.4:+3342231 | None:intergenic |
| GATCCCACGATAGCCTGAGC+AGG | 0.544382 | 5.4:+3346722 | None:intergenic |
| TCCTGTTGAGGACTCTGGTA+AGG | 0.544945 | 5.4:-3349869 | MS.gene058147:CDS |
| TCCCCTGCTCAGGCTATCGT+GGG | 0.545298 | 5.4:-3346725 | MS.gene058147:CDS |
| GAAGTGTCATTCTGTAAGGT+GGG | 0.546448 | 5.4:+3349742 | None:intergenic |
| CTGGGATGCTGGGCAGATGA+AGG | 0.550410 | 5.4:+3343024 | None:intergenic |
| TCATCAGACACCTTATCAGC+AGG | 0.553463 | 5.4:-3342896 | MS.gene058147:CDS |
| CCTCGCTGACCCCATTGGGG+TGG | 0.554723 | 5.4:+3343279 | None:intergenic |
| GATGGCCCAGATGATGCATA+TGG | 0.555394 | 5.4:+3342574 | None:intergenic |
| AGCTGGGGATGGTCCCATGG+AGG | 0.556204 | 5.4:+3342963 | None:intergenic |
| GCAGCTCCAACTGTGCAGCC+AGG | 0.558834 | 5.4:-3342265 | MS.gene058147:CDS |
| GGAGCAGTTACGGTTCTGGT+TGG | 0.559653 | 5.4:-3343140 | MS.gene058147:CDS |
| CCGTCAGTGTTGGATTCGGT+GGG | 0.560541 | 5.4:+3351049 | None:intergenic |
| GGTGGATATGATTATTATGG+TGG | 0.562699 | 5.4:-3343072 | MS.gene058147:CDS |
| CAATCTAACCCTCAACCAGC+AGG | 0.562879 | 5.4:-3342205 | MS.gene058147:CDS |
| TCATATTGGGCATAAGCAGC+GGG | 0.563343 | 5.4:+3342118 | None:intergenic |
| TAACCAGTTTGAGTTGGTGT+GGG | 0.565713 | 5.4:+3342232 | None:intergenic |
| AAGCAGCATATCCTACATAT+GGG | 0.567067 | 5.4:-3342549 | MS.gene058147:CDS |
| TTATGGCTACCAAGCACCCC+AGG | 0.569924 | 5.4:-3342326 | MS.gene058147:CDS |
| GAAGGACCGGTACCATGTTG+GGG | 0.570002 | 5.4:+3342994 | None:intergenic |
| GCAGCGCCACTGTTGTAAGC+AGG | 0.571040 | 5.4:+3342301 | None:intergenic |
| CTCCTTGGAGCCATATGTTG+AGG | 0.573692 | 5.4:+3343159 | None:intergenic |
| AAGGCGATGATTCTAAAGAA+AGG | 0.574886 | 5.4:-3345598 | MS.gene058147:CDS |
| AGAGCCTGCAGACCAAAACT+GGG | 0.576264 | 5.4:-3346541 | MS.gene058147:CDS |
| AGATCCTCGTGGCGGACGGT+AGG | 0.576333 | 5.4:+3343308 | None:intergenic |
| CATATCCATATGCATCATCT+GGG | 0.577251 | 5.4:-3342579 | MS.gene058147:CDS |
| AGAAGTGTCATTCTGTAAGG+TGG | 0.579993 | 5.4:+3349741 | None:intergenic |
| TGACGCCGCTGCAGTTGCTG+AGG | 0.581663 | 5.4:-3351017 | MS.gene058147:CDS |
| TAATTCATCTGAGCTGGGGA+TGG | 0.582659 | 5.4:+3342952 | None:intergenic |
| CTGTTGTAAGCAGGATCCTG+GGG | 0.583236 | 5.4:+3342310 | None:intergenic |
| CACCAGATCCTCGTGGCGGA+CGG | 0.583322 | 5.4:+3343304 | None:intergenic |
| GGATGTTGTCCTTGTGAAGG+TGG | 0.583512 | 5.4:+3342694 | None:intergenic |
| CCCATGGAGGGTAAAGGAGA+AGG | 0.583548 | 5.4:+3342976 | None:intergenic |
| TTTGGAAAATGCTCCTTCGG+AGG | 0.583555 | 5.4:-3349980 | MS.gene058147:CDS |
| GGATAATTTCCATAAGGAGG+AGG | 0.583935 | 5.4:+3343180 | None:intergenic |
| TGGTCGTAACCAGTTTGAGT+TGG | 0.584239 | 5.4:+3342226 | None:intergenic |
| CATATGACTATCAGCACCGC+GGG | 0.584630 | 5.4:-3343233 | MS.gene058147:CDS |
| CTCCATATGGATATTGTGCA+GGG | 0.585287 | 5.4:+3342812 | None:intergenic |
