AlfalfaGEDB | MS.gene67075

M. sativa cultivar XinJiangDaYe / MS.gene67075

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67075.t1	XP_003589270.2	95.1	204	8	2	1	202	197	400	2.40E-106	394.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67075.t1	Q8RYD1	45.4	119	63	2	2	118	39	157	5.7e-24	112.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67075.t1	G7I4K4	95.1	204	8	2	1	202	197	400	1.7e-106	394.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene67075.t1	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene27370	MS.gene67075	PPI
MS.gene67075	MS.gene67090	PPI
MS.gene67153	MS.gene67075	PPI
MS.gene67075	MS.gene043977	PPI
MS.gene67073	MS.gene67075	PPI
MS.gene67075	MS.gene24349	PPI
MS.gene67075	MS.gene24342	PPI
MS.gene67075	MS.gene043976	PPI
MS.gene67076	MS.gene67075	PPI
MS.gene24348	MS.gene67075	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67075.t1	MTR_1g021320	95.098	204	8	2	1	202	197	400	2.31e-134	397
MS.gene67075.t1	MTR_1g021320	72.868	129	35	0	1	129	40	168	1.82e-59	199
MS.gene67075.t1	MTR_1g021440	66.316	190	53	2	1	188	37	217	8.41e-85	252
MS.gene67075.t1	MTR_1g021440	66.316	190	53	2	1	188	37	217	1.05e-84	253
MS.gene67075.t1	MTR_1g021440	66.316	190	53	2	1	188	37	217	2.96e-83	252
MS.gene67075.t1	MTR_1g021440	66.316	190	53	2	1	188	37	217	5.16e-83	251
MS.gene67075.t1	MTR_1g021350	62.371	194	52	2	1	194	36	208	2.62e-78	238
MS.gene67075.t1	MTR_1g021330	64.706	187	56	3	1	187	41	217	3.59e-76	234
MS.gene67075.t1	MTR_1g021435	60.638	188	40	1	1	188	37	190	3.16e-71	221
MS.gene67075.t1	MTR_1g021290	62.136	206	38	2	1	203	170	338	3.08e-70	217
MS.gene67075.t1	MTR_1g021290	71.287	101	29	0	1	101	42	142	8.51e-47	157
MS.gene67075.t1	MTR_1g021400	65.644	163	49	2	1	163	45	200	1.16e-64	204
MS.gene67075.t1	MTR_1g021410	62.805	164	54	2	1	163	37	194	6.58e-61	194
MS.gene67075.t1	MTR_1g021360	72.868	129	35	0	1	129	40	168	2.46e-59	193
MS.gene67075.t1	MTR_1g021360	53.968	189	31	2	1	189	197	329	3.68e-58	190
MS.gene67075.t1	MTR_1g021380	54.255	188	37	3	1	188	36	174	1.79e-56	182
MS.gene67075.t1	MTR_1g021500	47.556	225	78	6	1	203	41	247	1.53e-53	177
MS.gene67075.t1	MTR_1g021500	47.556	225	78	6	1	203	41	247	3.44e-53	177
MS.gene67075.t1	MTR_1g021270	47.143	70	36	1	18	87	47	115	5.36e-16	75.9
MS.gene67075.t1	MTR_7g050720	32.450	151	85	3	2	136	26	175	5.98e-15	72.4
MS.gene67075.t1	MTR_7g050640	34.177	79	52	0	1	79	28	106	1.60e-13	64.7
MS.gene67075.t1	MTR_7g050710	34.884	86	55	1	2	87	29	113	1.65e-13	68.2
MS.gene67075.t1	MTR_7g050580	37.333	75	47	0	2	76	29	103	1.98e-13	67.8
MS.gene67075.t1	MTR_7g050590	35.443	79	51	0	2	80	2	80	3.78e-13	67.0
MS.gene67075.t1	MTR_3g098790	29.371	143	80	3	1	131	28	161	1.48e-12	65.5
MS.gene67075.t1	MTR_1g034240	40.000	75	45	0	2	76	23	97	1.06e-11	63.5
MS.gene67075.t1	MTR_5g044670	41.333	75	44	0	2	76	32	106	2.55e-11	62.0
MS.gene67075.t1	MTR_1g034210	40.541	74	44	0	2	75	23	96	3.81e-11	61.6
MS.gene67075.t1	MTR_1g034210	40.541	74	44	0	2	75	23	96	4.39e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67075.t1	AT4G33280	45.000	120	64	2	1	118	38	157	7.38e-30	112
MS.gene67075.t1	AT4G33280	49.505	101	50	1	1	100	38	138	1.43e-29	111
MS.gene67075.t1	AT4G33280	49.505	101	50	1	1	100	38	138	5.80e-29	111
MS.gene67075.t1	AT4G34400	42.169	83	48	0	1	83	30	112	8.76e-19	83.6
MS.gene67075.t1	AT4G34400	42.169	83	48	0	1	83	30	112	8.76e-19	83.6
MS.gene67075.t1	AT3G06220	34.146	82	53	1	2	83	25	105	1.26e-15	71.6
MS.gene67075.t1	AT3G06160	34.667	75	49	0	1	75	39	113	1.90e-12	65.1
MS.gene67075.t1	AT3G06160	34.667	75	49	0	1	75	24	98	2.38e-12	65.1
MS.gene67075.t1	AT3G06160	34.667	75	49	0	1	75	24	98	2.38e-12	65.1
MS.gene67075.t1	AT3G06160	34.667	75	49	0	1	75	24	98	2.38e-12	65.1
MS.gene67075.t1	AT3G06160	34.667	75	49	0	1	75	83	157	2.43e-12	65.1
MS.gene67075.t1	AT5G60130	40.909	88	47	2	1	83	28	115	5.37e-12	63.2
MS.gene67075.t1	AT5G60130	40.909	88	47	2	1	83	28	115	6.72e-12	63.5
MS.gene67075.t1	AT5G60130	40.909	88	47	2	1	83	2	89	7.41e-12	63.5
MS.gene67075.t1	AT3G18990	37.333	75	47	0	2	76	22	96	7.47e-12	63.5
MS.gene67075.t1	AT5G66980	40.449	89	48	2	1	85	24	111	7.61e-12	63.5
MS.gene67075.t1	AT3G18990	37.333	75	47	0	2	76	22	96	8.35e-12	63.5
MS.gene67075.t1	AT5G60130	40.909	88	47	2	1	83	25	112	1.04e-11	63.2
MS.gene67075.t1	AT5G60130	40.909	88	47	2	1	83	28	115	1.12e-11	63.2
MS.gene67075.t1	AT3G53310	36.000	75	48	0	2	76	26	100	8.41e-11	60.5

