Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043157.t1 | XP_013455261.1 | 94.8 | 618 | 9 | 1 | 1 | 595 | 1 | 618 | 0.00E+00 | 1155.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043157.t1 | Q291H5 | 56.6 | 438 | 178 | 4 | 25 | 453 | 66 | 500 | 1.7e-140 | 501.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043157.t1 | A0A072UHZ4 | 94.8 | 618 | 9 | 1 | 1 | 595 | 1 | 618 | 0.0e+00 | 1155.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene85145 | MS.gene043157 | PPI |
| MS.gene043157 | MS.gene025347 | PPI |
| MS.gene043157 | MS.gene062116 | PPI |
| MS.gene069865 | MS.gene043157 | PPI |
| MS.gene36347 | MS.gene043157 | PPI |
| MS.gene043157 | MS.gene85342 | PPI |
| MS.gene043157 | MS.gene76530 | PPI |
| MS.gene36348 | MS.gene043157 | PPI |
| MS.gene85146 | MS.gene043157 | PPI |
| MS.gene043157 | MS.gene070139 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043157.t1 | MTR_4g033210 | 94.822 | 618 | 9 | 1 | 1 | 595 | 1 | 618 | 0.0 | 1199 |
| MS.gene043157.t1 | MTR_5g021230 | 24.303 | 502 | 292 | 15 | 27 | 452 | 97 | 586 | 4.18e-33 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043157.t1 | AT1G72090 | 65.605 | 628 | 151 | 6 | 1 | 592 | 1 | 599 | 0.0 | 810 |
| MS.gene043157.t1 | AT4G36390 | 23.952 | 501 | 292 | 16 | 29 | 452 | 129 | 617 | 1.61e-31 | 130 |
Find 126 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGTTGATAAGGCTCTTT+TGG | 0.133036 | 4.2:-71557676 | MS.gene043157:CDS |
| GCTGGTCAGCTTTCGGCTTT+TGG | 0.175215 | 4.2:-71564164 | MS.gene043157:CDS |
| CCAATTAATGTTGGTTGTTT+AGG | 0.201753 | 4.2:-71561578 | MS.gene043157:CDS |
| CCTAAACAACCAACATTAAT+TGG | 0.244538 | 4.2:+71561578 | None:intergenic |
| AAGGCAAAGTGGAAAGAATT+TGG | 0.247160 | 4.2:-71558840 | MS.gene043157:CDS |
| GTCTTAGGTTTAGTGTTCTT+TGG | 0.248640 | 4.2:-71557608 | MS.gene043157:CDS |
| TTGCTTTATTAGTTTGTCTT+AGG | 0.260565 | 4.2:-71557623 | MS.gene043157:CDS |
| CTTCTCTTCACCACATTACT+TGG | 0.264756 | 4.2:+71558916 | None:intergenic |
| TTCAAGTGCTCAAGGATAAA+AGG | 0.293438 | 4.2:+71560696 | None:intergenic |
| GATATAATATGTGGATTTCC+AGG | 0.335774 | 4.2:-71560425 | MS.gene043157:intron |
| TTCCCACAAGCACAAGATTC+TGG | 0.347283 | 4.2:+71557897 | None:intergenic |
| TTCTGAGATTTCACAAGTCT+TGG | 0.354694 | 4.2:+71557923 | None:intergenic |
| ACCTGCGGCAAGAATGAAAA+AGG | 0.355796 | 4.2:-71558941 | MS.gene043157:CDS |
| CTGATGGAGTTAAAGAAATA+TGG | 0.362385 | 4.2:-71561268 | MS.gene043157:CDS |
| ATATATGGCTGGTCAGCTTT+CGG | 0.372540 | 4.2:-71564171 | MS.gene043157:CDS |
| AATCTTCCAACTTTATTGAA+TGG | 0.375607 | 4.2:-71560780 | MS.gene043157:CDS |
| TTGCCTTCCATCCCAGAGTA+TGG | 0.378724 | 4.2:+71558856 | None:intergenic |
