AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24342


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24342.t1 MTR_1g021440 65.179 224 69 3 1 221 37 254 1.64e-96 283
MS.gene24342.t1 MTR_1g021320 60.674 267 58 4 1 221 197 462 2.41e-95 296
MS.gene24342.t1 MTR_1g021320 77.419 124 28 0 1 124 40 163 6.14e-62 207
MS.gene24342.t1 MTR_1g021440 65.179 224 69 3 1 221 37 254 6.25e-95 283
MS.gene24342.t1 MTR_1g021440 67.647 204 58 2 1 201 37 235 5.54e-94 276
MS.gene24342.t1 MTR_1g021440 67.647 204 58 2 1 201 37 235 1.71e-92 276
MS.gene24342.t1 MTR_1g021350 62.115 227 58 6 1 221 36 240 8.78e-86 258
MS.gene24342.t1 MTR_1g021330 62.162 222 55 4 1 221 41 234 4.34e-84 254
MS.gene24342.t1 MTR_1g021435 60.889 225 45 5 1 221 37 222 3.57e-79 242
MS.gene24342.t1 MTR_1g021400 58.108 222 55 3 1 221 45 229 1.70e-73 227
MS.gene24342.t1 MTR_1g021380 56.696 224 50 5 1 221 36 215 5.35e-73 225
MS.gene24342.t1 MTR_1g021410 57.143 224 55 5 1 221 37 222 3.38e-68 213
MS.gene24342.t1 MTR_1g021500 52.423 227 76 6 1 214 41 248 1.16e-62 201
MS.gene24342.t1 MTR_1g021500 52.423 227 76 6 1 214 41 248 2.57e-62 202
MS.gene24342.t1 MTR_1g021360 74.400 125 32 0 1 125 40 164 7.03e-61 197
MS.gene24342.t1 MTR_1g021360 54.412 204 41 4 1 203 197 349 8.60e-61 197
MS.gene24342.t1 MTR_1g021290 69.421 121 37 0 1 121 170 290 7.64e-53 173
MS.gene24342.t1 MTR_1g021290 74.257 101 26 0 1 101 42 142 3.01e-49 164
MS.gene24342.t1 MTR_1g021270 38.372 86 52 1 2 87 31 115 1.51e-14 72.4
MS.gene24342.t1 MTR_3g098790 37.500 80 50 0 1 80 28 107 2.27e-12 65.5
MS.gene24342.t1 MTR_7g050710 37.838 74 46 0 2 75 29 102 2.29e-12 65.5
MS.gene24342.t1 MTR_7g050640 37.333 75 47 0 1 75 28 102 3.38e-12 61.6
MS.gene24342.t1 MTR_7g050580 37.975 79 49 0 2 80 29 107 4.21e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24342.t1 AT4G33280 56.250 96 41 1 1 95 38 133 3.66e-31 115
MS.gene24342.t1 AT4G33280 56.250 96 41 1 1 95 38 133 9.00e-31 115
MS.gene24342.t1 AT4G33280 56.250 96 41 1 1 95 38 133 1.94e-30 116
MS.gene24342.t1 AT4G34400 39.759 83 50 0 1 83 30 112 1.66e-17 80.5
MS.gene24342.t1 AT4G34400 39.759 83 50 0 1 83 30 112 1.66e-17 80.5
MS.gene24342.t1 AT3G18990 35.714 98 54 1 2 90 22 119 1.74e-13 68.9
MS.gene24342.t1 AT3G18990 35.714 98 54 1 2 90 22 119 1.85e-13 68.9
MS.gene24342.t1 AT3G06220 33.784 74 49 0 2 75 25 98 4.78e-13 65.5
MS.gene24342.t1 AT5G66980 41.772 79 42 1 1 75 24 102 5.36e-12 64.3
