AlfalfaGEDB | MS.gene67076

M. sativa cultivar XinJiangDaYe / MS.gene67076

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67076.t1	XP_003589268.3	93.7	237	13	2	1	236	170	405	3.10E-121	444.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67076.t1	Q8RYD1	46.7	120	61	3	2	119	39	157	6.2e-22	105.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67076.t1	A0A396JGJ9	93.7	237	13	2	1	236	170	405	2.2e-121	444.5

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene67076.t1	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene24348	MS.gene67076	PPI
MS.gene67073	MS.gene67076	PPI
MS.gene67076	MS.gene24348	PPI
MS.gene67076	MS.gene043976	PPI
MS.gene67076	MS.gene27370	PPI
MS.gene67076	MS.gene043977	PPI
MS.gene67076	MS.gene24349	PPI
MS.gene67076	MS.gene67073	PPI
MS.gene67076	MS.gene24342	PPI
MS.gene67076	MS.gene67075	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67076.t1	MTR_1g021290	93.258	178	10	2	1	177	170	346	7.47e-112	325
MS.gene67076.t1	MTR_1g021290	72.549	102	27	1	1	102	42	142	1.05e-45	155
MS.gene67076.t1	MTR_1g021320	50.307	326	71	4	1	236	197	521	2.44e-89	281
MS.gene67076.t1	MTR_1g021320	70.248	121	35	1	1	121	40	159	1.44e-49	173
MS.gene67076.t1	MTR_1g021435	59.350	246	72	4	1	236	37	264	2.62e-86	261
MS.gene67076.t1	MTR_1g021440	51.079	278	76	5	1	236	37	296	2.40e-80	243
MS.gene67076.t1	MTR_1g021400	56.972	251	69	6	1	236	45	271	3.23e-79	242
MS.gene67076.t1	MTR_1g021440	51.079	278	76	5	1	236	37	296	1.05e-78	242
MS.gene67076.t1	MTR_1g021410	55.020	249	78	5	1	236	37	264	1.84e-76	235
MS.gene67076.t1	MTR_1g021440	51.128	266	76	5	1	236	37	278	5.68e-76	231

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67076.t1	AT4G33280	50.980	102	48	2	1	101	38	138	1.33e-28	109
MS.gene67076.t1	AT4G33280	44.628	121	64	3	1	119	38	157	3.57e-28	109
MS.gene67076.t1	AT4G33280	50.980	102	48	2	1	101	38	138	5.64e-28	110
MS.gene67076.t1	AT4G34400	35.714	84	53	1	1	84	30	112	8.46e-13	67.4
MS.gene67076.t1	AT4G34400	35.714	84	53	1	1	84	30	112	8.46e-13	67.4

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Find 51 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTCAACAGCACTTGTTG+AGG	0.643971	1.2:-3459557	MS.gene67076:CDS
AAATGAGGAGGAAAATGATT+GGG	0.529211	1.2:+3459525	None:intergenic
AACAAAATATGGAAATGAGG+AGG	0.576763	1.2:+3459513	None:intergenic
AACTCCACTAGGACCTTTAA+GGG	0.495173	1.2:+3461029	None:intergenic
AAGCTCCGCTGAAAATACAA+TGG	0.674487	1.2:+3459587	None:intergenic
AAGGTCCTAGTGGAGTTACA+TGG	0.559188	1.2:-3461023	MS.gene67076:CDS
ACGAGAACCACTGACATTGA+AGG	0.493660	1.2:+3459477	None:intergenic
ACTGAATAGCTGCGGTCTTA+GGG	0.455599	1.2:+3460686	None:intergenic
ACTTGTAACAAGCAACACCC+AGG	0.683080	1.2:-3460574	MS.gene67076:intron
AGATGGCACTGTTTACTTCG+TGG	0.564148	1.2:-3460977	MS.gene67076:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATGAGGAGGAAAATGATT+GGG	+	chr1.2:3461033-3461052	None:intergenic	30.0%
AACAAAATATGGAAATGAGG+AGG	+	chr1.2:3461045-3461064	None:intergenic	30.0%
AAGAGAAGTAGTATTATCGA+TGG	+	chr1.2:3460573-3460592	None:intergenic	30.0%
AGACAGTTTAATAGCCATTA+AGG	+	chr1.2:3460610-3460629	None:intergenic	30.0%
GACAGTTTAATAGCCATTAA+GGG	+	chr1.2:3460609-3460628	None:intergenic	30.0%
GAGTTACATGGAAGAATATA+AGG	-	chr1.2:3459544-3459563	MS.gene67076:CDS	30.0%
GATGTTCATACATGATGTAT+CGG	-	chr1.2:3460231-3460250	MS.gene67076:intron	30.0%
TAAGCTCTGTTATGAATATC+TGG	+	chr1.2:3460868-3460887	None:intergenic	30.0%
TTATGTCATCCTTCACAAAT+AGG	+	chr1.2:3460479-3460498	None:intergenic	30.0%
TTTAATAGCCATTAAGGGAA+AGG	+	chr1.2:3460604-3460623	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	3459471	3461106	3459471	ID=MS.gene67076
chr1.2	mRNA	3459471	3461106	3459471	ID=MS.gene67076.t1;Parent=MS.gene67076
chr1.2	exon	3460575	3461106	3460575	ID=MS.gene67076.t1.exon1;Parent=MS.gene67076.t1
chr1.2	CDS	3460575	3461106	3460575	ID=cds.MS.gene67076.t1;Parent=MS.gene67076.t1
chr1.2	exon	3459471	3459646	3459471	ID=MS.gene67076.t1.exon2;Parent=MS.gene67076.t1
chr1.2	CDS	3459471	3459646	3459471	ID=cds.MS.gene67076.t1;Parent=MS.gene67076.t1

