AlfalfaGEDB | MS.gene27370

M. sativa cultivar XinJiangDaYe / MS.gene27370

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27370.t1	XP_024636626.1	95.9	221	9	0	1	221	36	256	3.10E-115	424.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27370.t1	Q8RYD1	56.6	99	40	2	2	99	39	135	1.6e-24	114.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27370.t1	A0A396JGK5	95.9	221	9	0	1	221	36	256	2.2e-115	424.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene27370.t1	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050277	MS.gene27370	0.833903	4.08E-56	-1.69E-46
MS.gene050303	MS.gene27370	0.801152	1.03E-48	-1.69E-46
MS.gene050815	MS.gene27370	0.802496	5.46E-49	-1.69E-46
MS.gene050987	MS.gene27370	0.806918	6.51E-50	-1.69E-46
MS.gene051243	MS.gene27370	0.81191	5.53E-51	-1.69E-46
MS.gene052286	MS.gene27370	0.821649	3.61E-53	-1.69E-46
MS.gene052665	MS.gene27370	0.803756	2.99E-49	-1.69E-46
MS.gene054537	MS.gene27370	0.803014	4.27E-49	-1.69E-46
MS.gene055512	MS.gene27370	0.801175	1.02E-48	-1.69E-46
MS.gene056580	MS.gene27370	0.801897	7.25E-49	-1.69E-46
MS.gene056760	MS.gene27370	0.807072	6.04E-50	-1.69E-46
MS.gene057057	MS.gene27370	0.82909	6.25E-55	-1.69E-46
MS.gene057140	MS.gene27370	0.831012	2.12E-55	-1.69E-46
MS.gene057234	MS.gene27370	0.823815	1.13E-53	-1.69E-46
MS.gene05795	MS.gene27370	0.816316	5.89E-52	-1.69E-46
MS.gene058055	MS.gene27370	0.819279	1.26E-52	-1.69E-46
MS.gene058906	MS.gene27370	0.803004	4.29E-49	-1.69E-46
MS.gene060297	MS.gene27370	0.811106	8.26E-51	-1.69E-46
MS.gene060470	MS.gene27370	0.802288	6.02E-49	-1.69E-46
MS.gene060619	MS.gene27370	0.802335	5.89E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene27370	MS.gene67075	PPI
MS.gene67073	MS.gene27370	PPI
MS.gene27370	MS.gene043976	PPI
MS.gene24348	MS.gene27370	PPI
MS.gene67076	MS.gene27370	PPI
MS.gene27370	MS.gene043977	PPI
MS.gene67153	MS.gene27370	PPI
MS.gene27370	MS.gene67090	PPI
MS.gene27370	MS.gene24349	PPI
MS.gene27370	MS.gene24342	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27370.t1	MTR_1g021440	65.179	224	69	3	1	221	37	254	3.16e-96	283
MS.gene27370.t1	MTR_1g021440	65.179	224	69	3	1	221	37	254	1.35e-94	282
MS.gene27370.t1	MTR_1g021320	60.300	267	59	4	1	221	197	462	1.48e-94	294
MS.gene27370.t1	MTR_1g021320	76.613	124	29	0	1	124	40	163	3.77e-61	205