MS.gene24342.t1 AT5G60130 38.636 88 49 2 1 83 28 115 7.30e-12 63.2
MS.gene24342.t1 AT3G06160 36.000 75 48 0 1 75 39 113 7.82e-12 63.9
MS.gene24342.t1 AT3G06160 36.000 75 48 0 1 75 24 98 9.09e-12 63.5
MS.gene24342.t1 AT3G06160 36.000 75 48 0 1 75 24 98 9.09e-12 63.5
MS.gene24342.t1 AT3G06160 36.000 75 48 0 1 75 24 98 9.09e-12 63.5
MS.gene24342.t1 AT3G06160 36.000 75 48 0 1 75 83 157 1.00e-11 63.9
MS.gene24342.t1 AT5G60130 38.636 88 49 2 1 83 2 89 1.22e-11 63.2
MS.gene24342.t1 AT5G60130 38.636 88 49 2 1 83 25 112 1.60e-11 63.2
MS.gene24342.t1 AT5G60130 38.636 88 49 2 1 83 28 115 1.64e-11 63.2
MS.gene24342.t1 AT5G60130 38.636 88 49 2 1 83 28 115 1.76e-11 62.8

Find 46 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTCCACTAGGACCTTTA+AGG 0.183323 1.1:+2896090 None:intergenic
CCTGAAAATGTGAACCTTAA+AGG 0.211774 1.1:-2896104 MS.gene24342:CDS
GCTGGTCTTCCCCGCTTCTT+AGG 0.270434 1.1:+2895570 None:intergenic
AGTGGAGTTGTGTGGAATAT+AGG 0.300231 1.1:-2896077 MS.gene24342:CDS
AACTTCGTTAACAGGCTTCT+TGG 0.341598 1.1:+2895605 None:intergenic
CCAGGCGTAACTTCGTTAAC+AGG 0.352395 1.1:+2895597 None:intergenic
GTCTTCCCCGCTTCTTAGGC+TGG 0.353235 1.1:+2895574 None:intergenic
AATGCGGTTCTGAGAAATTT+CGG 0.363117 1.1:-2895779 MS.gene24342:CDS
CCCCGCTTCTTAGGCTGGCC+AGG 0.381562 1.1:+2895579 None:intergenic
CCAGCAAAATCAGCTACTTC+TGG 0.385623 1.1:-2895552 MS.gene24342:CDS
GTGGAGTTGTGTGGAATATA+GGG 0.421556 1.1:-2896076 MS.gene24342:CDS
TTTGTTAGAAAATGTGGAAA+AGG 0.421612 1.1:-2895885 MS.gene24342:CDS
GCATCAGCCATGACCTGCTC+AGG 0.424246 1.1:+2895669 None:intergenic
TGAGTCTGCTTCCCCTGAGC+AGG 0.449413 1.1:-2895682 MS.gene24342:CDS
GCGAGAAAGCAATTGTTGAT+TGG 0.450466 1.1:-2895530 MS.gene24342:CDS
TGCTTCCCCTGAGCAGGTCA+TGG 0.458901 1.1:-2895676 MS.gene24342:CDS
GAAGTCAACACTGCTTCTAA+TGG 0.464985 1.1:-2895810 MS.gene24342:CDS
GCCTGGCCAGCCTAAGAAGC+GGG 0.466869 1.1:-2895580 MS.gene24342:CDS
AGAGCCTGCTTTCTCGCATA+AGG 0.473371 1.1:+2895911 None:intergenic
CCAACCGTTTGTGAAGTAAA+CGG 0.478109 1.1:+2896034 None:intergenic
CGAGAAAGCAATTGTTGATT+GGG 0.485091 1.1:-2895529 MS.gene24342:CDS
GCGGTTCCCTCCCAAACAAC+CGG 0.485847 1.1:-2895636 MS.gene24342:CDS
TCTTACTTTGTTAGAAAATG+TGG 0.486876 1.1:-2895891 MS.gene24342:CDS
GACACCGTTTACTTCACAAA+CGG 0.514012 1.1:-2896038 MS.gene24342:CDS
TACTTCACAAACGGTTGGCA+AGG 0.517034 1.1:-2896029 MS.gene24342:CDS