Gene Sequence

>MS.gene67076

GCACTTCCTAAAACATTTTCAGACAATTTAAATAAGAAGTTGCCAGAAAATTTGACCCTTAAAGGTCCTAGTGGAGTTACATGGAAGAATATAAGGCTGACAACTAGAGATGGCACTGTTTACTTCGTGGACGGTTGGCAACAATTCATGAACGATCACTCGTTGAAAGCTAATGATTTCCTTGTCTGTAAGTACAATGGTGAATCACACTTTGAGGTTTTAATCTTTGATGGAGAAAGCTTCTGCGAGAAAGAAGCTTCTTATTTTGTCGAAAAATGCGGCCATGCTCAAACAGCACAAGGGGGTAGCAATGCATCGCAAACAAACAATTCTGTTGAAGAAGTCAATACTGCTTCTAATGCTGGTGATTCCCCTGAGCAGTTCACGGATGATGCAGTCCCTAAGACCGCAGCTATTCAGTGTCCCTTCCTACCAACCGGCAAAAGAACCAAGAGGAGAAGAAGATCACCAAAAGCTGCTAATTGGGGTGCGAGAGCACATGCTTGGGTGACTTGTAACAAGCAACACCCAGGTTAAAATATTTCTTTCTTTTCCATTAACTTTTACGATATTAAATGGAACCCAGTCAATACGAATATCTAGTGTGTATCTATGTTGACTATGATAAATTCTGATTTATATTCATATTGAATCACTAGCTAAACTTAAAGCAGCTAACCATTTTTTTTTTGGTTAACTTTGTATAACCAACCACTTTTCTTTTTTCATTTGATTTATGACTTGAATCATGTATTATTTTGATGTTCATACATGATGTATCGGCTTATAGTTTTGTTGTTATGTCACATGCTTTTACTTAAAAATGACATATCGGCCTAGTTCAAATAAATCAATTTTTCACTACCTCTATATATATTCCTCTCTCCCATGTGTGTTTCTCTCCCTATCTCTAGCTAACTTGCCAACTAAGCACTTTTATCTCGAGTGTTGTCAAATATCGGCCATGGCGGATTGTGGTGGTGGATTTTTTAACAACCGGCATGACCTATTTGTGAAGGATGACATAACCATGGTGTTTTGTGGTGGATATGGCGGCGCCTATGGGGATTTTTGGCTTTCCGTCACGGTCTGCCATCCGCCATCGATAATACTACTTCTCTTCATTTTGGCCTTTCCCTTAATGGCTATTAAACTGTCTCTCAGTCAAGTAATCTCTGGTAACCTTAATATCATCAACATAAACAAGAACTACAATTAGTTTTCCTCCTTGTGAATGTTTGAACTAAAGATTTTGATTTCCTTGACTTTTCTTATAGCCCAAGCACACCACTGCATTTGATTTCATTCTGGTTCTCAAAAATTTGTATTATAACTCATCTGATCTTCCCTAGCAAACTGATACTTGTCTAGGAAAAAGTATTTTGCATTCAAATCCAGATATTCATAACAGAGCTTATCCTTTTATTTGTAACTCATGATTGAACTGTTCAATTCCTCAGAGGCAGCCAGCCCGAATAGAAGTGGGAAGGTAGACGACCATTGTATTTTCAGCGGAGCTTCACTGTCAAAGTCAACAGCACTTGTTGAGGAAAAGATAGCCCAATCATTTTCCTCCTCATTTCCATATTTTGTTAAAATAATTAAAGCCTTCAATGTCAGTGGTTCTCGTACTATT

Protein sequence

>MS.gene67076.t1

ALPKTFSDNLNKKLPENLTLKGPSGVTWKNIRLTTRDGTVYFVDGWQQFMNDHSLKANDFLVCKYNGESHFEVLIFDGESFCEKEASYFVEKCGHAQTAQGGSNASQTNNSVEEVNTASNAGDSPEQFTDDAVPKTAAIQCPFLPTGKRTKRRRRSPKAANWGARAHAWVTCNKQHPEAASPNRSGKVDDHCIFSGASLSKSTALVEEKIAQSFSSSFPYFVKIIKAFNVSGSRTI