| CTGAACTGGCACATGCAAAA+AGG | 0.379014 | 4.2:+71560635 | None:intergenic |
| ACCTTAACTGAGCTTGTGCC+AGG | 0.381985 | 4.2:-71560464 | MS.gene043157:CDS |
| CAAAGACATTTGTCAATTCA+CGG | 0.382984 | 4.2:+71558890 | None:intergenic |
| CTGGAAGGGATCAGCATTGT+AGG | 0.383351 | 4.2:-71561859 | MS.gene043157:CDS |
| ATCCTGATGAAGCAGATCTA+TGG | 0.386251 | 4.2:-71564124 | MS.gene043157:CDS |
| GAATCATTCACGCCATACTC+TGG | 0.389654 | 4.2:-71558868 | MS.gene043157:CDS |
| AAAGGAAGAATCTGCACAAA+AGG | 0.397088 | 4.2:-71557761 | MS.gene043157:CDS |
| GTTGTAGAGGAGACTTTAAA+AGG | 0.399227 | 4.2:-71561808 | MS.gene043157:CDS |
| TACAGTGTCCCTTGAATTGT+CGG | 0.404396 | 4.2:+71557836 | None:intergenic |
| CCTTCCCACAGATGTTATCT+TGG | 0.407575 | 4.2:+71558055 | None:intergenic |
| GTAGAGTGATAGTGAATATA+TGG | 0.408962 | 4.2:-71564186 | MS.gene043157:intron |
| AGATTTCACAAGTCTTGGTT+TGG | 0.413329 | 4.2:+71557928 | None:intergenic |
| CAACAACACGATCAATTTGC+TGG | 0.415734 | 4.2:+71561833 | None:intergenic |
| AAGAAAGGAGCATGAGAAAG+TGG | 0.418448 | 4.2:-71557701 | MS.gene043157:CDS |
| AATAAACCACTAGTTGTTGC+TGG | 0.419430 | 4.2:-71561916 | MS.gene043157:CDS |
| AGCCGCTACCGGAGCTCCAC+CGG | 0.422571 | 4.2:-71564546 | MS.gene043157:CDS |
| CCCTGGCACAAGCTCAGTTA+AGG | 0.428506 | 4.2:+71560463 | None:intergenic |
| AGCCATAGATCTGCTTCATC+AGG | 0.432713 | 4.2:+71564122 | None:intergenic |
| ACTAAGCCATTCAATAAAGT+TGG | 0.436328 | 4.2:+71560774 | None:intergenic |
| AGCATAGTGCTTGCATCAGC+TGG | 0.438177 | 4.2:+71560741 | None:intergenic |
| TTGAGAAGACCGACAATTCA+AGG | 0.440687 | 4.2:-71557845 | MS.gene043157:CDS |
| AAAGTGGGACTTTGTTGATA+AGG | 0.441838 | 4.2:-71557685 | MS.gene043157:CDS |
| TTGCAAGACAAAACATGCTC+GGG | 0.450430 | 4.2:-71561543 | MS.gene043157:CDS |
| TTGGATGGATACTGAGGAAA+AGG | 0.450909 | 4.2:-71557779 | MS.gene043157:CDS |
| TTGCATGTGCCAGTTCAGTC+TGG | 0.452847 | 4.2:-71560630 | MS.gene043157:CDS |
| TCACATCTCGACTTCCTTGA+GGG | 0.467101 | 4.2:+71561887 | None:intergenic |
| TCCCGGTGGAGCTCCGGTAG+CGG | 0.470158 | 4.2:+71564544 | None:intergenic |
| AGTGAAGATACCGGAGCATA+TGG | 0.472936 | 4.2:-71561239 | MS.gene043157:intron |
| ATGGATACCATCATAACAAA+AGG | 0.483104 | 4.2:-71561949 | MS.gene043157:CDS |
| ACGGAATCCCGGTGGAGCTC+CGG | 0.484772 | 4.2:+71564538 | None:intergenic |
| TTCAACGGAGAACGGAATCC+CGG | 0.485616 | 4.2:+71564527 | None:intergenic |
| GAACTTCCAAGCATATCATC+TGG | 0.486695 | 4.2:+71558086 | None:intergenic |
| GCATCACTTCCAGACTGAAC+TGG | 0.492471 | 4.2:+71560621 | None:intergenic |
| TTGCTCGTCGGAGCCGCTAC+CGG | 0.494283 | 4.2:-71564557 | MS.gene043157:CDS |
| GCAAGCACTATGCTGCGTAT+TGG | 0.495273 | 4.2:-71560732 | MS.gene043157:CDS |
| GATCTGATCTAAAGCAAGAA+AGG | 0.497761 | 4.2:-71557716 | MS.gene043157:CDS |