CCTGTTAACGAAGTTACGCC+TGG 0.535294 1.1:-2895597 MS.gene24342:CDS
AATGAAAAGACTTCTAATGC+TGG 0.546042 1.1:-2895708 MS.gene24342:CDS
CCGTTTACTTCACAAACGGT+TGG 0.558722 1.1:-2896034 MS.gene24342:CDS
CATCAGCCATGACCTGCTCA+GGG 0.563227 1.1:+2895670 None:intergenic
CGCCTGGCCAGCCTAAGAAG+CGG 0.563693 1.1:-2895581 MS.gene24342:CDS
GAAAGCAATTGTTGATTGGG+TGG 0.568458 1.1:-2895526 MS.gene24342:CDS
ATATTCCACACAACTCCACT+AGG 0.578077 1.1:+2896080 None:intergenic
GAGACCTTATGCGAGAAAGC+AGG 0.587625 1.1:-2895915 MS.gene24342:CDS
GCAAGAGGGCGACAAGGCAA+AGG 0.587651 1.1:-2895853 MS.gene24342:CDS
GGCTGATGCAGTCACTATTG+CGG 0.596142 1.1:-2895655 MS.gene24342:CDS
TACTGAGCAAGAGGGCGACA+AGG 0.599125 1.1:-2895859 MS.gene24342:CDS
GTGAACCTTAAAGGTCCTAG+TGG 0.609317 1.1:-2896095 MS.gene24342:CDS
AAAAGGTCATACTGAGCAAG+AGG 0.615156 1.1:-2895868 MS.gene24342:CDS
AAGGTCCTAGTGGAGTTGTG+TGG 0.624686 1.1:-2896085 MS.gene24342:CDS
GCTTGTAGCAAGGAAAACTC+AGG 0.631925 1.1:-2895504 None:intergenic
CCTGGCCAGCCTAAGAAGCG+GGG 0.640983 1.1:-2895579 MS.gene24342:CDS
GTCAACACTGCTTCTAATGG+CGG 0.645750 1.1:-2895807 MS.gene24342:CDS
TGATTGGGTGGCTTGTAGCA+AGG 0.654638 1.1:-2895514 MS.gene24342:CDS
TCTAATGGCGGTGTTGAATG+CGG 0.661026 1.1:-2895795 MS.gene24342:CDS
AAAGGTCATACTGAGCAAGA+GGG 0.680883 1.1:-2895867 MS.gene24342:CDS
ATCAGCCATGACCTGCTCAG+GGG 0.726483 1.1:+2895671 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGAAGTTTTAATATTTCA+TGG - chr1.1:2895712-2895731 MS.gene24342:CDS 15.0%
!!! AAATTGTCTGAAAATGTTTT+AGG + chr1.1:2895514-2895533 None:intergenic 20.0%
! TCTTACTTTGTTAGAAAATG+TGG - chr1.1:2895760-2895779 MS.gene24342:CDS 25.0%
! TTTGTTAGAAAATGTGGAAA+AGG - chr1.1:2895766-2895785 MS.gene24342:CDS 25.0%
!! TTTTCATTGGTATTTTCGAA+AGG + chr1.1:2895931-2895950 None:intergenic 25.0%
AATGAAAAGACTTCTAATGC+TGG - chr1.1:2895943-2895962 MS.gene24342:CDS 30.0%
! AGCATTAGAAGTCTTTTCAT+TGG + chr1.1:2895944-2895963 None:intergenic 30.0%
!!! CTCCTTTTTTTCAAGTACAA+TGG - chr1.1:2895679-2895698 MS.gene24342:CDS 30.0%
CCTGAAAATGTGAACCTTAA+AGG - chr1.1:2895547-2895566 MS.gene24342:CDS 35.0%
CGCCATTGTACTTGAAAAAA+AGG + chr1.1:2895684-2895703 None:intergenic 35.0%
! CGAGAAAGCAATTGTTGATT+GGG - chr1.1:2896122-2896141 MS.gene24342:CDS 35.0%
!! AATGCGGTTCTGAGAAATTT+CGG - chr1.1:2895872-2895891 MS.gene24342:CDS 35.0%
!! TTTTCGAAAGGTACAGCTAA+TGG + chr1.1:2895919-2895938 None:intergenic 35.0%