| GGAGGGTAAAGGAGAAGGAC+CGG | 0.585487 | 5.4:+3342981 | None:intergenic |
| GTTGTGTCAGACTCGGATAT+AGG | 0.586274 | 5.4:+3349703 | None:intergenic |
| CGGTGCTGATAGTCATATGA+TGG | 0.586322 | 5.4:+3343237 | None:intergenic |
| TGACTGTAACCATCTGCAGC+TGG | 0.588200 | 5.4:+3342520 | None:intergenic |
| TGTCAAAGAGATTCCTGTTG+AGG | 0.588403 | 5.4:-3349881 | MS.gene058147:CDS |
| CCTTGTCCATAACCTTGTTG+AGG | 0.589130 | 5.4:+3342865 | None:intergenic |
| GCTTGAGGGTAAGCTGGCTG+AGG | 0.589420 | 5.4:+3342778 | None:intergenic |
| GGCTTGCCATACTGCTGGGG+AGG | 0.589568 | 5.4:+3342733 | None:intergenic |
| GCCATAACATGCGTTGTTAG+CGG | 0.589936 | 5.4:+3342342 | None:intergenic |
| GCTGGGGATGGTCCCATGGA+GGG | 0.591933 | 5.4:+3342964 | None:intergenic |
| GCTGGTGCAGACCCATATGT+AGG | 0.592494 | 5.4:+3342538 | None:intergenic |
| ATCCCACGATAGCCTGAGCA+GGG | 0.592936 | 5.4:+3346723 | None:intergenic |
| ATCATATCCACCATTATGTG+AGG | 0.594833 | 5.4:+3343083 | None:intergenic |
| AATCTCTTTGACATCCTCAA+CGG | 0.595049 | 5.4:+3349891 | None:intergenic |
| ACCTGTCCAGGTGCAGACTG+CGG | 0.595220 | 5.4:+3342496 | None:intergenic |
| TACAATTATGGACAGCCTCA+GGG | 0.595891 | 5.4:-3342931 | MS.gene058147:CDS |
| GTCATATTGGGCATAAGCAG+CGG | 0.596829 | 5.4:+3342117 | None:intergenic |
| GATACAAGTTCCAAATGAGA+AGG | 0.597304 | 5.4:-3346687 | MS.gene058147:intron |
| TCGCCTTCGGGAAGATGTTG+AGG | 0.598864 | 5.4:+3345614 | None:intergenic |
| GTAGGATATGCTGCTTGTGA+TGG | 0.599015 | 5.4:+3342556 | None:intergenic |
| ACAGGCCTTGTTGAAGAGTG+AGG | 0.603130 | 5.4:+3346950 | None:intergenic |
| ATCCTCAACATATGGCTCCA+AGG | 0.603445 | 5.4:-3343161 | MS.gene058147:CDS |
| GGGTCTGCACCAGCTGCAGA+TGG | 0.603725 | 5.4:-3342529 | MS.gene058147:CDS |
| TTGCACCCGAATTATACTGC+AGG | 0.604515 | 5.4:+3347005 | None:intergenic |
| CTGGCTGAGTGGCTCTAGGG+TGG | 0.605118 | 5.4:+3342659 | None:intergenic |
| CTCTGGTAAGGAAAATGACA+AGG | 0.605178 | 5.4:-3349857 | MS.gene058147:CDS |
| CCAGGCGAGATTCCGTATCA+GGG | 0.607791 | 5.4:-3342640 | MS.gene058147:CDS |
| TGAGGATAATTTCCATAAGG+AGG | 0.609076 | 5.4:+3343177 | None:intergenic |
| CCTCAACAAGGTTATGGACA+AGG | 0.609190 | 5.4:-3342865 | MS.gene058147:CDS |
| TGGTTATCGTCGTCAAGACG+AGG | 0.613071 | 5.4:+3350977 | None:intergenic |
| CAGGTTTATGCCCAGCCTGG+TGG | 0.613647 | 5.4:-3342478 | MS.gene058147:CDS |
| AACTTGCGCATAACTAGCTG+TGG | 0.614751 | 5.4:+3342423 | None:intergenic |
| GCATATGATTGATTATGAGA+AGG | 0.617478 | 5.4:+3343204 | None:intergenic |
| CGTCCGCCACGAGGATCTGG+TGG | 0.619194 | 5.4:-3343303 | MS.gene058147:CDS |
| CTGATCCTCACTCTTCAACA+AGG | 0.619869 | 5.4:-3346955 | MS.gene058147:CDS |
| GTCGGGTTAATCATTGGGAA+AGG | 0.620723 | 5.4:-3346578 | MS.gene058147:CDS |
| ACATCTTGAACTTCCTCCGA+AGG | 0.620832 | 5.4:+3349967 | None:intergenic |
| TCGAGGAGCACCATAAACTT+GGG | 0.623385 | 5.4:+3342090 | None:intergenic |