| CTGAAGGTTGGTCATACAAA+GGG | 0.500724 | 4.2:-71558134 | MS.gene043157:intron |
| CTCAAGGATAAAAGGTGGAT+TGG | 0.509294 | 4.2:+71560704 | None:intergenic |
| TTCACATCTCGACTTCCTTG+AGG | 0.511443 | 4.2:+71561886 | None:intergenic |
| GAGCCAGAATCTTGTGCTTG+TGG | 0.516083 | 4.2:-71557900 | MS.gene043157:CDS |
| AGCCAGAATCTTGTGCTTGT+GGG | 0.517247 | 4.2:-71557899 | MS.gene043157:CDS |
| GAACAAGCAGGTTCCTACTC+AGG | 0.517806 | 4.2:-71557973 | MS.gene043157:CDS |
| GTATCCATGGCAGATTGACT+TGG | 0.520909 | 4.2:+71561964 | None:intergenic |
| TGCAACTGATGGAGTTCACT+TGG | 0.521540 | 4.2:-71558806 | MS.gene043157:intron |
| ATTGCCACTGATATAATATG+TGG | 0.524293 | 4.2:-71560434 | MS.gene043157:CDS |
| TATCCATGGCAGATTGACTT+GGG | 0.524481 | 4.2:+71561965 | None:intergenic |
| AATCATTCACGCCATACTCT+GGG | 0.529461 | 4.2:-71558867 | MS.gene043157:CDS |
| AAGTCGAGATGTGAAAGAGC+TGG | 0.532706 | 4.2:-71561878 | MS.gene043157:CDS |
| ATTACTGATATTGCAACTGA+TGG | 0.536300 | 4.2:-71558817 | MS.gene043157:CDS |
| CAATAAAGCTTTGAACAAGC+AGG | 0.540064 | 4.2:-71557985 | MS.gene043157:CDS |
| TCAGTGGCAATCTGCATCCC+TGG | 0.542219 | 4.2:+71560446 | None:intergenic |
| CTTTAAAAGGTCACGAAGTG+AGG | 0.543654 | 4.2:-71561795 | MS.gene043157:CDS |
| CTTCTTCGTTGCAACGCCGA+CGG | 0.544956 | 4.2:+71564496 | None:intergenic |
| ATTGCTCCAGATGATATGCT+TGG | 0.546633 | 4.2:-71558092 | MS.gene043157:CDS |
| CTATCTCTTTCAAGTGCTCA+AGG | 0.546786 | 4.2:+71560688 | None:intergenic |
| TATCTATTGTGTAACTCCCT+AGG | 0.546949 | 4.2:+71561516 | None:intergenic |
| CGTTGCAACGCCGACGGTAA+CGG | 0.550069 | 4.2:+71564502 | None:intergenic |
| ATTTGTTTGGATGGATACTG+AGG | 0.551888 | 4.2:-71557785 | MS.gene043157:CDS |
| TAACGGAGTTCAACGGAGAA+CGG | 0.554457 | 4.2:+71564519 | None:intergenic |
| GTAGAAAGTGAACTTGCCTC+AGG | 0.554829 | 4.2:-71557738 | MS.gene043157:CDS |
| GACATTGAAGATTTGCTCGT+CGG | 0.554841 | 4.2:-71564569 | MS.gene043157:CDS |
| CTCTGGGATGGAAGGCAAAG+TGG | 0.556380 | 4.2:-71558851 | MS.gene043157:CDS |
| GCCGCTACCGGAGCTCCACC+GGG | 0.560907 | 4.2:-71564545 | MS.gene043157:CDS |
| AACATGCTCGGGGTCACCTA+GGG | 0.562046 | 4.2:-71561532 | MS.gene043157:CDS |
| GAGATTCTTCCAATTAATGT+TGG | 0.562618 | 4.2:-71561587 | MS.gene043157:CDS |
| AAGGAAGAATCTGCACAAAA+GGG | 0.564610 | 4.2:-71557760 | MS.gene043157:CDS |
| ACCTTTGGAAGGTCAAGCGC+TGG | 0.566270 | 4.2:+71561751 | None:intergenic |
| GTTGCTGGCTGTGTCCCTCA+AGG | 0.566523 | 4.2:-71561901 | MS.gene043157:CDS |
| ACGCCATACTCTGGGATGGA+AGG | 0.569239 | 4.2:-71558859 | MS.gene043157:CDS |
| TTTGAATTCACACCTTTGGA+AGG | 0.569824 | 4.2:+71561740 | None:intergenic |
| GTAAAATCTGTGGTATCTGA+TGG | 0.573159 | 4.2:-71561284 | MS.gene043157:CDS |