!!! CCTTTAAGGTTCACATTTTC+AGG + chr1.1:2895550-2895569 None:intergenic 35.0%
AAAAGGTCATACTGAGCAAG+AGG - chr1.1:2895783-2895802 MS.gene24342:CDS 40.0%
AAAGGTCATACTGAGCAAGA+GGG - chr1.1:2895784-2895803 MS.gene24342:CDS 40.0%
AACTTCGTTAACAGGCTTCT+TGG + chr1.1:2896049-2896068 None:intergenic 40.0%
AGTGGAGTTGTGTGGAATAT+AGG - chr1.1:2895574-2895593 MS.gene24342:CDS 40.0%
ATATTCCACACAACTCCACT+AGG + chr1.1:2895574-2895593 None:intergenic 40.0%
CCAACCGTTTGTGAAGTAAA+CGG + chr1.1:2895620-2895639 None:intergenic 40.0%
GAAGTCAACACTGCTTCTAA+TGG - chr1.1:2895841-2895860 MS.gene24342:CDS 40.0%
GACACCGTTTACTTCACAAA+CGG - chr1.1:2895613-2895632 MS.gene24342:CDS 40.0%
GCGAGAAAGCAATTGTTGAT+TGG - chr1.1:2896121-2896140 MS.gene24342:CDS 40.0%
GTGGAGTTGTGTGGAATATA+GGG - chr1.1:2895575-2895594 MS.gene24342:CDS 40.0%
! GAAAGCAATTGTTGATTGGG+TGG - chr1.1:2896125-2896144 MS.gene24342:CDS 40.0%
CAACTCCACTAGGACCTTTA+AGG + chr1.1:2895564-2895583 None:intergenic 45.0%
CCAGCAAAATCAGCTACTTC+TGG - chr1.1:2896099-2896118 MS.gene24342:CDS 45.0%
CCGTTTACTTCACAAACGGT+TGG - chr1.1:2895617-2895636 MS.gene24342:CDS 45.0%
GCTTGTAGCAAGGAAAACTC+AGG - chr1.1:2896147-2896166 MS.gene24342:CDS 45.0%
GTCAACACTGCTTCTAATGG+CGG - chr1.1:2895844-2895863 MS.gene24342:CDS 45.0%
GTGAACCTTAAAGGTCCTAG+TGG - chr1.1:2895556-2895575 MS.gene24342:CDS 45.0%
TACTTCACAAACGGTTGGCA+AGG - chr1.1:2895622-2895641 MS.gene24342:CDS 45.0%
! TCTAATGGCGGTGTTGAATG+CGG - chr1.1:2895856-2895875 MS.gene24342:CDS 45.0%
!! AGGCTTCTTGGTCTTTTTGC+CGG + chr1.1:2896037-2896056 None:intergenic 45.0%
!! CCAGAAGTAGCTGATTTTGC+TGG + chr1.1:2896102-2896121 None:intergenic 45.0%
!! GGTCTTTTTGCCGGTTGTTT+GGG + chr1.1:2896028-2896047 None:intergenic 45.0%
!! TGGTCTTTTTGCCGGTTGTT+TGG + chr1.1:2896029-2896048 None:intergenic 45.0%
!! TTTTTGCCGGTTGTTTGGGA+GGG + chr1.1:2896024-2896043 None:intergenic 45.0%
AAGGTCCTAGTGGAGTTGTG+TGG - chr1.1:2895566-2895585 MS.gene24342:CDS 50.0%
AGAGCCTGCTTTCTCGCATA+AGG + chr1.1:2895743-2895762 None:intergenic 50.0%
CCAGGCGTAACTTCGTTAAC+AGG + chr1.1:2896057-2896076 None:intergenic 50.0%
CCTGTTAACGAAGTTACGCC+TGG - chr1.1:2896054-2896073 MS.gene24342:CDS 50.0%
GAGACCTTATGCGAGAAAGC+AGG - chr1.1:2895736-2895755 MS.gene24342:CDS 50.0%
GGCTGATGCAGTCACTATTG+CGG - chr1.1:2895996-2896015 MS.gene24342:CDS 50.0%
! TGATTGGGTGGCTTGTAGCA+AGG - chr1.1:2896137-2896156 MS.gene24342:CDS 50.0%