MS.gene27370.t1	MTR_1g021440	67.647	204	58	2	1	201	37	235	1.10e-93	276
MS.gene27370.t1	MTR_1g021440	67.647	204	58	2	1	201	37	235	3.95e-92	275
MS.gene27370.t1	MTR_1g021350	61.674	227	59	6	1	221	36	240	5.70e-85	256
MS.gene27370.t1	MTR_1g021330	62.162	222	55	4	1	221	41	234	8.88e-84	254
MS.gene27370.t1	MTR_1g021435	60.444	225	46	5	1	221	37	222	4.37e-78	239
MS.gene27370.t1	MTR_1g021380	57.143	224	49	5	1	221	36	215	5.31e-74	228
MS.gene27370.t1	MTR_1g021400	57.658	222	56	3	1	221	45	229	1.06e-72	225
MS.gene27370.t1	MTR_1g021410	56.696	224	56	5	1	221	37	222	2.26e-67	211
MS.gene27370.t1	MTR_1g021500	52.423	227	76	6	1	214	41	248	2.42e-62	201
MS.gene27370.t1	MTR_1g021500	52.423	227	76	6	1	214	41	248	6.23e-62	201
MS.gene27370.t1	MTR_1g021360	54.412	204	41	4	1	203	197	349	2.13e-60	196
MS.gene27370.t1	MTR_1g021360	73.600	125	33	0	1	125	40	164	5.32e-60	195
MS.gene27370.t1	MTR_1g021290	68.595	121	38	0	1	121	170	290	7.44e-52	171
MS.gene27370.t1	MTR_1g021290	73.267	101	27	0	1	101	42	142	2.91e-48	162
MS.gene27370.t1	MTR_1g021270	38.372	86	52	1	2	87	31	115	3.22e-14	71.2
MS.gene27370.t1	MTR_7g050710	37.838	74	46	0	2	75	29	102	4.67e-12	64.3
MS.gene27370.t1	MTR_3g098790	37.500	80	50	0	1	80	28	107	6.41e-12	64.3
MS.gene27370.t1	MTR_7g050640	37.333	75	47	0	1	75	28	102	1.02e-11	60.5
MS.gene27370.t1	MTR_7g050580	37.975	79	49	0	2	80	29	107	1.13e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27370.t1	AT4G33280	56.250	96	41	1	1	95	38	133	6.90e-31	115
MS.gene27370.t1	AT4G33280	56.250	96	41	1	1	95	38	133	1.62e-30	115
MS.gene27370.t1	AT4G33280	56.250	96	41	1	1	95	38	133	3.24e-30	115
MS.gene27370.t1	AT4G34400	39.759	83	50	0	1	83	30	112	2.84e-17	79.7
MS.gene27370.t1	AT4G34400	39.759	83	50	0	1	83	30	112	2.84e-17	79.7
MS.gene27370.t1	AT3G06220	33.784	74	49	0	2	75	25	98	9.98e-13	64.3
MS.gene27370.t1	AT3G18990	34.694	98	55	1	2	90	22	119	1.02e-12	66.6
MS.gene27370.t1	AT3G18990	34.694	98	55	1	2	90	22	119	1.02e-12	66.6
MS.gene27370.t1	AT3G06160	36.000	75	48	0	1	75	39	113	8.69e-12	63.9
MS.gene27370.t1	AT3G06160	36.000	75	48	0	1	75	24	98	1.05e-11	63.5
MS.gene27370.t1	AT3G06160	36.000	75	48	0	1	75	24	98	1.05e-11	63.5
MS.gene27370.t1	AT3G06160	36.000	75	48	0	1	75	24	98	1.05e-11	63.5
MS.gene27370.t1	AT3G06160	36.000	75	48	0	1	75	83	157	1.06e-11	63.9
