AlfalfaGEDB | MS.gene043977

M. sativa cultivar XinJiangDaYe / MS.gene043977

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043977.t1	XP_003589268.3	94.1	237	12	2	1	236	170	405	1.40E-121	445.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043977.t1	Q8RYD1	47.5	120	60	3	2	119	39	157	2.8e-22	107.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043977.t1	A0A396JGJ9	94.1	237	12	2	1	236	170	405	9.9e-122	445.7

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene043977.t1	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene043977	MS.gene043976	PPI
MS.gene043977	MS.gene24349	PPI
MS.gene043977	MS.gene67090	PPI
MS.gene24342	MS.gene043977	PPI
MS.gene24348	MS.gene043977	PPI
MS.gene24349	MS.gene043977	PPI
MS.gene27370	MS.gene043977	PPI
MS.gene67073	MS.gene043977	PPI
MS.gene67075	MS.gene043977	PPI
MS.gene67076	MS.gene043977	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043977.t1	MTR_1g021290	93.820	178	9	2	1	177	170	346	2.56e-112	326
MS.gene043977.t1	MTR_1g021290	73.529	102	26	1	1	102	42	142	3.63e-46	157
MS.gene043977.t1	MTR_1g021320	50.613	326	70	4	1	236	197	521	8.34e-90	282
MS.gene043977.t1	MTR_1g021320	71.074	121	34	1	1	121	40	159	5.47e-50	174
MS.gene043977.t1	MTR_1g021435	59.756	246	71	4	1	236	37	264	1.01e-86	261
MS.gene043977.t1	MTR_1g021440	51.439	278	75	5	1	236	37	296	7.81e-81	244
MS.gene043977.t1	MTR_1g021400	57.371	251	68	6	1	236	45	271	1.08e-79	243
MS.gene043977.t1	MTR_1g021440	51.439	278	75	5	1	236	37	296	3.60e-79	243
MS.gene043977.t1	MTR_1g021410	55.422	249	77	5	1	236	37	264	6.35e-77	236
MS.gene043977.t1	MTR_1g021440	51.504	266	75	5	1	236	37	278	1.95e-76	233

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043977.t1	AT4G33280	51.961	102	47	2	1	101	38	138	4.53e-29	110
MS.gene043977.t1	AT4G33280	45.455	121	63	3	1	119	38	157	1.23e-28	110
MS.gene043977.t1	AT4G33280	51.961	102	47	2	1	101	38	138	2.03e-28	111
MS.gene043977.t1	AT4G34400	36.905	84	52	1	1	84	30	112	3.61e-13	68.6
MS.gene043977.t1	AT4G34400	36.905	84	52	1	1	84	30	112	3.61e-13	68.6

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Find 51 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTCAACAGCACTTGTTG+AGG	0.643971	1.3:-3411508	MS.gene043977:CDS
AAATGAGGAGGAAAATGATT+GGG	0.529211	1.3:+3411476	None:intergenic
AACAAAATATGGAAATGAGG+AGG	0.576763	1.3:+3411464	None:intergenic
AACTCCACTAGGACCTTTAA+GGG	0.495173	1.3:+3412980	None:intergenic
AAGCTCCGCTGAAAATACAA+TGG	0.674487	1.3:+3411538	None:intergenic
AAGGTCCTAGTGGAGTTACA+TGG	0.559188	1.3:-3412974	MS.gene043977:CDS
ACGAGAACCACTGACATTGA+AGG	0.493660	1.3:+3411428	None:intergenic
ACTGAATAGCTGCGGTCTTA+GGG	0.455599	1.3:+3412637	None:intergenic
ACTTGTAACAAGCAACACCC+AGG	0.683080	1.3:-3412525	MS.gene043977:intron
AGATGGCACTGTTTACTTCG+TGG	0.564148	1.3:-3412928	MS.gene043977:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATGAGGAGGAAAATGATT+GGG	+	chr1.3:3412984-3413003	None:intergenic	30.0%
AACAAAATATGGAAATGAGG+AGG	+	chr1.3:3412996-3413015	None:intergenic	30.0%
AAGAGAAGTAGTATTATCGA+TGG	+	chr1.3:3412524-3412543	None:intergenic	30.0%
AGACAGTTTAATAGCCATTA+AGG	+	chr1.3:3412561-3412580	None:intergenic	30.0%
GACAGTTTAATAGCCATTAA+GGG	+	chr1.3:3412560-3412579	None:intergenic	30.0%
GAGTTACATGGAAGAATATA+AGG	-	chr1.3:3411495-3411514	MS.gene043977:CDS	30.0%
GATGTTCATACATGATGTAT+CGG	-	chr1.3:3412182-3412201	MS.gene043977:intron	30.0%
TAAGCTCTGTTATGAATATC+TGG	+	chr1.3:3412819-3412838	None:intergenic	30.0%
TTATGTCATCCTTCACAAAT+AGG	+	chr1.3:3412430-3412449	None:intergenic	30.0%
TTTAATAGCCATTAAGGGAA+AGG	+	chr1.3:3412555-3412574	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	3411422	3413057	3411422	ID=MS.gene043977
chr1.3	mRNA	3411422	3413057	3411422	ID=MS.gene043977.t1;Parent=MS.gene043977
chr1.3	exon	3412526	3413057	3412526	ID=MS.gene043977.t1.exon1;Parent=MS.gene043977.t1
chr1.3	CDS	3412526	3413057	3412526	ID=cds.MS.gene043977.t1;Parent=MS.gene043977.t1
chr1.3	exon	3411422	3411597	3411422	ID=MS.gene043977.t1.exon2;Parent=MS.gene043977.t1
chr1.3	CDS	3411422	3411597	3411422	ID=cds.MS.gene043977.t1;Parent=MS.gene043977.t1