| CCGTTGAACTCCGTTACCGT+CGG | 0.574263 | 4.2:-71564512 | MS.gene043157:CDS |
| AAACATGCTCGGGGTCACCT+AGG | 0.574559 | 4.2:-71561533 | MS.gene043157:CDS |
| CGAGATGTGAAAGAGCTGGA+AGG | 0.575668 | 4.2:-71561874 | MS.gene043157:CDS |
| TTAGAAATTACCATATGCTC+CGG | 0.576298 | 4.2:+71561229 | None:intergenic |
| ATTGCAAGACAAAACATGCT+CGG | 0.578759 | 4.2:-71561544 | MS.gene043157:CDS |
| TATGCTTGGAAGTTCAGCTC+TGG | 0.580362 | 4.2:-71558078 | MS.gene043157:CDS |
| ATTTGGATGTTCTCATAACC+AGG | 0.580770 | 4.2:-71564318 | MS.gene043157:intron |
| AGTGATAGTGAATATATGGC+TGG | 0.587060 | 4.2:-71564182 | MS.gene043157:CDS |
| GTCCCCAAGTCAATCTGCCA+TGG | 0.587573 | 4.2:-71561968 | MS.gene043157:CDS |
| CCAGATCACACACCTGGTCG+AGG | 0.591497 | 4.2:+71559957 | None:intergenic |
| GAGATGTGAAAGAGCTGGAA+GGG | 0.591544 | 4.2:-71561873 | MS.gene043157:CDS |
| TGAGAAGACCGACAATTCAA+GGG | 0.600489 | 4.2:-71557844 | MS.gene043157:CDS |
| CTGGCCAAGATAACATCTGT+GGG | 0.605676 | 4.2:-71558059 | MS.gene043157:CDS |
| TAATTACTGGTGCAGTTGCA+CGG | 0.605718 | 4.2:-71561995 | MS.gene043157:intron |
| AAGTGCTCAAGGATAAAAGG+TGG | 0.609787 | 4.2:+71560699 | None:intergenic |
| AGATAACATCTGTGGGAAGG+TGG | 0.611853 | 4.2:-71558052 | MS.gene043157:CDS |
| ATTCACGCCATACTCTGGGA+TGG | 0.613962 | 4.2:-71558863 | MS.gene043157:CDS |
| AGAAAGGAGCATGAGAAAGT+GGG | 0.617963 | 4.2:-71557700 | MS.gene043157:CDS |
| GCTGAAGGTTGGTCATACAA+AGG | 0.618455 | 4.2:-71558135 | MS.gene043157:intron |
| AGAGAAGACATATCCTGAGT+AGG | 0.619463 | 4.2:+71557960 | None:intergenic |
| TTGCTTTAGATCAGATCCTG+AGG | 0.623187 | 4.2:+71557722 | None:intergenic |
| AAATCCACATATTATATCAG+TGG | 0.624202 | 4.2:+71560430 | None:intergenic |
| TCGTGTTGTTGAAGTTGTAG+AGG | 0.625042 | 4.2:-71561821 | MS.gene043157:CDS |
| CCAAGATAACATCTGTGGGA+AGG | 0.625892 | 4.2:-71558055 | MS.gene043157:CDS |
| TCTGGCCAAGATAACATCTG+TGG | 0.625993 | 4.2:-71558060 | MS.gene043157:CDS |
| GCACTTGAAAGAGATAGCAG+AGG | 0.626453 | 4.2:-71560682 | MS.gene043157:CDS |
| GCCAGCGCTTGACCTTCCAA+AGG | 0.629616 | 4.2:-71561752 | MS.gene043157:intron |
| ACACAGCCAGCAACAACTAG+TGG | 0.634478 | 4.2:+71561910 | None:intergenic |
| TGGCTGAGCAGTGAAGATAC+CGG | 0.637316 | 4.2:-71561248 | MS.gene043157:CDS |
| CCTTAACTGAGCTTGTGCCA+GGG | 0.643352 | 4.2:-71560463 | MS.gene043157:CDS |
| ATGAATCGAGAATATACTGT+GGG | 0.644518 | 4.2:-71560509 | MS.gene043157:CDS |
| ATCCATGGCAGATTGACTTG+GGG | 0.656976 | 4.2:+71561966 | None:intergenic |
| AACGGAGAACGGAATCCCGG+TGG | 0.670316 | 4.2:+71564530 | None:intergenic |
| CCGACGGTAACGGAGTTCAA+CGG | 0.671119 | 4.2:+71564512 | None:intergenic |
| TGCAAGACAAAACATGCTCG+GGG | 0.687262 | 4.2:-71561542 | MS.gene043157:CDS |