MS.gene27370.t1	AT5G60130	38.636	88	49	2	1	83	28	115	1.29e-11	62.4
MS.gene27370.t1	AT5G60130	38.636	88	49	2	1	83	2	89	2.24e-11	62.4
MS.gene27370.t1	AT5G66980	40.506	79	43	1	1	75	24	102	2.39e-11	62.4
MS.gene27370.t1	AT5G60130	38.636	88	49	2	1	83	28	115	2.99e-11	62.0
MS.gene27370.t1	AT5G60130	38.636	88	49	2	1	83	28	115	3.23e-11	62.0
MS.gene27370.t1	AT5G60130	38.636	88	49	2	1	83	25	112	3.26e-11	62.0

Find 46 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACTCCACTAGGACCTTTA+AGG	0.183323	1.2:+3139559	None:intergenic
CCTGAAAATGTGAACCTTAA+AGG	0.211774	1.2:-3139573	MS.gene27370:CDS
GCTGGTCTTCCCCGCTTCTT+AGG	0.270434	1.2:+3139039	None:intergenic
AGTGGAGTTGTGTGGAATAT+AGG	0.300231	1.2:-3139546	MS.gene27370:CDS
AACTTCGTTAACAGGCTTCT+TGG	0.341598	1.2:+3139074	None:intergenic
CCAGGCGTAACTTCGTTAAC+AGG	0.352395	1.2:+3139066	None:intergenic
GTCTTCCCCGCTTCTTAGGC+TGG	0.353235	1.2:+3139043	None:intergenic
AATGCGGTTCTGAGAAATTT+CGG	0.363117	1.2:-3139248	MS.gene27370:CDS
CCCCGCTTCTTAGGCTGGCC+AGG	0.381562	1.2:+3139048	None:intergenic
CCAGCAAAATCAGCTACTTC+TGG	0.385623	1.2:-3139021	MS.gene27370:CDS
GTGGAGTTGTGTGGAATATA+GGG	0.421556	1.2:-3139545	MS.gene27370:CDS
TTTGTTAGAAAATGTGGAAA+AGG	0.421612	1.2:-3139354	MS.gene27370:CDS
GCATCAGCCATGACCTGCTC+AGG	0.424246	1.2:+3139138	None:intergenic
TGAGTCTGCTTCCCCTGAGC+AGG	0.449413	1.2:-3139151	MS.gene27370:CDS
GCGAGAAAGCAATTGTTGAT+TGG	0.450466	1.2:-3138999	MS.gene27370:CDS
TGCTTCCCCTGAGCAGGTCA+TGG	0.458901	1.2:-3139145	MS.gene27370:CDS
GAAGTCAACACTGCTTCTAA+TGG	0.464985	1.2:-3139279	MS.gene27370:CDS
GCCTGGCCAGCCTAAGAAGC+GGG	0.466869	1.2:-3139049	MS.gene27370:CDS
AGAGCCTGCTTTCTCGCATA+AGG	0.473371	1.2:+3139380	None:intergenic
CGAGAAAGCAATTGTTGATT+GGG	0.485091	1.2:-3138998	MS.gene27370:CDS
GCGGTTCCCTCCCAAACAAC+CGG	0.485847	1.2:-3139105	MS.gene27370:CDS
TCTTACTTTGTTAGAAAATG+TGG	0.486876	1.2:-3139360	MS.gene27370:CDS
TACTTCACAAACGGTTGGCA+TGG	0.494310	1.2:-3139498	MS.gene27370:CDS
GACACCGTTTACTTCACAAA+CGG	0.514012	1.2:-3139507	MS.gene27370:CDS
CCAACCGTTTGTGAAGTAAA+CGG	0.534062	1.2:+3139503	None:intergenic
CCTGTTAACGAAGTTACGCC+TGG	0.535294	1.2:-3139066	MS.gene27370:CDS
AATGAAAAGACTTCTAATGC+TGG	0.546042	1.2:-3139177	MS.gene27370:CDS
CCGTTTACTTCACAAACGGT+TGG	0.558722	1.2:-3139503	MS.gene27370:CDS
CATCAGCCATGACCTGCTCA+GGG	0.563227	1.2:+3139139	None:intergenic