Gene Sequence

>MS.gene043977

GCACTTCCTAAAACATTTTCAGACAATTTAAATAAGAAGTTGCCAGAAAATGTGACCCTTAAAGGTCCTAGTGGAGTTACATGGAAGAATATAAGGCTGACAACTAGAGATGGCACTGTTTACTTCGTGGACGGTTGGCAACAATTCATGAACGATCACTCGTTGAAAGCTAATGATTTCCTTGTCTGTAAGTACAATGGTGAATCGCACTTTGAGGTTTTAATCTTTGATGGAGAAAGCTTCTGCGAGAAAGAAGCTTCTTATTTTGTCGAAAAATGCGGCCATGCTCAAACAGCACAAGGGGGTAGCAATGCATCGCAAACAAACAATTCTGTTGAAGAAGTCAATACTGCTTCTAATGCTGGTGATTCCCCTGAGCAGTTCACGGATGATGCAGTCCCTAAGACCGCAGCTATTCAGTGTCCCTTCCTACCAACCGGCAAAAGAACCAAGAGGAGAAGAAGATCACCAAAAGCTGCTAATTGGGGTGCGAGAGCACATGCTTGGGTGACTTGTAACAAGCAACACCCAGGTTAAAATATTTCTTTCTTTTCCATTAACTTTTACGATATTAAATGGAACCCAGTCAATACGAATATCTAGTGTGTATCTATGTTGACTATGATAAATTCTGATTTATATTCATATTGAATCACTAGCTAAACTTAAAGTAGCTAACCATTTTTTTTTTGGTTAACTTTGTATAACCAACCACTTTTCTTTTTTCATTTGATTTATGACTTGAATCATGTATTATTTTGATGTTCATACATGATGTATCGGCTTATAGTTTTGTTGTTATGTCACATGCTTTTACTTAAAAATGACATATCGGCCTAGTTCAAATAAATCAATTTTTCACTACCTCTATATATATTCCTCTCTCCCATGTGTGTTTCTCTCCCTATCTCTAGCTAACTTGCCAACTAAGCACTTTTATCTCGAGTGTTGTCAAATATCGGCCATGGCGGATTGTGGTGGTGGATTTTTTAACAACCGGCATGACCTATTTGTGAAGGATGACATAACCATGGTGTTTTGTGGTGGATATGGCGGCGCCTATGGGGATTTTTGGCTTTCCGTCACGGTCTGCCATCCGCCATCGATAATACTACTTCTCTTCATTTTGGCCTTTCCCTTAATGGCTATTAAACTGTCTCTCAGTCAAGTAATCTCTGGTAACCTTAATATCATCAACATAAACAAGAACTACAATTAGTTTTCCTCCTTGTGAATGTTTGAACTAAAGATTTTGATTTCCTTGACTTTTCTTATAGCCCAAGCACACCACTGCATTTGATTTCATTCTGGTTCTCAAAAATTTGTATTATAACTCATCTGATCTTCCCTAGCAAACTGATACTTGTCTAGGAAAAAGTATTTTGCATTCAAATCCAGATATTCATAACAGAGCTTATCCTTTTATTTGTAACTCATGATTGAACTGTTCAATTCCTCAGAGGCAGCCAGCCCGAATAGAAGTGGGAAGGTAGACGACCATTGTATTTTCAGCGGAGCTTCACTGTCAAAGTCAACAGCACTTGTTGAGGAAAAGATAGCCCAATCATTTTCCTCCTCATTTCCATATTTTGTTAAAATAATTAAAGCCTTCAATGTCAGTGGTTCTCGTACTATT

Protein sequence

>MS.gene043977.t1

ALPKTFSDNLNKKLPENVTLKGPSGVTWKNIRLTTRDGTVYFVDGWQQFMNDHSLKANDFLVCKYNGESHFEVLIFDGESFCEKEASYFVEKCGHAQTAQGGSNASQTNNSVEEVNTASNAGDSPEQFTDDAVPKTAAIQCPFLPTGKRTKRRRRSPKAANWGARAHAWVTCNKQHPEAASPNRSGKVDDHCIFSGASLSKSTALVEEKIAQSFSSSFPYFVKIIKAFNVSGSRTI