Find 34 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACACCACCGGGACCTTTA+AGG	0.159160	1.2:+3453038	None:intergenic
CCAGAAAATGTGACCCTTAA+AGG	0.236805	1.2:-3453052	MS.gene67075:CDS
GGTGGTGTTGCATGGAAAAT+AGG	0.237795	1.2:-3453025	MS.gene67075:CDS
TGGTTTATATGAGAAATTTC+AGG	0.307206	1.2:-3452723	MS.gene67075:CDS
CCTACCAACCAACCGGAAAA+AGG	0.307983	1.2:-3452562	MS.gene67075:CDS
TTTCAGGATCTTAACAATAT+AGG	0.337128	1.2:-3452707	MS.gene67075:CDS
CCCCTAGTTCAGTTTGAGTA+TGG	0.362232	1.2:+3452816	None:intergenic
CGACATCAGCTAATTGTTGT+TGG	0.381642	1.2:-3452523	MS.gene67075:CDS
GTGGTGTTGCATGGAAAATA+GGG	0.415820	1.2:-3453024	MS.gene67075:CDS
AACACCACCGGGACCTTTAA+GGG	0.419570	1.2:+3453039	None:intergenic
GTATCAGCTATGAACTGCTC+AGG	0.438283	1.2:+3452614	None:intergenic
GTTGTTGGAGAGCACTTGAC+TGG	0.446323	1.2:-3452508	MS.gene67075:CDS
CCAACCGTCCACGAAGTAAA+CGG	0.457669	1.2:+3452982	None:intergenic
GAAGTCAATACTGCTTCTAA+TGG	0.478633	1.2:-3452758	MS.gene67075:CDS
GTTTCAACAGGTACAGCTAA+TGG	0.493162	1.2:+3452680	None:intergenic
TTCCTTGTCTTTAATTACAA+TGG	0.493431	1.2:-3452920	MS.gene67075:CDS
CCGTTTACTTCGTGGACGGT+TGG	0.508441	1.2:-3452982	MS.gene67075:CDS
GCCATACTCAAACTGAACTA+GGG	0.513331	1.2:-3452817	MS.gene67075:CDS
GGCCATACTCAAACTGAACT+AGG	0.514000	1.2:-3452818	MS.gene67075:CDS
AGACGATACCGTTTACTTCG+TGG	0.517967	1.2:-3452990	MS.gene67075:CDS
CAGTTTCCCTACCAACCAAC+CGG	0.519266	1.2:-3452569	MS.gene67075:CDS
CACCATTGTAATTAAAGACA+AGG	0.542064	1.2:+3452918	None:intergenic
TATCAGCTATGAACTGCTCA+GGG	0.548226	1.2:+3452615	None:intergenic
AAGGTCCCGGTGGTGTTGCA+TGG	0.549921	1.2:-3453033	MS.gene67075:CDS
TCTAATGGTGGTGTTGAATG+TGG	0.555737	1.2:-3452743	MS.gene67075:CDS
AATGTGACCCTTAAAGGTCC+CGG	0.577654	1.2:-3453046	MS.gene67075:CDS
TTGTTGGAGAGCACTTGACT+GGG	0.586591	1.2:-3452507	None:intergenic
GTCAATACTGCTTCTAATGG+TGG	0.593959	1.2:-3452755	MS.gene67075:CDS
CCATACTCAAACTGAACTAG+GGG	0.634393	1.2:-3452816	MS.gene67075:CDS
GTAGGGAAACTGTATAGCTG+TGG	0.649466	1.2:+3452580	None:intergenic
GATACCGTTTACTTCGTGGA+CGG	0.682240	1.2:-3452986	MS.gene67075:CDS
GTGACCCTTAAAGGTCCCGG+TGG	0.715661	1.2:-3453043	MS.gene67075:CDS