CGCCTGGCCAGCCTAAGAAG+CGG	0.563693	1.2:-3139050	MS.gene27370:CDS
GAAAGCAATTGTTGATTGGG+TGG	0.568458	1.2:-3138995	MS.gene27370:CDS
ATATTCCACACAACTCCACT+AGG	0.578077	1.2:+3139549	None:intergenic
GAGACCTTATGCGAGAAAGC+AGG	0.587625	1.2:-3139384	MS.gene27370:CDS
GCAAGAGGGCGACAAGGCAA+AGG	0.587651	1.2:-3139322	MS.gene27370:CDS
GGCTGATGCAGTCACTATTG+CGG	0.596142	1.2:-3139124	MS.gene27370:CDS
TACTGAGCAAGAGGGCGACA+AGG	0.599125	1.2:-3139328	MS.gene27370:CDS
GTGAACCTTAAAGGTCCTAG+TGG	0.609317	1.2:-3139564	MS.gene27370:CDS
AAAAGGTCATACTGAGCAAG+AGG	0.615156	1.2:-3139337	MS.gene27370:CDS
AAGGTCCTAGTGGAGTTGTG+TGG	0.624686	1.2:-3139554	MS.gene27370:CDS
GCTTGTAGCAAGGAAAACTC+AGG	0.631925	1.2:-3138973	None:intergenic
CCTGGCCAGCCTAAGAAGCG+GGG	0.640983	1.2:-3139048	MS.gene27370:CDS
GTCAACACTGCTTCTAATGG+CGG	0.645750	1.2:-3139276	MS.gene27370:CDS
TGATTGGGTGGCTTGTAGCA+AGG	0.654638	1.2:-3138983	MS.gene27370:CDS
TCTAATGGCGGTGTTGAATG+CGG	0.661026	1.2:-3139264	MS.gene27370:CDS
AAAGGTCATACTGAGCAAGA+GGG	0.680883	1.2:-3139336	MS.gene27370:CDS
ATCAGCCATGACCTGCTCAG+GGG	0.726483	1.2:+3139140	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGAAGTTTTAATATTTCA+TGG	-	chr1.2:3139181-3139200	MS.gene27370:CDS	15.0%
!!!	AAATTGTCTGAAAATGTTTT+AGG	+	chr1.2:3138983-3139002	None:intergenic	20.0%
!	TCTTACTTTGTTAGAAAATG+TGG	-	chr1.2:3139229-3139248	MS.gene27370:CDS	25.0%
!	TTTGTTAGAAAATGTGGAAA+AGG	-	chr1.2:3139235-3139254	MS.gene27370:CDS	25.0%
!!	TTTTCATTGGTATTTTCGAA+AGG	+	chr1.2:3139400-3139419	None:intergenic	25.0%
	AATGAAAAGACTTCTAATGC+TGG	-	chr1.2:3139412-3139431	MS.gene27370:CDS	30.0%
!	AGCATTAGAAGTCTTTTCAT+TGG	+	chr1.2:3139413-3139432	None:intergenic	30.0%
!!!	CTCCTTTTTTTCAAGTACAA+TGG	-	chr1.2:3139148-3139167	MS.gene27370:CDS	30.0%
	CCTGAAAATGTGAACCTTAA+AGG	-	chr1.2:3139016-3139035	MS.gene27370:CDS	35.0%
	CGCCATTGTACTTGAAAAAA+AGG	+	chr1.2:3139153-3139172	None:intergenic	35.0%
!	CGAGAAAGCAATTGTTGATT+GGG	-	chr1.2:3139591-3139610	MS.gene27370:CDS	35.0%
!!	AATGCGGTTCTGAGAAATTT+CGG	-	chr1.2:3139341-3139360	MS.gene27370:CDS	35.0%
!!	TTTTCGAAAGGTACAGCTAA+TGG	+	chr1.2:3139388-3139407	None:intergenic	35.0%
!!!	CCTTTAAGGTTCACATTTTC+AGG	+	chr1.2:3139019-3139038	None:intergenic	35.0%
	AAAAGGTCATACTGAGCAAG+AGG	-	chr1.2:3139252-3139271	MS.gene27370:CDS	40.0%
	AAAGGTCATACTGAGCAAGA+GGG	-	chr1.2:3139253-3139272	MS.gene27370:CDS	40.0%