| GGTTGGGCGTGTAAAATCTG+TGG | 0.710077 | 4.2:-71561294 | MS.gene043157:CDS |
| AATGAATCGAGAATATACTG+TGG | 0.719912 | 4.2:-71560510 | MS.gene043157:CDS |
| AAAATGAATAGGGACACCTG+CGG | 0.776532 | 4.2:-71558956 | MS.gene043157:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 71557599 | 71564597 | 71557599 | ID=MS.gene043157 |
| chr4.2 | mRNA | 71557599 | 71564597 | 71557599 | ID=MS.gene043157.t1;Parent=MS.gene043157 |
| chr4.2 | exon | 71564511 | 71564597 | 71564511 | ID=MS.gene043157.t1.exon1;Parent=MS.gene043157.t1 |
| chr4.2 | CDS | 71564511 | 71564597 | 71564511 | ID=cds.MS.gene043157.t1;Parent=MS.gene043157.t1 |
| chr4.2 | exon | 71564319 | 71564357 | 71564319 | ID=MS.gene043157.t1.exon2;Parent=MS.gene043157.t1 |
| chr4.2 | CDS | 71564319 | 71564357 | 71564319 | ID=cds.MS.gene043157.t1;Parent=MS.gene043157.t1 |
| chr4.2 | exon | 71564113 | 71564204 | 71564113 | ID=MS.gene043157.t1.exon3;Parent=MS.gene043157.t1 |
| chr4.2 | CDS | 71564113 | 71564204 | 71564113 | ID=cds.MS.gene043157.t1;Parent=MS.gene043157.t1 |
| chr4.2 | exon | 71561753 | 71562002 | 71561753 | ID=MS.gene043157.t1.exon4;Parent=MS.gene043157.t1 |
| chr4.2 | CDS | 71561753 | 71562002 | 71561753 | ID=cds.MS.gene043157.t1;Parent=MS.gene043157.t1 |
| chr4.2 | exon | 71561511 | 71561630 | 71561511 | ID=MS.gene043157.t1.exon5;Parent=MS.gene043157.t1 |
| chr4.2 | CDS | 71561511 | 71561630 | 71561511 | ID=cds.MS.gene043157.t1;Parent=MS.gene043157.t1 |
| chr4.2 | exon | 71561240 | 71561315 | 71561240 | ID=MS.gene043157.t1.exon6;Parent=MS.gene043157.t1 |
| chr4.2 | CDS | 71561240 | 71561315 | 71561240 | ID=cds.MS.gene043157.t1;Parent=MS.gene043157.t1 |
| chr4.2 | exon | 71560611 | 71560819 | 71560611 | ID=MS.gene043157.t1.exon7;Parent=MS.gene043157.t1 |
| chr4.2 | CDS | 71560611 | 71560819 | 71560611 | ID=cds.MS.gene043157.t1;Parent=MS.gene043157.t1 |
| chr4.2 | exon | 71560426 | 71560534 | 71560426 | ID=MS.gene043157.t1.exon8;Parent=MS.gene043157.t1 |
| chr4.2 | CDS | 71560426 | 71560534 | 71560426 | ID=cds.MS.gene043157.t1;Parent=MS.gene043157.t1 |
| chr4.2 | exon | 71559970 | 71560062 | 71559970 | ID=MS.gene043157.t1.exon9;Parent=MS.gene043157.t1 |
| chr4.2 | CDS | 71559970 | 71560062 | 71559970 | ID=cds.MS.gene043157.t1;Parent=MS.gene043157.t1 |
| chr4.2 | exon | 71558807 | 71558967 | 71558807 | ID=MS.gene043157.t1.exon10;Parent=MS.gene043157.t1 |
| chr4.2 | CDS | 71558807 | 71558967 | 71558807 | ID=cds.MS.gene043157.t1;Parent=MS.gene043157.t1 |
| chr4.2 | exon | 71557599 | 71558150 | 71557599 | ID=MS.gene043157.t1.exon11;Parent=MS.gene043157.t1 |
| chr4.2 | CDS | 71557599 | 71558150 | 71557599 | ID=cds.MS.gene043157.t1;Parent=MS.gene043157.t1 |
| Gene Sequence |
| Protein sequence |