ATCAGCTATGAACTGCTCAG+GGG	0.739451	1.2:+3452616	None:intergenic
CATACTCAAACTGAACTAGG+GGG	0.777970	1.2:-3452815	MS.gene67075:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTGTCTGAAAATGTTTT+AGG	+	chr1.2:3452517-3452536	None:intergenic	20.0%
!	TGGTTTATATGAGAAATTTC+AGG	-	chr1.2:3452879-3452898	MS.gene67075:CDS	25.0%
!	TTCCTTGTCTTTAATTACAA+TGG	-	chr1.2:3452682-3452701	MS.gene67075:CDS	25.0%
!	TTTCAGGATCTTAACAATAT+AGG	-	chr1.2:3452895-3452914	MS.gene67075:CDS	25.0%
!!	AATGAAAAGACTTTTAATGC+TGG	-	chr1.2:3452949-3452968	MS.gene67075:CDS	25.0%
!!	AGCATTAAAAGTCTTTTCAT+TGG	+	chr1.2:3452950-3452969	None:intergenic	25.0%
!!!	AAAGAAGCTTCTTATTTTGT+CGG	-	chr1.2:3452754-3452773	MS.gene67075:CDS	25.0%
!!!	TTTGATGTTTTGATCTTTGA+TGG	-	chr1.2:3452715-3452734	MS.gene67075:CDS	25.0%
	CACCATTGTAATTAAAGACA+AGG	+	chr1.2:3452687-3452706	None:intergenic	30.0%
!!	TTTTCATTGGTAGTTTCAAC+AGG	+	chr1.2:3452937-3452956	None:intergenic	30.0%
!!!	TCTTATTTTGTCGGAAAATG+CGG	-	chr1.2:3452763-3452782	MS.gene67075:CDS	30.0%
	GAAGTCAATACTGCTTCTAA+TGG	-	chr1.2:3452844-3452863	MS.gene67075:CDS	35.0%
	CATACTCAAACTGAACTAGG+GGG	-	chr1.2:3452787-3452806	MS.gene67075:CDS	40.0%
	CCAGAAAATGTGACCCTTAA+AGG	-	chr1.2:3452550-3452569	MS.gene67075:CDS	40.0%
	CCATACTCAAACTGAACTAG+GGG	-	chr1.2:3452786-3452805	MS.gene67075:CDS	40.0%
	CGACATCAGCTAATTGTTGT+TGG	-	chr1.2:3453079-3453098	MS.gene67075:CDS	40.0%
	GCCATACTCAAACTGAACTA+GGG	-	chr1.2:3452785-3452804	MS.gene67075:CDS	40.0%
	GTCAATACTGCTTCTAATGG+TGG	-	chr1.2:3452847-3452866	MS.gene67075:CDS	40.0%
	GTTTCAACAGGTACAGCTAA+TGG	+	chr1.2:3452925-3452944	None:intergenic	40.0%
	TATCAGCTATGAACTGCTCA+GGG	+	chr1.2:3452990-3453009	None:intergenic	40.0%
!	TATTTTCCATGCAACACCAC+CGG	+	chr1.2:3452578-3452597	None:intergenic	40.0%
!	TCTAATGGTGGTGTTGAATG+TGG	-	chr1.2:3452859-3452878	MS.gene67075:CDS	40.0%
!	TCTTCTCTTGCTCCTTTTTC+CGG	+	chr1.2:3453055-3453074	None:intergenic	40.0%
!!	CCTTTAAGGGTCACATTTTC+TGG	+	chr1.2:3452553-3452572	None:intergenic	40.0%
!!	GTGGTGTTGCATGGAAAATA+GGG	-	chr1.2:3452578-3452597	MS.gene67075:CDS	40.0%
	AATGTGACCCTTAAAGGTCC+CGG	-	chr1.2:3452556-3452575	MS.gene67075:CDS	45.0%