	AACTTCGTTAACAGGCTTCT+TGG	+	chr1.2:3139518-3139537	None:intergenic	40.0%
	AGTGGAGTTGTGTGGAATAT+AGG	-	chr1.2:3139043-3139062	MS.gene27370:CDS	40.0%
	ATATTCCACACAACTCCACT+AGG	+	chr1.2:3139043-3139062	None:intergenic	40.0%
	CCAACCGTTTGTGAAGTAAA+CGG	+	chr1.2:3139089-3139108	None:intergenic	40.0%
	GAAGTCAACACTGCTTCTAA+TGG	-	chr1.2:3139310-3139329	MS.gene27370:CDS	40.0%
	GACACCGTTTACTTCACAAA+CGG	-	chr1.2:3139082-3139101	MS.gene27370:CDS	40.0%
	GCGAGAAAGCAATTGTTGAT+TGG	-	chr1.2:3139590-3139609	MS.gene27370:CDS	40.0%
	GTGGAGTTGTGTGGAATATA+GGG	-	chr1.2:3139044-3139063	MS.gene27370:CDS	40.0%
!	GAAAGCAATTGTTGATTGGG+TGG	-	chr1.2:3139594-3139613	MS.gene27370:CDS	40.0%
	CAACTCCACTAGGACCTTTA+AGG	+	chr1.2:3139033-3139052	None:intergenic	45.0%
	CCAGCAAAATCAGCTACTTC+TGG	-	chr1.2:3139568-3139587	MS.gene27370:CDS	45.0%
	CCGTTTACTTCACAAACGGT+TGG	-	chr1.2:3139086-3139105	MS.gene27370:CDS	45.0%
	GCTTGTAGCAAGGAAAACTC+AGG	-	chr1.2:3139616-3139635	MS.gene27370:CDS	45.0%
	GTCAACACTGCTTCTAATGG+CGG	-	chr1.2:3139313-3139332	MS.gene27370:CDS	45.0%
	GTGAACCTTAAAGGTCCTAG+TGG	-	chr1.2:3139025-3139044	MS.gene27370:CDS	45.0%
	TACTTCACAAACGGTTGGCA+TGG	-	chr1.2:3139091-3139110	MS.gene27370:CDS	45.0%
!	TCTAATGGCGGTGTTGAATG+CGG	-	chr1.2:3139325-3139344	MS.gene27370:CDS	45.0%
!!	AGGCTTCTTGGTCTTTTTGC+CGG	+	chr1.2:3139506-3139525	None:intergenic	45.0%
!!	CCAGAAGTAGCTGATTTTGC+TGG	+	chr1.2:3139571-3139590	None:intergenic	45.0%
!!	GGTCTTTTTGCCGGTTGTTT+GGG	+	chr1.2:3139497-3139516	None:intergenic	45.0%
!!	TGGTCTTTTTGCCGGTTGTT+TGG	+	chr1.2:3139498-3139517	None:intergenic	45.0%
!!	TTTTTGCCGGTTGTTTGGGA+GGG	+	chr1.2:3139493-3139512	None:intergenic	45.0%
	AAGGTCCTAGTGGAGTTGTG+TGG	-	chr1.2:3139035-3139054	MS.gene27370:CDS	50.0%
	AGAGCCTGCTTTCTCGCATA+AGG	+	chr1.2:3139212-3139231	None:intergenic	50.0%
	CCAGGCGTAACTTCGTTAAC+AGG	+	chr1.2:3139526-3139545	None:intergenic	50.0%
	CCTGTTAACGAAGTTACGCC+TGG	-	chr1.2:3139523-3139542	MS.gene27370:CDS	50.0%
	GAGACCTTATGCGAGAAAGC+AGG	-	chr1.2:3139205-3139224	MS.gene27370:CDS	50.0%
	GGCTGATGCAGTCACTATTG+CGG	-	chr1.2:3139465-3139484	MS.gene27370:CDS	50.0%
!	TGATTGGGTGGCTTGTAGCA+AGG	-	chr1.2:3139606-3139625	MS.gene27370:CDS	50.0%
!!	CTTTTTGCCGGTTGTTTGGG+AGG	+	chr1.2:3139494-3139513	None:intergenic	50.0%