!! CTTTTTGCCGGTTGTTTGGG+AGG + chr1.1:2896025-2896044 None:intergenic 50.0%
ATCAGCCATGACCTGCTCAG+GGG + chr1.1:2895983-2896002 None:intergenic 55.0%
CATCAGCCATGACCTGCTCA+GGG + chr1.1:2895984-2896003 None:intergenic 55.0%
TACTGAGCAAGAGGGCGACA+AGG - chr1.1:2895792-2895811 MS.gene24342:CDS 55.0%
GCAAGAGGGCGACAAGGCAA+AGG - chr1.1:2895798-2895817 MS.gene24342:CDS 60.0%
GCATCAGCCATGACCTGCTC+AGG + chr1.1:2895985-2896004 None:intergenic 60.0%
GCGGTTCCCTCCCAAACAAC+CGG - chr1.1:2896015-2896034 MS.gene24342:CDS 60.0%
GTCTTCCCCGCTTCTTAGGC+TGG + chr1.1:2896080-2896099 None:intergenic 60.0%
TGAGTCTGCTTCCCCTGAGC+AGG - chr1.1:2895969-2895988 MS.gene24342:CDS 60.0%
TGCTTCCCCTGAGCAGGTCA+TGG - chr1.1:2895975-2895994 MS.gene24342:CDS 60.0%
!! GCTGGTCTTCCCCGCTTCTT+AGG + chr1.1:2896084-2896103 None:intergenic 60.0%
CCTGGCCAGCCTAAGAAGCG+GGG - chr1.1:2896072-2896091 MS.gene24342:CDS 65.0%
CGCCTGGCCAGCCTAAGAAG+CGG - chr1.1:2896070-2896089 MS.gene24342:CDS 65.0%
GCCTGGCCAGCCTAAGAAGC+GGG - chr1.1:2896071-2896090 MS.gene24342:CDS 65.0%
! CCCCGCTTCTTAGGCTGGCC+AGG + chr1.1:2896075-2896094 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 2895505 2896168 2895505 ID=MS.gene24342
chr1.1 mRNA 2895505 2896168 2895505 ID=MS.gene24342.t1;Parent=MS.gene24342
chr1.1 exon 2895505 2896168 2895505 ID=MS.gene24342.t1.exon1;Parent=MS.gene24342.t1
chr1.1 CDS 2895505 2896168 2895505 ID=cds.MS.gene24342.t1;Parent=MS.gene24342.t1
Gene Sequence

>MS.gene24342

GCACTTCCTAAAACATTTTCAGACAATTTAAAGAAGAAGTTGCCTGAAAATGTGAACCTTAAAGGTCCTAGTGGAGTTGTGTGGAATATAGGGCTGACAACTAAAGATGACACCGTTTACTTCACAAACGGTTGGCAAGGATTTGTGAATGATCACTCTTTGAAAGAGAGTGATCTCCTTTTTTTCAAGTACAATGGCGAATCGTTGTTTGAAGTTTTAATATTTCATGGCGAGACCTTATGCGAGAAAGCAGGCTCTTACTTTGTTAGAAAATGTGGAAAAGGTCATACTGAGCAAGAGGGCGACAAGGCAAAGGTTGCAAAGAATTCTGTTGAAGAAGTCAACACTGCTTCTAATGGCGGTGTTGAATGCGGTTCTGAGAAATTTCGGAAGCTTGACACTATAAGAACACCATTAGCTGTACCTTTCGAAAATACCAATGAAAAGACTTCTAATGCTGGTGTTGAGTCTGCTTCCCCTGAGCAGGTCATGGCTGATGCAGTCACTATTGCGGTTCCCTCCCAAACAACCGGCAAAAAGACCAAGAAGCCTGTTAACGAAGTTACGCCTGGCCAGCCTAAGAAGCGGGGAAGACCAGCAAAATCAGCTACTTCTGGCGAGAAAGCAATTGTTGATTGGGTGGCTTGTAGCAAGGAAAACTCAG

Protein sequence

>MS.gene24342.t1

ALPKTFSDNLKKKLPENVNLKGPSGVVWNIGLTTKDDTVYFTNGWQGFVNDHSLKESDLLFFKYNGESLFEVLIFHGETLCEKAGSYFVRKCGKGHTEQEGDKAKVAKNSVEEVNTASNGGVECGSEKFRKLDTIRTPLAVPFENTNEKTSNAGVESASPEQVMADAVTIAVPSQTTGKKTKKPVNEVTPGQPKKRGRPAKSATSGEKAIVDWVACSKENS