	AGACGATACCGTTTACTTCG+TGG	-	chr1.2:3452612-3452631	MS.gene67075:CDS	45.0%
	ATCAGCTATGAACTGCTCAG+GGG	+	chr1.2:3452989-3453008	None:intergenic	45.0%
	CCCCTAGTTCAGTTTGAGTA+TGG	+	chr1.2:3452789-3452808	None:intergenic	45.0%
	GATACCGTTTACTTCGTGGA+CGG	-	chr1.2:3452616-3452635	MS.gene67075:CDS	45.0%
	GGCCATACTCAAACTGAACT+AGG	-	chr1.2:3452784-3452803	MS.gene67075:CDS	45.0%
	GTAGGGAAACTGTATAGCTG+TGG	+	chr1.2:3453025-3453044	None:intergenic	45.0%
	GTATCAGCTATGAACTGCTC+AGG	+	chr1.2:3452991-3453010	None:intergenic	45.0%
!	ATTTTCCATGCAACACCACC+GGG	+	chr1.2:3452577-3452596	None:intergenic	45.0%
!!	CTTTTTCCGGTTGGTTGGTA+GGG	+	chr1.2:3453042-3453061	None:intergenic	45.0%
!!	GGTGGTGTTGCATGGAAAAT+AGG	-	chr1.2:3452577-3452596	MS.gene67075:CDS	45.0%
!!	TTGTTGGAGAGCACTTGACT+GGG	-	chr1.2:3453095-3453114	MS.gene67075:CDS	45.0%
	AACACCACCGGGACCTTTAA+GGG	+	chr1.2:3452566-3452585	None:intergenic	50.0%
	ACTAGGGGGTAGCAAAGCAA+AGG	-	chr1.2:3452801-3452820	MS.gene67075:CDS	50.0%
	CAGTTTCCCTACCAACCAAC+CGG	-	chr1.2:3453033-3453052	MS.gene67075:CDS	50.0%
	CCAACCGTCCACGAAGTAAA+CGG	+	chr1.2:3452623-3452642	None:intergenic	50.0%
	CCTACCAACCAACCGGAAAA+AGG	-	chr1.2:3453040-3453059	MS.gene67075:CDS	50.0%
!	CTCTTGCTCCTTTTTCCGGT+TGG	+	chr1.2:3453051-3453070	None:intergenic	50.0%
!	TGCTCCTTTTTCCGGTTGGT+TGG	+	chr1.2:3453047-3453066	None:intergenic	50.0%
!!	CCTTTTTCCGGTTGGTTGGT+AGG	+	chr1.2:3453043-3453062	None:intergenic	50.0%
!!	GTTGTTGGAGAGCACTTGAC+TGG	-	chr1.2:3453094-3453113	MS.gene67075:CDS	50.0%
	CAACACCACCGGGACCTTTA+AGG	+	chr1.2:3452567-3452586	None:intergenic	55.0%
	CCGTTTACTTCGTGGACGGT+TGG	-	chr1.2:3452620-3452639	MS.gene67075:CDS	55.0%
	GTGACCCTTAAAGGTCCCGG+TGG	-	chr1.2:3452559-3452578	MS.gene67075:CDS	60.0%
!!	AAGGTCCCGGTGGTGTTGCA+TGG	-	chr1.2:3452569-3452588	MS.gene67075:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	3452508	3453116	3452508	ID=MS.gene67075
chr1.2	mRNA	3452508	3453116	3452508	ID=MS.gene67075.t1;Parent=MS.gene67075
chr1.2	exon	3452508	3453116	3452508	ID=MS.gene67075.t1.exon1;Parent=MS.gene67075.t1
chr1.2	CDS	3452508	3453116	3452508	ID=cds.MS.gene67075.t1;Parent=MS.gene67075.t1