	ATCAGCCATGACCTGCTCAG+GGG	+	chr1.2:3139452-3139471	None:intergenic	55.0%
	CATCAGCCATGACCTGCTCA+GGG	+	chr1.2:3139453-3139472	None:intergenic	55.0%
	TACTGAGCAAGAGGGCGACA+AGG	-	chr1.2:3139261-3139280	MS.gene27370:CDS	55.0%
	GCAAGAGGGCGACAAGGCAA+AGG	-	chr1.2:3139267-3139286	MS.gene27370:CDS	60.0%
	GCATCAGCCATGACCTGCTC+AGG	+	chr1.2:3139454-3139473	None:intergenic	60.0%
	GCGGTTCCCTCCCAAACAAC+CGG	-	chr1.2:3139484-3139503	MS.gene27370:CDS	60.0%
	GTCTTCCCCGCTTCTTAGGC+TGG	+	chr1.2:3139549-3139568	None:intergenic	60.0%
	TGAGTCTGCTTCCCCTGAGC+AGG	-	chr1.2:3139438-3139457	MS.gene27370:CDS	60.0%
	TGCTTCCCCTGAGCAGGTCA+TGG	-	chr1.2:3139444-3139463	MS.gene27370:CDS	60.0%
!!	GCTGGTCTTCCCCGCTTCTT+AGG	+	chr1.2:3139553-3139572	None:intergenic	60.0%
	CCTGGCCAGCCTAAGAAGCG+GGG	-	chr1.2:3139541-3139560	MS.gene27370:CDS	65.0%
	CGCCTGGCCAGCCTAAGAAG+CGG	-	chr1.2:3139539-3139558	MS.gene27370:CDS	65.0%
	GCCTGGCCAGCCTAAGAAGC+GGG	-	chr1.2:3139540-3139559	MS.gene27370:CDS	65.0%
!	CCCCGCTTCTTAGGCTGGCC+AGG	+	chr1.2:3139544-3139563	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	3138974	3139637	3138974	ID=MS.gene27370
chr1.2	mRNA	3138974	3139637	3138974	ID=MS.gene27370.t1;Parent=MS.gene27370
chr1.2	exon	3138974	3139637	3138974	ID=MS.gene27370.t1.exon1;Parent=MS.gene27370.t1
chr1.2	CDS	3138974	3139637	3138974	ID=cds.MS.gene27370.t1;Parent=MS.gene27370.t1

Gene Sequence

>MS.gene27370

GCACTTCCTAAAACATTTTCAGACAATTTAAAGAAGAAGTTGCCTGAAAATGTGAACCTTAAAGGTCCTAGTGGAGTTGTGTGGAATATAGGGCTGACAACTAAAGATGACACCGTTTACTTCACAAACGGTTGGCATGGATTTGTGAATGATCACTCTTTGAAAGAGAGTGATCTCCTTTTTTTCAAGTACAATGGCGAATCGTTGTTTGAAGTTTTAATATTTCATGGCGAGACCTTATGCGAGAAAGCAGGCTCTTACTTTGTTAGAAAATGTGGAAAAGGTCATACTGAGCAAGAGGGCGACAAGGCAAAGGTTGCAAAGAATTCTGTTGAAGAAGTCAACACTGCTTCTAATGGCGGTGTTGAATGCGGTTCTGAGAAATTTCGGAAGCTTGACACTATAAGAACACCATTAGCTGTACCTTTCGAAAATACCAATGAAAAGACTTCTAATGCTGGTGTTGAGTCTGCTTCCCCTGAGCAGGTCATGGCTGATGCAGTCACTATTGCGGTTCCCTCCCAAACAACCGGCAAAAAGACCAAGAAGCCTGTTAACGAAGTTACGCCTGGCCAGCCTAAGAAGCGGGGAAGACCAGCAAAATCAGCTACTTCTGGCGAGAAAGCAATTGTTGATTGGGTGGCTTGTAGCAAGGAAAACTCAG

Protein sequence

>MS.gene27370.t1

ALPKTFSDNLKKKLPENVNLKGPSGVVWNIGLTTKDDTVYFTNGWHGFVNDHSLKESDLLFFKYNGESLFEVLIFHGETLCEKAGSYFVRKCGKGHTEQEGDKAKVAKNSVEEVNTASNGGVECGSEKFRKLDTIRTPLAVPFENTNEKTSNAGVESASPEQVMADAVTIAVPSQTTGKKTKKPVNEVTPGQPKKRGRPAKSATSGEKAIVDWVACSKENS