| CAGACACCTTATCAGCAGGC+AGG | 0.625185 | 5.4:-3342892 | MS.gene058147:CDS |
| ACAGCTAGTTATGCGCAAGT+TGG | 0.627057 | 5.4:-3342421 | MS.gene058147:CDS |
| GGAATCTCATTGGCATCAGT+AGG | 0.627548 | 5.4:+3349913 | None:intergenic |
| GTTAATCATTGGGAAAGGTG+GGG | 0.628317 | 5.4:-3346573 | MS.gene058147:CDS |
| GGTGTGGGTTGAACATAACC+TGG | 0.628424 | 5.4:+3342247 | None:intergenic |
| TGTGGATGTTGTCCTTGTGA+AGG | 0.628816 | 5.4:+3342691 | None:intergenic |
| GCTGGAGTAGAACCCTGATA+CGG | 0.630359 | 5.4:+3342628 | None:intergenic |
| TCAAGAAAAGGAAGTTGCTG+AGG | 0.630607 | 5.4:-3350046 | MS.gene058147:CDS |
| ACTGTGCCATATGATTGTGC+TGG | 0.633161 | 5.4:+3342610 | None:intergenic |
| AGGGTGGCCCCTGAAAACTC+TGG | 0.635256 | 5.4:+3343103 | None:intergenic |
| GGTGGCTTGCCATACTGCTG+GGG | 0.637360 | 5.4:+3342730 | None:intergenic |
| CAGGATCCTGCTTACAACAG+TGG | 0.637586 | 5.4:-3342307 | MS.gene058147:CDS |
| ATCGTCCTCAGCAACTGCAG+CGG | 0.638462 | 5.4:+3351012 | None:intergenic |
| TCATATCCACCATTATGTGA+GGG | 0.639095 | 5.4:+3343084 | None:intergenic |
| ATTCCACAGGCTGATGCTGG+AGG | 0.639608 | 5.4:-3346776 | MS.gene058147:intron |
| GATTATTATGGTGGACAAAG+CGG | 0.641921 | 5.4:-3343063 | MS.gene058147:CDS |
| AAGGACCGGTACCATGTTGG+GGG | 0.643168 | 5.4:+3342995 | None:intergenic |
| GATAGTCATATGATGGTGGT+TGG | 0.644576 | 5.4:+3343244 | None:intergenic |
| TCTCCTGCACTTGTAGCTCG+AGG | 0.645902 | 5.4:-3346752 | MS.gene058147:CDS |
| TATGGTGTGCCACCTTCACA+AGG | 0.647766 | 5.4:-3342703 | MS.gene058147:CDS |
| AACTTTGGAAAGCATAGACA+AGG | 0.648448 | 5.4:-3346915 | MS.gene058147:CDS |
| GGGCCACCCTCACATAATGG+TGG | 0.651219 | 5.4:-3343090 | MS.gene058147:CDS |
| CAGGCAGGACATCCTCAACA+AGG | 0.654329 | 5.4:-3342877 | MS.gene058147:CDS |
| ATTGTAATTCATCTGAGCTG+GGG | 0.655860 | 5.4:+3342948 | None:intergenic |
| TATCCACCATTATGTGAGGG+TGG | 0.659251 | 5.4:+3343087 | None:intergenic |
| ATACCTCAACATCTTCCCGA+AGG | 0.661549 | 5.4:-3345617 | MS.gene058147:CDS |
| CAGGCCTACCGTCCGCCACG+AGG | 0.664533 | 5.4:-3343312 | MS.gene058147:CDS |
| TGCTGATAGTCATATGATGG+TGG | 0.665307 | 5.4:+3343240 | None:intergenic |
| CTAGGGTGGCCATAAGACTG+TGG | 0.667005 | 5.4:+3342673 | None:intergenic |
| CCACCCCAATGGGGTCAGCG+AGG | 0.668636 | 5.4:-3343279 | MS.gene058147:CDS |
| GGGTATGATCCATAACCAGT+AGG | 0.670355 | 5.4:+3342397 | None:intergenic |
| TCCCACGATAGCCTGAGCAG+GGG | 0.672197 | 5.4:+3346724 | None:intergenic |
| TGCAAAGATCCAGATCACTA+GGG | 0.672341 | 5.4:-3346987 | MS.gene058147:CDS |
| CCTGCACAATATCCATATGG+AGG | 0.672775 | 5.4:-3342811 | MS.gene058147:CDS |
| TGCCAATGAGATTCCCGTTG+AGG | 0.673173 | 5.4:-3349905 | MS.gene058147:CDS |
| GCTTACAATCAGAAGAAACA+GGG | 0.675017 | 5.4:+3351098 | None:intergenic |
| CCCACCGAATCCAACACTGA+CGG | 0.677283 | 5.4:-3351049 | MS.gene058147:CDS |