Gene Sequence

>MS.gene67075

GCACTTCCTAAAACATTTTCAGACAATTTAAATAAGAAGTTGCCAGAAAATGTGACCCTTAAAGGTCCCGGTGGTGTTGCATGGAAAATAGGGCTCATAACTAGAGACGATACCGTTTACTTCGTGGACGGTTGGCAACGATTCGTGAACGATCACTCTTTGAAAGATAATGATTTCCTTGTCTTTAATTACAATGGTGAATCACACTTTGATGTTTTGATCTTTGATGGAGAAAGCTTCTGTGAGAAAGAAGCTTCTTATTTTGTCGGAAAATGCGGCCATACTCAAACTGAACTAGGGGGTAGCAAAGCAAAGGAAACAAACAATTCTATTGAAGAAGTCAATACTGCTTCTAATGGTGGTGTTGAATGTGGTTTATATGAGAAATTTCAGGATCTTAACAATATAGGAACACCATTAGCTGTACCTGTTGAAACTACCAATGAAAAGACTTTTAATGCTGGTGTCAAATCTGATTCCCCTGAGCAGTTCATAGCTGATACAGTCACTAAGACCACAGCTATACAGTTTCCCTACCAACCAACCGGAAAAAGGAGCAAGAGAAGATCATCGACATCAGCTAATTGTTGTTGGAGAGCACTTGACTGG

Protein sequence

>MS.gene67075.t1

ALPKTFSDNLNKKLPENVTLKGPGGVAWKIGLITRDDTVYFVDGWQRFVNDHSLKDNDFLVFNYNGESHFDVLIFDGESFCEKEASYFVGKCGHTQTELGGSKAKETNNSIEEVNTASNGGVECGLYEKFQDLNNIGTPLAVPVETTNEKTFNAGVKSDSPEQFIADTVTKTTAIQFPYQPTGKRSKRRSSTSANCCWRALDW