| TGAGGCAGAAACAGAAACAG+AGG | 0.677467 | 5.4:-3350028 | MS.gene058147:CDS |
| CCTATCAGGTCATCATCAGG+TGG | 0.685141 | 5.4:-3343345 | MS.gene058147:CDS |
| AAGTGTCATTCTGTAAGGTG+GGG | 0.686055 | 5.4:+3349743 | None:intergenic |
| GACGATAACCAGAATGACCT+AGG | 0.692835 | 5.4:-3350965 | MS.gene058147:intron |
| GAACCTCGCTGACCCCATTG+GGG | 0.701978 | 5.4:+3343276 | None:intergenic |
| TATGAGAAGGATATGGCCCG+CGG | 0.706134 | 5.4:+3343217 | None:intergenic |
| GAATCGAAGTACGAAAACCG+TGG | 0.723766 | 5.4:-3342835 | MS.gene058147:CDS |
| GAAGGTGGCACACCATACAA+CGG | 0.724316 | 5.4:+3342709 | None:intergenic |
| TCTGGTGGTCCACCCCAATG+GGG | 0.728168 | 5.4:-3343288 | MS.gene058147:CDS |
| GGTGGACCACCAGATCCTCG+TGG | 0.736923 | 5.4:+3343297 | None:intergenic |
| AATGAATGCTGTGATTGCAG+AGG | 0.739384 | 5.4:-3346882 | MS.gene058147:intron |
| TGACCATAATCCTGACCCTG+AGG | 0.742155 | 5.4:+3342916 | None:intergenic |
| GGTGGCACACCATACAACGG+TGG | 0.744350 | 5.4:+3342712 | None:intergenic |
| TCATATGACTATCAGCACCG+CGG | 0.747919 | 5.4:-3343234 | MS.gene058147:CDS |
| GAGCCTGCAGACCAAAACTG+GGG | 0.750434 | 5.4:-3346540 | MS.gene058147:CDS |
| GGACCACCAGATCCTCGTGG+CGG | 0.754108 | 5.4:+3343300 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 3342087 | 3351161 | 3342087 | ID=MS.gene058147 |
| chr5.4 | mRNA | 3342087 | 3351161 | 3342087 | ID=MS.gene058147.t1;Parent=MS.gene058147 |
| chr5.4 | exon | 3350966 | 3351161 | 3350966 | ID=MS.gene058147.t1.exon1;Parent=MS.gene058147.t1 |
| chr5.4 | CDS | 3350966 | 3351161 | 3350966 | ID=cds.MS.gene058147.t1;Parent=MS.gene058147.t1 |
| chr5.4 | exon | 3349672 | 3350084 | 3349672 | ID=MS.gene058147.t1.exon2;Parent=MS.gene058147.t1 |
| chr5.4 | CDS | 3349672 | 3350084 | 3349672 | ID=cds.MS.gene058147.t1;Parent=MS.gene058147.t1 |
| chr5.4 | exon | 3346883 | 3347068 | 3346883 | ID=MS.gene058147.t1.exon3;Parent=MS.gene058147.t1 |
| chr5.4 | CDS | 3346883 | 3347068 | 3346883 | ID=cds.MS.gene058147.t1;Parent=MS.gene058147.t1 |
| chr5.4 | exon | 3346688 | 3346789 | 3346688 | ID=MS.gene058147.t1.exon4;Parent=MS.gene058147.t1 |
| chr5.4 | CDS | 3346688 | 3346789 | 3346688 | ID=cds.MS.gene058147.t1;Parent=MS.gene058147.t1 |
| chr5.4 | exon | 3346529 | 3346600 | 3346529 | ID=MS.gene058147.t1.exon5;Parent=MS.gene058147.t1 |
| chr5.4 | CDS | 3346529 | 3346600 | 3346529 | ID=cds.MS.gene058147.t1;Parent=MS.gene058147.t1 |
| chr5.4 | exon | 3345526 | 3345642 | 3345526 | ID=MS.gene058147.t1.exon6;Parent=MS.gene058147.t1 |
| chr5.4 | CDS | 3345526 | 3345642 | 3345526 | ID=cds.MS.gene058147.t1;Parent=MS.gene058147.t1 |
| chr5.4 | exon | 3342087 | 3343367 | 3342087 | ID=MS.gene058147.t1.exon7;Parent=MS.gene058147.t1 |
| chr5.4 | CDS | 3342087 | 3343367 | 3342087 | ID=cds.MS.gene058147.t1;Parent=MS.gene058147.t1 |
| Gene Sequence |
| Protein sequence |
